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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antihyperlipidemic effects of <italic>Citrus sinensis</italic>, <italic>Citrus paradisi</italic>, and their combinations</title><author><name sortKey="Mallick, Neelam" sort="Mallick, Neelam" uniqKey="Mallick N" first="Neelam" last="Mallick">Neelam Mallick</name><affiliation><nlm:aff id="aff1">Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan</nlm:aff></affiliation></author><author><name sortKey="Khan, Rafeeq Alam" sort="Khan, Rafeeq Alam" uniqKey="Khan R" first="Rafeeq Alam" last="Khan">Rafeeq Alam Khan</name><affiliation><nlm:aff id="aff1">Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27134462</idno><idno type="pmc">4832900</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4832900</idno><idno type="RBID">PMC:4832900</idno><idno type="doi">10.4103/0975-7406.171727</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000358</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Antihyperlipidemic effects of <italic>Citrus sinensis</italic>, <italic>Citrus paradisi</italic>, and their combinations</title><author><name sortKey="Mallick, Neelam" sort="Mallick, Neelam" uniqKey="Mallick N" first="Neelam" last="Mallick">Neelam Mallick</name><affiliation><nlm:aff id="aff1">Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan</nlm:aff></affiliation></author><author><name sortKey="Khan, Rafeeq Alam" sort="Khan, Rafeeq Alam" uniqKey="Khan R" first="Rafeeq Alam" last="Khan">Rafeeq Alam Khan</name><affiliation><nlm:aff id="aff1">Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Pharmacy & Bioallied Sciences</title><idno type="ISSN">0976-4879</idno><idno type="eISSN">0975-7406</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Objective:</title><p>Hyperlipidemia, extensively contributes in the progression of coronary heart diseases and atherosclerosis, but may be managed through alterations in the nutritional pattern. Several studies show that diet rich in polyphenols and antioxidants have antiatherogenic effects. <italic>Citrus sinensis</italic> and <italic>Citrus paradisi</italic> are widely known for health benefits and have found to produce antioxidant, anti-inflammatory, and hypolipidemic effects, hence current research was planned to determine the hypolipidemic effects of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> in rats receiving diet rich in cholesterol.</p></sec><sec id="st2"><title>Materials and Methods:</title><p>All rats were divided into 11 groups each comprising 10 animals: Normal control group and hyperlipidemic control. <italic>C. sinensis</italic> treated three groups, <italic>C. paradisi</italic> treated three groups, <italic>C. sinensis</italic> and <italic>C. paradisi</italic> combination treated two groups, and group treated atorvastatin. All rats in the respective groups were treated orally with sterile water, juices, and standard drug for 8 weeks and lipid profile was estimated at the end of dosing.</p></sec><sec id="st3"><title>Results:</title><p>Cholesterol, triglycerides (TGs), and low-density lipoprotein (LDL) were decreased at all the three doses of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> but rise in high-density lipoprotein (HDL) was only significant at 8 ml/kg, and 0.3 ml/kg, respectively. Animals received the combination doses of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> also showed a highly significant fall in cholesterol, LDL, and TGs, however HDL level was significantly elevated by SPJ-2 combination.</p></sec><sec id="st4"><title>Conclusion:</title><p>Results suggest that <italic>C. sinensis</italic> and <italic>C. paradisi</italic> possess antihyperlipidemic activity due to phytochemicals and other essential nutrients, hence may serve as cardioprotective by preventing thrombosis.