Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Long-Term Boron-Excess-Induced Alterations of Gene Profiles in Roots of Two Citrus Species Differing in Boron-Tolerance Revealed by cDNA-AFLP</title><author><name sortKey="Guo, Peng" sort="Guo, Peng" uniqKey="Guo P" first="Peng" last="Guo">Peng Guo</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name><affiliation><nlm:aff id="aff3"><institution>Institute of Materia Medica, Fujian Academy of Medical Sciences</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Yang, Lin Tong" sort="Yang, Lin Tong" uniqKey="Yang L" first="Lin-Tong" last="Yang">Lin-Tong Yang</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Ye, Xin" sort="Ye, Xin" uniqKey="Ye X" first="Xin" last="Ye">Xin Ye</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Huang, Jing Hao" sort="Huang, Jing Hao" uniqKey="Huang J" first="Jing-Hao" last="Huang">Jing-Hao Huang</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Pomological Institute, Fujian Academy of Agricultural Sciences</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff5"><institution>The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27446128</idno><idno type="pmc">4919357</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919357</idno><idno type="RBID">PMC:4919357</idno><idno type="doi">10.3389/fpls.2016.00898</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000328</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Long-Term Boron-Excess-Induced Alterations of Gene Profiles in Roots of Two Citrus Species Differing in Boron-Tolerance Revealed by cDNA-AFLP</title><author><name sortKey="Guo, Peng" sort="Guo, Peng" uniqKey="Guo P" first="Peng" last="Guo">Peng Guo</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Qi, Yi Ping" sort="Qi, Yi Ping" uniqKey="Qi Y" first="Yi-Ping" last="Qi">Yi-Ping Qi</name><affiliation><nlm:aff id="aff3"><institution>Institute of Materia Medica, Fujian Academy of Medical Sciences</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Yang, Lin Tong" sort="Yang, Lin Tong" uniqKey="Yang L" first="Lin-Tong" last="Yang">Lin-Tong Yang</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Ye, Xin" sort="Ye, Xin" uniqKey="Ye X" first="Xin" last="Ye">Xin Ye</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Huang, Jing Hao" sort="Huang, Jing Hao" uniqKey="Huang J" first="Jing-Hao" last="Huang">Jing-Hao Huang</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Pomological Institute, Fujian Academy of Agricultural Sciences</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author><author><name sortKey="Chen, Li Song" sort="Chen, Li Song" uniqKey="Chen L" first="Li-Song" last="Chen">Li-Song Chen</name><affiliation><nlm:aff id="aff1"><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation><affiliation><nlm:aff id="aff5"><institution>The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></nlm:aff></affiliation></author></analytic><series><title level="j">Frontiers in Plant Science</title><idno type="eISSN">1664-462X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Boron (B) toxicity is observed in some citrus orchards in China. However, limited data are available on the molecular mechanisms of citrus B-toxicity and B-tolerance. Using cDNA-AFLP, we identified 20 up- and 52 down-regulated genes, and 44 up- and 66 down-regulated genes from excess B-treated <italic>Citrus sinensis</italic> and <italic>Citrus grandis</italic> roots, respectively, thereby demonstrating that gene expression profiles were more affected in the latter. In addition, phosphorus and total soluble protein concentrations were lowered only in excess B-treated <italic>C. grandis</italic> roots. Apparently, <italic>C. sinensis</italic> had higher B-tolerance than <italic>C. grandis</italic>. Our results suggested that the following several aspects were responsible for the difference in the B-tolerance between the two citrus species including: (a) B-excess induced <italic>Root Hair Defective 3</italic> expression in <italic>C. sinensis</italic> roots, and repressed <italic>villin4</italic> expression in <italic>C. grandis</italic> roots; accordingly, root growth was less inhibited by B-excess in the former; (b) antioxidant systems were impaired in excess B-treated <italic>C. grandis</italic> roots, hence accelerating root senescence; (c) genes related to Ca<sup>2+</sup> signals were inhibited (induced) by B-excess in <italic>C. grandis</italic> (<italic>C. sinensis</italic>) roots. B-excess-responsive genes related to energy (i.e., <italic>alternative oxidase</italic> and <italic>cytochrome P450</italic>), lipid (i.e., <italic>Glycerol-3-phosphate acyltransferase 9</italic> and <italic>citrus dioxygenase</italic>), and nucleic acid (i.e., <italic>HDA19, histone 4</italic>, and <italic>ribonucleotide reductase RNR1 like protein</italic>) metabolisms also possibly accounted for the difference in the B-tolerance between the two citrus species. These data increased our understanding of the mechanisms on citrus B-toxicity and B-tolerance at transcriptional level.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Ames, B N" uniqKey="Ames B">B. N. Ames</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aquea, F" uniqKey="Aquea F">F. Aquea</name></author><author><name sortKey="Federici, F" uniqKey="Federici F">F. Federici</name></author><author><name sortKey="Moscoso, C" uniqKey="Moscoso C">C. Moscoso</name></author><author><name sortKey="Vega, A" uniqKey="Vega A">A. Vega</name></author><author><name sortKey="Jullian, P" uniqKey="Jullian P">P. Jullian</name></author><author><name sortKey="Haseloff, J" uniqKey="Haseloff J">J. Haseloff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Axelrod, B" uniqKey="Axelrod B">B. Axelrod</name></author><author><name sortKey="Cheesbrough, T M" uniqKey="Cheesbrough T">T. M. Cheesbrough</name></author><author><name sortKey="Laakso, S" uniqKey="Laakso S">S. Laakso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ayvaz, M" uniqKey="Ayvaz M">M. Ayvaz</name></author><author><name sortKey="Guven, A" uniqKey="Guven A">A. Guven</name></author><author><name sortKey="Fagerstedt, K" uniqKey="Fagerstedt K">K. Fagerstedt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ayvaz, M" uniqKey="Ayvaz M">M. Ayvaz</name></author><author><name sortKey="Koyuncu, M" uniqKey="Koyuncu M">M. Koyuncu</name></author><author><name sortKey="Guven, A" uniqKey="Guven A">A. Guven</name></author><author><name sortKey="Fagerstedt, K V" uniqKey="Fagerstedt K">K. V. Fagerstedt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bradford, M M" uniqKey="Bradford M">M. M. Bradford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cervilla, L M" uniqKey="Cervilla L">L. M. Cervilla</name></author><author><name sortKey="Blasco, B" uniqKey="Blasco B">B. Blasco</name></author><author><name sortKey="Rios, J" uniqKey="Rios J">J. Ríos</name></author><author><name sortKey="Romero, L" uniqKey="Romero L">L. Romero</name></author><author><name sortKey="Ruiz, J" uniqKey="Ruiz J">J. Ruiz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cervilla, L M" uniqKey="Cervilla L">L. M. Cervilla</name></author><author><name sortKey="Blasco, B" uniqKey="Blasco B">B. Blasco</name></author><author><name sortKey="Rios, J J" uniqKey="Rios J">J. J. Ríos</name></author><author><name sortKey="Rosales, M A" uniqKey="Rosales M">M. A. Rosales</name></author><author><name sortKey="Rubio Wilhelmi, M M" uniqKey="Rubio Wilhelmi M">M. M. Rubio-Wilhelmi</name></author><author><name sortKey="Sanchez Rodriguez, E" uniqKey="Sanchez Rodriguez E">E. Sánchez-Rodríguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chaboute, M E" uniqKey="Chaboute M">M. E. Chabouté</name></author><author><name sortKey="Combettes, B" uniqKey="Combettes B">B. Combettes</name></author><author><name sortKey="Clement, B" uniqKey="Clement B">B. Clément</name></author><author><name sortKey="Gigot, C" uniqKey="Gigot C">C. Gigot</name></author><author><name sortKey="Philipps, G" uniqKey="Philipps G">G. Philipps</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, C Y" uniqKey="Chen C">C. Y. Chen</name></author><author><name sortKey="Wu, K" uniqKey="Wu K">K. Wu</name></author><author><name sortKey="Schmidt, W" uniqKey="Schmidt W">W. Schmidt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, L S" uniqKey="Chen L">L. S. Chen</name></author><author><name sortKey="Han, S" uniqKey="Han S">S. Han</name></author><author><name sortKey="Qi, Y P" uniqKey="Qi Y">Y. P. Qi</name></author><author><name sortKey="Yang, L T" uniqKey="Yang L">L. T. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, M" uniqKey="Chen M">M. Chen</name></author><author><name sortKey="Mishra, S" uniqKey="Mishra S">S. Mishra</name></author><author><name sortKey="Heckathorn, S A" uniqKey="Heckathorn S">S. A. Heckathorn</name></author><author><name sortKey="Frantz, J M" uniqKey="Frantz J">J. M. Frantz</name></author><author><name sortKey="Krause, C" uniqKey="Krause C">C. Krause</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheung, M Y" uniqKey="Cheung M">M. Y. Cheung</name></author><author><name sortKey="Li, M W" uniqKey="Li M">M. W. Li</name></author><author><name sortKey="Yung, Y L" uniqKey="Yung Y">Y. L. Yung</name></author><author><name sortKey="Wen, C Q" uniqKey="Wen C">C. Q. Wen</name></author><author><name sortKey="Lam, H M" uniqKey="Lam H">H. M. Lam</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collakova, E" uniqKey="Collakova E">E. Collakova</name></author><author><name sortKey="Dellapenna, D" uniqKey="Dellapenna D">D. DellaPenna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Craciun, A R" uniqKey="Craciun A">A. R. Craciun</name></author><author><name sortKey="Courbot, M" uniqKey="Courbot M">M. Courbot</name></author><author><name sortKey="Bourgis, F" uniqKey="Bourgis F">F. Bourgis</name></author><author><name sortKey="Salis, P" uniqKey="Salis P">P. Salis</name></author><author><name sortKey="Saumitou Laprade, P" uniqKey="Saumitou Laprade P">P. Saumitou-Laprade</name></author><author><name sortKey="Verbruggen, N" uniqKey="Verbruggen N">N. Verbruggen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deshmukh, R K" uniqKey="Deshmukh R">R. K. Deshmukh</name></author><author><name sortKey="Vivancos, J" uniqKey="Vivancos J">J. Vivancos</name></author><author><name sortKey="Ramakrishnan, G" uniqKey="Ramakrishnan G">G. Ramakrishnan</name></author><author><name sortKey="Guerin, V" uniqKey="Guerin V">V. Guérin</name></author><author><name sortKey="Carpentier, G" uniqKey="Carpentier G">G. Carpentier</name></author><author><name sortKey="Sonah, H" uniqKey="Sonah H">H. Sonah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ditt, R F" uniqKey="Ditt R">R. F. Ditt</name></author><author><name sortKey="Nester, E W" uniqKey="Nester E">E. W. Nester</name></author><author><name sortKey="Comai, L" uniqKey="Comai L">L. Comai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drury, G E" uniqKey="Drury G">G. E. Drury</name></author><author><name sortKey="Dowle, A A" uniqKey="Dowle A">A. A. Dowle</name></author><author><name sortKey="Ashford, D A" uniqKey="Ashford D">D. A. Ashford</name></author><author><name sortKey="Waterworth, W M" uniqKey="Waterworth W">W. M. Waterworth</name></author><author><name sortKey="Thomas, J" uniqKey="Thomas J">J. Thomas</name></author><author><name sortKey="West, C E" uniqKey="West C">C. E. West</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fang, K" uniqKey="Fang K">K. Fang</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W. Zhang</name></author><author><name sortKey="Xing, Y" uniqKey="Xing Y">Y. Xing</name></author><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q. Zhang</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L. Yang</name></author><author><name sortKey="Cao, Q" uniqKey="Cao Q">Q. Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fusco, N" uniqKey="Fusco N">N. Fusco</name></author><author><name sortKey="Micheletto, L" uniqKey="Micheletto L">L. Micheletto</name></author><author><name sortKey="Dalcorso, G" uniqKey="Dalcorso G">G. DalCorso</name></author><author><name sortKey="Borgato, L" uniqKey="Borgato L">L. Borgato</name></author><author><name sortKey="Furini, A" uniqKey="Furini A">A. Furini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gaedeke, N" uniqKey="Gaedeke N">N. Gaedeke</name></author><author><name sortKey="Klein, M" uniqKey="Klein M">M. Klein</name></author><author><name sortKey="Kolukisaoglu, U" uniqKey="Kolukisaoglu U">U. Kolukisaoglu</name></author><author><name sortKey="Forestier, C" uniqKey="Forestier C">C. Forestier</name></author><author><name sortKey="Muller, A" uniqKey="Muller A">A. Müller</name></author><author><name sortKey="Ansorge, M" uniqKey="Ansorge M">M. Ansorge</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gorinova, N" uniqKey="Gorinova N">N. Gorinova</name></author><author><name sortKey="Nedkovska, M" uniqKey="Nedkovska M">M. Nedkovska</name></author><author><name sortKey="Atanassov, A" uniqKey="Atanassov A">A. Atanassov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gunes, A" uniqKey="Gunes A">A. Gunes</name></author><author><name sortKey="Soylemezoglu, G" uniqKey="Soylemezoglu G">G. Soylemezoglu</name></author><author><name sortKey="Inal, A" uniqKey="Inal A">A. Inal</name></author><author><name sortKey="Bagci, E G" uniqKey="Bagci E">E. G. Bagci</name></author><author><name sortKey="Coban, S" uniqKey="Coban S">S. Coban</name></author><author><name sortKey="Sahin, O" uniqKey="Sahin O">O. Sahin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, P" uniqKey="Guo P">P. Guo</name></author><author><name sortKey="Qi, Y P" uniqKey="Qi Y">Y. P. Qi</name></author><author><name sortKey="Yang, L T" uniqKey="Yang L">L. T. Yang</name></author><author><name sortKey="Ye, X" uniqKey="Ye X">X. Ye</name></author><author><name sortKey="Jiang, H X" uniqKey="Jiang H">H. X. Jiang</name></author><author><name sortKey="Huang, J H" uniqKey="Huang J">J. H. Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Han, S" uniqKey="Han S">S. Han</name></author><author><name sortKey="Tang, N" uniqKey="Tang N">N. Tang</name></author><author><name sortKey="Jiang, H X" uniqKey="Jiang H">H. X. Jiang</name></author><author><name sortKey="Yang, L T" uniqKey="Yang L">L. T. Yang</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Chen, L S" uniqKey="Chen L">L. S. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hassan, M" uniqKey="Hassan M">M. Hassan</name></author><author><name sortKey="Oldach, K" uniqKey="Oldach K">K. Oldach</name></author><author><name sortKey="Baumann, U" uniqKey="Baumann U">U. Baumann</name></author><author><name sortKey="Langridge, P" uniqKey="Langridge P">P. Langridge</name></author><author><name sortKey="Sutton, T" uniqKey="Sutton T">T. Sutton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y. Hu</name></author><author><name sortKey="Zhang, R G" uniqKey="Zhang R">R. G. Zhang</name></author><author><name sortKey="Morriison, W H" uniqKey="Morriison W">W. H. Morriison</name></author><author><name sortKey="Ye, Z H" uniqKey="Ye Z">Z. H. Ye</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, J H" uniqKey="Huang J">J. H. Huang</name></author><author><name sortKey="Cai, Z J" uniqKey="Cai Z">Z. J. Cai</name></author><author><name sortKey="Wen, S X" uniqKey="Wen S">S. X. Wen</name></author><author><name sortKey="Guo, P" uniqKey="Guo P">P. Guo</name></author><author><name sortKey="Ye, X" uniqKey="Ye X">X. Ye</name></author><author><name sortKey="Lin, G Z" uniqKey="Lin G">G. Z. Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, J H" uniqKey="Huang J">J. H. Huang</name></author><author><name sortKey="Qi, Y P" uniqKey="Qi Y">Y. P. Qi</name></author><author><name sortKey="Wen, S X" uniqKey="Wen S">S. X. Wen</name></author><author><name sortKey="Guo, P" uniqKey="Guo P">P. Guo</name></author><author><name sortKey="Chen, X M" uniqKey="Chen X">X. M. Chen</name></author><author><name sortKey="Chen, L S" uniqKey="Chen L">L. S. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Inal, A" uniqKey="Inal A">A. Inal</name></author><author><name sortKey="Pilbeam, D J" uniqKey="Pilbeam D">D. J. Pilbeam</name></author><author><name sortKey="Gunes, A" uniqKey="Gunes A">A. Gunes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jayasekaran, K" uniqKey="Jayasekaran K">K. Jayasekaran</name></author><author><name sortKey="Kim, K N" uniqKey="Kim K">K. N. Kim</name></author><author><name sortKey="Vivekanandan, M" uniqKey="Vivekanandan M">M. Vivekanandan</name></author><author><name sortKey="Shin, J S" uniqKey="Shin J">J. S. Shin</name></author><author><name sortKey="Ok, S H" uniqKey="Ok S">S. H. Ok</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, H X" uniqKey="Jiang H">H. X. Jiang</name></author><author><name sortKey="Tang, N" uniqKey="Tang N">N. Tang</name></author><author><name sortKey="Zheng, J G" uniqKey="Zheng J">J. G. Zheng</name></author><author><name sortKey="Chen, L S" uniqKey="Chen L">L. S. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, H X" uniqKey="Jiang H">H. X. Jiang</name></author><author><name sortKey="Tang, N" uniqKey="Tang N">N. Tang</name></author><author><name sortKey="Zheng, J G" uniqKey="Zheng J">J. G. Zheng</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Chen, L S" uniqKey="Chen L">L. S. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kasajima, I" uniqKey="Kasajima I">I. Kasajima</name></author><author><name sortKey="Fujiwara, T" uniqKey="Fujiwara T">T. Fujiwara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaya, C" uniqKey="Kaya C">C. Kaya</name></author><author><name sortKey="Tuna, A L" uniqKey="Tuna A">A. L. Tuna</name></author><author><name sortKey="Dikilitas, M" uniqKey="Dikilitas M">M. Dikilitas</name></author><author><name sortKey="Ashraf, M" uniqKey="Ashraf M">M. Ashraf</name></author><author><name sortKey="Koskeroglu, S" uniqKey="Koskeroglu S">S. Koskeroglu</name></author><author><name sortKey="Guneri, M" uniqKey="Guneri M">M. Guneri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Keles, Y" uniqKey="Keles Y">Y. Keles</name></author><author><name sortKey="Oncel, I" uniqKey="Oncel I">I. Oncel</name></author><author><name sortKey="Yenice, N" uniqKey="Yenice N">N. Yenice</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, D Y" uniqKey="Kim D">D. Y. Kim</name></author><author><name sortKey="Bovet, L" uniqKey="Bovet L">L. Bovet</name></author><author><name sortKey="Maeshima, M" uniqKey="Maeshima M">M. Maeshima</name></author><author><name sortKey="Martinoia, E" uniqKey="Martinoia E">E. Martinoia</name></author><author><name sortKey="Lee, Y" uniqKey="Lee Y">Y. Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knight, H" uniqKey="Knight H">H. Knight</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q. Li</name></author><author><name sortKey="Chen, L S" uniqKey="Chen L">L. S. Chen</name></author><author><name sortKey="Jiang, H X" uniqKey="Jiang H">H. X. Jiang</name></author><author><name sortKey="Tang, N" uniqKey="Tang N">N. Tang</name></author><author><name sortKey="Yang, L T" uniqKey="Yang L">L. T. Yang</name></author><author><name sortKey="Lin, Z H" uniqKey="Lin Z">Z. H. Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Han, M Q" uniqKey="Han M">M. Q. Han</name></author><author><name sortKey="Lin, F" uniqKey="Lin F">F. Lin</name></author><author><name sortKey="Ten, Y" uniqKey="Ten Y">Y. Ten</name></author><author><name sortKey="Lin, J" uniqKey="Lin J">J. Lin</name></author><author><name sortKey="Zhu, D H" uniqKey="Zhu D">D. H. Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, Y B" uniqKey="Lu Y">Y. B. Lu</name></author><author><name sortKey="Qi, Y P" uniqKey="Qi Y">Y. P. Qi</name></author><author><name sortKey="Lee, J" uniqKey="Lee J">J. Lee</name></author><author><name sortKey="Guo, P" uniqKey="Guo P">P. Guo</name></author><author><name sortKey="Ye, X" uniqKey="Ye X">X. Ye</name></author><author><name sortKey="Jia, M" uniqKey="Jia M">M. Jia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mao, C H" uniqKey="Mao C">C. H. Mao</name></author><author><name sortKey="Yi, K" uniqKey="Yi K">K. Yi</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L. Yang</name></author><author><name sortKey="Zheng, B" uniqKey="Zheng B">B. Zheng</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y. Wu</name></author><author><name sortKey="Liu, F" uniqKey="Liu F">F. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martinez Cuenca, M R" uniqKey="Martinez Cuenca M">M. R. Martínez-Cuenca</name></author><author><name sortKey="Martinez Alcantara, B" uniqKey="Martinez Alcantara B">B. Martínez-Alcántara</name></author><author><name sortKey="Qui Ones, A" uniqKey="Qui Ones A">A. Quiñones</name></author><author><name sortKey="Ruiz, M" uniqKey="Ruiz M">M. Ruiz</name></author><author><name sortKey="Iglesias, D J" uniqKey="Iglesias D">D. J. Iglesias</name></author><author><name sortKey="Primo Millo, E" uniqKey="Primo Millo E">E. Primo-Millo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maxwell, D P" uniqKey="Maxwell D">D. P. Maxwell</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Mcintosh, L" uniqKey="Mcintosh L">L. McIntosh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mesquita, G L" uniqKey="Mesquita G">G. L. Mesquita</name></author><author><name sortKey="Zambrosi, F C B" uniqKey="Zambrosi F">F. C. B. Zambrosi</name></author><author><name sortKey="Tanaka, F A O" uniqKey="Tanaka F">F. A. O. Tanaka</name></author><author><name sortKey="Boaretto, R M" uniqKey="Boaretto R">R. M. Boaretto</name></author><author><name sortKey="Quaggio, J A" uniqKey="Quaggio J">J. A. Quaggio</name></author><author><name sortKey="Ribeiro, R V" uniqKey="Ribeiro R">R. V. Ribeiro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nable, R O" uniqKey="Nable R">R. O. Nable</name></author><author><name sortKey="Banuelos, G S" uniqKey="Banuelos G">G. S. Banuelos</name></author><author><name sortKey="Paull, J G" uniqKey="Paull J">J. G. Paull</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Padmanabhan, P" uniqKey="Padmanabhan P">P. Padmanabhan</name></author><author><name sortKey="Babao Lu, M" uniqKey="Babao Lu M">M. Babaoğlu</name></author><author><name sortKey="Terry, N" uniqKey="Terry N">N. Terry</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pandey, N" uniqKey="Pandey N">N. Pandey</name></author><author><name sortKey="Archana" uniqKey="Archana">Archana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Papadakis, I E" uniqKey="Papadakis I">I. E. Papadakis</name></author><author><name sortKey="Dimassi, K N" uniqKey="Dimassi K">K. N. Dimassi</name></author><author><name sortKey="Bosabalidis, A M" uniqKey="Bosabalidis A">A. M. Bosabalidis</name></author><author><name sortKey="Therios, I N" uniqKey="Therios I">I. N. Therios</name></author><author><name sortKey="Patakas, A" uniqKey="Patakas A">A. Patakas</name></author><author><name sortKey="Giannakoula, A" uniqKey="Giannakoula A">A. Giannakoula</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Papadakis, I E" uniqKey="Papadakis I">I. E. Papadakis</name></author><author><name sortKey="Dimassi, K N" uniqKey="Dimassi K">K. N. Dimassi</name></author><author><name sortKey="Bosabalidis, A M" uniqKey="Bosabalidis A">A. M. Bosabalidis</name></author><author><name sortKey="Therios, I N" uniqKey="Therios I">I. N. Therios</name></author><author><name sortKey="Patakas, A" uniqKey="Patakas A">A. Patakas</name></author><author><name sortKey="Giannakoula, A" uniqKey="Giannakoula A">A. Giannakoula</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Papadakis, I E" uniqKey="Papadakis I">I. E. Papadakis</name></author><author><name sortKey="Dimassi, K N" uniqKey="Dimassi K">K. N. Dimassi</name></author><author><name sortKey="Therios, I N" uniqKey="Therios I">I. N. Therios</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paun, O" uniqKey="Paun O">O. Paun</name></author><author><name sortKey="Schonswetter, P" uniqKey="Schonswetter P">P. Schönswetter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perez Gilabert, M" uniqKey="Perez Gilabert M">M. Pérez-Gilabert</name></author><author><name sortKey="L Pez Nicolas, J M" uniqKey="L Pez Nicolas J">J. M. López-Nicolás</name></author><author><name sortKey="Carmona, F G" uniqKey="Carmona F">F. G. Carmona</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Popov, V N" uniqKey="Popov V">V. N. Popov</name></author><author><name sortKey="Simonian, R A" uniqKey="Simonian R">R. A. Simonian</name></author><author><name sortKey="Skulachev, V P" uniqKey="Skulachev V">V. P. Skulachev</name></author><author><name sortKey="Starkov, A A" uniqKey="Starkov A">A. A. Starkov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Purvis, A C" uniqKey="Purvis A">A. C. Purvis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reid, R J" uniqKey="Reid R">R. J. Reid</name></author><author><name sortKey="Hayes, J E" uniqKey="Hayes J">J. E. Hayes</name></author><author><name sortKey="Post, A" uniqKey="Post A">A. Post</name></author><author><name sortKey="Stangoulis, J C R" uniqKey="Stangoulis J">J. C. R. Stangoulis</name></author><author><name sortKey="Graham, R D" uniqKey="Graham R">R. D. Graham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sakamoto, T" uniqKey="Sakamoto T">T. Sakamoto</name></author><author><name sortKey="Inui, Y T" uniqKey="Inui Y">Y. T. Inui</name></author><author><name sortKey="Uraguchi, S" uniqKey="Uraguchi S">S. Uraguchi</name></author><author><name sortKey="Yoshizumi, T" uniqKey="Yoshizumi T">T. Yoshizumi</name></author><author><name sortKey="Matsunaga, S" uniqKey="Matsunaga S">S. Matsunaga</name></author><author><name sortKey="Mastui, M" uniqKey="Mastui M">M. Mastui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sang, W" uniqKey="Sang W">W. Sang</name></author><author><name sortKey="Huang, Z R" uniqKey="Huang Z">Z. R. Huang</name></author><author><name sortKey="Qi, Y P" uniqKey="Qi Y">Y. P. Qi</name></author><author><name sortKey="Yang, L T" uniqKey="Yang L">L. T. Yang</name></author><author><name sortKey="Guo, P" uniqKey="Guo P">P. Guo</name></author><author><name sortKey="Chen, L S" uniqKey="Chen L">L. S. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schnurbusch, T" uniqKey="Schnurbusch T">T. Schnurbusch</name></author><author><name sortKey="Hayes, J" uniqKey="Hayes J">J. Hayes</name></author><author><name sortKey="Hrmova, M" uniqKey="Hrmova M">M. Hrmova</name></author><author><name sortKey="Baumann, U" uniqKey="Baumann U">U. Baumann</name></author><author><name sortKey="Ramesh, S A" uniqKey="Ramesh S">S. A. Ramesh</name></author><author><name sortKey="Tyerman, S D" uniqKey="Tyerman S">S. D. Tyerman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sheng, O" uniqKey="Sheng O">O. Sheng</name></author><author><name sortKey="Zhou, G F" uniqKey="Zhou G">G. F. Zhou</name></author><author><name sortKey="Wei, Q J" uniqKey="Wei Q">Q. J. Wei</name></author><author><name sortKey="Peng, S A" uniqKey="Peng S">S. A. Peng</name></author><author><name sortKey="Deng, X X" uniqKey="Deng X">X. X. Deng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shockey, J" uniqKey="Shockey J">J. Shockey</name></author><author><name sortKey="Regmi, A" uniqKey="Regmi A">A. Regmi</name></author><author><name sortKey="Cotton, K" uniqKey="Cotton K">K. Cotton</name></author><author><name sortKey="Adhikari, N" uniqKey="Adhikari N">N. Adhikari</name></author><author><name sortKey="Browse, J" uniqKey="Browse J">J. Browse</name></author><author><name sortKey="Bates, P D" uniqKey="Bates P">P. D. Bates</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Siddiqui, M H" uniqKey="Siddiqui M">M. H. Siddiqui</name></author><author><name sortKey="Al Whaibi, M H" uniqKey="Al Whaibi M">M. H. Al-Whaibi</name></author><author><name sortKey="Sakran, A M" uniqKey="Sakran A">A. M. Sakran</name></author><author><name sortKey="Ali, H M" uniqKey="Ali H">H. M. Ali</name></author><author><name sortKey="Basalah, M O" uniqKey="Basalah M">M. O. Basalah</name></author><author><name sortKey="Faisal, M" uniqKey="Faisal M">M. Faisal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Singh, J P" uniqKey="Singh J">J. P. Singh</name></author><author><name sortKey="Dahiya, D J" uniqKey="Dahiya D">D. J. Dahiya</name></author><author><name sortKey="Narwal, R P" uniqKey="Narwal R">R. P. Narwal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tian, L" uniqKey="Tian L">L. Tian</name></author><author><name sortKey="Chen, Z J" uniqKey="Chen Z">Z. J. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tian, L" uniqKey="Tian L">L. Tian</name></author><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Fong, M P" uniqKey="Fong M">M. P. Fong</name></author><author><name sortKey="Chen, M" uniqKey="Chen M">M. Chen</name></author><author><name sortKey="Cao, H" uniqKey="Cao H">H. Cao</name></author><author><name sortKey="Gelvin, S B" uniqKey="Gelvin S">S. B. Gelvin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tirajoh, A" uniqKey="Tirajoh A">A. Tirajoh</name></author><author><name sortKey="Aung, T S" uniqKey="Aung T">T. S. Aung</name></author><author><name sortKey="Mckay, A B" uniqKey="Mckay A">A. B. McKay</name></author><author><name sortKey="Plant, A L" uniqKey="Plant A">A. L. Plant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tombuloglu, G" uniqKey="Tombuloglu G">G. Tombuloglu</name></author><author><name sortKey="Tombuloglu, H" uniqKey="Tombuloglu H">H. Tombuloglu</name></author><author><name sortKey="Sakcali, M S" uniqKey="Sakcali M">M. S. Sakcali</name></author><author><name sortKey="Unver, T" uniqKey="Unver T">T. Unver</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turan, M A" uniqKey="Turan M">M. A. Turan</name></author><author><name sortKey="Taban, N" uniqKey="Taban N">N. Taban</name></author><author><name sortKey="Taban, S" uniqKey="Taban S">S. Taban</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turner, B M" uniqKey="Turner B">B. M. Turner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vantini, J S" uniqKey="Vantini J">J. S. Vantini</name></author><author><name sortKey="Dedemo, G C" uniqKey="Dedemo G">G. C. Dedemo</name></author><author><name sortKey="Jovino Gimenez, D F" uniqKey="Jovino Gimenez D">D. F. Jovino Gimenez</name></author><author><name sortKey="Fonseca, L F" uniqKey="Fonseca L">L. F. Fonseca</name></author><author><name sortKey="Tezza, R I" uniqKey="Tezza R">R. I. Tezza</name></author><author><name sortKey="Mutton, M A" uniqKey="Mutton M">M. A. Mutton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, C" uniqKey="Wang C">C. Wang</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, H Y" uniqKey="Wang H">H. Y. Wang</name></author><author><name sortKey="Lee, M M" uniqKey="Lee M">M. M. Lee</name></author><author><name sortKey="Schiefelbein, J W" uniqKey="Schiefelbein J">J. W. Schiefelbein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Nakazato, T" uniqKey="Nakazato T">T. Nakazato</name></author><author><name sortKey="Sakanishi, K" uniqKey="Sakanishi K">K. Sakanishi</name></author><author><name sortKey="Yamada, O" uniqKey="Yamada O">O. Yamada</name></author><author><name sortKey="Tao, H" uniqKey="Tao H">H. Tao</name></author><author><name sortKey="Saito, I" uniqKey="Saito I">I. Saito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Z Y" uniqKey="Wang Z">Z. Y. Wang</name></author><author><name sortKey="Shen, K" uniqKey="Shen K">K. Shen</name></author><author><name sortKey="Zhang, F S" uniqKey="Zhang F">F. S. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, M" uniqKey="Xu M">M. Xu</name></author><author><name sortKey="Xie, W" uniqKey="Xie W">W. Xie</name></author><author><name sortKey="Huang, M" uniqKey="Huang M">M. Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, X M" uniqKey="Xu X">X. M. Xu</name></author><author><name sortKey="Lin, H" uniqKey="Lin H">H. Lin</name></author><author><name sortKey="Maple, J" uniqKey="Maple J">J. Maple</name></author><author><name sortKey="Bjorkblom, B" uniqKey="Bjorkblom B">B. Björkblom</name></author><author><name sortKey="Alves, G" uniqKey="Alves G">G. Alves</name></author><author><name sortKey="Larsen, J P" uniqKey="Larsen J">J. P. Larsen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, X M" uniqKey="Xu X">X. M. Xu</name></author><author><name sortKey="M Ller, S G" uniqKey="M Ller S">S. G. Møller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y. Zhang</name></author><author><name sortKey="Xiao, Y" uniqKey="Xiao Y">Y. Xiao</name></author><author><name sortKey="Du, F" uniqKey="Du F">F. Du</name></author><author><name sortKey="Cao, L" uniqKey="Cao L">L. Cao</name></author><author><name sortKey="Dong, H" uniqKey="Dong H">H. Dong</name></author><author><name sortKey="Ren, H" uniqKey="Ren H">H. Ren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, C P" uniqKey="Zhou C">C. P. Zhou</name></author><author><name sortKey="Qi, Y P" uniqKey="Qi Y">Y. P. Qi</name></author><author><name sortKey="You, X" uniqKey="You X">X. You</name></author><author><name sortKey="Yang, L T" uniqKey="Yang L">L. T. Yang</name></author><author><name sortKey="Guo, P" uniqKey="Guo P">P. Guo</name></author><author><name sortKey="Ye, X" uniqKey="Ye X">X. Ye</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y. Zhu</name></author><author><name sortKey="Dong, A" uniqKey="Dong A">A. Dong</name></author><author><name sortKey="Shen, W H" uniqKey="Shen W">W. H. Shen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zidenga, T" uniqKey="Zidenga T">T. Zidenga</name></author><author><name sortKey="Leyva Guerrero, E" uniqKey="Leyva Guerrero E">E. Leyva-Guerrero</name></author><author><name sortKey="Moon, H" uniqKey="Moon H">H. Moon</name></author><author><name sortKey="Siritunga, D" uniqKey="Siritunga D">D. Siritunga</name></author><author><name sortKey="Sayre, R" uniqKey="Sayre R">R. Sayre</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Front Plant Sci</journal-id><journal-id journal-id-type="iso-abbrev">Front Plant Sci</journal-id><journal-id journal-id-type="publisher-id">Front. Plant Sci.</journal-id><journal-title-group><journal-title>Frontiers in Plant Science</journal-title></journal-title-group><issn pub-type="epub">1664-462X</issn><publisher><publisher-name>Frontiers Media S.A.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27446128</article-id><article-id pub-id-type="pmc">4919357</article-id><article-id pub-id-type="doi">10.3389/fpls.2016.00898</article-id><article-categories><subj-group subj-group-type="heading"><subject>Plant Science</subject><subj-group><subject>Original Research</subject></subj-group></subj-group></article-categories><title-group><article-title>Long-Term Boron-Excess-Induced Alterations of Gene Profiles in Roots of Two Citrus Species Differing in Boron-Tolerance Revealed by cDNA-AFLP</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Guo</surname><given-names>Peng</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/256316/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Qi</surname><given-names>Yi-Ping</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/355327/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Lin-Tong</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/355341/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Ye</surname><given-names>Xin</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/257288/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Jing-Hao</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff4"><sup>4</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/337180/overview"></uri></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Li-Song</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref><xref ref-type="author-notes" rid="fn001"><sup>*</sup></xref><uri xlink:type="simple" xlink:href="http://loop.frontiersin.org/people/229328/overview"></uri></contrib></contrib-group><aff id="aff1"><sup>1</sup><institution>Institute of Plant Nutritional Physiology and Molecular Biology, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></aff><aff id="aff2"><sup>2</sup><institution>Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></aff><aff id="aff3"><sup>3</sup><institution>Institute of Materia Medica, Fujian Academy of Medical Sciences</institution><country>Fuzhou, China</country></aff><aff id="aff4"><sup>4</sup><institution>Pomological Institute, Fujian Academy of Agricultural Sciences</institution><country>Fuzhou, China</country></aff><aff id="aff5"><sup>5</sup><institution>The Higher Educational Key Laboratory of Fujian Province for Soil Ecosystem Health and Regulation, Fujian Agriculture and Forestry University</institution><country>Fuzhou, China</country></aff><author-notes><fn fn-type="edited-by"><p>Edited by: Jianjun Chen, University of Florida, USA</p></fn><fn fn-type="edited-by"><p>Reviewed by: Rupesh Kailasrao Deshmukh, Laval University, Canada; Sylvain Jeandroz, Agrosup Dijon, France</p></fn><corresp id="fn001">*Correspondence: Li-Song Chen <email xlink:type="simple">lisongchen2002@hotmail.com</email></corresp><fn fn-type="other" id="fn002"><p>This article was submitted to Crop Science and Horticulture, a section of the journal Frontiers in Plant Science</p></fn></author-notes><pub-date pub-type="epub"><day>24</day><month>6</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>7</volume><elocation-id>898</elocation-id><history><date date-type="received"><day>26</day><month>3</month><year>2016</year></date><date date-type="accepted"><day>07</day><month>6</month><year>2016</year></date></history><permissions><copyright-statement>Copyright © 2016 Guo, Qi, Yang, Ye, Huang and Chen.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Guo, Qi, Yang, Ye, Huang and Chen</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</license-p></license></permissions><abstract><p>Boron (B) toxicity is observed in some citrus orchards in China. However, limited data are available on the molecular mechanisms of citrus B-toxicity and B-tolerance. Using cDNA-AFLP, we identified 20 up- and 52 down-regulated genes, and 44 up- and 66 down-regulated genes from excess B-treated <italic>Citrus sinensis</italic> and <italic>Citrus grandis</italic> roots, respectively, thereby demonstrating that gene expression profiles were more affected in the latter. In addition, phosphorus and total soluble protein concentrations were lowered only in excess B-treated <italic>C. grandis</italic> roots. Apparently, <italic>C. sinensis</italic> had higher B-tolerance than <italic>C. grandis</italic>. Our results suggested that the following several aspects were responsible for the difference in the B-tolerance between the two citrus species including: (a) B-excess induced <italic>Root Hair Defective 3</italic> expression in <italic>C. sinensis</italic> roots, and repressed <italic>villin4</italic> expression in <italic>C. grandis</italic> roots; accordingly, root growth was less inhibited by B-excess in the former; (b) antioxidant systems were impaired in excess B-treated <italic>C. grandis</italic> roots, hence accelerating root senescence; (c) genes related to Ca<sup>2+</sup> signals were inhibited (induced) by B-excess in <italic>C. grandis</italic> (<italic>C. sinensis</italic>) roots. B-excess-responsive genes related to energy (i.e., <italic>alternative oxidase</italic> and <italic>cytochrome P450</italic>), lipid (i.e., <italic>Glycerol-3-phosphate acyltransferase 9</italic> and <italic>citrus dioxygenase</italic>), and nucleic acid (i.e., <italic>HDA19, histone 4</italic>, and <italic>ribonucleotide reductase RNR1 like protein</italic>) metabolisms also possibly accounted for the difference in the B-tolerance between the two citrus species. These data increased our understanding of the mechanisms on citrus B-toxicity and B-tolerance at transcriptional level.</p></abstract><kwd-group><kwd>boron-excess</kwd><kwd>cDNA-AFLP</kwd><kwd><italic>Citrus grandis</italic></kwd><kwd><italic>Citrus sinensis</italic></kwd><kwd>roots</kwd></kwd-group><funding-group><award-group><funding-source id="cn001">China Agriculture Research System</funding-source><award-id rid="cn001">CARS-27</award-id></award-group></funding-group><counts><fig-count count="5"></fig-count><table-count count="2"></table-count><equation-count count="0"></equation-count><ref-count count="81"></ref-count><page-count count="16"></page-count><word-count count="10419"></word-count></counts></article-meta></front><body><sec sec-type="intro" id="s1"><title>Introduction</title><p>Boron (B), an essential element for normal growth and development of higher plants, is taken up by roots in the form of boric acid from soil solution. Despite this, B is toxic to higher plants when present in excess (Nable et al., <xref rid="B46" ref-type="bibr">1997</xref>; Chen et al., <xref rid="B11" ref-type="bibr">2012</xref>; Sang et al., <xref rid="B58" ref-type="bibr">2015</xref>). Although B-toxicity is less common than B-deficiency, it is still a serious problem in many agricultural regions across the world. However, the molecular mechanisms on B-toxicity and B-tolerance in higher plants are not fully understood.</p><p>In higher plants, B-excess cause impairments of cellular functions and physiological and biochemical processes, such as plant growth (Ayvaz et al., <xref rid="B5" ref-type="bibr">2012</xref>; Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>), hormone balance (Ayvaz et al., <xref rid="B5" ref-type="bibr">2012</xref>, <xref rid="B4" ref-type="bibr">2015</xref>), uptake and use efficiency of other mineral elements (Papadakis et al., <xref rid="B51" ref-type="bibr">2003</xref>; Aquea et al., <xref rid="B2" ref-type="bibr">2012</xref>), leaf photosynthetic electron transport chain (Han et al., <xref rid="B25" ref-type="bibr">2009</xref>), photosynthetic rate (Han et al., <xref rid="B25" ref-type="bibr">2009</xref>; Sheng et al., <xref rid="B60" ref-type="bibr">2010</xref>; Chen et al., <xref rid="B11" ref-type="bibr">2012</xref>; Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>), photosynthetic enzyme activities (Han et al., <xref rid="B25" ref-type="bibr">2009</xref>), reactive oxygen metabolism (Keles et al., <xref rid="B36" ref-type="bibr">2004</xref>; Cervilla et al., <xref rid="B7" ref-type="bibr">2007</xref>; Han et al., <xref rid="B25" ref-type="bibr">2009</xref>), chlorophyll (Chl) and carotenoid (Car) levels (Han et al., <xref rid="B25" ref-type="bibr">2009</xref>; Ayvaz et al., <xref rid="B5" ref-type="bibr">2012</xref>), cell wall biosynthesis (Huang et al., <xref rid="B29" ref-type="bibr">2016</xref>); carbohydrate (Keles et al., <xref rid="B36" ref-type="bibr">2004</xref>; Papadakis et al., <xref rid="B49" ref-type="bibr">2004a</xref>,<xref rid="B50" ref-type="bibr">b</xref>; Han et al., <xref rid="B25" ref-type="bibr">2009</xref>), energy (Reid et al., <xref rid="B56" ref-type="bibr">2004</xref>; Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>), nucleic acid (Wang et al., <xref rid="B74" ref-type="bibr">1995</xref>), and nitrogen (N; Cervilla et al., <xref rid="B8" ref-type="bibr">2009</xref>; Chen et al., <xref rid="B12" ref-type="bibr">2014</xref>) metabolisms, and leaf and root structure (Papadakis et al., <xref rid="B49" ref-type="bibr">2004a</xref>,<xref rid="B50" ref-type="bibr">b</xref>; Chen et al., <xref rid="B11" ref-type="bibr">2012</xref>; Huang et al., <xref rid="B28" ref-type="bibr">2014</xref>; Mesquita et al., <xref rid="B45" ref-type="bibr">2016</xref>).</p><p>Transcriptomic analysis not only offers us the chance to elucidate the molecular mechanisms on plant B-toxicity and B-tolerance but also is very useful for us to obtain B-excess-responsive genes possibly responsible for B-tolerance. There are several studies investigating B-excess-induced alterations of gene profiles in higher plants. Using microarray, Kasajima and Fujiwara (<xref rid="B34" ref-type="bibr">2007</xref>) isolated many high-B-induced genes from <italic>Arabidopsis thaliana</italic> roots and rosette leaves; nine of which were validated in an independent experiment. Using suppression subtractive hybridization (SSH), Hassan et al. (<xref rid="B26" ref-type="bibr">2010</xref>) found 111 up-regulated clones showing similar to known proteins from high-B-treated roots of the B-tolerant barely bulk, thereby suggesting that both polyamines and ascorbate–glutathione pathway played a role in the B-tolerance of barely. Padmanabhan et al. (<xref rid="B47" ref-type="bibr">2012</xref>) used SSH to isolate 219 and 113 unique B-excess-responsive non-redundant expressed sequence tags (ESTs) from extremely B-tolerant <italic>Puccinellia distans</italic> and moderately B-tolerant <italic>Gypsophila arrostil</italic> plants, respectively. In addition to restricting B accumulation in plant tissues <italic>via</italic> enhancing the expression of <italic>efflux transporters</italic> similar to barely <italic>Bot1</italic>, genes related to stress/defense, protein synthesis, cellular organization, and signal transduction had a positive role in the B-tolerance of <italic>P. distans</italic>. Aquea et al. (<xref rid="B2" ref-type="bibr">2012</xref>) obtained 240 up-regulated genes, which mainly function in ABA signaling, ABA response, or cell wall modifications, and 211 down-regulated genes, which are mainly related to glucosinolate biosynthesis, water, or nutrient transport, suggesting that B-toxicity-induced water stress was responsible for root growth inhibition under B-stress. Recently, Tombuloglu et al. (<xref rid="B67" ref-type="bibr">2015</xref>) used high-throughput RNA-Seq to examine B-toxicity-induced alterations of gene profiles in barely and observed a total of 14,385 and 11,472 differentially expressed transcripts from high-B-treated roots and leaves, respectively, and concluded that genes involved in cell wall and cytoskeleton, plasma membrane, stress response, and signal transduction played key roles in the B-tolerance of barely. Although the transcriptional responses of herbaceous plants to B-toxicity have been investigated in some details, such data are very limited in woody plants.</p><p>In China, B-excess is observed in some citrus orchards. Li et al. (<xref rid="B40" ref-type="bibr">2015</xref>) measured the water-soluble B content of 319 soil samples and the B concentration of 319 leaf samples from 319 pummelo (<italic>Citrus grandis</italic>) orchards located in Pinghe, Zhangzhou, China. Up to 22.9 and 74.8% of orchards were excess in soil water-soluble B and leaf B, respectively. Therefore, it is very important to understand the molecular mechanisms on B-toxicity and B-tolerance of citrus. cDNA-amplified fragment length polymorphism (cDNA-AFLP), which does not need prior sequence information for the amplification, is a highly sensitive and reproducible approach for detecting polymorphisms in DNA. Therefore, cDNA-AFLP has been widely used for the identification of various stress-regulated genes and for the isolation of novel genes (Ditt et al., <xref rid="B17" ref-type="bibr">2001</xref>; Fusco et al., <xref rid="B20" ref-type="bibr">2005</xref>; Craciun et al., <xref rid="B15" ref-type="bibr">2006</xref>; Paun and Schönswetter, <xref rid="B52" ref-type="bibr">2012</xref>; Zhou et al., <xref rid="B79" ref-type="bibr">2013</xref>; Vantini et al., <xref rid="B70" ref-type="bibr">2015</xref>). Previously, we used cDNA-AFLP to assay long-term B-excess-responsive genes in B-tolerant <italic>Citrus sinensis</italic> and B-intolerant <italic>C. grandis</italic> leaves and obtained 132 and 68 differentially expressed genes from B-toxic <italic>C. grandis</italic> and <italic>C. sinensis</italic> leaves, respectively. Subsequent analysis suggested that the higher mRNA levels of genes involved in photosynthesis in B-toxic <italic>C. sinensis</italic> leaves were responsible for the higher CO<sub>2</sub> assimilation, thus contributing to the B-tolerance of <italic>C. sinensis</italic>. Some genes related to stress responses also played a role in the B-tolerance of <italic>C. sinensis</italic> (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>). In this study, we further examined long-term B-excess-induced alterations of gene profiles revealed by cDNA-AFLP in B-tolerant <italic>C. sinensis</italic> and B-intolerant <italic>C. grandis</italic> roots (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>; Huang et al., <xref rid="B28" ref-type="bibr">2014</xref>; Sang et al., <xref rid="B58" ref-type="bibr">2015</xref>). The objectives were (a) to determine the differences in B-excess-responsive genes between roots and leaves; (b) to explore the mechanisms on B-toxicity and B-tolerance at translational level; and (c) to obtain B-excess-responsive genes possibly contributing to the B-tolerance of <italic>C. sinensis</italic>.</p></sec><sec sec-type="materials and methods" id="s2"><title>Materials and methods</title><sec><title>Plant materials and experimental design</title><p>Boron-tolerant “Xuegan” (<italic>C. sinensis</italic>) and B-intolerant “Sour pummel” (<italic>C. grandis</italic>) seedlings were used in this study. Both seedling culture and B treatments were performed according to Guo et al. (<xref rid="B24" ref-type="bibr">2014</xref>). In a word, 13-week-old seedlings grown in 6 L pots containing sand in a greenhouse under natural photoperiod at Fujian Agriculture and Forestry University (FAFU), Fuzhou, China (26°5′ N, 119°14′ E), were irrigated every 2 days until dripping with nutrient solution containing 6 mM KNO<sub>3</sub>, 2 mM NH<sub>4</sub>H<sub>2</sub>PO<sub>4</sub>, 4 mM Ca(NO<sub>3</sub>)<sub>2</sub>, 1 mM MgSO<sub>4</sub>, 10 μM H<sub>3</sub>BO<sub>3</sub>, 2 μM ZnSO<sub>4</sub>, 2 μM MnCl<sub>2</sub>, 0.5 μM CuSO<sub>4</sub>, 0.065 μM (NH<sub>4</sub>)<sub>6</sub>Mo<sub>7</sub>O<sub>24</sub> 20 μM Fe-EDTA, and 10 μM (control) or 400 μM (B-excess) H<sub>3</sub>BO<sub>3</sub> for 15 weeks. Thereafter, about 5-mm long root tips from new white roots were excised and immediately immersed in liquid nitrogen, and then stored at −80°C until being used for the measurements of total soluble proteins and enzyme activities, and cDNA-AFLP and qRT-PCR analysis. The remaining seedlings were used to assay root B and phosphorus (P) concentrations.</p></sec><sec><title>Measurements of root total soluble protein, B, and P concentrations</title><p>Root concentration of total soluble proteins was assayed according to Bradford (<xref rid="B6" ref-type="bibr">1976</xref>) after being extracted with 50 mM Na<sub>2</sub>HPO<sub>4</sub>–KH<sub>2</sub>PO<sub>4</sub> (pH 7.0) and 5% (w/v) insoluble polyvinylpyrrolidone. There were five replicates per treatments.</p><p>For the measurements of root B and P concentrations, fibrous roots were harvested and dried at 70°C. Root B concentration was determined by ICP emission spectrometry after microwave digestion with HNO<sub>3</sub> (Wang et al., <xref rid="B73" ref-type="bibr">2006</xref>). Root P concentration was determined according to Ames (<xref rid="B1" ref-type="bibr">1966</xref>). There were four replicates per treatments.</p></sec><sec><title>RNA extraction, cDNA synthesis, and cDNA-AFLP analysis</title><p>Equal amounts of frozen roots from five excess B-treated or control seedlings (one seedling per pot) of <italic>C. grandis</italic> or <italic>C. sinensis</italic> were mixed as a biological replicate. There were three biological replicates for each B treatment. Total RNA was extracted from ca. 300 mg of frozen roots using Recalcitrant Plant Total RNA Extraction Kit (Centrifugal column type, Bioteke Corporation, China). cDNA synthesis and cDNA-AFLP analysis were performed according to Guo et al. (<xref rid="B24" ref-type="bibr">2014</xref>) and Lu et al. (<xref rid="B41" ref-type="bibr">2015</xref>). Database resources of the National Center for Biotechnology Information (NCBI, <ext-link ext-link-type="uri" xlink:href="http://www.ncbi.nlm.nih.gov">http://www.ncbi.nlm.nih.gov</ext-link>) were used for the blast analysis of the differentially expressed transcript-derived fragments (TDFs) from excess B-treated <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots. TDFs were blasted using BLASTX and BLASTN search engines (<ext-link ext-link-type="uri" xlink:href="http://blast.ncbi.nlm.nih.gov/Blast.cgi">http://blast.ncbi.nlm.nih.gov/Blast.cgi</ext-link>), and a TDF was considered to be homologous when it had an <italic>E</italic> < 0.001 and a similarity ≥50%. Their functional categories were assigned based on the Gene Ontology (<ext-link ext-link-type="uri" xlink:href="http://amigo1.geneontology.org/cgi-bin/amigo/go.cgi">http://amigo1.geneontology.org/cgi-bin/amigo/go.cgi</ext-link>) and UniProt (<ext-link ext-link-type="uri" xlink:href="http://www.uniprot.org/">http://www.uniprot.org/</ext-link>) database.</p></sec><sec><title>qRT-PCR analysis</title><p>Root total RNA was extracted as described above. qRT-PCR analysis was carried out according to Zhou et al. (<xref rid="B79" ref-type="bibr">2013</xref>) and Lu et al. (<xref rid="B41" ref-type="bibr">2015</xref>). The sequences of the specific F and R primers designed from the sequences of 15 singleton TDFs using Primer Premier Version 5.0 (PREMIER Biosoft International, CA, USA) were summarized in Table <xref ref-type="supplementary-material" rid="SM1">S1</xref>. The samples subjected to qRT-PCR were performed in three biological replicates with two technical replicates. Each biological replicate was created by pooling equal roots from five seedlings (one seedling per pot). Citrus <italic>actin</italic> (GU911361.1) was used as an internal standard for the normalization of gene expression, and roots from control plants were used as a reference sample (set as 1).</p></sec><sec><title>Analysis of root peroxidase, catalase, and lipoxygenase activities</title><p>Peroxidase (POD) and catalase (CAT) were extracted and assayed according to Li et al. (<xref rid="B39" ref-type="bibr">2010</xref>). Lipoxygenase (LOX) was assayed by the formation of conjugated dienes from linoleic acid according to Axelrod et al. (<xref rid="B3" ref-type="bibr">1981</xref>).</p></sec><sec><title>Statistical analysis</title><p>Differences among four treatment combinations were analyzed by two (B levels) × two (species) ANOVA. Means were separated by the Duncan's new multiple range test at <italic>P</italic> < 0.05. Significant tests for two means (control and B-excess) were carried out by unpaired <italic>t</italic>-test at <italic>P</italic> < 0.05 level.</p></sec></sec><sec sec-type="results" id="s3"><title>Results</title><sec><title>Effects of B-excess on root B, P, and total soluble protein concentrations</title><p>Root B concentration was higher in excess B-treated <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots than in controls. Root B level did not differ significantly between the two citrus species at each given B treatment (Figure <xref ref-type="fig" rid="F1">1A</xref>).</p><fig id="F1" position="float"><label>Figure 1</label><caption><p><bold>Effects of B-excess on root B (A), P (B), and total soluble protein (C) concentrations</bold>. Bars represent means ± <italic>SD</italic> (<italic>n</italic> = 4). Different letters above the bars indicate a significant difference at <italic>P</italic> < 0.05.</p></caption><graphic xlink:href="fpls-07-00898-g0001"></graphic></fig><p>Root P concentration did not significantly differ among four treatment combinations except for a drop in excess B-treated <italic>C. grandis</italic> roots (Figure <xref ref-type="fig" rid="F1">1B</xref>).</p><p>Boron excess only lowered total soluble protein concentration in <italic>C. grandis</italic> roots. Total soluble protein concentration was higher in excess B-treated <italic>C. sinensis</italic> roots than in excess B-treated <italic>C. grandis</italic> but was similar between control roots of the two citrus species (Figure <xref ref-type="fig" rid="F1">1C</xref>).</p></sec><sec><title>Root B-excess-responsive genes revealed by cDNA-AFLP</title><p>A total of 256 selective primer combinations were employed to isolate B-excess-responsive genes from B-tolerant <italic>C. sinensis</italic> and B-intolerant <italic>C. grandis</italic> roots. A representative silver-stained cDNA-AFLP gel displaying the differentially expressed TDFs in excess B-treated <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots was presented in Figure <xref ref-type="fig" rid="F2">2</xref>. As shown in Table <xref ref-type="table" rid="T1">1</xref>, we obtained 5873 TDFs from control and B-toxic <italic>C. sinensis</italic> and <italic>C. grandis</italic> roots, 893 (589) of which were presented only in <italic>C. grandis</italic> (<italic>C. sinensis</italic>) roots and 4391 were shared by the both.</p><fig id="F2" position="float"><label>Figure 2</label><caption><p><bold>cDNA-AFLP profiles using one <italic><bold>Eco</bold></italic>R I selective primer and eight <italic><bold>Mes</bold></italic> I selective primers</bold>. One <italic>Eco</italic>R I selective primer: <italic>Eco</italic>R I-AT; Eight <italic>Mes</italic> I selective primers: <italic>Mes</italic> I-GA, GA, GG, GC, CA, CT, CG, and CC. 1, Control roots of <italic>C. grandis</italic>; 2, Excess B-treated roots of <italic>C. grandis</italic>; 3, Control roots of <italic>C. sinensis</italic>; 4, Excess B-treated roots of <italic>C. sinensis</italic>. Arrows indicate differentially expressed TDFs.</p></caption><graphic xlink:href="fpls-07-00898-g0002"></graphic></fig><table-wrap id="T1" position="float"><label>Table 1</label><caption><p><bold>List of transcript-derived fragments (TDFs) isolated from control and boron (B)-toxic <italic><bold>Citrus grandis</bold></italic> and <italic><bold>Citrus sinensis</bold></italic> roots</bold>.