Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of Aspergillus niger
Identifieur interne : 000868 ( Pmc/Checkpoint ); précédent : 000867; suivant : 000869Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of Aspergillus niger
Auteurs : Joosu Kuivanen [Finlande] ; Hugo Dantas [Finlande] ; Dominik Mojzita [Finlande] ; Edgar Mallmann [Brésil] ; Alessandra Biz [Brésil] ; Nadia Krieger [Brésil] ; David Mitchell [Brésil] ; Peter Richard [Finlande]Source :
- AMB Express [ 2191-0855 ] ; 2014.
Abstract
Citrus processing waste is a leftover from the citrus processing industry and is available in large amounts. Typically, this waste is dried to produce animal feed, but sometimes it is just dumped. Its main component is the peel, which consists mostly of pectin, with D-galacturonic acid as the main monomer.
Url:
DOI: 10.1186/s13568-014-0033-z
PubMed: 24949267
PubMed Central: 4052776
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4052776Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of <italic>Aspergillus niger</italic></title><author><name sortKey="Kuivanen, Joosu" sort="Kuivanen, Joosu" uniqKey="Kuivanen J" first="Joosu" last="Kuivanen">Joosu Kuivanen</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author><author><name sortKey="Dantas, Hugo" sort="Dantas, Hugo" uniqKey="Dantas H" first="Hugo" last="Dantas">Hugo Dantas</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author><author><name sortKey="Mojzita, Dominik" sort="Mojzita, Dominik" uniqKey="Mojzita D" first="Dominik" last="Mojzita">Dominik Mojzita</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author><author><name sortKey="Mallmann, Edgar" sort="Mallmann, Edgar" uniqKey="Mallmann E" first="Edgar" last="Mallmann">Edgar Mallmann</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Biz, Alessandra" sort="Biz, Alessandra" uniqKey="Biz A" first="Alessandra" last="Biz">Alessandra Biz</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Krieger, Nadia" sort="Krieger, Nadia" uniqKey="Krieger N" first="Nadia" last="Krieger">Nadia Krieger</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Mitchell, David" sort="Mitchell, David" uniqKey="Mitchell D" first="David" last="Mitchell">David Mitchell</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Richard, Peter" sort="Richard, Peter" uniqKey="Richard P" first="Peter" last="Richard">Peter Richard</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24949267</idno><idno type="pmc">4052776</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4052776</idno><idno type="RBID">PMC:4052776</idno><idno type="doi">10.1186/s13568-014-0033-z</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000A85</idno><idno type="wicri:Area/Pmc/Curation">000A84</idno><idno type="wicri:Area/Pmc/Checkpoint">000868</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of <italic>Aspergillus niger</italic></title><author><name sortKey="Kuivanen, Joosu" sort="Kuivanen, Joosu" uniqKey="Kuivanen J" first="Joosu" last="Kuivanen">Joosu Kuivanen</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author><author><name sortKey="Dantas, Hugo" sort="Dantas, Hugo" uniqKey="Dantas H" first="Hugo" last="Dantas">Hugo Dantas</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author><author><name sortKey="Mojzita, Dominik" sort="Mojzita, Dominik" uniqKey="Mojzita D" first="Dominik" last="Mojzita">Dominik Mojzita</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author><author><name sortKey="Mallmann, Edgar" sort="Mallmann, Edgar" uniqKey="Mallmann E" first="Edgar" last="Mallmann">Edgar Mallmann</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Biz, Alessandra" sort="Biz, Alessandra" uniqKey="Biz A" first="Alessandra" last="Biz">Alessandra Biz</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Krieger, Nadia" sort="Krieger, Nadia" uniqKey="Krieger N" first="Nadia" last="Krieger">Nadia Krieger</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Mitchell, David" sort="Mitchell, David" uniqKey="Mitchell D" first="David" last="Mitchell">David Mitchell</name><affiliation wicri:level="1"><nlm:aff id="I2">Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</nlm:aff><country xml:lang="fr">Brésil</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990</wicri:regionArea><wicri:noRegion>81531-990</wicri:noRegion></affiliation></author><author><name sortKey="Richard, Peter" sort="Richard, Peter" uniqKey="Richard P" first="Peter" last="Richard">Peter Richard</name><affiliation wicri:level="1"><nlm:aff id="I1">VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>VTT Technical Research Centre of Finland, Espoo, 02044 VTT</wicri:regionArea><wicri:noRegion>02044 VTT</wicri:noRegion></affiliation></author></analytic><series><title level="j">AMB Express</title><idno type="eISSN">2191-0855</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Citrus processing waste is a leftover from the citrus processing industry and is available in large amounts. Typically, this waste is dried to produce animal feed, but