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Kanakavalli, K" uniqKey="Kanakavalli K">K Kanakavalli</name></author><author><name sortKey="Thillaivanan, S" uniqKey="Thillaivanan S">S Thillaivanan</name></author><author><name sortKey="Parthiban, P" uniqKey="Parthiban P">P Parthiban</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Wald, Nj" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Pharm Bioallied Sci</journal-id><journal-id journal-id-type="iso-abbrev">J Pharm Bioallied Sci</journal-id><journal-id journal-id-type="publisher-id">JPBS</journal-id><journal-title-group><journal-title>Journal of Pharmacy & Bioallied Sciences</journal-title></journal-title-group><issn pub-type="ppub">0976-4879</issn><issn pub-type="epub">0975-7406</issn><publisher><publisher-name>Medknow Publications & Media Pvt Ltd</publisher-name><publisher-loc>India</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27134462</article-id><article-id pub-id-type="pmc">4832900</article-id><article-id pub-id-type="publisher-id">JPBS-8-112</article-id><article-id pub-id-type="doi">10.4103/0975-7406.171727</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Antihyperlipidemic effects of <italic>Citrus sinensis</italic>, <italic>Citrus paradisi</italic>, and their combinations</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mallick</surname><given-names>Neelam</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Khan</surname><given-names>Rafeeq Alam</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"></xref></contrib></contrib-group><aff id="aff1">Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi 75270, Pakistan</aff><author-notes><corresp id="cor1"><bold>Address for correspondence:</bold> Prof. Rafeeq Alam Khan, E-mail: <email xlink:href="rkhan1959@gmail.com">rkhan1959@gmail.com</email></corresp></author-notes><pub-date pub-type="ppub"><season>Apr-Jun</season><year>2016</year></pub-date><volume>8</volume><issue>2</issue><fpage>112</fpage><lpage>118</lpage><history><date date-type="received"><day>16</day><month>6</month><year>2015</year></date><date date-type="rev-recd"><day>06</day><month>8</month><year>2015</year></date><date date-type="accepted"><day>09</day><month>8</month><year>2015</year></date></history><permissions><copyright-statement>Copyright: © Journal of Pharmacy and Bioallied Sciences</copyright-statement><copyright-year>2016</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.</license-p></license></permissions><abstract><sec id="st1"><title>Objective:</title><p>Hyperlipidemia, extensively contributes in the progression of coronary heart diseases and atherosclerosis, but may be managed through alterations in the nutritional pattern. Several studies show that diet rich in polyphenols and antioxidants have antiatherogenic effects. <italic>Citrus sinensis</italic> and <italic>Citrus paradisi</italic> are widely known for health benefits and have found to produce antioxidant, anti-inflammatory, and hypolipidemic effects, hence current research was planned to determine the hypolipidemic effects of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> in rats receiving diet rich in cholesterol.</p></sec><sec id="st2"><title>Materials and Methods:</title><p>All rats were divided into 11 groups each comprising 10 animals: Normal control group and hyperlipidemic control. <italic>C. sinensis</italic> treated three groups, <italic>C. paradisi</italic> treated three groups, <italic>C. sinensis</italic> and <italic>C. paradisi</italic> combination treated two groups, and group treated atorvastatin. All rats in the respective groups were treated orally with sterile water, juices, and standard drug for 8 weeks and lipid profile was estimated at the end of dosing.</p></sec><sec id="st3"><title>Results:</title><p>Cholesterol, triglycerides (TGs), and low-density lipoprotein (LDL) were decreased at all the three doses of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> but rise in high-density lipoprotein (HDL) was only significant at 8 ml/kg, and 0.3 ml/kg, respectively. Animals received the combination doses of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> also showed a highly significant fall in cholesterol, LDL, and TGs, however HDL level was significantly elevated by SPJ-2 combination.</p></sec><sec id="st4"><title>Conclusion:</title><p>Results suggest that <italic>C. sinensis</italic> and <italic>C. paradisi</italic> possess antihyperlipidemic activity due to phytochemicals and other essential nutrients, hence may serve as cardioprotective by preventing thrombosis.</p></sec></abstract><kwd-group><title>KEY WORDS</title><kwd>Grapefruit</kwd><kwd>hyperlipidemia</kwd><kwd>hypocholesterolemic</kwd><kwd>orange</kwd></kwd-group></article-meta></front><body><p>Hyperlipidemia is a diverse group of disorder categorized by the increased levels of lipids and lipoproteins in the bloodstream, e.g., cholesterol, low-density lipoprotein (LDL), and very LDL (VLDL), which eventually leads to atherosclerosis.