Cryopreservation of Gemmae from the Liverwort Marchantia polymorpha L.
Identifieur interne : 000554 ( Pmc/Checkpoint ); précédent : 000553; suivant : 000555Cryopreservation of Gemmae from the Liverwort Marchantia polymorpha L.
Auteurs : Daisuke Tanaka [Japon] ; Kimitsune Ishizaki [Japon] ; Takayuki Kohchi [Japon] ; Katsuyuki T. Yamato [Japon]Source :
- Plant and Cell Physiology [ 0032-0781 ] ; 2015.
Abstract
The liverwort
Url:
DOI: 10.1093/pcp/pcv173
PubMed: 26561534
PubMed Central: 4788409
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cryopreservation of Gemmae from the Liverwort <italic>Marchantia polymorpha</italic> L.</title><author><name sortKey="Tanaka, Daisuke" sort="Tanaka, Daisuke" uniqKey="Tanaka D" first="Daisuke" last="Tanaka">Daisuke Tanaka</name><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF1">IBBP Center, National Institute for Basic Biology, Okazaki, 444-8787 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>IBBP Center, National Institute for Basic Biology, Okazaki</wicri:regionArea><wicri:noRegion>Okazaki</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF2">Department of Basic Biology, the Graduate University for Advanced Studies, Okazaki, 444-8585 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Basic Biology, the Graduate University for Advanced Studies, Okazaki</wicri:regionArea><wicri:noRegion>Okazaki</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF6">Present address: Genetic Resources Conservation Research Unit, Genetic Resources Center, National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Present address: Genetic Resources Conservation Research Unit, Genetic Resources Center, National Institute of Agrobiological Sciences, Tsukuba, 305-8602</wicri:regionArea><wicri:noRegion>305-8602</wicri:noRegion></affiliation></author><author><name sortKey="Ishizaki, Kimitsune" sort="Ishizaki, Kimitsune" uniqKey="Ishizaki K" first="Kimitsune" last="Ishizaki">Kimitsune Ishizaki</name><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF3">Graduate School of Science, Kobe University, Kobe, 657-8501 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Science, Kobe University, Kobe</wicri:regionArea><wicri:noRegion>Kobe</wicri:noRegion></affiliation></author><author><name sortKey="Kohchi, Takayuki" sort="Kohchi, Takayuki" uniqKey="Kohchi T" first="Takayuki" last="Kohchi">Takayuki Kohchi</name><affiliation wicri:level="4"><nlm:aff id="pcv173-AFF4">Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Biostudies, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Yamato, Katsuyuki T" sort="Yamato, Katsuyuki T" uniqKey="Yamato K" first="Katsuyuki T." last="Yamato">Katsuyuki T. Yamato</name><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF5">Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa, 649-6493 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa</wicri:regionArea><wicri:noRegion>Kinokawa</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26561534</idno><idno type="pmc">4788409</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4788409</idno><idno type="RBID">PMC:4788409</idno><idno type="doi">10.1093/pcp/pcv173</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000403</idno><idno type="wicri:Area/Pmc/Curation">000402</idno><idno type="wicri:Area/Pmc/Checkpoint">000554</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cryopreservation of Gemmae from the Liverwort <italic>Marchantia polymorpha</italic> L.</title><author><name sortKey="Tanaka, Daisuke" sort="Tanaka, Daisuke" uniqKey="Tanaka D" first="Daisuke" last="Tanaka">Daisuke Tanaka</name><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF1">IBBP Center, National Institute for Basic Biology, Okazaki, 444-8787 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>IBBP Center, National Institute for Basic Biology, Okazaki</wicri:regionArea><wicri:noRegion>Okazaki</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF2">Department of Basic Biology, the Graduate University for Advanced Studies, Okazaki, 444-8585 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Basic Biology, the Graduate University for Advanced Studies, Okazaki</wicri:regionArea><wicri:noRegion>Okazaki</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF6">Present address: Genetic Resources Conservation Research Unit, Genetic Resources Center, National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Japon</country><wicri:regionArea>Present address: Genetic Resources Conservation Research Unit, Genetic Resources Center, National Institute of Agrobiological Sciences, Tsukuba, 305-8602</wicri:regionArea><wicri:noRegion>305-8602</wicri:noRegion></affiliation></author><author><name