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Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches

Identifieur interne : 000B13 ( Pmc/Checkpoint ); précédent : 000B12; suivant : 000B14

Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches

Auteurs : Qiaoli Ma [République populaire de Chine] ; Yuduan Ding [République populaire de Chine] ; Jiwei Chang [République populaire de Chine] ; Xiaohua Sun [République populaire de Chine] ; Li Zhang [République populaire de Chine] ; Qingjiang Wei [République populaire de Chine] ; Yunjiang Cheng [République populaire de Chine] ; Lingling Chen [République populaire de Chine] ; Juan Xu [République populaire de Chine] ; Xiuxin Deng [République populaire de Chine]

Source :

RBID : PMC:3883282

Abstract

2,4-D retards senescence of postharvest citrus fruits by increasing exogenous auxin, endogenous ABA and SA contents, while decreasing ethylene production; and enhancing stress-defense capability through changing epicuticular wax morphology and lignin content in peel


Url:
DOI: 10.1093/jxb/ert344
PubMed: 24215076
PubMed Central: 3883282


Affiliations:


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PMC:3883282

Le document en format XML

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<p>2,4-D retards senescence of postharvest citrus fruits by increasing exogenous auxin, endogenous ABA and SA contents, while decreasing ethylene production; and enhancing stress-defense capability through changing epicuticular wax morphology and lignin content in peel</p>
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<pmc-dir>properties open_access</pmc-dir>
<front>
<journal-meta>
<journal-id journal-id-type="nlm-ta">J Exp Bot</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Exp. Bot</journal-id>
<journal-id journal-id-type="hwp">jexbot</journal-id>
<journal-id journal-id-type="publisher-id">jexbot</journal-id>
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<journal-title>Journal of Experimental Botany</journal-title>
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<issn pub-type="ppub">0022-0957</issn>
<issn pub-type="epub">1460-2431</issn>
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<publisher-name>Oxford University Press</publisher-name>
<publisher-loc>UK</publisher-loc>
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<article-meta>
<article-id pub-id-type="pmid">24215076</article-id>
<article-id pub-id-type="pmc">3883282</article-id>
<article-id pub-id-type="doi">10.1093/jxb/ert344</article-id>
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<subject>Research Paper</subject>
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<title-group>
<article-title>Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches</article-title>
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<xref ref-type="aff" rid="AF0001">
<sup>1</sup>
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<sup>1</sup>
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<xref ref-type="aff" rid="AF0002">
<sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no">
<name>
<surname>Sun</surname>
<given-names>Xiaohua</given-names>
</name>
<xref ref-type="aff" rid="AF0001">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no">
<name>
<surname>Zhang</surname>
<given-names>Li</given-names>
</name>
<xref ref-type="aff" rid="AF0001">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no">
<name>
<surname>Wei</surname>
<given-names>Qingjiang</given-names>
</name>
<xref ref-type="aff" rid="AF0001">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Cheng</surname>
<given-names>Yunjiang</given-names>
</name>
<xref ref-type="aff" rid="AF0001">
<sup>1</sup>
</xref>
<xref ref-type="corresp" rid="c1">*</xref>
</contrib>
<contrib contrib-type="author" corresp="no">
<name>
<surname>Chen</surname>
<given-names>Lingling</given-names>
</name>
<xref ref-type="aff" rid="AF0002">
<sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no">
<name>
<surname>Xu</surname>
<given-names>Juan</given-names>
</name>
<xref ref-type="aff" rid="AF0001">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="no">
<name>
<surname>Deng</surname>
<given-names>Xiuxin</given-names>
</name>
<xref ref-type="aff" rid="AF0001">
<sup>1</sup>
</xref>
</contrib>
<aff id="AF0001">
<sup>1</sup>
<institution>Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University</institution>
,
<addr-line>Wuhan 430070</addr-line>
,
<country>PR China</country>
</aff>
<aff id="AF0002">
<sup>2</sup>
<institution>Center for Bioinformatics, College of Life Science and Technology, Huazhong Agricultural University</institution>
,
<addr-line>Wuhan 430070</addr-line>
,
<country>PR China</country>
</aff>
</contrib-group>
<author-notes>
