Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence
Identifieur interne : 001000 ( Pmc/Checkpoint ); précédent : 000F99; suivant : 001001Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence
Auteurs : Pablo Aleza [Espagne] ; José Juárez [Espagne] ; María Hernández [Espagne] ; José A. Pina [Espagne] ; Patrick Ollitrault [France] ; Luis Navarro [Espagne]Source :
- BMC Plant Biology [ 1471-2229 ] ; 2009.
Abstract
In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a homozygous plant. Due to the existence of important molecular resources and previous success in obtaining haploid clementine plants, haploid clementine was selected as the target for the implementation of the reference whole genome citrus sequence.
To obtain haploid clementine lines we used the technique of
The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus.
Url:
DOI: 10.1186/1471-2229-9-110
PubMed: 19698121
PubMed Central: 2747335
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:2747335Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Recovery and characterization of a <italic>Citrus clementina </italic>
Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence</title>
<author><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hernandez, Maria" sort="Hernandez, Maria" uniqKey="Hernandez M" first="María" last="Hernández">María Hernández</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pina, Jose A" sort="Pina, Jose A" uniqKey="Pina J" first="José A" last="Pina">José A. Pina</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name>
<affiliation wicri:level="3"><nlm:aff id="I2">Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19698121</idno>
<idno type="pmc">2747335</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747335</idno>
<idno type="RBID">PMC:2747335</idno>
<idno type="doi">10.1186/1471-2229-9-110</idno>
<date when="2009">2009</date>
<idno type="wicri:Area/Pmc/Corpus">000B32</idno>
<idno type="wicri:Area/Pmc/Curation">000B31</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001000</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Recovery and characterization of a <italic>Citrus clementina </italic>
Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence</title>
<author><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hernandez, Maria" sort="Hernandez, Maria" uniqKey="Hernandez M" first="María" last="Hernández">María Hernández</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pina, Jose A" sort="Pina, Jose A" uniqKey="Pina J" first="José A" last="Pina">José A. Pina</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name>
<affiliation wicri:level="3"><nlm:aff id="I2">Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name>
<affiliation wicri:level="1"><nlm:aff id="I1">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia</wicri:regionArea>
<wicri:noRegion>Valencia</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Plant Biology</title>
<idno type="eISSN">1471-2229</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a homozygous plant. Due to the existence of important molecular resources and previous success in obtaining haploid clementine plants, haploid clementine was selected as the target for the implementation of the reference whole genome citrus sequence.</p>
</sec>
<sec><title>Results</title>
<p>To obtain haploid clementine lines we used the technique of <italic>in situ </italic>
gynogenesis induced by irradiated pollen. Flow cytometry, chromosome counts and SSR marker (Simple Sequence Repeats) analysis facilitated the identification of six different haploid lines (2<italic>n </italic>
= <italic>x </italic>
= 9), one aneuploid line (2<italic>n </italic>
= 2<italic>x</italic>
+4 = 22) and one doubled haploid plant (2<italic>n </italic>
= 2<italic>x </italic>
= 18) of 'Clemenules' clementine. One of the haploids, obtained directly from an original haploid embryo, grew vigorously and produced flowers after four years. This is the first haploid plant of clementine that has bloomed and we have, for the first time, characterized the histology of haploid and diploid flowers of clementine. Additionally a double haploid plant was obtained spontaneously from this haploid line.</p>
</sec>
<sec><title>Conclusion</title>
<p>The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Germana, Ma" uniqKey="Germana M">MA Germanà</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tal N, M" uniqKey="Tal N M">M Talón</name>
</author>
<author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Forster, Bp" uniqKey="Forster B">BP Forster</name>
</author>
<author><name sortKey="Erwin, Hb" uniqKey="Erwin H">HB Erwin</name>
</author>