</p><p>Atherosclerosis and coronary heart diseases (CHD) are the most persistent reasons of illness and death all over the world, though number of causes such as diet rich in saturated fatty acid, high blood pressure, family history, age, and life pattern has an important role in causing ischemic heart disease. However, the increased levels of cholesterol, primarily total cholesterol (TC) and LDL are responsible for the onset of CHDs. A 20% decrease in the cholesterol level can decrease about 31% incidence of CHD and 33% of death rate[<xref rid="ref1" ref-type="bibr">1</xref>] and cardiovascular disorders are the primary cause of death in the western countries.[<xref rid="ref2" ref-type="bibr">2</xref>] Hypercholesterolemia is the main cause of cardiovascular disease (CVD)[<xref rid="ref3" ref-type="bibr">3</xref>] and cellular cholesterol homeostasis is of vital importance in preventing CVD. The plasma cholesterol level can be controlled in numerous ways, e.g., decrease the absorption of dietary lipids, increase the elimination of cholesterol through fecal excretion, decrease cholesterol biosynthesis, and the removal of cholesterol from circulation. There are many studies, which show the valuable effects of HMG-CoA reductase inhibitors and acyl-CoA: Cholesterol acyl transferase (ACAT) inhibitors on hypercholesterolemia and atherosclerosis.[<xref rid="ref4" ref-type="bibr">4</xref><xref rid="ref5" ref-type="bibr">5</xref>] However, existing lipid-lowering drugs have several adverse effects.[<xref rid="ref1" ref-type="bibr">1</xref>]</p><p>In the earlier days, extensive attention has been given to develop awareness about the bioactive constituents of plants and their benefits to health. Human diet from the herbal source comprises several compounds called as nutraceuticals, which though not regarded as nutrients but have an essential part in preserving health; some remarkable nutraceuticals are polyphenols, phytoestrogens, phytates, and polyunsaturated fatty acids.</p><p>The beneficial role of dietary polyphenol such as flavonoids in citrus fruit is the entity of rising scientific interest in human health. Naringin, a naturally occurring bioflavonoid in citrus fruits, has been reported to possess antimicrobial,[<xref rid="ref6" ref-type="bibr">6</xref>] antimutagenic,[<xref rid="ref7" ref-type="bibr">7</xref>] anticarcinogenic,[<xref rid="ref8" ref-type="bibr">8</xref>] and anti-inflammatory[<xref rid="ref9" ref-type="bibr">9</xref>] activities. The effective character of naringin has occupied significant consideration for its use as lipid-lowering and antiatherogenic agent.</p><p>Recent researchers have already explored the lipid-lowering property of naringin, intervened by inhibiting HMG-CoA reductase in rats[<xref rid="ref10" ref-type="bibr">10</xref><xref rid="ref11" ref-type="bibr">11</xref>] but selection of the animal model is significant to test the efficacy of nutraceutical in avoidance of specific disease. Hence, the current research investigates the probability of this functional compound in suppressing diet-induced hypercholesterolemia as efficiently as atorvastatin in rats that prone to develop hypercholesterolemia and atherosclerosis. Antihypercholesterolemic property of bioflavonoids had shown to alter plasma and tissue lipids, cholesterol-regulating enzymes, and tissue morphology in rabbits.[<xref rid="ref12" ref-type="bibr">12</xref>]</p><p>The grapefruit has become famous for health benefits since late 19<sup>th</sup> century but formerly it was only cultivated as an ornamental plant. Grapefruit (<italic>Citrus paradisi</italic>) and sweet orange (<italic>Citrus sinensis</italic>) are the members of the <italic>Rutaceae</italic> family, their juice and pulp parts have shown hypolipidemic effects in the diet-induced hypercholesterolemia in rats. Grapefruit is a good source of Vitamin C, along with dietary fiber, Vitamin A, potassium, folate, and Vitamin B<sub>5</sub>. It also contains phytochemicals, including limonoids and lycopene.[<xref rid="ref13" ref-type="bibr">13</xref>] People developed an interest in grapefruit because of its lipid-lowering abilities, which may be due to the high pectin contents of grapefruit, a soluble fiber that lowers blood cholesterol.