DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus
Identifieur interne : 000C05 ( PascalFrancis/Corpus ); précédent : 000C04; suivant : 000C06DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus
Auteurs : F. Luro ; F. Laigret ; J.-M. Bove ; P. OllitraultSource :
- HortScience [ 0018-5345 ] ; 1995.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L.) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique for discriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 C. reticulata cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related.
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Format Inist (serveur)
NO : | PASCAL 95-0500201 INIST |
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ET : | DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus |
AU : | LURO (F.); LAIGRET (F.); BOVE (J.-M.); OLLITRAULT (P.) |
AF : | INRA, cent. coopération internationale rech. agronomique développement-fruits légumes horticoles, stn. rech. agronomique Corse/20230 San Nicolao/France (1 aut.); INRA Univ. Bordaux II, lab. biologie cellulaire moléculaire/33883 Villenave d'Ornon/France (2 aut., 3 aut.); cent. coopération internationale rech. agronomique développement-fruits légumes horticoles/34032 Montpellier/France (4 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | HortScience; ISSN 0018-5345; Coden HJHSAR; Etats-Unis; Da. 1995; Vol. 30; No. 5; Pp. 1063-1067; Bibl. 27 ref. |
LA : | Anglais |
EA : | We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L.) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique for discriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 C. reticulata cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related. |
CC : | 002A32D01D4 |
FD : | Etude en laboratoire; Variabilité génétique; Séquence répétée; Méthode fingerprint; Amplification génique; Réaction chaîne polymérase; Cultivar; Citrus volkameriana; Citrus sinensis; Citrus reticulata; DNA; Diversité génétique |
FG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Amélioration génétique; Biologie moléculaire; Méthode analyse; Arbre fruitier; Plante fruitière; Agrume; Acide nucléique; Diversité |
ED : | Laboratory study; Genetic variability; Repeated sequence; Fingerprint method; Gene amplification; Polymerase chain reaction; Cultivar; Citrus volkameriana; Citrus sinensis; Citrus reticulata; DNA; Genetic diversity |
EG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Genetic improvement; Molecular biology; Analysis method; Fruit tree; Fruit crop; Citrus fruit; Nucleic acid; Diversity |
SD : | Estudio en laboratorio; Variabilidad genética; Secuencia repetida; Método fingerprint; Amplificación génica; Reacción cadena polimerasa; Cultivar; Citrus volkameriana; Citrus sinensis; Citrus reticulata; DNA; Diversidád genética |
LO : | INIST-13300.354000053922700380 |
ID : | 95-0500201 |
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Pascal:95-0500201Le document en format XML
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<term>DNA</term>
<term>Fingerprint method</term>
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<term>Laboratory study</term>
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<term>Amplification génique</term>
<term>Réaction chaîne polymérase</term>
<term>Cultivar</term>
<term>Citrus volkameriana</term>
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<front><div type="abstract" xml:lang="en">We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L.) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique for discriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 C. reticulata cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related.</div>
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<s5>02</s5>
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<s5>03</s5>
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<s5>03</s5>
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<s5>03</s5>
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<s5>04</s5>
</fC03>
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<s5>04</s5>
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<fC03 i1="04" i2="X" l="SPA"><s0>Método fingerprint</s0>
<s5>04</s5>
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<s5>05</s5>
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<s5>05</s5>
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<s5>05</s5>
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<s5>06</s5>
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<s5>06</s5>
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<s5>06</s5>
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<s2>NS</s2>
<s5>11</s5>
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<fC03 i1="08" i2="X" l="ENG"><s0>Citrus volkameriana</s0>
<s2>NS</s2>
<s5>11</s5>
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<fC03 i1="08" i2="X" l="SPA"><s0>Citrus volkameriana</s0>
<s2>NS</s2>
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<s2>NS</s2>
<s5>12</s5>
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<fC03 i1="09" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