<author><name sortKey="Ken, Jk" uniqKey="Ken J">JK Ken</name>
</author>
<author><name sortKey="Alisher, T" uniqKey="Alisher T">T Alisher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leeuwen, H" uniqKey="Leeuwen H">H Leeuwen</name>
</author>
<author><name sortKey="Monfort, A" uniqKey="Monfort A">A Monfort</name>
</author>
<author><name sortKey="Zhang, H" uniqKey="Zhang H">H Zhang</name>
</author>
<author><name sortKey="Puigdomenech, P" uniqKey="Puigdomenech P">P Puigdomènech</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hussain, T" uniqKey="Hussain T">T Hussain</name>
</author>
<author><name sortKey="Tausend, P" uniqKey="Tausend P">P Tausend</name>
</author>
<author><name sortKey="Graham, G" uniqKey="Graham G">G Graham</name>
</author>
<author><name sortKey="Ho, J" uniqKey="Ho J">J Ho</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, Kp" uniqKey="Zhang K">KP Zhang</name>
</author>
<author><name sortKey="Zhao, L" uniqKey="Zhao L">L Zhao</name>
</author>
<author><name sortKey="Tian, Jc" uniqKey="Tian J">JC Tian</name>
</author>
<author><name sortKey="Chen, Gf" uniqKey="Chen G">GF Chen</name>
</author>
<author><name sortKey="Jiang, Xl" uniqKey="Jiang X">XL Jiang</name>
</author>
<author><name sortKey="Liu, B" uniqKey="Liu B">B Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chu, Cg" uniqKey="Chu C">CG Chu</name>
</author>
<author><name sortKey="Xu, Ss" uniqKey="Xu S">SS Xu</name>
</author>
<author><name sortKey="Friesen, Tl" uniqKey="Friesen T">TL Friesen</name>
</author>
<author><name sortKey="Faris, Jd" uniqKey="Faris J">JD Faris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="King, G" uniqKey="King G">G King</name>
</author>
<author><name sortKey="Bennett, M" uniqKey="Bennett M">M Bennett</name>
</author>
<author><name sortKey="May, S" uniqKey="May S">S May</name>
</author>
<author><name sortKey="Mcewan, G" uniqKey="Mcewan G">G McEwan</name>
</author>
<author><name sortKey="Ryder, Cd" uniqKey="Ryder C">CD Ryder</name>
</author>
<author><name sortKey="Sarjeant, A" uniqKey="Sarjeant A">A Sarjeant</name>
</author>
<author><name sortKey="Smith, Lb" uniqKey="Smith L">LB Smith</name>
</author>
<author><name sortKey="Teakle, Gr" uniqKey="Teakle G">GR Teakle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kunzel, Gl" uniqKey="Kunzel G">GL Kunzel</name>
</author>
<author><name sortKey="Korzun, L" uniqKey="Korzun L">L Korzun</name>
</author>
<author><name sortKey="Meister, A" uniqKey="Meister A">A Meister</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Z" uniqKey="Wang Z">Z Wang</name>
</author>
<author><name sortKey="Taramino, G" uniqKey="Taramino G">G Taramino</name>
</author>
<author><name sortKey="Yang, D" uniqKey="Yang D">D Yang</name>
</author>
<author><name sortKey="Liu, G" uniqKey="Liu G">G Liu</name>
</author>
<author><name sortKey="Tingey, Sv" uniqKey="Tingey S">SV Tingey</name>
</author>
<author><name sortKey="Miao, Gh" uniqKey="Miao G">GH Miao</name>
</author>
<author><name sortKey="Wang, Gl" uniqKey="Wang G">GL Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Szareijo, I" uniqKey="Szareijo I">I Szareijo</name>
</author>
<author><name sortKey="Forster, Bp" uniqKey="Forster B">BP Forster</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moroldo, M" uniqKey="Moroldo M">M Moroldo</name>
</author>
<author><name sortKey="Paillard, S" uniqKey="Paillard S">S Paillard</name>
</author>
<author><name sortKey="Marconi, R" uniqKey="Marconi R">R Marconi</name>
</author>
<author><name sortKey="Fabrice, L" uniqKey="Fabrice L">L Fabrice</name>
</author>
<author><name sortKey="Canaguier, A" uniqKey="Canaguier A">A Canaguier</name>
</author>
<author><name sortKey="Cruaud, C" uniqKey="Cruaud C">C Cruaud</name>
</author>
<author><name sortKey="De Berardinis, V" uniqKey="De Berardinis V">V De Berardinis</name>
</author>
<author><name sortKey="Guichard, C" uniqKey="Guichard C">C Guichard</name>
</author>
<author><name sortKey="Brunaud, V" uniqKey="Brunaud V">V Brunaud</name>
</author>
<author><name sortKey="Le Clainche, I" uniqKey="Le Clainche I">I Le Clainche</name>
</author>
<author><name sortKey="Scalabrin, S" uniqKey="Scalabrin S">S Scalabrin</name>
</author>
<author><name sortKey="Testolin, R" uniqKey="Testolin R">R Testolin</name>
</author>
<author><name sortKey="Di Gaspero, G" uniqKey="Di Gaspero G">G Di Gaspero</name>
</author>
<author><name sortKey="Morgante, M" uniqKey="Morgante M">M Morgante</name>
</author>
<author><name sortKey="Adam Blondon, Af" uniqKey="Adam Blondon A">AF Adam-Blondon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vinson, Jp" uniqKey="Vinson J">JP Vinson</name>
</author>
<author><name sortKey="Jaffe, Db" uniqKey="Jaffe D">DB Jaffe</name>