</p></caption><table frame="hsides" rules="groups"><thead><tr><th rowspan="1" colspan="1"></th><th valign="top" align="center" colspan="4" style="border-bottom: thin solid #000000;" rowspan="1"><bold>Number of TDFs</bold></th></tr><tr><th rowspan="1" colspan="1"></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>Only present in <italic>C. grandis</italic></bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>Only present in <italic>C. sinensis</italic></bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>Present in both species</bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>Total</bold></th></tr></thead><tbody><tr><td valign="top" align="left" rowspan="1" colspan="1">Total TDFs detected from gels</td><td valign="top" align="center" rowspan="1" colspan="1">893</td><td valign="top" align="center" rowspan="1" colspan="1">589</td><td valign="top" align="center" rowspan="1" colspan="1">4391</td><td valign="top" align="center" rowspan="1" colspan="1">5873</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">Total differentially expressed TDFs recovered from gels</td><td valign="top" align="center" rowspan="1" colspan="1">156</td><td valign="top" align="center" rowspan="1" colspan="1">82</td><td valign="top" align="center" rowspan="1" colspan="1">56</td><td valign="top" align="center" rowspan="1" colspan="1">294</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">TDFs produced useable sequence data</td><td valign="top" align="center" rowspan="1" colspan="1">133</td><td valign="top" align="center" rowspan="1" colspan="1">65</td><td valign="top" align="center" rowspan="1" colspan="1">43</td><td valign="top" align="center" rowspan="1" colspan="1">241</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">TDFs encoding known or putative proteins</td><td valign="top" align="center" rowspan="1" colspan="1">68</td><td valign="top" align="center" rowspan="1" colspan="1">37</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="center" rowspan="1" colspan="1">123</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">TDFs encoding predicted, uncharacterized, hypothetical, or unnamed proteins</td><td valign="top" align="center" rowspan="1" colspan="1">17</td><td valign="top" align="center" rowspan="1" colspan="1">10</td><td valign="top" align="center" rowspan="1" colspan="1">7</td><td valign="top" align="center" rowspan="1" colspan="1">34</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">TDFs without database matches</td><td valign="top" align="center" rowspan="1" colspan="1">48</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="center" rowspan="1" colspan="1">84</td></tr></tbody></table></table-wrap><p>A 1.5-fold cut-off was employed to determine up- and down-regulated TDFs, in addition to a <italic>P</italic>-value of < 0.05. According to the two criteria, we identified 294 B-excess-responsive TDFs from <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots. After sequencing all these TDFs, we obtained readable sequences from 241 TDFs, 157 of which shared identity with genes encoding known, putative, uncharacterized, predicted, hypothetical, or unnamed proteins, and 84 showed no significant matches (Table <xref ref-type="table" rid="T1">1</xref>). The 157 matched TDFs were related to carbohydrate and energy metabolism, cell wall and cytoskeleton metabolism, lipid metabolism, nucleic acid metabolism, protein and amino acid metabolism, stress response, signal transduction, transport, and others (Table <xref ref-type="table" rid="T2">2</xref> and Figures <xref ref-type="fig" rid="F3">3A,B</xref>). As shown in Tables <xref ref-type="table" rid="T1">1</xref>, <xref ref-type="table" rid="T2">2</xref> and Figure <xref ref-type="fig" rid="F3">3C</xref>, most of these matched B-excess-responsive TDFs only presented in <italic>C. sinensis</italic> (47) or <italic>C. grandis</italic> (85) roots, only 25 TDFs were shared by the both. Obviously, long-term B-excess-induced alterations of gene profiles greatly differed between <italic>C. sinensis</italic> and <italic>C. grandis</italic> roots.</p><table-wrap id="T2" position="float"><label>Table 2</label><caption><p><bold>Homologies of differentially expressed cDNA-AFLP fragments with known gene sequences in database using BLASTN algorithm along their expression patterns in excess B-treated <italic><bold>C. grandis</bold></italic> (CG) and <italic><bold>Citrus sinensis</bold></italic> (CS) roots</bold>.</p></caption><table frame="hsides" rules="groups"><thead><tr><th valign="top" align="left" rowspan="1" colspan="1"><bold>TDF #</bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>Size (bp)</bold></th><th valign="top" align="left" rowspan="1" colspan="1"><bold>Homologies</bold></th><th valign="top" align="left" rowspan="1" colspan="1"><bold>Organism origin</bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold><italic>E</italic>-value</bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>Similarity (%)</bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>Sequence coverage (%)</bold></th><th valign="top" align="left" rowspan="1" colspan="1"><bold>Genebank ID</bold></th><th valign="top" align="center" colspan="2" style="border-bottom: thin solid #000000;" rowspan="1"><bold>Ratio of BE/CK</bold></th></tr><tr><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th rowspan="1" colspan="1"></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>CG</bold></th><th valign="top" align="center" rowspan="1" colspan="1"><bold>CS</bold></th></tr></thead><tbody><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>CARBOHYDRATE AND ENERGY METABOLISM</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">232_1</td><td valign="top" align="center" rowspan="1" colspan="1">286</td><td valign="top" align="left" rowspan="1" colspan="1">Cytochrome c oxidase subunit Vb</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Dimocarpus longan</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-37</td><td valign="top" align="center" rowspan="1" colspan="1">92</td><td valign="top" align="center" rowspan="1" colspan="1">46</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AEQ61828.1">AEQ61828.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">101_5</td><td valign="top" align="center" rowspan="1" colspan="1">179</td><td valign="top" align="left" rowspan="1" colspan="1">NADH dehydrogenase subunit 4, partial (mitochondrion)</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Actinidia polygama</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-26</td><td valign="top" align="center" rowspan="1" colspan="1">96</td><td valign="top" align="center" rowspan="1" colspan="1">9</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CAD60445.1">CAD60445.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">41_2</td><td valign="top" align="center" rowspan="1" colspan="1">315</td><td valign="top" align="left" rowspan="1" colspan="1">Electron transfer flavoprotein-ubiquinone oxidoreductase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-51</td><td valign="top" align="center" rowspan="1" colspan="1">80</td><td valign="top" align="center" rowspan="1" colspan="1">17</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003597528.1">XP_003597528.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.41</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">98_2</td><td valign="top" align="center" rowspan="1" colspan="1">254</td><td valign="top" align="left" rowspan="1" colspan="1">Pyruvate dehydrogenase E1 alpha subunit</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-26</td><td valign="top" align="center" rowspan="1" colspan="1">83</td><td valign="top" align="center" rowspan="1" colspan="1">16</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_171617.1">NP_171617.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.32</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>53_4</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>251</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Pyruvate kinase</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>5.00E-39</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>96</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>12</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_001078275.1">NP_001078275.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0.35</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">185_1</td><td valign="top" align="center" rowspan="1" colspan="1">241</td><td valign="top" align="left" rowspan="1" colspan="1">Phosphoglycerate kinase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-15</td><td valign="top" align="center" rowspan="1" colspan="1">51</td><td valign="top" align="center" rowspan="1" colspan="1">14</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_178073.1">NP_178073.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">150_1</td><td valign="top" align="center" rowspan="1" colspan="1">391</td><td valign="top" align="left" rowspan="1" colspan="1">Alternative oxidase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Mangifera indica</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-33</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">21</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CAA55892.1">CAA55892.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">137_2</td><td valign="top" align="center" rowspan="1" colspan="1">204</td><td valign="top" align="left" rowspan="1" colspan="1">Cytochrome P450</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-19</td><td valign="top" align="center" rowspan="1" colspan="1">66</td><td valign="top" align="center" rowspan="1" colspan="1">11</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAB67854.1">AAB67854.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.29</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">9_2</td><td valign="top" align="center" rowspan="1" colspan="1">201</td><td valign="top" align="left" rowspan="1" colspan="1">Ferredoxin-NADP reductase, root isozyme 2</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">8.00E-30</td><td valign="top" align="center" rowspan="1" colspan="1">86</td><td valign="top" align="center" rowspan="1" colspan="1">15</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_973942.1">NP_973942.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.25</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">26_2</td><td valign="top" align="center" rowspan="1" colspan="1">314</td><td valign="top" align="left" rowspan="1" colspan="1">Dephospho-CoA kinase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-44</td><td valign="top" align="center" rowspan="1" colspan="1">73</td><td valign="top" align="center" rowspan="1" colspan="1">26</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003625901.1">XP_003625901.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.83</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">248_1</td><td valign="top" align="center" rowspan="1" colspan="1">261</td><td valign="top" align="left" rowspan="1" colspan="1">Sucrose synthase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Citrus unshiu</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-44</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="center" rowspan="1" colspan="1">7</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAA89049.1">BAA89049.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.27</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">116_2</td><td valign="top" align="center" rowspan="1" colspan="1">353</td><td valign="top" align="left" rowspan="1" colspan="1">NADP-dependent D-sorbitol-6-phosphate dehydrogenase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-39</td><td valign="top" align="center" rowspan="1" colspan="1">86</td><td valign="top" align="center" rowspan="1" colspan="1">27</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003607080.1">XP_003607080.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.43</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>CELL WALL AND CYTOSKELETON METABOLISM</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">65_1</td><td valign="top" align="left" rowspan="1" colspan="1">305</td><td valign="top" align="left" rowspan="1" colspan="1">Root hair defective 3 GTP-binding protein (RHD3)</td><td valign="top" align="center" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">84</td><td valign="top" align="left" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_177439.2">NP_177439.2</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">151_2</td><td valign="top" align="center" rowspan="1" colspan="1">303</td><td valign="top" align="left" rowspan="1" colspan="1">Alpha-1,4-glucan-protein synthase [UDP-forming], putative</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Ricinus communis</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-60</td><td valign="top" align="center" rowspan="1" colspan="1">92</td><td valign="top" align="center" rowspan="1" colspan="1">30</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002525910.1">XP_002525910.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.34</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">37_2</td><td valign="top" align="center" rowspan="1" colspan="1">240</td><td valign="top" align="left" rowspan="1" colspan="1">Fucosyltransferase 8</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-21</td><td valign="top" align="center" rowspan="1" colspan="1">63</td><td valign="top" align="center" rowspan="1" colspan="1">15</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_001077531.1">NP_001077531.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">99_2</td><td valign="top" align="center" rowspan="1" colspan="1">254</td><td valign="top" align="left" rowspan="1" colspan="1">Polygalacturonase-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-11</td><td valign="top" align="center" rowspan="1" colspan="1">54</td><td valign="top" align="center" rowspan="1" colspan="1">17</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAA95779.1">BAA95779.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">50_1</td><td valign="top" align="center" rowspan="1" colspan="1">255</td><td valign="top" align="left" rowspan="1" colspan="1">Villin 4</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-17</td><td valign="top" align="center" rowspan="1" colspan="1">71</td><td valign="top" align="center" rowspan="1" colspan="1">6</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_194745.1">NP_194745.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">26_1</td><td valign="top" align="center" rowspan="1" colspan="1">385</td><td valign="top" align="left" rowspan="1" colspan="1">O-methyltransferase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Citrus sinensis x Citrus reticulata</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-41</td><td valign="top" align="center" rowspan="1" colspan="1">65</td><td valign="top" align="center" rowspan="1" colspan="1">36</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ABP94018.1">ABP94018.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">231_3</td><td valign="top" align="center" rowspan="1" colspan="1">219</td><td valign="top" align="left" rowspan="1" colspan="1">Beta-D-xylosidase 4</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-29</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">9</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_201262.1">NP_201262.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.46</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">138_1</td><td valign="top" align="center" rowspan="1" colspan="1">415</td><td valign="top" align="left" rowspan="1" colspan="1">Cell wall-associated hydrolase, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-44</td><td valign="top" align="center" rowspan="1" colspan="1">96</td><td valign="top" align="center" rowspan="1" colspan="1">19</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003637074.1">XP_003637074.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">249_1</td><td valign="top" align="center" rowspan="1" colspan="1">384</td><td valign="top" align="left" rowspan="1" colspan="1">Protein SPIRAL1-like2</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-27</td><td valign="top" align="center" rowspan="1" colspan="1">80</td><td valign="top" align="center" rowspan="1" colspan="1">62</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_177083.1">NP_177083.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">3.72</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">253_1</td><td valign="top" align="center" rowspan="1" colspan="1">346</td><td valign="top" align="left" rowspan="1" colspan="1">S-acyltransferase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis lyrata subsp.lyrata</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-39</td><td valign="top" align="center" rowspan="1" colspan="1">76</td><td valign="top" align="center" rowspan="1" colspan="1">15</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002887235.1">XP_002887235.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.51</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>LIPID METABOLISM</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">183_2</td><td valign="top" align="left" rowspan="1" colspan="1">380</td><td valign="top" align="left" rowspan="1" colspan="1">Glycerol-3-phosphate acyltransferase 9</td><td valign="top" align="center" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">9.00E-62</td><td valign="top" align="center" rowspan="1" colspan="1">82</td><td valign="top" align="left" rowspan="1" colspan="1">28</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_568925.1">NP_568925.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.29</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">44_1</td><td valign="top" align="center" rowspan="1" colspan="1">95</td><td valign="top" align="left" rowspan="1" colspan="1">Dihydroxyacetone kinase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-05</td><td valign="top" align="center" rowspan="1" colspan="1">84</td><td valign="top" align="center" rowspan="1" colspan="1">4</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_188404.1">NP_188404.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">249_2</td><td valign="top" align="center" rowspan="1" colspan="1">330</td><td valign="top" align="left" rowspan="1" colspan="1">Coproporphyrinogen III oxidase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-55</td><td valign="top" align="center" rowspan="1" colspan="1">88</td><td valign="top" align="center" rowspan="1" colspan="1">23</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_171847.4">NP_171847.4</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.33</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">24_2</td><td valign="top" align="center" rowspan="1" colspan="1">328</td><td valign="top" align="left" rowspan="1" colspan="1">Cinnamate 4-hydroxylase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Citrus x paradisi</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-27</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="center" rowspan="1" colspan="1">19</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAK57011.1">AAK57011.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.41</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">53_3</td><td valign="top" align="center" rowspan="1" colspan="1">284</td><td valign="top" align="left" rowspan="1" colspan="1">Lipoxygenase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">9.00E-41</td><td valign="top" align="center" rowspan="1" colspan="1">80</td><td valign="top" align="center" rowspan="1" colspan="1">10</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACZ17393.1">ACZ17393.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>129_3</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>290</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Fatty acid/sphingolipid desaturase</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>3.00E-40</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>80</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>17</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_182144.1">NP_182144.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">150_2</td><td valign="top" align="center" rowspan="1" colspan="1">379</td><td valign="top" align="left" rowspan="1" colspan="1">Acyl carrier protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Camellia oleifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-16</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">53</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AFK31314.1">AFK31314.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">89_2</td><td valign="top" align="center" rowspan="1" colspan="1">295</td><td valign="top" align="left" rowspan="1" colspan="1">Acyl carrier protein 3</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Helianthus annuus</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-30</td><td valign="top" align="center" rowspan="1" colspan="1">81</td><td valign="top" align="center" rowspan="1" colspan="1">70</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ADV16367.1">ADV16367.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">9_1</td><td valign="top" align="center" rowspan="1" colspan="1">213</td><td valign="top" align="left" rowspan="1" colspan="1">Citrus dioxygenase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Citrus limetta</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-36</td><td valign="top" align="center" rowspan="1" colspan="1">94</td><td valign="top" align="center" rowspan="1" colspan="1">20</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AER36089.1">AER36089.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">42_1</td><td valign="top" align="center" rowspan="1" colspan="1">261</td><td valign="top" align="left" rowspan="1" colspan="1">1,4-dihydroxy-2-naphthoyl-CoA thioesterase 1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-31</td><td valign="top" align="center" rowspan="1" colspan="1">81</td><td valign="top" align="center" rowspan="1" colspan="1">32</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_175266.1">NP_175266.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">3.61</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">103_6</td><td valign="top" align="center" rowspan="1" colspan="1">152</td><td valign="top" align="left" rowspan="1" colspan="1">JAZ3</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis rupestris</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-11</td><td valign="top" align="center" rowspan="1" colspan="1">70</td><td valign="top" align="center" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AEP60134.1">AEP60134.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.24</td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>NUCLEIC ACID METABOLISM</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">140_2</td><td valign="top" align="left" rowspan="1" colspan="1">249</td><td valign="top" align="left" rowspan="1" colspan="1">Histone deacetylase 19</td><td valign="top" align="center" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-30</td><td valign="top" align="center" rowspan="1" colspan="1">81</td><td valign="top" align="left" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_001078511.1">NP_001078511.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">2.7</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">129_1</td><td valign="top" align="center" rowspan="1" colspan="1">299</td><td valign="top" align="left" rowspan="1" colspan="1">Histone H4</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-32</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="center" rowspan="1" colspan="1">45</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_001077477.1">NP_001077477.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">2.2</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">148_1</td><td valign="top" align="center" rowspan="1" colspan="1">208</td><td valign="top" align="left" rowspan="1" colspan="1">Zinc finger protein CONSTANS-LIKE 5-like</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Glycine max</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_001239972.1">NP_001239972.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">82_1</td><td valign="top" align="center" rowspan="1" colspan="1">213</td><td valign="top" align="left" rowspan="1" colspan="1">DNA binding protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis lyrata subsp. Lyrata</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-04</td><td valign="top" align="center" rowspan="1" colspan="1">74</td><td valign="top" align="center" rowspan="1" colspan="1">5</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002889547.1">XP_002889547.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.5</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">28_1</td><td valign="top" align="center" rowspan="1" colspan="1">308</td><td valign="top" align="left" rowspan="1" colspan="1">PREDICTED: ATP-dependent DNA helicase recG-like</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-15</td><td valign="top" align="center" rowspan="1" colspan="1">53</td><td valign="top" align="center" rowspan="1" colspan="1">10</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002280664.2">XP_002280664.2</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">131_4</td><td valign="top" align="center" rowspan="1" colspan="1">296</td><td valign="top" align="left" rowspan="1" colspan="1">Transcription initiation factor IIB-2</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-51</td><td valign="top" align="center" rowspan="1" colspan="1">93</td><td valign="top" align="center" rowspan="1" colspan="1">23</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_187644.1">NP_187644.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.36</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">60_1</td><td valign="top" align="center" rowspan="1" colspan="1">395</td><td valign="top" align="left" rowspan="1" colspan="1">Poly(A)-binding protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Daucus carota</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-24</td><td valign="top" align="center" rowspan="1" colspan="1">80</td><td valign="top" align="center" rowspan="1" colspan="1">16</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAK30205.1">AAK30205.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>246_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>309</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Transcription factor TCP15</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>1.00E-41</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>89</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>19</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_564973.1">NP_564973.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">79_1</td><td valign="top" align="center" rowspan="1" colspan="1">272</td><td valign="top" align="left" rowspan="1" colspan="1">KNAT4 homeobox protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-47</td><td valign="top" align="center" rowspan="1" colspan="1">99</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CAA63131.1">CAA63131.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">123_2</td><td valign="top" align="center" rowspan="1" colspan="1">287</td><td valign="top" align="left" rowspan="1" colspan="1">DEAD-box ATP-dependent RNA helicase 20</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-30</td><td valign="top" align="center" rowspan="1" colspan="1">77</td><td valign="top" align="center" rowspan="1" colspan="1">16</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_175911.1">NP_175911.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">4.1</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">195_1</td><td valign="top" align="center" rowspan="1" colspan="1">268</td><td valign="top" align="left" rowspan="1" colspan="1">Ribonucleotide reductase RNR1 like protein, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-46</td><td valign="top" align="center" rowspan="1" colspan="1">94</td><td valign="top" align="center" rowspan="1" colspan="1">27</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CAA69026.1">CAA69026.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">133_4</td><td valign="top" align="center" rowspan="1" colspan="1">273</td><td valign="top" align="left" rowspan="1" colspan="1">Putative phosphoribosylaminoimidazole carboxylase/AIR carboxylase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-40</td><td valign="top" align="center" rowspan="1" colspan="1">80</td><td valign="top" align="center" rowspan="1" colspan="1">11</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_181305.2">NP_181305.2</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">134_1</td><td valign="top" align="center" rowspan="1" colspan="1">308</td><td valign="top" align="left" rowspan="1" colspan="1">Polypyrimidine tract-binding protein 1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">9.00E-53</td><td valign="top" align="center" rowspan="1" colspan="1">90</td><td valign="top" align="center" rowspan="1" colspan="1">20</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_186764.1">NP_186764.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">142_1</td><td valign="top" align="center" rowspan="1" colspan="1">231</td><td valign="top" align="left" rowspan="1" colspan="1">Transcription factor/ transcription initiation factor</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-24</td><td valign="top" align="center" rowspan="1" colspan="1">74</td><td valign="top" align="center" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003597404.1">XP_003597404.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.53</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">252_1</td><td valign="top" align="center" rowspan="1" colspan="1">310</td><td valign="top" align="left" rowspan="1" colspan="1">AT1G56110, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-24</td><td valign="top" align="center" rowspan="1" colspan="1">74</td><td valign="top" align="center" rowspan="1" colspan="1">23</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAH20197.1">BAH20197.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.33</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">81_3</td><td valign="top" align="center" rowspan="1" colspan="1">325</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein POPTR_0001s24860g</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Populus trichocarpa</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-49</td><td valign="top" align="center" rowspan="1" colspan="1">79</td><td valign="top" align="center" rowspan="1" colspan="1">14</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002299866.1">XP_002299866.