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Characterization of pinalate, a novel Citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content

Identifieur interne : 000777 ( PascalFrancis/Corpus ); précédent : 000776; suivant : 000778

Characterization of pinalate, a novel Citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content

Auteurs : Maria-Jesus Rodrigo ; José F. Marcos ; Fernando Alferez ; M. Dolores Mallent ; Lorenzo Zacarias

Source :

RBID : Pascal:03-0181254

Descripteurs français

English descriptors

Abstract

The characterization of a novel mutant, named Pinalate, derived from the orange (Citrus sinensis L. Osbeck) Navelate, which produces distinctive yellow fruits instead of the typical bright orange colouration, is reported. The carotenoid content and composition, and ABA content in leaf and flavedo tissue (coloured part of the skin) of fruits at different developmental and maturation stages were analysed. No important differences in leaf carotenoid pattern of both phenotypes were found. However, an unusual accumulation of linear carotenes (phytoene, phytofluene and ζ-carotene) was detected in the flavedo of Pinalate. As fruit maturation progressed, the flavedo of mutant fruit accumulated high amounts of these carotenes and the proportion of cyclic and oxygenated carotenoids was substantially lower than in the parental line. Full-coloured fruit of Pinalate contained about 44% phytoene, 21% phytofluene, 25% ζ-carotene, and 10% of xanthophylls, whereas, in Navelate, 98% of total carotenoids were xanthophylls and apocarotenoids. The ABA content in the flavedo of Pinalate mature fruit was 3-6 times lower than in the corresponding tissue of Navelate, while no differences were found in leaves. Other maturation processes were not affected in Pinalate fruit. Taken together, the results indicate that Pinalate is a fruit-specific alteration defective in ζ-carotene desaturase or in ζ-carotene desaturase-associated factors. Possible mechanisms responsible for the Pinalate phenotype are discussed. Because of the abnormal fruit-specific carotenoid complement and ABA deficiency, Pinalate may constitute an excellent system for the study of carotenogenesis in Citrus and the involvement of ABA in fruit maturation and stress responses.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A03   1    @0 J. Exp. Bot.
A05       @2 54
A06       @2 383
A08 01  1  ENG  @1 Characterization of pinalate, a novel Citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content
A11 01  1    @1 RODRIGO (Maria-Jesus)
A11 02  1    @1 MARCOS (José F.)
A11 03  1    @1 ALFEREZ (Fernando)
A11 04  1    @1 MALLENT (M. Dolores)
A11 05  1    @1 ZACARIAS (Lorenzo)
A14 01      @1 Departamento de Ciencia de Alimentos, Instituto de Agroquímica y Tecnologia de Alimentos (IATA)-CSIC, Apartado de Correos 73, Burjassot @2 46100 Valencia @3 ESP @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut.
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A21       @1 2003
A23 01      @0 ENG
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A44       @0 0000 @1 © 2003 INIST-CNRS. All rights reserved.
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A60       @1 P @3 PR
A61       @0 A
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C01 01    ENG  @0 The characterization of a novel mutant, named Pinalate, derived from the orange (Citrus sinensis L. Osbeck) Navelate, which produces distinctive yellow fruits instead of the typical bright orange colouration, is reported. The carotenoid content and composition, and ABA content in leaf and flavedo tissue (coloured part of the skin) of fruits at different developmental and maturation stages were analysed. No important differences in leaf carotenoid pattern of both phenotypes were found. However, an unusual accumulation of linear carotenes (phytoene, phytofluene and ζ-carotene) was detected in the flavedo of Pinalate. As fruit maturation progressed, the flavedo of mutant fruit accumulated high amounts of these carotenes and the proportion of cyclic and oxygenated carotenoids was substantially lower than in the parental line. Full-coloured fruit of Pinalate contained about 44% phytoene, 21% phytofluene, 25% ζ-carotene, and 10% of xanthophylls, whereas, in Navelate, 98% of total carotenoids were xanthophylls and apocarotenoids. The ABA content in the flavedo of Pinalate mature fruit was 3-6 times lower than in the corresponding tissue of Navelate, while no differences were found in leaves. Other maturation processes were not affected in Pinalate fruit. Taken together, the results indicate that Pinalate is a fruit-specific alteration defective in ζ-carotene desaturase or in ζ-carotene desaturase-associated factors. Possible mechanisms responsible for the Pinalate phenotype are discussed. Because of the abnormal fruit-specific carotenoid complement and ABA deficiency, Pinalate may constitute an excellent system for the study of carotenogenesis in Citrus and the involvement of ABA in fruit maturation and stress responses.
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N21       @1 104
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Format Inist (serveur)

