Structural and Biochemical Analysis of the Hordeum vulgare L. HvGR-RBP1 Protein, a Glycine-Rich RNA-Binding Protein Involved in the Regulation of Barley Plant Development and Stress Response
Identifieur interne : 000700 ( Pmc/Checkpoint ); précédent : 000699; suivant : 000701Structural and Biochemical Analysis of the Hordeum vulgare L. HvGR-RBP1 Protein, a Glycine-Rich RNA-Binding Protein Involved in the Regulation of Barley Plant Development and Stress Response
Auteurs : Brian X0a P. Tripet ; Katelyn E. Mason ; Brian J. Eilers ; Jennifer Burns ; Paul Powell ; Andreas M. Fischer [États-Unis] ; Valérie CopiéSource :
- Biochemistry [ 0006-2960 ] ; 2014.
Abstract
The timing of whole-plant senescence
influences important agricultural
traits such as yield and grain protein content. Post-transcriptional
regulation by plant RNA-binding proteins is essential for proper control
of gene expression, development, and stress responses. Here, we report
the three-dimensional solution NMR structure and nucleic acid-binding
properties of the barley glycine-rich RNA-binding protein
Url:
DOI: 10.1021/bi5007223
PubMed: 25495582
PubMed Central: 4278681
Affiliations:
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PMC:4278681Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural and Biochemical Analysis of the <italic>Hordeum vulgare</italic>
L. <italic>Hv</italic>
GR-RBP1 Protein,
a Glycine-Rich RNA-Binding Protein Involved in the Regulation of Barley
Plant Development and Stress Response</title>
<author><name sortKey="Tripet, Brian X0a P" sort="Tripet, Brian X0a P" uniqKey="Tripet B" first="Brian X0a P." last="Tripet">Brian X0a P. Tripet</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mason, Katelyn E" sort="Mason, Katelyn E" uniqKey="Mason K" first="Katelyn E." last="Mason">Katelyn E. Mason</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Eilers, Brian J" sort="Eilers, Brian J" uniqKey="Eilers B" first="Brian J." last="Eilers">Brian J. Eilers</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Burns, Jennifer" sort="Burns, Jennifer" uniqKey="Burns J" first="Jennifer" last="Burns">Jennifer Burns</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Powell, Paul" sort="Powell, Paul" uniqKey="Powell P" first="Paul" last="Powell">Paul Powell</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fischer, Andreas M" sort="Fischer, Andreas M" uniqKey="Fischer A" first="Andreas M." last="Fischer">Andreas M. Fischer</name>
<affiliation wicri:level="1"><nlm:aff id="aff1a">Department of Plant Sciences and Plant Pathology,<institution>Montana State University</institution>
, Bozeman, Montana 59717,<country>United States</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Copie, Valerie" sort="Copie, Valerie" uniqKey="Copie V" first="Valérie" last="Copié">Valérie Copié</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
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<idno type="pmid">25495582</idno>
<idno type="pmc">4278681</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4278681</idno>
<idno type="RBID">PMC:4278681</idno>
<idno type="doi">10.1021/bi5007223</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000436</idno>
<idno type="wicri:Area/Pmc/Curation">000435</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000700</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structural and Biochemical Analysis of the <italic>Hordeum vulgare</italic>
L. <italic>Hv</italic>
GR-RBP1 Protein,
a Glycine-Rich RNA-Binding Protein Involved in the Regulation of Barley
Plant Development and Stress Response</title>
<author><name sortKey="Tripet, Brian X0a P" sort="Tripet, Brian X0a P" uniqKey="Tripet B" first="Brian X0a P." last="Tripet">Brian X0a P. Tripet</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mason, Katelyn E" sort="Mason, Katelyn E" uniqKey="Mason K" first="Katelyn E." last="Mason">Katelyn E. Mason</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Eilers, Brian J" sort="Eilers, Brian J" uniqKey="Eilers B" first="Brian J." last="Eilers">Brian J. Eilers</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Burns, Jennifer" sort="Burns, Jennifer" uniqKey="Burns J" first="Jennifer" last="Burns">Jennifer Burns</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Powell, Paul" sort="Powell, Paul" uniqKey="Powell P" first="Paul" last="Powell">Paul Powell</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fischer, Andreas M" sort="Fischer, Andreas M" uniqKey="Fischer A" first="Andreas M." last="Fischer">Andreas M. Fischer</name>
<affiliation wicri:level="1"><nlm:aff id="aff1a">Department of Plant Sciences and Plant Pathology,<institution>Montana State University</institution>
, Bozeman, Montana 59717,<country>United States</country>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Copie, Valerie" sort="Copie, Valerie" uniqKey="Copie V" first="Valérie" last="Copié">Valérie Copié</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff1a">Department of Chemistry and Biochemistry</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Biochemistry</title>
<idno type="ISSN">0006-2960</idno>
<idno type="eISSN">1520-4995</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en"><p content-type="toc-graphic"><graphic xlink:href="bi-2014-007223_0012" id="ab-tgr1"></graphic>
</p>
<p>The timing of whole-plant senescence
influences important agricultural
traits such as yield and grain protein content. Post-transcriptional
regulation by plant RNA-binding proteins is essential for proper control
of gene expression, development, and stress responses. Here, we report
