Characterization and regulation of ammonium transport systems in Citrus plants
Identifieur interne : 000851 ( PascalFrancis/Corpus ); précédent : 000850; suivant : 000852Characterization and regulation of ammonium transport systems in Citrus plants
Auteurs : M. Cerezo ; P. Tillard ; A. Gojon ; E. Primo-Millo ; P. Garcia-AgustinSource :
- Planta [ 0032-0935 ] ; 2001.
Descripteurs français
- Pascal (Inist)
- Caractérisation, Régulation, Transport biologique, Marquage isotopique, Protéine transport, Site haute affinité, Absorption, Site faible affinité, Nutrition, Ammonium, Nitrate, Proton, Déficit, Métabolisme énergétique, Gradient proton, Gradient concentration, Racine, Citrus sinensis Poncirus trifoliata, Azote 15.
English descriptors
- KwdEn :
Abstract
We have investigated both the kinetics and regulation of 15NH4+ influx in roots of 3-month-old hydroponically grown Citrus (Citrus sinensis L. Osbeck x Poncirus trifoliata Blanco) seedlings. The 15NH4+ influx is saturable below an external ammonium concentration of 1 mM, indicating the action of a high-affinity transport system (HATS). The HATS is under feedback repression by the N status of the plant, being downregulated in plants adequately supplied with N during growth, and up-regulated by N-starvation. When assayed between 1 and 50 mM [15NH4+]0, the 15NH4+ influx showed a linear response typical of a low-affinity transport system (LATS). The activity of the LATS increased in plants supplied with NH4+ as compared with plants grown on an N-free medium. Transfer of the plants to N-free solution resulted in a marked decrease in the LATS-mediated 15NH4+ influx. Accordingly, resupply of NH4+ after N-starvation triggered a dramatic stimulation of the activity of the LATS. These data provide evidence that in Citrus plants, the LATS or at least one of its components is inducible by NH4+. Even when up-regulated, both the HATS and the LATS displayed a limited capacity, as compared with that usually found in herbaceous species. The use of various metabolic uncouplers or inhibitors indicated that 15NH4+ influx mediated by the HATS is strongly dependent on energy metabolism and H transmembrane electrochemical gradient. By contrast, the LATS is not affected by protonophores or inhibitors of the H+-ATPase, suggesting that its activity is mostly driven by the NH4+/NH3 transmembrane gradient. In agreement with these hypotheses, the HATS-mediated 15NH4+ influx was strongly inhibited when the solution pH was raised from 4 to 7, whereas influx mediated by the LATS was slightly stimulated.
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Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 02-0149830 INIST |
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ET : | Characterization and regulation of ammonium transport systems in Citrus plants |
AU : | CEREZO (M.); TILLARD (P.); GOJON (A.); PRIMO-MILLO (E.); GARCIA-AGUSTIN (P.) |
AF : | Unidad de Fisiología Vegetal, Departamento de Ciencias Expérimentales, Escuela Superior de Tecnología y Ciencias Experimentales, Universitat Jaume I/12071 Castellón/Espagne (1 aut., 5 aut.); Biochimie et Physiologie Moléculaire des plantes, UMR 5004, Agro-M, CNRS, INRA, UM2, Place Viala/34060 Montpellier/France (2 aut., 3 aut.); Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial/46113 Moncada Valencia/Espagne (4 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Planta; ISSN 0032-0935; Coden PLANAB; Allemagne; Da. 2001; Vol. 214; No. 1; Pp. 97-105; Bibl. 33 ref. |
LA : | Anglais |
EA : | We have investigated both the kinetics and regulation of 15NH4+ influx in roots of 3-month-old hydroponically grown Citrus (Citrus sinensis L. Osbeck x Poncirus trifoliata Blanco) seedlings. The 15NH4+ influx is saturable below an external ammonium concentration of 1 mM, indicating the action of a high-affinity transport system (HATS). The HATS is under feedback repression by the N status of the plant, being downregulated in plants adequately supplied with N during growth, and up-regulated by N-starvation. When assayed between 1 and 50 mM [15NH4+]0, the 15NH4+ influx showed a linear response typical of a low-affinity transport system (LATS). The activity of the LATS increased in plants supplied with NH4+ as compared with plants grown on an N-free medium. Transfer of the plants to N-free solution resulted in a marked decrease in the LATS-mediated 15NH4+ influx. Accordingly, resupply of NH4+ after N-starvation triggered a dramatic stimulation of the activity of the LATS. These data provide evidence that in Citrus plants, the LATS or at least one of its components is inducible by NH4+. Even when up-regulated, both the HATS and the LATS displayed a limited capacity, as compared with that usually found in herbaceous species. The use of various metabolic uncouplers or inhibitors indicated that 15NH4+ influx mediated by the HATS is strongly dependent on energy metabolism and H transmembrane electrochemical gradient. By contrast, the LATS is not affected by protonophores or inhibitors of the H+-ATPase, suggesting that its activity is mostly driven by the NH4+/NH3 transmembrane gradient. In agreement with these hypotheses, the HATS-mediated 15NH4+ influx was strongly inhibited when the solution pH was raised from 4 to 7, whereas influx mediated by the LATS was slightly stimulated. |