[<xref rid="ref14" ref-type="bibr">14</xref>]</p><p>Fujioka <italic>et al</italic>.,[<xref rid="ref15" ref-type="bibr">15</xref>] in a pilot study showed that on average, participants eating half a grapefruit with each meal reduce about 3.6 pounds body weight and those drinking a serving of grapefruit juice 3 times a day lost 3.3 pounds. Moreover, several patients in the study lost more than 10 pounds. Grape fruit is not only a good source of Vitamin C and minerals but also contains protective chemicals, e.g., phenolic acid, limonoids, terpenes, monoterpenes, and bioflavonoids, effective against cancer and heart disease.</p><p>The chief bioflavonoid in grapefruit is Naringin[<xref rid="ref16" ref-type="bibr">16</xref><xref rid="ref17" ref-type="bibr">17</xref>] which gives grapefruit juice bitter taste. Naringin has diverse pharmacological properties such as antioxidant, lipid lowering,[<xref rid="ref18" ref-type="bibr">18</xref>] anticarcinogenic,[<xref rid="ref17" ref-type="bibr">17</xref>] and enzyme inhibition, e.g. CYP3A4 and CYP1A2, responsible for several drug interactions <italic>in vitro</italic>.[<xref rid="ref19" ref-type="bibr">19</xref>] Pink and red grapefruits are rich in lycopene,[<xref rid="ref17" ref-type="bibr">17</xref><xref rid="ref19" ref-type="bibr">19</xref>] an antioxidant that seems to lessen the threat of prostate cancer.[<xref rid="ref20" ref-type="bibr">20</xref>] Grapefruit has also shown relief in some people with rheumatoid arthritis[<xref rid="ref21" ref-type="bibr">21</xref>] and other inflammatory disorders, which might be due to the presence of chemicals that block prostaglandins.</p><p>Orange being a rich source of nutrients has been known for a number of health and nutritional benefits; it contains nearly two hundred phytonutrients and flavonoids, which have shown activity against different types of cancers. They have also been reported to show strong anti-inflammatory and anti-oxidant properties and prevent bone loss.[<xref rid="ref19" ref-type="bibr">19</xref><xref rid="ref22" ref-type="bibr">22</xref><xref rid="ref23" ref-type="bibr">23</xref><xref rid="ref24" ref-type="bibr">24</xref>] The essential oils present in orange juice (<italic>C. sinensis</italic>) are composed of many constituents, including monoterpenes and sesquiterpenes with d-limonene as a major constituent.[<xref rid="ref25" ref-type="bibr">25</xref>] Orange juice or its flavonoid has been reported to produce cholesterol-lowering effect in rabbits[<xref rid="ref26" ref-type="bibr">26</xref>] and humans.[<xref rid="ref27" ref-type="bibr">27</xref>] Its anti-inflammatory role in different disease conditions is also well documented.[<xref rid="ref28" ref-type="bibr">28</xref><xref rid="ref29" ref-type="bibr">29</xref>] Narirutin or Naringenin 7-O-rutinoside is another important flavonoid present abundantly in orange juice.[<xref rid="ref30" ref-type="bibr">30</xref>] It is absorbed well and shows good bioavailability.[<xref rid="ref31" ref-type="bibr">31</xref>] It has shown anti-inflammatory,[<xref rid="ref32" ref-type="bibr">32</xref><xref rid="ref33" ref-type="bibr">33</xref>] anti-allergic, and anti-asthmatic properties.[<xref rid="ref33" ref-type="bibr">33</xref><xref rid="ref34" ref-type="bibr">34</xref>] Orange and grape fruit, both are rich in flavonoids and vitamins, with strong anti-inflammatory and antioxidant properties.[<xref rid="ref19" ref-type="bibr">19</xref><xref rid="ref22" ref-type="bibr">22</xref><xref rid="ref23" ref-type="bibr">23</xref><xref rid="ref24" ref-type="bibr">24</xref>]</p><p>Hence, considering the reported biological activities, a recent study was designed to explore the antihyperlipidemic effect of <italic>C. paradisi</italic>, <italic>C. sinensis</italic>, and their combinations in animals kept on high cholesterol diet.</p><sec sec-type="materials|methods" id="sec1-1"><title>Materials and Methods</title><sec id="sec2-1"><title>Animals</title><p>Adult male Wister rats with mean body weight of 220 ± 10 g were selected for the study and kept under controlled temperature at 23°C ± 2°C and humidity of 50–60%. Five rats were housed in each plastic cage having a size of 81 cm × 46 cm × 41 cm. Rats were maintained on a 12/12 h light and dark cycle during the experiment with nonstop access to rat chow and tap water. The use of animals in the study was in accordance to the guidelines of National Research Council for the care and use of Laboratory Animals[<xref rid="ref35" ref-type="bibr">35</xref>] and permitted by the Board of Advance Studies and Research University of Karachi.