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">1.84</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>PROTEIN AND ACID METABOLISM</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">85_2</td><td valign="top" align="left" rowspan="1" colspan="1">356</td><td valign="top" align="left" rowspan="1" colspan="1">Ubiquitin-protein ligase 1</td><td valign="top" align="center" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-59</td><td valign="top" align="center" rowspan="1" colspan="1">88%</td><td valign="top" align="left" rowspan="1" colspan="1">3%</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAF36454.1">AAF36454.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.38</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>132_3</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>384</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>E3 ubiquitin-protein ligase MARCH3</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Medicago truncatula</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>6.00E-09</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>86</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>27</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003613768.1">XP_003613768.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>73_2</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>324</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>E3 ubiquitin-protein ligase ATL6</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>8.00E-17</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>50</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>23</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAD33584.1">AAD33584.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">42_3</td><td valign="top" align="center" rowspan="1" colspan="1">180</td><td valign="top" align="left" rowspan="1" colspan="1">Ubiquitin, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Oryza sativa</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-26</td><td valign="top" align="center" rowspan="1" colspan="1">96</td><td valign="top" align="center" rowspan="1" colspan="1">46</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAL77200.1">AAL77200.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">86_1</td><td valign="top" align="center" rowspan="1" colspan="1">398</td><td valign="top" align="left" rowspan="1" colspan="1">Ubiquitin-protein ligase, putative</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Ricinus communis</italic></td><td valign="top" align="center" rowspan="1" colspan="1">8.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">57</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002520193.1">XP_002520193.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.43</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">112_1</td><td valign="top" align="center" rowspan="1" colspan="1">461</td><td valign="top" align="left" rowspan="1" colspan="1">E3 ubiquitin-protein ligase UPL5</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-42</td><td valign="top" align="center" rowspan="1" colspan="1">70</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003594229.1">XP_003594229.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">27_1</td><td valign="top" align="center" rowspan="1" colspan="1">333</td><td valign="top" align="left" rowspan="1" colspan="1">ARM repeat superfamily protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-52</td><td valign="top" align="center" rowspan="1" colspan="1">83</td><td valign="top" align="center" rowspan="1" colspan="1">6</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_198149.2">NP_198149.2</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">230_1</td><td valign="top" align="center" rowspan="1" colspan="1">258</td><td valign="top" align="left" rowspan="1" colspan="1">Aspartic protease, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Dimocarpus longan</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-05</td><td valign="top" align="center" rowspan="1" colspan="1">71</td><td valign="top" align="center" rowspan="1" colspan="1">26</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AEJ76922.1">AEJ76922.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>62_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>326</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Aspartic proteinase nepenthesin-1</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Medicago truncatula</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>1.00E-37</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>77</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>19</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003627883.1">XP_003627883.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">61_1</td><td valign="top" align="center" rowspan="1" colspan="1">335</td><td valign="top" align="left" rowspan="1" colspan="1">Protein ASPARTIC PROTEASE IN GUARD CELL 1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-40</td><td valign="top" align="center" rowspan="1" colspan="1">65</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_188478.1">NP_188478.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">1.79</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">118_5</td><td valign="top" align="center" rowspan="1" colspan="1">208</td><td valign="top" align="left" rowspan="1" colspan="1">Serine carboxypeptidase II-3</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-22</td><td valign="top" align="center" rowspan="1" colspan="1">72</td><td valign="top" align="center" rowspan="1" colspan="1">14</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003592243.1">XP_003592243.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.21</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">41_1</td><td valign="top" align="center" rowspan="1" colspan="1">141</td><td valign="top" align="left" rowspan="1" colspan="1">Drought-inducible cysteine proteinase RD19A precursor</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-16</td><td valign="top" align="center" rowspan="1" colspan="1">86</td><td valign="top" align="center" rowspan="1" colspan="1">35</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAD94010.1">BAD94010.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">24_1</td><td valign="top" align="center" rowspan="1" colspan="1">352</td><td valign="top" align="left" rowspan="1" colspan="1">Gamma-glutamyl hydrolase 2</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-34</td><td valign="top" align="center" rowspan="1" colspan="1">74</td><td valign="top" align="center" rowspan="1" colspan="1">25</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_565186.2">NP_565186.2</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.35</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">253_4</td><td valign="top" align="center" rowspan="1" colspan="1">264</td><td valign="top" align="left" rowspan="1" colspan="1">Protein disulfide isomerase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Zea mays</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-23</td><td valign="top" align="center" rowspan="1" colspan="1">64</td><td valign="top" align="center" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACG47473.1">ACG47473.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.43</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">184_1</td><td valign="top" align="center" rowspan="1" colspan="1">267</td><td valign="top" align="left" rowspan="1" colspan="1">Protein disulfide isomerase-like 1-1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-09</td><td valign="top" align="center" rowspan="1" colspan="1">56</td><td valign="top" align="center" rowspan="1" colspan="1">17</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_849696.1">NP_849696.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.38</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">12_1</td><td valign="top" align="center" rowspan="1" colspan="1">361</td><td valign="top" align="left" rowspan="1" colspan="1">Elongation factor 1-alpha, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-21</td><td valign="top" align="center" rowspan="1" colspan="1">93</td><td valign="top" align="center" rowspan="1" colspan="1">60</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAD94755.1">BAD94755.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">2.04</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">121_2</td><td valign="top" align="center" rowspan="1" colspan="1">160</td><td valign="top" align="left" rowspan="1" colspan="1">40S ribosomal protein S8</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Zea mays</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-19</td><td valign="top" align="center" rowspan="1" colspan="1">98</td><td valign="top" align="center" rowspan="1" colspan="1">19</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_001105391.1">NP_001105391.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">2.52</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">48_1</td><td valign="top" align="center" rowspan="1" colspan="1">303</td><td valign="top" align="left" rowspan="1" colspan="1">40S ribosomal protein S4</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-52</td><td valign="top" align="center" rowspan="1" colspan="1">90</td><td valign="top" align="center" rowspan="1" colspan="1">24</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003604568.1">XP_003604568.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.44</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">246_5</td><td valign="top" align="center" rowspan="1" colspan="1">228</td><td valign="top" align="left" rowspan="1" colspan="1">40S ribosomal protein S2</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis lyrata subsp. Lyrata</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-29</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">27</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002878157.1">XP_002878157.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>9_3</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>198</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>60S ribosomal protein L7a</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Zea mays</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>2.00E-15</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>91</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>19</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACG47588.1">ACG47588.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>153_2</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>261</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>40S ribosomal protein S5</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Capsicum annuum</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>4.00E-44</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>100</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>31</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAR89617.1">AAR89617.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0.39</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>80_2</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>320</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>50S ribosomal protein L2, chloroplastic</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Citrus sinensis</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>3.00E-63</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>99</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>62</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="YP_740517.1">YP_740517.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">64_2</td><td valign="top" align="center" rowspan="1" colspan="1">291</td><td valign="top" align="left" rowspan="1" colspan="1">60S ribosomal protein L10-1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-54</td><td valign="top" align="center" rowspan="1" colspan="1">90</td><td valign="top" align="center" rowspan="1" colspan="1">30</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_563945.2">NP_563945.2</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">140_1</td><td valign="top" align="center" rowspan="1" colspan="1">335</td><td valign="top" align="left" rowspan="1" colspan="1">40S ribosomal protein S29</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Zea mays</italic></td><td valign="top" align="center" rowspan="1" colspan="1">9.00E-26</td><td valign="top" align="center" rowspan="1" colspan="1">86</td><td valign="top" align="center" rowspan="1" colspan="1">74</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACG30830.1">ACG30830.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">3.12</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">38_1</td><td valign="top" align="center" rowspan="1" colspan="1">290</td><td valign="top" align="left" rowspan="1" colspan="1">60S ribosomal protein L15-1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-47</td><td valign="top" align="center" rowspan="1" colspan="1">84</td><td valign="top" align="center" rowspan="1" colspan="1">33</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_193405.1">NP_193405.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.41</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">14_2</td><td valign="top" align="center" rowspan="1" colspan="1">225</td><td valign="top" align="left" rowspan="1" colspan="1">Translation initiation factor eIF-4A1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-37</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="center" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CAC43286.1">CAC43286.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>229_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>327</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Methionine synthase 2</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>3.00E-59</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>92</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>12</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_187028.1">NP_187028.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0.46</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>144_2</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>266</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Threonyl-tRNA synthetase</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Medicago truncatula</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>2.00E-41</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>88</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>11</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003601575.1">XP_003601575.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0.22</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>5_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>354</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>S-adenosyl-L-homocystein hydrolase</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Gossypium hirsutum</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>2.00E-27</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>89</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>19</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACJ11250.1">ACJ11250.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">229_2</td><td valign="top" align="center" rowspan="1" colspan="1">192</td><td valign="top" align="left" rowspan="1" colspan="1">Bifunctional aminoacyl-tRNA synthetase, putative, expressed</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Oryza sativa Japonica Group</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-30</td><td valign="top" align="center" rowspan="1" colspan="1">96</td><td valign="top" align="center" rowspan="1" colspan="1">10</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ABA97740.1">ABA97740.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.34</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>STRESS RESPONSE</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1"><bold>182_3</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>214</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Homogentisate phytyltransferase 1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>3.00E-14</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>51</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>12</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_849984.1">NP_849984.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">64_1</td><td valign="top" align="center" rowspan="1" colspan="1">309</td><td valign="top" align="left" rowspan="1" colspan="1">Obg-like ATPase 1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-51</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">20</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_174346.1">NP_174346.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">99_3</td><td valign="top" align="center" rowspan="1" colspan="1">238</td><td valign="top" align="left" rowspan="1" colspan="1">Peroxidase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-22</td><td valign="top" align="center" rowspan="1" colspan="1">60</td><td valign="top" align="center" rowspan="1" colspan="1">14</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003615990.1">XP_003615990.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>231_5</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>188</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Catalase, partial</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Citrus maxima</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>9.00E-31</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>91</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>19</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACY30463.1">ACY30463.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">30_1</td><td valign="top" align="center" rowspan="1" colspan="1">320</td><td valign="top" align="left" rowspan="1" colspan="1">DJ-1 family protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis lyrata subsp.lyrata</italic></td><td valign="top" align="center" rowspan="1" colspan="1">9.00E-28</td><td valign="top" align="center" rowspan="1" colspan="1">73</td><td valign="top" align="center" rowspan="1" colspan="1">24</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002894433.1">XP_002894433.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.24</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">233_5</td><td valign="top" align="center" rowspan="1" colspan="1">141</td><td valign="top" align="left" rowspan="1" colspan="1">Heat shock protein 70</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Nicotiana benthamiana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-05</td><td valign="top" align="center" rowspan="1" colspan="1">75</td><td valign="top" align="center" rowspan="1" colspan="1">33</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAD02271.1">BAD02271.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">237_1</td><td valign="top" align="center" rowspan="1" colspan="1">305</td><td valign="top" align="left" rowspan="1" colspan="1">BAG family molecular chaperone regulator 7</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-14</td><td valign="top" align="center" rowspan="1" colspan="1">67</td><td valign="top" align="center" rowspan="1" colspan="1">16</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_201045.1">NP_201045.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.35</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">131_1</td><td valign="top" align="center" rowspan="1" colspan="1">332</td><td valign="top" align="left" rowspan="1" colspan="1">Putative senescence-associated protein, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Trichosanthes dioica</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-05</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ABN50029.1">ABN50029.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">17_1</td><td valign="top" align="center" rowspan="1" colspan="1">296</td><td valign="top" align="left" rowspan="1" colspan="1">Putative senescence-associated protein, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Ipomoea ni</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-52</td><td valign="top" align="center" rowspan="1" colspan="1">93</td><td valign="top" align="center" rowspan="1" colspan="1">25</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAF46313.1">BAF46313.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.52</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">104_3</td><td valign="top" align="center" rowspan="1" colspan="1">171</td><td valign="top" align="left" rowspan="1" colspan="1">WD repeat phosphoinositide-interacting-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-21</td><td valign="top" align="center" rowspan="1" colspan="1">96</td><td valign="top" align="center" rowspan="1" colspan="1">11</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003591137.1">XP_003591137.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">3.15</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">77_5</td><td valign="top" align="center" rowspan="1" colspan="1">112</td><td valign="top" align="left" rowspan="1" colspan="1">Universal stress protein A-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-09</td><td valign="top" align="center" rowspan="1" colspan="1">74</td><td valign="top" align="center" rowspan="1" colspan="1">12</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003616191.1">XP_003616191.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.37</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">140_3</td><td valign="top" align="center" rowspan="1" colspan="1">220</td><td valign="top" align="left" rowspan="1" colspan="1">ACR toxin-sensitivity inducing protein (mitochondrion)</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Citrus jambhiri</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-10</td><td valign="top" align="center" rowspan="1" colspan="1">96</td><td valign="top" align="center" rowspan="1" colspan="1">10</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAB85481.1">BAB85481.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">251_5</td><td valign="top" align="center" rowspan="1" colspan="1">266</td><td valign="top" align="left" rowspan="1" colspan="1">Ankyrin-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-38</td><td valign="top" align="center" rowspan="1" colspan="1">82</td><td valign="top" align="center" rowspan="1" colspan="1">9</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAB10271.1">BAB10271.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">146_5</td><td valign="top" align="center" rowspan="1" colspan="1">285</td><td valign="top" align="left" rowspan="1" colspan="1">Leucine-rich repeat containing protein, putative</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Ricinus communis</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-20</td><td valign="top" align="center" rowspan="1" colspan="1">59</td><td valign="top" align="center" rowspan="1" colspan="1">8</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002523984.1">XP_002523984.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>SIGNAL TRANSDUCTION</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">35_1</td><td valign="top" align="left" rowspan="1" colspan="1">341</td><td valign="top" align="left" rowspan="1" colspan="1">ATP/GTP/Ca++ binding protein</td><td valign="top" align="center" rowspan="1" colspan="1"><italic>Cucumis melo subsp.melo</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-44</td><td valign="top" align="center" rowspan="1" colspan="1">74</td><td valign="top" align="left" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ABR67417.1">ABR67417.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">3.04</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">80_3</td><td valign="top" align="center" rowspan="1" colspan="1">194</td><td valign="top" align="left" rowspan="1" colspan="1">Calcium-dependent lipid-binding domain-containing protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-19</td><td valign="top" align="center" rowspan="1" colspan="1">80</td><td valign="top" align="center" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_564576.1">NP_564576.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.41</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">67_2</td><td valign="top" align="center" rowspan="1" colspan="1">232</td><td valign="top" align="left" rowspan="1" colspan="1">Calcium-binding EF-hand-containing protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-07</td><td valign="top" align="center" rowspan="1" colspan="1">52</td><td valign="top" align="center" rowspan="1" colspan="1">5</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_173582.2">NP_173582.2</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.49</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">35_2</td><td valign="top" align="center" rowspan="1" colspan="1">267</td><td valign="top" align="left" rowspan="1" colspan="1">Calcium ion binding protein, putative</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Ricinus communis</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-32</td><td valign="top" align="center" rowspan="1" colspan="1">67</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002534034.1">XP_002534034.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.48</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">103_2</td><td valign="top" align="center" rowspan="1" colspan="1">207</td><td valign="top" align="left" rowspan="1" colspan="1">Serine/threonine-protein kinase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Nicotiana attenuata</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-20</td><td valign="top" align="center" rowspan="1" colspan="1">71</td><td valign="top" align="center" rowspan="1" colspan="1">8</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AEI84329.1">AEI84329.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>251_3</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>301</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Leucine-rich repeat receptor-like protein kinase</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Medicago truncatula</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>4.00E-34</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>68</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>15</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003600547.1">XP_003600547.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">53_2</td><td valign="top" align="center" rowspan="1" colspan="1">350</td><td valign="top" align="left" rowspan="1" colspan="1">ATP binding/kinase/protein serine/threonine kinase</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-50</td><td valign="top" align="center" rowspan="1" colspan="1">80</td><td valign="top" align="center" rowspan="1" colspan="1">12</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CAQ58615.1">CAQ58615.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">122_1</td><td valign="top" align="center" rowspan="1" colspan="1">343</td><td valign="top" align="left" rowspan="1" colspan="1">Receptor-like protein kinase-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Glycine max</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-46</td><td valign="top" align="center" rowspan="1" colspan="1">77</td><td valign="top" align="center" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACM89521.1">ACM89521.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">103_3</td><td valign="top" align="center" rowspan="1" colspan="1">207</td><td valign="top" align="left" rowspan="1" colspan="1">14-3-3 protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Litchi chinensis</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-33</td><td valign="top" align="center" rowspan="1" colspan="1">95</td><td valign="top" align="center" rowspan="1" colspan="1">19</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ADP00759.1">ADP00759.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">117_1</td><td valign="top" align="center" rowspan="1" colspan="1">184</td><td valign="top" align="left" rowspan="1" colspan="1">Auxin-responsive protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Gossypium hirsutum</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-07</td><td valign="top" align="center" rowspan="1" colspan="1">54</td><td valign="top" align="center" rowspan="1" colspan="1">15</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AEE25651.1">AEE25651.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">39_1</td><td valign="top" align="center" rowspan="1" colspan="1">330</td><td valign="top" align="left" rowspan="1" colspan="1">Phytochrome A, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Populus tremula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-51</td><td valign="top" align="center" rowspan="1" colspan="1">86</td><td valign="top" align="center" rowspan="1" colspan="1">7</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AEK26583.1">AEK26583.