Fragile Sites of ‘Valencia’ Sweet Orange (Citrus sinensis) Chromosomes Are Related with Active 45s rDNA
Identifieur interne : 000176 ( Pmc/Checkpoint ); précédent : 000175; suivant : 000177Fragile Sites of ‘Valencia’ Sweet Orange (Citrus sinensis) Chromosomes Are Related with Active 45s rDNA
Auteurs : Hong Lan [République populaire de Chine] ; Chun-Li Chen [République populaire de Chine] ; Yin Miao [République populaire de Chine] ; Chang-Xiu Yu [République populaire de Chine] ; Wen-Wu Guo [République populaire de Chine] ; Qiang Xu [République populaire de Chine] ; Xiu-Xin Deng [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
Url:
DOI: 10.1371/journal.pone.0151512
PubMed: 26977938
PubMed Central: 4792391
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fragile Sites of ‘Valencia’ Sweet Orange (<italic>Citrus sinensis</italic>) Chromosomes Are Related with Active 45s rDNA</title><author><name sortKey="Lan, Hong" sort="Lan, Hong" uniqKey="Lan H" first="Hong" last="Lan">Hong Lan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Chun Li" sort="Chen, Chun Li" uniqKey="Chen C" first="Chun-Li" last="Chen">Chun-Li Chen</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Miao, Yin" sort="Miao, Yin" uniqKey="Miao Y" first="Yin" last="Miao">Yin Miao</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Chang Xiu" sort="Yu, Chang Xiu" uniqKey="Yu C" first="Chang-Xiu" last="Yu">Chang-Xiu Yu</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Wen Wu" sort="Guo, Wen Wu" uniqKey="Guo W" first="Wen-Wu" last="Guo">Wen-Wu Guo</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Xiu Xin" sort="Deng, Xiu Xin" uniqKey="Deng X" first="Xiu-Xin" last="Deng">Xiu-Xin Deng</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26977938</idno><idno type="pmc">4792391</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792391</idno><idno type="RBID">PMC:4792391</idno><idno type="doi">10.1371/journal.pone.0151512</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000382</idno><idno type="wicri:Area/Pmc/Curation">000381</idno><idno type="wicri:Area/Pmc/Checkpoint">000176</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Fragile Sites of ‘Valencia’ Sweet Orange (<italic>Citrus sinensis</italic>) Chromosomes Are Related with Active 45s rDNA</title><author><name sortKey="Lan, Hong" sort="Lan, Hong" uniqKey="Lan H" first="Hong" last="Lan">Hong Lan</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Chun Li" sort="Chen, Chun Li" uniqKey="Chen C" first="Chun-Li" last="Chen">Chun-Li Chen</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Miao, Yin" sort="Miao, Yin" uniqKey="Miao Y" first="Yin" last="Miao">Yin Miao</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Chang Xiu" sort="Yu, Chang Xiu" uniqKey="Yu C" first="Chang-Xiu" last="Yu">Chang-Xiu Yu</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Wen Wu" sort="Guo, Wen Wu" uniqKey="Guo W" first="Wen-Wu" last="Guo">Wen-Wu Guo</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Xiu Xin" sort="Deng, Xiu Xin" uniqKey="Deng X" first="Xiu-Xin" last="Deng">Xiu-Xin Deng</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070</wicri:regionArea><wicri:noRegion>430070</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Citrus sinensis</italic> chromosomes present a morphological differentiation of bands after staining by the fluorochromes CMA and DAPI, but there is still little information on its chromosomal characteristics. In this study, the chromosomes in ‘Valencia’ <italic>C</italic>. <italic>sinensis</italic> were analyzed by fluorescence <italic>in situ</italic> hybridization (FISH) using telomere DNA and the 45S rDNA gene as probes combining CMA/DAPI staining, which showed that there were two fragile sites in sweet orange chromosomes co-localizing at distended 45S rDNA regions, one proximally locating on B-type chromosome and the other subterminally locating on D-type chromosome. While the chromosomal CMA banding and 45S rDNA FISH mapping in the doubled haploid line of ‘Valencia’ <italic>C</italic>. <italic>sinensis</italic> indicated six 45S rDNA regions, four were identified as fragile sites as doubled comparing its parental line, which confirmed the cytological heterozygosity and chromosomal heteromorphisms in sweet orange. Furthermore, Ag-NOR identified two distended 45S rDNA regions to be active nucleolar organizing regions (NORs) in diploid ‘Valencia’ <italic>C</italic>. <italic>sinensis</italic>. The occurrence of quadrivalent in meiosis of pollen mother cells (PMCs) in ‘Valencia’ sweet orange further confirmed it was a chromosomal reciprocal translocation line. We speculated this chromosome translocation was probably related to fragile sites. Our data provide insights into the chromosomal characteristics of the fragile sites in ‘Valencia’ sweet orange and are expected to facilitate the further investigation of the possible functions of fragile sites.