</p></sec><sec id="sec2-2"><title>Dosing</title><sec id="sec3-1"><title>Citrus sinensis juice</title><p>The commonly available variety of orange (<italic>C. sinensis</italic>) family <italic>Rutaceae</italic> was purchased from the local vendors. The fruit was recognized by Prof. Anjum Parveen Director, Plant Conservation Center, University of Karachi and specimen sample no C. S 10-10 was submitted to the Department of Pharmacognosy, University of Karachi. Fresh juice was squeezed after peeling the fruit and was used promptly after filtration. <italic>C. sinensis</italic> juice was administered orally in different doses, that is, 2, 5, and 8 ml/kg as per the body weight.</p></sec><sec id="sec3-2"><title>Citrus paradisi juice</title><p>The commonly available variety of grapefruit (<italic>C. paradisi</italic>) family <italic>Rutaceae</italic> was also purchased from the local vendors. The fruit was recognized by Prof. Anjum Parveen Director, Plant Conservation Center; University of Karachi and specimen sample no C.P 09-10 was submitted to the Department of Pharmacognosy, University of Karachi. Fresh juice was squeezed after peeling the fruit, which was used promptly after filtration. <italic>C. paradisi</italic> juice was given orally in three doses, that is, 0.1, 0.3, and 0.5 ml/kg according to the body weight.</p></sec></sec><sec id="sec2-3"><title>Combinations of <italic>Citrus sinensis</italic> and <italic>Citrus paradisi</italic></title><p><italic>C. sinensis</italic> and <italic>C. paradisi</italic> juices were given in combination by mouth in two doses, that is, 2 ml/kg +0.1 ml/kg and 5 ml/kg +0.3 ml/kg, respectively, and were abbreviated as SPJ-1 (2 + 0.1 ml/kg) and SPJ-2 (5 + 0.3 ml/kg), respectively.</p></sec><sec id="sec2-4"><title>Induction of hyperlipidemia</title><p>Hyperlipidemia was induced as described by Sumbul and Ahmed,[<xref rid="ref36" ref-type="bibr">36</xref>] by the addition of egg yolk (24%) of the whole feed of all rats, that is, hyperlipidemic control, test animals, and animals treated with standard drug for a period of 8 weeks.</p></sec><sec id="sec2-5"><title>Design of experiment</title><p>All rats were divided into 11 groups each comprising 10 animals; normal control group was given sterile water with regular diet, hyperlipidemic control group was given sterile water with high cholesterol diet, animals of test group were divided into eight groups; three groups received high cholesterol diet and different doses of <italic>C. sinensis</italic>, other three groups were given high cholesterol diet and <italic>C. paradisi</italic> in different doses, while remaining two groups received high cholesterol diet and combination of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> juices. The animals of the standard group received high cholesterol diet and atorvastatin in the dose of 10 ml/kg.[<xref rid="ref37" ref-type="bibr">37</xref>] All drugs were administered orally on once a daily basis for 8 weeks. At the end of the investigational period, animals were deprived of food overnight and sacrificed by decapitation and blood samples were collected in gel tubes.</p></sec><sec id="sec2-6"><title>Lipid profile</title><p>Serum of blood collected in gel tubes was separated by HuMax 14K centrifuge at 2000 rpm for 10 min and lipid profile was estimated within 3 h on Humalyzer 3000, semi-automatic chemistry analyzer, model number 16700 (Human Germany), with the help of standard kits from Human, Germany.</p></sec><sec id="sec2-7"><title>Cholesterol</title><p>Cholesterol in the serum was determined by CHOD-PAP method, enzymatic colorimetric test for cholesterol with Lipid Clearing Factor. 10 µl sample into cuvette was mixed with 1000 µl enzyme reagent and incubated for 5 min, and then the absorbance was measured within 60 min.[<xref rid="ref38" ref-type="bibr">38</xref>]</p></sec><sec id="sec2-8"><title>Triglycerides</title><p>Triglycerides (TGs) were determined by GPO-PAP method, an enzymatic colorimetric test. 10 µl sample was mixed with 1000 µl mono-reagent in a cuvette and incubated for 10 min, then the absorbance was measured within 60 min.