Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line
Identifieur interne : 000053 ( Pmc/Checkpoint ); précédent : 000052; suivant : 000054Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line
Auteurs : Wenjing Xu [République populaire de Chine] ; Hongjun Lv [République populaire de Chine] ; Mingming Zhao [République populaire de Chine] ; Yongchao Li [République populaire de Chine] ; Yueying Qi [République populaire de Chine] ; Zhenying Peng [République populaire de Chine] ; Guangmin Xia [République populaire de Chine] ; Mengcheng Wang [République populaire de Chine]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
We previously bred a salt tolerant wheat cv. SR3 with bread wheat cv. JN177 as the parent via asymmetric somatic hybridization, and found that the tolerance is partially attributed to the superior photosynthesis capacity. Here, we compared the proteomes of two cultivars to unravel the basis of superior photosynthesis capacity. In the maps of two dimensional difference gel electrophoresis (2D-DIGE), there were 26 differentially expressed proteins (DEPs), including 18 cultivar-based and 8 stress-responsive ones. 21 of 26 DEPs were identified and classified into four categories, including photosynthesis, photosynthesis system stability, linolenic acid metabolism, and protein synthesis in chloroplast. The chloroplast localization of some DEPs confirmed that the identified DEPs function in the chloroplast. The overexpression of a DEP enhanced salt tolerance in
Url:
DOI: 10.1038/srep32384
PubMed: 27562633
PubMed Central: 4999883
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4999883Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line</title><author><name sortKey="Xu, Wenjing" sort="Xu, Wenjing" uniqKey="Xu W" first="Wenjing" last="Xu">Wenjing Xu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lv, Hongjun" sort="Lv, Hongjun" uniqKey="Lv H" first="Hongjun" last="Lv">Hongjun Lv</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Mingming" sort="Zhao, Mingming" uniqKey="Zhao M" first="Mingming" last="Zhao">Mingming Zhao</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Yongchao" sort="Li, Yongchao" uniqKey="Li Y" first="Yongchao" last="Li">Yongchao Li</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Qi, Yueying" sort="Qi, Yueying" uniqKey="Qi Y" first="Yueying" last="Qi">Yueying Qi</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Peng, Zhenying" sort="Peng, Zhenying" uniqKey="Peng Z" first="Zhenying" last="Peng">Zhenying Peng</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Bio-Tech Research Center, Shandong Academy of Agricultural Science, Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crop</institution>, Jinan, 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Mengcheng" sort="Wang, Mengcheng" uniqKey="Wang M" first="Mengcheng" last="Wang">Mengcheng Wang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27562633</idno><idno type="pmc">4999883</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999883</idno><idno type="RBID">PMC:4999883</idno><idno type="doi">10.1038/srep32384</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000475</idno><idno type="wicri:Area/Pmc/Curation">000474</idno><idno type="wicri:Area/Pmc/Checkpoint">000053</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line</title><author><name sortKey="Xu, Wenjing" sort="Xu, Wenjing" uniqKey="Xu W" first="Wenjing" last="Xu">Wenjing Xu</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lv, Hongjun" sort="Lv, Hongjun" uniqKey="Lv H" first="Hongjun" last="Lv">Hongjun Lv</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhao, Mingming" sort="Zhao, Mingming" uniqKey="Zhao M" first="Mingming" last="Zhao">Mingming Zhao</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Yongchao" sort="Li, Yongchao" uniqKey="Li Y" first="Yongchao" last="Li">Yongchao Li</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Qi, Yueying" sort="Qi, Yueying" uniqKey="Qi Y" first="Yueying" last="Qi">Yueying Qi</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Peng, Zhenying" sort="Peng, Zhenying" uniqKey="Peng Z" first="Zhenying" last="Peng">Zhenying Peng</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Bio-Tech Research Center, Shandong Academy of Agricultural Science, Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crop</institution>, Jinan, 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Mengcheng" sort="Wang, Mengcheng" uniqKey="Wang M" first="Mengcheng" last="Wang">Mengcheng Wang</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We previously bred a salt tolerant wheat cv. SR3 with bread wheat cv. JN177 as the parent via asymmetric somatic hybridization, and found that the tolerance is partially attributed to the superior photosynthesis capacity. Here, we compared the proteomes of two cultivars to unravel the basis of superior photosynthesis capacity. In the maps of two dimensional difference gel electrophoresis (2D-DIGE), there were 26 differentially expressed proteins (DEPs), including 18 cultivar-based and 8 stress-responsive ones. 21 of 26 DEPs were identified and classified into four categories, including photosynthesis, photosynthesis system stability, linolenic acid metabolism, and protein synthesis in chloroplast. The chloroplast localization of some DEPs confirmed that the identified DEPs function in the chloroplast. The overexpression of a DEP enhanced salt tolerance in <italic>Arabidopsis thaliana</italic>. In line with these data, it is concluded that the contribution of chloroplast to high salinity tolerance of wheat cv. SR3 appears to include higher photosynthesis efficiency by promoting system protection and ROS clearance, stronger production of phytohormone JA by enhancing metabolism activity, and modulating the <italic>in chloroplast</italic> synthesis of proteins.