Next generation haplotyping to decipher nuclear genomic interspecific admixture in Citrus species: analysis of chromosome 2
Identifieur interne : 000754 ( Pmc/Checkpoint ); précédent : 000753; suivant : 000755Next generation haplotyping to decipher nuclear genomic interspecific admixture in Citrus species: analysis of chromosome 2
Auteurs : Franck Curk [France, Espagne] ; Gema Ancillo [Espagne] ; Andres Garcia-Lor [Espagne] ; François Luro [France] ; Xavier Perrier ; Jean-Pierre Jacquemoud-Collet ; Luis Navarro [Espagne] ; Patrick Ollitrault [Espagne]Source :
- BMC Genetics [ 1471-2156 ] ; 2014.
Abstract
The most economically important
454 amplicon libraries were established using the Fluidigm array system for 48 genotypes and 16 gene fragments from chromosome 2. Haplotypes were established from the reads of each accession and phylogenetic analyses were performed using the haplotypic data for each gene fragment. The length of 454 reads and the level of differentiation between the ancestral taxa of modern citrus allowed efficient haplotype phylogenetic assignations for 12 of the 16 gene fragments. The analysis of the mixed genomic structure of modern species and cultivars (i) revealed
454 sequencing was an efficient strategy to establish haplotypes with significant phylogenetic assignations in
The online version of this article (doi:10.1186/s12863-014-0152-1) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12863-014-0152-1
PubMed: 25544367
PubMed Central: 4302129
Affiliations:
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PMC:4302129Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Next generation haplotyping to decipher nuclear genomic interspecific admixture in <italic>Citrus</italic>
species: analysis of chromosome 2</title>
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<country xml:lang="fr">France</country>
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</affiliation>
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<author><name sortKey="Luro, Francois" sort="Luro, Francois" uniqKey="Luro F" first="François" last="Luro">François Luro</name>
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<author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name>
<affiliation wicri:level="2"><nlm:aff id="Aff2">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia Spain</nlm:aff>
<country>Espagne</country>
<placeName><region type="province">Province de Valence</region>
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</analytic>
<series><title level="j">BMC Genetics</title>
<idno type="eISSN">1471-2156</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The most economically important <italic>Citrus</italic>
species originated by natural interspecific hybridization between four ancestral taxa (<italic>Citrus reticulata</italic>
, <italic>Citrus maxima</italic>
, <italic>Citrus medica</italic>
, and <italic>Citrus micrantha</italic>
) and from limited subsequent interspecific recombination as a result of apomixis and vegetative propagation. Such reticulate evolution coupled with vegetative propagation results in mosaic genomes with large chromosome fragments from the basic taxa in frequent interspecific heterozygosity. Modern breeding of these species is hampered by their complex heterozygous genomic structures that determine species phenotype and are broken by sexual hybridisation. Nevertheless, a large amount of diversity is present in the citrus gene pool, and breeding to allow inclusion of desirable traits is of paramount importance. However, the efficient mobilization of citrus biodiversity in innovative breeding schemes requires previous understanding of <italic>Citrus</italic>
origins and genomic structures. Haplotyping of multiple gene fragments along the whole genome is a powerful approach to reveal the admixture genomic structure of current species and to resolve the evolutionary history of the gene pools. In this study, the efficiency of parallel sequencing with 454 methodology to decipher the hybrid structure of modern citrus species was assessed by analysis of 16 gene fragments on chromosome 2.</p>
</sec>
<sec><title>Results</title>
<p>454 amplicon libraries were established using the Fluidigm array system for 48 genotypes and 16 gene fragments from chromosome 2. Haplotypes were established from the reads of each accession and phylogenetic analyses were performed using the haplotypic data for each gene fragment. The length of 454 reads and the level of differentiation between the ancestral taxa of modern citrus allowed efficient haplotype phylogenetic assignations for 12 of the 16 gene fragments. The analysis of the mixed genomic structure of modern species and cultivars (i) revealed <italic>C. maxima</italic>
