Fungicide resistance and genetic variability in plant pathogenic strains of Guignardia citricarpa
Identifieur interne : 001019 ( Pmc/Checkpoint ); précédent : 001018; suivant : 001020Fungicide resistance and genetic variability in plant pathogenic strains of Guignardia citricarpa
Auteurs : Y. M. Possiede [Brésil] ; J. Gabardo [Brésil] ; V. Kava-Cordeiro [Brésil] ; L. V. Galli-Terasawa [Brésil] ; J. L. Azevedo [Brésil] ; C. Glienke [Brésil]Source :
- Brazilian Journal of Microbiology [ 1517-8382 ] ; 2009.
Abstract
Citrus black spot (CBS) is a plant disease of worldwide occurrence, affecting crops in Africa, Oceania, and South America. In Brazil, climate provides favorable conditions and CBS has spread to the Southeast and South regions. CBS is caused by the fungus
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DOI: 10.1590/S1517-838220090002000018
PubMed: 24031363
PubMed Central: 3769719
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fungicide resistance and genetic variability in plant pathogenic strains of <italic>Guignardia citricarpa</italic></title><author><name sortKey="Possiede, Y M" sort="Possiede, Y M" uniqKey="Possiede Y" first="Y. M." last="Possiede">Y. M. Possiede</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Departamento de Biologia, Universidade Federal de Mato Grosso do Sul</institution>,<addr-line>Campo Grande, MS</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gabardo, J" sort="Gabardo, J" uniqKey="Gabardo J" first="J." last="Gabardo">J. Gabardo</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kava Cordeiro, V" sort="Kava Cordeiro, V" uniqKey="Kava Cordeiro V" first="V." last="Kava-Cordeiro">V. Kava-Cordeiro</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Galli Terasawa, L V" sort="Galli Terasawa, L V" uniqKey="Galli Terasawa L" first="L. V." last="Galli-Terasawa">L. V. Galli-Terasawa</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Azevedo, J L" sort="Azevedo, J L" uniqKey="Azevedo J" first="J. L." last="Azevedo">J. L. Azevedo</name><affiliation wicri:level="1"><nlm:aff id="au3"><institution>Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo</institution>,<addr-line>Piracicaba, SP</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Glienke, C" sort="Glienke, C" uniqKey="Glienke C" first="C." last="Glienke">C. Glienke</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24031363</idno><idno type="pmc">3769719</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769719</idno><idno type="RBID">PMC:3769719</idno><idno type="doi">10.1590/S1517-838220090002000018</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000E58</idno><idno type="wicri:Area/Pmc/Curation">000E57</idno><idno type="wicri:Area/Pmc/Checkpoint">001019</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Fungicide resistance and genetic variability in plant pathogenic strains of <italic>Guignardia citricarpa</italic></title><author><name sortKey="Possiede, Y M" sort="Possiede, Y M" uniqKey="Possiede Y" first="Y. M." last="Possiede">Y. M. Possiede</name><affiliation wicri:level="1"><nlm:aff id="au1"><institution>Departamento de Biologia, Universidade Federal de Mato Grosso do Sul</institution>,<addr-line>Campo Grande, MS</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gabardo, J" sort="Gabardo, J" uniqKey="Gabardo J" first="J." last="Gabardo">J. Gabardo</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kava Cordeiro, V" sort="Kava Cordeiro, V" uniqKey="Kava Cordeiro V" first="V." last="Kava-Cordeiro">V. Kava-Cordeiro</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Galli Terasawa, L V" sort="Galli Terasawa, L V" uniqKey="Galli Terasawa L" first="L. V." last="Galli-Terasawa">L. V. Galli-Terasawa</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Azevedo, J L" sort="Azevedo, J L" uniqKey="Azevedo J" first="J. L." last="Azevedo">J. L. Azevedo</name><affiliation wicri:level="1"><nlm:aff id="au3"><institution>Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo</institution>,<addr-line>Piracicaba, SP</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Glienke, C" sort="Glienke, C" uniqKey="Glienke C" first="C." last="Glienke">C. Glienke</name><affiliation wicri:level="1"><nlm:aff id="au2"><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Brazilian Journal of Microbiology</title><idno type="ISSN">1517-8382</idno><idno