sortKey="Ishizaki, Kimitsune" sort="Ishizaki, Kimitsune" uniqKey="Ishizaki K" first="Kimitsune" last="Ishizaki">Kimitsune Ishizaki</name><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF3">Graduate School of Science, Kobe University, Kobe, 657-8501 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Science, Kobe University, Kobe</wicri:regionArea><wicri:noRegion>Kobe</wicri:noRegion></affiliation></author><author><name sortKey="Kohchi, Takayuki" sort="Kohchi, Takayuki" uniqKey="Kohchi T" first="Takayuki" last="Kohchi">Takayuki Kohchi</name><affiliation wicri:level="4"><nlm:aff id="pcv173-AFF4">Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Graduate School of Biostudies, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Yamato, Katsuyuki T" sort="Yamato, Katsuyuki T" uniqKey="Yamato K" first="Katsuyuki T." last="Yamato">Katsuyuki T. Yamato</name><affiliation wicri:level="1"><nlm:aff id="pcv173-AFF5">Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa, 649-6493 Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa</wicri:regionArea><wicri:noRegion>Kinokawa</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant and Cell Physiology</title><idno type="ISSN">0032-0781</idno><idno type="eISSN">1471-9053</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The liverwort <italic>Marchantia polymorpha</italic> L. is one of the key model plants in evo–devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of <italic>M. polymorpha</italic> plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, thereby enabling the clonal preservation and rapid propagation of transgenic or mutant lines. Here, we report a simple cryopreservation protocol for in vitro grown <italic>M. polymorpha</italic> gemmae using aluminum cryoplates. Gemmae were pre-cultured on sucrose-containing medium, embedded in calcium alginate gel on the surface of a cryoplate, moderately dehydrated and stored in liquid nitrogen. After rapid thawing, the stored gemmae showed a 100% survival rate. Our protocol does not require plant growth regulators such as ABA, and takes only 1 h to complete except for 1 d of pre-culture. Furthermore, gemmae treated as described above but then air-dried for 2 h can be stored at −80°C for at least 1 year without a significant decrease in survival rate, which is convenient for most laboratories that have a −80°C freezer but not a liquid nitrogen container for long-term storage. These preservation techniques for <italic>M. polymorpha</italic> should increase their availability in the research community.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Baust, J G" uniqKey="Baust J">J.G. Baust</name></author><author><name sortKey="Gao, D Y" uniqKey="Gao D">D.Y. Gao</name></author><author><name sortKey="Baust, J M" uniqKey="Baust J">J.M. Baust</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bowman, J L" uniqKey="Bowman J">J.L. Bowman</name></author><author><name sortKey="Floyd, S K" uniqKey="Floyd S">S.K. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell Physiol</journal-id><journal-id journal-id-type="iso-abbrev">Plant Cell Physiol</journal-id><journal-id journal-id-type="publisher-id">pcp</journal-id><journal-id journal-id-type="hwp">pcellphys</journal-id><journal-title-group><journal-title>Plant and Cell Physiology</journal-title></journal-title-group><issn pub-type="ppub">0032-0781</issn><issn pub-type="epub">1471-9053</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26561534</article-id><article-id pub-id-type="pmc">4788409</article-id><article-id pub-id-type="doi">10.1093/pcp/pcv173</article-id><article-id pub-id-type="publisher-id">pcv173</article-id><article-categories><subj-group subj-group-type="heading"><subject>Special Focus Issue – Regular Papers</subject></subj-group></article-categories><title-group><article-title>Cryopreservation of Gemmae from the Liverwort <italic>Marchantia polymorpha</italic> L.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Tanaka</surname><given-names>Daisuke</given-names></name><xref ref-type="aff" rid="pcv173-AFF1"><sup>1</sup></xref><xref ref-type="aff" rid="pcv173-AFF2"><sup>2</sup></xref><xref ref-type="aff" rid="pcv173-AFF6"><sup>6</sup></xref><xref ref-type="corresp" rid="pcv173-COR1">*</xref></contrib><contrib contrib-type="author"><name><surname>Ishizaki</surname><given-names>Kimitsune</given-names></name><xref ref-type="aff" rid="pcv173-AFF3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kohchi</surname><given-names>Takayuki</given-names></name><xref ref-type="aff" rid="pcv173-AFF4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yamato</surname><given-names>Katsuyuki T.</given-names></name><xref ref-type="aff" rid="pcv173-AFF5"><sup>5</sup></xref></contrib><aff id="pcv173-AFF1"><sup>1</sup>IBBP Center, National Institute for Basic Biology, Okazaki, 444-8787 Japan</aff><aff id="pcv173-AFF2"><sup>2</sup>Department of Basic Biology, the Graduate University for Advanced Studies, Okazaki, 444-8585 Japan</aff><aff id="pcv173-AFF3"><sup>3</sup>Graduate School of Science, Kobe University, Kobe, 657-8501 Japan</aff><aff id="pcv173-AFF4"><sup>4</sup>Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502 Japan</aff><aff id="pcv173-AFF5"><sup>5</sup>Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa, 649-6493 Japan</aff><aff id="pcv173-AFF6"><sup>6</sup>Present address: Genetic Resources Conservation Research Unit, Genetic Resources Center, National Institute of Agrobiological Sciences, Tsukuba, 305-8602, Japan.</aff></contrib-group><author-notes><corresp id="pcv173-COR1">*Corresponding author: E-mail, <email>dtanaka@affrc.go.jp</email>; Fax, <fax>+81-29-838-7459</fax>.</corresp></author-notes><pub-date pub-type="ppub"><month>2</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>11</day><month>11</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>11</day><month>11</month><year>2015</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>57</volume><issue>2</issue><issue-title>Special Focus Issue Marchantia polymorpha</issue-title><fpage>300</fpage><lpage>306</lpage><history><date date-type="received"><day>13</day><month>3</month><year>2015</year></date><date date-type="accepted"><day>3</day><month>11</month><year>2015</year></date></history><permissions><copyright-statement>© The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. </copyright-statement><copyright-year>2015</copyright-year><license xlink:href="http://creativecommons.org/licenses/by-nc/4.0/" license-type="creative-commons"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/4.0/">http://creativecommons.org/licenses/by-nc/4.0/</ext-link>), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com</license-p></license></permissions><abstract><p>The liverwort <italic>Marchantia polymorpha</italic> L. is one of the key model plants in evo–devo studies, and an increasing number of transgenic and mutant lines have been established. For reliable long-term preservation of <italic>M. polymorpha</italic> plants, spores have been used, but crossing is indispensable to obtain them. Gemmae, however, are vegetative clones and readily available in large numbers without crossing, thereby enabling the clonal preservation and rapid propagation of transgenic or mutant lines. Here, we report a simple cryopreservation protocol for in vitro grown <italic>M. polymorpha</italic> gemmae using aluminum cryoplates. Gemmae were pre-cultured on sucrose-containing medium, embedded in calcium alginate gel on the surface of a cryoplate, moderately dehydrated and stored in liquid nitrogen. After rapid thawing, the stored gemmae showed a 100% survival rate. Our protocol does not require plant growth regulators such as ABA, and takes only 1 h to complete except for 1 d of pre-culture. Furthermore, gemmae treated as described above but then air-dried for 2 h can be stored at −80°C for at least 1 year without a significant decrease in survival rate, which is convenient for most laboratories that have a −80°C freezer but not a liquid nitrogen container for long-term storage. These preservation techniques for <italic>M. polymorpha</italic> should increase their availability in the research community.</p></abstract><kwd-group><kwd>Aluminum cryoplate</kwd><kwd>Bryophyte</kwd><kwd>Liquid nitrogen</kwd><kwd>Long-term preservation</kwd><kwd>Vitrification</kwd></kwd-group><counts><page-count count="7"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><noRegion><name sortKey="Tanaka, Daisuke" sort="Tanaka, Daisuke" uniqKey="Tanaka D" first="Daisuke" last="Tanaka">Daisuke Tanaka</name></noRegion><name sortKey="Ishizaki, Kimitsune" sort="Ishizaki, Kimitsune" uniqKey="Ishizaki K" first="Kimitsune" last="Ishizaki">Kimitsune Ishizaki</name><name sortKey="Kohchi, Takayuki" sort="Kohchi, Takayuki" uniqKey="Kohchi T" first="Takayuki" last="Kohchi">Takayuki Kohchi</name><name sortKey="Tanaka, Daisuke" sort="Tanaka, Daisuke" uniqKey="Tanaka D" first="Daisuke" last="Tanaka">Daisuke Tanaka</name><name sortKey="Tanaka, Daisuke" sort="Tanaka, Daisuke" uniqKey="Tanaka D" first="Daisuke" last="Tanaka">Daisuke Tanaka</name><name sortKey="Yamato, Katsuyuki T" sort="Yamato, Katsuyuki T" uniqKey="Yamato K" first="Katsuyuki T." last="Yamato">Katsuyuki T. Yamato</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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