the three-dimensional solution NMR structure and nucleic acid-binding
properties of the barley glycine-rich RNA-binding protein <italic>Hv</italic>
GR-RBP1, whose transcript has been identified as being
>45-fold up-regulated in early—as compared to late—senescing
near-isogenic barley germplasm. NMR analysis reveals that <italic>Hv</italic>
GR-RBP1 is a multidomain protein comprising a well-folded
N-terminal RNA Recognition Motif (RRM) and a structurally disordered
C-terminal glycine-rich domain. Chemical shift differences observed
in 2D <sup>1</sup>
H–<sup>15</sup>
N correlation (HSQC) NMR spectra
of full-length <italic>Hv</italic>
GR-RBP1 and N-<italic>Hv</italic>
GR-RBP1 (RRM domain only) suggest that the two domains can interact
both in-trans and intramolecularly, similar to what is observed in
the tobacco <italic>Nt</italic>
GR-RBP1 protein. Further, we show that
the RRM domain of <italic>Hv</italic>
GR-RBP1 binds single-stranded
DNA nucleotide fragments containing the consensus nucleotide sequence
5′-TTCTGX-3′ with low micromolar affinity <italic>in
vitro</italic>
. We also demonstrate that the C-terminal glycine-rich
(<italic>Hv</italic>
GR) domain of <italic>Hv</italic>
-GR-RBP1 can
interact nonspecifically with ssRNA <italic>in vitro</italic>
. Structural
similarities with other plant glycine-rich RNA-binding proteins suggest
that <italic>Hv</italic>
GR-RBP1 may be multifunctional. Based on gene
expression analysis following cold stress in barley and <italic>E. coli</italic>
growth studies following cold shock treatment,
we conclude that <italic>Hv</italic>
GR-RBP1 functions in a manner
similar to cold-shock proteins and harbors RNA chaperone activity. <italic>Hv</italic>
GR-RBP1 is therefore not only involved in the regulation
of barley development including senescence, but also functions in
plant responses to environmental stress.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zhang, H" uniqKey="Zhang H">H. Zhang</name>
</author>
<author><name sortKey="Zhou, C" uniqKey="Zhou C">C. Zhou</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Distelfeld, A" uniqKey="Distelfeld A">A. Distelfeld</name>
</author>
<author><name sortKey="Avni, R" uniqKey="Avni R">R. Avni</name>
</author>
<author><name sortKey="Fischer, A M" uniqKey="Fischer A">A. M. Fischer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="See, D" uniqKey="See D">D. See</name>
</author>
<author><name sortKey="Kanazin, V" uniqKey="Kanazin V">V. Kanazin</name>
</author>
<author><name sortKey="Kephart, K" uniqKey="Kephart K">K. Kephart</name>
</author>
<author><name sortKey="Black, T" uniqKey="Black T">T. Black</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gregersen, P L" uniqKey="Gregersen P">P. L. Gregersen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jukanti, A" uniqKey="Jukanti A">A. Jukanti</name>
</author>
<author><name sortKey="Heidlebaugh, N" uniqKey="Heidlebaugh N">N. Heidlebaugh</name>
</author>
<author><name sortKey="Parrott, D" uniqKey="Parrott D">D. Parrott</name>
</author>
<author><name sortKey="Fischer, I" uniqKey="Fischer I">I. Fischer</name>
</author>
<author><name sortKey="Mcinnerney, K" uniqKey="Mcinnerney K">K. McInnerney</name>
</author>
<author><name sortKey="Fischer, A" uniqKey="Fischer A">A. Fischer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jukanti, A K" uniqKey="Jukanti A">A. K. Jukanti</name>
</author>
<author><name sortKey="Fischer, A M" uniqKey="Fischer A">A. M. Fischer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Heidlebaugh, N M" uniqKey="Heidlebaugh N">N. M. Heidlebaugh</name>
</author>
<author><name sortKey="Trethewey, B R" uniqKey="Trethewey B">B. R. Trethewey</name>
</author>
<author><name sortKey="Jukanti, A K" uniqKey="Jukanti A">A. K. Jukanti</name>
</author>
<author><name sortKey="Parrott, D L" uniqKey="Parrott D">D. L. Parrott</name>
</author>
<author><name sortKey="Martin, J M" uniqKey="Martin J">J. M. Martin</name>
</author>
<author><name sortKey="Fischer, A M" uniqKey="Fischer A">A. M. Fischer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Streitner, C" uniqKey="Streitner C">C. Streitner</name>
</author>
<author><name sortKey="Danisman, S" uniqKey="Danisman S">S. Danisman</name>
</author>
<author><name sortKey="Wehrle, F" uniqKey="Wehrle F">F. Wehrle</name>
</author>
<author><name sortKey="Schoning, J C" uniqKey="Schoning J">J. C. Schoning</name>
</author>
<author><name sortKey="Alfano, J R" uniqKey="Alfano J">J. R. Alfano</name>
</author>
<author><name sortKey="Staiger, D" uniqKey="Staiger D">D. Staiger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gepstein, S" uniqKey="Gepstein S">S. Gepstein</name>
</author>
<author><name sortKey="Sabehi, G" uniqKey="Sabehi G">G. Sabehi</name>
</author>
<author><name sortKey="Carp, M J" uniqKey="Carp M">M.-J. Carp</name>
</author>
<author><name sortKey="Hajouj, T" uniqKey="Hajouj T">T. Hajouj</name>
</author>
<author><name sortKey="Nesher, M F O" uniqKey="Nesher M">M. F. O. Nesher</name>
</author>
<author><name sortKey="Yariv, I" uniqKey="Yariv I">I. Yariv</name>
</author>
<author><name sortKey="Dor, C" uniqKey="Dor C">C. Dor</name>
</author>
<author><name sortKey="Bassani, M" uniqKey="Bassani M">M. Bassani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lacerenza, J A" uniqKey="Lacerenza J">J. A. Lacerenza</name>
</author>
<author><name sortKey="Parrott, D L" uniqKey="Parrott D">D. L. Parrott</name>
</author>
<author><name sortKey="Fischer, A M" uniqKey="Fischer A">A. M. Fischer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parrott, D X0a L" uniqKey="Parrott D">D.