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Pogson, B J" uniqKey="Pogson B">B. J. Pogson</name></author><author><name sortKey="Woo, N S" uniqKey="Woo N">N. S. Woo</name></author><author><name sortKey="Forster, B" uniqKey="Forster B">B. Forster</name></author><author><name sortKey="Small, I D" uniqKey="Small I">I. D. Small</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woodson, J D" uniqKey="Woodson J">J. D. Woodson</name></author><author><name sortKey="Chory, J" uniqKey="Chory J">J. Chory</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ng, S" uniqKey="Ng S">S. Ng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Asada, K" uniqKey="Asada K">K. Asada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Galvez Valdivieso, G" uniqKey="Galvez Valdivieso G">G. Galvez-Valdivieso</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fryer, M" uniqKey="Fryer M">M. Fryer</name></author><author><name sortKey="Oxborough, K" uniqKey="Oxborough K">K. Oxborough</name></author><author><name sortKey="Mullineaux, P M" uniqKey="Mullineaux P">P. M. Mullineaux</name></author><author><name sortKey="Baker, N R" uniqKey="Baker N">N. R. Baker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krieger Liszkay, A" uniqKey="Krieger Liszkay A">A. Krieger-Liszkay</name></author><author><name sortKey="Fufezan, C" uniqKey="Fufezan C">C. Fufezan</name></author><author><name sortKey="Trebst, A" uniqKey="Trebst A">A. Trebst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Foyer, C H" uniqKey="Foyer C">C. H. Foyer</name></author><author><name sortKey="Noctor, G" uniqKey="Noctor G">G. Noctor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Allen, J F" uniqKey="Allen J">J. F. Allen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pfannschmidt, T" uniqKey="Pfannschmidt T">T. Pfannschmidt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munns, R" uniqKey="Munns R">R. Munns</name></author><author><name sortKey="Tester, M" uniqKey="Tester M">M. Tester</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xia, G M" uniqKey="Xia G">G. M. Xia</name></author><author><name sortKey="Xiang, F N" uniqKey="Xiang F">F. N. Xiang</name></author><author><name sortKey="Zhou, A F" uniqKey="Zhou A">A. F. Zhou</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Chen, H M" uniqKey="Chen H">H. M. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author><author><name sortKey="Xiang, F N" uniqKey="Xiang F">F. N. Xiang</name></author><author><name sortKey="Xia, G M" uniqKey="Xia G">G. M. Xia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, S" uniqKey="Liu S">S. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, M" uniqKey="Wang M">M. Wang</name></author><author><name sortKey="Liu, C" uniqKey="Liu C">C. Liu</name></author><author><name sortKey="Xing, T" uniqKey="Xing T">T. Xing</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Xia, G" uniqKey="Xia G">G. Xia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, C" uniqKey="Li C">C. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, S" uniqKey="Liu S">S. Liu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, S" uniqKey="Liu S">S. Liu</name></author><author><name sortKey="Xia, G" uniqKey="Xia G">G. Xia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, C" uniqKey="Liu C">C. Liu</name></author><author><name sortKey="Li, S" uniqKey="Li S">S. Li</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M. Wang</name></author><author><name sortKey="Xia, G" uniqKey="Xia G">G. Xia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peng, Z" uniqKey="Peng Z">Z. Peng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, M C" uniqKey="Wang M">M.-C. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, S Y" uniqKey="Chen S">S. Y. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, A F" uniqKey="Zhou A">A. F. Zhou</name></author><author><name sortKey="Xia, G M" uniqKey="Xia G">G. M. Xia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, A X" uniqKey="Cheng A">A. X. Cheng</name></author><author><name sortKey="Xia, G M" uniqKey="Xia G">G. M. Xia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xiang, F N" uniqKey="Xiang F">F. N. Xiang</name></author><author><name sortKey="Xia, G M" uniqKey="Xia G">G. M. Xia</name></author><author><name sortKey="Chen, H M" uniqKey="Chen H">H. M. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, S Y" uniqKey="Lee S">S. Y. Lee</name></author><author><name sortKey="Damodaran, P N" uniqKey="Damodaran P">P. N. Damodaran</name></author><author><name sortKey="Roh, K S" uniqKey="Roh K">K. S. Roh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hurwitz, J" uniqKey="Hurwitz J">J. Hurwitz</name></author><author><name sortKey="Weissbach, A" uniqKey="Weissbach A">A. Weissbach</name></author><author><name sortKey="Horecker, B L" uniqKey="Horecker B">B. L. Horecker</name></author><author><name sortKey="Smyrniotis, P Z" uniqKey="Smyrniotis P">P. Z. Smyrniotis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bradford, M" uniqKey="Bradford M">M. Bradford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ellis, M H" uniqKey="Ellis M">M. H. Ellis</name></author><author><name sortKey="Setter, T L" uniqKey="Setter T">T. L. Setter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrook, J" uniqKey="Sambrook J">J. Sambrook</name></author><author><name sortKey="Fritsch, E F" uniqKey="Fritsch E">E. F. Fritsch</name></author><author><name sortKey="Maniatis, T" uniqKey="Maniatis T">T. Maniatis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Livak, K J" uniqKey="Livak K">K. J. Livak</name></author><author><name sortKey="Schmittgen, T D" uniqKey="Schmittgen T">T. D. Schmittgen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, X" uniqKey="Huang X">X. Huang</name></author><author><name sortKey="Madan, A" uniqKey="Madan A">A. Madan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoo, S D" uniqKey="Yoo S">S. D. Yoo</name></author><author><name sortKey="Cho, Y H" uniqKey="Cho Y">Y. H. Cho</name></author><author><name sortKey="Sheen, J" uniqKey="Sheen J">J. Sheen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clough, S J" uniqKey="Clough S">S. J. Clough</name></author><author><name sortKey="Bent, A F" uniqKey="Bent A">A. F. Bent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Voss, I" uniqKey="Voss I">I. Voss</name></author><author><name sortKey="Sunil, B" uniqKey="Sunil B">B. Sunil</name></author><author><name sortKey="Scheibe, R" uniqKey="Scheibe R">R. Scheibe</name></author><author><name sortKey="Raghavendra, A S" uniqKey="Raghavendra A">A. S. Raghavendra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Houtz, R L" uniqKey="Houtz R">R. L. Houtz</name></author><author><name sortKey="Poneleit, L" uniqKey="Poneleit L">L. Poneleit</name></author><author><name sortKey="Jones, S B" uniqKey="Jones S">S. B. Jones</name></author><author><name sortKey="Royer, M" uniqKey="Royer M">M. Royer</name></author><author><name sortKey="Stults, J T" uniqKey="Stults J">J. T. Stults</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Houtz, R L" uniqKey="Houtz R">R. L. Houtz</name></author><author><name sortKey="Stults, J T" uniqKey="Stults J">J. T. Stults</name></author><author><name sortKey="Mulligan, R M" uniqKey="Mulligan R">R. M. Mulligan</name></author><author><name sortKey="Tolbert, N E" uniqKey="Tolbert N">N. E. Tolbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mano, J I" uniqKey="Mano J">J. I. Mano</name></author><author><name sortKey="Nagata, M" uniqKey="Nagata M">M. Nagata</name></author><author><name sortKey="Okamura, S" uniqKey="Okamura S">S. Okamura</name></author><author><name sortKey="Shiraya, T" uniqKey="Shiraya T">T. Shiraya</name></author><author><name sortKey="Mitsui, T" uniqKey="Mitsui T">T. Mitsui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Porter, M A" uniqKey="Porter M">M. A. Porter</name></author><author><name sortKey="Stringer, C D" uniqKey="Stringer C">C. D. Stringer</name></author><author><name sortKey="Hartman, F C" uniqKey="Hartman F">F. C. Hartman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kosova, K" uniqKey="Kosova K">K. Kosová</name></author><author><name sortKey="Prasil, I T" uniqKey="Prasil I">I. T. Prášil</name></author><author><name sortKey="Vitamvas, P" uniqKey="Vitamvas P">P. Vítámvás</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Z J" uniqKey="Chen Z">Z. J. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, M" uniqKey="Wang M">M. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Plucken, H" uniqKey="Plucken H">H. Plücken</name></author><author><name sortKey="Muller, B" uniqKey="Muller B">B. Müller</name></author><author><name sortKey="Grohmann, D" uniqKey="Grohmann D">D. Grohmann</name></author><author><name sortKey="Westhoff, P" uniqKey="Westhoff P">P. Westhoff</name></author><author><name sortKey="Eichacker, L A" uniqKey="Eichacker L">L. A. Eichacker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yao, Y A" uniqKey="Yao Y">Y. A. Yao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kr L, M" uniqKey="Kr L M">M. Król</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, Y H" uniqKey="Xu Y">Y. H. Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scheibe, R" uniqKey="Scheibe R">R. Scheibe</name></author><author><name sortKey="Dietz, K J" uniqKey="Dietz K">K. J. Dietz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dietz, K J" uniqKey="Dietz K">K. J. Dietz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, K H" uniqKey="Kim K">K. H. Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ambruso, D R" uniqKey="Ambruso D">D. R. Ambruso</name></author><author><name sortKey="Ellison, M A" uniqKey="Ellison M">M. A. Ellison</name></author><author><name sortKey="Thurman, G W" uniqKey="Thurman G">G. W. Thurman</name></author><author><name sortKey="Leto, T L" uniqKey="Leto T">T. L. Leto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dietz, K J" uniqKey="Dietz K">K. J. Dietz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dietz, K J" uniqKey="Dietz K">K. J. Dietz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rhee, S G" uniqKey="Rhee S">S. G. Rhee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ishiguro, S" uniqKey="Ishiguro S">S. Ishiguro</name></author><author><name sortKey="Kawai Oda, A" uniqKey="Kawai Oda A">A. Kawai-Oda</name></author><author><name sortKey="Ueda, K" uniqKey="Ueda K">K. Ueda</name></author><author><name sortKey="Nishida, I" uniqKey="Nishida I">I. Nishida</name></author><author><name sortKey="Okada, K" uniqKey="Okada K">K. Okada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narvaez Vasquez, J" uniqKey="Narvaez Vasquez J">J. Narvaez Vasquez</name></author><author><name sortKey="Florin Christensen, J" uniqKey="Florin Christensen J">J. Florin Christensen</name></author><author><name sortKey="Ryan, C A" uniqKey="Ryan C">C. A. Ryan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, Y" uniqKey="Zhao Y">Y. Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dong, W" uniqKey="Dong W">W. Dong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thomson, M C" uniqKey="Thomson M">M. C. Thomson</name></author><author><name sortKey="Macfarlane, J L" uniqKey="Macfarlane J">J. L. Macfarlane</name></author><author><name sortKey="Beagley, C T" uniqKey="Beagley C">C. T. Beagley</name></author><author><name sortKey="Wolstenholme, D R" uniqKey="Wolstenholme D">D. R. Wolstenholme</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zoschke, R" uniqKey="Zoschke R">R. Zoschke</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zorb, C" uniqKey="Zorb C">C. Zörb</name></author><author><name sortKey="Schmitt, S" uniqKey="Schmitt S">S. Schmitt</name></author><author><name sortKey="Muhling, K H" uniqKey="Muhling K">K. H. Mühling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kirst, H" uniqKey="Kirst H">H. Kirst</name></author><author><name sortKey="Melis, A" uniqKey="Melis A">A. Melis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hutin, C" uniqKey="Hutin C">C. Hutin</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27562633</article-id><article-id pub-id-type="pmc">4999883</article-id><article-id pub-id-type="pii">srep32384</article-id><article-id pub-id-type="doi">10.1038/srep32384</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Wenjing</given-names></name><xref ref-type="aff" rid="a1">1</xref><xref ref-type="author-notes" rid="n1">*</xref></contrib><contrib contrib-type="author"><name><surname>Lv</surname><given-names>Hongjun</given-names></name><xref ref-type="aff" rid="a1">1</xref><xref ref-type="author-notes" rid="n1">*</xref></contrib><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Mingming</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Yongchao</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Qi</surname><given-names>Yueying</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Peng</surname><given-names>Zhenying</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Xia</surname><given-names>Guangmin</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Mengcheng</given-names></name><xref ref-type="corresp" rid="c1">a</xref><xref ref-type="aff" rid="a1">1</xref></contrib><aff id="a1"><label>1</label><institution>The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University</institution>, 27 Shandanan Road, Jinan, Shandong 250100,<country>China</country></aff><aff id="a2"><label>2</label><institution>Bio-Tech Research Center, Shandong Academy of Agricultural Science, Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crop</institution>, Jinan, 250100,<country>China</country></aff></contrib-group><author-notes><corresp id="c1"><label>a</label><email>wangmc@sdu.edu.cn</email></corresp><fn id="n1"><label>*</label><p>These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="epub"><day>26</day><month>08</month><year>2016</year></pub-date><pub-date pub-type="collection"><year>2016</year></pub-date><volume>6</volume><elocation-id>32384</elocation-id><history><date date-type="received"><day>20</day><month>05</month><year>2016</year></date><date date-type="accepted"><day>08</day><month>08</month><year>2016</year></date></history><permissions><copyright-statement>Copyright © 2016, The Author(s)</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>The Author(s)</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><pmc-comment>author-paid</pmc-comment>
<license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract><p>We previously bred a salt tolerant wheat cv. SR3 with bread wheat cv. JN177 as the parent via asymmetric somatic hybridization, and found that the tolerance is partially attributed to the superior photosynthesis capacity. Here, we compared the proteomes of two cultivars to unravel the basis of superior photosynthesis capacity. In the maps of two dimensional difference gel electrophoresis (2D-DIGE), there were 26 differentially expressed proteins (DEPs), including 18 cultivar-based and 8 stress-responsive ones. 21 of 26 DEPs were identified and classified into four categories, including photosynthesis, photosynthesis system stability, linolenic acid metabolism, and protein synthesis in chloroplast. The chloroplast localization of some DEPs confirmed that the identified DEPs function in the chloroplast. The overexpression of a DEP enhanced salt tolerance in <italic>Arabidopsis thaliana</italic>. In line with these data, it is concluded that the contribution of chloroplast to high salinity tolerance of wheat cv. SR3 appears to include higher photosynthesis efficiency by promoting system protection and ROS clearance, stronger production of phytohormone JA by enhancing metabolism activity, and modulating the <italic>in chloroplast</italic> synthesis of proteins.