sometimes it is just dumped. Its main component is the peel, which consists mostly of pectin, with D-galacturonic acid as the main monomer. <italic>Aspergillus niger</italic> is a filamentous fungus that efficiently produces pectinases for the hydrolysis of pectin and uses the resulting D-galacturonic acid and most of the other components of citrus peel for growth. We used engineered <italic>A. niger</italic> strains that were not able to catabolise D-galacturonic acid, but instead converted it to L-galactonic acid. These strains also produced pectinases for the hydrolysis of pectin and were used for the conversion of pectin in orange peel to L-galactonic acid in a consolidated process. The D-galacturonic acid in the orange peel was converted to L-galactonic acid with a yield close to 90%. Submerged and solid-state fermentation processes were compared.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Barratt, Rw" uniqKey="Barratt R">RW Barratt</name></author><author><name sortKey="Johnson, Gb" uniqKey="Johnson G">GB Johnson</name></author><author><name sortKey="Ogata, Wn" uniqKey="Ogata W">WN Ogata</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bremus, C" uniqKey="Bremus C">C Bremus</name></author><author><name sortKey="Herrmann, U" uniqKey="Herrmann U">U Herrmann</name></author><author><name sortKey="Bringer Meyer, S" uniqKey="Bringer Meyer S">S Bringer-Meyer</name></author><author><name sortKey="Sahm, H" uniqKey="Sahm H">H Sahm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Csiba, M" uniqKey="Csiba M">M Csiba</name></author><author><name sortKey="Cleophax, J" uniqKey="Cleophax J">J Cleophax</name></author><author><name sortKey="Petit, S" uniqKey="Petit S">S Petit</name></author><author><name sortKey="Gero, S" uniqKey="Gero S">S Gero</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Edwards, Mc" uniqKey="Edwards M">MC Edwards</name></author><author><name sortKey="Doran Peterson, J" uniqKey="Doran Peterson J">J Doran-Peterson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grohman, K" uniqKey="Grohman K">K Grohman</name></author><author><name sortKey="Cameron, R" uniqKey="Cameron R">R Cameron</name></author><author><name sortKey="Kim, Y" uniqKey="Kim Y">Y Kim</name></author><author><name sortKey="Widmer, W" uniqKey="Widmer W">W Widmer</name></author><author><name sortKey="Luzio, G" uniqKey="Luzio G">G Luzio</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hestrin, S" uniqKey="Hestrin S">S Hestrin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuivanen, J" uniqKey="Kuivanen J">J Kuivanen</name></author><author><name sortKey="Mojzita, D" uniqKey="Mojzita D">D Mojzita</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name></author><author><name sortKey="Hilditch, S" uniqKey="Hilditch S">S Hilditch</name></author><author><name sortKey="Penttil, M" uniqKey="Penttil M">M Penttilä</name></author><author><name sortKey="Richard, P" uniqKey="Richard P">P Richard</name></author><author><name sortKey="Wiebe, Mg" uniqKey="Wiebe M">MG Wiebe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Siles, Ja" uniqKey="Siles J">JA Siles</name></author><author><name sortKey="Thompson, Ip" uniqKey="Thompson I">IP Thompson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name></author><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author><author><name sortKey="Xing, J" uniqKey="Xing J">J Xing</name></author><author><name sortKey="Su, Z" uniqKey="Su Z">Z Su</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="L Pez, J" uniqKey="L Pez J">J López</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Thompson, I" uniqKey="Thompson I">I Thompson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martens Uzunova, Es" uniqKey="Martens Uzunova E">ES Martens-Uzunova</name></author><author><name sortKey="Schaap, Pj" uniqKey="Schaap P">PJ Schaap</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mojzita, D" uniqKey="Mojzita D">D Mojzita</name></author><author><name sortKey="Wiebe, M" uniqKey="Wiebe M">M Wiebe</name></author><author><name sortKey="Hilditch, S" uniqKey="Hilditch S">S Hilditch</name></author><author><name sortKey="Boer, H" uniqKey="Boer H">H Boer</name></author><author><name sortKey="Penttil, M" uniqKey="Penttil M">M Penttilä</name></author><author><name sortKey="Richard, P" uniqKey="Richard P">P Richard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Onofri, S" uniqKey="Onofri S">S Onofri</name></author><author><name sortKey="Poerio, E" uniqKey="Poerio E">E Poerio</name></author><author><name sortKey="Serangeli, P" uniqKey="Serangeli P">P Serangeli</name></author><author><name sortKey="Tosi, S" uniqKey="Tosi S">S Tosi</name></author><author><name sortKey="Garuccio, I" uniqKey="Garuccio I">I Garuccio</name></author><author><name sortKey="Arrigoni, O" uniqKey="Arrigoni O">O Arrigoni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pappenberger, G" uniqKey="Pappenberger G">G Pappenberger</name></author><author><name sortKey="Hohmann, H" uniqKey="Hohmann H">H Hohmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pourbafrani, M" uniqKey="Pourbafrani M">M Pourbafrani</name></author><author><name sortKey="Forgacs, G" uniqKey="Forgacs G">G Forgács</name></author><author><name sortKey="Horvath, Is" uniqKey="Horvath I">IS Horváth</name></author><author><name sortKey="Niklasson, C" uniqKey="Niklasson C">C Niklasson</name></author><author><name sortKey="Taherzadeh, Mj" uniqKey="Taherzadeh M">MJ Taherzadeh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reichstein, T" uniqKey="Reichstein T">T Reichstein</name></author><author><name sortKey="Grussner, A" uniqKey="Grussner A">A Grüssner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Richard, P" uniqKey="Richard P">P Richard</name></author><author><name sortKey="Hilditch, S" uniqKey="Hilditch S">S Hilditch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rivas, B" uniqKey="Rivas B">B Rivas</name></author><author><name sortKey="Torrado, A" uniqKey="Torrado A">A Torrado</name></author><author><name sortKey="Torre, P" uniqKey="Torre P">P Torre</name></author><author><name sortKey="Converti, A" uniqKey="Converti A">A Converti</name></author><author><name sortKey="Dominguez, Jm" uniqKey="Dominguez J">JM Domínguez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roland, Jf" uniqKey="Roland J">JF Roland</name></author><author><name sortKey="Cayle, T" uniqKey="Cayle T">T Cayle</name></author><author><name sortKey="Dinwoodie, Rc" uniqKey="Dinwoodie R">RC Dinwoodie</name></author><author><name sortKey="Mehnert, Dw" uniqKey="Mehnert D">DW Mehnert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romero Zaliz, Cl" uniqKey="Romero Zaliz C">CL Romero Zaliz</name></author><author><name sortKey="Varela, O" uniqKey="Varela O">O Varela</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romero Zaliz, Cl" uniqKey="Romero Zaliz C">CL Romero Zaliz</name></author><author><name sortKey="Varela, O" uniqKey="Varela O">O Varela</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sharma, N" uniqKey="Sharma N">N Sharma</name></author><author><name sortKey="Tripathi, A" uniqKey="Tripathi A">A Tripathi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Solis Pereira, S" uniqKey="Solis Pereira S">S Solis-Pereira</name></author><author><name sortKey="Favela Torres, E" uniqKey="Favela Torres E">E Favela-Torres</name></author><author><name sortKey="Viniegra Gonzalez, G" uniqKey="Viniegra Gonzalez G">G Viniegra-González</name></author><author><name sortKey="Gutierrez Rojas, M" uniqKey="Gutierrez Rojas M">M Gutiérrez-Rojas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Staunstrup, J" uniqKey="Staunstrup J">J Staunstrup</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Van Maris, Aja" uniqKey="Van Maris A">AJA Van Maris</name></author><author><name sortKey="Abbott, Da" uniqKey="Abbott D">DA Abbott</name></author><author><name sortKey="Bellissimi, E" uniqKey="Bellissimi E">E Bellissimi</name></author><author><name sortKey="Brink, J" uniqKey="Brink J">J Brink</name></author><author><name sortKey="Kuyper, M" uniqKey="Kuyper M">M Kuyper</name></author><author><name sortKey="Luttik, Mah" uniqKey="Luttik M">MAH Luttik</name></author><author><name sortKey="Wisselink, Hw" uniqKey="Wisselink H">HW Wisselink</name></author><author><name sortKey="Scheffers, Wa" uniqKey="Scheffers W">WA Scheffers</name></author><author><name sortKey="Dijken, Jp" uniqKey="Dijken J">JP Dijken</name></author><author><name sortKey="Pronk, Jt" uniqKey="Pronk J">JT Pronk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Viniegra Gonzalez, G" uniqKey="Viniegra Gonzalez G">G Viniegra-González</name></author><author><name sortKey="Favela Torres, E" uniqKey="Favela Torres E">E Favela-Torres</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Widmer, W" uniqKey="Widmer W">W Widmer</name></author><author><name sortKey="Zhou, W" uniqKey="Zhou W">W Zhou</name></author><author><name sortKey="Grohmann, K" uniqKey="Grohmann K">K Grohmann</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article" xml:lang="en"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">AMB Express</journal-id><journal-id journal-id-type="iso-abbrev">AMB Express</journal-id><journal-title-group><journal-title>AMB Express</journal-title></journal-title-group><issn pub-type="epub">2191-0855</issn><publisher><publisher-name>Springer</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24949267</article-id><article-id pub-id-type="pmc">4052776</article-id><article-id pub-id-type="publisher-id">s13568-014-0033-z</article-id><article-id pub-id-type="doi">10.1186/s13568-014-0033-z</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of <italic>Aspergillus niger</italic></article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes" id="A1"><name><surname>Kuivanen</surname><given-names>Joosu</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>joosu.kuivanen@vtt.fi</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Dantas</surname><given-names>Hugo</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>hugodantas03@gmail.com</email></contrib><contrib