introgressions in modern mandarins, (ii) was consistent with previous hypotheses regarding the origin of secondary species, and (iii) provided a new picture of the evolution of chromosome 2.</p>
</sec>
<sec><title>Conclusions</title>
<p>454 sequencing was an efficient strategy to establish haplotypes with significant phylogenetic assignations in <italic>Citrus</italic>
, providing a new picture of the mixed structure on chromosome 2 in 48 citrus genotypes.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s12863-014-0152-1) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name>
</author>
<author><name sortKey="Trivedi, P" uniqKey="Trivedi P">P Trivedi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grosser, Jw" uniqKey="Grosser J">JW Grosser</name>
</author>
<author><name sortKey="Dutt, M" uniqKey="Dutt M">M Dutt</name>
</author>
<author><name sortKey="Omar, A" uniqKey="Omar A">A Omar</name>
</author>
<author><name sortKey="Orbovic, V" uniqKey="Orbovic V">V Orbovic</name>
</author>
<author><name sortKey="Barthe, G" uniqKey="Barthe G">G Barthe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Texeira, Dc" uniqKey="Texeira D">DC Texeira</name>
</author>
<author><name sortKey="Ayres, J" uniqKey="Ayres J">J Ayres</name>
</author>
<author><name sortKey="Kitajima, Ew" uniqKey="Kitajima E">EW Kitajima</name>
</author>
<author><name sortKey="Danet, L" uniqKey="Danet L">L Danet</name>
</author>
<author><name sortKey="Jagoueix Eveillard, S" uniqKey="Jagoueix Eveillard S">S Jagoueix-Eveillard</name>
</author>
<author><name sortKey="Saillard, C" uniqKey="Saillard C">C Saillard</name>
</author>
<author><name sortKey="Bove, Jm" uniqKey="Bove J">JM Bové</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Krueger, Rr" uniqKey="Krueger R">RR Krueger</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Scora, Rw" uniqKey="Scora R">RW Scora</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Swingle, Wt" uniqKey="Swingle W">WT Swingle</name>
</author>
<author><name sortKey="Reece, Pc" uniqKey="Reece P">PC Reece</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tanaka, T" uniqKey="Tanaka T">T Tanaka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mabberley, Dj" uniqKey="Mabberley D">DJ Mabberley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name>
</author>
<author><name sortKey="Fang, Dq" uniqKey="Fang D">DQ Fang</name>
</author>
<author><name sortKey="Scora, Rw" uniqKey="Scora R">RW Scora</name>
</author>
<author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nicolosi, E" uniqKey="Nicolosi E">E Nicolosi</name>
</author>
<author><name sortKey="Deng, Zn" uniqKey="Deng Z">ZN Deng</name>
</author>
<author><name sortKey="Gentile, A" uniqKey="Gentile A">A Gentile</name>
</author>
<author><name sortKey="La Malfa, S" uniqKey="La Malfa S">S La Malfa</name>
</author>
<author><name sortKey="Continella, G" uniqKey="Continella G">G Continella</name>
</author>
<author><name sortKey="Tribulato, E" uniqKey="Tribulato E">E Tribulato</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barkley, Na" uniqKey="Barkley N">NA Barkley</name>
</author>
<author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
<author><name sortKey="Krueger, Rr" uniqKey="Krueger R">RR Krueger</name>
</author>
<author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name>
</author>
<author><name sortKey="Curk, F" uniqKey="Curk F">F Curk</name>
</author>
<author><name sortKey="Snoussi Trifa, H" uniqKey="Snoussi Trifa H">H Snoussi-Trifa</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Ancillo, G" uniqKey="Ancillo G">G Ancillo</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name>
</author>
<author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name>
</author>
<author><name sortKey="Lotfy, S" uniqKey="Lotfy S">S Lotfy</name>
</author>
<author><name sortKey="Hippolyte, I" uniqKey="Hippolyte I">I Hippolyte</name>
</author>
<author><name sortKey="Ollitrault, F" uniqKey="Ollitrault F">F Ollitrault</name>
</author>
<author><name sortKey="Berard, A" uniqKey="Berard A">A Berard</name>
</author>
<author><name sortKey="Chauveau, A" uniqKey="Chauveau A">A Chauveau</name>
</author>
<author><name sortKey="Cuenca, J" uniqKey="Cuenca J">J Cuenca</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Kacar, Y" uniqKey="Kacar Y">Y Kacar</name>
</author>
<author><name sortKey="Mu, L" uniqKey="Mu L">L Mu</name>
</author>
<author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name>
</author>
<author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Aleza, P" uniqKey="Aleza P">P Aleza</name>
