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Citrus psorosis, ringspot, cristacortis and concave gum pathogens are maintained in callus culture

Identifieur interne : 001285 ( PascalFrancis/Curation ); précédent : 001284; suivant : 001286

Citrus psorosis, ringspot, cristacortis and concave gum pathogens are maintained in callus culture

Auteurs : J. Navas-Castillo [Espagne] ; P. Moreno ; N. Duran-Vila

Source :

RBID : Pascal:95-0208603

Descripteurs français

English descriptors

Abstract

Callus cultures were established from citrus explants infected with several virus-like pathogens of the psorosis group (psorosis A, psorosis B, ringspot, cristacortis, or concave gum), and successively subcultured for up to 16 months. «Pineapple» sweet orange or «Duncan» grapefruit seedlings graft-inoculated with callus pieces, and incubated in a temperature-controlled greenhouse, developed symptoms characteristic of these diseases, whereas similar indicator plants inoculated with callus developed from healthy explants remained symptomless. Calli infected with cristacortis or concave gum pathogens induced in young leaves of indicator plants chlorotic flecking and, occasionally, an oak leaf pattern. Those infected with psorosis (A or B) or ringspot induced a shock reaction in the first flush, and chlorotic flecks in young leaves developed in successive flushes. Calli from psorosis B-infected plants caused in addition blisters in mature shoots and chlorotic blotches in old leaves with gummy pustules in the underside. These results indicate that the agents causing diseases of the psorosis group can be maintained in callus culture. Nevertheless, trials to purify and detect a specific 48K-protein associated with psorosis and ringspot isolates from infected calli were unsuccessful
pA  
A01 01  1    @0 0167-6857
A02 01      @0 PTCEDJ
A03   1    @0 Plant cell tissue organ cult.
A05       @2 40
A06       @2 2
A08 01  1  ENG  @1 Citrus psorosis, ringspot, cristacortis and concave gum pathogens are maintained in callus culture
A11 01  1    @1 NAVAS-CASTILLO (J.)
A11 02  1    @1 MORENO (P.)
A11 03  1    @1 DURAN-VILA (N.)
A14 01      @1 Inst. Valenciano investigaciones agrarias @2 46113 Moncada Valencia @3 ESP
A20       @1 133-137
A21       @1 1995
A23 01      @0 ENG
A43 01      @1 INIST @2 19374 @5 354000059735900050
A44       @0 0000
A45       @0 27 ref.
A47 01  1    @0 95-0208603
A60       @1 P
A61       @0 A
A64 01  1    @0 Plant cell, tissue and organ culture
A66 01      @0 NLD
C01 01    ENG  @0 Callus cultures were established from citrus explants infected with several virus-like pathogens of the psorosis group (psorosis A, psorosis B, ringspot, cristacortis, or concave gum), and successively subcultured for up to 16 months. «Pineapple» sweet orange or «Duncan» grapefruit seedlings graft-inoculated with callus pieces, and incubated in a temperature-controlled greenhouse, developed symptoms characteristic of these diseases, whereas similar indicator