Pectin Methylesterase and Pectin Remodelling Differ in the Fibre Walls of Two Gossypium Species with Very Different Fibre Properties
Identifieur interne : 000A11 ( Pmc/Checkpoint ); précédent : 000A10; suivant : 000A12Pectin Methylesterase and Pectin Remodelling Differ in the Fibre Walls of Two Gossypium Species with Very Different Fibre Properties
Auteurs : Qinxiang Liu ; Mark Talbot ; Danny J. LlewellynSource :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Pectin, a major component of the primary cell walls of dicot plants, is synthesized in Golgi, secreted into the wall as methylesters and subsequently de-esterified by pectin methylesterase (PME). Pectin remodelling by PMEs is known to be important in regulating cell expansion in plants, but has been poorly studied in cotton. In this study, genome-wide analysis showed that
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DOI: 10.1371/journal.pone.0065131
PubMed: 23755181
PubMed Central: 3673955
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pectin Methylesterase and Pectin Remodelling Differ in the Fibre Walls of Two <italic>Gossypium</italic> Species with Very Different Fibre Properties</title><author><name sortKey="Liu, Qinxiang" sort="Liu, Qinxiang" uniqKey="Liu Q" first="Qinxiang" last="Liu">Qinxiang Liu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Talbot, Mark" sort="Talbot, Mark" uniqKey="Talbot M" first="Mark" last="Talbot">Mark Talbot</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Llewellyn, Danny J" sort="Llewellyn, Danny J" uniqKey="Llewellyn D" first="Danny J." last="Llewellyn">Danny J. Llewellyn</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23755181</idno><idno type="pmc">3673955</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3673955</idno><idno type="RBID">PMC:3673955</idno><idno type="doi">10.1371/journal.pone.0065131</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">001144</idno><idno type="wicri:Area/Pmc/Curation">001143</idno><idno type="wicri:Area/Pmc/Checkpoint">000A11</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Pectin Methylesterase and Pectin Remodelling Differ in the Fibre Walls of Two <italic>Gossypium</italic> Species with Very Different Fibre Properties</title><author><name sortKey="Liu, Qinxiang" sort="Liu, Qinxiang" uniqKey="Liu Q" first="Qinxiang" last="Liu">Qinxiang Liu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Talbot, Mark" sort="Talbot, Mark" uniqKey="Talbot M" first="Mark" last="Talbot">Mark Talbot</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Llewellyn, Danny J" sort="Llewellyn, Danny J" uniqKey="Llewellyn D" first="Danny J." last="Llewellyn">Danny J. Llewellyn</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Pectin, a major component of the primary cell walls of dicot plants, is synthesized in Golgi, secreted into the wall as methylesters and subsequently de-esterified by pectin methylesterase (PME). Pectin remodelling by PMEs is known to be important in regulating cell expansion in plants, but has been poorly studied in cotton. In this study, genome-wide analysis showed that <italic>PME</italic>s are a large multi-gene family (81 genes) in diploid cotton (<italic>Gossypium raimondii</italic>), an expansion over the 66 in <italic>Arabidopsis</italic> and suggests the evolution of new functions in cotton. Relatively few <italic>PME</italic> genes are expressed highly in fibres based on EST abundance and the five most abundant in fibres were cloned and sequenced from two cotton species. Their significant sequence differences and their stage-specific expression in fibres within a species suggest sub-specialisation during fibre development. We determined the transcript abundance of the five fibre <italic>PMEs</italic>, total PME enzyme activity, pectin content and extent of de-methylesterification of the pectin in fibre walls of the two cotton species over the first 25–30 days of fibre growth. There was a higher transcript abundance of fibre-<italic>PME</italic>s and a higher total PME enzyme activity in <italic>G. barbadense (Gb)</italic> than in <italic>G. hirsutum (Gh)</italic> fibres, particularly during late fibre elongation. Total pectin was high, but de-esterified pectin was low during fibre elongation (5–12 dpa) in both <italic>Gh</italic> and <italic>Gb</italic>. De-esterified pectin levels rose thereafter when total PME activity increased and this occurred earlier in <italic>Gb</italic> fibres resulting in a lower degree of esterification in <italic>Gb</italic> fibres between 17 and 22 dpa. <italic>Gb</italic> fibres are finer and longer than those of <italic>Gh</italic>, so differences in pectin remodelling during the transition to wall thickening may be an important factor in influencing final fibre diameter and length, two key quality attributes of cotton fibres.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cosgrove, D" uniqKey="Cosgrove D">D Cosgrove</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Willats, W" uniqKey="Willats W">W Willats</name></author><author><name sortKey="Mccartney, L" uniqKey="Mccartney L">L McCartney</name></author><author><name sortKey="Mackie, W" uniqKey="Mackie W">W Mackie</name></author><author><name sortKey="Knox, J" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23755181</article-id><article-id pub-id-type="pmc">3673955</article-id><article-id pub-id-type="publisher-id">PONE-D-13-04485</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0065131</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Agriculture</subject><subj-group><subject>Crops</subject><subj-group><subject>Fibers</subject><subj-group><subject>Cotton</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Enzymes</subject><subj-group><subject>Enzyme