</author>
<author><name sortKey="Boland, A" uniqKey="Boland A">A Boland</name>
</author>
<author><name sortKey="Billot, C" uniqKey="Billot C">C Billot</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
<author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
<author><name sortKey="Brunel, D" uniqKey="Brunel D">D Brunel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name>
</author>
<author><name sortKey="Berard, A" uniqKey="Berard A">A Berard</name>
</author>
<author><name sortKey="Chauveau, A" uniqKey="Chauveau A">A Chauveau</name>
</author>
<author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Belzile, C" uniqKey="Belzile C">C Belzile</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Brunel, D" uniqKey="Brunel D">D Brunel</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Webber, Hj" uniqKey="Webber H">HJ Webber</name>
</author>
<author><name sortKey="Reuther, W" uniqKey="Reuther W">W Reuther</name>
</author>
<author><name sortKey="Lawton, Hw" uniqKey="Lawton H">HW Lawton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Barrett, Hc" uniqKey="Barrett H">HC Barrett</name>
</author>
<author><name sortKey="Rhodes, Am" uniqKey="Rhodes A">AM Rhodes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
<author><name sortKey="Jacquemond, C" uniqKey="Jacquemond C">C Jacquemond</name>
</author>
<author><name sortKey="Dubois, C" uniqKey="Dubois C">C Dubois</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Gatto, J" uniqKey="Gatto J">J Gatto</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Pailly, O" uniqKey="Pailly O">O Pailly</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fanciullino, Al" uniqKey="Fanciullino A">AL Fanciullino</name>
</author>
<author><name sortKey="Dhuique Mayer, C" uniqKey="Dhuique Mayer C">C Dhuique-Mayer</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Casanova, J" uniqKey="Casanova J">J Casanova</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stebbins, G" uniqKey="Stebbins G">G Stebbins</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grant, V" uniqKey="Grant V">V Grant</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Arnold, Ml" uniqKey="Arnold M">ML Arnold</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Doolittle, Wf" uniqKey="Doolittle W">WF Doolittle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Otto, Sp" uniqKey="Otto S">SP Otto</name>
</author>
<author><name sortKey="Whitton, J" uniqKey="Whitton J">J Whitton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Linder, Cr" uniqKey="Linder C">CR Linder</name>
</author>
<author><name sortKey="Rieseberg, Lh" uniqKey="Rieseberg L">LH Rieseberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pamilo, P" uniqKey="Pamilo P">P Pamilo</name>
</author>
<author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rieseberg, Lh" uniqKey="Rieseberg L">LH Rieseberg</name>
</author>
<author><name sortKey="Soltis, De" uniqKey="Soltis D">DE Soltis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Beiko, Rg" uniqKey="Beiko R">RG Beiko</name>
</author>
<author><name sortKey="Hamilton, N" uniqKey="Hamilton N">N Hamilton</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Rieseberg, Lh" uniqKey="Rieseberg L">LH Rieseberg</name>
</author>
<author><name sortKey="Raymond, O" uniqKey="Raymond O">O Raymond</name>
</author>
<author><name sortKey="Rosenthal, Dm" uniqKey="Rosenthal D">DM Rosenthal</name>
</author>
<author><name sortKey="Lai, Z" uniqKey="Lai Z">Z Lai</name>
</author>
<author><name sortKey="Livingstone, K" uniqKey="Livingstone K">K Livingstone</name>
</author>
<author><name sortKey="Nakazato, T" uniqKey="Nakazato T">T Nakazato</name>
</author>
<author><name sortKey="Durphy, Jl" uniqKey="Durphy J">JL Durphy</name>
</author>
<author><name sortKey="Schwarzbach, Ae" uniqKey="Schwarzbach A">AE Schwarzbach</name>
</author>
<author><name sortKey="Donovan, La" uniqKey="Donovan L">LA Donovan</name>
</author>
<author><name sortKey="Lexer, C" uniqKey="Lexer C">C Lexer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rousseau Gueutin, M" uniqKey="Rousseau Gueutin M">M Rousseau-Gueutin</name>
</author>
<author><name sortKey="Gaston, A" uniqKey="Gaston A">A Gaston</name>
</author>
<author><name sortKey="Ainouche, A" uniqKey="Ainouche A">A Aïnouche</name>
</author>
<author><name sortKey="Ainouche, Ml" uniqKey="Ainouche M">ML Aïnouche</name>
</author>
<author><name sortKey="Olbricht, K" uniqKey="Olbricht K">K Olbricht</name>
</author>
<author><name sortKey="Staudt, G" uniqKey="Staudt G">G Staudt</name>
</author>
<author><name sortKey="Richard, L" uniqKey="Richard L">L Richard</name>
</author>