L. Parrott</name>
</author>
<author><name sortKey="Downs, E P" uniqKey="Downs E">E. P. Downs</name>
</author>
<author><name sortKey="Fischer, A M" uniqKey="Fischer A">A. M. Fischer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lorkovic, Z J" uniqKey="Lorkovic Z">Z. J. Lorkovic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mangeon, A" uniqKey="Mangeon A">A. Mangeon</name>
</author>
<author><name sortKey="Junqueira, R M" uniqKey="Junqueira R">R. M. Junqueira</name>
</author>
<author><name sortKey="Sachetto Martins, G" uniqKey="Sachetto Martins G">G. Sachetto-Martins</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sachetto Martins, G" uniqKey="Sachetto Martins G">G. Sachetto-Martins</name>
</author>
<author><name sortKey="Franco, L O" uniqKey="Franco L">L. O. Franco</name>
</author>
<author><name sortKey="De Oliveira, D E" uniqKey="De Oliveira D">D. E. de Oliveira</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alba, M M" uniqKey="Alba M">M. M. Alba</name>
</author>
<author><name sortKey="Pages, M" uniqKey="Pages M">M. Pages</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lorkovic, Z J" uniqKey="Lorkovic Z">Z. J. Lorkovic</name>
</author>
<author><name sortKey="Barta, A" uniqKey="Barta A">A. Barta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ambrosone, A" uniqKey="Ambrosone A">A. Ambrosone</name>
</author>
<author><name sortKey="Costa, A" uniqKey="Costa A">A. Costa</name>
</author>
<author><name sortKey="Leone, A" uniqKey="Leone A">A. Leone</name>
</author>
<author><name sortKey="Grillo, S" uniqKey="Grillo S">S. Grillo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kang, H" uniqKey="Kang H">H. Kang</name>
</author>
<author><name sortKey="Park, S J" uniqKey="Park S">S. J. Park</name>
</author>
<author><name sortKey="Kwak, K J" uniqKey="Kwak K">K. J. Kwak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woloshen, V" uniqKey="Woloshen V">V. Woloshen</name>
</author>
<author><name sortKey="Huang, S" uniqKey="Huang S">S. Huang</name>
</author>
<author><name sortKey="Li, X" uniqKey="Li X">X. Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Clery, A" uniqKey="Clery A">A. Clery</name>
</author>
<author><name sortKey="Blatter, M" uniqKey="Blatter M">M. Blatter</name>
</author>
<author><name sortKey="Allain, F" uniqKey="Allain F">F. Allain</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Daubner, G M" uniqKey="Daubner G">G. M. Daubner</name>
</author>
<author><name sortKey="Allain, F H" uniqKey="Allain F">F. H. Allain</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maris, C" uniqKey="Maris C">C. Maris</name>
</author>
<author><name sortKey="Dominguez, C" uniqKey="Dominguez C">C. Dominguez</name>
</author>
<author><name sortKey="Allain, F" uniqKey="Allain F">F. Allain</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sforca, M L" uniqKey="Sforca M">M. L. Sforca</name>
</author>
<author><name sortKey="Domingues, M N" uniqKey="Domingues M">M. N. Domingues</name>
</author>
<author><name sortKey="Zeri, A C M" uniqKey="Zeri A">A. C. M. Zeri</name>
</author>
<author><name sortKey="Benedetti, C E" uniqKey="Benedetti C">C. E. Benedetti</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Khan, F" uniqKey="Khan F">F. Khan</name>
</author>
<author><name sortKey="Daniels, M A" uniqKey="Daniels M">M. A. Daniels</name>
</author>
<author><name sortKey="Folkers, G E" uniqKey="Folkers G">G. E. Folkers</name>
</author>
<author><name sortKey="Boelens, R" uniqKey="Boelens R">R. Boelens</name>
</author>
<author><name sortKey="Naqci, S M S" uniqKey="Naqci S">S. M. S. Naqci</name>
</author>
<author><name sortKey="Van Ingen, H" uniqKey="Van Ingen H">H. van Ingen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schuttpelz, M" uniqKey="Schuttpelz M">M. Schuttpelz</name>
</author>
<author><name sortKey="Schoning, J C" uniqKey="Schoning J">J. C. Schoning</name>
</author>
<author><name sortKey="Doose, S" uniqKey="Doose S">S. Doose</name>
</author>
<author><name sortKey="Neuwiler, H" uniqKey="Neuwiler H">H. Neuwiler</name>
</author>
<author><name sortKey="Peters, E" uniqKey="Peters E">E. Peters</name>
</author>
<author><name sortKey="Staiger, D" uniqKey="Staiger D">D. Staiger</name>
</author>
<author><name sortKey="Sauer, M" uniqKey="Sauer M">M. Sauer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mason, K E" uniqKey="Mason K">K. E. Mason</name>
</author>
<author><name sortKey="Tripet, B P" uniqKey="Tripet B">B. P. Tripet</name>
</author>
<author><name sortKey="Parrott, D" uniqKey="Parrott D">D. Parrott</name>
</author>
<author><name sortKey="Fischer, A M" uniqKey="Fischer A">A. M. Fischer</name>
</author>
<author><name sortKey="Copie, V" uniqKey="Copie V">V. Copié</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Delaglio, F" uniqKey="Delaglio F">F. Delaglio</name>
</author>
<author><name sortKey="Grzesiek, S" uniqKey="Grzesiek S">S. Grzesiek</name>
</author>
<author><name sortKey="Vuister, G W" uniqKey="Vuister G">G. W. Vuister</name>
</author>
<author><name sortKey="Zhu, G" uniqKey="Zhu G">G. Zhu</name>
</author>
<author><name sortKey="Pfeifer, J" uniqKey="Pfeifer J">J. Pfeifer</name>
</author>
<author><name sortKey="Bax, A" uniqKey="Bax A">A. Bax</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goddard, T D" uniqKey="Goddard T">T. D. Goddard</name>
</author>
<author><name sortKey="Kneller, D G" uniqKey="Kneller D">D. G. Kneller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Shen, Y" uniqKey="Shen Y">Y. Shen</name>
</author>
<author><name sortKey="Delaglio, F" uniqKey="Delaglio F">F. Delaglio</name>
</author>
<author><name sortKey="Cornilescu, G" uniqKey="Cornilescu G">G. Cornilescu</name>
</author>
<author><name sortKey="A, B" uniqKey="A B">B. A</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Willard, L" uniqKey="Willard L">L. Willard</name>
</author>
<author><name sortKey="Ranjan, A" uniqKey="Ranjan A">A. Ranjan</name>
</author>
<author><name sortKey="Zhang, H" uniqKey="Zhang H">H. Zhang</name>
</author>
<author><name sortKey="Monzavi, H" uniqKey="Monzavi H">H. Monzavi</name>
</author>
<author><name sortKey="Boyko, R" uniqKey="Boyko R">R. Boyko</name>
</author>
<author><name sortKey="Sykes, B" uniqKey="Sykes B">B. Sykes</name>
</author>
<author><name sortKey="Wishart, D S" uniqKey="Wishart D">D. S. Wishart</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hwang, T" uniqKey="Hwang T">T. Hwang</name>
</author>
<author><name sortKey="Van Zill, P" uniqKey="Van Zill P">P. van Zill</name>
</author>
<author><name sortKey="Mori, S" uniqKey="Mori S">S. Mori</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guntert, P" uniqKey="Guntert P">P. Guntert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guntert, P" uniqKey="Guntert P">P. Guntert</name>
</author>
<author><name sortKey="Mumenthaler, C" uniqKey="Mumenthaler C">C. Mumenthaler</name>
</author>
<author><name sortKey="Wuthrich, K" uniqKey="Wuthrich K">K. Wuthrich</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Case, D A" uniqKey="Case D">D. A. Case</name>
</author>
<author><name sortKey="Darden, T A" uniqKey="Darden T">T. A. Darden</name>
</author>
<author><name sortKey="Cheatham, I" uniqKey="Cheatham I">I. Cheatham</name>
</author>
<author><name sortKey="T, E" uniqKey="T E">E. T</name>
</author>
<author><name sortKey="Simmerling, C L" uniqKey="Simmerling C">C. L. Simmerling</name>
</author>
<author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name>
</author>
<author><name sortKey="Duke, R X0a E" uniqKey="Duke R">R.