Classes</subject><subj-group><subject>Esterases</subject></subj-group></subj-group></subj-group><subj-group><subject>Plant Biochemistry</subject><subj-group><subject>Carbohydrate Biosynthesis</subject></subj-group></subj-group></subj-group><subj-group><subject>Computational Biology</subject><subj-group><subject>Genomics</subject><subj-group><subject>Genome Analysis Tools</subject><subj-group><subject>Genome-Wide Association Studies</subject></subj-group></subj-group><subj-group><subject>Genome Expression Analysis</subject><subject>Genome Sequencing</subject></subj-group></subj-group><subj-group><subject>Molecular Genetics</subject><subj-group><subject>Gene Expression</subject></subj-group></subj-group></subj-group><subj-group><subject>Developmental Biology</subject><subj-group><subject>Plant Growth and Development</subject></subj-group></subj-group><subj-group><subject>Molecular Cell Biology</subject><subj-group><subject>Plant Cell Biology</subject><subj-group><subject>Plant Cell Wall</subject></subj-group></subj-group></subj-group><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Cell Biology</subject><subj-group><subject>Plant Cell Wall</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Pectin Methylesterase and Pectin Remodelling Differ in the Fibre Walls of Two <italic>Gossypium</italic> Species with Very Different Fibre Properties</article-title><alt-title alt-title-type="running-head">Pectin Remodelling in Cotton Fibres</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Qinxiang</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Talbot</surname><given-names>Mark</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Llewellyn</surname><given-names>Danny J.</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><addr-line>Plant Industry, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra, Australian Capital Territory, Australia</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Heazlewood</surname><given-names>Joshua L.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Lawrence Berkeley National Laboratory, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>Danny.Llewellyn@csiro.au</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: DL QL. Performed the experiments: QL MT. Analyzed the data: DL QL MT. Contributed reagents/materials/analysis tools: DL QL MT. Wrote the paper: QL DL.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>5</day><month>6</month><year>2013</year></pub-date><volume>8</volume><issue>6</issue><elocation-id>e65131</elocation-id><history><date date-type="received"><day>30</day><month>1</month><year>2013</year></date><date date-type="accepted"><day>22</day><month>4</month><year>2013</year></date></history><permissions><copyright-year>2013</copyright-year><copyright-holder>Liu et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><p>Pectin, a major component of the primary cell walls of dicot plants, is synthesized in Golgi, secreted into the wall as methylesters and subsequently de-esterified by pectin methylesterase (PME). Pectin remodelling by PMEs is known to be important in regulating cell expansion in plants, but has been poorly studied in cotton. In this study, genome-wide analysis showed that <italic>PME</italic>s are a large multi-gene family (81 genes) in diploid cotton (<italic>Gossypium raimondii</italic>), an expansion over the 66 in <italic>Arabidopsis</italic> and suggests the evolution of new functions in cotton. Relatively few <italic>PME</italic> genes are expressed highly in fibres based on EST abundance and the five most abundant in fibres were cloned and sequenced from two cotton species. Their significant sequence differences and their stage-specific expression in fibres within a species suggest sub-specialisation during fibre development. We determined the transcript abundance of the five fibre <italic>PMEs</italic>, total PME enzyme activity, pectin content and extent of de-methylesterification of the pectin in fibre walls of the two cotton species over the first 25–30 days of fibre growth. There was a higher transcript abundance of fibre-<italic>PME</italic>s and a higher total PME enzyme activity in <italic>G. barbadense (Gb)</italic> than in <italic>G. hirsutum (Gh)</italic> fibres, particularly during late fibre elongation. Total pectin was high, but de-esterified pectin was low during fibre elongation (5–12 dpa) in both <italic>Gh</italic> and <italic>Gb</italic>. De-esterified pectin levels rose thereafter when total PME activity increased and this occurred earlier in <italic>Gb</italic> fibres resulting in a lower degree of esterification in <italic>Gb</italic> fibres between 17 and 22 dpa. <italic>Gb</italic> fibres are finer and longer than those of <italic>Gh</italic>, so differences in pectin remodelling during the transition to wall thickening may be an important factor in influencing final fibre diameter and length, two key quality attributes of cotton fibres.</p></abstract><funding-group><funding-statement>This work was supported by Cotton Breeding Australia, a joint venture between the Australian Government research organisation CSIRO and Cotton Seed Distributors a grower owned and controlled co-operative of Australian cotton farmers. All authors are employees of the Australian government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="13"></page-count></counts></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Liu, Qinxiang" sort="Liu, Qinxiang" uniqKey="Liu Q" first="Qinxiang" last="Liu">Qinxiang Liu</name><name sortKey="Llewellyn, Danny J" sort="Llewellyn, Danny J" uniqKey="Llewellyn D" first="Danny J." last="Llewellyn">Danny J. Llewellyn</name><name sortKey="Talbot, Mark" sort="Talbot, Mark" uniqKey="Talbot M" first="Mark" last="Talbot">Mark Talbot</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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