<author><name sortKey="Denoyes Rothan, B" uniqKey="Denoyes Rothan B">B Denoyes-Rothan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fortune, P" uniqKey="Fortune P">P Fortune</name>
</author>
<author><name sortKey="Pourtau, N" uniqKey="Pourtau N">N Pourtau</name>
</author>
<author><name sortKey="Viron, N" uniqKey="Viron N">N Viron</name>
</author>
<author><name sortKey="Ainouche, M" uniqKey="Ainouche M">M Ainouche</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ramadugu, C" uniqKey="Ramadugu C">C Ramadugu</name>
</author>
<author><name sortKey="Pfeil, Be" uniqKey="Pfeil B">BE Pfeil</name>
</author>
<author><name sortKey="Keremane, Ml" uniqKey="Keremane M">ML Keremane</name>
</author>
<author><name sortKey="Lee, Rf" uniqKey="Lee R">RF Lee</name>
</author>
<author><name sortKey="Maureira Butler, Ij" uniqKey="Maureira Butler I">IJ Maureira-Butler</name>
</author>
<author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maddison, Wp" uniqKey="Maddison W">WP Maddison</name>
</author>
<author><name sortKey="Knowles, Ll" uniqKey="Knowles L">LL Knowles</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jumpponen, A" uniqKey="Jumpponen A">A Jumpponen</name>
</author>
<author><name sortKey="Jones, Kl" uniqKey="Jones K">KL Jones</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="S Nsteb, Jh" uniqKey="S Nsteb J">JH Sønstebø</name>
</author>
<author><name sortKey="Gielly, L" uniqKey="Gielly L">L Gielly</name>
</author>
<author><name sortKey="Brysting, Ak" uniqKey="Brysting A">AK Brysting</name>
</author>
<author><name sortKey="Elven, R" uniqKey="Elven R">R Elven</name>
</author>
<author><name sortKey="Edwards, M" uniqKey="Edwards M">M Edwards</name>
</author>
<author><name sortKey="Haile, J" uniqKey="Haile J">J Haile</name>
</author>
<author><name sortKey="Willersleve, E" uniqKey="Willersleve E">E Willersleve</name>
</author>
<author><name sortKey="Coissac, E" uniqKey="Coissac E">E Coissac</name>
</author>
<author><name sortKey="Rioux, D" uniqKey="Rioux D">D Rioux</name>
</author>
<author><name sortKey="Sannier, J" uniqKey="Sannier J">J Sannier</name>
</author>
<author><name sortKey="Taberlet, P" uniqKey="Taberlet P">P Taberlet</name>
</author>
<author><name sortKey="Brochmann, C" uniqKey="Brochmann C">C Brochmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Taudien, S" uniqKey="Taudien S">S Taudien</name>
</author>
<author><name sortKey="Groth, M" uniqKey="Groth M">M Groth</name>
</author>
<author><name sortKey="Huse, K" uniqKey="Huse K">K Huse</name>
</author>
<author><name sortKey="Petzold, A" uniqKey="Petzold A">A Petzold</name>
</author>
<author><name sortKey="Szafranski, K" uniqKey="Szafranski K">K Szafranski</name>
</author>
<author><name sortKey="Hampe, J" uniqKey="Hampe J">J Hampe</name>
</author>
<author><name sortKey="Rosenstiel, P" uniqKey="Rosenstiel P">P Rosenstiel</name>
</author>
<author><name sortKey="Schreiber, S" uniqKey="Schreiber S">S Schreiber</name>
</author>
<author><name sortKey="Platzer, M" uniqKey="Platzer M">M Platzer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xu, Q" uniqKey="Xu Q">Q Xu</name>
</author>
<author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name>
</author>
<author><name sortKey="Ruan, X" uniqKey="Ruan X">X Ruan</name>
</author>
<author><name sortKey="Chen, D" uniqKey="Chen D">D Chen</name>
</author>
<author><name sortKey="Zhu, A" uniqKey="Zhu A">A Zhu</name>
</author>
<author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name>
</author>
<author><name sortKey="Bertrand, D" uniqKey="Bertrand D">D Bertrand</name>
</author>
<author><name sortKey="Jiao, W" uniqKey="Jiao W">W Jiao</name>
</author>
<author><name sortKey="Hao, B" uniqKey="Hao B">B Hao</name>
</author>
<author><name sortKey="Lyon, Pm" uniqKey="Lyon P">PM Lyon</name>
</author>
<author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name>
</author>
<author><name sortKey="Gao, S" uniqKey="Gao S">S Gao</name>
</author>
<author><name sortKey="Xing, F" uniqKey="Xing F">F Xing</name>
</author>
<author><name sortKey="Lan, H" uniqKey="Lan H">H Lan</name>
</author>
<author><name sortKey="Chang, J" uniqKey="Chang J">J Chang</name>
</author>
<author><name sortKey="Ge, X" uniqKey="Ge X">X Ge</name>
</author>
<author><name sortKey="Lei, Y" uniqKey="Lei Y">Y Lei</name>
</author>
<author><name sortKey="Hu, Q" uniqKey="Hu Q">Q Hu</name>
</author>
<author><name sortKey="Miao, Y" uniqKey="Miao Y">Y Miao</name>
</author>
<author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name>
</author>
<author><name sortKey="Xiao, S" uniqKey="Xiao S">S Xiao</name>
</author>
<author><name sortKey="Biswas, Km" uniqKey="Biswas K">KM Biswas</name>
</author>