</p></abstract></article-meta></front><floats-group><fig id="f1"><label>Figure 1</label><caption><title>The chloroplast-associated physiological indices in leaves of wheat seedlings.</title><p>(<bold>A</bold>) The content of chlorophyll a. (<bold>B</bold>) The content of chlorophyll b. (<bold>C</bold>) Photosynthetic rate. (<bold>D</bold>) The maximal quantum efficiency. (<bold>E</bold>) The activity of ribulose bisphosphate carboxylase/oxygenase. (<bold>F</bold>) The activity of phosphoribulokinase. (<bold>G</bold>) The JA content. (<bold>H</bold>) The ABA content. Data presented as mean ± standard deviation. Columns marked without different letters indicate no difference in means using the one-way ANOVA LSD analysis (<italic>P</italic> < 0.05).</p></caption><graphic xlink:href="srep32384-f1"></graphic></fig><fig id="f2"><label>Figure 2</label><caption><title>The activities of organelle marker enzymes in leaf extract and isolated chloroplast of SR3.</title><p>Extract: the total proteins extracted from leaves; Chloroplast: the proteins extracted from isolated chloroplast. (<bold>A</bold>) The activity of catalase localizing in peroxisome. (<bold>B</bold>) The activity of alkaline phosphatase localizing in cell membrane. (<bold>C</bold>) The activity of acid phosphatase localizing in lysosome. (<bold>D</bold>) The activity of alcohol dehydrogenase localizing in cytosol. Data presented as mean ± standard deviation. *means the difference between extract and chloroplast was significant using the <italic>t</italic>-test (<italic>P</italic> < 0.05).</p></caption><graphic xlink:href="srep32384-f2"></graphic></fig><fig id="f3"><label>Figure 3</label><caption><title>The differential expressed proteins identified by two-dimensional electrophoresis.</title><p>(<bold>A</bold>) The reference two-dimensional electrophoresis map. The spots labeled with numbers have differential abundance among samples. (<bold>B</bold>) The number of cultivar-based differential expressed proteins that are not responsive to salt between SR3 and JN177. (<bold>C</bold>) The number of stress-responsive differential expressed proteins whose abundance was altered after exposure to NaCl treatment. (<bold>D</bold>) The number of stress-responsive differential expressed proteins that have different abundance between SR3 and JN177. High and low: the spots with high and low abundance in SR3 in comparison to JN177. Up and down: spots had high and low abundance after treated with NaCl.</p></caption><graphic xlink:href="srep32384-f3"></graphic></fig><fig id="f4"><label>Figure 4</label><caption><title>The spots of cultivar-based differential expressed proteins.</title><p>The spots indicated by arrows are differential expressed proteins. Numbers at the left side of spot panels are the spots labeled in <xref ref-type="fig" rid="f3">Fig. 3A</xref> and <xref ref-type="supplementary-material" rid="S1">Supplementary Figure 1</xref>. Ratios below the spot panels are the relative gray values of spots in SR3 in comparison with JN177. <italic>P</italic> values show the significant difference in spot abundance using the <italic>t-</italic>test.</p></caption><graphic xlink:href="srep32384-f4"></graphic></fig><fig id="f5"><label>Figure 5</label><caption><title>The spots of stress-responsive differential expressed proteins.</title><p>The spots indicated by arrows are differential expressed proteins. Numbers at the left side of spot panels are the spots labeled in <xref ref-type="fig" rid="f3">Fig. 3A</xref> and <xref ref-type="supplementary-material" rid="S1">Supplementary Figure S1</xref>. Ratios below the spot panels are the relative grey values of spots with the grey values of JN177 under the control condition as the reference. The spots between two samples marked without different letters indicate no difference in means using the one-way ANOVA LSD analysis (<italic>P</italic> < 0.05).</p></caption><graphic xlink:href="srep32384-f5"></graphic></fig><fig id="f6"><label>Figure 6</label><caption><title>The transcriptional profiles of eight randomly selected differential expressed proteins.</title><p>Relative expression level is defined as the ratio of the transcriptional level to the transcriptional level of JN177 under the control condition. Data presented as mean ± standard deviation. Columns marked without different lowercase letter indicate no difference in means using the one-way ANOVA LSD analysis (<italic>P</italic> < 0.05).</p></caption><graphic xlink:href="srep32384-f6"></graphic></fig><fig id="f7"><label>Figure 7</label><caption><title>The subcellular localization assay of wheat 2-Cys peroxiredoxin BAS1 (spot 17).