<corresp id="c1">* To whom correspondence should be addressed. E-mail:
<email>yjcheng@mail.hzau.edu.cn</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub">
<month>1</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="epub">
<day>8</day>
<month>11</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release">
<day>8</day>
<month>11</month>
<year>2013</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the . </pmc-comment>
<volume>65</volume>
<issue>1</issue>
<fpage>61</fpage>
<lpage>74</lpage>
<permissions>
<copyright-statement>© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.</copyright-statement>
<copyright-year>2013</copyright-year>
<license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by/3.0">
<license-p>
<pmc-comment>CREATIVE COMMONS</pmc-comment>
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">http://creativecommons.org/licenses/by/3.0/</ext-link>
), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract abstract-type="precis">
<p>2,4-D retards senescence of postharvest citrus fruits by increasing exogenous auxin, endogenous ABA and SA contents, while decreasing ethylene production; and enhancing stress-defense capability through changing epicuticular wax morphology and lignin content in peel</p>
</abstract>
<abstract>
<p>Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. However, no other substitutes for 2,4-D are available to the post-harvest industry. Insights into the molecular mechanism underlying the effects of 2,4-D on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. This study comprehensively analysed changes in the peel of Olinda Valencia orange [
<italic>Citrus sinensis</italic>
(L.) Osbeck] induced by 500 ppm 2,4-D using ‘omic’-driven approaches. Transcriptional profiling revealed that transcriptional factor (mainly AP2/ERF, WRKY, and NAC family members), transport, and hormone metabolism genes were over-represented and up-regulated within 24h post-treatment (HPT). Stress defence genes were up-regulated, while cell wall metabolism genes were down-regulated after 48 HPT. However, secondary metabolism genes, especially phenylpropanoid and lignin biosynthesis-related genes, were over-represented at all the time points. Comparative proteomic analysis indicated that the expression of proteins implicated in stress responses (25%), hormone metabolism, and signal transduction (12%) significantly accumulated at the post-transcriptional level. Hormone levels detected by high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) showed that abscisic acid, salicylic acid, and 2,4-D significantly increased, while ethylene production (detected by gas chromatography) decreased after 2,4-D treatment. In addition, lignin and water content in the fruit peel also increased and the epicuticle wax ultrastructure was modified. In conclusion, 2,4-D retarded fruit senescence by altering the levels of many endogenous hormones and by improving stress defence capabilities by up-regulating defence-related genes and proteins.</p>
</abstract>
<kwd-group>
<title>Key words:</title>
<kwd>ABA</kwd>
<kwd>auxin</kwd>
<kwd>citrus fruit</kwd>
<kwd>2,4-dichlorophenoxy acetic acid (2,4-D)</kwd>
<kwd>ethylene</kwd>
<kwd>fruit quality preservation</kwd>
<kwd>fruit senescence</kwd>
<kwd>plant hormones</kwd>
<kwd>stress defence.</kwd>
</kwd-group>
<counts>
<page-count count="14"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations>
<list>
<country>
<li>République populaire de Chine</li>
</country>
</list>
<tree>
<country name="République populaire de Chine">
<noRegion>
<name sortKey="Ma, Qiaoli" sort="Ma, Qiaoli" uniqKey="Ma Q" first="Qiaoli" last="Ma">Qiaoli Ma</name>
</noRegion>
<name sortKey="Chang, Jiwei" sort="Chang, Jiwei" uniqKey="Chang J" first="Jiwei" last="Chang">Jiwei Chang</name>
<name sortKey="Chen, Lingling" sort="Chen, Lingling" uniqKey="Chen L" first="Lingling" last="Chen">Lingling Chen</name>
<name sortKey="Cheng, Yunjiang" sort="Cheng, Yunjiang" uniqKey="Cheng Y" first="Yunjiang" last="Cheng">Yunjiang Cheng</name>
<name sortKey="Deng, Xiuxin" sort="Deng, Xiuxin" uniqKey="Deng X" first="Xiuxin" last="Deng">Xiuxin Deng</name>
<name sortKey="Ding, Yuduan" sort="Ding, Yuduan" uniqKey="Ding Y" first="Yuduan" last="Ding">Yuduan Ding</name>
<name sortKey="Sun, Xiaohua" sort="Sun, Xiaohua" uniqKey="Sun X" first="Xiaohua" last="Sun">Xiaohua Sun</name>
<name sortKey="Wei, Qingjiang" sort="Wei, Qingjiang" uniqKey="Wei Q" first="Qingjiang" last="Wei">Qingjiang Wei</name>
<name sortKey="Xu, Juan" sort="Xu, Juan" uniqKey="Xu J" first="Juan" last="Xu">Juan Xu</name>
<name sortKey="Zhang, Li" sort="Zhang, Li" uniqKey="Zhang L" first="Li" last="Zhang">Li Zhang</name>
</country>
</tree>
</affiliations>
</record>

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