<author><name sortKey="Zeng, W" uniqKey="Zeng W">W Zeng</name>
</author>
<author><name sortKey="Guo, F" uniqKey="Guo F">F Guo</name>
</author>
<author><name sortKey="Cao, H" uniqKey="Cao H">H Cao</name>
</author>
<author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name>
</author>
<author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name>
</author>
<author><name sortKey="Cheng, Y" uniqKey="Cheng Y">Y Cheng</name>
</author>
<author><name sortKey="Xu, J" uniqKey="Xu J">J Xu</name>
</author>
<author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wu, Ga" uniqKey="Wu G">GA Wu</name>
</author>
<author><name sortKey="Prochnik, S" uniqKey="Prochnik S">S Prochnik</name>
</author>
<author><name sortKey="Jenkins, J" uniqKey="Jenkins J">J Jenkins</name>
</author>
<author><name sortKey="Salse, J" uniqKey="Salse J">J Salse</name>
</author>
<author><name sortKey="Hellsten, U" uniqKey="Hellsten U">U Hellsten</name>
</author>
<author><name sortKey="Murat, F" uniqKey="Murat F">F Murat</name>
</author>
<author><name sortKey="Perrier, X" uniqKey="Perrier X">X Perrier</name>
</author>
<author><name sortKey="Ruiz, M" uniqKey="Ruiz M">M Ruiz</name>
</author>
<author><name sortKey="Scalabrin, S" uniqKey="Scalabrin S">S Scalabrin</name>
</author>
<author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Takita, Ma" uniqKey="Takita M">MA Takita</name>
</author>
<author><name sortKey="Labadie, K" uniqKey="Labadie K">K Labadie</name>
</author>
<author><name sortKey="Poulain, J" uniqKey="Poulain J">J Poulain</name>
</author>
<author><name sortKey="Couloux, A" uniqKey="Couloux A">A Couloux</name>
</author>
<author><name sortKey="Jabbari, K" uniqKey="Jabbari K">K Jabbari</name>
</author>
<author><name sortKey="Cattonaro, F" uniqKey="Cattonaro F">F Cattonaro</name>
</author>
<author><name sortKey="Del Fabbro, C" uniqKey="Del Fabbro C">C Del Fabbro</name>
</author>
<author><name sortKey="Pinosio, S" uniqKey="Pinosio S">S Pinosio</name>
</author>
<author><name sortKey="Zuccolo, A" uniqKey="Zuccolo A">A Zuccolo</name>
</author>
<author><name sortKey="Chapman, J" uniqKey="Chapman J">J Chapman</name>
</author>
<author><name sortKey="Grimwood, J" uniqKey="Grimwood J">J Grimwood</name>
</author>
<author><name sortKey="Tadeo, Fr" uniqKey="Tadeo F">FR Tadeo</name>
</author>
<author><name sortKey="Estornell, Lh" uniqKey="Estornell L">LH Estornell</name>
</author>
<author><name sortKey="Munoz Sanz, Jv" uniqKey="Munoz Sanz J">JV Munoz-Sanz</name>
</author>
<author><name sortKey="Ibanez, V" uniqKey="Ibanez V">V Ibanez</name>
</author>
<author><name sortKey="Herrero Ortega, A" uniqKey="Herrero Ortega A">A Herrero-Ortega</name>
</author>
<author><name sortKey="Aleza, P" uniqKey="Aleza P">P Aleza</name>
</author>
<author><name sortKey="Perez Perez, J" uniqKey="Perez Perez J">J Perez-Perez</name>
</author>
<author><name sortKey="Ramon, D" uniqKey="Ramon D">D Ramon</name>
</author>
<author><name sortKey="Brunel, D" uniqKey="Brunel D">D Brunel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Okou, Dt" uniqKey="Okou D">DT Okou</name>
</author>
<author><name sortKey="Steinberg, Km" uniqKey="Steinberg K">KM Steinberg</name>
</author>
<author><name sortKey="Middle, C" uniqKey="Middle C">C Middle</name>
</author>
<author><name sortKey="Cutler, Dj" uniqKey="Cutler D">DJ Cutler</name>
</author>
<author><name sortKey="Albert, Tj" uniqKey="Albert T">TJ Albert</name>
</author>
<author><name sortKey="Zwick, Me" uniqKey="Zwick M">ME Zwick</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bybee, Sm" uniqKey="Bybee S">SM Bybee</name>
</author>
<author><name sortKey="Bracken Grissom, H" uniqKey="Bracken Grissom H">H Bracken-Grissom</name>
</author>
<author><name sortKey="Haynes, Bd" uniqKey="Haynes B">BD Haynes</name>
</author>
<author><name sortKey="Hermansen, Ra" uniqKey="Hermansen R">RA Hermansen</name>
</author>
<author><name sortKey="Byers, Rl" uniqKey="Byers R">RL Byers</name>
</author>
<author><name sortKey="Clement, Mj" uniqKey="Clement M">MJ Clement</name>
</author>
<author><name sortKey="Udall, Ja" uniqKey="Udall J">JA Udall</name>
</author>
<author><name sortKey="Wilcox, Er" uniqKey="Wilcox E">ER Wilcox</name>
</author>
<author><name sortKey="Crandall, Ka" uniqKey="Crandall K">KA Crandall</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Wright, S" uniqKey="Wright S">S Wright</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Dereeper, A" uniqKey="Dereeper A">A Dereeper</name>
</author>
<author><name sortKey="Nicolas, S" uniqKey="Nicolas S">S Nicolas</name>
</author>
<author><name sortKey="Lecunff, L" uniqKey="Lecunff L">L Lecunff</name>
</author>