</title><p>(<bold>A</bold>) Protoplast transformed with an empty vector control expressing GFP alone. (<bold>B</bold>) Protoplast expressing the fused protein of wheat 2-Cys peroxiredoxin BAS1 and GFP. GFP: GFP fluorescence signal; Chlorophyll: chlorophyll autofluorescence signal; Bright light: the field of bright light; Merged: the emergence of GFP fluorescence signal, chlorophyll autofluorescence signal and bright light field.</p></caption><graphic xlink:href="srep32384-f7"></graphic></fig><fig id="f8"><label>Figure 8</label><caption><title>Overexpression of a wheat 2-Cys peroxiredoxin BAS1 (spot 17) enhances salt tolerance in <italic>Arabidopsis thaliana</italic>.</title><p>(<bold>A,B</bold>) Two-day-old <italic>Arabidopsis thaliana</italic> seedlings were subject with none or NaCl treatment for twelve days. (<bold>C,D</bold>) The statistical results of shoot fresh weight (<bold>C</bold>) and root length (<bold>D</bold>) in panels (<bold>A,B</bold>). Data presented as mean ± standard deviation, and in each column set, *indicates significant difference from the control condition using the <italic>t</italic>-test (<italic>P</italic> < 0.05).</p></caption><graphic xlink:href="srep32384-f8"></graphic></fig><table-wrap position="float" id="t1"><label>Table 1</label><caption><title>The identification of differentially expressed proteins.</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="left"></col><col align="left"></col><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50">Spot</th><th align="left" valign="top" charoff="50">Accession No.</th><th align="left" valign="top" charoff="50">Annotation</th><th align="left" valign="top" charoff="50">Species</th><th align="center" valign="top" charoff="50">Tmw</th><th align="center" valign="top" charoff="50">Emw</th><th align="center" valign="top" charoff="50">TpI</th><th align="center" valign="top" charoff="50">EpI</th><th align="center" valign="top" charoff="50">Score</th></tr></thead><tbody valign="top"><tr><td align="left" valign="top" charoff="50">2</td><td align="left" valign="top" charoff="50">PLA20_ARATH</td><td align="left" valign="top" charoff="50">Phospholipase A1-IIdelta</td><td align="left" valign="top" charoff="50"><italic>Arabidopsis thaliana</italic></td><td align="center" valign="top" charoff="50">46.03</td><td align="center" valign="top" charoff="50">14.34</td><td align="center" valign="top" charoff="50">5.11</td><td align="center" valign="top" charoff="50">4.72</td><td align="center" valign="top" charoff="50">64</td></tr><tr><td align="left" valign="top" charoff="50">3</td><td align="left" valign="top" charoff="50">PLA20_ARATH</td><td align="left" valign="top" charoff="50">Phospholipase A1-IIdelta</td><td align="left" valign="top" charoff="50"><italic>Arabidopsis thaliana</italic></td><td align="center" valign="top" charoff="50">46.03</td><td align="center" valign="top" charoff="50">16.01</td><td align="center" valign="top" charoff="50">5.11</td><td align="center" valign="top" charoff="50">6.24</td><td align="center" valign="top" charoff="50">63</td></tr><tr><td align="left" valign="top" charoff="50">4</td><td align="left" valign="top" charoff="50">RK16_NANDO</td><td align="left" valign="top" charoff="50">50S ribosomal protein L16</td><td align="left" valign="top" charoff="50"><italic>Nandina domestica</italic></td><td align="center" valign="top" charoff="50">15.27</td><td align="center" valign="top" charoff="50">15.11</td><td align="center" valign="top" charoff="50">11.39</td><td align="center" valign="top" charoff="50">5.37</td><td align="center" valign="top" charoff="50">53</td></tr><tr><td align="left" valign="top" charoff="50">5</td><td align="left" valign="top" charoff="50">RBS_HORVU</td><td align="left" valign="top" charoff="50">Ribulose bisphosphate carboxylase small chain</td><td align="left" valign="top" charoff="50"><italic>Hordeum vulgare</italic></td><td align="center" valign="top" charoff="50">19.41</td><td align="center" valign="top" charoff="50">14.36</td><td align="center" valign="top" charoff="50">8.98</td><td align="center" valign="top" charoff="50">6.54</td><td align="center" valign="top" charoff="50">97</td></tr><tr><td align="left" valign="top" charoff="50">6</td><td align="left" valign="top" charoff="50">RBS3_WHEAT</td><td align="left" valign="top" charoff="50">Ribulose bisphosphate carboxylase small chain clone 512 (Fragment)</td><td align="left" valign="top" charoff="50"><italic>Triticum aestivum</italic></td><td align="center" valign="top" charoff="50">13.05</td><td align="center" valign="top" charoff="50">15.2</td><td align="center" valign="top" charoff="50">5.84</td><td align="center" valign="top" charoff="50">6.47</td><td align="center" valign="top" charoff="50">119</td></tr><tr><td align="left" valign="top" charoff="50">7</td><td align="left" valign="top" charoff="50">RBS3_WHEAT</td><td align="left" valign="top" charoff="50">Ribulose bisphosphate carboxylase small chain clone 512 (Fragment)</td><td align="left" valign="top" charoff="50"><italic>Triticum aestivum</italic></td><td align="center" valign="top" charoff="50">13.05</td><td align="center" valign="top" charoff="50">15.63</td><td align="center" valign="top" charoff="50">5.84</td><td align="center" valign="top" charoff="50">6.47</td><td align="center" valign="top" charoff="50">76</td></tr><tr><td align="left" valign="top" charoff="50">8</td><td