Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis
Identifieur interne : 001224 ( Pmc/Checkpoint ); précédent : 001223; suivant : 001225Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis
Auteurs : Helvécio Della Coletta-Filho ; Marco Aurélio Takita ; Alessandra Alves De Souza ; Carlos Ivan Aguilar-Vildoso ; Marcos Antonio MachadoSource :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2001.
Abstract
Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen
Url:
DOI: 10.1128/AEM.67.9.4091-4095.2001
PubMed: 11526010
PubMed Central: 93134
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differentiation of Strains of <italic>Xylella fastidiosa</italic>
by a Variable Number of Tandem Repeat Analysis</title>
<author><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
</author>
<author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</author>
<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
</author>
<author><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Differentiation of Strains of <italic>Xylella fastidiosa</italic>
by a Variable Number of Tandem Repeat Analysis</title>
<author><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
</author>
<author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</author>
<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
</author>
<author><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
</author>
</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2001">2001</date>
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<front><div type="abstract" xml:lang="en"><p>Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen <italic>Xylella fastidiosa</italic>
and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 <italic>X. fastidiosa</italic>
strains from different hosts. The highest average Nei's measure of genetic diversity (<italic>H</italic>
) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus <italic>X. fastidiosa</italic>
strains, some specific VNTR loci had a <italic>H</italic>
value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific <italic>X. fastidiosa</italic>
strains.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">APPL ENVIRON MICROBIOL</journal-id>
<journal-title>Applied and Environmental Microbiology</journal-title>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">11526010</article-id>
<article-id pub-id-type="pmc">93134</article-id>
<article-id pub-id-type="publisher-id">0489</article-id>
<article-id pub-id-type="doi">10.1128/AEM.67.9.4091-4095.2001</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Plant Microbiology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Differentiation of Strains of <italic>Xylella fastidiosa</italic>
by a Variable Number of Tandem Repeat Analysis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Coletta-Filho</surname>
<given-names>Helvécio Della</given-names>
</name>
<xref ref-type="author-notes" rid="FN150">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Takita</surname>
<given-names>Marco Aurélio</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>de Souza</surname>
<given-names>Alessandra Alves</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Aguilar-Vildoso</surname>
<given-names>Carlos Ivan</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Machado</surname>
<given-names>Marcos Antonio</given-names>
</name>
</contrib>
</contrib-group>
<aff id="N0x96e9d20.0x9673a28">Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, Cordeirópolis, São Paulo, Brazil</aff>
<author-notes><fn id="FN150"><label>*</label>
<p>Corresponding author. Mailing address: Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, CP04, Cordeirópolis, São Paulo, Brazil. Phone and fax: 19 546-1399. E-mail: <email>helvecio@centrodecitricultura.br</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>9</month>
<year>2001</year>
</pub-date>
<volume>67</volume>
<issue>9</issue>
<fpage>4091</fpage>
<lpage>4095</lpage>
<history><date date-type="received"><day>27</day>
<month>3</month>
<year>2001</year>
</date>
<date date-type="accepted"><day>27</day>
<month>6</month>
<year>2001</year>
</date>
</history>
<copyright-statement>Copyright © 2001, American Society for Microbiology</copyright-statement>
<copyright-year>2001</copyright-year>
<abstract><p>Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen <italic>Xylella fastidiosa</italic>
and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 <italic>X. fastidiosa</italic>
strains from different hosts. The highest average Nei's measure of genetic diversity (<italic>H</italic>
) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus <italic>X. fastidiosa</italic>
strains, some specific VNTR loci had a <italic>H</italic>
value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific <italic>X. fastidiosa</italic>
strains.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
<name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
<name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
<name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
<name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</noCountry>
</tree>
</affiliations>
</record>
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