NO : PASCAL 03-0181254 INIST
ET : Characterization of pinalate, a novel Citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content
AU : RODRIGO (Maria-Jesus); MARCOS (José F.); ALFEREZ (Fernando); MALLENT (M. Dolores); ZACARIAS (Lorenzo)
AF : Departamento de Ciencia de Alimentos, Instituto de Agroquímica y Tecnologia de Alimentos (IATA)-CSIC, Apartado de Correos 73, Burjassot/46100 Valencia/Espagne (1 aut., 2 aut., 3 aut., 4 aut., 5 aut.)
DT : Publication en série; Papier de recherche; Niveau analytique
SO : Journal of Experimental Botany; ISSN 0022-0957; Coden JEBOA6; Royaume-Uni; Da. 2003; Vol. 54; No. 383; Pp. 727-738; Bibl. 1 p.1/4
LA : Anglais
EA : The characterization of a novel mutant, named Pinalate, derived from the orange (Citrus sinensis L. Osbeck) Navelate, which produces distinctive yellow fruits instead of the typical bright orange colouration, is reported. The carotenoid content and composition, and ABA content in leaf and flavedo tissue (coloured part of the skin) of fruits at different developmental and maturation stages were analysed. No important differences in leaf carotenoid pattern of both phenotypes were found. However, an unusual accumulation of linear carotenes (phytoene, phytofluene and ζ-carotene) was detected in the flavedo of Pinalate. As fruit maturation progressed, the flavedo of mutant fruit accumulated high amounts of these carotenes and the proportion of cyclic and oxygenated carotenoids was substantially lower than in the parental line. Full-coloured fruit of Pinalate contained about 44% phytoene, 21% phytofluene, 25% ζ-carotene, and 10% of xanthophylls, whereas, in Navelate, 98% of total carotenoids were xanthophylls and apocarotenoids. The ABA content in the flavedo of Pinalate mature fruit was 3-6 times lower than in the corresponding tissue of Navelate, while no differences were found in leaves. Other maturation processes were not affected in Pinalate fruit. Taken together, the results indicate that Pinalate is a fruit-specific alteration defective in ζ-carotene desaturase or in ζ-carotene desaturase-associated factors. Possible mechanisms responsible for the Pinalate phenotype are discussed. Because of the abnormal fruit-specific carotenoid complement and ABA deficiency, Pinalate may constitute an excellent system for the study of carotenogenesis in Citrus and the involvement of ABA in fruit maturation and stress responses.
CC : 002A32E06E; 002A10F05
FD : Mutation; Fruit; Coloration; Accumulation biologique; Caroténoïde; Composition chimique; Xanthophylle; Abscissique acide; Déficit; Citrus sinensis; Carotene 7,8-desaturase; Phénotype
FG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Oxidoreductases; Enzyme; Agrume
ED : Mutation; Fruit; Coloration; Biological accumulation; Carotenoid; Chemical composition; Xanthophyll; Abscisic acid; Deficiency; Citrus sinensis; Carotene 7,8-desaturase; Phenotype
EG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Oxidoreductases; Enzyme; Citrus fruit
SD : Mutación; Fruto; Coloración; Acumulación biológica; Carotenoide; Composición química; Xantófilo; Abscísico ácido; Déficiencia; Citrus sinensis; Carotene 7,8-desaturase; Fenotipo
LO : INIST-6923.354000107698190130
ID : 03-0181254