</author>
<author><name sortKey="O Neill, K" uniqKey="O Neill K">K O'Neill</name>
</author>
<author><name sortKey="Karlsson, Ek" uniqKey="Karlsson E">EK Karlsson</name>
</author>
<author><name sortKey="Stange Thomann, N" uniqKey="Stange Thomann N">N Stange-Thomann</name>
</author>
<author><name sortKey="Anderson, S" uniqKey="Anderson S">S Anderson</name>
</author>
<author><name sortKey="Mesirov, Jp" uniqKey="Mesirov J">JP Mesirov</name>
</author>
<author><name sortKey="Satoh, N" uniqKey="Satoh N">N Satoh</name>
</author>
<author><name sortKey="Satou, Y" uniqKey="Satou Y">Y Satou</name>
</author>
<author><name sortKey="Nusbaum, C" uniqKey="Nusbaum C">C Nusbaum</name>
</author>
<author><name sortKey="Birren, B" uniqKey="Birren B">B Birren</name>
</author>
<author><name sortKey="Galagan, Je" uniqKey="Galagan J">JE Galagan</name>
</author>
<author><name sortKey="Lander, Es" uniqKey="Lander E">ES Lander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bronner, A" uniqKey="Bronner A">A Bronner</name>
</author>
<author><name sortKey="Oliveira, J" uniqKey="Oliveira J">J Oliveira</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Herrero, R" uniqKey="Herrero R">R Herrero</name>
</author>
<author><name sortKey="Asins, Mj" uniqKey="Asins M">MJ Asins</name>
</author>
<author><name sortKey="Carbonell, E" uniqKey="Carbonell E">E Carbonell</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
<author><name sortKey="Niedz, Rp" uniqKey="Niedz R">RP Niedz</name>
</author>
<author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name>
</author>
<author><name sortKey="Timothy, Jc" uniqKey="Timothy J">JC Timothy</name>
</author>
<author><name sortKey="Abhaya, Md" uniqKey="Abhaya M">MD Abhaya</name>
</author>
<author><name sortKey="Jan Fang, C" uniqKey="Jan Fang C">C Jan-Fang</name>
</author>
<author><name sortKey="Daniel, Sr" uniqKey="Daniel S">SR Daniel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Forment, J" uniqKey="Forment J">J Forment</name>
</author>
<author><name sortKey="Gadea, J" uniqKey="Gadea J">J Gadea</name>
</author>
<author><name sortKey="Huerta, L" uniqKey="Huerta L">L Huerta</name>
</author>
<author><name sortKey="Abizanda, L" uniqKey="Abizanda L">L Abizanda</name>
</author>
<author><name sortKey="Agusti, J" uniqKey="Agusti J">J Agusti</name>
</author>
<author><name sortKey="Alamar, S" uniqKey="Alamar S">S Alamar</name>
</author>
<author><name sortKey="Alos, E" uniqKey="Alos E">E Alos</name>
</author>
<author><name sortKey="Andres, F" uniqKey="Andres F">F Andres</name>
</author>
<author><name sortKey="Arribas, R" uniqKey="Arribas R">R Arribas</name>
</author>
<author><name sortKey="Beltran, Jp" uniqKey="Beltran J">JP Beltran</name>
</author>
<author><name sortKey="Berbel, A" uniqKey="Berbel A">A Berbel</name>
</author>
<author><name sortKey="Blazquez, Ma" uniqKey="Blazquez M">MA Blazquez</name>
</author>
<author><name sortKey="Brumos, J" uniqKey="Brumos J">J Brumos</name>
</author>
<author><name sortKey="Canas, La" uniqKey="Canas L">LA Canas</name>
</author>
<author><name sortKey="Cercos, M" uniqKey="Cercos M">M Cercos</name>
</author>
<author><name sortKey="Colmenero Flores, Jm" uniqKey="Colmenero Flores J">JM Colmenero-Flores</name>
</author>
<author><name sortKey="Conesa, A" uniqKey="Conesa A">A Conesa</name>
</author>
<author><name sortKey="Estables, B" uniqKey="Estables B">B Estables</name>
</author>
<author><name sortKey="Gandia, M" uniqKey="Gandia M">M Gandia</name>
</author>
<author><name sortKey="Garcia Martinez, Jl" uniqKey="Garcia Martinez J">JL Garcia-Martinez</name>
</author>
<author><name sortKey="Gimeno, J" uniqKey="Gimeno J">J Gimeno</name>
</author>
<author><name sortKey="Gisbert, A" uniqKey="Gisbert A">A Gisbert</name>
</author>
<author><name sortKey="Gomez, G" uniqKey="Gomez G">G Gomez</name>
</author>
<author><name sortKey="Gonzalez Candelas, L" uniqKey="Gonzalez Candelas L">L Gonzalez-Candelas</name>
</author>
<author><name sortKey="Granell, A" uniqKey="Granell A">A Granell</name>
</author>
<author><name sortKey="Guerri, J" uniqKey="Guerri J">J Guerri</name>
</author>
<author><name sortKey="Lafuente, Mt" uniqKey="Lafuente M">MT Lafuente</name>
</author>
<author><name sortKey="Madueno, F" uniqKey="Madueno F">F Madueno</name>
</author>
<author><name sortKey="Marcos, Jf" uniqKey="Marcos J">JF Marcos</name>
</author>
<author><name sortKey="Marques, Mc" uniqKey="Marques M">MC Marques</name>
</author>
<author><name sortKey="Martinez, F" uniqKey="Martinez F">F Martinez</name>
</author>
<author><name sortKey="Martinez Godoy, Ma" uniqKey="Martinez Godoy M">MA Martinez-Godoy</name>
</author>
<author><name sortKey="Miralles, S" uniqKey="Miralles S">S Miralles</name>
