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Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Identifieur interne : 001224 ( Pmc/Checkpoint ); précédent : 001223; suivant : 001225

Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Auteurs : Helvécio Della Coletta-Filho ; Marco Aurélio Takita ; Alessandra Alves De Souza ; Carlos Ivan Aguilar-Vildoso ; Marcos Antonio Machado

Source :

Applied and Environmental Microbiology [ 0099-2240 ] ; 2001.

RBID : PMC:93134

Abstract

Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen Xylella fastidiosa and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 X. fastidiosa strains from different hosts. The highest average Nei's measure of genetic diversity (H) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus X. fastidiosa strains, some specific VNTR loci had a H value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific X. fastidiosa strains.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93134

DOI: 10.1128/AEM.67.9.4091-4095.2001
PubMed: 11526010
PubMed Central: 93134

Affiliations:

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PMC:93134

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differentiation of Strains of <italic>Xylella fastidiosa</italic>
 by a Variable Number of Tandem Repeat Analysis</title>
<author><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
</author>
<author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</author>
<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
</author>
<author><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Differentiation of Strains of <italic>Xylella fastidiosa</italic>
 by a Variable Number of Tandem Repeat Analysis</title>
<author><name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
</author>
<author><name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</author>
<author><name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
</author>
<author><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
</author>
</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2001">2001</date>
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<profileDesc><textClass></textClass>
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<front><div type="abstract" xml:lang="en"><p>Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen <italic>Xylella fastidiosa</italic>
 and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 <italic>X. fastidiosa</italic>
 strains from different hosts. The highest average Nei's measure of genetic diversity (<italic>H</italic>
) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus <italic>X. fastidiosa</italic>
 strains, some specific VNTR loci had a <italic>H</italic>
 value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific <italic>X. fastidiosa</italic>
 strains.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id>
<journal-id journal-id-type="publisher-id">APPL ENVIRON MICROBIOL</journal-id>
<journal-title>Applied and Environmental Microbiology</journal-title>
<issn pub-type="ppub">0099-2240</issn>
<issn pub-type="epub">1098-5336</issn>
<publisher><publisher-name>American Society for Microbiology</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">11526010</article-id>
<article-id pub-id-type="pmc">93134</article-id>
<article-id pub-id-type="publisher-id">0489</article-id>
<article-id pub-id-type="doi">10.1128/AEM.67.9.4091-4095.2001</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Plant Microbiology</subject>
</subj-group>
</article-categories>
<title-group><article-title>Differentiation of Strains of <italic>Xylella fastidiosa</italic>
 by a Variable Number of Tandem Repeat Analysis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Coletta-Filho</surname>
<given-names>Helvécio Della</given-names>
</name>
<xref ref-type="author-notes" rid="FN150">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Takita</surname>
<given-names>Marco Aurélio</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>de Souza</surname>
<given-names>Alessandra Alves</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Aguilar-Vildoso</surname>
<given-names>Carlos Ivan</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Machado</surname>
<given-names>Marcos Antonio</given-names>
</name>
</contrib>
</contrib-group>
<aff id="N0x96e9d20.0x9673a28">Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, Cordeirópolis, São Paulo, Brazil</aff>
<author-notes><fn id="FN150"><label>*</label>
<p>Corresponding author. Mailing address: Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, CP04, Cordeirópolis, São Paulo, Brazil. Phone and fax: 19 546-1399. E-mail: <email>helvecio@centrodecitricultura.br</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>9</month>
<year>2001</year>
</pub-date>
<volume>67</volume>
<issue>9</issue>
<fpage>4091</fpage>
<lpage>4095</lpage>
<history><date date-type="received"><day>27</day>
<month>3</month>
<year>2001</year>
</date>
<date date-type="accepted"><day>27</day>
<month>6</month>
<year>2001</year>
</date>
</history>
<copyright-statement>Copyright © 2001, American Society for Microbiology</copyright-statement>
<copyright-year>2001</copyright-year>
<abstract><p>Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen <italic>Xylella fastidiosa</italic>
 and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27 <italic>X. fastidiosa</italic>
 strains from different hosts. The highest average Nei's measure of genetic diversity (<italic>H</italic>
) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus <italic>X. fastidiosa</italic>
 strains, some specific VNTR loci had a <italic>H</italic>
 value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific <italic>X. fastidiosa</italic>
 strains.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Aguilar Vildoso, Carlos Ivan" sort="Aguilar Vildoso, Carlos Ivan" uniqKey="Aguilar Vildoso C" first="Carlos Ivan" last="Aguilar-Vildoso">Carlos Ivan Aguilar-Vildoso</name>
<name sortKey="Coletta Filho, Helvecio Della" sort="Coletta Filho, Helvecio Della" uniqKey="Coletta Filho H" first="Helvécio Della" last="Coletta-Filho">Helvécio Della Coletta-Filho</name>
<name sortKey="De Souza, Alessandra Alves" sort="De Souza, Alessandra Alves" uniqKey="De Souza A" first="Alessandra Alves" last="De Souza">Alessandra Alves De Souza</name>
<name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
<name sortKey="Takita, Marco Aurelio" sort="Takita, Marco Aurelio" uniqKey="Takita M" first="Marco Aurélio" last="Takita">Marco Aurélio Takita</name>
</noCountry>
</tree>
</affiliations>
</record>

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Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

Differentiation of Strains of Xylella fastidiosa by a Variable Number of Tandem Repeat Analysis

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Abstract

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