Serveur d'exploration sur l'oranger

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments

Identifieur interne : 000D16 ( PascalFrancis/Corpus ); précédent : 000D15; suivant : 000D17

Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments

Auteurs : M. L. Marin ; Y. Gogorcena ; J. Ortiz ; N. Duran-Vila

Source :

RBID : Pascal:93-0651874

Descripteurs français

English descriptors

Abstract

Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min-1 down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0167-6857
A02 01      @0 PTCEDJ
A03   1    @0 Plant cell tissue organ cult.
A05       @2 34
A06       @2 1
A08 01  1  ENG  @1 Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments
A11 01  1    @1 MARIN (M. L.)
A11 02  1    @1 GOGORCENA (Y.)
A11 03  1    @1 ORTIZ (J.)
A11 04  1    @1 DURAN-VILA (N.)
A14 01      @1 Inst. Valenciano investigaciones agrarias @2 46113 Moncada @3 ESP
A20       @1 27-33
A21       @1 1993
A23 01      @0 ENG
A43 01      @1 INIST @2 19374 @5 354000035064000040
A44       @0 0000
A45       @0 21 ref.
A47 01  1    @0 93-0651874
A60       @1 P
A61       @0 A
A64   1    @0 Plant cell, tissue and organ culture
A66 01      @0 NLD
C01 01    ENG  @0 Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min-1 down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years
C02 01  X    @0 002A31C07C2
C02 02  X    @0 215
C03 01  X  FRE  @0 Cryoconservation @5 01
C03 01  X  ENG  @0 Cryopreservation @5 01
C03 01  X  SPA  @0 Crioconservación @5 01
C03 02  X  FRE  @0 Embryon somatique @5 05
C03 02  X  ENG  @0 Somatic embryo @5 05
C03 02  X  SPA  @0 Embrión somático @5 05
C03 03  X  FRE  @0 Régénération @5 09
C03 03  X  ENG  @0 Regeneration @5 09
C03 03  X  SPA  @0 Regeneración @5 09
C03 04  X  FRE  @0 Gel dégel @5 13
C03 04  X  ENG  @0 Freeze thaw cycle @5 13
C03 04  X  SPA  @0 Hielo deshielo @5 13
C03 05  X  FRE  @0 Germoplasme @5 17
C03 05  X  ENG  @0 Germplasm @5 17
C03 05  X  SPA  @0 Germoplasma @5 17
C03 06  X  FRE  @0 Culture tissu @5 21
C03 06  X  ENG  @0 Tissue culture @5 21
C03 06  X  SPA  @0 Cultivo tejido @5 21
C03 07  X  FRE  @0 Végétal @5 22
C03 07  X  ENG  @0 Vegetals @5 22
C03 07  X  SPA  @0 Vegetal @5 22
C03 08  X  FRE  @0 Citrus sinensis @2 NS @5 64
C03 08  X  ENG  @0 Citrus sinensis @2 NS @5 64
C03 08  X  SPA  @0 Citrus sinensis @2 NS @5 64
C03 09  X  FRE  @0 Agrume @5 65
C03 09  X  ENG  @0 Citrus fruit @5 65
C03 09  X  SPA  @0 Agrios @5 65
C07 01  X  FRE  @0 Rutaceae @2 NS
C07 01  X  ENG  @0 Rutaceae @2 NS
C07 01  X  SPA  @0 Rutaceae @2 NS
C07 02  X  FRE  @0 Dicotyledones @2 NS
C07 02  X  ENG  @0 Dicotyledones @2 NS
C07 02  X  SPA  @0 Dicotyledones @2 NS
C07 03  X  FRE  @0 Angiospermae @2 NS
C07 03  X  ENG  @0 Angiospermae @2 NS
C07 03  X  SPA  @0 Angiospermae @2 NS
C07 04  X  FRE  @0 Spermatophyta @2 NS
C07 04  X  ENG  @0 Spermatophyta @2 NS
C07 04  X  SPA  @0 Spermatophyta @2 NS
N21       @1 341

Format Inist (serveur)