</author>
<author><name sortKey="Moreno, P" uniqKey="Moreno P">P Moreno</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Pallas, V" uniqKey="Pallas V">V Pallas</name>
</author>
<author><name sortKey="Perez Amador, Ma" uniqKey="Perez Amador M">MA Perez-Amador</name>
</author>
<author><name sortKey="Perez Valle, J" uniqKey="Perez Valle J">J Perez-Valle</name>
</author>
<author><name sortKey="Pons, C" uniqKey="Pons C">C Pons</name>
</author>
<author><name sortKey="Rodrigo, I" uniqKey="Rodrigo I">I Rodrigo</name>
</author>
<author><name sortKey="Rodriguez, Pl" uniqKey="Rodriguez P">PL Rodriguez</name>
</author>
<author><name sortKey="Royo, C" uniqKey="Royo C">C Royo</name>
</author>
<author><name sortKey="Serrano, R" uniqKey="Serrano R">R Serrano</name>
</author>
<author><name sortKey="Soler, G" uniqKey="Soler G">G Soler</name>
</author>
<author><name sortKey="Tadeo, F" uniqKey="Tadeo F">F Tadeo</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
<author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Trenor, M" uniqKey="Trenor M">M Trenor</name>
</author>
<author><name sortKey="Vaello, L" uniqKey="Vaello L">L Vaello</name>
</author>
<author><name sortKey="Vicente, O" uniqKey="Vicente O">O Vicente</name>
</author>
<author><name sortKey="Vidal, C" uniqKey="Vidal C">C Vidal</name>
</author>
<author><name sortKey="Zacarias, L" uniqKey="Zacarias L">L Zacarias</name>
</author>
<author><name sortKey="Conejero, V" uniqKey="Conejero V">V Conejero</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Terol, Jf" uniqKey="Terol J">JF Terol</name>
</author>
<author><name sortKey="Argout, X" uniqKey="Argout X">X Argout</name>
</author>
<author><name sortKey="Allario, T" uniqKey="Allario T">T Allario</name>
</author>
<author><name sortKey="Wincker, P" uniqKey="Wincker P">P Wincker</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Conesa, A" uniqKey="Conesa A">A Conesa</name>
</author>
<author><name sortKey="Colmenero, Jm" uniqKey="Colmenero J">JM Colmenero</name>
</author>
<author><name sortKey="Cercos, M" uniqKey="Cercos M">M Cercos</name>
</author>
<author><name sortKey="Tadeo, Fr" uniqKey="Tadeo F">FR Tadeo</name>
</author>
<author><name sortKey="Agusti, J" uniqKey="Agusti J">J Agustí</name>
</author>
<author><name sortKey="Al S, E" uniqKey="Al S E">E Alós</name>
</author>
<author><name sortKey="Andres, F" uniqKey="Andres F">F Andres</name>
</author>
<author><name sortKey="Soler, G" uniqKey="Soler G">G Soler</name>
</author>
<author><name sortKey="Brumos, J" uniqKey="Brumos J">J Brumos</name>
</author>
<author><name sortKey="Iglesias, Dj" uniqKey="Iglesias D">DJ Iglesias</name>
</author>
<author><name sortKey="Gotz, S" uniqKey="Gotz S">S Götz</name>
</author>
<author><name sortKey="Legaz, F" uniqKey="Legaz F">F Legaz</name>
</author>
<author><name sortKey="Argout, X" uniqKey="Argout X">X Argout</name>
</author>
<author><name sortKey="Courtois, B" uniqKey="Courtois B">B Courtois</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Dossat, C" uniqKey="Dossat C">C Dossat</name>
</author>
<author><name sortKey="Wincker, P" uniqKey="Wincker P">P Wincker</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Martinez Godoy, Ma" uniqKey="Martinez Godoy M">MA Martinez-Godoy</name>
</author>
<author><name sortKey="Mauri, N" uniqKey="Mauri N">N Mauri</name>
</author>
<author><name sortKey="Juarez, J" uniqKey="Juarez J">J Juarez</name>
</author>
<author><name sortKey="Marques, Mc" uniqKey="Marques M">MC Marques</name>
</author>
<author><name sortKey="Santiago, J" uniqKey="Santiago J">J Santiago</name>
</author>
<author><name sortKey="Forment, J" uniqKey="Forment J">J Forment</name>
</author>
<author><name sortKey="Gadea, J" uniqKey="Gadea J">J Gadea</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Terol, Jm" uniqKey="Terol J">JM Terol</name>
</author>
<author><name sortKey="Naranjo, A" uniqKey="Naranjo A">A Naranjo</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Billot, C" uniqKey="Billot C">C Billot</name>
</author>
<author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
<author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name>
</author>
<author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Lotfy, S" uniqKey="Lotfy S">S Lotfy</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name>
</author>
<author><name sortKey="Mu, L" uniqKey="Mu L">L Mu</name>
</author>
<author><name sortKey="Chen, Cx" uniqKey="Chen C">CX Chen</name>
</author>
<author><name sortKey="Kacar, Y" uniqKey="Kacar Y">Y Kacar</name>
</author>
<author><name sortKey="Ollitrault, F" uniqKey="Ollitrault F">F Ollitrault</name>
</author>
<author><name sortKey="Ferrer, Rm" uniqKey="Ferrer R">RM Ferrer</name>
