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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Nitric Oxide Synthase-Mediated Phytoalexin Accumulation in Soybean Cotyledons in Response to the <italic>Diaporthe phaseolorum</italic> f. sp. <italic>meridionalis</italic> Elicitor<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Modolo, Luzia Valentina" sort="Modolo, Luzia Valentina" uniqKey="Modolo L" first="Luzia Valentina" last="Modolo">Luzia Valentina Modolo</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author><author><name sortKey="Cunha, Fernando Queiroz" sort="Cunha, Fernando Queiroz" uniqKey="Cunha F" first="Fernando Queiroz" last="Cunha">Fernando Queiroz Cunha</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author><author><name sortKey="Braga, Marcia Regina" sort="Braga, Marcia Regina" uniqKey="Braga M" first="Márcia Regina" last="Braga">Márcia Regina Braga</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author><author><name sortKey="Salgado, Ione" sort="Salgado, Ione" uniqKey="Salgado I" first="Ione" last="Salgado">Ione Salgado</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12427995</idno><idno type="pmc">166649</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC166649</idno><idno type="RBID">PMC:166649</idno><idno type="doi">10.1104/pp.005850</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000532</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Nitric Oxide Synthase-Mediated Phytoalexin Accumulation in Soybean Cotyledons in Response to the <italic>Diaporthe phaseolorum</italic> f. sp. <italic>meridionalis</italic> Elicitor<xref ref-type="fn" rid="FN1">1</xref></title><author><name sortKey="Modolo, Luzia Valentina" sort="Modolo, Luzia Valentina" uniqKey="Modolo L" first="Luzia Valentina" last="Modolo">Luzia Valentina Modolo</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author><author><name sortKey="Cunha, Fernando Queiroz" sort="Cunha, Fernando Queiroz" uniqKey="Cunha F" first="Fernando Queiroz" last="Cunha">Fernando Queiroz Cunha</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author><author><name sortKey="Braga, Marcia Regina" sort="Braga, Marcia Regina" uniqKey="Braga M" first="Márcia Regina" last="Braga">Márcia Regina Braga</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author><author><name sortKey="Salgado, Ione" sort="Salgado, Ione" uniqKey="Salgado I" first="Ione" last="Salgado">Ione Salgado</name><affiliation><nlm:aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</nlm:aff></affiliation></author></analytic><series><title level="j">Plant Physiology</title><idno type="ISSN">0032-0889</idno><idno type="eISSN">1532-2548</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Phytoalexin biosynthesis is part of the defense mechanism of soybean (<italic>Glycine max</italic>) plants against attack by the fungus <italic>Diaporthe phaseolorum</italic> f. sp. <italic>meridionalis</italic> (Dpm), the causal agent of stem canker disease. The treatment of soybean cotyledons with Dpm elicitor or with sodium nitroprusside (SNP), a nitric oxide (NO) donor, resulted in a high accumulation of phytoalexins. This response did not occur when SNP was replaced by ferricyanide, a structural analog of SNP devoid of the NO moiety. Phytoalexin accumulation induced by the fungal elicitor, but not by SNP, was prevented when cotyledons were pretreated with NO synthase (NOS) inhibitors. The Dpm elicitor also induced NOS activity in soybean tissues proximal to the site of inoculation. The induced NOS activity was Ca<sup>2+</sup>- and NADPH-dependent and was sensitive to the NOS inhibitors N<sup><italic>G</italic></sup>-nitro-<sc>l</sc>-arginine methyl ester, aminoguanidine, and <sc>l</sc>-N<sup>6</sup>-(iminoethyl) lysine. NOS activity was not observed in SNP-elicited tissues. An antibody to brain NOS labeled a 166-kD protein in elicited and nonelicited cotyledons. Isoflavones (daidzein and genistein), pterocarpans (glyceollins), and flavones (apigenin and luteolin) were identified after exposure to the elicitor or SNP, although the accumulation of glyceollins and apigenin was limited in SNP-elicited compared with fungal-elicited cotyledons. NOS activity preceded the accumulation of these flavonoids in tissues treated with the Dpm elicitor. The accumulation of these metabolites was faster in SNP-elicited than in fungal-elicited cotyledons. We conclude that the response of soybean cotyledons to Dpm elicitor involves NO formation via a constitutive NOS-like enzyme that triggers the biosynthesis of antimicrobial flavonoids.