An endogenous F-box protein regulates ARGONAUTE1 in Arabidopsis thaliana
Identifieur interne : 000F76 ( Pmc/Checkpoint ); précédent : 000F75; suivant : 000F77An endogenous F-box protein regulates ARGONAUTE1 in Arabidopsis thaliana
Auteurs : K. Earley [États-Unis] ; Mr Smith [États-Unis] ; R. Weber [États-Unis] ; Bd Gregory [États-Unis] ; Rs Poethig [États-Unis]Source :
- Silence [ 1758-907X ] ; 2010.
Abstract
ARGONAUTE1 (AGO1) mediates microRNA- and small interfering RNA-directed posttranscriptional gene silencing in
Url:
DOI: 10.1186/1758-907X-1-15
PubMed: 20624295
PubMed Central: 2914764
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An endogenous F-box protein regulates ARGONAUTE1 in <italic>Arabidopsis thaliana</italic></title><author><name sortKey="Earley, K" sort="Earley, K" uniqKey="Earley K" first="K" last="Earley">K. Earley</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Smith, Mr" sort="Smith, Mr" uniqKey="Smith M" first="Mr" last="Smith">Mr Smith</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Weber, R" sort="Weber, R" uniqKey="Weber R" first="R" last="Weber">R. Weber</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Gregory, Bd" sort="Gregory, Bd" uniqKey="Gregory B" first="Bd" last="Gregory">Bd Gregory</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Poethig, Rs" sort="Poethig, Rs" uniqKey="Poethig R" first="Rs" last="Poethig">Rs Poethig</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20624295</idno><idno type="pmc">2914764</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914764</idno><idno type="RBID">PMC:2914764</idno><idno type="doi">10.1186/1758-907X-1-15</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">001297</idno><idno type="wicri:Area/Pmc/Curation">001295</idno><idno type="wicri:Area/Pmc/Checkpoint">000F76</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An endogenous F-box protein regulates ARGONAUTE1 in <italic>Arabidopsis thaliana</italic></title><author><name sortKey="Earley, K" sort="Earley, K" uniqKey="Earley K" first="K" last="Earley">K. Earley</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Smith, Mr" sort="Smith, Mr" uniqKey="Smith M" first="Mr" last="Smith">Mr Smith</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Weber, R" sort="Weber, R" uniqKey="Weber R" first="R" last="Weber">R. Weber</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Gregory, Bd" sort="Gregory, Bd" uniqKey="Gregory B" first="Bd" last="Gregory">Bd Gregory</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author><author><name sortKey="Poethig, Rs" sort="Poethig, Rs" uniqKey="Poethig R" first="Rs" last="Poethig">Rs Poethig</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, University of Pennsylvania, Philadelphia PA 19104</wicri:regionArea><wicri:noRegion>Philadelphia PA 19104</wicri:noRegion></affiliation></author></analytic><series><title level="j">Silence</title><idno type="eISSN">1758-907X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>ARGONAUTE1 (AGO1) mediates microRNA- and small interfering RNA-directed posttranscriptional gene silencing in <italic>Arabidopsis thaliana</italic>. Mutant alleles of <italic>SQUINT </italic>(<italic>SQN</italic>) slightly reduce AGO1 activity and have weak effects on shoot morphology. A screen for mutations that suppress the <italic>sqn </italic>phenotype produced loss-of-function mutations in the F-box gene <italic>FBW2</italic>. Mutations in <italic>FBW2 </italic>not only suppress <italic>sqn </italic>but also suppress many of the developmental phenotypes of weak, but not null, alleles of <italic>AGO1 </italic>by increasing AGO1 protein levels. Conversely, over-expression of FBW2 decreases the abundance of the AGO1 protein but not <italic>AGO1 </italic>messenger RNA, further indicating that FBW2 regulates AGO1 protein levels. <italic>fbw2 </italic>mutants have no obvious morphological phenotype, but display a reduced sensitivity to abscisic acid (ABA) that can be attributed to increased AGO1 activity. 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Clough</name></author><author><name sortKey="Bent, Af" uniqKey="Bent A">AF Bent</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Silence</journal-id><journal-title-group><journal-title>Silence</journal-title></journal-title-group><issn pub-type="epub">1758-907X</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20624295</article-id><article-id pub-id-type="pmc">2914764</article-id><article-id pub-id-type="publisher-id">1758-907X-1-15</article-id><article-id pub-id-type="doi">10.1186/1758-907X-1-15</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research </subject></subj-group></article-categories><title-group><article-title>An endogenous F-box protein regulates ARGONAUTE1 in <italic>Arabidopsis thaliana</italic></article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Earley</surname><given-names>K</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>kwearley@gmail.com</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Smith</surname><given-names>MR</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>mrsmith4@gmail.com</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Weber</surname><given-names>R</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>raw623@gmail.com</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Gregory</surname><given-names>BD</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>bdgregor@sas.upenn.edu</email></contrib><contrib contrib-type="author" corresp="yes" id="A5"><name><surname>Poethig</surname><given-names>RS</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>spoethig@sas.upenn.edu</email></contrib></contrib-group><aff id="I1"><label>1</label>Department of Biology, University of Pennsylvania, Philadelphia PA 19104, USA</aff><pub-date pub-type="collection"><year>2010</year></pub-date><pub-date pub-type="epub"><day>12</day><month>7</month><year>2010</year></pub-date><volume>1</volume><fpage>15</fpage><lpage>15</lpage><history><date date-type="received"><day>23</day><month>3</month><year>2010</year></date><date date-type="accepted"><day>12</day><month>7</month><year>2010</year></date></history><permissions><copyright-statement>Copyright ©2010 Earley et al; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2010</copyright-year><copyright-holder>Earley et al; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.silencejournal.com/content/1/1/15"></self-uri><abstract><p>ARGONAUTE1 (AGO1) mediates microRNA- and small interfering RNA-directed posttranscriptional gene silencing in <italic>Arabidopsis thaliana</italic>. Mutant alleles of <italic>SQUINT </italic>(<italic>SQN</italic>) slightly reduce AGO1 activity and have weak effects on shoot morphology. A screen for mutations that suppress the <italic>sqn </italic>phenotype produced loss-of-function mutations in the F-box gene <italic>FBW2</italic>. Mutations in <italic>FBW2 </italic>not only suppress <italic>sqn </italic>but also suppress many of the developmental phenotypes of weak, but not null, alleles of <italic>AGO1 </italic>by increasing AGO1 protein levels. Conversely, over-expression of FBW2 decreases the abundance of the AGO1 protein but not <italic>AGO1 </italic>messenger RNA, further indicating that FBW2 regulates AGO1 protein levels. <italic>fbw2 </italic>mutants have no obvious morphological phenotype, but display a reduced sensitivity to abscisic acid (ABA) that can be attributed to increased AGO1 activity. Our results indicate that FBW2 is a novel negative regulator of AGO1 and suggest that it plays a role in ABA signalling and/or response.</p></abstract></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Earley, K" sort="Earley, K" uniqKey="Earley K" first="K" last="Earley">K. Earley</name></noRegion><name sortKey="Gregory, Bd" sort="Gregory, Bd" uniqKey="Gregory B" first="Bd" last="Gregory">Bd Gregory</name><name sortKey="Poethig, Rs" sort="Poethig, Rs" uniqKey="Poethig R" first="Rs" last="Poethig">Rs Poethig</name><name sortKey="Smith, Mr" sort="Smith, Mr" uniqKey="Smith M" first="Mr" last="Smith">Mr Smith</name><name sortKey="Weber, R" sort="Weber, R" uniqKey="Weber R" first="R" last="Weber">R. Weber</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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