<author><name sortKey="Bacilieri, R" uniqKey="Bacilieri R">R Bacilieri</name>
</author>
<author><name sortKey="Doligez, A" uniqKey="Doligez A">A Doligez</name>
</author>
<author><name sortKey="Peros, Jp" uniqKey="Peros J">JP Peros</name>
</author>
<author><name sortKey="Ruiz, M" uniqKey="Ruiz M">M Ruiz</name>
</author>
<author><name sortKey="This, P" uniqKey="This P">P This</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name>
</author>
<author><name sortKey="Stecher, G" uniqKey="Stecher G">G Stecher</name>
</author>
<author><name sortKey="Peterson, D" uniqKey="Peterson D">D Peterson</name>
</author>
<author><name sortKey="Filipski, A" uniqKey="Filipski A">A Filipski</name>
</author>
<author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Berloo, R" uniqKey="Van Berloo R">R Van Berloo</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Pritchard, Jk" uniqKey="Pritchard J">JK Pritchard</name>
</author>
<author><name sortKey="Stephens, M" uniqKey="Stephens M">M Stephens</name>
</author>
<author><name sortKey="Donnelly, P" uniqKey="Donnelly P">P Donnelly</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Falush, D" uniqKey="Falush D">D Falush</name>
</author>
<author><name sortKey="Stephens, M" uniqKey="Stephens M">M Stephens</name>
</author>
<author><name sortKey="Pritchard, Jk" uniqKey="Pritchard J">JK Pritchard</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Evanno, G" uniqKey="Evanno G">G Evanno</name>
</author>
<author><name sortKey="Regnaut, S" uniqKey="Regnaut S">S Regnaut</name>
</author>
<author><name sortKey="Goudet, J" uniqKey="Goudet J">J Goudet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Herrero, R" uniqKey="Herrero R">R Herrero</name>
</author>
<author><name sortKey="Asins, Mj" uniqKey="Asins M">MJ Asins</name>
</author>
<author><name sortKey="Pina, Ja" uniqKey="Pina J">JA Pina</name>
</author>
<author><name sortKey="Carbonell, Ea" uniqKey="Carbonell E">EA Carbonell</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name>
</author>
<author><name sortKey="Mouhaya, W" uniqKey="Mouhaya W">W Mouhaya</name>
</author>
<author><name sortKey="Bassene, Jb" uniqKey="Bassene J">JB Bassene</name>
</author>
<author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name>
</author>
<author><name sortKey="Kamiri, M" uniqKey="Kamiri M">M Kamiri</name>
</author>
<author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name>
</author>
<author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Uzun, A" uniqKey="Uzun A">A Uzun</name>
</author>
<author><name sortKey="Yesiloglu, T" uniqKey="Yesiloglu T">T Yesiloglu</name>
</author>
<author><name sortKey="Polat, I" uniqKey="Polat I">I Polat</name>
</author>
<author><name sortKey="Aka Kacar, Y" uniqKey="Aka Kacar Y">Y Aka-Kacar</name>
</author>
<author><name sortKey="Gulsen, O" uniqKey="Gulsen O">O Gulsen</name>
</author>
<author><name sortKey="Yildirim, B" uniqKey="Yildirim B">B Yildirim</name>
</author>
<author><name sortKey="Tuzcu, O" uniqKey="Tuzcu O">O Tuzcu</name>
</author>
<author><name sortKey="Tepe, S" uniqKey="Tepe S">S Tepe</name>
</author>
<author><name sortKey="Canan, I" uniqKey="Canan I">I Canan</name>
</author>
<author><name sortKey="Anil, S" uniqKey="Anil S">S Anil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Uzun, A" uniqKey="Uzun A">A Uzun</name>
</author>
<author><name sortKey="Yesiloglu, T" uniqKey="Yesiloglu T">T Yesiloglu</name>
</author>
<author><name sortKey="Aka Kacar, Y" uniqKey="Aka Kacar Y">Y Aka-Kacar</name>
</author>
<author><name sortKey="Tuzcu, O" uniqKey="Tuzcu O">O Tuzcu</name>
</author>
<author><name sortKey="Gulsen, O" uniqKey="Gulsen O">O Gulsen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bayer, Rj" uniqKey="Bayer R">RJ Bayer</name>
</author>
<author><name sortKey="Mabberley, Dj" uniqKey="Mabberley D">DJ Mabberley</name>
</author>
<author><name sortKey="Morton, C" uniqKey="Morton C">C Morton</name>
</author>
<author><name sortKey="Miller, Ch" uniqKey="Miller C">CH Miller</name>
</author>
<author><name sortKey="Sharma, Ik" uniqKey="Sharma I">IK Sharma</name>
</author>
<author><name sortKey="Pfeil, Be" uniqKey="Pfeil B">BE Pfeil</name>
</author>
<author><name sortKey="Rich, S" uniqKey="Rich S">S Rich</name>
</author>
<author><name sortKey="Hitchcock, R" uniqKey="Hitchcock R">R Hitchcock</name>
</author>
<author><name sortKey="Sykes, S" uniqKey="Sykes S">S Sykes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
<author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name>
</author>
<author><name sortKey="Mu, L" uniqKey="Mu L">L Mu</name>
</author>
<author><name sortKey="Kwok, K" uniqKey="Kwok K">K Kwok</name>
</author>