[<xref rid="ref38" ref-type="bibr">38</xref>]</p></sec><sec id="sec2-9"><title>High-density lipoprotein-cholesterol</title><p>The majority of laboratories use several methods for careful precipitation and removal of VLDL and LDL, followed by the enzymatic measurement of high-density lipoprotein (HDL) in the supernatant fraction.[<xref rid="ref39" ref-type="bibr">39</xref>] In this test, HDL-cholesterol, precipitant, and standard was used with human cholesterol Test Kit. 200 µl sample was mixed with 100 µl distilled water and 400 µl PREC HDL reagent, then incubated for 10 min and centrifuged at 3000 rpm for 10 min. 100 µl clear supernatant was taken in a separate tube having 1000 µl cholesterol reagent and the absorbance was measured within 60 min.[<xref rid="ref38" ref-type="bibr">38</xref>]</p></sec><sec id="sec2-10"><title>Low-density lipoprotein-cholesterol</title><p>The LDL-cholesterol was measured by utilizing test kit of Human, Germany. All reagents were warmed at 37°C then pipette 10 µl sample + 750 µl enzyme into the cuvette. Mixed gently and incubated for 5 min, 250 µl substrate was added and incubated at 37°C, and then the absorbance was measured after 5 min.[<xref rid="ref40" ref-type="bibr">40</xref><xref rid="ref41" ref-type="bibr">41</xref>]</p></sec><sec id="sec2-11"><title>Histopathological examination</title><p>Specimens of the liver after removal from the body were preserved in 10% buffered formalin for at least 24 h before further processing. The blocks of organs in cassettes were processed in an automatic tissue processor (Gilford 101 system). The tissue sections were mounted on slides and dried gently in the oven at 60°C for 1 h. This was followed by standard staining procedure for histopathological examination. The slides were then finally examined under a light microscope for morphological changes and photographed by the camera mounted over the microscope for a permanent record.</p></sec><sec id="sec2-12"><title>Statistical analysis</title><p>Data entry and analysis were performed using Superior Performance Statistical Software (SPSS) version 17 IBM 2009. Data were presented as mean ± standard error of the mean with 95% confidence interval. ANOVA followed by <italic>post-hoc</italic> was performed for the comparisons of values with control. Values of <italic>P</italic> ≤ 0.05 were considered as significant and <italic>P</italic> ≤ 0.005 as highly significant.</p></sec></sec><sec sec-type="results" id="sec1-2"><title>Results</title><p><xref ref-type="table" rid="T1">Table 1</xref> displays the result of <italic>C. sinensis</italic> and atorvastatin on cholesterol, LDL, HDL, and TG in hyperlipidemia-induced rats and normal control animals. There was a highly significant decline in cholesterol, LDL, and TG levels by <italic>C. sinensis</italic> at all three doses, that is, 2, 5, and 8 ml/kg in comparison to hyperlipidemic controls, which were similar to the standard drug atorvastatin. However, HDL levels were only significantly increased at 8 ml/kg dose of <italic>C. sinensis</italic>, almost comparable to atorvastatin.</p><table-wrap id="T1" position="float"><label>Table 1</label><caption><p>Effect of <italic>C. sinensis</italic> on lipid profile in rats</p></caption><graphic xlink:href="JPBS-8-112-g001"></graphic></table-wrap><p><xref ref-type="table" rid="T2">Table 2</xref> shows the results of <italic>C. paradisi</italic> on cholesterol, LDL, HDL, and TG in hyperlipidemia-induced rats and normal control rats. There was a highly significant decrease in cholesterol, LDL, and TG level at 0.1, 0.3, and 0.5 ml/kg dose of <italic>C. paradisi</italic> in comparison to hyperlipidemic controls, which were similar to the standard drug atorvastatin. However, a significant increase in HDL level was only observed at 0.3 ml/kg dose of <italic>C. paradisi</italic>, almost similar to the effect of atorvastatin.</p><table-wrap id="T2" position="float"><label>Table 2</label><caption><p>Effect of <italic>C. paradisi</italic> on lipid profile in rats</p></caption><graphic xlink:href="JPBS-8-112-g002"></graphic></table-wrap><p><xref ref-type="table" rid="T3">Table 3</xref> reveals the results of two combination doses of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> on cholesterol, LDL, HDL, and TG in hyperlipidemia-induced and normal control rats. There was a highly significant decrease in cholesterol, LDL, and TG level at SPJ-1 and SPJ-2 in comparison to hyperlipidemic control. This effect was almost similar to the standard drug atorvastatin; however HDL was only significantly increased at SPJ-2.