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>TRANSPORT</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1"><bold>251_2</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>316</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>H</bold>+<bold>-ATPase 4, plasma membrane-type</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>3.00E-23</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>92</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>10</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_190378.2">NP_190378.2</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>76_3</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>270</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Exocyst complex component 84B</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>8.00E-25</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>64</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>9</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_199794.1">NP_199794.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">77_8</td><td valign="top" align="center" rowspan="1" colspan="1">448</td><td valign="top" align="left" rowspan="1" colspan="1">Vacuolar membrane ATPase subunit c”</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Citrus limon</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-71</td><td valign="top" align="center" rowspan="1" colspan="1">99</td><td valign="top" align="center" rowspan="1" colspan="1">50</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAO73433.1">AAO73433.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">151_1</td><td valign="top" align="center" rowspan="1" colspan="1">336</td><td valign="top" align="left" rowspan="1" colspan="1">Exocyst subunit exo70 family protein B1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-50</td><td valign="top" align="center" rowspan="1" colspan="1">82</td><td valign="top" align="center" rowspan="1" colspan="1">15</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_200651.1">NP_200651.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">135_1</td><td valign="top" align="center" rowspan="1" colspan="1">326</td><td valign="top" align="left" rowspan="1" colspan="1">ADP ribosylation factor</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-50</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="center" rowspan="1" colspan="1">88</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003591354.1">XP_003591354.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.49</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">246_4</td><td valign="top" align="center" rowspan="1" colspan="1">228</td><td valign="top" align="left" rowspan="1" colspan="1">Peroxin 7</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-07</td><td valign="top" align="center" rowspan="1" colspan="1">75</td><td valign="top" align="center" rowspan="1" colspan="1">16</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_174220.1">NP_174220.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.45</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">39_2</td><td valign="top" align="center" rowspan="1" colspan="1">216</td><td valign="top" align="left" rowspan="1" colspan="1">Protein transport protein SEC61 gamma subunit</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-04</td><td valign="top" align="center" rowspan="1" colspan="1">90</td><td valign="top" align="center" rowspan="1" colspan="1">64</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003616767.1">XP_003616767.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">183_3</td><td valign="top" align="center" rowspan="1" colspan="1">236</td><td valign="top" align="left" rowspan="1" colspan="1">ABC transporter I family member 20</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-12</td><td valign="top" align="center" rowspan="1" colspan="1">69</td><td valign="top" align="center" rowspan="1" colspan="1">20</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_195847.1">NP_195847.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.42</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">253_2</td><td valign="top" align="center" rowspan="1" colspan="1">318</td><td valign="top" align="left" rowspan="1" colspan="1">Metal tolerance protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Carica papaya</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-52</td><td valign="top" align="center" rowspan="1" colspan="1">89</td><td valign="top" align="center" rowspan="1" colspan="1">26</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ADI24923.1">ADI24923.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">19_1</td><td valign="top" align="center" rowspan="1" colspan="1">316</td><td valign="top" align="left" rowspan="1" colspan="1">Sec61 transport protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Populus trichocarpa</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-54</td><td valign="top" align="center" rowspan="1" colspan="1">88</td><td valign="top" align="center" rowspan="1" colspan="1">16</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002331716.1">XP_002331716.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.5</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">141_2</td><td valign="top" align="center" rowspan="1" colspan="1">272</td><td valign="top" align="left" rowspan="1" colspan="1">Synaptobrevin-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-35</td><td valign="top" align="center" rowspan="1" colspan="1">83</td><td valign="top" align="center" rowspan="1" colspan="1">20</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003617238.1">XP_003617238.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">79_3</td><td valign="top" align="center" rowspan="1" colspan="1">196</td><td valign="top" align="left" rowspan="1" colspan="1">PRA1 (prenylated RAB acceptor) family protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-18</td><td valign="top" align="center" rowspan="1" colspan="1">65</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003597623.1">XP_003597623.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.31</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">10_1</td><td valign="top" align="center" rowspan="1" colspan="1">147</td><td valign="top" align="left" rowspan="1" colspan="1">Protein tolB</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-09</td><td valign="top" align="center" rowspan="1" colspan="1">72</td><td valign="top" align="center" rowspan="1" colspan="1">7</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003637684.1">XP_003637684.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.37</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" colspan="10" style="background-color:#bbbdc0" rowspan="1"><bold>OTHERS</bold></td></tr><tr><td valign="top" align="center" rowspan="1" colspan="1">98_3</td><td valign="top" align="left" rowspan="1" colspan="1">331</td><td valign="top" align="left" rowspan="1" colspan="1">Rubber elongation factor protein</td><td valign="top" align="center" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-23</td><td valign="top" align="center" rowspan="1" colspan="1">63</td><td valign="top" align="left" rowspan="1" colspan="1">31</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_187201.1">NP_187201.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">133_5</td><td valign="top" align="center" rowspan="1" colspan="1">259</td><td valign="top" align="left" rowspan="1" colspan="1">FRIGIDA-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">9.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">69</td><td valign="top" align="center" rowspan="1" colspan="1">12</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_850923.1">NP_850923.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>121_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>248</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Limonoid UDP-glucosyltransferase</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Citrus maxima</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>5.00E-11</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>70</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>16</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ABY27084.1">ABY27084.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">77_4</td><td valign="top" align="center" rowspan="1" colspan="1">318</td><td valign="top" align="left" rowspan="1" colspan="1">ATP sulfurylase 1</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-54</td><td valign="top" align="center" rowspan="1" colspan="1">97</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_188929.1">NP_188929.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">38_2</td><td valign="top" align="center" rowspan="1" colspan="1">134</td><td valign="top" align="left" rowspan="1" colspan="1">FAD-binding berberine family protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-13</td><td valign="top" align="center" rowspan="1" colspan="1">85</td><td valign="top" align="center" rowspan="1" colspan="1">6</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003594602.1">XP_003594602.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.41</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">189_1</td><td valign="top" align="center" rowspan="1" colspan="1">309</td><td valign="top" align="left" rowspan="1" colspan="1">Probable methyltransferase PMT14</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-37</td><td valign="top" align="center" rowspan="1" colspan="1">62</td><td valign="top" align="center" rowspan="1" colspan="1">15</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAH19630.1">BAH19630.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">41_3</td><td valign="top" align="center" rowspan="1" colspan="1">398</td><td valign="top" align="left" rowspan="1" colspan="1">1-deoxy-D-xylulose-5-phosphate synthase, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Bixa orellana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-65</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">68</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAU29063.1">AAU29063.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">1.9</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">173_1</td><td valign="top" align="center" rowspan="1" colspan="1">286</td><td valign="top" align="left" rowspan="1" colspan="1">DPP6 N-terminal domain-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-21</td><td valign="top" align="center" rowspan="1" colspan="1">70</td><td valign="top" align="center" rowspan="1" colspan="1">12</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="NP_564147.1">NP_564147.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.3</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">66_1</td><td valign="top" align="center" rowspan="1" colspan="1">284</td><td valign="top" align="left" rowspan="1" colspan="1">F22G5.28</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis thaliana</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-36</td><td valign="top" align="center" rowspan="1" colspan="1">68</td><td valign="top" align="center" rowspan="1" colspan="1">12</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="AAF79556.1">AAF79556.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">147_4</td><td valign="top" align="center" rowspan="1" colspan="1">254</td><td valign="top" align="left" rowspan="1" colspan="1">PREDICTED: uncharacterized protein LOC100264520</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-38</td><td valign="top" align="center" rowspan="1" colspan="1">84</td><td valign="top" align="center" rowspan="1" colspan="1">24</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002276822.1">XP_002276822.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.51</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">109_3</td><td valign="top" align="center" rowspan="1" colspan="1">277</td><td valign="top" align="left" rowspan="1" colspan="1">Unnamed protein product, partial</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-19</td><td valign="top" align="center" rowspan="1" colspan="1">64</td><td valign="top" align="center" rowspan="1" colspan="1">50</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CBI31118.3">CBI31118.3</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.28</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">153_3</td><td valign="top" align="center" rowspan="1" colspan="1">226</td><td valign="top" align="left" rowspan="1" colspan="1">Predicted protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis lyrata subsp</italic>.</td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-16</td><td valign="top" align="center" rowspan="1" colspan="1">69</td><td valign="top" align="center" rowspan="1" colspan="1">46</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002871569.1">XP_002871569.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">1.78</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">22_4</td><td valign="top" align="center" rowspan="1" colspan="1">287</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein VITISV_014691</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-27</td><td valign="top" align="center" rowspan="1" colspan="1">65</td><td valign="top" align="center" rowspan="1" colspan="1">21</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CAN71367.1">CAN71367.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.32</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">158_1</td><td valign="top" align="center" rowspan="1" colspan="1">177</td><td valign="top" align="left" rowspan="1" colspan="1">PREDICTED: uncharacterized protein LOC100255693</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-09</td><td valign="top" align="center" rowspan="1" colspan="1">51</td><td valign="top" align="center" rowspan="1" colspan="1">13</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002279857.1">XP_002279857.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">1.33</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">21_1</td><td valign="top" align="center" rowspan="1" colspan="1">231</td><td valign="top" align="left" rowspan="1" colspan="1">AT5G10200-like protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Arabidopsis arenosa</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-31</td><td valign="top" align="center" rowspan="1" colspan="1">90</td><td valign="top" align="center" rowspan="1" colspan="1">9</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACK44505.1">ACK44505.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">163_3</td><td valign="top" align="center" rowspan="1" colspan="1">228</td><td valign="top" align="left" rowspan="1" colspan="1">PREDICTED: uncharacterized protein LOC100262433</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Vitis vinifera</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-30</td><td valign="top" align="center" rowspan="1" colspan="1">77</td><td valign="top" align="center" rowspan="1" colspan="1">11</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002284886.1">XP_002284886.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">27_2</td><td valign="top" align="center" rowspan="1" colspan="1">267</td><td valign="top" align="left" rowspan="1" colspan="1">Predicted protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Populus trichocarpa</italic></td><td valign="top" align="center" rowspan="1" colspan="1">5.00E-27</td><td valign="top" align="center" rowspan="1" colspan="1">63</td><td valign="top" align="center" rowspan="1" colspan="1">30</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002330640.1">XP_002330640.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">116_1</td><td valign="top" align="center" rowspan="1" colspan="1">401</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein MTR_1g005920</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">79</td><td valign="top" align="center" rowspan="1" colspan="1">75</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003588318.1">XP_003588318.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.39</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>163_2</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>330</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Unnamed protein product</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Vitis vinifera</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>8.00E-07</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>69</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>6</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CBI37596.3">CBI37596.3</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">170_2</td><td valign="top" align="center" rowspan="1" colspan="1">212</td><td valign="top" align="left" rowspan="1" colspan="1">Predicted protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Populus trichocarpa</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-07</td><td valign="top" align="center" rowspan="1" colspan="1">68</td><td valign="top" align="center" rowspan="1" colspan="1">14</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002309501.1">XP_002309501.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">77_6</td><td valign="top" align="center" rowspan="1" colspan="1">230</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein MTR_5g051130</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-11</td><td valign="top" align="center" rowspan="1" colspan="1">79</td><td valign="top" align="center" rowspan="1" colspan="1">6</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003614394.1">XP_003614394.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.51</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">105_2</td><td valign="top" align="center" rowspan="1" colspan="1">277</td><td valign="top" align="left" rowspan="1" colspan="1">PREDICTED: UPF0197 transmembrane protein C11orf10</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Glycine max</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">87</td><td valign="top" align="center" rowspan="1" colspan="1">42</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003549239.1">XP_003549239.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.13</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>108_2</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>144</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Hypothetical protein SORBIDRAFT_0351s002020</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Sorghum bicolor</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>7.00E-16</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>100</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>28</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002489033.1">XP_002489033.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">109_5</td><td valign="top" align="center" rowspan="1" colspan="1">178</td><td valign="top" align="left" rowspan="1" colspan="1">Unknown</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Glycine max</italic></td><td valign="top" align="center" rowspan="1" colspan="1">6.00E-15</td><td valign="top" align="center" rowspan="1" colspan="1">89</td><td valign="top" align="center" rowspan="1" colspan="1">57</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACU14517.1">ACU14517.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">118_3</td><td valign="top" align="center" rowspan="1" colspan="1">287</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein MTR_5g051140</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-14</td><td valign="top" align="center" rowspan="1" colspan="1">93</td><td valign="top" align="center" rowspan="1" colspan="1">8</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003614395.1">XP_003614395.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">2.47</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>170_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>296</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Unknown</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Glycine max</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>5.00E-31</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>95</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>30</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACU24256.1">ACU24256.1</ext-link></bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>1.8</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>2.11</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">173_3</td><td valign="top" align="center" rowspan="1" colspan="1">213</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein MTR_5g051120</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">1.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">88</td><td valign="top" align="center" rowspan="1" colspan="1">18</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003614393.1">XP_003614393.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">1.73</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>178_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>306</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Hypothetical protein</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Lilium longiflorum</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>2.00E-25</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>96</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>100</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ABO20854.1">ABO20854.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">193_3</td><td valign="top" align="center" rowspan="1" colspan="1">244</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein MTR_4g091430</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-11</td><td valign="top" align="center" rowspan="1" colspan="1">57</td><td valign="top" align="center" rowspan="1" colspan="1">48</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003608262.1">XP_003608262.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>228_1</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>306</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Hypothetical protein</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Arabidopsis thaliana</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>1.00E-39</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>100</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>11</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="BAF01964.1">BAF01964.1</ext-link></bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>1.32</bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">237_3</td><td valign="top" align="center" rowspan="1" colspan="1">204</td><td valign="top" align="left" rowspan="1" colspan="1">PREDICTED: uncharacterized protein LOC100800109</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Glycine max</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-31</td><td valign="top" align="center" rowspan="1" colspan="1">92</td><td valign="top" align="center" rowspan="1" colspan="1">54</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003541224.1">XP_003541224.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">+</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">249_5</td><td valign="top" align="center" rowspan="1" colspan="1">224</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Zea mays</italic></td><td valign="top" align="center" rowspan="1" colspan="1">4.00E-20</td><td valign="top" align="center" rowspan="1" colspan="1">98</td><td valign="top" align="center" rowspan="1" colspan="1">24</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="ACG27665.1">ACG27665.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">1.38</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>37_3</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>217</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Hypothetical protein SORBIDRAFT_0070s002020</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Sorghum bicolor</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>3.00E-23</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>82</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>72</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002489102.1">XP_002489102.1</ext-link></bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>3.01</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0.39</bold></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">53_5</td><td valign="top" align="center" rowspan="1" colspan="1">165</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein SORBIDRAFT_1994s002010</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Sorghum bicolor</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-10</td><td valign="top" align="center" rowspan="1" colspan="1">100</td><td valign="top" align="center" rowspan="1" colspan="1">28</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002488913.1">XP_002488913.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.73</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">6_3</td><td valign="top" align="center" rowspan="1" colspan="1">218</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein MTR_5g051030</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">8.00E-25</td><td valign="top" align="center" rowspan="1" colspan="1">95</td><td valign="top" align="center" rowspan="1" colspan="1">9</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003614386.1">XP_003614386.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">2.06</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">85_1</td><td valign="top" align="center" rowspan="1" colspan="1">369</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein SORBIDRAFT_1368s002010</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Sorghum bicolor</italic></td><td valign="top" align="center" rowspan="1" colspan="1">7.00E-52</td><td valign="top" align="center" rowspan="1" colspan="1">91</td><td valign="top" align="center" rowspan="1" colspan="1">66</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002488947.1">XP_002488947.1</ext-link></td><td rowspan="1" colspan="1"></td><td valign="top" align="center" rowspan="1" colspan="1">0.47</td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">94_1</td><td valign="top" align="center" rowspan="1" colspan="1">222</td><td valign="top" align="left" rowspan="1" colspan="1">Hypothetical protein MTR_8g040260</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Medicago truncatula</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-06</td><td valign="top" align="center" rowspan="1" colspan="1">51</td><td valign="top" align="center" rowspan="1" colspan="1">31</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_003627937.1">XP_003627937.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">146_4</td><td valign="top" align="center" rowspan="1" colspan="1">245</td><td valign="top" align="left" rowspan="1" colspan="1">Putative uncharacterized protein Sb0351s002020</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Sorghum bicolor</italic></td><td valign="top" align="center" rowspan="1" colspan="1">3.00E-12</td><td valign="top" align="center" rowspan="1" colspan="1">97</td><td valign="top" align="center" rowspan="1" colspan="1">47</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002489033.1">XP_002489033.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.54</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1">252_4</td><td valign="top" align="center" rowspan="1" colspan="1">190</td><td valign="top" align="left" rowspan="1" colspan="1">Conserved hypothetical protein</td><td valign="top" align="left" rowspan="1" colspan="1"><italic>Ricinus communis</italic></td><td valign="top" align="center" rowspan="1" colspan="1">2.00E-09</td><td valign="top" align="center" rowspan="1" colspan="1">83</td><td valign="top" align="center" rowspan="1" colspan="1">19</td><td valign="top" align="left" rowspan="1" colspan="1"><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002532022.1">XP_002532022.1</ext-link></td><td valign="top" align="center" rowspan="1" colspan="1">0.38</td><td rowspan="1" colspan="1"></td></tr><tr><td valign="top" align="left" rowspan="1" colspan="1"><bold>133_7</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>217</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold>Hypothetical protein SORBIDRAFT_0227s002010</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><italic><bold>Sorghum bicolor</bold></italic></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>2.00E-15</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>97</bold></td><td valign="top" align="center" rowspan="1" colspan="1"><bold>48</bold></td><td valign="top" align="left" rowspan="1" colspan="1"><bold><ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="XP_002489051.1">XP_002489051.1</ext-link></bold></td><td valign="top" align="center" rowspan="1" colspan="1">+</td><td valign="top" align="center" rowspan="1" colspan="1"><bold>0.41</bold></td></tr></tbody></table><table-wrap-foot><p><italic>Data are means of three replicates; Expression ratio, + indicates that TDFs were only isolated from excess B-treated roots. #, Number; BE, B-excess; CK, Control. Functional classification was performed based on the information reported for each sequence by The Gene Ontology (<ext-link ext-link-type="uri" xlink:href="http://amigo1.geneontology.org/cgi-bin/amigo/go.cgi">http://amigo1.geneontology.org/cgi-bin/amigo/go.cgi</ext-link>) and Uniprot (<ext-link ext-link-type="uri" xlink:href="http://www.uniprot.org/">http://www.uniprot.org/</ext-link>). Ratio of BE/CK was obtained by gel image analysis performed with PDQuest version 8.0.1 (Bio-Rad, Hercules, CA, USA). B-excess-responsive TDFs shared by the two citrus species were highlighted in bold</italic>.