</author>
<author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Billot, C" uniqKey="Billot C">C Billot</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
<author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Allent, V" uniqKey="Allent V">V Allent</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Germana, Ma" uniqKey="Germana M">MA Germanà</name>
</author>
<author><name sortKey="Chiancone, B" uniqKey="Chiancone B">B Chiancone</name>
</author>
<author><name sortKey="Lain, O" uniqKey="Lain O">O Lain</name>
</author>
<author><name sortKey="Testolin, R" uniqKey="Testolin R">R Testolin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drira, N" uniqKey="Drira N">N Drira</name>
</author>
<author><name sortKey="Benbadis, Ya" uniqKey="Benbadis Y">YA Benbadis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hidaka, T" uniqKey="Hidaka T">T Hidaka</name>
</author>
<author><name sortKey="Yamada, Y" uniqKey="Yamada Y">Y Yamada</name>
</author>
<author><name sortKey="Shichijo, T" uniqKey="Shichijo T">T Shichijo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Zq" uniqKey="Chen Z">ZQ Chen</name>
</author>
<author><name sortKey="Wang, Mq" uniqKey="Wang M">MQ Wang</name>
</author>
<author><name sortKey="Huihua, L" uniqKey="Huihua L">L Huihua</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hidaka, T" uniqKey="Hidaka T">T Hidaka</name>
</author>
<author><name sortKey="Kajiura, I" uniqKey="Kajiura I">I Kajiura</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Germana, Ma" uniqKey="Germana M">MA Germanà</name>
</author>
<author><name sortKey="Reforgiato Recupero, G" uniqKey="Reforgiato Recupero G">G Reforgiato-Recupero</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Deng, Xx" uniqKey="Deng X">XX Deng</name>
</author>
<author><name sortKey="Deng, Za" uniqKey="Deng Z">ZA Deng</name>
</author>
<author><name sortKey="Xiao, Sy" uniqKey="Xiao S">SY Xiao</name>
</author>
<author><name sortKey="Zhang, Wc" uniqKey="Zhang W">WC Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Germana, Ma" uniqKey="Germana M">MA Germanà</name>
</author>
<author><name sortKey="Crescimano, Fg" uniqKey="Crescimano F">FG Crescimano</name>
</author>
<author><name sortKey="Reforgiato Recupero, G" uniqKey="Reforgiato Recupero G">G Reforgiato-Recupero</name>
</author>
<author><name sortKey="Russo, Mp" uniqKey="Russo M">MP Russo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Germana, Ma" uniqKey="Germana M">MA Germanà</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Deng, Xx" uniqKey="Deng X">XX Deng</name>
</author>
<author><name sortKey="Hao, Yj" uniqKey="Hao Y">YJ Hao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grosser, Jw" uniqKey="Grosser J">JW Grosser</name>
</author>
<author><name sortKey="Deng, Xx" uniqKey="Deng X">XX Deng</name>
</author>
<author><name sortKey="Goodrich, Rm" uniqKey="Goodrich R">RM Goodrich</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pandey, Kk" uniqKey="Pandey K">KK Pandey</name>
</author>
<author><name sortKey="Przywara, L" uniqKey="Przywara L">L Przywara</name>
</author>
<author><name sortKey="Sanders, Pm" uniqKey="Sanders P">PM Sanders</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, Yx" uniqKey="Zhang Y">YX Zhang</name>
</author>
<author><name sortKey="Lespinasse, Y" uniqKey="Lespinasse Y">Y Lespinasse</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouvier, L" uniqKey="Bouvier L">L Bouvier</name>
</author>
<author><name sortKey="Zhang, Yx" uniqKey="Zhang Y">YX Zhang</name>
</author>
<author><name sortKey="Lespinasse, Y" uniqKey="Lespinasse Y">Y Lespinasse</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hofer, M" uniqKey="Hofer M">M Höfer</name>
</author>
<author><name sortKey="Grafe, Ch" uniqKey="Grafe C">CH Grafe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Esen, A" uniqKey="Esen A">A Esen</name>
</author>
<author><name sortKey="Soost, Rk" uniqKey="Soost R">RK Soost</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Toolapong, P" uniqKey="Toolapong P">P Toolapong</name>
</author>
<author><name sortKey="Komatsu, H" uniqKey="Komatsu H">H Komatsu</name>
</author>
<author><name sortKey="Iwamasa, M" uniqKey="Iwamasa M">M Iwamasa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oiyama, I" uniqKey="Oiyama I">I Oiyama</name>
</author>
<author><name sortKey="Kobayashi, S" uniqKey="Kobayashi S">S Kobayashi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Germana, Ma" uniqKey="Germana M">MA Germanà</name>
</author>
<author><name sortKey="Chiancone, B" uniqKey="Chiancone B">B Chiancone</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Bassene, Jb" uniqKey="Bassene J">JB Bassene</name>