CC : | 002A10C; 002A32E05C |
FD : | Caractérisation; Régulation; Transport biologique; Marquage isotopique; Protéine transport; Site haute affinité; Absorption; Site faible affinité; Nutrition; Ammonium; Nitrate; Proton; Déficit; Métabolisme énergétique; Gradient proton; Gradient concentration; Racine; Citrus sinensis Poncirus trifoliata; Azote 15 |
FG : | Agrume; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Elément minéral; Nutriment; Traceur; Azote Isotope |
ED : | Characterization; Regulation(control); Biological transport; Isotope labelling; Carrier protein; High affinity site; Absorption; Low affinity site; Nutrition; Ammonium; Nitrates; Proton; Deficiency; Energy metabolism; Proton gradient; Concentration gradient; Root |
EG : | Citrus fruit; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Inorganic element; Nutrient; Tracers; Nitrogen Isotopes |
SD : | Caracterización; Regulación; Transporte biológico; Marcación isotópica; Proteína transportador; Sitio alta afinidad; Absorción; Sitio debil afinidad; Nutrición; Amonio; Nitrato; Protón; Déficiencia; Metabolismo energético; Gradiente protón; Gradiente concentración; Raíz |
LO : | INIST-916.354000102199970120 |
ID : | 02-0149830 |
Links to Exploration step
Pascal:02-0149830Le document en format XML
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<term>Deficiency</term>
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<term>Ammonium</term>
<term>Nitrate</term>
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<term>Métabolisme énergétique</term>
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<front><div type="abstract" xml:lang="en">We have investigated both the kinetics and regulation of <sup>15</sup>
NH<sup>4+</sup>
influx in roots of 3-month-old hydroponically grown Citrus (Citrus sinensis L. Osbeck x Poncirus trifoliata Blanco) seedlings. The <sup>15</sup>
NH<sub>4</sub>
<sup>+</sup>
influx is saturable below an external ammonium concentration of 1 mM, indicating the action of a high-affinity transport system (HATS). The HATS is under feedback repression by the N status of the plant, being downregulated in plants adequately supplied with N during growth, and up-regulated by N-starvation. When assayed between 1 and 50 mM [<sup>15</sup>
NH<sub>4</sub>
<sup>+</sup>
]<sub>0</sub>
, the 15NH4+ influx showed a linear response typical of a low-affinity transport system (LATS). The activity of the LATS increased in plants supplied with NH<sub>4</sub>
<sup>+</sup>
as compared with plants grown on an N-free medium. Transfer of the plants to N-free solution resulted in a marked decrease in the LATS-mediated 15NH4+ influx. Accordingly, resupply of NH<sub>4</sub>
<sup>+</sup>
after N-starvation triggered a dramatic stimulation of the activity of the LATS. These data provide evidence that in Citrus plants, the LATS or at least one of its components is inducible by NH<sub>4</sub>
<sup>+</sup>
. Even when up-regulated, both the HATS and the LATS displayed a limited capacity, as compared with that usually found in herbaceous species. The use of various metabolic uncouplers or inhibitors indicated that 15NH4+ influx mediated by the HATS is strongly dependent on energy metabolism and H transmembrane electrochemical gradient. By contrast, the LATS is not affected by protonophores or inhibitors of the H<sup>+</sup>
-ATPase, suggesting that its activity is mostly driven by the NH<sub>4</sub>
<sup>+</sup>
/NH<sub>3</sub>
transmembrane gradient. In agreement with these hypotheses, the HATS-mediated 15NH4+ influx was strongly inhibited when the solution pH was raised from 4 to 7, whereas influx mediated by the LATS was slightly stimulated.</div>
</front>
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<fC01 i1="01" l="ENG"><s0>We have investigated both the kinetics and regulation of <sup>15</sup>
NH<sup>4+</sup>
influx in roots of 3-month-old hydroponically grown Citrus (Citrus sinensis L. Osbeck x Poncirus trifoliata Blanco) seedlings. The <sup>15</sup>
NH<sub>4</sub>
<sup>+</sup>