</p></table-wrap-foot></table-wrap><fig id="F3" position="float"><label>Figure 3</label><caption><p><bold>Functional classification of differentially expressed TDFs under B-excess in <italic><bold>Citrus grandis</bold></italic> (A) and <italic><bold>Citrus sinensis</bold></italic> roots (B) and Venn diagram analysis of differentially expressed TDFs in citrus roots (C)</bold>. Functional classification was performed based on the information reported for each sequence by The Gene Ontology (<ext-link ext-link-type="uri" xlink:href="http://amigo1.geneontology.org/cgi-bin/amigo/go.cgi">http://amigo1.geneontology.</ext-link><ext-link ext-link-type="uri" xlink:href="http://amigo1.geneontology.org/cgi-bin/amigo/go.cgi">org/cgi-bin/amigo/go.cgi</ext-link>) and Uniprot (<ext-link ext-link-type="uri" xlink:href="http://www.uniprot.org/">http://www.uniprot.org/</ext-link>).</p></caption><graphic xlink:href="fpls-07-00898-g0003"></graphic></fig></sec><sec><title>qRT-PCR validation of root differentially expressed TDFs revealed by cDNA-AFLP</title><p>As shown in Figure <xref ref-type="fig" rid="F4">4</xref>, we selected eight and nine TDFs from <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots, respectively, for qRT-PCR analysis in order to examine their expression profiles obtained by cDNA-AFLP. Except for TDFs #137_2 and 53_4 in <italic>C. grandis</italic> roots, the expression patterns of the other TDFs produced by qRT-PCR agreed with the data obtained by cDNA-AFLP.</p><fig id="F4" position="float"><label>Figure 4</label><caption><p><bold>Relative expression levels of TDFs from <italic><bold>Citrus grandis</bold></italic> (A) and <italic><bold>Citrus sinensis</bold></italic> (B) roots. qRT-PCR was run in three biological replicates with two technical replicates</bold>. Citrus <italic>actin</italic> (GU911361.1) was employed as an internal standard, and roots from control plants were used as a reference sample (set as 1). Bars represent means ± <italic>SD</italic>. Different letters above the bars indicate a significant difference at <italic>P</italic> < 0.05.</p></caption><graphic xlink:href="fpls-07-00898-g0004"></graphic></fig></sec><sec><title>Analysis of enzymes for three differentially expressed TDFs</title><p>We assayed the activities of enzymes for three B-excess-responsive TDFs in <italic>C. sinensis</italic> and <italic>C. grandis</italic> roots. The enzyme activities did not match fully with the gene expression levels (Table <xref ref-type="table" rid="T2">2</xref> and Figure <xref ref-type="fig" rid="F5">5</xref>). The discrepancy between the both means that post-modifications (PTMs) might influence these enzyme abundances and their activities. In addition, all these enzymes are composed of two or more isoenzymes, which might be responsible for the discrepancy between gene expression levels and enzyme activities.</p><fig id="F5" position="float"><label>Figure 5</label><caption><p><bold>Effects of B-excess on root CAT (A), POD (B) and LOX (C) activities</bold>. Bars represent means ± <italic>SD</italic> (<italic>n</italic> = 4). Different letters above the bars indicate a significant difference at <italic>P</italic> < 0.05.</p></caption><graphic xlink:href="fpls-07-00898-g0005"></graphic></fig></sec></sec><sec sec-type="discussion" id="s4"><title>Discussion</title><p>We isolated more B-excess-responsive TDFs from <italic>C. grandis</italic> roots than from <italic>C. sinensis</italic>, demonstrating that B-excess affected gene expression more in B-intolerant <italic>C. grandis</italic> roots than in B-tolerant <italic>C. sinensis</italic> roots (Tables <xref ref-type="table" rid="T1">1</xref>, <xref ref-type="table" rid="T2">2</xref>). This agrees with our data that B-excess only lowered P and total soluble protein concentrations in <italic>C. grandis</italic> roots (Figures <xref ref-type="fig" rid="F1">1B,C</xref>) and also agrees with our report that more B-excess-responsive TDFs were obtained from <italic>C. grandis</italic> leaves than from <italic>C. sinensis</italic> leaves (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>). Despite this, great differences existed in B-excess-induced alterations of gene profiles between roots and leaves. For example, most of B-excess-responsive TDFs were identified only from roots or leaves, and only three TDFs with the same genebank ID (i.e., BAD94010.1, NP_564576.1, and XP_003614394.1) were isolated from roots and leaves of <italic>C. sinensis</italic> and <italic>C. grandis</italic>. Moreover, the responses of the three TDFs to B-excess differed between leaves and roots (Table <xref ref-type="table" rid="T2">2</xref>; Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>).</p><sec><title>Effects of B-excess on root carbohydrate and energy metabolism</title><p>As shown in Table <xref ref-type="table" rid="T2">2</xref>, the mRNA levels of three TDFs encoding cytochrome <italic>c</italic> oxidase subunit Vb (TDF #231_1), NADH dehydrogenase subunit 4 (TDF #101_5), and electron transfer flavoprotein-ubiquinone oxidoreductase (TDF #41_2), the components of the mitochondrial respiratory chain, and of two TDFs encoding pyruvate dehydrogenase E1 α subunit (TDF #98_2) and PK (TDF #53_4) involved in glycolysis were lowered in excess B-treated <italic>C. sinensis</italic> roots, implying that respiration was impaired in these roots. Similarly, both <italic>PK</italic> (TDF #53_4) and <italic>phosphoglycerate kinase</italic> (PGK, TDF #185-1) related to glycolysis were inhibited in excess B-treated <italic>C. grandis</italic> roots. Interestingly, the expression of alternative oxidase (AOX, a cyanide-insensitive terminal oxidase) gene (TDF #150_1) was also down-regulated in excess B-treated <italic>C. grandis</italic> roots. Unlike the cytochrome pathway, the AOX pathway, which is cyanide-insensitive and can operate when the cytochrome pathway is restricted, plays a role in inhibiting ROS production in plant mitochondria (Popov et al., <xref rid="B54" ref-type="bibr">1997</xref>; Purvis, <xref rid="B55" ref-type="bibr">1997</xref>; Maxwell et al., <xref rid="B44" ref-type="bibr">1999</xref>; Zidenga et al., <xref rid="B81" ref-type="bibr">2012</xref>). Cytochrome P450s (CytoP450s), a superfamily of monooxygenases, have a key role in (a)biotic stresses. We found that <italic>CytoP45</italic>0 (TDF #137_2) was repressed in excess B-treated <italic>C. grandis</italic> roots, as obtained on B-toxic <italic>Arabidopsis</italic> roots (Aquea et al., <xref rid="B2" ref-type="bibr">2012</xref>). Transgenic potato and tobacco plants expressing <italic>CytoP450</italic> displayed increased monooxygenase activity and enhanced tolerance to oxidative stress after herbicide treatment (Gorinova et al., <xref rid="B22" ref-type="bibr">2005</xref>). Therefore, B-excess-induced down-regulation of <italic>AOX</italic> and <italic>CytoP450</italic> in <italic>C. grandis</italic> roots might contribute to the less B-tolerance of <italic>C. grandis</italic>.</p></sec><sec><title>Effects of B-excess on root cell wall and cytoskeleton metabolism</title><p>Cell wall and cytoskeleton metabolism were altered in excess B-treated <italic>C. sinensis</italic> and <italic>C. grandis</italic> roots, as indicated by B-excess-induced alterations of 10 related genes (i.e., 65_1, 151_2, 37_2, 99_2, 50_1, 26_1, 231_3, 138_1, 249_1, and 253_1; Table <xref ref-type="table" rid="T2">2</xref>). Similar results have been obtained on excess B-treated citrus leaves (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>; Sang et al., <xref rid="B58" ref-type="bibr">2015</xref>). This agrees with the reports that B-toxicity affected citrus root cell walls (Huang et al., <xref rid="B28" ref-type="bibr">2014</xref>) and that B-toxicity disturbed actin filament organization, thus impairing the directional transport of cell wall materials and cell wall construction in <italic>Malus domestica</italic> pollen tubes (Fang et al., <xref rid="B19" ref-type="bibr">2016</xref>). <italic>Root Hair Defective 3</italic> (<italic>RHD3</italic>), a gene encoding a putative GTP-binding protein, is required for actin organization, cell wall biosynthesis, and normal cell expansion in <italic>Arabidopsis</italic> roots (Wang et al., <xref rid="B72" ref-type="bibr">2002</xref>; Hu et al., <xref rid="B27" ref-type="bibr">2003</xref>). Overexpression of <italic>PeRHD</italic> in poplar led to less formation of adventitious roots, better-developed lateral roots, and more and longer root hairs (Xu et al., <xref rid="B75" ref-type="bibr">2012</xref>). B-excess-induced up-regulation of <italic>RHD3</italic> in <italic>C. sinensis</italic> roots might be an adaptive response by maintaining root normal growth and development. By contrast, <italic>villin 4</italic> (TDF #50_1), an actin filament bundling protein gene, was down-regulated in excess B-treated <italic>C. grandis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>). Zhang et al. (<xref rid="B78" ref-type="bibr">2011</xref>) demonstrated that <italic>Arabidopsi</italic>s <italic>villin 4</italic> was required for normal root hair growth through regulating the organization of actin bundles. The down-regulation of <italic>villin 4</italic> implied that B-excess impaired actin organization in <italic>C. grandis</italic> roots, thus affecting the root growth. The differences in Al-induced alterations of root cell wall and cytoskeleton metabolism between the two citrus species might be responsible for the greater decrease in root dry weight in B-toxic <italic>C. grandis</italic> seedlings relative to B-toxic <italic>C. sinensis</italic> ones (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>).</p></sec><sec><title>Effects of B-excess on root lipid metabolism</title><p>We found that all these differentially expressed TDFs (i.e., TDFs #183_2, 249_2, 24_2, 53_3, 129_3, 150_2, 89_2, and 103_6) related to lipid metabolism were inhibited in excess B-treated <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots except for three TDFs (i.e., TDFs #44_1, 9_1, and 42_1; Table <xref ref-type="table" rid="T2">2</xref>), implying that lipid metabolism pathway in these roots was upset. Glycerol-3-phosphate acyltransferase (GPAT), the first enzyme for the biosynthesis of glycerolipids in all organisms, plays crucial roles in basic cellular metabolism. AtGPAT9 is required for the biosynthesis of membrane lipids and oils. A knockout mutant of <italic>AtGPAT9</italic> was homozygous lethal (Shockey et al., <xref rid="B61" ref-type="bibr">2016</xref>). We found that <italic>GPAT9</italic> (TDF #183_2) was down-regulated in excess B-treated <italic>C. grandis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>), suggesting that B-toxicity impaired membrane lipid biosynthesis, thus lowering B-tolerance of <italic>C. grandis</italic>.</p><p>Lipoxygenase (LOX) reactions may initiate the generation of ROS, thus leading to the loss of membranes (Pérez-Gilabert et al., <xref rid="B53" ref-type="bibr">2001</xref>). Inal et al. (<xref rid="B30" ref-type="bibr">2009</xref>) found that B-excess increased malondialdehyde (MDA) level and LOX activity, and silicon (Si) enhanced B-tolerance by decreasing B-excess-induced increases in MDA level and LOX activity in barely. B-excess down-regulated (did not significantly affect) <italic>LOX</italic> expression in <italic>C. grandis</italic> (<italic>C. sinensis</italic>) roots (Table <xref ref-type="table" rid="T2">2</xref>) but increased (did not significantly affect) LOX activity in <italic>C. grandis</italic> (<italic>C. sinensis</italic>) roots (Figure <xref ref-type="fig" rid="F5">5C</xref>). Therefore, the observed higher LOX activity might lower the B-tolerance of <italic>C. grandis</italic>.</p><p>We found that <italic>citrus dioxygenase</italic> (TDF #9_1) was up-regulated in excess B-treated <italic>C. sinensis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>). Alpha-dioxygenase (α-DOX) functions in protecting tissues against oxidative damage and cell death (Tirajoh et al., <xref rid="B66" ref-type="bibr">2005</xref>). Therefore, <italic>citrus dioxygenase</italic> might be involved in the B-tolerance of <italic>C. sinensis</italic>.</p></sec><sec><title>Effects of B-excess on root nucleic acid metabolism</title><p>Boron-toxicity affects nucleic acid metabolic process in higher plants (Kasajima and Fujiwara, <xref rid="B34" ref-type="bibr">2007</xref>; Sakamoto et al., <xref rid="B57" ref-type="bibr">2011</xref>; Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>). As expected, three up- (i.e., TDFs #140_2, 129_1 and 148_1) and five down-regulated (i.e., TDFs #82_1, 28_1, 131_4, 60_1 and 246_1) TDFs, and three up- (i.e., TDFs #79_1, 123_2 and 81_3) and six down-regulated (TDFs #246_1, 195_1, 133_4, 134_1, 142_1, and 252_1) TDFs related to nucleic acid metabolism were isolated from excess B-treated <italic>C. sinensis</italic> and <italic>C. grandis</italic> roots, respectively (Table <xref ref-type="table" rid="T2">2</xref>). Histone deacetylases and histone acetyltransferases, which catalyze histone acetylation and deacetylation, respectively, play a key role in the regulation of gene and genome activity. <italic>Arabidopsis</italic> histone deacetylase 19 (AtHDA19 or AtHD1) plays a role in plant growth and development. Reduction in <italic>AtHDA19</italic> expression by T-DNA insertion or antisense inhibition in <italic>Arabidopsis</italic> caused various developmental abnormalities such as suppression of apical dominance, early senescence, and flower defects (Tian and Chen, <xref rid="B64" ref-type="bibr">2001</xref>; Tian et al., <xref rid="B65" ref-type="bibr">2003</xref>). Recently, Chen et al. (<xref rid="B10" ref-type="bibr">2015</xref>) obtained a total of 86 P-deficiency-responsive genes, which showed lower expression in AtHDA19-RNAi <italic>Arabidopsis</italic> plants relative to 35S:AtHDA19 lines. Kaya et al. (<xref rid="B35" ref-type="bibr">2009</xref>) observed that high-B-treated tomato leaves had lower P concentration, and that supplementary P alleviated high-B-induced decreases in plant growth and fruit yield. B-toxicity decreased P uptake in wheat (Singh et al., <xref rid="B63" ref-type="bibr">1990</xref>). Therefore, excess B-induced up-regulated of <italic>HDA19</italic> (TDF #140_2) in <italic>C. sinensis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>) might be an adaptive response by the maintenances of P homeostasis. This agrees with our report that B-toxicity only reduced P level in <italic>C. grandis</italic> leaves (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>) and roots (Figure <xref ref-type="fig" rid="F1">1B</xref>). Similarly, histone H4 (TDF #129_1) was up-regulated in excess B-treated <italic>C. sinensis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>), which agrees with the reports that <italic>histone H4</italic> was induced in Al-toxic rice roots (Mao et al., <xref rid="B42" ref-type="bibr">2004</xref>) and Al-toxicity increased B level and decreased P level in plant roots, stems, and leaves (Jiang et al., <xref rid="B33" ref-type="bibr">2009a</xref>,<xref rid="B32" ref-type="bibr">b</xref>). Histones H2A, H2B, H3, and H4, the core component of nucleosome, play crucial roles in chromosomal stability, gene regulation, and DNA repair and replication (Turner, <xref rid="B69" ref-type="bibr">2002</xref>; Drury et al., <xref rid="B18" ref-type="bibr">2012</xref>; Zhu et al., <xref rid="B80" ref-type="bibr">2012</xref>). Sakamoto et al. (<xref rid="B57" ref-type="bibr">2011</xref>) demonstrated that plant condensin II played a crucial role in the tolerance of <italic>Arabidopsis</italic> to B-toxicity by alleviating DNA damage. Therefore, the up-regulation of <italic>histone H4</italic> in excess B-treated <italic>C. sinensis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>) might contribute to the B-tolerance of <italic>C. sinensis</italic> by preventing genome from damage resulting from B-excess.</p><p>Ribonucleotide reductase (RNR), an enzyme comprising two R1 and two R2, plays a key role in DNA repair and replication (Chabouté et al., <xref rid="B9" ref-type="bibr">1998</xref>; Wang and Liu, <xref rid="B71" ref-type="bibr">2006</xref>). The down of <italic>RNR1 like protein</italic> (TDF #195_1) in excess B-treated <italic>C. grandis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>) implied that B-toxicity impaired DNA repair and replication, thus lowering the B-tolerance of <italic>C. grandis</italic>. Similarly, the expression levels of <italic>phosphoribosylaminoimidazole carboxylase/AIR carboxylase</italic> (TDF #133_4) involved in nucleotide biosynthesis were down-regulated in excess B-treated <italic>C. grandis</italic> roots.</p></sec><sec><title>Effects of B-excess on root protein and amino acid metabolism</title><p>As shown in Table <xref ref-type="table" rid="T2">2</xref>, we isolated 10 down-regulated (i.e., TDFs #85_2, 132_3, 73_2, 86_1, 112_1, 27_1, 62_1, 118_5, 41_1, and 24_1) and three up-regulated (i.e., TDFs #42_3, 230_1, and 61_1) TDFs involved in protein degradation, two down-regulated TDFs (i.e., TDFs #253_4 and 184_1,) involved in protein folding and stability, five up-regulated (i.e., TDFs #12_1, 121_2, 9_3, 64_2, and 140_1) and six down-regulated (i.e., TDFs #48_1, 246_5, 153_2, 80_2, 38_1, and 14_2) TDFs involved in protein biosynthesis, and four down-regulated TDFs (i.e., TDFs #229_1, 144_2, 5_1, and 229_2) involved in amino acid metabolism. Obviously, protein and amino acid metabolism was impaired in B-toxic citrus roots.</p></sec><sec><title>Effects of B-excess on root stress-related gene expression</title><p>Boron-excess causes the accumulation of ROS, thus resulting in oxidative and membrane damage in plant roots and leaves (Cervilla et al., <xref rid="B7" ref-type="bibr">2007</xref>; Pandey and Archana, <xref rid="B48" ref-type="bibr">2013</xref>; Martínez-Cuenca et al., <xref rid="B43" ref-type="bibr">2015</xref>). An antioxidant response has been suggested to abate B-toxic damage in some higher plants (Gunes et al., <xref rid="B23" ref-type="bibr">2006</xref>). Here, we isolated one up-regulated <italic>homogentisate phytyltransferase 1</italic> (<italic>HPT1</italic>; TDF #182_3) from excess B-treated <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>). HPT catalyzes the committed step in the biosynthesis of tocopherol (vitamin E). Collakova and DellaPenna (<xref rid="B14" ref-type="bibr">2003</xref>) showed that transgenic <italic>Arabidopsis</italic> plants overexpressing <italic>HPT1</italic> had an elevated total tocopherol level in seed and leaves. The up-regulation of <italic>HPT1</italic> agrees with the increased requirement for the detoxification of ROS in excess B-treated <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots. In addition, <italic>Obg-like ATPase 1</italic> (<italic>YchF1</italic>; TDF #64_1), a negative regulator of the antioxidant response (Cheung et al., <xref rid="B13" ref-type="bibr">2013</xref>), was down-regulated in excess B-treated <italic>C. grandis</italic> roots. However, <italic>POD</italic> (TDF #99_3) in <italic>C. grandis</italic> roots and <italic>CAT</italic> (TDF #231_5) in <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots were down-regulated by B-excess. Enzyme activity analysis showed that B-excess lowered CAT and POD activities in <italic>C. grandis</italic> roots but did not significantly affect their activities in <italic>C. sinensis</italic> roots (Figures <xref ref-type="fig" rid="F5">5A,B</xref>). Besides, the expression level of an antioxidant protein gene (Xu and Møller, <xref rid="B77" ref-type="bibr">2010</xref>; Xu et al., <xref rid="B76" ref-type="bibr">2010</xref>), <italic>DJ-1 family protein</italic> (TDF #30_1) was reduced in excess B-treated <italic>C. grandis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>). Obviously, excess B-treated <italic>C. sinensis</italic> roots kept higher antioxidant capacity than excess B-treated <italic>C. grandis</italic> roots.</p><p>Two putative senescence-associated protein genes (i.e., TDFs #131_1 and 17_1) were induced by B-excess in <italic>C. grandis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>), suggesting that senescence was accelerated in these roots. This agrees with our report that B-excess affected root growth more in <italic>C. grandis</italic> than in <italic>C. sinensis</italic> seedlings (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>). Similarly, autophagic cell death might be accelerated in excess B-treated <italic>C. grandis</italic> roots, as indicated by the enhanced expression level of <italic>WD repeat phosphoinositide-interacting-like protein</italic> (<italic>WILP</italic>; TDF #104_3) in these roots (Table <xref ref-type="table" rid="T2">2</xref>). This agrees with our report that B-excess led to cell death in <italic>C. grandis</italic> roots (Huang et al., <xref rid="B28" ref-type="bibr">2014</xref>).</p></sec><sec><title>Effects of B-excess on root signal transduction</title><p>Calcium (Ca) signaling plays vital roles in plant responses to various stresses (Knight, <xref rid="B38" ref-type="bibr">2000</xref>). Ca can alleviate B-toxicity and high-B inhibits Ca uptake in higher plants (Singh et al., <xref rid="B63" ref-type="bibr">1990</xref>; Turan et al., <xref rid="B68" ref-type="bibr">2009</xref>; Siddiqui et al., <xref rid="B62" ref-type="bibr">2013</xref>). Hence, genes related to Ca<sup>2+</sup> signal transduction should be altered in excess B-treated citrus roots. As expected, we isolated one up-regulated TDF: <italic>ATP/GTP/Ca</italic><sup>2+</sup><italic>binding protein</italic> [also called as <italic>mitochondrial Rho GTPase</italic> (<italic>MIRO</italic>)] from B-toxic <italic>C. sinensis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>). Jayasekaran et al. (<xref rid="B31" ref-type="bibr">2006</xref>) found that the transcript level of <italic>MIRO2</italic> was elevated in ABA- and salt-treated <italic>Arabidopsis</italic> plants, and that the knock-out mutants of <italic>MIRO2</italic> were less tolerance to ABA, salt, and osmotic treatments than the wild-type. By contrast, we only isolated three down-regulated TDFs (i.e., TDFs #80_3, 67-2, and 35_2) involved in Ca signals from B-toxic <italic>C. grandis</italic> roots. Therefore, Ca<sup>2+</sup>-mediated signal played a role in the tolerance of citrus plants to B-toxicity.</p></sec><sec><title>Effects of B-excess on transport processes in root cells</title><p>As shown in Table <xref ref-type="table" rid="T2">2</xref>, the mRNA levels of all the 13 differentially expressed TDFs related to cellular transport were reduced in excess B-treated <italic>C. grandis</italic> and <italic>C. sinensis</italic> roots except for one up-regulated <italic>H</italic><sup>+</sup><italic>-ATPase 4</italic> (<italic>AHA4</italic>, TDF #251_2), indicating that the transport of some substances (i.e., ion and proteins) was altered in these roots. This disagrees with our report that most of the differentially expressed genes involved in cellular transport were up-regulated in excess B-treated <italic>C. grandis</italic> leaves and three up- and three down-regulated genes were isolated from excess B-treated <italic>C. sinensis</italic> leaves (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>).</p><p><italic>ABC transporter I family member 20</italic> (<italic>ABCI20</italic>; TDF #183_2) was down-regulated in excess B-treated <italic>C. grandis</italic> roots (Table <xref ref-type="table" rid="T2">2</xref>). This agrees with our report that <italic>ABC1 family protein</italic> (AT5g24810) was repressed in high-B-treated <italic>C. grandis</italic> leaves (Guo et al., <xref rid="B24" ref-type="bibr">2014</xref>). In eukaryotic organisms, ABC transporters have been shown to play a role in the efflux (extracellular or intracellular into the vacuoles) of potentially toxic compounds (i.e., heavy metals, alkaloids, and organic anions; Gaedeke et al., <xref rid="B21" ref-type="bibr">2001</xref>). Transgenic <italic>Arabidopsis</italic> plants overexpressing <italic>AtPDR8</italic> encoding an ABC transporter displayed higher Cd- and Pb-tolerance accompanied by less accumulation of Cd in the shoots or roots. By contrast, mutants were less tolerance to both the metals accompanied by more accumulation of Cd (Kim et al., <xref rid="B37" ref-type="bibr">2007</xref>). Therefore, the down-regulation of <italic>ABCI20</italic> and <italic>ABC1 family protein</italic> in roots and leaves might reduce the efflux of B from the PM, thus increasing free B level and lowering <italic>C. grandis</italic> B-tolerance. Interestingly, middle leaves from excess B-treated <italic>C. grandis</italic> seedlings displayed more free B and less bound B than those from <italic>C. sinensis</italic>, but free B and bound B in excess B-treated roots did not significantly differ between the two citrus species (Huang et al., <xref rid="B28" ref-type="bibr">2014</xref>). Previous studies showed that <italic>C. sinensis</italic> had defective nodulin-26-like intrinsic protein 2 (NIP2) gene, which is involved in influx of Si, B, and other metalloids (Schnurbusch et al., <xref rid="B59" ref-type="bibr">2010</xref>; Deshmukh et al., <xref rid="B16" ref-type="bibr">2015</xref>). Therefore, NIP2 could be involved in compartmentation of B differently among this species. However, B-excess did not alter <italic>NIP2</italic> expression in citrus roots, suggesting that other transporters played a role in citrus compartmentation of B or that <italic>NIP2</italic> was regulated at the post-translational level.</p></sec></sec><sec sec-type="conclusions" id="s5"><title>Conclusions</title><p>We used cDNA-AFLP to investigate comparatively the effects of B-excess on gene profiles in B-tolerant <italic>C. sinensis</italic> and B-intolerant <italic>C. grandis</italic> roots, and isolated 20 up- and 52 down-regulated, and 44 up- and 66 down-regulated TDFs from excess B-treated <italic>C. sinensis</italic> and <italic>C. grandis</italic> roots, respectively, thereby demonstrating that gene expression was less affected in the former than in the latter. Besides, B-excess only lowered P and total soluble protein concentrations in <italic>C. grandis</italic> roots. Obviously, <italic>C. sinensis</italic> seedlings had higher B-tolerance than <italic>C. grandis</italic> ones. Our results suggested that the following several aspects led to the difference in the B-tolerance between the both, including: (a) <italic>AOX</italic> and <italic>CytoP450</italic> were down-regulated only in excess B-treated <italic>C. grandis</italic> roots; (b) B-excess up-regulated <italic>RHD3</italic> in <italic>C. sinensis</italic> roots and down-regulated <italic>villin4</italic> in <italic>C. grandis</italic> roots, and accordingly, root dry weight was less reduced by B-excess in the former; (c) antioxidant systems were impaired in excess B-treated <italic>C. grandis</italic> roots, hence accelerating root senescence; (d) TDFs involved in Ca<sup>2+</sup> signals were down-regulated in excess B-treated <italic>C. grandis</italic> roots and up-regulated in excess B-treated <italic>C. sinensis</italic> roots. In addition, B-excess-responsive genes related to lipid (i.e., <italic>GPAT9</italic> and <italic>citrus dioxygenase</italic>) and nucleic acid (i.e., <italic>histone deacetylase 19, histone 4</italic>, and <italic>RNR1 like protein</italic>) metabolisms also possibly contributed to the difference in the B-tolerance between the both. Our data provided some new clues for our understanding of the molecular mechanisms on citrus B-toxicity and B-tolerance.</p></sec><sec id="s6"><title>Author contributions</title><p>PG carried out most of the experiments and drafted the manuscript. YQ participated in the design of the study. LY participated in the design of the study and coordination. XY participated in the measurement of B and P. JH participated in the statistical analysis. LC designed and directed the study and revised the manuscript. All authors have read and approved the final manuscript.</p></sec><sec id="s7"><title>Funding</title><p>Our work was funded by the earmarked fund for China Agriculture Research System (No. CARS-27).</p><sec><title>Conflict of interest statement</title><p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p></sec></sec></body><back><sec sec-type="supplementary-material" id="s8"><title>Supplementary material</title><p>The Supplementary Material for this article can be found online at: <ext-link ext-link-type="uri" xlink:href="http://journal.frontiersin.org/article/10.3389/fpls.2016.00898">http://journal.frontiersin.org/article/10.3389/fpls.2016.00898</ext-link></p><supplementary-material content-type="local-data" id="SM1"><media xlink:href="DataSheet1.DOC"><caption><p>Click here for additional data file.</p></caption></media></supplementary-material></sec><ref-list><title>References</title><ref id="B1"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ames</surname><given-names>B. N.