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name></author><author><name sortKey="Gmitter, Fg" uniqKey="Gmitter F">FG Gmitter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Q" uniqKey="Xu Q">Q Xu</name></author><author><name sortKey="Chen, Ll" uniqKey="Chen L">LL Chen</name></author><author><name sortKey="Ruan, X" uniqKey="Ruan X">X Ruan</name></author><author><name sortKey="Chen, D" uniqKey="Chen D">D Chen</name></author><author><name sortKey="Zhu, A" uniqKey="Zhu A">A Zhu</name></author><author><name sortKey="Chen, Cl" uniqKey="Chen C">CL Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scora, Rw" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, CA USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26977938</article-id><article-id pub-id-type="pmc">4792391</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0151512</article-id><article-id pub-id-type="publisher-id">PONE-D-15-52901</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Chromosome Biology</subject><subj-group><subject>Chromosomes</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Cell Processes</subject><subj-group><subject>Cell Cycle and Cell Division</subject><subj-group><subject>Metaphase</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Behavior</subject><subj-group><subject>Animal Behavior</subject><subj-group><subject>Animal Signaling and Communication</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Zoology</subject><subj-group><subject>Animal Behavior</subject><subj-group><subject>Animal Signaling and Communication</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Cell Biology</subject><subj-group><subject>Chromosome Biology</subject><subj-group><subject>Chromosomes</subject><subj-group><subject>Homologous Chromosomes</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Specimen Preparation and Treatment</subject><subj-group><subject>Staining</subject><subj-group><subject>Chromosome Staining</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Molecular Biology</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Gene Mapping</subject><subj-group><subject>Chromosome Mapping</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Gene Mapping</subject><subj-group><subject>Chromosome Mapping</subject></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Cytogenetic Techniques</subject><subj-group><subject>Fluorescent in Situ Hybridization</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Molecular Biology</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Molecular Probe Techniques</subject><subj-group><subject>Probe Hybridization</subject><subj-group><subject>Fluorescent in Situ Hybridization</subject></subj-group></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Research and Analysis Methods</subject><subj-group><subject>Molecular Biology Techniques</subject><subj-group><subject>Molecular Probe Techniques</subject><subj-group><subject>Probe Hybridization</subject><subj-group><subject>Fluorescent in Situ Hybridization</subject></subj-group></subj-group></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v3"><subject>Biology and Life Sciences</subject><subj-group><subject>Genetics</subject><subj-group><subject>Cytogenetics</subject><subj-group><subject>Karyotypes</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Fragile Sites of ‘Valencia’ Sweet Orange (<italic>Citrus sinensis</italic>) Chromosomes Are Related with Active 45s rDNA</article-title><alt-title alt-title-type="running-head">Chromosomal Characteristic with Fragile Sites in ‘Valencia’ Sweet Orange (<italic>Citrus sinensis</italic>)</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lan</surname><given-names>Hong</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Chun-Li</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib><contrib contrib-type="author"><name><surname>Miao</surname><given-names>Yin</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yu</surname><given-names>Chang-Xiu</given-names></name><xref ref-type="aff" rid="aff002"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Guo</surname><given-names>Wen-Wu</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Qiang</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Deng</surname><given-names>Xiu-Xin</given-names></name><xref ref-type="aff" rid="aff001"><sup>1</sup></xref><xref ref-type="corresp" rid="cor001">*</xref></contrib></contrib-group><aff id="aff001"><label>1</label><addr-line>Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China</addr-line></aff><aff id="aff002"><label>2</label><addr-line>College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Li</surname><given-names>Maoteng</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>Huazhong university of Science and Technology, CHINA</addr-line></aff><author-notes><fn fn-type="conflict" id="coi001"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con" id="contrib001"><p>Conceived and designed the experiments: CC XD. Performed the experiments: HL YM. Analyzed the data: CC HL YM CY WG QX XD. Contributed reagents/materials/analysis tools: CC WG QX XD. Wrote the paper: CC XD HL.</p></fn><corresp id="cor001">* E-mail: <email>chenchunli@mail.hzau.edu.cn</email> (CC); <email>xxdeng@mail.hzau.edu.cn</email> (XD)</corresp></author-notes><pub-date pub-type="epub"><day>15</day><month>3</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>11</volume><issue>3</issue><elocation-id>e0151512</elocation-id><history><date date-type="received"><day>31</day><month>12</month><year>2015</year></date><date date-type="accepted"><day>29</day><month>2</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 Lan et al</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>Lan et al</copyright-holder><license xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an open access article distributed under the terms of the <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</ext-link>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="pone.0151512.pdf"></self-uri><abstract><p><italic>Citrus sinensis</italic> chromosomes present a morphological differentiation of bands after staining by the fluorochromes CMA and DAPI, but there is still little information on its chromosomal characteristics. In this study, the chromosomes in ‘Valencia’ <italic>C</italic>. <italic>sinensis</italic> were analyzed by fluorescence <italic>in situ</italic> hybridization (FISH) using telomere DNA and the 45S rDNA gene as probes combining CMA/DAPI staining, which showed that there were two fragile sites in sweet orange chromosomes co-localizing at distended 45S rDNA regions, one proximally locating on B-type chromosome and the other subterminally locating on D-type chromosome. While the chromosomal CMA banding and 45S rDNA FISH mapping in the doubled haploid line of ‘Valencia’ <italic>C</italic>. <italic>sinensis</italic> indicated six 45S rDNA regions, four were identified as fragile sites as doubled comparing its parental line, which confirmed the cytological heterozygosity and chromosomal heteromorphisms in sweet orange. Furthermore, Ag-NOR identified two distended 45S rDNA regions to be active nucleolar organizing regions (NORs) in diploid ‘Valencia’ <italic>C</italic>. <italic>sinensis</italic>. The occurrence of quadrivalent in meiosis of pollen mother cells (PMCs) in ‘Valencia’ sweet orange further confirmed it was a chromosomal reciprocal translocation line. We speculated this chromosome translocation was probably related to fragile sites. Our data provide insights into the chromosomal characteristics of the fragile sites in ‘Valencia’ sweet orange and are expected to facilitate the further investigation of the possible functions of fragile sites.</p></abstract><funding-group><funding-statement>This research was supported by the National Natural Science Foundation of China (Nos. 31330066, 31221062) to X. Deng, and the Fundamental Research Funds for the Central Universities (2013PY084) to C. Chen.</funding-statement></funding-group><counts><fig-count count="6"></fig-count><table-count count="0"></table-count><page-count count="15"></page-count></counts><custom-meta-group><custom-meta id="data-availability"><meta-name>Data Availability</meta-name><meta-value>All relevant data are within the paper.</meta-value></custom-meta></custom-meta-group></article-meta><notes><title>Data Availability</title><p>All relevant data are within the paper.</p></notes></front></pmc><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lan, Hong" sort="Lan, Hong" uniqKey="Lan H" first="Hong" last="Lan">Hong Lan</name></noRegion><name sortKey="Chen, Chun Li" sort="Chen, Chun Li" uniqKey="Chen C" first="Chun-Li" last="Chen">Chun-Li Chen</name><name sortKey="Deng, Xiu Xin" sort="Deng, Xiu Xin" uniqKey="Deng X" first="Xiu-Xin" last="Deng">Xiu-Xin Deng</name><name sortKey="Guo, Wen Wu" sort="Guo, Wen Wu" uniqKey="Guo W" first="Wen-Wu" last="Guo">Wen-Wu Guo</name><name sortKey="Miao, Yin" sort="Miao, Yin" uniqKey="Miao Y" first="Yin" last="Miao">Yin Miao</name><name sortKey="Xu, Qiang" sort="Xu, Qiang" uniqKey="Xu Q" first="Qiang" last="Xu">Qiang Xu</name><name sortKey="Yu, Chang Xiu" sort="Yu, Chang Xiu" uniqKey="Yu C" first="Chang-Xiu" last="Yu">Chang-Xiu Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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