align="left" valign="top" charoff="50">PSBP_WHEAT</td><td align="left" valign="top" charoff="50">Oxygen-evolving enhancer protein 2</td><td align="left" valign="top" charoff="50"><italic>Triticum aestivum</italic></td><td align="center" valign="top" charoff="50">27.25</td><td align="center" valign="top" charoff="50">19.01</td><td align="center" valign="top" charoff="50">8.84</td><td align="center" valign="top" charoff="50">5.35</td><td align="center" valign="top" charoff="50">61</td></tr><tr><td align="left" valign="top" charoff="50">9</td><td align="left" valign="top" charoff="50">gi|475519847</td><td align="left" valign="top" charoff="50">photosystem II stability/assembly factor HCF136</td><td align="left" valign="top" charoff="50"><italic>Aegilops tauschii</italic></td><td align="center" valign="top" charoff="50">41.83</td><td align="center" valign="top" charoff="50">29.58</td><td align="center" valign="top" charoff="50">6.15</td><td align="center" valign="top" charoff="50">5.43</td><td align="center" valign="top" charoff="50">76</td></tr><tr><td align="left" valign="top" charoff="50">10</td><td align="left" valign="top" charoff="50">RK16_COFAR</td><td align="left" valign="top" charoff="50">50S ribosomal protein L16</td><td align="left" valign="top" charoff="50"><italic>Coffea arabica</italic></td><td align="center" valign="top" charoff="50">15.43</td><td align="center" valign="top" charoff="50">25.37</td><td align="center" valign="top" charoff="50">11.89</td><td align="center" valign="top" charoff="50">5.34</td><td align="center" valign="top" charoff="50">65</td></tr><tr><td align="left" valign="top" charoff="50">11</td><td align="left" valign="top" charoff="50">KPPR_WHEAT</td><td align="left" valign="top" charoff="50">Phosphoribulokinase</td><td align="left" valign="top" charoff="50"><italic>Triticum aestivum</italic></td><td align="center" valign="top" charoff="50">45.11</td><td align="center" valign="top" charoff="50">29.46</td><td align="center" valign="top" charoff="50">5.72</td><td align="center" valign="top" charoff="50">5.13</td><td align="center" valign="top" charoff="50">88</td></tr><tr><td align="left" valign="top" charoff="50">12</td><td align="left" valign="top" charoff="50">gi|168057698</td><td align="left" valign="top" charoff="50">predicted protein</td><td align="left" valign="top" charoff="50"><italic>Physcomitrella patens</italic></td><td align="center" valign="top" charoff="50">15.83</td><td align="center" valign="top" charoff="50">17.31</td><td align="center" valign="top" charoff="50">7.85</td><td align="center" valign="top" charoff="50">5.25</td><td align="center" valign="top" charoff="50">72</td></tr><tr><td align="left" valign="top" charoff="50">14</td><td align="left" valign="top" charoff="50">T02253</td><td align="left" valign="top" charoff="50">chlorophyll a/b-binding apoprotein CP24 precursor</td><td align="left" valign="top" charoff="50"><italic>Zea mays</italic></td><td align="center" valign="top" charoff="50">27.01</td><td align="center" valign="top" charoff="50">27.43</td><td align="center" valign="top" charoff="50">9.24</td><td align="center" valign="top" charoff="50">5.14</td><td align="center" valign="top" charoff="50">121</td></tr><tr><td align="left" valign="top" charoff="50">15</td><td align="left" valign="top" charoff="50">KPPR_WHEAT</td><td align="left" valign="top" charoff="50">Phosphoribulokinase</td><td align="left" valign="top" charoff="50"><italic>Triticum aestivum</italic></td><td align="center" valign="top" charoff="50">45.11</td><td align="center" valign="top" charoff="50">29.77</td><td align="center" valign="top" charoff="50">5.72</td><td align="center" valign="top" charoff="50">5.32</td><td align="center" valign="top" charoff="50">70</td></tr><tr><td align="left" valign="top" charoff="50">16</td><td align="left" valign="top" charoff="50">KPPR_WHEAT</td><td align="left" valign="top" charoff="50">Phosphoribulokinase</td><td align="left" valign="top" charoff="50"><italic>Triticum aestivum</italic></td><td align="center" valign="top" charoff="50">45.11</td><td align="center" valign="top" charoff="50">29.77</td><td align="center" valign="top" charoff="50">5.72</td><td align="center" valign="top" charoff="50">5.04</td><td align="center" valign="top" charoff="50">97</td></tr><tr><td align="left" valign="top" charoff="50">17</td><td align="left" valign="top" charoff="50">BAS1_HORVU</td><td align="left" valign="top" charoff="50">2-Cys peroxiredoxin BAS1</td><td align="left" valign="top" charoff="50"><italic>Hordeum vulgare</italic></td><td align="center" valign="top" charoff="50">23.28</td><td align="center" valign="top" charoff="50">16.96</td><td align="center" valign="top" charoff="50">5.48</td><td align="center" valign="top" charoff="50">5.18</td><td align="center" valign="top" charoff="50">63</td></tr><tr><td align="left" valign="top" charoff="50">18</td><td align="left" valign="top" charoff="50">RK16_SACHY</td><td align="left" valign="top" charoff="50">50S ribosomal protein L16</td><td align="left" valign="top" charoff="50"><italic>Saccharum hybrid</italic></td><td align="center" valign="top" charoff="50">15.51</td><td align="center" valign="top" charoff="50">13.22</td><td align="center" valign="top" charoff="50">11.56</td><td