plants inoculated with callus developed from healthy explants remained symptomless. Calli infected with cristacortis or concave gum pathogens induced in young leaves of indicator plants chlorotic flecking and, occasionally, an oak leaf pattern. Those infected with psorosis (A or B) or ringspot induced a shock reaction in the first flush, and chlorotic flecks in young leaves developed in successive flushes. Calli from psorosis B-infected plants caused in addition blisters in mature shoots and chlorotic blotches in old leaves with gummy pustules in the underside. These results indicate that the agents causing diseases of the psorosis group can be maintained in callus culture. Nevertheless, trials to purify and detect a specific 48K-protein associated with psorosis and ringspot isolates from infected calli were unsuccessful
C02 01  X    @0 002A34E02
C03 01  X  FRE  @0 Culture tissu @5 01
C03 01  X  ENG  @0 Tissue culture @5 01
C03 01  X  SPA  @0 Cultivo tejido @5 01
C03 02  X  FRE  @0 Cal @5 02
C03 02  X  ENG  @0 Callus @5 02
C03 02  X  SPA  @0 Callo @5 02
C03 03  X  FRE  @0 Pouvoir infectant @5 09
C03 03  X  ENG  @0 Infectivity @5 09
C03 03  X  SPA  @0 Poder infectante @5 09
C03 04  X  FRE  @0 Virose @5 10
C03 04  X  ENG  @0 Viral disease @5 10
C03 04  X  SPA  @0 Virosis @5 10
C03 05  X  FRE  @0 Symptomatologie @5 11
C03 05  X  ENG  @0 Symptomatology @5 11
C03 05  X  SPA  @0 Sintomatología @5 11
C03 06  X  FRE  @0 Citrus sinensis @2 NS @5 14
C03 06  X  ENG  @0 Citrus sinensis @2 NS @5 14
C03 06  X  SPA  @0 Citrus sinensis @2 NS @5 14
C03 07  X  FRE  @0 Poncirus trifoliata @2 NS @5 15
C03 07  X  ENG  @0 Poncirus trifoliata @2 NS @5 15
C03 07  X  SPA  @0 Poncirus trifoliata @2 NS @5 15
C03 08  X  FRE  @0 Végétal @5 19
C03 08  X  ENG  @0 Vegetals @5 19
C03 08  X  SPA  @0 Vegetal @5 19
C03 09  X  FRE  @0 Agrume @5 45
C03 09  X  ENG  @0 Citrus fruit @5 45
C03 09  X  SPA  @0 Agrios @5 45
C03 10  X  FRE  @0 Phytopathogène @5 67
C03 10  X  ENG  @0 Plant pathogen @5 67
C03 10  X  SPA  @0 Fitopatógeno @5 67
C03 11  X  FRE  @0 Virus tache annulaire agrume @2 NW @4 INC @5 88
C03 12  X  FRE  @0 Virus psorose agrume @2 NW @4 INC @5 89
C03 13  X  FRE  @0 Virus cristacortis agrume @2 NW @4 INC @5 90
C07 01  X  FRE  @0 Infection
C07 01  X  ENG  @0 Infection
C07 01  X  SPA  @0 Infección
C07 02  X  FRE  @0 Rutaceae @2 NS
C07 02  X  ENG  @0 Rutaceae @2 NS
C07 02  X  SPA  @0 Rutaceae @2 NS
C07 03  X  FRE  @0 Dicotyledones @2 NS
C07 03  X  ENG  @0 Dicotyledones @2 NS
C07 03  X  SPA  @0 Dicotyledones @2 NS
C07 04  X  FRE  @0 Angiospermae @2 NS
C07 04  X  ENG  @0 Angiospermae @2 NS
C07 04  X  SPA  @0 Angiospermae @2 NS
C07 05  X  FRE  @0 Spermatophyta @2 NS
C07 05  X  ENG  @0 Spermatophyta @2 NS
C07 05  X  SPA  @0 Spermatophyta @2 NS
C07 06  X  FRE  @0 Viroïde @2 NW @5 68
C07 06  X  ENG  @0 Viroid @2 NW @5 68
C07 06  X  SPA  @0 Viroid @2 NW @5 68
C07 07  X  FRE  @0 Virus @2 NW
C07 07  X  ENG  @0 Virus @2 NW
C07 07  X  SPA  @0 Virus @2 NW
N21       @1 123

