Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis (L.) Osbeck
Identifieur interne : 000886 ( Pmc/Checkpoint ); précédent : 000885; suivant : 000887Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis (L.) Osbeck
Auteurs : Concetta Licciardello [Italie] ; Nunzio D Gostino [Italie] ; Alessandra Traini [Royaume-Uni] ; Giuseppe Reforgiato Recupero [Italie] ; Luigi Frusciante [Italie] ; Maria Luisa Chiusano [Italie]Source :
- BMC Plant Biology [ 1471-2229 ] ; 2014.
Abstract
Glutathione S-transferases (GSTs) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. Anthocyanin pigments require GSTs for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. Anthocyanin accumulation in
We identified 61 GST transcripts, described the full- or partial-length nature of the sequences and assigned to each sequence the GST class membership exploiting a comparative approach and the classification scheme proposed for plant species
This study aimed at the characterization of the GST gene family in
Url:
DOI: 10.1186/1471-2229-14-39
PubMed: 24490620
PubMed Central: 3922800
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of <italic>Citrus sinensis</italic> (L.) Osbeck</title><author><name sortKey="Licciardello, Concetta" sort="Licciardello, Concetta" uniqKey="Licciardello C" first="Concetta" last="Licciardello">Concetta Licciardello</name><affiliation wicri:level="1"><nlm:aff id="I1">Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania</wicri:regionArea><wicri:noRegion>Catania</wicri:noRegion></affiliation></author><author><name sortKey="D Gostino, Nunzio" sort="D Gostino, Nunzio" uniqKey="D Gostino N" first="Nunzio" last="D Gostino">Nunzio D Gostino</name><affiliation wicri:level="1"><nlm:aff id="I2">Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Orticoltura (CRA-ORT), via Cavalleggeri 25, 84098 Pontecagnano, Salerno, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Orticoltura (CRA-ORT), via Cavalleggeri 25, 84098 Pontecagnano, Salerno</wicri:regionArea><wicri:noRegion>Salerno</wicri:noRegion></affiliation></author><author><name sortKey="Traini, Alessandra" sort="Traini, Alessandra" uniqKey="Traini A" first="Alessandra" last="Traini">Alessandra Traini</name><affiliation wicri:level="1"><nlm:aff id="I4">Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA</wicri:regionArea><wicri:noRegion>Cambridgeshire CB10 1SA</wicri:noRegion></affiliation></author><author><name sortKey="Recupero, Giuseppe Reforgiato" sort="Recupero, Giuseppe Reforgiato" uniqKey="Recupero G" first="Giuseppe Reforgiato" last="Recupero">Giuseppe Reforgiato Recupero</name><affiliation wicri:level="1"><nlm:aff id="I1">Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania</wicri:regionArea><wicri:noRegion>Catania</wicri:noRegion></affiliation></author><author><name sortKey="Frusciante, Luigi" sort="Frusciante, Luigi" uniqKey="Frusciante L" first="Luigi" last="Frusciante">Luigi Frusciante</name><affiliation wicri:level="1"><nlm:aff id="I3">Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Chiusano, Maria Luisa" sort="Chiusano, Maria Luisa" uniqKey="Chiusano M" first="Maria Luisa" last="Chiusano">Maria Luisa Chiusano</name><affiliation wicri:level="1"><nlm:aff id="I3">Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24490620</idno><idno type="pmc">3922800</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922800</idno><idno type="RBID">PMC:3922800</idno><idno type="doi">10.1186/1471-2229-14-39</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000B47</idno><idno type="wicri:Area/Pmc/Curation">000B46</idno><idno type="wicri:Area/Pmc/Checkpoint">000886</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of <italic>Citrus sinensis</italic> (L.) Osbeck</title><author><name sortKey="Licciardello, Concetta" sort="Licciardello, Concetta" uniqKey="Licciardello C" first="Concetta" last="Licciardello">Concetta Licciardello</name><affiliation wicri:level="1"><nlm:aff id="I1">Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania</wicri:regionArea><wicri:noRegion>Catania</wicri:noRegion></affiliation></author><author><name sortKey="D Gostino, Nunzio" sort="D Gostino, Nunzio" uniqKey="D Gostino N" first="Nunzio" last="D Gostino">Nunzio D Gostino</name><affiliation wicri:level="1"><nlm:aff id="I2">Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Orticoltura (CRA-ORT), via Cavalleggeri 25, 84098 Pontecagnano, Salerno, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Orticoltura (CRA-ORT), via Cavalleggeri 25, 84098 Pontecagnano, Salerno</wicri:regionArea><wicri:noRegion>Salerno</wicri:noRegion></affiliation></author><author><name sortKey="Traini, Alessandra" sort="Traini, Alessandra" uniqKey="Traini A" first="Alessandra" last="Traini">Alessandra Traini</name><affiliation wicri:level="1"><nlm:aff id="I4">Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA</wicri:regionArea><wicri:noRegion>Cambridgeshire CB10 1SA</wicri:noRegion></affiliation></author><author><name sortKey="Recupero, Giuseppe Reforgiato" sort="Recupero, Giuseppe Reforgiato" uniqKey="Recupero G" first="Giuseppe Reforgiato" last="Recupero">Giuseppe Reforgiato Recupero</name><affiliation