Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C</title><author><name sortKey="Jiang, Shi Ming" sort="Jiang, Shi Ming" uniqKey="Jiang S" first="Shi-Ming" last="Jiang">Shi-Ming Jiang</name></author><author><name sortKey="Xu, Zhao Hui" sort="Xu, Zhao Hui" uniqKey="Xu Z" first="Zhao-Hui" last="Xu">Zhao-Hui Xu</name></author><author><name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name></author><author><name sortKey="Shi, Xian Min" sort="Shi, Xian Min" uniqKey="Shi X" first="Xian-Min" last="Shi">Xian-Min Shi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27239129</idno><idno type="pmc">4842870</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842870</idno><idno type="RBID">PMC:4842870</idno><idno type="doi">10.3748/wjg.v3.i3.150</idno><date when="1997">1997</date><idno type="wicri:Area/Pmc/Corpus">000274</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C</title><author><name sortKey="Jiang, Shi Ming" sort="Jiang, Shi Ming" uniqKey="Jiang S" first="Shi-Ming" last="Jiang">Shi-Ming Jiang</name></author><author><name sortKey="Xu, Zhao Hui" sort="Xu, Zhao Hui" uniqKey="Xu Z" first="Zhao-Hui" last="Xu">Zhao-Hui Xu</name></author><author><name sortKey="Zhang, Yan" sort="Zhang, Yan" uniqKey="Zhang Y" first="Yan" last="Zhang">Yan Zhang</name></author><author><name sortKey="Shi, Xian Min" sort="Shi, Xian Min" uniqKey="Shi X" first="Xian-Min" last="Shi">Xian-Min Shi</name></author></analytic><series><title level="j">World Journal of Gastroenterology</title><idno type="ISSN">1007-9327</idno><idno type="eISSN">2219-2840</idno><imprint><date when="1997">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.</p><p>METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.</p><p>RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.</p><p>CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">World J Gastroenterol</journal-id><journal-id journal-id-type="iso-abbrev">World J. Gastroenterol</journal-id><journal-id journal-id-type="publisher-id">WJG</journal-id><journal-title-group><journal-title>World Journal of Gastroenterology</journal-title></journal-title-group><issn pub-type="ppub">1007-9327</issn><issn pub-type="epub">2219-2840</issn><publisher><publisher-name>Baishideng Publishing Group Inc</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27239129</article-id><article-id pub-id-type="pmc">4842870</article-id><article-id pub-id-type="other">jWJG.v3.i3.pg150</article-id><article-id pub-id-type="doi">10.3748/wjg.v3.i3.150</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Research</subject></subj-group></article-categories><title-group><article-title>Survival and malignant phenotype changes of human hepatoma SMMC-7721 cell line induced by cryopreservation at -50 °C</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Shi-Ming</given-names></name></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Zhao-Hui</given-names></name></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Yan</given-names></name></contrib><contrib contrib-type="author"><name><surname>Shi</surname><given-names>Xian-Min</given-names></name></contrib><aff>Shi-Ming Jiang, Zhao-Hui Xu, Biology Department, Shandong Normal University, Jinan 250014, Shandong Province, China</aff><aff>Yan Zhang, Shanghai Institute of Cell Biology, Academia Sinica</aff><aff>Xian-Min Shi, Biology Department, Xiamen University</aff></contrib-group><author-notes><fn><p>Author contributions: All authors contributed equally to the work.</p><p>Correspondence to: Dr. Shi-Ming Jiang, Biology Department, Shandong Normal University, Jinan 250014, Shandong Province, China</p></fn></author-notes><pub-date pub-type="ppub"><day>15</day><month>9</month><year>1997</year></pub-date><pub-date pub-type="epub"><day>15</day><month>9</month><year>1997</year></pub-date><volume>3</volume><issue>3</issue><fpage>150</fpage><lpage>152</lpage><history><date date-type="received"><day>26</day><month>10</month><year>1996</year></date><date date-type="rev-recd"><day>19</day><month>12</month><year>1996</year></date><date date-type="accepted"><day>22</day><month>1</month><year>1997</year></date></history><permissions><copyright-statement>©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.</copyright-statement><copyright-year>1997</copyright-year></permissions><abstract><p>AIM: To investigate the effect of cryopreservation at -50 °C on the human hepatoma SMMC-7721 cell line.</p><p>METHODS: With 15% DMSO as a cryoprotectant, the SMMC-7721 cells were cryopreserved at -50 °C, then thawed and recultured. The survival rate, mitotic index and LDH isoenzymes were compared between pre- and post-cryopreservation.</p><p>RESULTS: Thirteen hours after the thaw, the mitotic index of cryopreserved SMMC-7721 cells decreased by 1.09%. The mode scope of chromosome number (46-53) after cryopreservation tended to transfer to that of normal human cells, and the percentage of metaphases containing 46 chromosomes changed from 0% to 16%. LDH isoenzymes changed from H-like model (LDH3(29.3%) > LDH4 (26.8%) > LDH2 (25.3%) > LDH5 (14.9%) > LDH1 (3.6%) to M-like model (LDH4 (48.3%) > LDH5 (28.3%) > LDH3 (18.9%) > LDH2 (4.4%) > LDH1 (0%)). This suggests that the survival rate could reach over 95%.</p><p>CONCLUSION: Cryopreservation at -50 °C can be a convenient method for the cryopreservation of cell lines. However, cryopreservation at -50 °C is likely involved in the changes of the malignant phenotypes of the human hepatoma SMMC-7721 cell line, and may induce the differentiation of malignant cells.</p></abstract><kwd-group><kwd>Liver neoplasms</kwd><kwd>Carcinoma, hepatocellular</kwd><kwd>Cryopreservation</kwd><kwd>Tumor cells, cultured</kwd><kwd>Phenotype</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000274 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000274 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.25. |  |