influx is saturable below an external ammonium concentration of 1 mM, indicating the action of a high-affinity transport system (HATS). The HATS is under feedback repression by the N status of the plant, being downregulated in plants adequately supplied with N during growth, and up-regulated by N-starvation. When assayed between 1 and 50 mM [<sup>15</sup>
NH<sub>4</sub>
<sup>+</sup>
]<sub>0</sub>
, the 15NH4+ influx showed a linear response typical of a low-affinity transport system (LATS). The activity of the LATS increased in plants supplied with NH<sub>4</sub>
<sup>+</sup>
as compared with plants grown on an N-free medium. Transfer of the plants to N-free solution resulted in a marked decrease in the LATS-mediated 15NH4+ influx. Accordingly, resupply of NH<sub>4</sub>
<sup>+</sup>
after N-starvation triggered a dramatic stimulation of the activity of the LATS. These data provide evidence that in Citrus plants, the LATS or at least one of its components is inducible by NH<sub>4</sub>
<sup>+</sup>
. Even when up-regulated, both the HATS and the LATS displayed a limited capacity, as compared with that usually found in herbaceous species. The use of various metabolic uncouplers or inhibitors indicated that 15NH4+ influx mediated by the HATS is strongly dependent on energy metabolism and H transmembrane electrochemical gradient. By contrast, the LATS is not affected by protonophores or inhibitors of the H<sup>+</sup>
-ATPase, suggesting that its activity is mostly driven by the NH<sub>4</sub>
<sup>+</sup>
/NH<sub>3</sub>
transmembrane gradient. In agreement with these hypotheses, the HATS-mediated 15NH4+ influx was strongly inhibited when the solution pH was raised from 4 to 7, whereas influx mediated by the LATS was slightly stimulated.</s0>
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<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Ammonium</s0>
<s2>NC</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Ammonium</s0>
<s2>NC</s2>
<s5>15</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Amonio</s0>
<s2>NC</s2>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Nitrate</s0>
<s2>NA</s2>
<s2>FX</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Nitrates</s0>
<s2>NA</s2>
<s2>FX</s2>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Nitrato</s0>
<s2>NA</s2>
<s2>FX</s2>
<s5>16</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Proton</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Proton</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Protón</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Déficit</s0>
<s5>33</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Deficiency</s0>
<s5>33</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Déficiencia</s0>
<s5>33</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Métabolisme énergétique</s0>
<s5>34</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Energy metabolism</s0>
<s5>34</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Metabolismo energético</s0>
<s5>34</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Gradient proton</s0>
<s5>35</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Proton gradient</s0>
<s5>35</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Gradiente protón</s0>
<s5>35</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Gradient concentration</s0>
<s5>36</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Concentration gradient</s0>
<s5>36</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Gradiente concentración</s0>
<s5>36</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Racine</s0>
<s5>37</s5>
</fC03>
<fC03 i1="17" i2="X" l="ENG"><s0>Root</s0>
<s5>37</s5>
</fC03>
<fC03 i1="17" i2="X" l="SPA"><s0>Raíz</s0>
<s5>37</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Citrus sinensis Poncirus trifoliata</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>77</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>Azote 15</s0>
<s2>FF</s2>
<s4>INC</s4>
<s5>81</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
<s5>40</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
<s5>40</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
<s5>40</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Elément minéral</s0>
<s5>50</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Inorganic element</s0>
<s5>50</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Elemento inorgánico</s0>
<s5>50</s5>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Nutriment</s0>
<s5>51</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Nutrient</s0>
<s5>51</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Nutriente</s0>
<s5>51</s5>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Traceur</s0>