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Pascal:95-0208603

Le document en format XML

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<div type="abstract" xml:lang="en">Callus cultures were established from citrus explants infected with several virus-like pathogens of the psorosis group (psorosis A, psorosis B, ringspot, cristacortis, or concave gum), and successively subcultured for up to 16 months. «Pineapple» sweet orange or «Duncan» grapefruit seedlings graft-inoculated with callus pieces, and incubated in a temperature-controlled greenhouse, developed symptoms characteristic of these diseases, whereas similar indicator plants inoculated with callus developed from healthy explants remained symptomless. Calli infected with cristacortis or concave gum pathogens induced in young leaves of indicator plants chlorotic flecking and, occasionally, an oak leaf pattern. Those infected with psorosis (A or B) or ringspot induced a shock reaction in the first flush, and chlorotic flecks in young leaves developed in successive flushes. Calli from psorosis B-infected plants caused in addition blisters in mature shoots and chlorotic blotches in old leaves with gummy pustules in the underside. These results indicate that the agents causing diseases of the psorosis group can be maintained in callus culture. Nevertheless, trials to purify and detect a specific 48K-protein associated with psorosis and ringspot isolates from infected calli were unsuccessful</div>
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<s0>Callus cultures were established from citrus explants infected with several virus-like pathogens of the psorosis group (psorosis A, psorosis B, ringspot, cristacortis, or concave gum), and successively subcultured for up to 16 months. «Pineapple» sweet orange or «Duncan» grapefruit seedlings graft-inoculated with callus pieces, and incubated in a temperature-controlled greenhouse, developed symptoms characteristic of these diseases, whereas similar indicator plants inoculated with callus developed from healthy explants remained symptomless. Calli infected with cristacortis or concave gum pathogens induced in young leaves of indicator plants chlorotic flecking and, occasionally, an oak leaf pattern. Those infected with psorosis (A or B) or ringspot induced a shock reaction in the first flush, and chlorotic flecks in young leaves developed in successive flushes. Calli from psorosis B-infected plants caused in addition blisters in mature shoots and chlorotic blotches in old leaves with gummy pustules in the underside. These results indicate that the agents causing diseases of the psorosis group can be maintained in callus culture. Nevertheless, trials to purify and detect a specific 48K-protein associated with psorosis and ringspot isolates from infected calli were unsuccessful</s0>
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<s5>02</s5>
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<s0>Pouvoir infectant</s0>
<s5>09</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Infectivity</s0>
<s5>09</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Poder infectante</s0>
<s5>09</s5>
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<fC03 i1="04" i2="X" l="FRE">
<s0>Virose</s0>
<s5>10</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Viral disease</s0>
<s5>10</s5>
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<fC03 i1="04" i2="X" l="SPA">
<s0>Virosis</s0>
<s5>10</s5>
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<fC03 i1="05" i2="X" l="FRE">
<s0>Symptomatologie</s0>
<s5>11</s5>
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<s5>11</s5>
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<s5>11</s5>
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<s5>15</s5>
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<s5>15</s5>
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<s5>19</s5>
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<fC03 i1="08" i2="X" l="ENG">
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<s5>19</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
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<s5>45</s5>
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<s5>45</s5>
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<s0>Agrios</s0>
<s5>45</s5>
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<fC03 i1="10" i2="X" l="FRE">
<s0>Phytopathogène</s0>
<s5>67</s5>
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<s0>Plant pathogen</s0>
<s5>67</s5>
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<fC03 i1="10" i2="X" l="SPA">
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<s5>67</s5>
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<s0>Virus tache annulaire agrume</s0>
<s2>NW</s2>
<s4>INC</s4>
<s5>88</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE">
<s0>Virus psorose agrume</s0>
<s2>NW</s2>
<s4>INC</s4>
<s5>89</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE">
<s0>Virus cristacortis agrume</s0>
<s2>NW</s2>
<s4>INC</s4>
<s5>90</s5>
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<fC07 i1="01" i2="X" l="FRE">
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<fC07 i1="01" i2="X" l="ENG">
<s0>Infection</s0>
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<fC07 i1="01" i2="X" l="SPA">
<s0>Infección</s0>
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<fC07 i1="02" i2="X" l="FRE">
<s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="02" i2="X" l="ENG">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="FRE">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Viroïde</s0>
<s2>NW</s2>
<s5>68</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Viroid</s0>
<s2>NW</s2>
<s5>68</s5>
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<fC07 i1="06" i2="X" l="SPA">
<s0>Viroid</s0>
<s2>NW</s2>
<s5>68</s5>
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   |texte=   Citrus psorosis, ringspot, cristacortis and concave gum pathogens are maintained in callus culture
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