contrib-type="author" id="A3"><name><surname>Mojzita</surname><given-names>Dominik</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>dominik.mojzita@vtt.fi</email></contrib><contrib contrib-type="author" id="A4"><name><surname>Mallmann</surname><given-names>Edgar</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>edgar.mallmann@yahoo.com.br</email></contrib><contrib contrib-type="author" id="A5"><name><surname>Biz</surname><given-names>Alessandra</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>aless_biz@yahoo.com.br</email></contrib><contrib contrib-type="author" id="A6"><name><surname>Krieger</surname><given-names>Nadia</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>nkrieger@ufpr.br</email></contrib><contrib contrib-type="author" id="A7"><name><surname>Mitchell</surname><given-names>David</given-names></name><xref ref-type="aff" rid="I2">2</xref><email>davidmitchell@ufpr.br</email></contrib><contrib contrib-type="author" id="A8"><name><surname>Richard</surname><given-names>Peter</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>peter.richard@vtt.fi</email></contrib></contrib-group><aff id="I1"><label>1</label>VTT Technical Research Centre of Finland, Espoo, 02044 VTT, Finland</aff><aff id="I2"><label>2</label>Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR, 81531-990, Brazil</aff><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>18</day><month>3</month><year>2014</year></pub-date><volume>4</volume><fpage>33</fpage><lpage>33</lpage><history><date date-type="received"><day>29</day><month>1</month><year>2014</year></date><date date-type="accepted"><day>2</day><month>2</month><year>2014</year></date></history><permissions><copyright-statement>Copyright © 2014 Kuivanen et al.; licensee Springer</copyright-statement><copyright-year>2014</copyright-year><copyright-holder>Kuivanen et al.; licensee Springer</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproductionin any medium, provided the original work is properly credited.</license-p></license></permissions><self-uri xlink:href="http://www.amb-express.com/content/4/1/33"></self-uri><abstract><p>Citrus processing waste is a leftover from the citrus processing industry and is available in large amounts. Typically, this waste is dried to produce animal feed, but sometimes it is just dumped. Its main component is the peel, which consists mostly of pectin, with D-galacturonic acid as the main monomer. <italic>Aspergillus niger</italic> is a filamentous fungus that efficiently produces pectinases for the hydrolysis of pectin and uses the resulting D-galacturonic acid and most of the other components of citrus peel for growth. We used engineered <italic>A. niger</italic> strains that were not able to catabolise D-galacturonic acid, but instead converted it to L-galactonic acid. These strains also produced pectinases for the hydrolysis of pectin and were used for the conversion of pectin in orange peel to L-galactonic acid in a consolidated process. The D-galacturonic acid in the orange peel was converted to L-galactonic acid with a yield close to 90%. Submerged and solid-state fermentation processes were compared.</p></abstract><kwd-group><kwd>Citrus processing waste</kwd><kwd>Orange peel</kwd><kwd>Consolidated bioprocessing</kwd><kwd>L-galactonic acid</kwd><kwd>D-galacturonic acid</kwd><kwd>Aspergillus niger</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Brésil</li><li>Finlande</li></country></list><tree><country name="Finlande"><noRegion><name sortKey="Kuivanen, Joosu" sort="Kuivanen, Joosu" uniqKey="Kuivanen J" first="Joosu" last="Kuivanen">Joosu Kuivanen</name></noRegion><name sortKey="Dantas, Hugo" sort="Dantas, Hugo" uniqKey="Dantas H" first="Hugo" last="Dantas">Hugo Dantas</name><name sortKey="Mojzita, Dominik" sort="Mojzita, Dominik" uniqKey="Mojzita D" first="Dominik" last="Mojzita">Dominik Mojzita</name><name sortKey="Richard, Peter" sort="Richard, Peter" uniqKey="Richard P" first="Peter" last="Richard">Peter Richard</name></country><country name="Brésil"><noRegion><name sortKey="Mallmann, Edgar" sort="Mallmann, Edgar" uniqKey="Mallmann E" first="Edgar" last="Mallmann">Edgar Mallmann</name></noRegion><name sortKey="Biz, Alessandra" sort="Biz, Alessandra" uniqKey="Biz A" first="Alessandra" last="Biz">Alessandra Biz</name><name sortKey="Krieger, Nadia" sort="Krieger, Nadia" uniqKey="Krieger N" first="Nadia" last="Krieger">Nadia Krieger</name><name sortKey="Mitchell, David" sort="Mitchell, David" uniqKey="Mitchell D" first="David" last="Mitchell">David Mitchell</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000868 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000868 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:4052776
|texte= Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of Aspergillus niger
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:24949267" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |