Genome-Wide Identification and Expression Profile Analysis of Citrus Sucrose Synthase Genes: Investigation of Possible Roles in the Regulation of Sugar Accumulation
Identifieur interne : 000805 ( Pmc/Checkpoint ); précédent : 000804; suivant : 000806Genome-Wide Identification and Expression Profile Analysis of Citrus Sucrose Synthase Genes: Investigation of Possible Roles in the Regulation of Sugar Accumulation
Auteurs : Mohammad Zahidul Islam ; Xiao-Mei Hu ; Long-Fei Jin ; Yong-Zhong Liu ; Shu-Ang PengSource :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
Sucrose synthase (Sus) (EC 2.4.1.13) is a key enzyme for the sugar accumulation that is critical to form fruit quality. In this study, extensive data-mining and PCR amplification confirmed that there are at least six Sus genes (
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DOI: 10.1371/journal.pone.0113623
PubMed: 25420091
PubMed Central: 4242728
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genome-Wide Identification and Expression Profile Analysis of Citrus Sucrose Synthase Genes: Investigation of Possible Roles in the Regulation of Sugar Accumulation</title><author><name sortKey="Islam, Mohammad Zahidul" sort="Islam, Mohammad Zahidul" uniqKey="Islam M" first="Mohammad Zahidul" last="Islam">Mohammad Zahidul Islam</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Xiao Mei" sort="Hu, Xiao Mei" uniqKey="Hu X" first="Xiao-Mei" last="Hu">Xiao-Mei Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Jin, Long Fei" sort="Jin, Long Fei" uniqKey="Jin L" first="Long-Fei" last="Jin">Long-Fei Jin</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Liu, Yong Zhong" sort="Liu, Yong Zhong" uniqKey="Liu Y" first="Yong-Zhong" last="Liu">Yong-Zhong Liu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Peng, Shu Ang" sort="Peng, Shu Ang" uniqKey="Peng S" first="Shu-Ang" last="Peng">Shu-Ang Peng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25420091</idno><idno type="pmc">4242728</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4242728</idno><idno type="RBID">PMC:4242728</idno><idno type="doi">10.1371/journal.pone.0113623</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000221</idno><idno type="wicri:Area/Pmc/Curation">000221</idno><idno type="wicri:Area/Pmc/Checkpoint">000805</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genome-Wide Identification and Expression Profile Analysis of Citrus Sucrose Synthase Genes: Investigation of Possible Roles in the Regulation of Sugar Accumulation</title><author><name sortKey="Islam, Mohammad Zahidul" sort="Islam, Mohammad Zahidul" uniqKey="Islam M" first="Mohammad Zahidul" last="Islam">Mohammad Zahidul Islam</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hu, Xiao Mei" sort="Hu, Xiao Mei" uniqKey="Hu X" first="Xiao-Mei" last="Hu">Xiao-Mei Hu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Jin, Long Fei" sort="Jin, Long Fei" uniqKey="Jin L" first="Long-Fei" last="Jin">Long-Fei Jin</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Liu, Yong Zhong" sort="Liu, Yong Zhong" uniqKey="Liu Y" first="Yong-Zhong" last="Liu">Yong-Zhong Liu</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Peng, Shu Ang" sort="Peng, Shu Ang" uniqKey="Peng S" first="Shu-Ang" last="Peng">Shu-Ang Peng</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Sucrose synthase (Sus) (EC 2.4.1.13) is a key enzyme for the sugar accumulation that is critical to form fruit quality. In this study, extensive data-mining and PCR amplification confirmed that there are at least six Sus genes (<italic>CitSus</italic>1-6) in the citrus genome. Gene structure and phylogeny analysis showed an evolutionary consistency with other plant species. The six Sus genes contain 12–15 exons and 11–14 introns and were evenly distributed into the three plant Sus groups (<italic>CitSus</italic>1 and <italic>CitSus</italic>2 in the Sus I group, <italic>CitSus</italic>3 and <italic>CitSus</italic>6 in the Sus II group, and <italic>CitSus</italic>4 and <italic>CitSus</italic>5 in the Sus III group). Transcripts of these six CitSus genes were subsequently examined. For tissues and organs, <italic>CitSus</italic>1 and 2 were predominantly expressed in fruit juice sacs (JS) whereas <italic>CitSus</italic>3 and 4 were predominantly expressed in early leaves (immature leaves), and <italic>CitSus</italic>5 and 6 were predominantly expressed in fruit JS and in mature leaves. During fruit development, <italic>CitSus</italic>5 transcript increased significantly and <italic>CitSus</italic>6 transcript decreased significantly in fruit JS. In the fruit segment membrane (SM), the transcript levels of <italic>CitSus2</italic> and 5 were markedly higher and the abundant levels of <italic>CitSus</italic>3 and 6 gradually decreased. Moreover, transcript levels of <italic>CitSus</italic>1-4 examined were higher and the <italic>CitSus</italic>5 transcript level was lower in the fruit SM than in fruit JS, while <italic>CitSus</italic>6 had a similar transcript level in fruit JS and SM. In addition, transcripts of <italic>CitSus</italic>1-6 responded differently to dehydration in mature leaves or to mild drought stress in fruit JS and SM. Finally, the possible roles of Sus genes in the regulation of sugar accumulation are discussed; however, further study is required.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Koch, Ke" uniqKey="Koch K">KE Koch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Koch, K" uniqKey="Koch K">K Koch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, N" uniqKey="Jiang N">N Jiang</name></author><author><name sortKey="Jin, L F" uniqKey="Jin L">L-F Jin</name></author><author><name sortKey="Teixeira Da Silva, Ja" uniqKey="Teixeira Da Silva J">JA Teixeira da Silva</name></author><author><name sortKey="Islam, Mdz" uniqKey="Islam M">MDZ Islam</name></author><author><name sortKey="Gao, H W" uniqKey="Gao H">H-W Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, S M" uniqKey="Xu S">S-M Xu</name></author><author><name 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25420091</article-id><article-id pub-id-type="pmc">4242728</article-id><article-id pub-id-type="publisher-id">PONE-D-14-37456</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0113623</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology and life sciences</subject><subj-group><subject>Agriculture</subject><subj-group><subject>Crop Science</subject><subj-group><subject>Crops</subject><subj-group><subject>Fruits</subject><subj-group><subject>Citrus</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group><subject>Biotechnology</subject><subj-group><subject>Plant Biotechnology</subject><subj-group><subject>Plant Genomics</subject><subj-group><subject>Plant Genomes</subject></subj-group></subj-group></subj-group></subj-group><subj-group><subject>Ecology</subject><subj-group><subject>Plant Ecology</subject><subj-group><subject>Plant-Environment Interactions</subject><subj-group><subject>Plant Resistance to Abiotic Stress</subject><subj-group><subject>Drought Adaptation</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group><subject>Genetics</subject><subj-group><subject>Gene amplification</subject><subj-group><subject>RNA amplification</subject></subj-group></subj-group><subj-group><subject>Gene Expression</subject><subject>Gene Function</subject></subj-group></subj-group><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Physiology</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Genome-Wide Identification and Expression Profile Analysis of Citrus Sucrose Synthase Genes: Investigation of Possible Roles in the Regulation of Sugar Accumulation</article-title><alt-title alt-title-type="running-head">Citrus Sus Genes Identification and Transcript Analysis</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Islam</surname><given-names>Mohammad Zahidul</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="author-notes" rid="fn1"><sup>¤</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hu</surname><given-names>Xiao-Mei</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Jin</surname><given-names>Long-Fei</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Yong-Zhong</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib><contrib contrib-type="author"><name><surname>Peng</surname><given-names>Shu-Ang</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib></contrib-group><aff id="aff1"><addr-line>Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, Wuhan, People's Republic of China</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Han</surname><given-names>Yuepeng</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Chinese Academy of Sciences, China</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>liuyongzhong@mail.hzau.edu.cn</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: YZL SAP. Performed the experiments: MZI XMH LFJ. Analyzed the data: MZI YZL. Contributed reagents/materials/analysis tools: MZI XMH LFJ. Wrote the paper: MZI YZL.