</given-names></name></person-group> (<year>1966</year>). <article-title>Assay of inorganic phosphate, total phosphate and phosphatase</article-title>. <source>Meth. Enzymol.</source><volume>8</volume>, <fpage>115</fpage>–<lpage>118</lpage>. <pub-id pub-id-type="doi">10.1016/0076-6879(66)08014-5</pub-id></mixed-citation></ref><ref id="B2"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Aquea</surname><given-names>F.</given-names></name><name><surname>Federici</surname><given-names>F.</given-names></name><name><surname>Moscoso</surname><given-names>C.</given-names></name><name><surname>Vega</surname><given-names>A.</given-names></name><name><surname>Jullian</surname><given-names>P.</given-names></name><name><surname>Haseloff</surname><given-names>J.</given-names></name><etal></etal></person-group>. (<year>2012</year>). <article-title>A molecular framework for the inhibition of <italic>Arabidopsis</italic> root growth in response to boron toxicity</article-title>. <source>Plant Cell Environ.</source><volume>35</volume>, <fpage>719</fpage>–<lpage>734</lpage>. <pub-id pub-id-type="doi">10.1111/j.1365-3040.2011.02446.x</pub-id><pub-id pub-id-type="pmid">21988710</pub-id></mixed-citation></ref><ref id="B3"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Axelrod</surname><given-names>B.</given-names></name><name><surname>Cheesbrough</surname><given-names>T. M.</given-names></name><name><surname>Laakso</surname><given-names>S.</given-names></name></person-group> (<year>1981</year>). <article-title>Lipoxygenase from soybeans</article-title>. <source>Meth. Enzymol.</source><volume>71</volume>, <fpage>441</fpage>–<lpage>451</lpage>. <pub-id pub-id-type="doi">10.1016/0076-6879(81)71055-3</pub-id></mixed-citation></ref><ref id="B4"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ayvaz</surname><given-names>M.</given-names></name><name><surname>Guven</surname><given-names>A.</given-names></name><name><surname>Fagerstedt</surname><given-names>K.</given-names></name></person-group> (<year>2015</year>). <article-title>Does excess boron affect hormone levels of potato cultivars?</article-title><source>Biotechnol. Biotec. Eq.</source><volume>29</volume>, <fpage>887</fpage>–<lpage>891</lpage>. <pub-id pub-id-type="doi">10.1080/13102818.2015.1053411</pub-id></mixed-citation></ref><ref id="B5"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ayvaz</surname><given-names>M.</given-names></name><name><surname>Koyuncu</surname><given-names>M.</given-names></name><name><surname>Guven</surname><given-names>A.</given-names></name><name><surname>Fagerstedt</surname><given-names>K. V.</given-names></name></person-group> (<year>2012</year>). <article-title>Does boron affect hormone levels of barley cultivars?</article-title><source>Eur. Asian J. Biosci.</source><volume>6</volume>, <fpage>113</fpage>–<lpage>120</lpage>. <pub-id pub-id-type="doi">10.5053/ejobios.2012.6.0.14</pub-id></mixed-citation></ref><ref id="B6"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Bradford</surname><given-names>M. M.</given-names></name></person-group> (<year>1976</year>). <article-title>A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding</article-title>. <source>Anal. Biochem.</source><volume>72</volume>, <fpage>248</fpage>–<lpage>254</lpage>. <pub-id pub-id-type="doi">10.1016/0003-2697(76)90527-3</pub-id><pub-id pub-id-type="pmid">942051</pub-id></mixed-citation></ref><ref id="B7"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cervilla</surname><given-names>L. M.</given-names></name><name><surname>Blasco</surname><given-names>B.</given-names></name><name><surname>Ríos</surname><given-names>J.</given-names></name><name><surname>Romero</surname><given-names>L.</given-names></name><name><surname>Ruiz</surname><given-names>J.</given-names></name></person-group> (<year>2007</year>). <article-title>Oxidative stress and antioxidants in tomato (<italic>Solanum lycopericum</italic>) plants subjected to boron toxicity</article-title>. <source>Ann. Bot.</source><volume>100</volume>, <fpage>747</fpage>–<lpage>756</lpage>. <pub-id pub-id-type="doi">10.1093/aob/mcm156</pub-id><pub-id pub-id-type="pmid">17660516</pub-id></mixed-citation></ref><ref id="B8"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cervilla</surname><given-names>L. M.</given-names></name><name><surname>Blasco</surname><given-names>B.</given-names></name><name><surname>Ríos</surname><given-names>J. J.</given-names></name><name><surname>Rosales</surname><given-names>M. A.</given-names></name><name><surname>Rubio-Wilhelmi</surname><given-names>M. M.</given-names></name><name><surname>Sánchez-Rodríguez</surname><given-names>E.</given-names></name><etal></etal></person-group>. (<year>2009</year>). <article-title>Response of nitrogen metabolism to boron toxicity in tomato plants</article-title>. <source>Plant Biol.</source><volume>11</volume>, <fpage>671</fpage>–<lpage>677</lpage>. <pub-id pub-id-type="doi">10.1111/j.1438-8677.2008.00167.x</pub-id><pub-id pub-id-type="pmid">19689774</pub-id></mixed-citation></ref><ref id="B9"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chabouté</surname><given-names>M. E.</given-names></name><name><surname>Combettes</surname><given-names>B.</given-names></name><name><surname>Clément</surname><given-names>B.</given-names></name><name><surname>Gigot</surname><given-names>C.</given-names></name><name><surname>Philipps</surname><given-names>G.</given-names></name></person-group> (<year>1998</year>). <article-title>Molecular characterization of tobacco ribonucleotide reductase RNR1 and RNR2 cDNAs and cell cycle-regulated expression in synchronized plant cells</article-title>. <source>Plant Mol. Biol.</source><volume>38</volume>, <fpage>797</fpage>–<lpage>806</lpage>. <pub-id pub-id-type="doi">10.1023/A:1006083318906</pub-id><pub-id pub-id-type="pmid">9862497</pub-id></mixed-citation></ref><ref id="B10"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>C. Y.</given-names></name><name><surname>Wu</surname><given-names>K.</given-names></name><name><surname>Schmidt</surname><given-names>W.</given-names></name></person-group> (<year>2015</year>). <article-title>The histone deacetylase HDA19 controls root cell elongation and modulates a subset of phosphate starvation responses in <italic>Arabidopsis</italic></article-title>. <source>Sci. Rep.</source><volume>5</volume>:<fpage>15708</fpage>. <pub-id pub-id-type="doi">10.1038/srep15708</pub-id><pub-id pub-id-type="pmid">26508133</pub-id></mixed-citation></ref><ref id="B11"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>L. S.</given-names></name><name><surname>Han</surname><given-names>S.</given-names></name><name><surname>Qi</surname><given-names>Y. P.</given-names></name><name><surname>Yang</surname><given-names>L. T.</given-names></name></person-group> (<year>2012</year>). <article-title>Boron stresses and tolerance in citrus</article-title>. <source>Afr. J. Biotech.</source><volume>11</volume>, <fpage>5961</fpage>–<lpage>5969</lpage>. <pub-id pub-id-type="doi">10.5897/AJBX11.073</pub-id></mixed-citation></ref><ref id="B12"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname><given-names>M.</given-names></name><name><surname>Mishra</surname><given-names>S.</given-names></name><name><surname>Heckathorn</surname><given-names>S. A.</given-names></name><name><surname>Frantz</surname><given-names>J. M.</given-names></name><name><surname>Krause</surname><given-names>C.</given-names></name></person-group> (<year>2014</year>). <article-title>Proteomic analysis of <italic>Arabidopsis thaliana</italic> leaves in response to acute boron deficiency and toxicity reveals effects on photosynthesis, carbohydrate metabolism, and protein synthesis</article-title>. <source>J. Plant Physiol.</source><volume>171</volume>, <fpage>235</fpage>–<lpage>242</lpage>. <pub-id pub-id-type="doi">10.1016/j.jplph.2013.07.008</pub-id><pub-id pub-id-type="pmid">23988561</pub-id></mixed-citation></ref><ref id="B13"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Cheung</surname><given-names>M. Y.</given-names></name><name><surname>Li</surname><given-names>M. W.</given-names></name><name><surname>Yung</surname><given-names>Y. L.</given-names></name><name><surname>Wen</surname><given-names>C. Q.</given-names></name><name><surname>Lam</surname><given-names>H. M.</given-names></name></person-group> (<year>2013</year>). <article-title>The unconventional P-loop NTPase OsYchF1 and its regulator OsGAP1 play opposite roles in salinity stress tolerance</article-title>. <source>Plant Cell Environ.</source><volume>36</volume>, <fpage>2008</fpage>–<lpage>2020</lpage>. <pub-id pub-id-type="doi">10.1111/pce.12108</pub-id><pub-id pub-id-type="pmid">23550829</pub-id></mixed-citation></ref><ref id="B14"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Collakova</surname><given-names>E.</given-names></name><name><surname>DellaPenna</surname><given-names>D.</given-names></name></person-group> (<year>2003</year>). <article-title>Homogentisate phytyltransferase activity is limiting for tocopherol biosynthesis in <italic>Arabidopsis</italic></article-title>. <source>Plant Physiol.</source><volume>131</volume>, <fpage>632</fpage>–<lpage>642</lpage>. <pub-id pub-id-type="doi">10.1104/pp.015222</pub-id><pub-id pub-id-type="pmid">12586887</pub-id></mixed-citation></ref><ref id="B15"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Craciun</surname><given-names>A. R.</given-names></name><name><surname>Courbot</surname><given-names>M.</given-names></name><name><surname>Bourgis</surname><given-names>F.</given-names></name><name><surname>Salis</surname><given-names>P.</given-names></name><name><surname>Saumitou-Laprade</surname><given-names>P.</given-names></name><name><surname>Verbruggen</surname><given-names>N.</given-names></name></person-group> (<year>2006</year>). <article-title>Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator <italic>Arabidopsis halleri</italic> and <italic>Arabidopsis lyrata</italic> ssp. petraea</article-title>. <source>J. Exp. Bot.</source><volume>57</volume>, <fpage>2967</fpage>–<lpage>2983</lpage>. <pub-id pub-id-type="doi">10.1093/jxb/erl062</pub-id><pub-id pub-id-type="pmid">16916885</pub-id></mixed-citation></ref><ref id="B16"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Deshmukh</surname><given-names>R. K.</given-names></name><name><surname>Vivancos</surname><given-names>J.</given-names></name><name><surname>Ramakrishnan</surname><given-names>G.</given-names></name><name><surname>Guérin</surname><given-names>V.</given-names></name><name><surname>Carpentier</surname><given-names>G.</given-names></name><name><surname>Sonah</surname><given-names>H.</given-names></name><etal></etal></person-group>. (<year>2015</year>). <article-title>A precise spacing between the NPA domains of aquaporins is essential for silicon permeability in plants</article-title>. <source>Plant J.</source><volume>83</volume>, <fpage>489</fpage>–<lpage>500</lpage>. <pub-id pub-id-type="doi">10.1111/tpj.12904</pub-id><pub-id pub-id-type="pmid">26095507</pub-id></mixed-citation></ref><ref id="B17"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Ditt</surname><given-names>R. F.</given-names></name><name><surname>Nester</surname><given-names>E. W.</given-names></name><name><surname>Comai</surname><given-names>L.</given-names></name></person-group> (<year>2001</year>). <article-title>Plant gene expression response to <italic>Agrobacterium tumefaciens</italic></article-title>. <source>Proc. Natl. Acad. Sci. U.S.A.</source><volume>98</volume>, <fpage>10954</fpage>–<lpage>10959</lpage>. <pub-id pub-id-type="doi">10.1073/pnas.191383498</pub-id><pub-id pub-id-type="pmid">11535836</pub-id></mixed-citation></ref><ref id="B18"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Drury</surname><given-names>G. E.</given-names></name><name><surname>Dowle</surname><given-names>A. A.</given-names></name><name><surname>Ashford</surname><given-names>D. A.</given-names></name><name><surname>Waterworth</surname><given-names>W. M.</given-names></name><name><surname>Thomas</surname><given-names>J.</given-names></name><name><surname>West</surname><given-names>C. E.</given-names></name></person-group> (<year>2012</year>). <article-title>Dynamics of plant histone modifications in response to DNA damage</article-title>. <source>Biochem. J.</source><volume>445</volume>, <fpage>393</fpage>–<lpage>401</lpage>. <pub-id pub-id-type="doi">10.1042/BJ20111956</pub-id><pub-id pub-id-type="pmid">22574698</pub-id></mixed-citation></ref><ref id="B19"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fang</surname><given-names>K.</given-names></name><name><surname>Zhang</surname><given-names>W.</given-names></name><name><surname>Xing</surname><given-names>Y.</given-names></name><name><surname>Zhang</surname><given-names>Q.</given-names></name><name><surname>Yang</surname><given-names>L.</given-names></name><name><surname>Cao</surname><given-names>Q.</given-names></name><etal></etal></person-group>. (<year>2016</year>). <article-title>Boron toxicity causes multiple effects on <italic>Malus domestica</italic> pollen tube growth</article-title>. <source>Front. Plant Sci.</source><volume>7</volume>:<issue>208</issue>. <pub-id pub-id-type="doi">10.3389/fpls.2016.00208</pub-id><pub-id pub-id-type="pmid">26955377</pub-id></mixed-citation></ref><ref id="B20"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Fusco</surname><given-names>N.</given-names></name><name><surname>Micheletto</surname><given-names>L.</given-names></name><name><surname>DalCorso</surname><given-names>G.</given-names></name><name><surname>Borgato</surname><given-names>L.</given-names></name><name><surname>Furini</surname><given-names>A.</given-names></name></person-group> (<year>2005</year>). <article-title>Identification of cadmium-regulated genes by cDNA-AFLP in the heavy metal accumulator <italic>Brassica juncea</italic> L</article-title>. <source>J. Exp. Bot.</source><volume>56</volume>, <fpage>3017</fpage>–<lpage>3027</lpage>. <pub-id pub-id-type="doi">10.1093/jxb/eri299</pub-id><pub-id pub-id-type="pmid">16216843</pub-id></mixed-citation></ref><ref id="B21"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gaedeke</surname><given-names>N.</given-names></name><name><surname>Klein</surname><given-names>M.</given-names></name><name><surname>Kolukisaoglu</surname><given-names>U.</given-names></name><name><surname>Forestier</surname><given-names>C.</given-names></name><name><surname>Müller</surname><given-names>A.</given-names></name><name><surname>Ansorge</surname><given-names>M.</given-names></name><etal></etal></person-group>. (<year>2001</year>). <article-title>The <italic>Arabidopsis thaliana</italic> ABC transporter AtMRP5 controls root development and stomata movement</article-title>. <source>EMBO J.</source><volume>20</volume>, <fpage>1875</fpage>–<lpage>1887</lpage>. <pub-id pub-id-type="doi">10.1093/emboj/20.8.1875</pub-id><pub-id pub-id-type="pmid">11296221</pub-id></mixed-citation></ref><ref id="B22"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gorinova</surname><given-names>N.</given-names></name><name><surname>Nedkovska</surname><given-names>M.</given-names></name><name><surname>Atanassov</surname><given-names>A.</given-names></name></person-group> (<year>2005</year>). <article-title>Cytochrome P450 monooxygenases as a tool for metabolization herbicides in plants</article-title>. <source>Biotechnol. Biotechnol. Equip</source>. <volume>19</volume>, <fpage>105</fpage>–<lpage>115</lpage>. Special Issue. <pub-id pub-id-type="doi">10.1080/13102818.2005.10817290</pub-id></mixed-citation></ref><ref id="B23"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Gunes</surname><given-names>A.</given-names></name><name><surname>Soylemezoglu</surname><given-names>G.</given-names></name><name><surname>Inal</surname><given-names>A.</given-names></name><name><surname>Bagci</surname><given-names>E. G.</given-names></name><name><surname>Coban</surname><given-names>S.</given-names></name><name><surname>Sahin</surname><given-names>O.</given-names></name></person-group> (<year>2006</year>). <article-title>Antioxidant and stomatal responses of grapevine (<italic>Vitis vinifera</italic> L.) to boron toxicity</article-title>. <source>Sci. Hort.</source><volume>110</volume>, <fpage>279</fpage>–<lpage>284</lpage>. <pub-id pub-id-type="doi">10.1016/j.scienta.2006.07.014</pub-id></mixed-citation></ref><ref id="B24"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Guo</surname><given-names>P.</given-names></name><name><surname>Qi</surname><given-names>Y. P.</given-names></name><name><surname>Yang</surname><given-names>L. T.</given-names></name><name><surname>Ye</surname><given-names>X.</given-names></name><name><surname>Jiang</surname><given-names>H. X.</given-names></name><name><surname>Huang</surname><given-names>J. H.</given-names></name><etal></etal></person-group>. (<year>2014</year>). <article-title>cDNA-AFLP analysis reveals the adaptive responses of citrus to long-term boron-toxicity</article-title>. <source>BMC Plant Biol.</source><volume>14</volume>:<fpage>284</fpage>. <pub-id pub-id-type="doi">10.1186/s12870-014-0284-5</pub-id><pub-id pub-id-type="pmid">25348611</pub-id></mixed-citation></ref><ref id="B25"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Han</surname><given-names>S.</given-names></name><name><surname>Tang</surname><given-names>N.</given-names></name><name><surname>Jiang</surname><given-names>H. X.</given-names></name><name><surname>Yang</surname><given-names>L. T.</given-names></name><name><surname>Li</surname><given-names>Y.</given-names></name><name><surname>Chen</surname><given-names>L. S.</given-names></name></person-group> (<year>2009</year>). <article-title>CO<sub>2</sub> assimilation, photosystem II photochemistry, carbohydrate metabolism and antioxidant system of citrus leaves in response to boron stress</article-title>. <source>Plant Sci.</source><volume>176</volume>, <fpage>143</fpage>–<lpage>153</lpage>. <pub-id pub-id-type="doi">10.1016/j.plantsci.2008.10.004</pub-id></mixed-citation></ref><ref id="B26"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hassan</surname><given-names>M.</given-names></name><name><surname>Oldach</surname><given-names>K.</given-names></name><name><surname>Baumann</surname><given-names>U.</given-names></name><name><surname>Langridge</surname><given-names>P.</given-names></name><name><surname>Sutton</surname><given-names>T.</given-names></name></person-group> (<year>2010</year>). <article-title>Genes mapping to boron tolerance QTL in barley identified by suppression subtractive hybridization</article-title>. <source>Plant Cell Environ.</source><volume>33</volume>, <fpage>188</fpage>–<lpage>198</lpage>. <pub-id pub-id-type="doi">10.1111/j.1365-3040.2009.02069.x</pub-id><pub-id pub-id-type="pmid">19906153</pub-id></mixed-citation></ref><ref id="B27"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Hu</surname><given-names>Y.</given-names></name><name><surname>Zhang</surname><given-names>R. G.</given-names></name><name><surname>Morriison</surname><given-names>W. H.</given-names><suffix>3rd</suffix></name><name><surname>Ye</surname><given-names>Z. H.</given-names></name></person-group> (<year>2003</year>). <article-title>The <italic>Arabidopsis RHD3</italic> gene is required for cell wall biosynthesis and actin organization</article-title>. <source>Planta</source><volume>217</volume>, <fpage>912</fpage>–<lpage>921</lpage>. <pub-id pub-id-type="doi">10.1007/s00425-003-1067-7</pub-id><pub-id pub-id-type="pmid">12844267</pub-id></mixed-citation></ref><ref id="B28"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Huang</surname><given-names>J. H.</given-names></name><name><surname>Cai</surname><given-names>Z. J.</given-names></name><name><surname>Wen</surname><given-names>S. X.</given-names></name><name><surname>Guo</surname><given-names>P.</given-names></name><name><surname>Ye</surname><given-names>X.</given-names></name><name><surname>Lin</surname><given-names>G. Z.</given-names></name><etal></etal></person-group> (<year>2014</year>). <article-title>Effects of boron toxicity on root and leaf anatomy in two citrus species differing in boron tolerance</article-title>. <source>Trees Struct. Funct.</source><volume>28</volume>, <fpage>1653</fpage>–<lpage>1666</lpage>. <pub-id pub-id-type="doi">10.1007/s00468-014-1075-1</pub-id></mixed-citation></ref><ref id="B29"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Huang</surname><given-names>J. H.</given-names></name><name><surname>Qi</surname><given-names>Y. P.</given-names></name><name><surname>Wen</surname><given-names>S. X.</given-names></name><name><surname>Guo</surname><given-names>P.</given-names></name><name><surname>Chen</surname><given-names>X. M.</given-names></name><name><surname>Chen</surname><given-names>L. S.</given-names></name></person-group> (<year>2016</year>). <article-title>Illumina microRNA profiles reveal the involvement of miR397a in citrus adaptation to long-term boron toxicity via modulating secondary cell-wall biosynthesis</article-title>. <source>Sci. Rep.</source><volume>6</volume>:<fpage>22900</fpage>. <pub-id pub-id-type="doi">10.1038/srep22900</pub-id><pub-id pub-id-type="pmid">26962011</pub-id></mixed-citation></ref><ref id="B30"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Inal</surname><given-names>A.</given-names></name><name><surname>Pilbeam</surname><given-names>D. J.</given-names></name><name><surname>Gunes</surname><given-names>A.</given-names></name></person-group> (<year>2009</year>). <article-title>Silicon increases tolerance to boron toxicity and reduces oxidative damage in barley</article-title>. <source>J. Plant Nutr.</source><volume>32</volume>, <fpage>112</fpage>–<lpage>128</lpage>. <pub-id pub-id-type="doi">10.1080/01904160802533767</pub-id></mixed-citation></ref><ref id="B31"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jayasekaran</surname><given-names>K.</given-names></name><name><surname>Kim</surname><given-names>K. N.</given-names></name><name><surname>Vivekanandan</surname><given-names>M.</given-names></name><name><surname>Shin</surname><given-names>J. S.</given-names></name><name><surname>Ok</surname><given-names>S. H.</given-names></name></person-group> (<year>2006</year>). <article-title>Novel calcium-binding GTPase (AtCBG) involved in ABA-mediated salt stress signaling in <italic>Arabidopsis</italic></article-title>. <source>Plant Cell Rep.</source><volume>25</volume>, <fpage>1255</fpage>–<lpage>1262</lpage>. <pub-id pub-id-type="doi">10.1007/s00299-006-0195-5</pub-id><pub-id pub-id-type="pmid">16832621</pub-id></mixed-citation></ref><ref id="B32"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jiang</surname><given-names>H. X.</given-names></name><name><surname>Tang</surname><given-names>N.</given-names></name><name><surname>Zheng</surname><given-names>J. G.</given-names></name><name><surname>Chen</surname><given-names>L. S.</given-names></name></person-group> (<year>2009b</year>). <article-title>Antagonistic actions of boron against inhibitory effects of aluminum toxicity on growth, CO<sub>2</sub> assimilation, ribulose-1,5-bisphosphate carboxylase/oxygenase, and photosynthetic electron transport probed by the JIP-test, of <italic>Citrus grandis</italic> seedlings</article-title>. <source>BMC Plant Biol.</source><volume>9</volume>:<fpage>102</fpage>. <pub-id pub-id-type="doi">10.1186/1471-2229-9-102</pub-id><pub-id pub-id-type="pmid">19646270</pub-id></mixed-citation></ref><ref id="B33"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Jiang</surname><given-names>H. X.</given-names></name><name><surname>Tang</surname><given-names>N.</given-names></name><name><surname>Zheng</surname><given-names>J. G.</given-names></name><name><surname>Li</surname><given-names>Y.</given-names></name><name><surname>Chen</surname><given-names>L. S.</given-names></name></person-group> (<year>2009a</year>). <article-title>Phosphorus alleviates aluminum-induced inhibition of growth and photosynthesis in <italic>Citrus grandis</italic> seedlings</article-title>. <source>Physiol. Plant.</source><volume>137</volume>, <fpage>298</fpage>–<lpage>311</lpage>. <pub-id pub-id-type="doi">10.1111/j.1399-3054.2009.01288.x</pub-id><pub-id pub-id-type="pmid">19832942</pub-id></mixed-citation></ref><ref id="B34"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kasajima</surname><given-names>I.</given-names></name><name><surname>Fujiwara</surname><given-names>T.</given-names></name></person-group> (<year>2007</year>). <article-title>Identification of novel <italic>Arabidopsis thaliana</italic> genes which are induced by high levels of boron</article-title>. <source>Plant Biotechnol.</source><volume>24</volume>, <fpage>355</fpage>–<lpage>360</lpage>. <pub-id pub-id-type="doi">10.5511/plantbiotechnology.24.355</pub-id></mixed-citation></ref><ref id="B35"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kaya</surname><given-names>C.</given-names></name><name><surname>Tuna</surname><given-names>A. L.</given-names></name><name><surname>Dikilitas</surname><given-names>M.</given-names></name><name><surname>Ashraf</surname><given-names>M.</given-names></name><name><surname>Koskeroglu</surname><given-names>S.</given-names></name><name><surname>Guneri</surname><given-names>M.</given-names></name></person-group> (<year>2009</year>). <article-title>Supplementary phosphorus can alleviate boron toxicity in tomato</article-title>. <source>Sci. Hort.</source><volume>121</volume>, <fpage>284</fpage>–<lpage>288</lpage>. <pub-id pub-id-type="doi">10.1016/j.scienta.2009.02.011</pub-id></mixed-citation></ref><ref id="B36"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Keles</surname><given-names>Y.</given-names></name><name><surname>Oncel</surname><given-names>I.</given-names></name><name><surname>Yenice</surname><given-names>N.</given-names></name></person-group> (<year>2004</year>). <article-title>Relationship between boron content and antioxidant compounds in citrus leaves taken from fields with different water sources</article-title>. <source>Plant Soil</source><volume>265</volume>, <fpage>343</fpage>–<lpage>353</lpage>. <pub-id pub-id-type="doi">10.1007/s11104-005-0646-8</pub-id></mixed-citation></ref><ref id="B37"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Kim</surname><given-names>D. Y.</given-names></name><name><surname>Bovet</surname><given-names>L.</given-names></name><name><surname>Maeshima</surname><given-names>M.</given-names></name><name><surname>Martinoia</surname><given-names>E.</given-names></name><name><surname>Lee</surname><given-names>Y.</given-names></name></person-group> (<year>2007</year>). <article-title>The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance</article-title>. <source>Plant J.</source><volume>50</volume>, <fpage>207</fpage>–<lpage>218</lpage>. <pub-id pub-id-type="doi">10.1111/j.1365-313X.2007.03044.x</pub-id><pub-id pub-id-type="pmid">17355438</pub-id></mixed-citation></ref><ref id="B38"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Knight</surname><given-names>H.</given-names></name></person-group> (<year>2000</year>). <article-title>Calcium signaling during abiotic stress in plants</article-title>. <source>Int. Rev. Cytol.</source><volume>195</volume>, <fpage>269</fpage>–<lpage>324</lpage>. <pub-id pub-id-type="doi">10.1016/S0074-7696(08)62707-2</pub-id><pub-id pub-id-type="pmid">10603578</pub-id></mixed-citation></ref><ref id="B39"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>Q.</given-names></name><name><surname>Chen</surname><given-names>L. S.</given-names></name><name><surname>Jiang</surname><given-names>H. X.</given-names></name><name><surname>Tang</surname><given-names>N.</given-names></name><name><surname>Yang</surname><given-names>L. T.</given-names></name><name><surname>Lin</surname><given-names>Z. H.</given-names></name><etal></etal></person-group>. (<year>2010</year>). <article-title>Effects of manganese-excess on CO<sub>2</sub> assimilation, ribulose-1,5-bisphosphate carboxylase/oxygenase, carbohydrates and photosynthetic electron transport of leaves, and antioxidant systems of leaves and roots in <italic>Citrus grandis</italic> seedlings</article-title>. <source>BMC Plant Biol.</source><volume>10</volume>:<fpage>42</fpage>. <pub-id pub-id-type="doi">10.1186/1471-2229-10-42</pub-id><pub-id pub-id-type="pmid">20205939</pub-id></mixed-citation></ref><ref id="B40"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Li</surname><given-names>Y.</given-names></name><name><surname>Han</surname><given-names>M. Q.</given-names></name><name><surname>Lin</surname><given-names>F.</given-names></name><name><surname>Ten</surname><given-names>Y.</given-names></name><name><surname>Lin</surname><given-names>J.</given-names></name><name><surname>Zhu</surname><given-names>D. H.</given-names></name><etal></etal></person-group> (<year>2015</year>). <article-title>Soil chemical properties, ‘Guanximiyou’ pummelo leaf mineral nutrient status and fruit quality in the southern region of Fujian province, China</article-title>. <source>J. Soil Sci. Plant Nutr.</source><volume>15</volume>, <fpage>615</fpage>–<lpage>628</lpage>. <pub-id pub-id-type="doi">10.4067/s0718-95162015005000029</pub-id></mixed-citation></ref><ref id="B41"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Lu</surname><given-names>Y. B.</given-names></name><name><surname>Qi</surname><given-names>Y. P.