type="eISSN">1678-4405</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Citrus black spot (CBS) is a plant disease of worldwide occurrence, affecting crops in Africa, Oceania, and South America. In Brazil, climate provides favorable conditions and CBS has spread to the Southeast and South regions. CBS is caused by the fungus <italic>Guignardia citricarpa</italic> (anamorph: <italic>Phyllosticta citricarpa</italic>) and its control is based on the use of fungicides, such as benzimidazoles. In South Africa, the disease was kept under control for 10 years with benomyl, until cases of resistance to high concentrations of this fungicide were reported from all citrus-producing areas. Azoxystrobin (a strobilurin) has been found effective in controlling phytopathogens, including CBS, in a wide range of economically important crops. The present study investigated <italic>in vitro</italic> the effects of the fungicides benomyl and azoxystrobin on 10 strains of <italic>G. citricarpa</italic> isolated from lesions in citrus plants from Brazil and South Africa. Benomyl at 0.5 μg/mL inhibited mycelial growth in all strains except PC3C, of African origin, which exhibited resistance to concentrations of up to 100.0 μg/mL. The spontaneous mutation frequency for resistance to benomyl was 1.25 × 10<sup>-7</sup>. Azoxystrobin, even at high concentrations, did not inhibit mycelial growth in any of the strains, but significantly reduced sporulation rates, by as much as 100%, at a concentration of 5.0 μg/mL. Variations in sensitivity across strains, particularly to the strobilurin azoxystrobin, are possibly related to genetic variability in <italic>G. citricarpa</italic> isolates.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Azevedo, J L" uniqKey="Azevedo J">J.L. Azevedo</name></author><author><name sortKey="Costa, S O P" uniqKey="Costa S">S.O.P. Costa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baayen, R P" uniqKey="Baayen R">R.P. Baayen</name></author><author><name sortKey="Bonants, P J M" uniqKey="Bonants P">P.J.M. Bonants</name></author><author><name sortKey="Verkley, G" uniqKey="Verkley G">G. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Braz J Microbiol</journal-id><journal-id journal-id-type="iso-abbrev">Braz. J. Microbiol</journal-id><journal-id journal-id-type="publisher-id">bjm</journal-id><journal-id journal-id-type="pmc">bjm</journal-id><journal-title-group><journal-title>Brazilian Journal of Microbiology</journal-title></journal-title-group><issn pub-type="ppub">1517-8382</issn><issn pub-type="epub">1678-4405</issn><publisher><publisher-name>Sociedade Brasileira de Microbiologia</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24031363</article-id><article-id pub-id-type="pmc">3769719</article-id><article-id pub-id-type="publisher-id">S1517-838220090002000018</article-id><article-id pub-id-type="doi">10.1590/S1517-838220090002000018</article-id><article-categories><subj-group subj-group-type="heading"><subject>Environmental Microbiology</subject><subj-group><subject>Research Paper</subject></subj-group></subj-group></article-categories><title-group><article-title>Fungicide resistance and genetic variability in plant pathogenic strains of <italic>Guignardia citricarpa</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Possiede</surname><given-names>Y.M.</given-names></name><xref ref-type="aff" rid="au1"><sup>1</sup></xref><xref ref-type="aff" rid="au2"><sup>2</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gabardo</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="au2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kava-Cordeiro</surname><given-names>V.</given-names></name><xref ref-type="aff" rid="au2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Galli-Terasawa</surname><given-names>L.V.</given-names></name><xref ref-type="aff" rid="au2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Azevedo</surname><given-names>J.L.</given-names></name><xref ref-type="aff" rid="au3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Glienke</surname><given-names>C.