</p><table-wrap id="T3" position="float"><label>Table 3</label><caption><p>Impact of the two doses of combined <italic>C. sinensis</italic> and <italic>C. paradisi</italic> on lipid profile in rats</p></caption><graphic xlink:href="JPBS-8-112-g003"></graphic></table-wrap><sec id="sec2-13"><title>Histopathological examination</title><p>Histopathological examination of hepatic tissue in hyperlipidemia-induced control rats shows hepatocytes surrounded by lipid content with no cytoplasm [<xref ref-type="fig" rid="F1">Figure 1</xref>]. The histopathological examination of hepatic tissue of animals received 0.1 ml/kg of <italic>C. paradisi</italic> shows macrovascular and microvascular steatosis with slight lipid deposition [<xref ref-type="fig" rid="F2">Figure 2</xref>]. However, hepatic tissue of animals received 0.3 ml/kg and 0.5 ml/kg of <italic>C. paradisi</italic>, three doses of <italic>C. sinensis</italic>, and two combination doses of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> did not reveal any microscopic changes in hepatic tissue [Figures <xref ref-type="fig" rid="F3">3</xref> and <xref ref-type="fig" rid="F4">4</xref>].</p><fig id="F1" position="float"><label>Figure 1</label><caption><p>Hepatic tissue in hyperlipidemia-induced control rats showing hepatocytes surrounded by lipid content</p></caption><graphic xlink:href="JPBS-8-112-g004"></graphic></fig><fig id="F2" position="float"><label>Figure 2</label><caption><p>Hepatic tissue in hyperlipidemia-induced rats treated with 0.1 ml/kg of <italic>C. paradisi</italic> showing minor lipid contents</p></caption><graphic xlink:href="JPBS-8-112-g005"></graphic></fig><fig id="F3" position="float"><label>Figure 3</label><caption><p>Hepatic tissues showing no lipid contents in animals that received 0.3 and 0.5 ml/kg of <italic>C. paradisi</italic></p></caption><graphic xlink:href="JPBS-8-112-g006"></graphic></fig><fig id="F4" position="float"><label>Figure 4</label><caption><p>Hepatic tissues showing no lipid contents in animals that received combination doses of <italic>C. paradisi</italic> and <italic>C. sinensis</italic></p></caption><graphic xlink:href="JPBS-8-112-g007"></graphic></fig></sec></sec><sec sec-type="discussion" id="sec1-3"><title>Discussion</title><p>Citrus flavonoids are a group of naturally occurring polyphenols in fruits and vegetables and have been reported to show a variety of biological effects[<xref rid="ref42" ref-type="bibr">42</xref>] along with anti-hypercholesterolemic action,[<xref rid="ref43" ref-type="bibr">43</xref><xref rid="ref44" ref-type="bibr">44</xref>] several <italic>in vivo</italic> studies have already verified the hypocholesterolemic action of naringin in rats.</p><p>Hyperlipidemia is an established risk factor for CVD, particularly atherosclerosis. Coronary artery disease (CAD) is one of the chief reasons for premature death worldwide and is likely to be the most significant cause of death.[<xref rid="ref45" ref-type="bibr">45</xref>] It is well documented that imbalance diet is an important cause of hyperlipidemias and atherosclerosis. Many animal and human studies have established the hypercholesterolemic properties of saturated fatty acids raising TC and changing lipoprotein pattern, however, mechanisms remain under study.</p><p>Cholesterol feeding has been often used to raise serum or tissue cholesterol levels to evaluate the hypercholesterolemia associated metabolic disturbances in different animal models.[<xref rid="ref46" ref-type="bibr">46</xref>] Results of the present study show that keeping the animal on high cholesterol diet significantly increased the TC, TG, and LDL levels in serum as related to rats on a normal diet. However, animals that received high cholesterol diet with <italic>C. sinensis</italic> and <italic>C. paradisi</italic> juices showed considerable decline in the levels of TC, TG, and LDL, however, there was a significant promotion in plasma HDL levels, thus indicating the efficacy of juices in preventing atherosclerotic CAD.</p><p>Sufficient evidence is present with respect to the fact that HDL-cholesterol is inversely related to total body cholesterol and a decrease of plasma HDL-cholesterol level may accelerate the growth of atherosclerosis leading to ischemic heart diseases.