Separation and characterization of a salt-dependent pectin methylesterase from Citrus sinensis Var. valencia fruit tissue
Identifieur interne : 000755 ( PascalFrancis/Corpus ); précédent : 000754; suivant : 000756Separation and characterization of a salt-dependent pectin methylesterase from Citrus sinensis Var. valencia fruit tissue
Auteurs : Randall G. Cameron ; Brett J. Savary ; Arland T. Hotchkiss ; Marshall L. Fishman ; H. O. A. K. Chau ; Robert A. Baker ; Karel GrohmannSource :
- Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2003.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
A pectin methylesterase (PME) from sweet orange fruit rag tissue, which does not destabilize citrus juice cloud, has been characterized. It is a salt-dependent PME (type II) and exhibits optimal activity between 0.1 and 0.2 M NaCI at pH 7.5. The pH optimum shifted to a more alkaline range as the salt molarity decreased (pH 8.5-9.5 at 50 mM NaCI). It has an apparent molecular mass of 32.4 kDa as determined by gel filtration chromatography, an apparent molecular mass of 33.5 kDa as determined by denaturing electrophoresis, and a pl of 10.1 and exhibits a single activity band after isoelectric focusing (IEF). It has a Km of 0.0487 mg/mL and a Vmax of 4.2378 nkat/mg of protein on 59% DE citrus pectin. Deblocking the N-terminus revealed a partial peptide composed of SVTPNV. Deesterification of non-calcium-sensitive pectin by 6.5% increased the calcium-sensitive pectin ratio (CSPR) from 0. 045 ± 0.011 to 0. 829 ± 0.033 but had little, if any, effect on pectin molecular weight. These properties indicate this enzyme will be useful for studying the PME mode of action as it relates to juice cloud destabilization.
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Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 03-0344968 INIST |
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ET : | Separation and characterization of a salt-dependent pectin methylesterase from Citrus sinensis Var. valencia fruit tissue |
AU : | CAMERON (Randall G.); SAVARY (Brett J.); HOTCHKISS (Arland T.); FISHMAN (Marshall L.); CHAU (H. O. A. K.); BAKER (Robert A.); GROHMANN (Karel) |
AF : | Citrus and Subtropical Products Laboratory, South Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, 600 Avenue South N.W./Winter Haven, Florida 33881/Etats-Unis (1 aut.); Eastern Regional Research Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, 600 Mermaid Lane/Wyndmoor, Pennsylvania 19038/Etats-Unis (2 aut., 3 aut., 4 aut., 5 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of agricultural and food chemistry : (Print); ISSN 0021-8561; Coden JAFCAU; Etats-Unis; Da. 2003; Vol. 51; No. 7; Pp. 2070-2075; Bibl. 39 ref. |
LA : | Anglais |
EA : | A pectin methylesterase (PME) from sweet orange fruit rag tissue, which does not destabilize citrus juice cloud, has been characterized. It is a salt-dependent PME (type II) and exhibits optimal activity between 0.1 and 0.2 M NaCI at pH 7.5. The pH optimum shifted to a more alkaline range as the salt molarity decreased (pH 8.5-9.5 at 50 mM NaCI). It has an apparent molecular mass of 32.4 kDa as determined by gel filtration chromatography, an apparent molecular mass of 33.5 kDa as determined by denaturing electrophoresis, and a pl of 10.1 and exhibits a single activity band after isoelectric focusing (IEF). It has a Km of 0.0487 mg/mL and a Vmax of 4.2378 nkat/mg of protein on 59% DE citrus pectin. Deblocking the N-terminus revealed a partial peptide composed of SVTPNV. Deesterification of non-calcium-sensitive pectin by 6.5% increased the calcium-sensitive pectin ratio (CSPR) from 0. 045 ± 0.011 to 0. 829 ± 0.033 but had little, if any, effect on pectin molecular weight. These properties indicate this enzyme will be useful for studying the PME mode of action as it relates to juice cloud destabilization. |
CC : | 002A35B09 |