NO : PASCAL 93-0651874 INIST
ET : Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments
AU : MARIN (M. L.); GOGORCENA (Y.); ORTIZ (J.); DURAN-VILA (N.)
AF : Inst. Valenciano investigaciones agrarias/46113 Moncada/Espagne
DT : Publication en série; Niveau analytique
SO : Plant cell, tissue and organ culture; ISSN 0167-6857; Coden PTCEDJ; Pays-Bas; Da. 1993; Vol. 34; No. 1; Pp. 27-33; Bibl. 21 ref.
LA : Anglais
EA : Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min-1 down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years
CC : 002A31C07C2; 215
FD : Cryoconservation; Embryon somatique; Régénération; Gel dégel; Germoplasme; Culture tissu; Végétal; Citrus sinensis; Agrume
FG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
ED : Cryopreservation; Somatic embryo; Regeneration; Freeze thaw cycle; Germplasm; Tissue culture; Vegetals; Citrus sinensis; Citrus fruit
EG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
SD : Crioconservación; Embrión somático; Regeneración; Hielo deshielo; Germoplasma; Cultivo tejido; Vegetal; Citrus sinensis; Agrios
LO : INIST-19374.354000035064000040
ID : 93-0651874

Links to Exploration step

Pascal:93-0651874

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en" level="a">Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</title>
<author>
<name sortKey="Marin, M L" sort="Marin, M L" uniqKey="Marin M" first="M. L." last="Marin">M. L. Marin</name>
<affiliation>
<inist:fA14 i1="01">
<s1>Inst. Valenciano investigaciones agrarias</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
</inist:fA14>
</affiliation>
</author>
<author>
<name sortKey="Gogorcena, Y" sort="Gogorcena, Y" uniqKey="Gogorcena Y" first="Y." last="Gogorcena">Y. Gogorcena</name>
</author>
<author>
<name sortKey="Ortiz, J" sort="Ortiz, J" uniqKey="Ortiz J" first="J." last="Ortiz">J. Ortiz</name>
</author>
<author>
<name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">INIST</idno>
<idno type="inist">93-0651874</idno>
<date when="1993">1993</date>
<idno type="stanalyst">PASCAL 93-0651874 INIST</idno>
<idno type="RBID">Pascal:93-0651874</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000D16</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en" level="a">Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</title>
<author>
<name sortKey="Marin, M L" sort="Marin, M L" uniqKey="Marin M" first="M. L." last="Marin">M. L. Marin</name>
<affiliation>
<inist:fA14 i1="01">
<s1>Inst. Valenciano investigaciones agrarias</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
</inist:fA14>
</affiliation>
</author>
<author>
<name sortKey="Gogorcena, Y" sort="Gogorcena, Y" uniqKey="Gogorcena Y" first="Y." last="Gogorcena">Y. Gogorcena</name>
</author>
<author>
<name sortKey="Ortiz, J" sort="Ortiz, J" uniqKey="Ortiz J" first="J." last="Ortiz">J. Ortiz</name>
</author>
<author>
<name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name>
</author>
</analytic>
<series>
<title level="j" type="main">Plant cell, tissue and organ culture</title>
<title level="j" type="abbreviated">Plant cell tissue organ cult.</title>
<idno type="ISSN">0167-6857</idno>
<imprint>
<date when="1993">1993</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt>
<title level="j" type="main">Plant cell, tissue and organ culture</title>
<title level="j" type="abbreviated">Plant cell tissue organ cult.</title>
<idno type="ISSN">0167-6857</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Cryopreservation</term>
<term>Freeze thaw cycle</term>
<term>Germplasm</term>
<term>Regeneration</term>
<term>Somatic embryo</term>
<term>Tissue culture</term>
<term>Vegetals</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr">
<term>Cryoconservation</term>
<term>Embryon somatique</term>
<term>Régénération</term>
<term>Gel dégel</term>
<term>Germoplasme</term>
<term>Culture tissu</term>
<term>Végétal</term>
<term>Citrus sinensis</term>
<term>Agrume</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min
<sup>-1</sup>
down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years</div>
</front>
</TEI>
<inist>
<standard h6="B">
<pA>
<fA01 i1="01" i2="1">
<s0>0167-6857</s0>
</fA01>
<fA02 i1="01">
<s0>PTCEDJ</s0>
</fA02>
<fA03 i2="1">
<s0>Plant cell tissue organ cult.</s0>
</fA03>
<fA05>
<s2>34</s2>
</fA05>
<fA06>
<s2>1</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG">
<s1>Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</s1>
</fA08>
<fA11 i1="01" i2="1">
<s1>MARIN (M. L.)</s1>
</fA11>
<fA11 i1="02" i2="1">
<s1>GOGORCENA (Y.)</s1>
</fA11>
<fA11 i1="03" i2="1">
<s1>ORTIZ (J.)</s1>
</fA11>
<fA11 i1="04" i2="1">
<s1>DURAN-VILA (N.)</s1>
</fA11>
<fA14 i1="01">
<s1>Inst. Valenciano investigaciones agrarias</s1>
<s2>46113 Moncada</s2>
<s3>ESP</s3>
</fA14>
<fA20>
<s1>27-33</s1>
</fA20>
<fA21>
<s1>1993</s1>
</fA21>
<fA23 i1="01">
<s0>ENG</s0>
</fA23>
<fA43 i1="01">
<s1>INIST</s1>
<s2>19374</s2>
<s5>354000035064000040</s5>
</fA43>
<fA44>
