Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures
Identifieur interne : 000848 ( Pmc/Checkpoint ); précédent : 000847; suivant : 000849Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures
Auteurs : Ewa Grzebelus [Pologne] ; Lukasz Skop [Pologne]Source :
- In Vitro Cellular & Developmental Biology [ 1054-5476 ] ; 2014.
Abstract
Protoplasts of three carrot cultivars were isolated from
Url:
DOI: 10.1007/s11627-014-9626-0
PubMed: 25298730
PubMed Central: 4182649
Affiliations:
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<author><name sortKey="Grzebelus, Ewa" sort="Grzebelus, Ewa" uniqKey="Grzebelus E" first="Ewa" last="Grzebelus">Ewa Grzebelus</name>
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<author><name sortKey="Skop, Lukasz" sort="Skop, Lukasz" uniqKey="Skop L" first="Lukasz" last="Skop">Lukasz Skop</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures</title>
<author><name sortKey="Grzebelus, Ewa" sort="Grzebelus, Ewa" uniqKey="Grzebelus E" first="Ewa" last="Grzebelus">Ewa Grzebelus</name>
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<author><name sortKey="Skop, Lukasz" sort="Skop, Lukasz" uniqKey="Skop L" first="Lukasz" last="Skop">Lukasz Skop</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Institute of Plant Biology and Biotechnology, Faculty of Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland</nlm:aff>
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<front><div type="abstract" xml:lang="en"><p>Protoplasts of three carrot cultivars were isolated from <italic>in vitro</italic>
-grown plantlets by overnight incubation in an enzyme mixture composed of 1% (<italic>w/v</italic>
) cellulase Onozuka R-10 and 0.1% (<italic>w/v</italic>
) pectolyase Y-23. After cell immobilization in modified thin alginate layers, three types of β-lactam antibiotics (cefotaxime, carbenicillin, or timentin) at five different concentrations (100, 200, 300, 400, or 500 mg L<sup>−1</sup>
) were added to the culture medium. In 20-d-old cultures, a different number of cell colonies had formed and varied on average from 27 to 56% in carbenicillin- and cefotaxime-containing media, respectively. Supplementation of the culture media with antibiotics at concentrations higher than 100 mg L<sup>−1</sup>
resulted in a decrease in plating efficiency in comparison with the controls. However, from all antibiotic treatments, except carbenicillin at concentrations of 400–500 mg L<sup>−1</sup>
, efficient plant regeneration occurred. For this reason, we believe that cefotaxime and timentin in the concentrations analyzed here may be used in complex <italic>in vitro</italic>
procedures or valuable carrot cultures as a prophylactic agent for prevention against occasional contaminations.</p>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">In Vitro Cell Dev Biol Plant</journal-id>
<journal-id journal-id-type="iso-abbrev">In Vitro Cell. Dev. Biol., Plant</journal-id>
<journal-title-group><journal-title>In Vitro Cellular & Developmental Biology</journal-title>
</journal-title-group>
<issn pub-type="ppub">1054-5476</issn>
<issn pub-type="epub">1475-2689</issn>
<publisher><publisher-name>Springer US</publisher-name>
<publisher-loc>Boston</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25298730</article-id>
<article-id pub-id-type="pmc">4182649</article-id>
<article-id pub-id-type="publisher-id">9626</article-id>
<article-id pub-id-type="doi">10.1007/s11627-014-9626-0</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Plant Development/Regeneration</subject>
</subj-group>
</article-categories>
<title-group><article-title>Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Grzebelus</surname>
<given-names>Ewa</given-names>
</name>
<address><email>e.grzebelus@ogr.ur.krakow.pl</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Skop</surname>
<given-names>Lukasz</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<aff id="Aff1">Institute of Plant Biology and Biotechnology, Faculty of Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland</aff>
</contrib-group>
<author-notes><fn fn-type="com"><p>Editor: John Forster</p>
</fn>
</author-notes>
<pub-date pub-type="epub"><day>4</day>
<month>7</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>4</day>
<month>7</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="ppub"><year>2014</year>
</pub-date>
<volume>50</volume>
<issue>5</issue>
<fpage>568</fpage>
<lpage>575</lpage>
<history><date date-type="received"><day>9</day>
<month>1</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>6</day>
<month>6</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>© The Society for In Vitro Biology 2014</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.</license-p>
</license>
</permissions>
<abstract id="Abs1"><p>Protoplasts of three carrot cultivars were isolated from <italic>in vitro</italic>
-grown plantlets by overnight incubation in an enzyme mixture composed of 1% (<italic>w/v</italic>
) cellulase Onozuka R-10 and 0.1% (<italic>w/v</italic>
) pectolyase Y-23. After cell immobilization in modified thin alginate layers, three types of β-lactam antibiotics (cefotaxime, carbenicillin, or timentin) at five different concentrations (100, 200, 300, 400, or 500 mg L<sup>−1</sup>
) were added to the culture medium. In 20-d-old cultures, a different number of cell colonies had formed and varied on average from 27 to 56% in carbenicillin- and cefotaxime-containing media, respectively. Supplementation of the culture media with antibiotics at concentrations higher than 100 mg L<sup>−1</sup>
resulted in a decrease in plating efficiency in comparison with the controls. However, from all antibiotic treatments, except carbenicillin at concentrations of 400–500 mg L<sup>−1</sup>
, efficient plant regeneration occurred. For this reason, we believe that cefotaxime and timentin in the concentrations analyzed here may be used in complex <italic>in vitro</italic>
procedures or valuable carrot cultures as a prophylactic agent for prevention against occasional contaminations.</p>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd><italic>Daucus carota</italic>
</kwd>
<kwd>Carbenicillin</kwd>
<kwd>Cefotaxime</kwd>
<kwd>Somatic embryogenesis</kwd>
<kwd>Timentin</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Society for In Vitro Biology 2014</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Pologne</li>
</country>
</list>
<tree><country name="Pologne"><noRegion><name sortKey="Grzebelus, Ewa" sort="Grzebelus, Ewa" uniqKey="Grzebelus E" first="Ewa" last="Grzebelus">Ewa Grzebelus</name>
</noRegion>
<name sortKey="Skop, Lukasz" sort="Skop, Lukasz" uniqKey="Skop L" first="Lukasz" last="Skop">Lukasz Skop</name>
</country>
</tree>
</affiliations>
</record>
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