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Pascal:03-0181254

Le document en format XML

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<div type="abstract" xml:lang="en">The characterization of a novel mutant, named Pinalate, derived from the orange (Citrus sinensis L. Osbeck) Navelate, which produces distinctive yellow fruits instead of the typical bright orange colouration, is reported. The carotenoid content and composition, and ABA content in leaf and flavedo tissue (coloured part of the skin) of fruits at different developmental and maturation stages were analysed. No important differences in leaf carotenoid pattern of both phenotypes were found. However, an unusual accumulation of linear carotenes (phytoene, phytofluene and ζ-carotene) was detected in the flavedo of Pinalate. As fruit maturation progressed, the flavedo of mutant fruit accumulated high amounts of these carotenes and the proportion of cyclic and oxygenated carotenoids was substantially lower than in the parental line. Full-coloured fruit of Pinalate contained about 44% phytoene, 21% phytofluene, 25% ζ-carotene, and 10% of xanthophylls, whereas, in Navelate, 98% of total carotenoids were xanthophylls and apocarotenoids. The ABA content in the flavedo of Pinalate mature fruit was 3-6 times lower than in the corresponding tissue of Navelate, while no differences were found in leaves. Other maturation processes were not affected in Pinalate fruit. Taken together, the results indicate that Pinalate is a fruit-specific alteration defective in ζ-carotene desaturase or in ζ-carotene desaturase-associated factors. Possible mechanisms responsible for the Pinalate phenotype are discussed. Because of the abnormal fruit-specific carotenoid complement and ABA deficiency, Pinalate may constitute an excellent system for the study of carotenogenesis in Citrus and the involvement of ABA in fruit maturation and stress responses.</div>
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<sZ>5 aut.</sZ>
</fA14>
<fA20>
<s1>727-738</s1>
</fA20>
<fA21>
<s1>2003</s1>
</fA21>
<fA23 i1="01">
<s0>ENG</s0>
</fA23>
<fA43 i1="01">
<s1>INIST</s1>
<s2>6923</s2>
<s5>354000107698190130</s5>
</fA43>
<fA44>
<s0>0000</s0>
<s1>© 2003 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45>
<s0>1 p.1/4</s0>
</fA45>
<fA47 i1="01" i2="1">
<s0>03-0181254</s0>
</fA47>
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<s1>P</s1>
<s3>PR</s3>
</fA60>
<fA61>
<s0>A</s0>
</fA61>
<fA64 i1="01" i2="1">
<s0>Journal of Experimental Botany</s0>
</fA64>
<fA66 i1="01">
<s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG">
<s0>The characterization of a novel mutant, named Pinalate, derived from the orange (Citrus sinensis L. Osbeck) Navelate, which produces distinctive yellow fruits instead of the typical bright orange colouration, is reported. The carotenoid content and composition, and ABA content in leaf and flavedo tissue (coloured part of the skin) of fruits at different developmental and maturation stages were analysed. No important differences in leaf carotenoid pattern of both phenotypes were found. However, an unusual accumulation of linear carotenes (phytoene, phytofluene and ζ-carotene) was detected in the flavedo of Pinalate. As fruit maturation progressed, the flavedo of mutant fruit accumulated high amounts of these carotenes and the proportion of cyclic and oxygenated carotenoids was substantially lower than in the parental line. Full-coloured fruit of Pinalate contained about 44% phytoene, 21% phytofluene, 25% ζ-carotene, and 10% of xanthophylls, whereas, in Navelate, 98% of total carotenoids were xanthophylls and apocarotenoids. The ABA content in the flavedo of Pinalate mature fruit was 3-6 times lower than in the corresponding tissue of Navelate, while no differences were found in leaves. Other maturation processes were not affected in Pinalate fruit. Taken together, the results indicate that Pinalate is a fruit-specific alteration defective in ζ-carotene desaturase or in ζ-carotene desaturase-associated factors. Possible mechanisms responsible for the Pinalate phenotype are discussed. Because of the abnormal fruit-specific carotenoid complement and ABA deficiency, Pinalate may constitute an excellent system for the study of carotenogenesis in Citrus and the involvement of ABA in fruit maturation and stress responses.</s0>
</fC01>
<fC02 i1="01" i2="X">
<s0>002A32E06E</s0>
</fC02>
<fC02 i1="02" i2="X">
<s0>002A10F05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE">
<s0>Mutation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG">
<s0>Mutation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA">
<s0>Mutación</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Fruit</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Fruit</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Fruto</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Coloration</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Coloration</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Coloración</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Accumulation biologique</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Biological accumulation</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Acumulación biológica</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Caroténoïde</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Carotenoid</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Carotenoide</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Composition chimique</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>Chemical composition</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>Composición química</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE">
<s0>Xanthophylle</s0>
<s2>NK</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Xanthophyll</s0>
<s2>NK</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
<s0>Xantófilo</s0>
<s2>NK</s2>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE">
<s0>Abscissique acide</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG">
<s0>Abscisic acid</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
<s0>Abscísico ácido</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE">
<s0>Déficit</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG">
<s0>Deficiency</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA">
<s0>Déficiencia</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE">
<s0>Carotene 7,8-desaturase</s0>
<s2>FE</s2>
<s5>33</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG">
<s0>Carotene 7,8-desaturase</s0>
<s2>FE</s2>
<s5>33</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA">
<s0>Carotene 7,8-desaturase</s0>
<s2>FE</s2>
<s5>33</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE">
<s0>Phénotype</s0>
<s5>34</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG">
<s0>Phenotype</s0>
<s5>34</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA">
<s0>Fenotipo</s0>
<s5>34</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Enzyme</s0>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Enzyme</s0>
</fC07>
<fC07 i1="06" i2="X" l="SPA">
<s0>Enzima</s0>
</fC07>
<fC07 i1="07" i2="X" l="FRE">
<s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG">
<s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA">
<s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fN21>
<s1>104</s1>
</fN21>
<fN82>
<s1>PSI</s1>
</fN82>
</pA>
</standard>
<server>
<NO>PASCAL 03-0181254 INIST</NO>
<ET>Characterization of pinalate, a novel Citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content</ET>
<AU>RODRIGO (Maria-Jesus); MARCOS (José F.); ALFEREZ (Fernando); MALLENT (M. Dolores); ZACARIAS (Lorenzo)</AU>
<AF>Departamento de Ciencia de Alimentos, Instituto de Agroquímica y Tecnologia de Alimentos (IATA)-CSIC, Apartado de Correos 73, Burjassot/46100 Valencia/Espagne (1 aut., 2 aut., 3 aut., 4 aut., 5 aut.)</AF>
<DT>Publication en série; Papier de recherche; Niveau analytique</DT>
<SO>Journal of Experimental Botany; ISSN 0022-0957; Coden JEBOA6; Royaume-Uni; Da. 2003; Vol. 54; No. 383; Pp. 727-738; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>The characterization of a novel mutant, named Pinalate, derived from the orange (Citrus sinensis L. Osbeck) Navelate, which produces distinctive yellow fruits instead of the typical bright orange colouration, is reported. The carotenoid content and composition, and ABA content in leaf and flavedo tissue (coloured part of the skin) of fruits at different developmental and maturation stages were analysed. No important differences in leaf carotenoid pattern of both phenotypes were found. However, an unusual accumulation of linear carotenes (phytoene, phytofluene and ζ-carotene) was detected in the flavedo of Pinalate. As fruit maturation progressed, the flavedo of mutant fruit accumulated high amounts of these carotenes and the proportion of cyclic and oxygenated carotenoids was substantially lower than in the parental line. Full-coloured fruit of Pinalate contained about 44% phytoene, 21% phytofluene, 25% ζ-carotene, and 10% of xanthophylls, whereas, in Navelate, 98% of total carotenoids were xanthophylls and apocarotenoids. The ABA content in the flavedo of Pinalate mature fruit was 3-6 times lower than in the corresponding tissue of Navelate, while no differences were found in leaves. Other maturation processes were not affected in Pinalate fruit. Taken together, the results indicate that Pinalate is a fruit-specific alteration defective in ζ-carotene desaturase or in ζ-carotene desaturase-associated factors. Possible mechanisms responsible for the Pinalate phenotype are discussed. Because of the abnormal fruit-specific carotenoid complement and ABA deficiency, Pinalate may constitute an excellent system for the study of carotenogenesis in Citrus and the involvement of ABA in fruit maturation and stress responses.</EA>
<CC>002A32E06E; 002A10F05</CC>
<FD>Mutation; Fruit; Coloration; Accumulation biologique; Caroténoïde; Composition chimique; Xanthophylle; Abscissique acide; Déficit; Citrus sinensis; Carotene 7,8-desaturase; Phénotype</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Oxidoreductases; Enzyme; Agrume</FG>
<ED>Mutation; Fruit; Coloration; Biological accumulation; Carotenoid; Chemical composition; Xanthophyll; Abscisic acid; Deficiency; Citrus sinensis; Carotene 7,8-desaturase; Phenotype</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Oxidoreductases; Enzyme; Citrus fruit</EG>
<SD>Mutación; Fruto; Coloración; Acumulación biológica; Carotenoide; Composición química; Xantófilo; Abscísico ácido; Déficiencia; Citrus sinensis; Carotene 7,8-desaturase; Fenotipo</SD>
<LO>INIST-6923.354000107698190130</LO>
<ID>03-0181254</ID>
</server>
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