wicri:level="1"><nlm:aff id="I1">Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania</wicri:regionArea><wicri:noRegion>Catania</wicri:noRegion></affiliation></author><author><name sortKey="Frusciante, Luigi" sort="Frusciante, Luigi" uniqKey="Frusciante L" first="Luigi" last="Frusciante">Luigi Frusciante</name><affiliation wicri:level="1"><nlm:aff id="I3">Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Chiusano, Maria Luisa" sort="Chiusano, Maria Luisa" uniqKey="Chiusano M" first="Maria Luisa" last="Chiusano">Maria Luisa Chiusano</name><affiliation wicri:level="1"><nlm:aff id="I3">Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC Plant Biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Glutathione S-transferases (GSTs) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. Anthocyanin pigments require GSTs for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. Anthocyanin accumulation in <italic>Citrus sinensis</italic> (L.) Osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of Sicilian blood oranges. In this paper we describe: i) the characterization of the GST gene family in <italic>C. sinensis</italic> through a systematic EST analysis; ii) the validation of the EST assembly by exploiting the genome sequences of <italic>C. sinensis</italic> and <italic>C. clementina</italic> and their genome annotations; iii) GST gene expression profiling in six tissues/organs and in two different sweet orange cultivars, Cadenera (common) and Moro (pigmented).</p></sec><sec><title>Results</title><p>We identified 61 GST transcripts, described the full- or partial-length nature of the sequences and assigned to each sequence the GST class membership exploiting a comparative approach and the classification scheme proposed for plant species<italic>.</italic> A total of 23 full-length sequences were defined. Fifty-four of the 61 transcripts were successfully aligned to the <italic>C. sinensis</italic> and <italic>C. clementina</italic> genomes. Tissue specific expression profiling demonstrated that the expression of some GST transcripts was 'tissue-affected' and cultivar specific. A comparative analysis of <italic>C. sinensis</italic> GSTs with those from other plant species was also considered. Data from the current analysis are accessible at <ext-link ext-link-type="uri" xlink:href="http://biosrv.cab.unina.it/citrusGST/">http://biosrv.cab.unina.it/citrusGST/</ext-link>, with the aim to provide a reference resource for <italic>C. sinensis</italic> GSTs.</p></sec><sec><title>Conclusions</title><p>This study aimed at the characterization of the GST gene family in <italic>C. sinensis</italic>. Based on expression patterns from two different cultivars and on sequence-comparative analyses, we also highlighted that two sequences, a Phi class GST and a Mapeg class GST, could be involved in the conjugation of anthocyanin pigments and in their transport into the vacuole, specifically in fruit flesh of the pigmented cultivar.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Alfenito, Mr" uniqKey="Alfenito M">MR Alfenito</name></author><author><name sortKey="Souer, E" uniqKey="Souer E">E Souer</name></author><author><name sortKey="Goodman, Cd" uniqKey="Goodman C">CD Goodman</name></author><author><name sortKey="Buell, R" uniqKey="Buell R">R Buell</name></author><author><name sortKey="Mol, J" uniqKey="Mol J">J Mol</name></author><author><name sortKey="Koes, R" uniqKey="Koes R">R Koes</name></author><author><name sortKey="Walbot, V" uniqKey="Walbot V">V 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<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Plant Biol</journal-id><journal-id journal-id-type="iso-abbrev">BMC Plant Biol</journal-id><journal-title-group><journal-title>BMC Plant Biology</journal-title></journal-title-group><issn pub-type="epub">1471-2229</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24490620</article-id><article-id pub-id-type="pmc">3922800</article-id><article-id pub-id-type="publisher-id">1471-2229-14-39</article-id><article-id pub-id-type="doi">10.1186/1471-2229-14-39</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of <italic>Citrus sinensis</italic> (L.) Osbeck</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Licciardello</surname><given-names>Concetta</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>concetta.licciardello@entecra.it</email></contrib><contrib contrib-type="author" id="A2"><name><surname>D’Agostino</surname><given-names>Nunzio</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>nunzio.dagostino@entecra.it</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Traini</surname><given-names>Alessandra</given-names></name><xref ref-type="aff" rid="I4">4</xref><email>alessandra.traini@sanger.ac.uk</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Recupero</surname><given-names>Giuseppe Reforgiato</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>giuseppe.reforgiato@entecra.it</email></contrib><contrib contrib-type="author" id="A5"><name><surname>Frusciante</surname><given-names>Luigi</given-names></name><xref ref-type="aff" rid="I3">3</xref><email>fruscian@unina.it</email></contrib><contrib contrib-type="author" corresp="yes" id="A6"><name><surname>Chiusano</surname><given-names>Maria Luisa</given-names></name><xref ref-type="aff" rid="I3">3</xref><email>chiusano@unina.it</email></contrib></contrib-group><aff id="I1"><label>1</label>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Agrumicoltura e le Colture Mediterranee (CRA-ACM), Corso Savoia 190, 95024 Acireale, Catania, Italy</aff><aff id="I2"><label>2</label>Consiglio per la Ricerca e la sperimentazione in Agricoltura - Centro di ricerca per l'Orticoltura (CRA-ORT), via Cavalleggeri 25, 84098 Pontecagnano, Salerno, Italy</aff><aff id="I3"><label>3</label>Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, Via Università, 100, 80055 Portici, Naples, Italy</aff><aff id="I4"><label>4</label>Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK</aff><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>3</day><month>2</month><year>2014</year></pub-date><volume>14</volume><fpage>39</fpage><lpage>39</lpage><history><date date-type="received"><day>7</day><month>8</month><year>2013</year></date><date date-type="accepted"><day>20</day><month>12</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2014 Licciardello et al.; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Licciardello et al.; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an open access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.biomedcentral.com/1471-2229/14/39"></self-uri><abstract><sec><title>Background</title><p>Glutathione S-transferases (GSTs) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. Anthocyanin pigments require GSTs for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. Anthocyanin accumulation in <italic>Citrus sinensis</italic> (L.) Osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of Sicilian blood oranges. In this paper we describe: i) the characterization of the GST gene family in <italic>C. sinensis</italic> through a systematic EST analysis; ii) the validation of the EST assembly by exploiting the genome sequences of <italic>C. sinensis</italic> and <italic>C. clementina</italic> and their genome annotations; iii) GST gene expression profiling in six tissues/organs and in two different sweet orange cultivars, Cadenera (common) and Moro (pigmented).</p></sec><sec><title>Results</title><p>We identified 61 GST transcripts, described the full- or partial-length nature of the sequences and assigned to each sequence the GST class membership exploiting a comparative approach and the classification scheme proposed for plant species<italic>.</italic> A total of 23 full-length sequences were defined. Fifty-four of the 61 transcripts were successfully aligned to the <italic>C. sinensis</italic> and <italic>C. clementina</italic> genomes. Tissue specific expression profiling demonstrated that the expression of some GST transcripts was 'tissue-affected' and cultivar specific. A comparative analysis of <italic>C. sinensis</italic> GSTs with those from other plant species was also considered. Data from the current analysis are accessible at <ext-link ext-link-type="uri" xlink:href="http://biosrv.cab.unina.it/citrusGST/">http://biosrv.cab.unina.it/citrusGST/</ext-link>, with the aim to provide a reference resource for <italic>C. sinensis</italic> GSTs.</p></sec><sec><title>Conclusions</title><p>This study aimed at the characterization of the GST gene family in <italic>C. sinensis</italic>. Based on expression patterns from two different cultivars and on sequence-comparative analyses, we also highlighted that two sequences, a Phi class GST and a Mapeg class GST, could be involved in the conjugation of anthocyanin pigments and in their transport into the vacuole, specifically in fruit flesh of the pigmented cultivar.</p></sec></abstract><kwd-group><kwd>Sweet orange</kwd><kwd>GST</kwd><kwd>Expressed sequence tag</kwd><kwd>Gene family</kwd><kwd>Anthocyanin vacuolarization</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Italie</li><li>Royaume-Uni</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Licciardello, Concetta" sort="Licciardello, Concetta" uniqKey="Licciardello C" first="Concetta" last="Licciardello">Concetta Licciardello</name></noRegion><name sortKey="Chiusano, Maria Luisa" sort="Chiusano, Maria Luisa" uniqKey="Chiusano M" first="Maria Luisa" last="Chiusano">Maria Luisa Chiusano</name><name sortKey="D Gostino, Nunzio" sort="D Gostino, Nunzio" uniqKey="D Gostino N" first="Nunzio" last="D Gostino">Nunzio D Gostino</name><name sortKey="Frusciante, Luigi" sort="Frusciante, Luigi" uniqKey="Frusciante L" first="Luigi" last="Frusciante">Luigi Frusciante</name><name sortKey="Recupero, Giuseppe Reforgiato" sort="Recupero, Giuseppe Reforgiato" uniqKey="Recupero G" first="Giuseppe Reforgiato" last="Recupero">Giuseppe Reforgiato Recupero</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Traini, Alessandra" sort="Traini, Alessandra" uniqKey="Traini A" first="Alessandra" last="Traini">Alessandra Traini</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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