<s0>0000</s0>
</fA44>
<fA45>
<s0>21 ref.</s0>
</fA45>
<fA47 i1="01" i2="1">
<s0>93-0651874</s0>
</fA47>
<fA60>
<s1>P</s1>
</fA60>
<fA61>
<s0>A</s0>
</fA61>
<fA64 i2="1">
<s0>Plant cell, tissue and organ culture</s0>
</fA64>
<fA66 i1="01">
<s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG">
<s0>Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min
<sup>-1</sup>
down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years</s0>
</fC01>
<fC02 i1="01" i2="X">
<s0>002A31C07C2</s0>
</fC02>
<fC02 i1="02" i2="X">
<s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE">
<s0>Cryoconservation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG">
<s0>Cryopreservation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA">
<s0>Crioconservación</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Embryon somatique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Somatic embryo</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Embrión somático</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Régénération</s0>
<s5>09</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Regeneration</s0>
<s5>09</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Regeneración</s0>
<s5>09</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Gel dégel</s0>
<s5>13</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Freeze thaw cycle</s0>
<s5>13</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Hielo deshielo</s0>
<s5>13</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Germoplasme</s0>
<s5>17</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Germplasm</s0>
<s5>17</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Germoplasma</s0>
<s5>17</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Culture tissu</s0>
<s5>21</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>Tissue culture</s0>
<s5>21</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>Cultivo tejido</s0>
<s5>21</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE">
<s0>Végétal</s0>
<s5>22</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Vegetals</s0>
<s5>22</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
<s0>Vegetal</s0>
<s5>22</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>64</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>64</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>64</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE">
<s0>Agrume</s0>
<s5>65</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG">
<s0>Citrus fruit</s0>
<s5>65</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA">
<s0>Agrios</s0>
<s5>65</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fN21>
<s1>341</s1>
</fN21>
</pA>
</standard>
<server>
<NO>PASCAL 93-0651874 INIST</NO>
<ET>Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments</ET>
<AU>MARIN (M. L.); GOGORCENA (Y.); ORTIZ (J.); DURAN-VILA (N.)</AU>
<AF>Inst. Valenciano investigaciones agrarias/46113 Moncada/Espagne</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant cell, tissue and organ culture; ISSN 0167-6857; Coden PTCEDJ; Pays-Bas; Da. 1993; Vol. 34; No. 1; Pp. 27-33; Bibl. 21 ref.</SO>
<LA>Anglais</LA>
<EA>Freezing/thawing conditions for cryopreservation of somatic embryos of «Washington Navel» sweet orange (Citrus sinensis (L.) Osb.) were evaluated. No survival of fast-cooled embryos occurred regardless of the thawing method. Embryos subjected to slow cooling at an estimated rate of 0.5°C min
<sup>-1</sup>
down to -42°C followed by immersion in liquid nitrogen survived. Survival rate depended on the thawing method. An average survival of 30.5% was achieved when frozen embryos were thawed by immersion in a water bath at 37°C. Surviving embryos developed into whole plantlets and no phenotypic abnormalities have been observed during a growth period of four years</EA>
<CC>002A31C07C2; 215</CC>
<FD>Cryoconservation; Embryon somatique; Régénération; Gel dégel; Germoplasme; Culture tissu; Végétal; Citrus sinensis; Agrume</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Cryopreservation; Somatic embryo; Regeneration; Freeze thaw cycle; Germplasm; Tissue culture; Vegetals; Citrus sinensis; Citrus fruit</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Crioconservación; Embrión somático; Regeneración; Hielo deshielo; Germoplasma; Cultivo tejido; Vegetal; Citrus sinensis; Agrios</SD>
<LO>INIST-19374.354000035064000040</LO>
<ID>93-0651874</ID>
</server>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PascalFrancis/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D16 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000D16 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    PascalFrancis
   |étape=   Corpus
   |type=    RBID
   |clé=     Pascal:93-0651874
   |texte=   Recovery of whole plants of sweet orange from somatic embryos subjected to freezing thawing treatments
}}

Wicri

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024