E. Duke</name>
</author>
<author><name sortKey="Luo, R" uniqKey="Luo R">R. Luo</name>
</author>
<author><name sortKey="Merz, K M" uniqKey="Merz K">K. M. Merz</name>
</author>
<author><name sortKey="Pearlman, D A" uniqKey="Pearlman D">D. A. Pearlman</name>
</author>
<author><name sortKey="Crowley, M" uniqKey="Crowley M">M. Crowley</name>
</author>
<author><name sortKey="Walker, R C" uniqKey="Walker R">R. C. Walker</name>
</author>
<author><name sortKey="Zhang, W" uniqKey="Zhang W">W. Zhang</name>
</author>
<author><name sortKey="Wang, B" uniqKey="Wang B">B. Wang</name>
</author>
<author><name sortKey="Hayik, S" uniqKey="Hayik S">S. Hayik</name>
</author>
<author><name sortKey="Roitberg, A" uniqKey="Roitberg A">A. Roitberg</name>
</author>
<author><name sortKey="Seabra, G" uniqKey="Seabra G">G. Seabra</name>
</author>
<author><name sortKey="Wong, K F" uniqKey="Wong K">K. F. Wong</name>
</author>
<author><name sortKey="Paesani, F" uniqKey="Paesani F">F. Paesani</name>
</author>
<author><name sortKey="Wu, X" uniqKey="Wu X">X. Wu</name>
</author>
<author><name sortKey="Brozell, S" uniqKey="Brozell S">S. Brozell</name>
</author>
<author><name sortKey="Tsui, V" uniqKey="Tsui V">V. Tsui</name>
</author>
<author><name sortKey="Gohlke, H" uniqKey="Gohlke H">H. Gohlke</name>
</author>
<author><name sortKey="Yang, L" uniqKey="Yang L">L. Yang</name>
</author>
<author><name sortKey="Tan, C" uniqKey="Tan C">C. Tan</name>
</author>
<author><name sortKey="Mongan, J" uniqKey="Mongan J">J. Mongan</name>
</author>
<author><name sortKey="Hornak, V" uniqKey="Hornak V">V. Hornak</name>
</author>
<author><name sortKey="Cui, G" uniqKey="Cui G">G. Cui</name>
</author>
<author><name sortKey="Beroza, P" uniqKey="Beroza P">P. Beroza</name>
</author>
<author><name sortKey="Mathews, D H" uniqKey="Mathews D">D. H. Mathews</name>
</author>
<author><name sortKey="Schafmeister, C" uniqKey="Schafmeister C">C. Schafmeister</name>
</author>
<author><name sortKey="Ross, W S" uniqKey="Ross W">W. S. Ross</name>
</author>
<author><name sortKey="Kollman, P A" uniqKey="Kollman P">P. A. Kollman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bertini, I" uniqKey="Bertini I">I. Bertini</name>
</author>
<author><name sortKey="Case, D A" uniqKey="Case D">D. A. Case</name>
</author>
<author><name sortKey="Ferella, L" uniqKey="Ferella L">L. Ferella</name>
</author>
<author><name sortKey="Giachetti, A" uniqKey="Giachetti A">A. Giachetti</name>
</author>
<author><name sortKey="Rosato, A" uniqKey="Rosato A">A. Rosato</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Laskowski, R A" uniqKey="Laskowski R">R. A. Laskowski</name>
</author>
<author><name sortKey="Rullmann, J A C" uniqKey="Rullmann J">J. A. C. Rullmann</name>
</author>
<author><name sortKey="Macarthur, M W" uniqKey="Macarthur M">M. W. MacArthur</name>
</author>
<author><name sortKey="Kaptein, R" uniqKey="Kaptein R">R. Kaptein</name>
</author>
<author><name sortKey="Thornton, J M" uniqKey="Thornton J">J. M. Thornton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, V B" uniqKey="Chen V">V. B. Chen</name>
</author>
<author><name sortKey="Arendall, W B" uniqKey="Arendall W">W. B. Arendall</name>
</author>
<author><name sortKey="Headd, J J" uniqKey="Headd J">J. J. Headd</name>
</author>
<author><name sortKey="Keedy, D A" uniqKey="Keedy D">D. A. Keedy</name>
</author>
<author><name sortKey="Immormino, R M" uniqKey="Immormino R">R. M. Immormino</name>
</author>
<author><name sortKey="Kapral, G J" uniqKey="Kapral G">G. J. Kapral</name>
</author>
<author><name sortKey="Murray, L W" uniqKey="Murray L">L. W. Murray</name>
</author>
<author><name sortKey="Richardson, J S" uniqKey="Richardson J">J. S. Richardson</name>
</author>
<author><name sortKey="Richardson, D C" uniqKey="Richardson D">D. C. Richardson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Luthy, R" uniqKey="Luthy R">R. Luthy</name>
</author>
<author><name sortKey="Bowie, J U" uniqKey="Bowie J">J. U. Bowie</name>
</author>
<author><name sortKey="Eisenberg, D" uniqKey="Eisenberg D">D. Eisenberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bhattacharya, A" uniqKey="Bhattacharya A">A. Bhattacharya</name>
</author>
<author><name sortKey="Tejreo, R" uniqKey="Tejreo R">R. Tejreo</name>
</author>
<author><name sortKey="Montelione, G T" uniqKey="Montelione G">G. T. Montelione</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Delano, W L" uniqKey="Delano W">W. L. DeLano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Holm, L" uniqKey="Holm L">L. Holm</name>
</author>
<author><name sortKey="Rosenstrom, P" uniqKey="Rosenstrom P">P. Rosenstrom</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Holm, L" uniqKey="Holm L">L. Holm</name>
</author>
<author><name sortKey="Kaariainen, S" uniqKey="Kaariainen S">S. Kaariainen</name>
</author>
<author><name sortKey="Rosenstrom, P" uniqKey="Rosenstrom P">P. Rosenstrom</name>
</author>
<author><name sortKey="Schenkel, A" uniqKey="Schenkel A">A. Schenkel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Johnson, M" uniqKey="Johnson M">M. Johnson</name>
</author>
<author><name sortKey="Zaretskaya, I" uniqKey="Zaretskaya I">I. Zaretskaya</name>