</author>
<author><name sortKey="Jedidi Neji, E" uniqKey="Jedidi Neji E">E JedidI-Neji</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Bernardini, G" uniqKey="Bernardini G">G Bernardini</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Juarez, J" uniqKey="Juarez J">J Juárez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Caligari, Pds" uniqKey="Caligari P">PDS Caligari</name>
</author>
<author><name sortKey="Ingram, Nr" uniqKey="Ingram N">NR Ingram</name>
</author>
<author><name sortKey="Jinks, Jl" uniqKey="Jinks J">JL Jinks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pandey, Kk" uniqKey="Pandey K">KK Pandey</name>
</author>
<author><name sortKey="Phung, M" uniqKey="Phung M">M Phung</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Dambier, D" uniqKey="Dambier D">D Dambier</name>
</author>
<author><name sortKey="Vanel, F" uniqKey="Vanel F">F Vanel</name>
</author>
<author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yahata, M" uniqKey="Yahata M">M Yahata</name>
</author>
<author><name sortKey="Kunitake, H" uniqKey="Kunitake H">H Kunitake</name>
</author>
<author><name sortKey="Yabuya, T" uniqKey="Yabuya T">T Yabuya</name>
</author>
<author><name sortKey="Yamashita, K" uniqKey="Yamashita K">K Yamashita</name>
</author>
<author><name sortKey="Kashihara, Y" uniqKey="Kashihara Y">Y Kashihara</name>
</author>
<author><name sortKey="Komatsu, H" uniqKey="Komatsu H">H Komatsu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Esen, A" uniqKey="Esen A">A Esen</name>
</author>
<author><name sortKey="Soost, Rk" uniqKey="Soost R">RK Soost</name>
</author>
<author><name sortKey="Geraci, G" uniqKey="Geraci G">G Geraci</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="O Malley, Dm" uniqKey="O Malley D">DM O'Malley</name>
</author>
<author><name sortKey="Clevenger, D" uniqKey="Clevenger D">D Clevenger</name>
</author>
<author><name sortKey="Chaparro, Jx" uniqKey="Chaparro J">JX Chaparro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Juarez, J" uniqKey="Juarez J">J Juárez</name>
</author>
<author><name sortKey="Aleza, P" uniqKey="Aleza P">P Aleza</name>
</author>
<author><name sortKey="Pina, Ja" uniqKey="Pina J">JA Pina</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Maddy, F" uniqKey="Maddy F">F Maddy</name>
</author>
<author><name sortKey="Jacquemond, C" uniqKey="Jacquemond C">C Jacquemond</name>
</author>
<author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Rist, D" uniqKey="Rist D">D Rist</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Esen, A" uniqKey="Esen A">A Esen</name>
</author>
<author><name sortKey="Soost, Rk" uniqKey="Soost R">RK Soost</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Romero Aranda, R" uniqKey="Romero Aranda R">R Romero-Aranda</name>
</author>
<author><name sortKey="Bondada, Br" uniqKey="Bondada B">BR Bondada</name>
</author>
<author><name sortKey="Syvertsen, Jp" uniqKey="Syvertsen J">JP Syvertsen</name>
</author>
<author><name sortKey="Grosser, Jw" uniqKey="Grosser J">JW Grosser</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stupar, R" uniqKey="Stupar R">R Stupar</name>
</author>
<author><name sortKey="Bhaskar, Pb" uniqKey="Bhaskar P">PB Bhaskar</name>
</author>
<author><name sortKey="Yandell, Bs" uniqKey="Yandell B">BS Yandell</name>
</author>
<author><name sortKey="Rensik, Wa" uniqKey="Rensik W">WA Rensik</name>
</author>
<author><name sortKey="Hart, Al" uniqKey="Hart A">AL Hart</name>
</author>
<author><name sortKey="Ouyang, S" uniqKey="Ouyang S">S Ouyang</name>
</author>
<author><name sortKey="Veillux, Re" uniqKey="Veillux R">RE Veillux</name>
</author>
<author><name sortKey="Busse, Js" uniqKey="Busse J">JS Busse</name>
</author>
<author><name sortKey="Erhardt, Rj" uniqKey="Erhardt R">RJ Erhardt</name>
</author>
<author><name sortKey="Buell, Cr" uniqKey="Buell C">CR Buell</name>
</author>
<author><name sortKey="Jiang, J" uniqKey="Jiang J">J Jiang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yahata, M" uniqKey="Yahata M">M Yahata</name>
</author>
<author><name sortKey="Harusaki, S" uniqKey="Harusaki S">S Harusaki</name>
</author>
<author><name sortKey="Komatsu, H" uniqKey="Komatsu H">H Komatsu</name>
</author>
<author><name sortKey="Takami, K" uniqKey="Takami K">K Takami</name>
</author>
<author><name sortKey="Kunitake, H" uniqKey="Kunitake H">H Kunitake</name>
</author>
<author><name sortKey="Yabuya, T" uniqKey="Yabuya T">T Yabuya</name>
</author>
<author><name sortKey="Yamashita, K" uniqKey="Yamashita K">K Yamashita</name>
</author>
<author><name sortKey="Toolapong, P" uniqKey="Toolapong P">P Toolapong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pooler, M" uniqKey="Pooler M">M Pooler</name>