<author><name sortKey="Vu, C" uniqKey="Vu C">C Vu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ollitrault, F" uniqKey="Ollitrault F">F Ollitrault</name>
</author>
<author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name>
</author>
<author><name sortKey="Pina, Ja" uniqKey="Pina J">JA Pina</name>
</author>
<author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name>
</author>
<author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name>
</author>
<author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Webber, Hj" uniqKey="Webber H">HJ Webber</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Scora, Rw" uniqKey="Scora R">RW Scora</name>
</author>
<author><name sortKey="Kumamoto, J" uniqKey="Kumamoto J">J Kumamoto</name>
</author>
<author><name sortKey="Soost, Rk" uniqKey="Soost R">RK Soost</name>
</author>
<author><name sortKey="Nauer, Em" uniqKey="Nauer E">EM Nauer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gallesio, G" uniqKey="Gallesio G">G Gallesio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Lg" uniqKey="Chen L">LG Chen</name>
</author>
<author><name sortKey="Omura, M" uniqKey="Omura M">M Omura</name>
</author>
<author><name sortKey="Hidaka, T" uniqKey="Hidaka T">T Hidaka</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name>
</author>
<author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name>
</author>
<author><name sortKey="Scora, Rw" uniqKey="Scora R">RW Scora</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, X" uniqKey="Li X">X Li</name>
</author>
<author><name sortKey="Xie, R" uniqKey="Xie R">R Xie</name>
</author>
<author><name sortKey="Lu, Z" uniqKey="Lu Z">Z Lu</name>
</author>
<author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z Zhou</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Genet</journal-id>
<journal-id journal-id-type="iso-abbrev">BMC Genet</journal-id>
<journal-title-group><journal-title>BMC Genetics</journal-title>
</journal-title-group>
<issn pub-type="epub">1471-2156</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25544367</article-id>
<article-id pub-id-type="pmc">4302129</article-id>
<article-id pub-id-type="publisher-id">152</article-id>
<article-id pub-id-type="doi">10.1186/s12863-014-0152-1</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Next generation haplotyping to decipher nuclear genomic interspecific admixture in <italic>Citrus</italic>
species: analysis of chromosome 2</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Curk</surname>
<given-names>Franck</given-names>
</name>
<address><email>curk@corse.inra.fr</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Ancillo</surname>
<given-names>Gema</given-names>
</name>
<address><email>ancillo@ivia.es</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Garcia-Lor</surname>
<given-names>Andres</given-names>
</name>
<address><email>angarcia@ivia.es</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Luro</surname>
<given-names>François</given-names>
</name>
<address><email>luro@corse.inra.fr</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Perrier</surname>
<given-names>Xavier</given-names>
</name>
<address><email>xavier.perrier@cirad.fr</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jacquemoud-Collet</surname>
<given-names>Jean-Pierre</given-names>
</name>
<address><email>jean-pierre.jacquemoud-collet@cirad.fr</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Navarro</surname>
<given-names>Luis</given-names>
</name>
<address><email>lnavarro@ivia.es</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Ollitrault</surname>
<given-names>Patrick</given-names>
</name>
<address><email>ollitrault@cirad.fr</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<aff id="Aff1"><label></label>
UMR AGAP, Institut National de la Recherche Agronomique (Inra), Centre Inra de Corse, F-20230 San Giuliano, France</aff>
<aff id="Aff2"><label></label>
Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Valencia Spain</aff>
<aff id="Aff3"><label></label>
UMR AGAP, Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), TA A-108/02, 34398 Montpellier, Cedex 5 France</aff>
</contrib-group>
<pub-date pub-type="epub"><day>29</day>
<month>12</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>29</day>
<month>12</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="collection"><year>2014</year>
</pub-date>
<volume>15</volume>
<elocation-id>152</elocation-id>
<history><date date-type="received"><day>21</day>
<month>8</month>
<year>2014</year>
</date>
<date date-type="accepted"><day>11</day>
<month>12</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>© Curk et al.; licensee BioMed Central. 2014</copyright-statement>
<license license-type="open-access"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0">http://creativecommons.org/licenses/by/4.0</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>
) applies to the data made available in this article, unless otherwise stated.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p>The most economically important <italic>Citrus</italic>
species originated by natural interspecific hybridization between four ancestral taxa (<italic>Citrus reticulata</italic>
, <italic>Citrus maxima</italic>
, <italic>Citrus medica</italic>
, and <italic>Citrus micrantha</italic>
) and from limited subsequent interspecific recombination as a result of apomixis and vegetative propagation. Such reticulate evolution coupled with vegetative propagation results in mosaic genomes with large chromosome fragments from the basic taxa in frequent interspecific heterozygosity. Modern breeding of these species is hampered by their complex heterozygous genomic structures that determine species phenotype and are broken by sexual hybridisation. Nevertheless, a large amount of diversity is present in the citrus gene pool, and breeding to allow inclusion of desirable traits is of paramount importance. However, the efficient mobilization of citrus biodiversity in innovative breeding schemes requires previous understanding of <italic>Citrus</italic>
origins and genomic structures. Haplotyping of multiple gene fragments along the whole genome is a powerful approach to reveal the admixture genomic structure of current species and to resolve the evolutionary history of the gene pools. In this study, the efficiency of parallel sequencing with 454 methodology to decipher the hybrid structure of modern citrus species was assessed by analysis of 16 gene fragments on chromosome 2.</p>
</sec>
<sec><title>Results</title>
<p>454 amplicon libraries were established using the Fluidigm array system for 48 genotypes and 16 gene fragments from chromosome 2. Haplotypes were established from the reads of each accession and phylogenetic analyses were performed using the haplotypic data for each gene fragment. The length of 454 reads and the level of differentiation between the ancestral taxa of modern citrus allowed efficient haplotype phylogenetic assignations for 12 of the 16 gene fragments. The analysis of the mixed genomic structure of modern species and cultivars (i) revealed <italic>C. maxima</italic>
introgressions in modern mandarins, (ii) was consistent with previous hypotheses regarding the origin of secondary species, and (iii) provided a new picture of the evolution of chromosome 2.</p>
</sec>
<sec><title>Conclusions</title>
<p>454 sequencing was an efficient strategy to establish haplotypes with significant phylogenetic assignations in <italic>Citrus</italic>
, providing a new picture of the mixed structure on chromosome 2 in 48 citrus genotypes.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s12863-014-0152-1) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Phylogeny</kwd>
<kwd>Haplotype</kwd>
<kwd>Evolution</kwd>
<kwd>SNP</kwd>
<kwd>NGS</kwd>
<kwd>Genome admixture</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2014</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Espagne</li>
<li>France</li>
</country>
<region><li>Province de Valence</li>
</region>
</list>
<tree><noCountry><name sortKey="Jacquemoud Collet, Jean Pierre" sort="Jacquemoud Collet, Jean Pierre" uniqKey="Jacquemoud Collet J" first="Jean-Pierre" last="Jacquemoud-Collet">Jean-Pierre Jacquemoud-Collet</name>
<name sortKey="Perrier, Xavier" sort="Perrier, Xavier" uniqKey="Perrier X" first="Xavier" last="Perrier">Xavier Perrier</name>
</noCountry>
<country name="France"><noRegion><name sortKey="Curk, Franck" sort="Curk, Franck" uniqKey="Curk F" first="Franck" last="Curk">Franck Curk</name>
</noRegion>
<name sortKey="Luro, Francois" sort="Luro, Francois" uniqKey="Luro F" first="François" last="Luro">François Luro</name>
</country>
<country name="Espagne"><region name="Province de Valence"><name sortKey="Curk, Franck" sort="Curk, Franck" uniqKey="Curk F" first="Franck" last="Curk">Franck Curk</name>
</region>
<name sortKey="Ancillo, Gema" sort="Ancillo, Gema" uniqKey="Ancillo G" first="Gema" last="Ancillo">Gema Ancillo</name>
<name sortKey="Garcia Lor, Andres" sort="Garcia Lor, Andres" uniqKey="Garcia Lor A" first="Andres" last="Garcia-Lor">Andres Garcia-Lor</name>
<name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name>
<name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name>
</country>
</tree>
</affiliations>
</record>
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