</author>
<author><name sortKey="Raytselis, Y" uniqKey="Raytselis Y">Y. Raytselis</name>
</author>
<author><name sortKey="Merezhuk, Y" uniqKey="Merezhuk Y">Y. Merezhuk</name>
</author>
<author><name sortKey="Mcginnis, S" uniqKey="Mcginnis S">S. McGinnis</name>
</author>
<author><name sortKey="Madden, T L" uniqKey="Madden T">T. L. Madden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Siever, F" uniqKey="Siever F">F. Siever</name>
</author>
<author><name sortKey="Wilm, A" uniqKey="Wilm A">A. Wilm</name>
</author>
<author><name sortKey="Dineen, D G" uniqKey="Dineen D">D. G. Dineen</name>
</author>
<author><name sortKey="Gibson, T J" uniqKey="Gibson T">T. J. Gibson</name>
</author>
<author><name sortKey="Karplus, K" uniqKey="Karplus K">K. Karplus</name>
</author>
<author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Lopez, R" uniqKey="Lopez R">R. Lopez</name>
</author>
<author><name sortKey="Mcwilliam, H" uniqKey="Mcwilliam H">H. McWilliam</name>
</author>
<author><name sortKey="Remmert, M" uniqKey="Remmert M">M. Remmert</name>
</author>
<author><name sortKey="Soding, J" uniqKey="Soding J">J. Söding</name>
</author>
<author><name sortKey="Thompson, J D" uniqKey="Thompson J">J. D. Thompson</name>
</author>
<author><name sortKey="Higgins, D G" uniqKey="Higgins D">D. G. Higgins</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smith, C W J" uniqKey="Smith C">C. W. J. Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bodenhausen, G" uniqKey="Bodenhausen G">G. Bodenhausen</name>
</author>
<author><name sortKey="Ruben, D J" uniqKey="Ruben D">D. J. Ruben</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, J Y" uniqKey="Kim J">J. Y. Kim</name>
</author>
<author><name sortKey="Kim, J S" uniqKey="Kim J">J. S. Kim</name>
</author>
<author><name sortKey="Kang, H" uniqKey="Kang H">H. Kang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, J Y" uniqKey="Kim J">J. Y. Kim</name>
</author>
<author><name sortKey="Park, S J" uniqKey="Park S">S. J. Park</name>
</author>
<author><name sortKey="Kwak, K J" uniqKey="Kwak K">K. J. Kwak</name>
</author>
<author><name sortKey="Kim, J O" uniqKey="Kim J">J. O. Kim</name>
</author>
<author><name sortKey="Kim, J Y" uniqKey="Kim J">J. Y. Kim</name>
</author>
<author><name sortKey="Song, J" uniqKey="Song J">J. Song</name>
</author>
<author><name sortKey="Jang, B" uniqKey="Jang B">B. Jang</name>
</author>
<author><name sortKey="Jung, C H" uniqKey="Jung C">C.-H. Jung</name>
</author>
<author><name sortKey="Kang, H" uniqKey="Kang H">H. Kang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, J Y" uniqKey="Kim J">J. Y. Kim</name>
</author>
<author><name sortKey="Kim, W Y" uniqKey="Kim W">W. Y. Kim</name>
</author>
<author><name sortKey="Kwak, K J" uniqKey="Kwak K">K. J. Kwak</name>
</author>
<author><name sortKey="Oh, S H" uniqKey="Oh S">S. H. Oh</name>
</author>
<author><name sortKey="Han, Y S" uniqKey="Han Y">Y. S. Han</name>
</author>
<author><name sortKey="Kang, H" uniqKey="Kang H">H. Kang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kwak, K J" uniqKey="Kwak K">K. J. Kwak</name>
</author>
<author><name sortKey="Park, S J" uniqKey="Park S">S. J. Park</name>
</author>
<author><name sortKey="Han, J H" uniqKey="Han J">J. H. Han</name>
</author>
<author><name sortKey="Kim, M K" uniqKey="Kim M">M. K. Kim</name>
</author>
<author><name sortKey="Oh, S H" uniqKey="Oh S">S. H. Oh</name>
</author>
<author><name sortKey="Han, Y S" uniqKey="Han Y">Y. S. Han</name>
</author>
<author><name sortKey="Kang, H" uniqKey="Kang H">H. Kang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dash, S" uniqKey="Dash S">S. Dash</name>
</author>
<author><name sortKey="Van Hemert, J" uniqKey="Van Hemert J">J. Van Hemert</name>
</author>
<author><name sortKey="Hong, L" uniqKey="Hong L">L. Hong</name>
</author>
<author><name sortKey="Wise, R P" uniqKey="Wise R">R. P. Wise</name>
</author>
<author><name sortKey="Dickerson, J A" uniqKey="Dickerson J">J. A. Dickerson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Close, T J" uniqKey="Close T">T. J. Close</name>
</author>
<author><name sortKey="Wanamaker, S I" uniqKey="Wanamaker S">S. I. Wanamaker</name>
</author>
<author><name sortKey="Caldo, R A" uniqKey="Caldo R">R. A. Caldo</name>
</author>
<author><name sortKey="Turner, S M" uniqKey="Turner S">S. M. Turner</name>
</author>
<author><name sortKey="Ashlock, D A" uniqKey="Ashlock D">D. A. Ashlock</name>
</author>
<author><name sortKey="Dickerson, J A" uniqKey="Dickerson J">J. A. Dickerson</name>
</author>
<author><name sortKey="Wing, R A" uniqKey="Wing R">R. A. Wing</name>
</author>
<author><name sortKey="Muehlbauer, G J" uniqKey="Muehlbauer G">G. J. Muehlbauer</name>
</author>
<author><name sortKey="Kleinhofs, A" uniqKey="Kleinhofs A">A. Kleinhofs</name>
</author>
<author><name sortKey="Wise, R P" uniqKey="Wise R">R. P. Wise</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Irizarry, R A" uniqKey="Irizarry R">R. A. Irizarry</name>
</author>
<author><name sortKey="Hobbs, B" uniqKey="Hobbs B">B. Hobbs</name>
</author>
<author><name sortKey="Collin, F" uniqKey="Collin F">F. Collin</name>
</author>