align="center" valign="top" charoff="50">4.67</td><td align="center" valign="top" charoff="50">52</td></tr><tr><td align="left" valign="top" charoff="50">19</td><td align="left" valign="top" charoff="50">MATK_CLEFL</td><td align="left" valign="top" charoff="50">Maturase K</td><td align="left" valign="top" charoff="50"><italic>Clematis florida</italic></td><td align="center" valign="top" charoff="50">61.05</td><td align="center" valign="top" charoff="50">13.4</td><td align="center" valign="top" charoff="50">9.55</td><td align="center" valign="top" charoff="50">4.46</td><td align="center" valign="top" charoff="50">61</td></tr><tr><td align="left" valign="top" charoff="50">20</td><td align="left" valign="top" charoff="50">C82C2_ARATH</td><td align="left" valign="top" charoff="50">Cytochrome P450</td><td align="left" valign="top" charoff="50"><italic>Arabidopsis thaliana</italic></td><td align="center" valign="top" charoff="50">58.96</td><td align="center" valign="top" charoff="50">15.23</td><td align="center" valign="top" charoff="50">8.69</td><td align="center" valign="top" charoff="50">4.97</td><td align="center" valign="top" charoff="50">63</td></tr><tr><td align="left" valign="top" charoff="50">23</td><td align="left" valign="top" charoff="50">BAS1_HORVU</td><td align="left" valign="top" charoff="50">2-Cys peroxiredoxin BAS1</td><td align="left" valign="top" charoff="50"><italic>Hordeum vulgare</italic></td><td align="center" valign="top" charoff="50">23.28</td><td align="center" valign="top" charoff="50">16.89</td><td align="center" valign="top" charoff="50">5.48</td><td align="center" valign="top" charoff="50">5.02</td><td align="center" valign="top" charoff="50">60</td></tr><tr><td align="left" valign="top" charoff="50">24</td><td align="left" valign="top" charoff="50">RK16_CALFG</td><td align="left" valign="top" charoff="50">50S ribosomal protein L16</td><td align="left" valign="top" charoff="50"><italic>Calycanthus floridus</italic></td><td align="center" valign="top" charoff="50">15.40</td><td align="center" valign="top" charoff="50">13.86</td><td align="center" valign="top" charoff="50">11.56</td><td align="center" valign="top" charoff="50">5.13</td><td align="center" valign="top" charoff="50">41</td></tr><tr><td align="left" valign="top" charoff="50">25</td><td align="left" valign="top" charoff="50">SR541_SOLLC</td><td align="left" valign="top" charoff="50">Signal recognition particle 54 kDa protein 1</td><td align="left" valign="top" charoff="50"><italic>Solanum lycopersicum</italic></td><td align="center" valign="top" charoff="50">54.79</td><td align="center" valign="top" charoff="50">14.79</td><td align="center" valign="top" charoff="50">9.19</td><td align="center" valign="top" charoff="50">4.82</td><td align="center" valign="top" charoff="50">51</td></tr><tr><td align="left" valign="top" charoff="50">26</td><td align="left" valign="top" charoff="50">RR3_CITSI</td><td align="left" valign="top" charoff="50">30S ribosomal protein S3</td><td align="left" valign="top" charoff="50"><italic>Citrus sinensis</italic></td><td align="center" valign="top" charoff="50">25.27</td><td align="center" valign="top" charoff="50">14.33</td><td align="center" valign="top" charoff="50">9.95</td><td align="center" valign="top" charoff="50">4.81</td><td align="center" valign="top" charoff="50">57</td></tr></tbody></table><table-wrap-foot><fn id="t1-fn1"><p>Spot: The numbers of the proteins labeled in 2-DE reference gel of <xref ref-type="fig" rid="f3">Fig. 3</xref>. Accession No: The NCBInr, MSDB or SwissProt reference numbers of proteins. Tmw: The molecular mass of the predicted protein. Emw: The molecular mass of the protein as estimated by its gel migration. TpI: The isoelectric point of the predicted protein: EpI: the isoelectric point of the protein as estimated by its gel migration. Score: The score of credible identification in Mascot using MS data.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Wenjing" sort="Xu, Wenjing" uniqKey="Xu W" first="Wenjing" last="Xu">Wenjing Xu</name></noRegion><name sortKey="Li, Yongchao" sort="Li, Yongchao" uniqKey="Li Y" first="Yongchao" last="Li">Yongchao Li</name><name sortKey="Lv, Hongjun" sort="Lv, Hongjun" uniqKey="Lv H" first="Hongjun" last="Lv">Hongjun Lv</name><name sortKey="Peng, Zhenying" sort="Peng, Zhenying" uniqKey="Peng Z" first="Zhenying" last="Peng">Zhenying Peng</name><name sortKey="Qi, Yueying" sort="Qi, Yueying" uniqKey="Qi Y" first="Yueying" last="Qi">Yueying Qi</name><name sortKey="Wang, Mengcheng" sort="Wang, Mengcheng" uniqKey="Wang M" first="Mengcheng" last="Wang">Mengcheng Wang</name><name sortKey="Xia, Guangmin" sort="Xia, Guangmin" uniqKey="Xia G" first="Guangmin" last="Xia">Guangmin Xia</name><name sortKey="Zhao, Mingming" sort="Zhao, Mingming" uniqKey="Zhao M" first="Mingming" last="Zhao">Mingming Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000053 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000053 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:4999883
|texte= Proteomic comparison reveals the contribution of chloroplast to salt tolerance of a wheat introgression line
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:27562633" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |