Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis ZINC FINGER PROTEIN21[C][W][OA]
Identifieur interne : 001058 ( Pmc/Checkpoint ); précédent : 001057; suivant : 001059Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis ZINC FINGER PROTEIN21[C][W][OA]
Auteurs : Suqin Cai ; Coralie C. LashbrookSource :
- Plant Physiology [ 0032-0889 ] ; 2008.
Abstract
Organ detachment requires cell separation within abscission zones (AZs). Physiological studies have established that ethylene and auxin contribute to cell separation control. Genetic analyses of abscission mutants have defined ethylene-independent detachment regulators. Functional genomic strategies leading to global understandings of abscission have awaited methods for isolating AZ cells of low abundance and very small size. Here, we couple laser capture microdissection of
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DOI: 10.1104/pp.107.110908
PubMed: 18192438
PubMed Central: 2259061
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<author><name sortKey="Lashbrook, Coralie C" sort="Lashbrook, Coralie C" uniqKey="Lashbrook C" first="Coralie C." last="Lashbrook">Coralie C. Lashbrook</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis <italic>ZINC FINGER PROTEIN2</italic>
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<author><name sortKey="Lashbrook, Coralie C" sort="Lashbrook, Coralie C" uniqKey="Lashbrook C" first="Coralie C." last="Lashbrook">Coralie C. Lashbrook</name>
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<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
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<front><div type="abstract" xml:lang="en"><p>Organ detachment requires cell separation within abscission zones (AZs). Physiological studies have established that ethylene and auxin contribute to cell separation control. Genetic analyses of abscission mutants have defined ethylene-independent detachment regulators. Functional genomic strategies leading to global understandings of abscission have awaited methods for isolating AZ cells of low abundance and very small size. Here, we couple laser capture microdissection of <italic>Arabidopsis thaliana</italic>
stamen AZs and GeneChip profiling to reveal the AZ transcriptome responding to a developmental shedding cue. Analyses focus on 551 AZ genes (AZ<sub>551</sub>
) regulated at the highest statistical significance (<italic>P</italic>
≤ 0.0001) over five floral stages linking prepollination to stamen shed. AZ<sub>551</sub>
includes mediators of ethylene and auxin signaling as well as receptor-like kinases and extracellular ligands thought to act independent of ethylene. We hypothesized that novel abscission regulators might reside in disproportionately represented Gene Ontology Consortium functional categories for cell wall modifying proteins, extracellular regulators, and nuclear-residing transcription factors. Promoter-<italic>β</italic>
-glucuronidase expression of one transcription factor candidate, <italic>ZINC FINGER PROTEIN2</italic>
(<italic>AtZFP2</italic>
), was elevated in stamen, petal, and sepal AZs. Flower parts of transgenic lines overexpressing <italic>AtZFP2</italic>
exhibited asynchronous and delayed abscission. Abscission defects were accompanied by altered floral morphology limiting pollination and fertility. Hand-pollination restored transgenic fruit development but not the rapid abscission seen in wild-type plants, demonstrating that pollination does not assure normal rates of detachment. In wild-type stamen AZs, <italic>AtZFP2</italic>
is significantly up-regulated postanthesis. Phenotype data from transgene overexpression studies suggest that AtZFP2 participates in processes that directly or indirectly influence organ shed.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id>
<journal-title>Plant Physiology</journal-title>
<issn pub-type="ppub">0032-0889</issn>
<issn pub-type="epub">1532-2548</issn>
<publisher><publisher-name>American Society of Plant Biologists</publisher-name>
</publisher>
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<article-id pub-id-type="publisher-id">110908</article-id>
<article-id pub-id-type="doi">10.1104/pp.107.110908</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
<subj-group><subject>Systems Biology, Molecular Biology, and Gene Regulation</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis <italic>ZINC FINGER PROTEIN2</italic>
<xref ref-type="fn" rid="fn1">1</xref>
<xref ref-type="fn" rid="fn2">[C]</xref>
<xref ref-type="fn" rid="fn3">[W]</xref>