FD : | Caractérisation; Séparation; Pectinesterase; Sensibilité résistance; Activité enzymatique; Citrus sinensis; Sel; Galacturonique acide; Pectine |
FG : | Carboxylic ester hydrolases; Esterases; Hydrolases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Fruit; Polyoside; Biopolymère |
ED : | Characterization; Separation; Pectinesterase; Sensitivity resistance; Enzymatic activity; Citrus sinensis; Salt; Galacturonic acid; Pectin |
EG : | Carboxylic ester hydrolases; Esterases; Hydrolases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Fruit; Polysaccharide; Biopolymer |
SD : | Caracterización; Separación; Pectinesterase; Sensibilidad resistencia; Actividad enzimática; Citrus sinensis; Sal; Galacturónico ácido; Pectina |
LO : | INIST-7332.354000109555090500 |
ID : | 03-0344968 |
Links to Exploration step
Pascal:03-0344968Le document en format XML
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<term>Pectinesterase</term>
<term>Salt</term>
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<term>Separation</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Caractérisation</term>
<term>Séparation</term>
<term>Pectinesterase</term>
<term>Sensibilité résistance</term>
<term>Activité enzymatique</term>
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<front><div type="abstract" xml:lang="en">A pectin methylesterase (PME) from sweet orange fruit rag tissue, which does not destabilize citrus juice cloud, has been characterized. It is a salt-dependent PME (type II) and exhibits optimal activity between 0.1 and 0.2 M NaCI at pH 7.5. The pH optimum shifted to a more alkaline range as the salt molarity decreased (pH 8.5-9.5 at 50 mM NaCI). It has an apparent molecular mass of 32.4 kDa as determined by gel filtration chromatography, an apparent molecular mass of 33.5 kDa as determined by denaturing electrophoresis, and a pl of 10.1 and exhibits a single activity band after isoelectric focusing (IEF). It has a K<sub>m</sub>
of 0.0487 mg/mL and a V<sub>max</sub>
of 4.2378 nkat/mg of protein on 59% DE citrus pectin. Deblocking the N-terminus revealed a partial peptide composed of SVTPNV. Deesterification of non-calcium-sensitive pectin by 6.5% increased the calcium-sensitive pectin ratio (CSPR) from 0. 045 ± 0.011 to 0. 829 ± 0.033 but had little, if any, effect on pectin molecular weight. These properties indicate this enzyme will be useful for studying the PME mode of action as it relates to juice cloud destabilization.</div>
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<fC01 i1="01" l="ENG"><s0>A pectin methylesterase (PME) from sweet orange fruit rag tissue, which does not destabilize citrus juice cloud, has been characterized. It is a salt-dependent PME (type II) and exhibits optimal activity between 0.1 and 0.2 M NaCI at pH 7.5. The pH optimum shifted to a more alkaline range as the salt molarity decreased (pH 8.5-9.5 at 50 mM NaCI). It has an apparent molecular mass of 32.4 kDa as determined by gel filtration chromatography, an apparent molecular mass of 33.5 kDa as determined by denaturing electrophoresis, and a pl of 10.1 and exhibits a single activity band after isoelectric focusing (IEF). It has a K<sub>m</sub>
of 0.0487 mg/mL and a V<sub>max</sub>
of 4.2378 nkat/mg of protein on 59% DE citrus pectin. Deblocking the N-terminus revealed a partial peptide composed of SVTPNV. Deesterification of non-calcium-sensitive pectin by 6.5% increased the calcium-sensitive pectin ratio (CSPR) from 0. 045 ± 0.011 to 0. 829 ± 0.033 but had little, if any, effect on pectin molecular weight. These properties indicate this enzyme will be useful for studying the PME mode of action as it relates to juice cloud destabilization.</s0>
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<s2>FE</s2>
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<s2>FE</s2>
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<s2>FE</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Enzyme</s0>