<s5>53</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Tracers</s0>
<s5>53</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Trazador</s0>
<s5>53</s5>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Azote Isotope</s0>
<s2>NC</s2>
<s2>NA</s2>
<s5>54</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Nitrogen Isotopes</s0>
<s2>NC</s2>
<s2>NA</s2>
<s5>54</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Nitrógeno Isótopo</s0>
<s2>NC</s2>
<s2>NA</s2>
<s5>54</s5>
</fC07>
<fN21><s1>084</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 02-0149830 INIST</NO>
<ET>Characterization and regulation of ammonium transport systems in Citrus plants</ET>
<AU>CEREZO (M.); TILLARD (P.); GOJON (A.); PRIMO-MILLO (E.); GARCIA-AGUSTIN (P.)</AU>
<AF>Unidad de Fisiología Vegetal, Departamento de Ciencias Expérimentales, Escuela Superior de Tecnología y Ciencias Experimentales, Universitat Jaume I/12071 Castellón/Espagne (1 aut., 5 aut.); Biochimie et Physiologie Moléculaire des plantes, UMR 5004, Agro-M, CNRS, INRA, UM2, Place Viala/34060 Montpellier/France (2 aut., 3 aut.); Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial/46113 Moncada Valencia/Espagne (4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Planta; ISSN 0032-0935; Coden PLANAB; Allemagne; Da. 2001; Vol. 214; No. 1; Pp. 97-105; Bibl. 33 ref.</SO>
<LA>Anglais</LA>
<EA>We have investigated both the kinetics and regulation of <sup>15</sup>
NH<sup>4+</sup>
influx in roots of 3-month-old hydroponically grown Citrus (Citrus sinensis L. Osbeck x Poncirus trifoliata Blanco) seedlings. The <sup>15</sup>
NH<sub>4</sub>
<sup>+</sup>
influx is saturable below an external ammonium concentration of 1 mM, indicating the action of a high-affinity transport system (HATS). The HATS is under feedback repression by the N status of the plant, being downregulated in plants adequately supplied with N during growth, and up-regulated by N-starvation. When assayed between 1 and 50 mM [<sup>15</sup>
NH<sub>4</sub>
<sup>+</sup>
]<sub>0</sub>
, the 15NH4+ influx showed a linear response typical of a low-affinity transport system (LATS). The activity of the LATS increased in plants supplied with NH<sub>4</sub>
<sup>+</sup>
as compared with plants grown on an N-free medium. Transfer of the plants to N-free solution resulted in a marked decrease in the LATS-mediated 15NH4+ influx. Accordingly, resupply of NH<sub>4</sub>
<sup>+</sup>
after N-starvation triggered a dramatic stimulation of the activity of the LATS. These data provide evidence that in Citrus plants, the LATS or at least one of its components is inducible by NH<sub>4</sub>
<sup>+</sup>
. Even when up-regulated, both the HATS and the LATS displayed a limited capacity, as compared with that usually found in herbaceous species. The use of various metabolic uncouplers or inhibitors indicated that 15NH4+ influx mediated by the HATS is strongly dependent on energy metabolism and H transmembrane electrochemical gradient. By contrast, the LATS is not affected by protonophores or inhibitors of the H<sup>+</sup>
-ATPase, suggesting that its activity is mostly driven by the NH<sub>4</sub>
<sup>+</sup>
/NH<sub>3</sub>
transmembrane gradient. In agreement with these hypotheses, the HATS-mediated 15NH4+ influx was strongly inhibited when the solution pH was raised from 4 to 7, whereas influx mediated by the LATS was slightly stimulated.</EA>
<CC>002A10C; 002A32E05C</CC>
<FD>Caractérisation; Régulation; Transport biologique; Marquage isotopique; Protéine transport; Site haute affinité; Absorption; Site faible affinité; Nutrition; Ammonium; Nitrate; Proton; Déficit; Métabolisme énergétique; Gradient proton; Gradient concentration; Racine; Citrus sinensis Poncirus trifoliata; Azote 15</FD>
<FG>Agrume; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Elément minéral; Nutriment; Traceur; Azote Isotope</FG>
<ED>Characterization; Regulation(control); Biological transport; Isotope labelling; Carrier protein; High affinity site; Absorption; Low affinity site; Nutrition; Ammonium; Nitrates; Proton; Deficiency; Energy metabolism; Proton gradient; Concentration gradient; Root</ED>
<EG>Citrus fruit; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Inorganic element; Nutrient; Tracers; Nitrogen Isotopes</EG>
<SD>Caracterización; Regulación; Transporte biológico; Marcación isotópica; Proteína transportador; Sitio alta afinidad; Absorción; Sitio debil afinidad; Nutrición; Amonio; Nitrato; Protón; Déficiencia; Metabolismo energético; Gradiente protón; Gradiente concentración; Raíz</SD>
<LO>INIST-916.354000102199970120</LO>
<ID>02-0149830</ID>
</server>
</inist>
</record>
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