<xref ref-type="fn" rid="fn4">[OA]</xref>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Cai</surname>
<given-names>Suqin</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Lashbrook</surname>
<given-names>Coralie C.</given-names>
</name>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
</contrib-group>
<aff id="N0x34d9d20N0x1e22918">Department of Horticulture (S.C., C.C.L.), Interdepartmental Genetics Program (S.C., C.C.L.), and Plant Sciences Institute (C.C.L.), Iowa State University, Ames, Iowa 50011–1100</aff>
<author-notes><fn id="cor1"><label>*</label>
<p>Corresponding author; e-mail <email>ccl@iastate.edu</email>
.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>3</month>
<year>2008</year>
</pub-date>
<volume>146</volume>
<issue>3</issue>
<fpage>1305</fpage>
<lpage>1321</lpage>
<history><date date-type="received"><day>15</day>
<month>10</month>
<year>2007</year>
</date>
<date date-type="accepted"><day>8</day>
<month>1</month>
<year>2008</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2008, American Society of Plant Biologists</copyright-statement>
</permissions>
<abstract><p>Organ detachment requires cell separation within abscission zones (AZs). Physiological studies have established that ethylene and auxin contribute to cell separation control. Genetic analyses of abscission mutants have defined ethylene-independent detachment regulators. Functional genomic strategies leading to global understandings of abscission have awaited methods for isolating AZ cells of low abundance and very small size. Here, we couple laser capture microdissection of <italic>Arabidopsis thaliana</italic>
stamen AZs and GeneChip profiling to reveal the AZ transcriptome responding to a developmental shedding cue. Analyses focus on 551 AZ genes (AZ<sub>551</sub>
) regulated at the highest statistical significance (<italic>P</italic>
≤ 0.0001) over five floral stages linking prepollination to stamen shed. AZ<sub>551</sub>
includes mediators of ethylene and auxin signaling as well as receptor-like kinases and extracellular ligands thought to act independent of ethylene. We hypothesized that novel abscission regulators might reside in disproportionately represented Gene Ontology Consortium functional categories for cell wall modifying proteins, extracellular regulators, and nuclear-residing transcription factors. Promoter-<italic>β</italic>
-glucuronidase expression of one transcription factor candidate, <italic>ZINC FINGER PROTEIN2</italic>
(<italic>AtZFP2</italic>
), was elevated in stamen, petal, and sepal AZs. Flower parts of transgenic lines overexpressing <italic>AtZFP2</italic>
exhibited asynchronous and delayed abscission. Abscission defects were accompanied by altered floral morphology limiting pollination and fertility. Hand-pollination restored transgenic fruit development but not the rapid abscission seen in wild-type plants, demonstrating that pollination does not assure normal rates of detachment. In wild-type stamen AZs, <italic>AtZFP2</italic>
is significantly up-regulated postanthesis. Phenotype data from transgene overexpression studies suggest that AtZFP2 participates in processes that directly or indirectly influence organ shed.</p>
</abstract>
</article-meta>
<notes><fn-group><fn id="fn1"><label>1</label>
<p>This work was supported by Iowa State University (ISU) Agricultural Experiment Station, ISU Plant Sciences Institute, ISU College of Agriculture, and the U.S. Environmental Protection Agency (Cooperative Agreement no. CR–83281101).</p>
</fn>
<fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org">www.plantphysiol.org</ext-link>
) is: Coralie C. Lashbrook (<email>ccl@iastate.edu</email>
).</p>
</fn>
<fn id="fn2"><label>[C]</label>
<p>Some figures in this article are displayed in color online but in black and white in the print edition.</p>
</fn>
<fn id="fn3"><label>[W]</label>
<p>The online version of this article contains Web-only data.</p>
</fn>
<fn id="fn4"><label>[OA]</label>
<p>Open Access articles can be viewed online without a subscription.</p>
</fn>
<fn><p><ext-link ext-link-type="uri" xlink:href="www.plantphysiol.org/cgi/doi/10.1104/pp.107.110908">www.plantphysiol.org/cgi/doi/10.1104/pp.107.110908</ext-link>
</p>
</fn>
</fn-group>
</notes>
</front>
</pmc>
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<name sortKey="Lashbrook, Coralie C" sort="Lashbrook, Coralie C" uniqKey="Lashbrook C" first="Coralie C." last="Lashbrook">Coralie C. Lashbrook</name>
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