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id><journal-id journal-id-type="publisher-id">PLANT PHYSIOL</journal-id><journal-title>Plant Physiology</journal-title><issn pub-type="ppub">0032-0889</issn><issn pub-type="epub">1532-2548</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12427995</article-id><article-id pub-id-type="pmc">166649</article-id><article-id pub-id-type="publisher-id">7526</article-id><article-id pub-id-type="doi">10.1104/pp.005850</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject><subj-group><subject>Plants Interacting with Other Organisms</subject></subj-group></subj-group></article-categories><title-group><article-title>Nitric Oxide Synthase-Mediated Phytoalexin Accumulation in Soybean Cotyledons in Response to the <italic>Diaporthe phaseolorum</italic> f. sp. <italic>meridionalis</italic> Elicitor<xref ref-type="fn" rid="FN1">1</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Modolo</surname><given-names>Luzia Valentina</given-names></name><xref ref-type="aff" rid="N0x97e6820N0x8c909d8"></xref></contrib><contrib contrib-type="author"><name><surname>Cunha</surname><given-names>Fernando Queiroz</given-names></name><xref ref-type="aff" rid="N0x97e6820N0x8c909d8"></xref></contrib><contrib contrib-type="author"><name><surname>Braga</surname><given-names>Márcia Regina</given-names></name><xref ref-type="aff" rid="N0x97e6820N0x8c909d8"></xref></contrib><contrib contrib-type="author"><name><surname>Salgado</surname><given-names>Ione</given-names></name><xref ref-type="author-notes" rid="FN150">*</xref><xref ref-type="aff" rid="N0x97e6820N0x8c909d8"></xref></contrib></contrib-group><aff id="N0x97e6820N0x8c909d8">Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, 13083–970, Brazil (L.V.M., I.S.); Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14049–900, Brazil (F.Q.C.); and Seção de Fisiologia e Bioquímica de Plantas, Instituto de Botânica, São Paulo, 01061–970, SP, Brazil (M.R.B.)</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author; e-mail <email>ionesm@unicamp.br</email>; fax 55–19–3788–6129.</p></fn></author-notes><pub-date pub-type="ppub"><month>11</month><year>2002</year></pub-date><volume>130</volume><issue>3</issue><fpage>1288</fpage><lpage>1297</lpage><history><date date-type="received"><day>20</day><month>3</month><year>2002</year></date><date date-type="rev-recd"><day>27</day><month>5</month><year>2002</year></date><date date-type="accepted"><day>21</day><month>8</month><year>2002</year></date></history><copyright-statement>Copyright © 2002, American Society of Plant Biologists</copyright-statement><copyright-year>2002</copyright-year><abstract><p>Phytoalexin biosynthesis is part of the defense mechanism of soybean (<italic>Glycine max</italic>) plants against attack by the fungus <italic>Diaporthe phaseolorum</italic> f. sp. <italic>meridionalis</italic> (Dpm), the causal agent of stem canker disease. The treatment of soybean cotyledons with Dpm elicitor or with sodium nitroprusside (SNP), a nitric oxide (NO) donor, resulted in a high accumulation of phytoalexins. This response did not occur when SNP was replaced by ferricyanide, a structural analog of SNP devoid of the NO moiety. Phytoalexin accumulation induced by the fungal elicitor, but not by SNP, was prevented when cotyledons were pretreated with NO synthase (NOS) inhibitors. The Dpm elicitor also induced NOS activity in soybean tissues proximal to the site of inoculation. The induced NOS activity was Ca<sup>2+</sup>- and NADPH-dependent and was sensitive to the NOS inhibitors N<sup><italic>G</italic></sup>-nitro-<sc>l</sc>-arginine methyl ester, aminoguanidine, and <sc>l</sc>-N<sup>6</sup>-(iminoethyl) lysine. NOS activity was not observed in SNP-elicited tissues. An antibody to brain NOS labeled a 166-kD protein in elicited and nonelicited cotyledons. Isoflavones (daidzein and genistein), pterocarpans (glyceollins), and flavones (apigenin and luteolin) were identified after exposure to the elicitor or SNP, although the accumulation of glyceollins and apigenin was limited in SNP-elicited compared with fungal-elicited cotyledons. NOS activity preceded the accumulation of these flavonoids in tissues treated with the Dpm elicitor. The accumulation of these metabolites was faster in SNP-elicited than in fungal-elicited cotyledons. We conclude that the response of soybean cotyledons to Dpm elicitor involves NO formation via a constitutive NOS-like enzyme that triggers the biosynthesis of antimicrobial flavonoids.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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