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</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Agrume</s0>
<s5>32</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>32</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Agrios</s0>
<s5>32</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Fruit</s0>
<s5>33</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Fruit</s0>
<s5>33</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Fruto</s0>
<s5>33</s5>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Polyoside</s0>
<s2>NK</s2>
<s5>34</s5>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Polysaccharide</s0>
<s2>NK</s2>
<s5>34</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Poliósido</s0>
<s2>NK</s2>
<s5>34</s5>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Biopolymère</s0>
<s5>54</s5>
</fC07>
<fC07 i1="12" i2="X" l="ENG"><s0>Biopolymer</s0>
<s5>54</s5>
</fC07>
<fC07 i1="12" i2="X" l="SPA"><s0>Biopolímero</s0>
<s5>54</s5>
</fC07>
<fN21><s1>246</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 03-0344968 INIST</NO>
<ET>Separation and characterization of a salt-dependent pectin methylesterase from Citrus sinensis Var. valencia fruit tissue</ET>
<AU>CAMERON (Randall G.); SAVARY (Brett J.); HOTCHKISS (Arland T.); FISHMAN (Marshall L.); CHAU (H. O. A. K.); BAKER (Robert A.); GROHMANN (Karel)</AU>
<AF>Citrus and Subtropical Products Laboratory, South Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, 600 Avenue South N.W./Winter Haven, Florida 33881/Etats-Unis (1 aut.); Eastern Regional Research Center, North Atlantic Area, Agricultural Research Service, U.S. Department of Agriculture, 600 Mermaid Lane/Wyndmoor, Pennsylvania 19038/Etats-Unis (2 aut., 3 aut., 4 aut., 5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of agricultural and food chemistry : (Print); ISSN 0021-8561; Coden JAFCAU; Etats-Unis; Da. 2003; Vol. 51; No. 7; Pp. 2070-2075; Bibl. 39 ref.</SO>
<LA>Anglais</LA>
<EA>A pectin methylesterase (PME) from sweet orange fruit rag tissue, which does not destabilize citrus juice cloud, has been characterized. It is a salt-dependent PME (type II) and exhibits optimal activity between 0.1 and 0.2 M NaCI at pH 7.5. The pH optimum shifted to a more alkaline range as the salt molarity decreased (pH 8.5-9.5 at 50 mM NaCI). It has an apparent molecular mass of 32.4 kDa as determined by gel filtration chromatography, an apparent molecular mass of 33.5 kDa as determined by denaturing electrophoresis, and a pl of 10.1 and exhibits a single activity band after isoelectric focusing (IEF). It has a K<sub>m</sub>
of 0.0487 mg/mL and a V<sub>max</sub>
of 4.2378 nkat/mg of protein on 59% DE citrus pectin. Deblocking the N-terminus revealed a partial peptide composed of SVTPNV. Deesterification of non-calcium-sensitive pectin by 6.5% increased the calcium-sensitive pectin ratio (CSPR) from 0. 045 ± 0.011 to 0. 829 ± 0.033 but had little, if any, effect on pectin molecular weight. These properties indicate this enzyme will be useful for studying the PME mode of action as it relates to juice cloud destabilization.</EA>
<CC>002A35B09</CC>
<FD>Caractérisation; Séparation; Pectinesterase; Sensibilité résistance; Activité enzymatique; Citrus sinensis; Sel; Galacturonique acide; Pectine</FD>
<FG>Carboxylic ester hydrolases; Esterases; Hydrolases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Fruit; Polyoside; Biopolymère</FG>
<ED>Characterization; Separation; Pectinesterase; Sensitivity resistance; Enzymatic activity; Citrus sinensis; Salt; Galacturonic acid; Pectin</ED>
<EG>Carboxylic ester hydrolases; Esterases; Hydrolases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Fruit; Polysaccharide; Biopolymer</EG>
<SD>Caracterización; Separación; Pectinesterase; Sensibilidad resistencia; Actividad enzimática; Citrus sinensis; Sal; Galacturónico ácido; Pectina</SD>
<LO>INIST-7332.354000109555090500</LO>
<ID>03-0344968</ID>
</server>
</inist>
</record>
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