</given-names></name><xref ref-type="aff" rid="au2"><sup>2</sup></xref></contrib></contrib-group><aff id="au1"><label>1</label><institution>Departamento de Biologia, Universidade Federal de Mato Grosso do Sul</institution>,<addr-line>Campo Grande, MS</addr-line>,<country>Brasil</country></aff><aff id="au2"><label>2</label><institution>Departamento de Genética, Universidade Federal do Paraná</institution>,<addr-line>Curitiba, PR</addr-line>,<country>Brasil</country></aff><aff id="au3"><label>3</label><institution>Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo</institution>,<addr-line>Piracicaba, SP</addr-line>,<country>Brasil</country></aff><author-notes><corresp id="cor1"><sup>*</sup>Corresponding Author. Mailing address: Departamento de Biologia, UFMS. Caixa Postal 549, Campo Grande, MS 79070-900, Brazil. Tel./fax (67) 3345-7322. E-mail: <email>ympossiede@gmail.com</email></corresp></author-notes><pub-date pub-type="ppub"><season>Apr-Jun</season><year>2009</year></pub-date><pub-date pub-type="epub"><day>1</day><month>6</month><year>2009</year></pub-date><volume>40</volume><issue>2</issue><fpage>308</fpage><lpage>313</lpage><history><date date-type="received"><day>20</day><month>6</month><year>2008</year></date><date date-type="rev-recd"><day>05</day><month>9</month><year>2008</year></date><date date-type="accepted"><day>31</day><month>3</month><year>2009</year></date></history><permissions><copyright-statement>© Sociedade Brasileira de Microbiologia</copyright-statement><copyright-year>2009</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p>All the content of the journal, except where otherwise noted, is licensed under a <underline>Creative Commons License</underline></license-p></license></permissions><abstract><p>Citrus black spot (CBS) is a plant disease of worldwide occurrence, affecting crops in Africa, Oceania, and South America. In Brazil, climate provides favorable conditions and CBS has spread to the Southeast and South regions. CBS is caused by the fungus <italic>Guignardia citricarpa</italic> (anamorph: <italic>Phyllosticta citricarpa</italic>) and its control is based on the use of fungicides, such as benzimidazoles. In South Africa, the disease was kept under control for 10 years with benomyl, until cases of resistance to high concentrations of this fungicide were reported from all citrus-producing areas. Azoxystrobin (a strobilurin) has been found effective in controlling phytopathogens, including CBS, in a wide range of economically important crops. The present study investigated <italic>in vitro</italic> the effects of the fungicides benomyl and azoxystrobin on 10 strains of <italic>G. citricarpa</italic> isolated from lesions in citrus plants from Brazil and South Africa. Benomyl at 0.5 μg/mL inhibited mycelial growth in all strains except PC3C, of African origin, which exhibited resistance to concentrations of up to 100.0 μg/mL. The spontaneous mutation frequency for resistance to benomyl was 1.25 × 10<sup>-7</sup>. Azoxystrobin, even at high concentrations, did not inhibit mycelial growth in any of the strains, but significantly reduced sporulation rates, by as much as 100%, at a concentration of 5.0 μg/mL. Variations in sensitivity across strains, particularly to the strobilurin azoxystrobin, are possibly related to genetic variability in <italic>G. citricarpa</italic> isolates.</p></abstract><kwd-group><kwd><italic>G. citricarpa</italic></kwd><kwd>Azoxystrobin</kwd><kwd>Benomyl</kwd><kwd>Citrus black spot</kwd><kwd>Fungicides</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Brésil</li></country></list><tree><country name="Brésil"><noRegion><name sortKey="Possiede, Y M" sort="Possiede, Y M" uniqKey="Possiede Y" first="Y. M." last="Possiede">Y. M. Possiede</name></noRegion><name sortKey="Azevedo, J L" sort="Azevedo, J L" uniqKey="Azevedo J" first="J. L." last="Azevedo">J. L. Azevedo</name><name sortKey="Gabardo, J" sort="Gabardo, J" uniqKey="Gabardo J" first="J." last="Gabardo">J. Gabardo</name><name sortKey="Galli Terasawa, L V" sort="Galli Terasawa, L V" uniqKey="Galli Terasawa L" first="L. V." last="Galli-Terasawa">L. V. Galli-Terasawa</name><name sortKey="Glienke, C" sort="Glienke, C" uniqKey="Glienke C" first="C." last="Glienke">C. Glienke</name><name sortKey="Kava Cordeiro, V" sort="Kava Cordeiro, V" uniqKey="Kava Cordeiro V" first="V." last="Kava-Cordeiro">V. Kava-Cordeiro</name><name sortKey="Possiede, Y M" sort="Possiede, Y M" uniqKey="Possiede Y" first="Y. M." last="Possiede">Y. M. Possiede</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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