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<fC03 i1="09" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
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<fC03 i1="10" i2="X" l="FRE"><s0>Citrus reticulata</s0>
<s2>NS</s2>
<s5>13</s5>
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<fC03 i1="10" i2="X" l="ENG"><s0>Citrus reticulata</s0>
<s2>NS</s2>
<s5>13</s5>
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<fC03 i1="10" i2="X" l="SPA"><s0>Citrus reticulata</s0>
<s2>NS</s2>
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<s5>15</s5>
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<fC03 i1="11" i2="X" l="SPA"><s0>DNA</s0>
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<s4>CD</s4>
<s5>96</s5>
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<fC03 i1="12" i2="X" l="ENG"><s0>Genetic diversity</s0>
<s4>CD</s4>
<s5>96</s5>
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<s4>CD</s4>
<s5>96</s5>
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<s2>NS</s2>
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<s2>NS</s2>
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<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s2>NS</s2>
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<s5>33</s5>
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<s5>33</s5>
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<fC07 i1="06" i2="X" l="FRE"><s0>Biologie moléculaire</s0>
<s5>34</s5>
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<s5>34</s5>
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<s5>34</s5>
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<s5>35</s5>
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<s5>35</s5>
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<s5>35</s5>
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<s5>40</s5>
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<s5>40</s5>
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<s5>40</s5>
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<s5>41</s5>
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<fC07 i1="09" i2="X" l="ENG"><s0>Fruit crop</s0>
<s5>41</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Planta frutal</s0>
<s5>41</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Agrume</s0>
<s5>42</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>42</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Agrios</s0>
<s5>42</s5>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Acide nucléique</s0>
<s5>50</s5>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Nucleic acid</s0>
<s5>50</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Acido nucleico</s0>
<s5>50</s5>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Diversité</s0>
<s5>64</s5>
</fC07>
<fC07 i1="12" i2="X" l="ENG"><s0>Diversity</s0>
<s5>64</s5>
</fC07>
<fC07 i1="12" i2="X" l="SPA"><s0>Diversidad</s0>
<s5>64</s5>
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<server><NO>PASCAL 95-0500201 INIST</NO>
<ET>DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus</ET>
<AU>LURO (F.); LAIGRET (F.); BOVE (J.-M.); OLLITRAULT (P.)</AU>
<AF>INRA, cent. coopération internationale rech. agronomique développement-fruits légumes horticoles, stn. rech. agronomique Corse/20230 San Nicolao/France (1 aut.); INRA Univ. Bordaux II, lab. biologie cellulaire moléculaire/33883 Villenave d'Ornon/France (2 aut., 3 aut.); cent. coopération internationale rech. agronomique développement-fruits légumes horticoles/34032 Montpellier/France (4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>HortScience; ISSN 0018-5345; Coden HJHSAR; Etats-Unis; Da. 1995; Vol. 30; No. 5; Pp. 1063-1067; Bibl. 27 ref.</SO>
<LA>Anglais</LA>
<EA>We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L.) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique for discriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 C. reticulata cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related.</EA>
<CC>002A32D01D4</CC>
<FD>Etude en laboratoire; Variabilité génétique; Séquence répétée; Méthode fingerprint; Amplification génique; Réaction chaîne polymérase; Cultivar; Citrus volkameriana; Citrus sinensis; Citrus reticulata; DNA; Diversité génétique</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Amélioration génétique; Biologie moléculaire; Méthode analyse; Arbre fruitier; Plante fruitière; Agrume; Acide nucléique; Diversité</FG>
<ED>Laboratory study; Genetic variability; Repeated sequence; Fingerprint method; Gene amplification; Polymerase chain reaction; Cultivar; Citrus volkameriana; Citrus sinensis; Citrus reticulata; DNA; Genetic diversity</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Genetic improvement; Molecular biology; Analysis method; Fruit tree; Fruit crop; Citrus fruit; Nucleic acid; Diversity</EG>
<SD>Estudio en laboratorio; Variabilidad genética; Secuencia repetida; Método fingerprint; Amplificación génica; Reacción cadena polimerasa; Cultivar; Citrus volkameriana; Citrus sinensis; Citrus reticulata; DNA; Diversidád genética</SD>
<LO>INIST-13300.354000053922700380</LO>
<ID>95-0500201</ID>
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