</author>
<author><name sortKey="Scorza, R" uniqKey="Scorza R">R Scorza</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barrett, Hc" uniqKey="Barrett H">HC Barrett</name>
</author>
<author><name sortKey="Hutchison, Dj" uniqKey="Hutchison D">DJ Hutchison</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Raghuvanshi, Ss" uniqKey="Raghuvanshi S">SS Raghuvanshi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yamamoto, M" uniqKey="Yamamoto M">M Yamamoto</name>
</author>
<author><name sortKey="Tominaga, S" uniqKey="Tominaga S">S Tominaga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Juarez, J" uniqKey="Juarez J">J Juárez</name>
</author>
<author><name sortKey="Aleza, P" uniqKey="Aleza P">P Aleza</name>
</author>
<author><name sortKey="Olivares Fuster, O" uniqKey="Olivares Fuster O">O Olivares-Fuster</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murashige, T" uniqKey="Murashige T">T Murashige</name>
</author>
<author><name sortKey="Skoog, F" uniqKey="Skoog F">F Skoog</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dellaporta, J" uniqKey="Dellaporta J">J Dellaporta</name>
</author>
<author><name sortKey="Hicks, Jb" uniqKey="Hicks J">JB Hicks</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kijas, Jmh" uniqKey="Kijas J">JMH Kijas</name>
</author>
<author><name sortKey="Thomas, Mr" uniqKey="Thomas M">MR Thomas</name>
</author>
<author><name sortKey="Fowler, Jcs" uniqKey="Fowler J">JCS Fowler</name>
</author>
<author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Dambier, D" uniqKey="Dambier D">D Dambier</name>
</author>
<author><name sortKey="Bassene, Jb" uniqKey="Bassene J">JB Bassene</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Lotfy, S" uniqKey="Lotfy S">S Lotfy</name>
</author>
<author><name sortKey="Didout, C" uniqKey="Didout C">C Didout</name>
</author>
<author><name sortKey="Beaumont, V" uniqKey="Beaumont V">V Beaumont</name>
</author>
<author><name sortKey="Brottier, P" uniqKey="Brottier P">P Brottier</name>
</author>
<author><name sortKey="Risterucci, Am" uniqKey="Risterucci A">AM Risterucci</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Benbouzas, H" uniqKey="Benbouzas H">H Benbouzas</name>
</author>
<author><name sortKey="Jacquemin, Jm" uniqKey="Jacquemin J">JM Jacquemin</name>
</author>
<author><name sortKey="Baudoin, Jp" uniqKey="Baudoin J">JP Baudoin</name>
</author>
<author><name sortKey="Mergeai, G" uniqKey="Mergeai G">G Mergeai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="D Hont, A" uniqKey="D Hont A">A D'Hont</name>
</author>
<author><name sortKey="Grivet, L" uniqKey="Grivet L">L Grivet</name>
</author>
<author><name sortKey="Feldmann, P" uniqKey="Feldmann P">P Feldmann</name>
</author>
<author><name sortKey="Rao, P" uniqKey="Rao P">P Rao</name>
</author>
<author><name sortKey="Berding, N" uniqKey="Berding N">N Berding</name>
</author>
<author><name sortKey="Glaszmann, Jc" uniqKey="Glaszmann J">JC Glaszmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jensen, Wa" uniqKey="Jensen W">WA Jensen</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article" xml:lang="en"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Plant Biol</journal-id>
<journal-id journal-id-type="iso-abbrev">BMC Plant Biol</journal-id>
<journal-title-group><journal-title>BMC Plant Biology</journal-title>
</journal-title-group>
<issn pub-type="epub">1471-2229</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">19698121</article-id>
<article-id pub-id-type="pmc">2747335</article-id>
<article-id pub-id-type="publisher-id">1471-2229-9-110</article-id>
<article-id pub-id-type="doi">10.1186/1471-2229-9-110</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Recovery and characterization of a <italic>Citrus clementina </italic>
Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" id="A1"><name><surname>Aleza</surname>
<given-names>Pablo</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>aleza@ivia.es</email>
</contrib>
<contrib contrib-type="author" id="A2"><name><surname>Juárez</surname>
<given-names>José</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>jjuarez@ivia.es</email>
</contrib>
<contrib contrib-type="author" id="A3"><name><surname>Hernández</surname>
<given-names>María</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>mariaher@ivia.es</email>
</contrib>
<contrib contrib-type="author" id="A4"><name><surname>Pina</surname>
<given-names>José A</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>japina@ivia.es</email>
</contrib>
<contrib contrib-type="author" id="A5"><name><surname>Ollitrault</surname>
<given-names>Patrick</given-names>
</name>
<xref ref-type="aff" rid="I2">2</xref>
<email>patrick.ollitrault@cirad.fr</email>
</contrib>
<contrib contrib-type="author" corresp="yes" id="A6"><name><surname>Navarro</surname>
<given-names>Luis</given-names>
</name>
<xref ref-type="aff" rid="I1">1</xref>
<email>lnavarro@ivia.es</email>
</contrib>
</contrib-group>
<aff id="I1"><label>1</label>
Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Ctra. Moncada-Náquera km 4.5, 46113 Moncada, Valencia, Spain</aff>
<aff id="I2"><label>2</label>
Unité de Recherche Multiplication Végétative, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Montpellier 34398, France</aff>
<pub-date pub-type="collection"><year>2009</year>
</pub-date>
<pub-date pub-type="epub"><day>22</day>
<month>8</month>
<year>2009</year>
</pub-date>
<volume>9</volume>
<fpage>110</fpage>
<lpage>110</lpage>
<history><date date-type="received"><day>16</day>
<month>2</month>
<year>2009</year>
</date>
<date date-type="accepted"><day>22</day>
<month>8</month>
<year>2009</year>
</date>
</history>
<permissions><copyright-statement>Copyright ©2009 Aleza et al; licensee BioMed Central Ltd.</copyright-statement>
<copyright-year>2009</copyright-year>
<copyright-holder>Aleza et al; licensee BioMed Central Ltd.</copyright-holder>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<self-uri xlink:href="http://www.biomedcentral.com/1471-2229/9/110"></self-uri>
<abstract><sec><title>Background</title>
<p>In recent years, the development of structural genomics has generated a growing interest in obtaining haploid plants. The use of homozygous lines presents a significant advantage for the accomplishment of sequencing projects. Commercial citrus species are characterized by high heterozygosity, making it difficult to assemble large genome sequences. Thus, the International Citrus Genomic Consortium (ICGC) decided to establish a reference whole citrus genome sequence from a homozygous plant. Due to the existence of important molecular resources and previous success in obtaining haploid clementine plants, haploid clementine was selected as the target for the implementation of the reference whole genome citrus sequence.</p>
</sec>
<sec><title>Results</title>
<p>To obtain haploid clementine lines we used the technique of <italic>in situ </italic>
gynogenesis induced by irradiated pollen. Flow cytometry, chromosome counts and SSR marker (Simple Sequence Repeats) analysis facilitated the identification of six different haploid lines (2<italic>n </italic>
= <italic>x </italic>
= 9), one aneuploid line (2<italic>n </italic>
= 2<italic>x</italic>
+4 = 22) and one doubled haploid plant (2<italic>n </italic>
= 2<italic>x </italic>
= 18) of 'Clemenules' clementine. One of the haploids, obtained directly from an original haploid embryo, grew vigorously and produced flowers after four years. This is the first haploid plant of clementine that has bloomed and we have, for the first time, characterized the histology of haploid and diploid flowers of clementine. Additionally a double haploid plant was obtained spontaneously from this haploid line.</p>
</sec>
<sec><title>Conclusion</title>
<p>The first haploid plant of 'Clemenules' clementine produced directly by germination of a haploid embryo, which grew vigorously and produced flowers, has been obtained in this work. This haploid line has been selected and it is being used by the ICGC to establish the reference sequence of the nuclear genome of citrus.</p>
</sec>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Espagne</li>
<li>France</li>
</country>
<region><li>Languedoc-Roussillon</li>
<li>Occitanie (région administrative)</li>
</region>
</list>
<tree><country name="Espagne"><noRegion><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name>
</noRegion>
<name sortKey="Hernandez, Maria" sort="Hernandez, Maria" uniqKey="Hernandez M" first="María" last="Hernández">María Hernández</name>
<name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name>
<name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name>
<name sortKey="Pina, Jose A" sort="Pina, Jose A" uniqKey="Pina J" first="José A" last="Pina">José A. Pina</name>
</country>
<country name="France"><region name="Occitanie (région administrative)"><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001000 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 001000 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Pmc |étape= Checkpoint |type= RBID |clé= PMC:2747335 |texte= Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome sequence }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:19698121" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
![]() | This area was generated with Dilib version V0.6.25. | ![]() |