</given-names></name><name><surname>Lee</surname><given-names>J.</given-names></name><name><surname>Guo</surname><given-names>P.</given-names></name><name><surname>Ye</surname><given-names>X.</given-names></name><name><surname>Jia</surname><given-names>M.</given-names></name><etal></etal></person-group>. (<year>2015</year>). <article-title>Long-term boron-deficiency-responsive genes revealed by cDNA-AFLP differ between <italic>Citrus sinensis</italic> roots and leaves</article-title>. <source>Front. Plant Sci.</source><volume>6</volume>:<issue>585</issue>. <pub-id pub-id-type="doi">10.3389/fpls.2015.00585</pub-id><pub-id pub-id-type="pmid">26284101</pub-id></mixed-citation></ref><ref id="B42"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mao</surname><given-names>C. H.</given-names></name><name><surname>Yi</surname><given-names>K.</given-names></name><name><surname>Yang</surname><given-names>L.</given-names></name><name><surname>Zheng</surname><given-names>B.</given-names></name><name><surname>Wu</surname><given-names>Y.</given-names></name><name><surname>Liu</surname><given-names>F.</given-names></name><etal></etal></person-group> (<year>2004</year>). <article-title>Identification of aluminium regulated genes by cDNA-AFLP in rice (<italic>Oryza sativa</italic> L.): aluminium-regulated genes for the metabolism of cell wall component</article-title>. <source>J. Exp. Bot.</source><volume>55</volume>, <fpage>137</fpage>–<lpage>143</lpage>. <pub-id pub-id-type="doi">10.1093/jxb/erh030</pub-id><pub-id pub-id-type="pmid">14645395</pub-id></mixed-citation></ref><ref id="B43"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Martínez-Cuenca</surname><given-names>M. R.</given-names></name><name><surname>Martínez-Alcántara</surname><given-names>B.</given-names></name><name><surname>Quiñones</surname><given-names>A.</given-names></name><name><surname>Ruiz</surname><given-names>M.</given-names></name><name><surname>Iglesias</surname><given-names>D. J.</given-names></name><name><surname>Primo-Millo</surname><given-names>E.</given-names></name><etal></etal></person-group>. (<year>2015</year>). <article-title>Physiological and molecular responses to excess boron in <italic>Citrus macrophylla</italic> W</article-title>. <source>PLoS ONE</source><volume>10</volume>:<fpage>e0134372</fpage>. <pub-id pub-id-type="doi">10.1371/journal.pone.0134372</pub-id><pub-id pub-id-type="pmid">26225859</pub-id></mixed-citation></ref><ref id="B44"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Maxwell</surname><given-names>D. P.</given-names></name><name><surname>Wang</surname><given-names>Y.</given-names></name><name><surname>McIntosh</surname><given-names>L.</given-names></name></person-group> (<year>1999</year>). <article-title>The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells</article-title>. <source>Proc. Natl. Acad. Sci. U.S.A.</source><volume>96</volume>, <fpage>8271</fpage>–<lpage>8276</lpage>. <pub-id pub-id-type="doi">10.1073/pnas.96.14.8271</pub-id><pub-id pub-id-type="pmid">10393984</pub-id></mixed-citation></ref><ref id="B45"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Mesquita</surname><given-names>G. L.</given-names></name><name><surname>Zambrosi</surname><given-names>F. C. B.</given-names></name><name><surname>Tanaka</surname><given-names>F. A. O.</given-names></name><name><surname>Boaretto</surname><given-names>R. M.</given-names></name><name><surname>Quaggio</surname><given-names>J. A.</given-names></name><name><surname>Ribeiro</surname><given-names>R. V.</given-names></name><etal></etal></person-group>. (<year>2016</year>). <article-title>Anatomical and physiological responses of citrus trees to varying boron availability are dependent on rootstock</article-title>. <source>Front. Plant Sci.</source><volume>7</volume>:<issue>224</issue>. <pub-id pub-id-type="doi">10.3389/fpls.2016.00224</pub-id><pub-id pub-id-type="pmid">26973670</pub-id></mixed-citation></ref><ref id="B46"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Nable</surname><given-names>R. O.</given-names></name><name><surname>Banuelos</surname><given-names>G. S.</given-names></name><name><surname>Paull</surname><given-names>J. G.</given-names></name></person-group> (<year>1997</year>). <article-title>Boron toxicity</article-title>. <source>Plant Soil</source><volume>193</volume>, <fpage>181</fpage>–<lpage>198</lpage>. <pub-id pub-id-type="doi">10.1023/A:1004272227886</pub-id></mixed-citation></ref><ref id="B47"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Padmanabhan</surname><given-names>P.</given-names></name><name><surname>Babaoğlu</surname><given-names>M.</given-names></name><name><surname>Terry</surname><given-names>N.</given-names></name></person-group> (<year>2012</year>). <article-title>A comparative transcriptomic analysis of the extremely boron tolerant plant Puccinellia distans with the moderately boron tolerant <italic>Gypsophila arrostil</italic></article-title>. <source>Plant Cell Rep.</source><volume>31</volume>, <fpage>1407</fpage>–<lpage>1413</lpage>. <pub-id pub-id-type="doi">10.1007/s00299-012-1256-6</pub-id><pub-id pub-id-type="pmid">22484861</pub-id></mixed-citation></ref><ref id="B48"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pandey</surname><given-names>N.</given-names></name><name><surname>Archana</surname></name></person-group> (<year>2013</year>). <article-title>Antioxidant responses and water status in <italic>Brassica</italic> seedlings subjected to boron stress</article-title>. <source>Acta Physiol. Plant.</source><volume>35</volume>, <fpage>697</fpage>–<lpage>706</lpage>. <pub-id pub-id-type="doi">10.1007/s11738-012-1110-z</pub-id></mixed-citation></ref><ref id="B49"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Papadakis</surname><given-names>I. E.</given-names></name><name><surname>Dimassi</surname><given-names>K. N.</given-names></name><name><surname>Bosabalidis</surname><given-names>A. M.</given-names></name><name><surname>Therios</surname><given-names>I. N.</given-names></name><name><surname>Patakas</surname><given-names>A.</given-names></name><name><surname>Giannakoula</surname><given-names>A.</given-names></name></person-group> (<year>2004a</year>). <article-title>Effects of B excess on some physiological and anatomical parameters of ‘Navelina’ orange plants grafted on two rootstocks</article-title>. <source>Environ. Exp. Bot.</source><volume>51</volume>, <fpage>247</fpage>–<lpage>257</lpage>. <pub-id pub-id-type="doi">10.1016/j.envexpbot.2003.11.004</pub-id></mixed-citation></ref><ref id="B50"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Papadakis</surname><given-names>I. E.</given-names></name><name><surname>Dimassi</surname><given-names>K. N.</given-names></name><name><surname>Bosabalidis</surname><given-names>A. M.</given-names></name><name><surname>Therios</surname><given-names>I. N.</given-names></name><name><surname>Patakas</surname><given-names>A.</given-names></name><name><surname>Giannakoula</surname><given-names>A.</given-names></name></person-group> (<year>2004b</year>). <article-title>Boron toxicity in ‘Clementine’ mandarin plants grafted on two rootstocks</article-title>. <source>Plant Sci.</source><volume>166</volume>, <fpage>539</fpage>–<lpage>547</lpage>. <pub-id pub-id-type="doi">10.1016/j.plantsci.2003.10.027</pub-id></mixed-citation></ref><ref id="B51"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Papadakis</surname><given-names>I. E.</given-names></name><name><surname>Dimassi</surname><given-names>K. N.</given-names></name><name><surname>Therios</surname><given-names>I. N.</given-names></name></person-group> (<year>2003</year>). <article-title>Response of two citrus genotypes to six boron concentrations: concentration and distribution of nutrients, total absorption, and nutrient use efficiency</article-title>. <source>Aust. J. Agr. Res.</source><volume>54</volume>, <fpage>571</fpage>–<lpage>580</lpage>. <pub-id pub-id-type="doi">10.1071/AR02163</pub-id></mixed-citation></ref><ref id="B52"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Paun</surname><given-names>O.</given-names></name><name><surname>Schönswetter</surname><given-names>P.</given-names></name></person-group> (<year>2012</year>). <article-title>Amplified fragment length polymorphism: an invaluable fingerprinting technique for genomic, transcriptomic, and epigenetic studies</article-title>. <source>Methods Mol. Biol.</source><volume>862</volume>, <fpage>75</fpage>–<lpage>87</lpage>. <pub-id pub-id-type="doi">10.1007/978-1-61779-609-8_7</pub-id><pub-id pub-id-type="pmid">22419490</pub-id></mixed-citation></ref><ref id="B53"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Pérez-Gilabert</surname><given-names>M.</given-names></name><name><surname>López-Nicolás</surname><given-names>J. M.</given-names></name><name><surname>Carmona</surname><given-names>F. G.</given-names></name></person-group> (<year>2001</year>). <article-title>Purification of a novel lipoxygenase from eggplant (<italic>Solanum melongena</italic>) fruit chloroplast</article-title>. <source>Physiol. Plant.</source><volume>111</volume>, <fpage>276</fpage>–<lpage>282</lpage>. <pub-id pub-id-type="doi">10.1034/j.1399-3054.2001.1110303.x</pub-id><pub-id pub-id-type="pmid">11240910</pub-id></mixed-citation></ref><ref id="B54"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Popov</surname><given-names>V. N.</given-names></name><name><surname>Simonian</surname><given-names>R. A.</given-names></name><name><surname>Skulachev</surname><given-names>V. P.</given-names></name><name><surname>Starkov</surname><given-names>A. A.</given-names></name></person-group> (<year>1997</year>). <article-title>Inhibition of the alternative oxidase stimulates H<sub>2</sub>O<sub>2</sub> production in plant mitochondria</article-title>. <source>FEBS Lett.</source><volume>415</volume>, <fpage>87</fpage>–<lpage>90</lpage>. <pub-id pub-id-type="doi">10.1016/S0014-5793(97)01099-5</pub-id><pub-id pub-id-type="pmid">9326375</pub-id></mixed-citation></ref><ref id="B55"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Purvis</surname><given-names>A. C.</given-names></name></person-group> (<year>1997</year>). <article-title>Role of the alternative oxidase in limiting superoxide production in plant mitochondria</article-title>. <source>Physiol. Plant.</source><volume>100</volume>, <fpage>165</fpage>–<lpage>170</lpage>. <pub-id pub-id-type="doi">10.1111/j.1399-3054.1997.tb03468.x</pub-id></mixed-citation></ref><ref id="B56"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Reid</surname><given-names>R. J.</given-names></name><name><surname>Hayes</surname><given-names>J. E.</given-names></name><name><surname>Post</surname><given-names>A.</given-names></name><name><surname>Stangoulis</surname><given-names>J. C. R.</given-names></name><name><surname>Graham</surname><given-names>R. D.</given-names></name></person-group> (<year>2004</year>). <article-title>A critical analysis of the causes of boron toxicity in plants</article-title>. <source>Plant Cell Environ.</source><volume>27</volume>, <fpage>1405</fpage>–<lpage>1414</lpage>. <pub-id pub-id-type="doi">10.1111/j.1365-3040.2004.01243.x</pub-id></mixed-citation></ref><ref id="B57"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sakamoto</surname><given-names>T.</given-names></name><name><surname>Inui</surname><given-names>Y. T.</given-names></name><name><surname>Uraguchi</surname><given-names>S.</given-names></name><name><surname>Yoshizumi</surname><given-names>T.</given-names></name><name><surname>Matsunaga</surname><given-names>S.</given-names></name><name><surname>Mastui</surname><given-names>M.</given-names></name><etal></etal></person-group>. (<year>2011</year>). <article-title>Condensin II alleviates DNA damage and is essential for tolerance of boron overload stress in <italic>Arabidopsis</italic></article-title>. <source>Plant Cell</source><volume>23</volume>, <fpage>3533</fpage>–<lpage>3546</lpage>. <pub-id pub-id-type="doi">10.1105/tpc.111.086314</pub-id><pub-id pub-id-type="pmid">21917552</pub-id></mixed-citation></ref><ref id="B58"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sang</surname><given-names>W.</given-names></name><name><surname>Huang</surname><given-names>Z. R.</given-names></name><name><surname>Qi</surname><given-names>Y. P.</given-names></name><name><surname>Yang</surname><given-names>L. T.</given-names></name><name><surname>Guo</surname><given-names>P.</given-names></name><name><surname>Chen</surname><given-names>L. S.</given-names></name></person-group> (<year>2015</year>). <article-title>An investigation of boron-toxicity in leaves of two citrus species differing in boron-tolerance using comparative proteomics</article-title>. <source>J. Proteomics</source><volume>123</volume>, <fpage>128</fpage>–<lpage>146</lpage>. <pub-id pub-id-type="doi">10.1016/j.jprot.2015.04.007</pub-id><pub-id pub-id-type="pmid">25892131</pub-id></mixed-citation></ref><ref id="B59"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Schnurbusch</surname><given-names>T.</given-names></name><name><surname>Hayes</surname><given-names>J.</given-names></name><name><surname>Hrmova</surname><given-names>M.</given-names></name><name><surname>Baumann</surname><given-names>U.</given-names></name><name><surname>Ramesh</surname><given-names>S. A.</given-names></name><name><surname>Tyerman</surname><given-names>S. D.</given-names></name><etal></etal></person-group>. (<year>2010</year>). <article-title>Boron toxicity tolerance in barley through reduced expression of the multifunctional aquaporin HvNIP2;1</article-title>. <source>Plant Physiol.</source><volume>153</volume>, <fpage>1706</fpage>–<lpage>1715</lpage>. <pub-id pub-id-type="doi">10.1104/pp.110.158832</pub-id><pub-id pub-id-type="pmid">20581256</pub-id></mixed-citation></ref><ref id="B60"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Sheng</surname><given-names>O.</given-names></name><name><surname>Zhou</surname><given-names>G. F.</given-names></name><name><surname>Wei</surname><given-names>Q. J.</given-names></name><name><surname>Peng</surname><given-names>S. A.</given-names></name><name><surname>Deng</surname><given-names>X. X.</given-names></name></person-group> (<year>2010</year>). <article-title>Effects of excess boron on growth, gas exchange, and boron status of four orange scion-rootstock combinations</article-title>. <source>J. Plant. Nutr. Soil Sci.</source><volume>173</volume>, <fpage>469</fpage>–<lpage>476</lpage>. <pub-id pub-id-type="doi">10.1002/jpln.200800273</pub-id></mixed-citation></ref><ref id="B61"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Shockey</surname><given-names>J.</given-names></name><name><surname>Regmi</surname><given-names>A.</given-names></name><name><surname>Cotton</surname><given-names>K.</given-names></name><name><surname>Adhikari</surname><given-names>N.</given-names></name><name><surname>Browse</surname><given-names>J.</given-names></name><name><surname>Bates</surname><given-names>P. D.</given-names></name></person-group> (<year>2016</year>). <article-title>Identification of <italic>Arabidopsis GPAT9</italic> (At5g60620) as an essential gene involved in triacylglycerol biosynthesis</article-title>. <source>Plant Physiol.</source><volume>170</volume>, <fpage>163</fpage>–<lpage>179</lpage>. <pub-id pub-id-type="doi">10.1104/pp.15.01563</pub-id><pub-id pub-id-type="pmid">26586834</pub-id></mixed-citation></ref><ref id="B62"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Siddiqui</surname><given-names>M. H.</given-names></name><name><surname>Al-Whaibi</surname><given-names>M. H.</given-names></name><name><surname>Sakran</surname><given-names>A. M.</given-names></name><name><surname>Ali</surname><given-names>H. M.</given-names></name><name><surname>Basalah</surname><given-names>M. O.</given-names></name><name><surname>Faisal</surname><given-names>M.</given-names></name><etal></etal></person-group> (<year>2013</year>). <article-title>Calcium-induced amelioration of boron toxicity in radish</article-title>. <source>J. Plant Growth Regul.</source><volume>32</volume>, <fpage>61</fpage>–<lpage>71</lpage>. <pub-id pub-id-type="doi">10.1007/s00344-012-9276-6</pub-id></mixed-citation></ref><ref id="B63"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Singh</surname><given-names>J. P.</given-names></name><name><surname>Dahiya</surname><given-names>D. J.</given-names></name><name><surname>Narwal</surname><given-names>R. P.</given-names></name></person-group> (<year>1990</year>). <article-title>Boron uptake and toxicity in wheat in relation to zinc supply</article-title>. <source>Fert. Res.</source><volume>24</volume>, <fpage>105</fpage>–<lpage>110</lpage>. <pub-id pub-id-type="doi">10.1007/BF01073228</pub-id></mixed-citation></ref><ref id="B64"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tian</surname><given-names>L.</given-names></name><name><surname>Chen</surname><given-names>Z. J.</given-names></name></person-group> (<year>2001</year>). <article-title>Blocking histone deacetylation in <italic>Arabidopsis</italic> induces pleiotropic effects on plant gene regulation and development</article-title>. <source>Proc. Natl. Acad. Sci. U.S.A.</source><volume>98</volume>, <fpage>200</fpage>–<lpage>205</lpage>. <pub-id pub-id-type="doi">10.1073/pnas.98.1.200</pub-id><pub-id pub-id-type="pmid">11134508</pub-id></mixed-citation></ref><ref id="B65"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tian</surname><given-names>L.</given-names></name><name><surname>Wang</surname><given-names>J.</given-names></name><name><surname>Fong</surname><given-names>M. P.</given-names></name><name><surname>Chen</surname><given-names>M.</given-names></name><name><surname>Cao</surname><given-names>H.</given-names></name><name><surname>Gelvin</surname><given-names>S. B.</given-names></name><etal></etal></person-group>. (<year>2003</year>). <article-title>Genetic control of developmental changes induced by disruption of <italic>Arabidopsis histone deacetylase 1</italic> (<italic>AtHD1</italic>) expression</article-title>. <source>Genetics</source><volume>165</volume>, <fpage>399</fpage>–<lpage>409</lpage>. <pub-id pub-id-type="pmid">14504245</pub-id></mixed-citation></ref><ref id="B66"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tirajoh</surname><given-names>A.</given-names></name><name><surname>Aung</surname><given-names>T. S.</given-names></name><name><surname>McKay</surname><given-names>A. B.</given-names></name><name><surname>Plant</surname><given-names>A. L.</given-names></name></person-group> (<year>2005</year>). <article-title>Stress-responsive α<italic>-dioxygenase</italic> expression in tomato roots</article-title>. <source>J. Exp. Bot.</source><volume>56</volume>, <fpage>713</fpage>–<lpage>723</lpage>. <pub-id pub-id-type="doi">10.1093/jxb/eri038</pub-id><pub-id pub-id-type="pmid">15618300</pub-id></mixed-citation></ref><ref id="B67"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Tombuloglu</surname><given-names>G.</given-names></name><name><surname>Tombuloglu</surname><given-names>H.</given-names></name><name><surname>Sakcali</surname><given-names>M. S.</given-names></name><name><surname>Unver</surname><given-names>T.</given-names></name></person-group> (<year>2015</year>). <article-title>High-throughput transcriptome analysis of barley (<italic>Hordeum vulgare</italic>) exposed to excessive boron</article-title>. <source>Gene</source><volume>557</volume>, <fpage>71</fpage>–<lpage>81</lpage>. <pub-id pub-id-type="doi">10.1016/j.gene.2014.12.012</pub-id><pub-id pub-id-type="pmid">25498907</pub-id></mixed-citation></ref><ref id="B68"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Turan</surname><given-names>M. A.</given-names></name><name><surname>Taban</surname><given-names>N.</given-names></name><name><surname>Taban</surname><given-names>S.</given-names></name></person-group> (<year>2009</year>). <article-title>Effect of calcium on the alleviation of boron toxicity and localization of boron and calcium in cell wall of wheat</article-title>. <source>Not. Bot. Hort. Agrobot. Cluj.</source><volume>37</volume>, <fpage>99</fpage>–<lpage>103</lpage>.</mixed-citation></ref><ref id="B69"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Turner</surname><given-names>B. M.</given-names></name></person-group> (<year>2002</year>). <article-title>Cellular memory and the histone code</article-title>. <source>Cell</source><volume>111</volume>, <fpage>285</fpage>–<lpage>291</lpage>. <pub-id pub-id-type="doi">10.1016/S0092-8674(02)01080-2</pub-id><pub-id pub-id-type="pmid">12419240</pub-id></mixed-citation></ref><ref id="B70"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Vantini</surname><given-names>J. S.</given-names></name><name><surname>Dedemo</surname><given-names>G. C.</given-names></name><name><surname>Jovino Gimenez</surname><given-names>D. F.</given-names></name><name><surname>Fonseca</surname><given-names>L. F.</given-names></name><name><surname>Tezza</surname><given-names>R. I.</given-names></name><name><surname>Mutton</surname><given-names>M. A.</given-names></name><etal></etal></person-group>. (<year>2015</year>). <article-title>Differential gene expression in drought-tolerant sugarcane roots</article-title>. <source>Genet. Mol. Res.</source><volume>14</volume>, <fpage>7196</fpage>–<lpage>7207</lpage>. <pub-id pub-id-type="doi">10.4238/2015.June.29.13</pub-id><pub-id pub-id-type="pmid">26125930</pub-id></mixed-citation></ref><ref id="B71"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>C.</given-names></name><name><surname>Liu</surname><given-names>Z.</given-names></name></person-group> (<year>2006</year>). <article-title><italic>Arabidopsis</italic> ribonucleotide reductases are critical for cell cycle progression, DNA damage repair, and plant development</article-title>. <source>Plant Cell</source><volume>18</volume>, <fpage>350</fpage>–<lpage>365</lpage>. <pub-id pub-id-type="doi">10.1105/tpc.105.037044</pub-id><pub-id pub-id-type="pmid">16399800</pub-id></mixed-citation></ref><ref id="B72"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>H. Y.</given-names></name><name><surname>Lee</surname><given-names>M. M.</given-names></name><name><surname>Schiefelbein</surname><given-names>J. W.</given-names></name></person-group> (<year>2002</year>). <article-title>Regulation of the cell expansion gene <italic>RHD3</italic> during <italic>Arabidopsis</italic> development</article-title>. <source>Plant Physiol.</source><volume>129</volume>, <fpage>638</fpage>–<lpage>649</lpage>. <pub-id pub-id-type="doi">10.1104/pp.002675</pub-id><pub-id pub-id-type="pmid">12068108</pub-id></mixed-citation></ref><ref id="B73"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>J.</given-names></name><name><surname>Nakazato</surname><given-names>T.</given-names></name><name><surname>Sakanishi</surname><given-names>K.</given-names></name><name><surname>Yamada</surname><given-names>O.</given-names></name><name><surname>Tao</surname><given-names>H.</given-names></name><name><surname>Saito</surname><given-names>I.</given-names></name></person-group> (<year>2006</year>). <article-title>Single-step microwave digestion with HNO<sub>3</sub> alone for determination of trace elements in coal by ICP spectrometry</article-title>. <source>Talanta</source><volume>68</volume>, <fpage>1584</fpage>–<lpage>1590</lpage>. <pub-id pub-id-type="doi">10.1016/j.talanta.2005.08.034</pub-id><pub-id pub-id-type="pmid">18970502</pub-id></mixed-citation></ref><ref id="B74"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname><given-names>Z. Y.</given-names></name><name><surname>Shen</surname><given-names>K.</given-names></name><name><surname>Zhang</surname><given-names>F. S.</given-names></name></person-group> (<year>1995</year>). <article-title>Effect of boron on nucleic acid metabolism in <italic>Brassica napus</italic> L</article-title>. <source>Acta Phytophysiol. Sin.</source><volume>21</volume>, <fpage>189</fpage>–<lpage>194</lpage>.</mixed-citation></ref><ref id="B75"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Xu</surname><given-names>M.</given-names></name><name><surname>Xie</surname><given-names>W.</given-names></name><name><surname>Huang</surname><given-names>M.</given-names></name></person-group> (<year>2012</year>). <article-title>Overexpression of <italic>PeRHD3</italic> alters the root architecture in <italic>Populus</italic></article-title>. <source>Biochem. Biophys. Res. Commun.</source><volume>424</volume>, <fpage>239</fpage>–<lpage>244</lpage>. <pub-id pub-id-type="doi">10.1016/j.bbrc.2012.06.083</pub-id><pub-id pub-id-type="pmid">22732403</pub-id></mixed-citation></ref><ref id="B76"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Xu</surname><given-names>X. M.</given-names></name><name><surname>Lin</surname><given-names>H.</given-names></name><name><surname>Maple</surname><given-names>J.</given-names></name><name><surname>Björkblom</surname><given-names>B.</given-names></name><name><surname>Alves</surname><given-names>G.</given-names></name><name><surname>Larsen</surname><given-names>J. P.</given-names></name><etal></etal></person-group>. (<year>2010</year>). <article-title>The <italic>Arabidopsis</italic> DJ-1a protein confers stress protection through cytosolic SOD activation</article-title>. <source>J. Cell Sci.</source><volume>123</volume>, <fpage>1644</fpage>–<lpage>1651</lpage>. <pub-id pub-id-type="doi">10.1242/jcs.063222</pub-id><pub-id pub-id-type="pmid">20406884</pub-id></mixed-citation></ref><ref id="B77"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Xu</surname><given-names>X. M.</given-names></name><name><surname>Møller</surname><given-names>S. G.</given-names></name></person-group> (<year>2010</year>). <article-title>ROS removal by DJ-1: <italic>Arabidopsis</italic> as a new model to understand Parkinson's disease</article-title>. <source>Plant Signal. Behav.</source><volume>5</volume>, <fpage>1034</fpage>–<lpage>1046</lpage>. <pub-id pub-id-type="doi">10.4161/psb.5.8.12298</pub-id><pub-id pub-id-type="pmid">20671441</pub-id></mixed-citation></ref><ref id="B78"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhang</surname><given-names>Y.</given-names></name><name><surname>Xiao</surname><given-names>Y.</given-names></name><name><surname>Du</surname><given-names>F.</given-names></name><name><surname>Cao</surname><given-names>L.</given-names></name><name><surname>Dong</surname><given-names>H.</given-names></name><name><surname>Ren</surname><given-names>H.</given-names></name></person-group> (<year>2011</year>). <article-title><italic>Arabidopsis</italic> VILLIN4 is involved in root hair growth through regulating actin organization in a Ca<sup>2+</sup>-dependent manner</article-title>. <source>New Phytol.</source><volume>190</volume>, <fpage>667</fpage>–<lpage>682</lpage>. <pub-id pub-id-type="doi">10.1111/j.1469-8137.2010.03632.x</pub-id><pub-id pub-id-type="pmid">21275995</pub-id></mixed-citation></ref><ref id="B79"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhou</surname><given-names>C. P.</given-names></name><name><surname>Qi</surname><given-names>Y. P.</given-names></name><name><surname>You</surname><given-names>X.</given-names></name><name><surname>Yang</surname><given-names>L. T.</given-names></name><name><surname>Guo</surname><given-names>P.</given-names></name><name><surname>Ye</surname><given-names>X.</given-names></name><etal></etal></person-group>. (<year>2013</year>). <article-title>Leaf cDNA-AFLP analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity</article-title>. <source>BMC Genomics</source><volume>14</volume>:<fpage>621</fpage>. <pub-id pub-id-type="doi">10.1186/1471-2164-14-621</pub-id><pub-id pub-id-type="pmid">24034812</pub-id></mixed-citation></ref><ref id="B80"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zhu</surname><given-names>Y.</given-names></name><name><surname>Dong</surname><given-names>A.</given-names></name><name><surname>Shen</surname><given-names>W. H.</given-names></name></person-group> (<year>2012</year>). <article-title>Histone variants and chromatin assembly in plant abiotic stress responses</article-title>. <source>Biochim. Biophys. Acta</source><volume>1819</volume>, <fpage>343</fpage>–<lpage>348</lpage>. <pub-id pub-id-type="doi">10.1016/j.bbagrm.2011.07.012</pub-id><pub-id pub-id-type="pmid">24459736</pub-id></mixed-citation></ref><ref id="B81"><mixed-citation publication-type="journal"><person-group person-group-type="author"><name><surname>Zidenga</surname><given-names>T.</given-names></name><name><surname>Leyva-Guerrero</surname><given-names>E.</given-names></name><name><surname>Moon</surname><given-names>H.</given-names></name><name><surname>Siritunga</surname><given-names>D.</given-names></name><name><surname>Sayre</surname><given-names>R.</given-names></name></person-group> (<year>2012</year>). <article-title>Extending cassava root shelf life <italic>via</italic> reduction of reactive oxygen species production</article-title>. <source>Plant Physiol.</source><volume>159</volume>, <fpage>1396</fpage>–<lpage>1407</lpage>. <pub-id pub-id-type="doi">10.1104/pp.112.200345</pub-id><pub-id pub-id-type="pmid">22711743</pub-id></mixed-citation></ref></ref-list></back></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 0003280 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 0003280 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.25. |  |