[<xref rid="ref47" ref-type="bibr">47</xref>] Flavonoids are described to increase HDL-C concentration and reduce LDL and VLDL levels in the hypercholesterolemic rats.[<xref rid="ref48" ref-type="bibr">48</xref>] Hence, flavonoids and polyphenols found in <italic>C. sinensis</italic> and <italic>C. paradisi</italic> may be considered as promising in elevating HDL and reducing LDL and VLDL in the treated rats.</p><p>Flavonoids and Vitamin C are well documented to cause decrease in LDL and cholesterol level;[<xref rid="ref49" ref-type="bibr">49</xref><xref rid="ref50" ref-type="bibr">50</xref><xref rid="ref51" ref-type="bibr">51</xref><xref rid="ref52" ref-type="bibr">52</xref>] results of the present study showed highly significant reduction in plasma LDL, cholesterol, and TGs level in all the animal groups that received <italic>C. sinensis</italic>, <italic>C. paradisi</italic>, and their combination, this might be due to the high flavonoid content in these fruits. The hypocholesterolemic effect was almost similar to HMG-CoA reductase inhibitor atorvastatin, which was used as a positive control in this study.</p><p>Current research also showed a significant increase in HDL at 8 ml/kg of <italic>C. sinensis</italic>, 0.3 ml/kg of <italic>C. paradisi</italic>, and SPJ-2 combination, which was almost comparable to atorvastatin. This could be justified on the basis of the combined effects of Vitamin C, folate, and flavonoids since polyphenolic antioxidants present in the fruits exhibit a broad range of biological effects,[<xref rid="ref42" ref-type="bibr">42</xref>] including hypocholesterolemic effect.[<xref rid="ref43" ref-type="bibr">43</xref><xref rid="ref44" ref-type="bibr">44</xref>] Naringin is one of the bioflavonoids in citrus and grapefruits that have been reported to have cholesterol lowering and antiatherogenic effect, may be initiated by preventing HMG-CoA reductase activity.[<xref rid="ref10" ref-type="bibr">10</xref><xref rid="ref11" ref-type="bibr">11</xref>]</p><p>ACAT is one of the cholesterol-regulating enzymes that catalyze the esterification of cholesterol with long chain fatty acids and is thought to play a vital role in the progress of atherosclerotic lesions.[<xref rid="ref53" ref-type="bibr">53</xref>] Since the increased hepatic microsomal activity of ACAT is normally associated with high cholesterol diet,[<xref rid="ref25" ref-type="bibr">25</xref><xref rid="ref54" ref-type="bibr">54</xref>] it has been documented that naringin lowers the hepatic microsomal ACAT activity[<xref rid="ref10" ref-type="bibr">10</xref><xref rid="ref50" ref-type="bibr">50</xref>] and it has also been reported that naringin through decreasing the activity of ACAT1 and ACAT2 results in decreased accessibility of lipids for congregation of apoB-consisting lipoproteins and increased plasma HDL-concentration.[<xref rid="ref55" ref-type="bibr">55</xref>] Liver plays an important part in the regulation of plasma LDL and cholesterol metabolism through biliary secretion of bile acids and cholesterol. Naringin may result in significantly higher levels of cholesterol metabolites, levels of cholesterol, and bile acids, which are not absorbed in the small intestine.[<xref rid="ref56" ref-type="bibr">56</xref>] In general, endothelial dysfunction stage in coronary disease is related with total serum cholesterol levels,[<xref rid="ref57" ref-type="bibr">57</xref>] moreover, HDL efficacy to reverse cholesterol transport might be significant for the function of endothelium as indicated in hypercholesterolemic patient that HDL plays a defensive role in endothelial function.[<xref rid="ref58" ref-type="bibr">58</xref>]</p><p>A possible mechanism for lipid lowering effect of citrus flavonoids might be due to its antioxidant effects and due to its inhibition of uptake of oxidized LDL by macrophages, reduced LDL aggregation, and reduced oxidation of LDL cholesterol,[<xref rid="ref59" ref-type="bibr">59</xref>] since macrophages play an important role in the early diagnosis.</p></sec><sec sec-type="conclusion" id="sec1-4"><title>Conclusion</title><p>Result of the recent study shows that juices of <italic>C. sinensis</italic> and <italic>C. paradisi</italic> produced a favorable effect of serum lipid profile in rats, since reduced serum TC, TG, LDL, and increased HDL, thus reducing the chances of atherogenesis. 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