</p></fn><fn id="fn1" fn-type="current-aff"><label>¤</label><p>Current address: Bangladesh Water Development Board, Motijheel, Dhaka, Bangladesh</p></fn></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>24</day><month>11</month><year>2014</year></pub-date><volume>9</volume><issue>11</issue><elocation-id>e113623</elocation-id><history><date date-type="received"><day>3</day><month>9</month><year>2014</year></date><date date-type="accepted"><day>29</day><month>10</month><year>2014</year></date></history><permissions><copyright-year>2014</copyright-year><copyright-holder>Islam et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><p>Sucrose synthase (Sus) (EC 2.4.1.13) is a key enzyme for the sugar accumulation that is critical to form fruit quality. In this study, extensive data-mining and PCR amplification confirmed that there are at least six Sus genes (<italic>CitSus</italic>1-6) in the citrus genome. Gene structure and phylogeny analysis showed an evolutionary consistency with other plant species. The six Sus genes contain 12–15 exons and 11–14 introns and were evenly distributed into the three plant Sus groups (<italic>CitSus</italic>1 and <italic>CitSus</italic>2 in the Sus I group, <italic>CitSus</italic>3 and <italic>CitSus</italic>6 in the Sus II group, and <italic>CitSus</italic>4 and <italic>CitSus</italic>5 in the Sus III group). Transcripts of these six CitSus genes were subsequently examined. For tissues and organs, <italic>CitSus</italic>1 and 2 were predominantly expressed in fruit juice sacs (JS) whereas <italic>CitSus</italic>3 and 4 were predominantly expressed in early leaves (immature leaves), and <italic>CitSus</italic>5 and 6 were predominantly expressed in fruit JS and in mature leaves. During fruit development, <italic>CitSus</italic>5 transcript increased significantly and <italic>CitSus</italic>6 transcript decreased significantly in fruit JS. In the fruit segment membrane (SM), the transcript levels of <italic>CitSus2</italic> and 5 were markedly higher and the abundant levels of <italic>CitSus</italic>3 and 6 gradually decreased. Moreover, transcript levels of <italic>CitSus</italic>1-4 examined were higher and the <italic>CitSus</italic>5 transcript level was lower in the fruit SM than in fruit JS, while <italic>CitSus</italic>6 had a similar transcript level in fruit JS and SM. In addition, transcripts of <italic>CitSus</italic>1-6 responded differently to dehydration in mature leaves or to mild drought stress in fruit JS and SM. Finally, the possible roles of Sus genes in the regulation of sugar accumulation are discussed; however, further study is required.</p></abstract><funding-group><funding-statement>This work was supported by Fundamental Research Funds for the Central Universities in China (Grant No. 2013PY082) YZL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="16"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files.</p></notes></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Hu, Xiao Mei" sort="Hu, Xiao Mei" uniqKey="Hu X" first="Xiao-Mei" last="Hu">Xiao-Mei Hu</name><name sortKey="Islam, Mohammad Zahidul" sort="Islam, Mohammad Zahidul" uniqKey="Islam M" first="Mohammad Zahidul" last="Islam">Mohammad Zahidul Islam</name><name sortKey="Jin, Long Fei" sort="Jin, Long Fei" uniqKey="Jin L" first="Long-Fei" last="Jin">Long-Fei Jin</name><name sortKey="Liu, Yong Zhong" sort="Liu, Yong Zhong" uniqKey="Liu Y" first="Yong-Zhong" last="Liu">Yong-Zhong Liu</name><name sortKey="Peng, Shu Ang" sort="Peng, Shu Ang" uniqKey="Peng S" first="Shu-Ang" last="Peng">Shu-Ang Peng</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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