Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments
Identifieur interne : 000D16 ( PascalFrancis/Corpus ); précédent : 000D15; suivant : 000D17Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments
Auteurs : M. L. Marin ; Y. Gogorcena ; J. Ortiz ; N. Duran-VilaSource :
- Plant cell, tissue and organ culture [ 0167-6857 ] ; 1993.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min-1 down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
pA |
|
---|
Format Inist (serveur)
NO : | PASCAL 93-0651874 INIST |
---|---|
ET : | Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments |
AU : | MARIN (M. L.); GOGORCENA (Y.); ORTIZ (J.); DURAN-VILA (N.) |
AF : | Inst. Valenciano investigaciones agrarias/46113 Moncada/Espagne |
DT : | Publication en série; Niveau analytique |
SO : | Plant cell, tissue and organ culture; ISSN 0167-6857; Coden PTCEDJ; Pays-Bas; Da. 1993; Vol. 34; No. 1; Pp. 27-33; Bibl. 21 ref. |
LA : | Anglais |
EA : | Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min-1 down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years |
CC : | 002A31C07C2; 215 |
FD : | Cryoconservation; Embryon somatique; Régénération; Gel dégel; Germoplasme; Culture tissu; Végétal; Citrus sinensis; Agrume |
FG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta |
ED : | Cryopreservation; Somatic embryo; Regeneration; Freeze thaw cycle; Germplasm; Tissue culture; Vegetals; Citrus sinensis; Citrus fruit |
EG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta |
SD : | Crioconservación; Embrión somático; Regeneración; Hielo deshielo; Germoplasma; Cultivo tejido; Vegetal; Citrus sinensis; Agrios |
LO : | INIST-19374.354000035064000040 |
ID : | 93-0651874 |
Links to Exploration step
Pascal:93-0651874Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</title>
<author><name sortKey="Marin, M L" sort="Marin, M L" uniqKey="Marin M" first="M. L." last="Marin">M. L. Marin</name>
<affiliation><inist:fA14 i1="01"><s1>Inst. Valenciano investigaciones agrarias</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Gogorcena, Y" sort="Gogorcena, Y" uniqKey="Gogorcena Y" first="Y." last="Gogorcena">Y. Gogorcena</name>
</author>
<author><name sortKey="Ortiz, J" sort="Ortiz, J" uniqKey="Ortiz J" first="J." last="Ortiz">J. Ortiz</name>
</author>
<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">93-0651874</idno>
<date when="1993">1993</date>
<idno type="stanalyst">PASCAL 93-0651874 INIST</idno>
<idno type="RBID">Pascal:93-0651874</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000D16</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</title>
<author><name sortKey="Marin, M L" sort="Marin, M L" uniqKey="Marin M" first="M. L." last="Marin">M. L. Marin</name>
<affiliation><inist:fA14 i1="01"><s1>Inst. Valenciano investigaciones agrarias</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Gogorcena, Y" sort="Gogorcena, Y" uniqKey="Gogorcena Y" first="Y." last="Gogorcena">Y. Gogorcena</name>
</author>
<author><name sortKey="Ortiz, J" sort="Ortiz, J" uniqKey="Ortiz J" first="J." last="Ortiz">J. Ortiz</name>
</author>
<author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
</author>
</analytic>
<series><title level="j" type="main">Plant cell, tissue and organ culture</title>
<title level="j" type="abbreviated">Plant cell tissue organ cult.</title>
<idno type="ISSN">0167-6857</idno>
<imprint><date when="1993">1993</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant cell, tissue and organ culture</title>
<title level="j" type="abbreviated">Plant cell tissue organ cult.</title>
<idno type="ISSN">0167-6857</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Cryopreservation</term>
<term>Freeze thaw cycle</term>
<term>Germplasm</term>
<term>Regeneration</term>
<term>Somatic embryo</term>
<term>Tissue culture</term>
<term>Vegetals</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Cryoconservation</term>
<term>Embryon somatique</term>
<term>Régénération</term>
<term>Gel dégel</term>
<term>Germoplasme</term>
<term>Culture tissu</term>
<term>Végétal</term>
<term>Citrus sinensis</term>
<term>Agrume</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min<sup>-1</sup>
down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0167-6857</s0>
</fA01>
<fA02 i1="01"><s0>PTCEDJ</s0>
</fA02>
<fA03 i2="1"><s0>Plant cell tissue organ cult.</s0>
</fA03>
<fA05><s2>34</s2>
</fA05>
<fA06><s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>MARIN (M. L.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>GOGORCENA (Y.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>ORTIZ (J.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>DURAN-VILA (N.)</s1>
</fA11>
<fA14 i1="01"><s1>Inst. Valenciano investigaciones agrarias</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
</fA14>
<fA20><s1>27-33</s1>
</fA20>
<fA21><s1>1993</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>19374</s2>
<s5>354000035064000040</s5>
</fA43>
<fA44><s0>0000</s0>
</fA44>
<fA45><s0>21 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>93-0651874</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i2="1"><s0>Plant cell, tissue and organ culture</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min<sup>-1</sup>
down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31C07C2</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Cryoconservation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Cryopreservation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Crioconservación</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Embryon somatique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Somatic embryo</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Embrión somático</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Régénération</s0>
<s5>09</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Regeneration</s0>
<s5>09</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Regeneración</s0>
<s5>09</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Gel dégel</s0>
<s5>13</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Freeze thaw cycle</s0>
<s5>13</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Hielo deshielo</s0>
<s5>13</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Germoplasme</s0>
<s5>17</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Germplasm</s0>
<s5>17</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Germoplasma</s0>
<s5>17</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Culture tissu</s0>
<s5>21</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Tissue culture</s0>
<s5>21</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Cultivo tejido</s0>
<s5>21</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Végétal</s0>
<s5>22</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Vegetals</s0>
<s5>22</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Vegetal</s0>
<s5>22</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>64</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>64</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>64</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Agrume</s0>
<s5>65</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>65</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Agrios</s0>
<s5>65</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>341</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 93-0651874 INIST</NO>
<ET>Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</ET>
<AU>MARIN (M. L.); GOGORCENA (Y.); ORTIZ (J.); DURAN-VILA (N.)</AU>
<AF>Inst. Valenciano investigaciones agrarias/46113 Moncada/Espagne</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant cell, tissue and organ culture; ISSN 0167-6857; Coden PTCEDJ; Pays-Bas; Da. 1993; Vol. 34; No. 1; Pp. 27-33; Bibl. 21 ref.</SO>
<LA>Anglais</LA>
<EA>Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min<sup>-1</sup>
down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years</EA>
<CC>002A31C07C2; 215</CC>
<FD>Cryoconservation; Embryon somatique; Régénération; Gel dégel; Germoplasme; Culture tissu; Végétal; Citrus sinensis; Agrume</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Cryopreservation; Somatic embryo; Regeneration; Freeze thaw cycle; Germplasm; Tissue culture; Vegetals; Citrus sinensis; Citrus fruit</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Crioconservación; Embrión somático; Regeneración; Hielo deshielo; Germoplasma; Cultivo tejido; Vegetal; Citrus sinensis; Agrios</SD>
<LO>INIST-19374.354000035064000040</LO>
<ID>93-0651874</ID>
</server>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PascalFrancis/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D16 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000D16 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= PascalFrancis |étape= Corpus |type= RBID |clé= Pascal:93-0651874 |texte= Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments }}
![]() | This area was generated with Dilib version V0.6.25. | ![]() |