<author><name sortKey="Beazer Barclay, Y" uniqKey="Beazer Barclay Y">Y. Beazer-Barclay</name>
</author>
<author><name sortKey="Antonellis, K J" uniqKey="Antonellis K">K. J. Antonellis</name>
</author>
<author><name sortKey="Scherf, U" uniqKey="Scherf U">U. Scherf</name>
</author>
<author><name sortKey="Speed, T P" uniqKey="Speed T">T. P. Speed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Heintzen, C" uniqKey="Heintzen C">C. Heintzen</name>
</author>
<author><name sortKey="Nater, M" uniqKey="Nater M">M. Nater</name>
</author>
<author><name sortKey="Apel, K" uniqKey="Apel K">K. Apel</name>
</author>
<author><name sortKey="Staiger, D" uniqKey="Staiger D">D. Staiger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Carpenter, C D" uniqKey="Carpenter C">C. D. Carpenter</name>
</author>
<author><name sortKey="Kreps, J A" uniqKey="Kreps J">J. A. Kreps</name>
</author>
<author><name sortKey="Simon, A E" uniqKey="Simon A">A. E. Simon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Messais, A C" uniqKey="Messais A">A. C. Messais</name>
</author>
<author><name sortKey="Sattler, M" uniqKey="Sattler M">M. Sattler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Katahira, M" uniqKey="Katahira M">M. Katahira</name>
</author>
<author><name sortKey="Miyanoiri, Y" uniqKey="Miyanoiri Y">Y. Miyanoiri</name>
</author>
<author><name sortKey="Enokizono, Y" uniqKey="Enokizono Y">Y. Enokizono</name>
</author>
<author><name sortKey="Matsuda, G" uniqKey="Matsuda G">G. Matsuda</name>
</author>
<author><name sortKey="Nagata, T" uniqKey="Nagata T">T. Nagata</name>
</author>
<author><name sortKey="Ishikawa, F" uniqKey="Ishikawa F">F. Ishikawa</name>
</author>
<author><name sortKey="Uesugi, S" uniqKey="Uesugi S">S. Uesugi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Krissinel, E" uniqKey="Krissinel E">E. Krissinel</name>
</author>
<author><name sortKey="Henick, K" uniqKey="Henick K">K. Henick</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kumar, A O" uniqKey="Kumar A">A. O. Kumar</name>
</author>
<author><name sortKey="Swenson, M C" uniqKey="Swenson M">M. C. Swenson</name>
</author>
<author><name sortKey="Benning, M M" uniqKey="Benning M">M. M. Benning</name>
</author>
<author><name sortKey="Kielkopf, C L" uniqKey="Kielkopf C">C. L. Kielkopf</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Safaee, N" uniqKey="Safaee N">N. Safaee</name>
</author>
<author><name sortKey="Kozlov, G" uniqKey="Kozlov G">G. Kozlov</name>
</author>
<author><name sortKey="Noronha, A M" uniqKey="Noronha A">A. M. Noronha</name>
</author>
<author><name sortKey="Xie, J" uniqKey="Xie J">J. Xie</name>
</author>
<author><name sortKey="Wilds, C J" uniqKey="Wilds C">C. J. Wilds</name>
</author>
<author><name sortKey="Gehring, K B" uniqKey="Gehring K">K. B. Gehring</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Michel, Y M" uniqKey="Michel Y">Y. M. Michel</name>
</author>
<author><name sortKey="Poncet, D" uniqKey="Poncet D">D. Poncet</name>
</author>
<author><name sortKey="Piron, M" uniqKey="Piron M">M. Piron</name>
</author>
<author><name sortKey="Kean, K M" uniqKey="Kean K">K. M. Kean</name>
</author>
<author><name sortKey="Borman, A M" uniqKey="Borman A">A. M. Borman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Melamed, D" uniqKey="Melamed D">D. Melamed</name>
</author>
<author><name sortKey="Young, D L" uniqKey="Young D">D. L. Young</name>
</author>
<author><name sortKey="Gamble, C E" uniqKey="Gamble C">C. E. Gamble</name>
</author>
<author><name sortKey="Miller, C R" uniqKey="Miller C">C. R. Miller</name>
</author>
<author><name sortKey="Fields, S" uniqKey="Fields S">S. Fields</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Staiger, D" uniqKey="Staiger D">D. Staiger</name>
</author>
<author><name sortKey="Zecca, L" uniqKey="Zecca L">L. Zecca</name>
</author>
<author><name sortKey="Wieczorek, K D A" uniqKey="Wieczorek K">K. D. A. Wieczorek</name>
</author>
<author><name sortKey="Apel, K" uniqKey="Apel K">K. Apel</name>
</author>
<author><name sortKey="Eckstein, L" uniqKey="Eckstein L">L. Eckstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Streitner, C" uniqKey="Streitner C">C. Streitner</name>
</author>
<author><name sortKey="Koster, T" uniqKey="Koster T">T. Koster</name>
</author>
<author><name sortKey="Simpson, C G" uniqKey="Simpson C">C. G. Simpson</name>
</author>
<author><name sortKey="Shaw, P" uniqKey="Shaw P">P. Shaw</name>
</author>
<author><name sortKey="Danisman, S" uniqKey="Danisman S">S. Danisman</name>
</author>
<author><name sortKey="Brown, J W S" uniqKey="Brown J">J. W. S. Brown</name>
</author>
<author><name sortKey="Staiger, D" uniqKey="Staiger D">D. Staiger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schoning, J" uniqKey="Schoning J">J. Schoning</name>
</author>
<author><name sortKey="Streitner, C" uniqKey="Streitner C">C. Streitner</name>
</author>
<author><name sortKey="Page, D" uniqKey="Page D">D. Page</name>
</author>
<author><name sortKey="Hennig, S" uniqKey="Hennig S">S. Hennig</name>
</author>
<author><name sortKey="Uchida, K" uniqKey="Uchida K">K. Uchida</name>
</author>
<author><name sortKey="Wolf, E" uniqKey="Wolf E">E. Wolf</name>
</author>
<author><name sortKey="Furuya, M" uniqKey="Furuya M">M. Furuya</name>
</author>
<author><name sortKey="Staiger, D" uniqKey="Staiger D">D. Staiger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Volpon, L" uniqKey="Volpon L">L. Volpon</name>
</author>
<author><name sortKey="D Rsa, I" uniqKey="D Rsa I">I. D’Orsa</name>
</author>
<author><name sortKey="Young, C" uniqKey="Young C">C. Young</name>
</author>
<author><name sortKey="Frasch, A" uniqKey="Frasch A">A. Frasch</name>
</author>
<author><name sortKey="Gehring, K" uniqKey="Gehring K">K. Gehring</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Auweter, S" uniqKey="Auweter S">S. Auweter</name>
</author>
<author><name sortKey="Fasan, R" uniqKey="Fasan R">R. Fasan</name>
</author>
<author><name sortKey="Reymond, L" uniqKey="Reymond L">L. Reymond</name>
</author>
<author><name sortKey="Underwood, J" uniqKey="Underwood J">J. Underwood</name>
</author>
<author><name sortKey="Black, D" uniqKey="Black D">D. Black</name>
</author>
<author><name sortKey="Pitsch, S" uniqKey="Pitsch S">S. Pitsch</name>
</author>
<author><name sortKey="Allain, F" uniqKey="Allain F">F. Allain</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perez Canadillas, J" uniqKey="Perez Canadillas J">J. Perez-Canadillas</name>
</author>
<author><name sortKey="Varani, G" uniqKey="Varani G">G. Varani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fedoroff, N V" uniqKey="Fedoroff N">N. V. Fedoroff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Graumann, P L" uniqKey="Graumann P">P. L. Graumann</name>
</author>
<author><name sortKey="Marahiel, M A" uniqKey="Marahiel M">M. A. Marahiel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maruyama, K" uniqKey="Maruyama K">K. Maruyama</name>
</author>
<author><name sortKey="Sato, N" uniqKey="Sato N">N. Sato</name>
</author>
<author><name sortKey="Ohta, N" uniqKey="Ohta N">N. Ohta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nakaminami, K" uniqKey="Nakaminami K">K. Nakaminami</name>
</author>
<author><name sortKey="Karlson, D T" uniqKey="Karlson D">D. T. Karlson</name>
</author>
<author><name sortKey="Imai, R" uniqKey="Imai R">R. Imai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fu, Z" uniqKey="Fu Z">Z. Fu</name>
</author>
<author><name sortKey="Guo, M" uniqKey="Guo M">M. Guo</name>
</author>
<author><name sortKey="Jeong, B" uniqKey="Jeong B">B. Jeong</name>
</author>
<author><name sortKey="Tian, F" uniqKey="Tian F">F. Tian</name>
</author>
<author><name sortKey="Elthon, T" uniqKey="Elthon T">T. Elthon</name>
</author>
<author><name sortKey="Cerny, R" uniqKey="Cerny R">R. Cerny</name>
</author>
<author><name sortKey="Staiger, D" uniqKey="Staiger D">D. Staiger</name>
</author>
<author><name sortKey="A, A" uniqKey="A A">A. A</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article" xml:lang="EN"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biochemistry</journal-id>
<journal-id journal-id-type="iso-abbrev">Biochemistry</journal-id>
<journal-id journal-id-type="publisher-id">bi</journal-id>
<journal-id journal-id-type="coden">bichaw</journal-id>
<journal-title-group><journal-title>Biochemistry</journal-title>
</journal-title-group>
<issn pub-type="ppub">0006-2960</issn>
<issn pub-type="epub">1520-4995</issn>
<publisher><publisher-name>American
Chemical Society</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">25495582</article-id>
<article-id pub-id-type="pmc">4278681</article-id>
<article-id pub-id-type="doi">10.1021/bi5007223</article-id>
<article-categories><subj-group><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Structural and Biochemical Analysis of the <italic>Hordeum vulgare</italic>
L. <italic>Hv</italic>
GR-RBP1 Protein,
a Glycine-Rich RNA-Binding Protein Involved in the Regulation of Barley
Plant Development and Stress Response</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" id="ath1"><name><surname>Tripet</surname>
<given-names>Brian
P.</given-names>
</name>
<xref rid="aff1a" ref-type="aff">‡</xref>
</contrib>
<contrib contrib-type="author" id="ath2"><name><surname>Mason</surname>
<given-names>Katelyn E.</given-names>
</name>
<xref rid="aff1a" ref-type="aff">‡</xref>
</contrib>
<contrib contrib-type="author" id="ath3"><name><surname>Eilers</surname>
<given-names>Brian J.</given-names>
</name>
<xref rid="aff1a" ref-type="aff">‡</xref>
</contrib>
<contrib contrib-type="author" id="ath4"><name><surname>Burns</surname>
<given-names>Jennifer</given-names>
</name>
<xref rid="aff1a" ref-type="aff">‡</xref>
</contrib>
<contrib contrib-type="author" id="ath5"><name><surname>Powell</surname>
<given-names>Paul</given-names>
</name>
<xref rid="aff1a" ref-type="aff">‡</xref>
</contrib>
<contrib contrib-type="author" id="ath6"><name><surname>Fischer</surname>
<given-names>Andreas M.</given-names>
</name>
<xref rid="aff1a" ref-type="aff">§</xref>
</contrib>
<contrib contrib-type="author" corresp="yes" id="ath7"><name><surname>Copié</surname>
<given-names>Valérie</given-names>
</name>
<xref rid="cor1" ref-type="other">*</xref>
<xref rid="aff1a" ref-type="aff">‡</xref>
</contrib>
<aff id="aff1a"><sup>‡</sup>
Department of Chemistry and Biochemistry and<sup>§</sup>
Department of Plant Sciences and Plant Pathology,<institution>Montana State University</institution>
, Bozeman, Montana 59717,<country>United States</country>
</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>*</label>
E-mail: <email>vcopie@chemistry.montana.edu</email>
. Phone: <phone>(406) 994-7244</phone>
. Fax: <fax>(406) 994-5407</fax>
.</corresp>
</author-notes>
<pub-date pub-type="pmc-release"><day>25</day>
<month>11</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="epub"><day>25</day>
<month>11</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="ppub"><day>23</day>
<month>12</month>
<year>2014</year>
</pub-date>
<volume>53</volume>
<issue>50</issue>
<fpage>7945</fpage>
<lpage>7960</lpage>
<history><date date-type="received"><day>10</day>
<month>06</month>
<year>2014</year>
</date>
<date date-type="rev-recd"><day>25</day>
<month>11</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014 American Chemical
Society</copyright-statement>
<copyright-year>2014</copyright-year>
<copyright-holder>American Chemical
Society</copyright-holder>
<license><license-p>This is an open access article published under an ACS AuthorChoice <ext-link ext-link-type="uri" xlink:href="http://pubs.acs.org/page/policy/authorchoice_termsofuse.html">License</ext-link>
, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.</license-p>
</license>
</permissions>
<abstract><p content-type="toc-graphic"><graphic xlink:href="bi-2014-007223_0012" id="ab-tgr1"></graphic>
</p>
<p>The timing of whole-plant senescence
influences important agricultural
traits such as yield and grain protein content. Post-transcriptional
regulation by plant RNA-binding proteins is essential for proper control
of gene expression, development, and stress responses. Here, we report
the three-dimensional solution NMR structure and nucleic acid-binding
properties of the barley glycine-rich RNA-binding protein <italic>Hv</italic>
GR-RBP1, whose transcript has been identified as being
>45-fold up-regulated in early—as compared to late—senescing
near-isogenic barley germplasm. NMR analysis reveals that <italic>Hv</italic>
GR-RBP1 is a multidomain protein comprising a well-folded
N-terminal RNA Recognition Motif (RRM) and a structurally disordered
C-terminal glycine-rich domain. Chemical shift differences observed
in 2D <sup>1</sup>
H–<sup>15</sup>
N correlation (HSQC) NMR spectra
of full-length <italic>Hv</italic>
GR-RBP1 and N-<italic>Hv</italic>
GR-RBP1 (RRM domain only) suggest that the two domains can interact
both in-trans and intramolecularly, similar to what is observed in
the tobacco <italic>Nt</italic>
GR-RBP1 protein. Further, we show that
the RRM domain of <italic>Hv</italic>
GR-RBP1 binds single-stranded
DNA nucleotide fragments containing the consensus nucleotide sequence
5′-TTCTGX-3′ with low micromolar affinity <italic>in
vitro</italic>
. We also demonstrate that the C-terminal glycine-rich
(<italic>Hv</italic>
GR) domain of <italic>Hv</italic>
-GR-RBP1 can
interact nonspecifically with ssRNA <italic>in vitro</italic>
. Structural
similarities with other plant glycine-rich RNA-binding proteins suggest
that <italic>Hv</italic>
GR-RBP1 may be multifunctional. Based on gene
expression analysis following cold stress in barley and <italic>E. coli</italic>
growth studies following cold shock treatment,
we conclude that <italic>Hv</italic>
GR-RBP1 functions in a manner
similar to cold-shock proteins and harbors RNA chaperone activity. <italic>Hv</italic>
GR-RBP1 is therefore not only involved in the regulation
of barley development including senescence, but also functions in
plant responses to environmental stress.</p>
</abstract>
<funding-group><funding-statement><funding-source>National Institutes of Health, United States</funding-source>
</funding-statement>
</funding-group>
<custom-meta-group><custom-meta><meta-name>document-id-old-9</meta-name>
<meta-value>bi5007223</meta-value>
</custom-meta>
<custom-meta><meta-name>document-id-new-14</meta-name>
<meta-value>bi-2014-007223</meta-value>
</custom-meta>
<custom-meta><meta-name>ccc-price</meta-name>
<meta-value></meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Burns, Jennifer" sort="Burns, Jennifer" uniqKey="Burns J" first="Jennifer" last="Burns">Jennifer Burns</name>
<name sortKey="Copie, Valerie" sort="Copie, Valerie" uniqKey="Copie V" first="Valérie" last="Copié">Valérie Copié</name>
<name sortKey="Eilers, Brian J" sort="Eilers, Brian J" uniqKey="Eilers B" first="Brian J." last="Eilers">Brian J. Eilers</name>
<name sortKey="Mason, Katelyn E" sort="Mason, Katelyn E" uniqKey="Mason K" first="Katelyn E." last="Mason">Katelyn E. Mason</name>
<name sortKey="Powell, Paul" sort="Powell, Paul" uniqKey="Powell P" first="Paul" last="Powell">Paul Powell</name>
<name sortKey="Tripet, Brian X0a P" sort="Tripet, Brian X0a P" uniqKey="Tripet B" first="Brian X0a P." last="Tripet">Brian X0a P. Tripet</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Fischer, Andreas M" sort="Fischer, Andreas M" uniqKey="Fischer A" first="Andreas M." last="Fischer">Andreas M. Fischer</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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