OrangerV1, PascalFrancis, Corpus, bibRecord, 000740

Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis

Identifieur interne : 000740 ( PascalFrancis/Corpus ); précédent : 000739; suivant : 000741

Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis

Auteurs : Masayuki Kita ; Tomoko Endo ; Takehiko Shimada ; Takaya Moriguchi ; Yutaka Hirata ; Shin Hasegawa ; Mitsuo Omura

Source :

Euphytica [ 0014-2336 ] ; 2003.

RBID : Pascal:03-0425093

Descripteurs français

Pascal (Inist)
- Saveur amère, Allèle, Marqueur génétique, Citrus unshiu, Citrus sinensis, Glucoside, Hexosyltransferases.

English descriptors

KwdEn :
- Allele, Bitter taste, Citrus sinensis, Citrus unshiu, Genetic marker, Glucoside, Hexosyltransferases.

Abstract

To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`01`	`1`		`@1 KITA (Masayuki)`
A11	`02`	`1`		`@1 ENDO (Tomoko)`
A11	`03`	`1`		`@1 SHIMADA (Takehiko)`
A11	`04`	`1`		`@1 MORIGUCHI (Takaya)`
A11	`05`	`1`		`@1 HIRATA (Yutaka)`
A11	`06`	`1`		`@1 HASEGAWA (Shin)`
A11	`07`	`1`		`@1 OMURA (Mitsuo)`
A14	`01`			`@1 Department of Citriculture, National Institute of Fruit Tree Science @2 Shimizuokitsu, Shizuoka 424-0292 @3 JPN @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 7 aut.`
A14	`02`			`@1 Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto @2 Tsukuba, Ibaraki 305-8605 @3 JPN @Z 1 aut. @Z 4 aut.`
A14	`03`			`@1 United Graduate School, Tokyo University of Agricultural and Technology @2 Fuchu, Tokyo 183-0054 @3 JPN @Z 5 aut.`
A14	`04`			`@1 Agricultural Research Service, Western Regional Research Center, USDA, 800 Buchanan Street @2 Albany, CA94710 @3 USA @Z 6 aut.`
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A44				`@0 0000 @1 © 2003 INIST-CNRS. All rights reserved.`
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C01	`01`		`ENG`	@0 To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.
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C03	`04`	`X`	`ENG`	`@0 Citrus unshiu @2 NS @5 10`
C03	`04`	`X`	`SPA`	`@0 Citrus unshiu @2 NS @5 10`
C03	`05`	`X`	`FRE`	`@0 Citrus sinensis @2 NS @5 11`
C03	`05`	`X`	`ENG`	`@0 Citrus sinensis @2 NS @5 11`
C03	`05`	`X`	`SPA`	`@0 Citrus sinensis @2 NS @5 11`
C03	`06`	`X`	`FRE`	`@0 Glucoside @5 15`
C03	`06`	`X`	`ENG`	`@0 Glucoside @5 15`
C03	`06`	`X`	`SPA`	`@0 Glucósido @5 15`
C03	`07`	`X`	`FRE`	`@0 Hexosyltransferases @2 FE @5 16`
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C07	`02`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`03`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
C07	`05`	`X`	`FRE`	`@0 Glycosyltransferases @2 FE`
C07	`05`	`X`	`ENG`	`@0 Glycosyltransferases @2 FE`
C07	`05`	`X`	`SPA`	`@0 Glycosyltransferases @2 FE`
C07	`06`	`X`	`FRE`	`@0 Transferases @2 FE`
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C07	`07`	`X`	`FRE`	`@0 Enzyme`
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C07	`08`	`X`	`SPA`	`@0 Agrios @5 39`
C07	`09`	`X`	`FRE`	`@0 Arbre fruitier @5 40`
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Format Inist (serveur)

NO :	PASCAL 03-0425093 INIST
ET :	Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis
AU :	KITA (Masayuki); ENDO (Tomoko); SHIMADA (Takehiko); MORIGUCHI (Takaya); HIRATA (Yutaka); HASEGAWA (Shin); OMURA (Mitsuo)
AF :	Department of Citriculture, National Institute of Fruit Tree Science/Shimizuokitsu, Shizuoka 424-0292/Japon (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto/Tsukuba, Ibaraki 305-8605/Japon (1 aut., 4 aut.); United Graduate School, Tokyo University of Agricultural and Technology/Fuchu, Tokyo 183-0054/Japon (5 aut.); Agricultural Research Service, Western Regional Research Center, USDA, 800 Buchanan Street/Albany, CA94710/Etats-Unis (6 aut.)
DT :	Publication en série; Niveau analytique
SO :	Euphytica; ISSN 0014-2336; Coden EUPHAA; Pays-Bas; Da. 2003; Vol. 132; No. 1; Pp. 87-94; Bibl. 17 ref.
LA :	Anglais
EA :	To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.
CC :	002A32D03D
FD :	Saveur amère; Allèle; Marqueur génétique; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases
FG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Agrume; Arbre fruitier
ED :	Bitter taste; Allele; Genetic marker; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases
EG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Citrus fruit; Fruit tree
SD :	Sabor amargo; Alelo; Marcador genético; Citrus unshiu; Citrus sinensis; Glucósido; Hexosyltransferases
LO :	INIST-8245.354000112530780100
ID :	03-0425093

Links to Exploration step

Pascal:03-0425093

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Allele</term>
<term>Bitter taste</term>
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<term>Genetic marker</term>
<term>Glucoside</term>
<term>Hexosyltransferases</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Saveur amère</term>
<term>Allèle</term>
<term>Marqueur génétique</term>
<term>Citrus unshiu</term>
<term>Citrus sinensis</term>
<term>Glucoside</term>
<term>Hexosyltransferases</term>
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<front><div type="abstract" xml:lang="en">To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.</div>
</front>
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<fA08 i1="01" i2="1" l="ENG"><s1>Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>KITA (Masayuki)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>ENDO (Tomoko)</s1>
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<fA11 i1="03" i2="1"><s1>SHIMADA (Takehiko)</s1>
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<fA11 i1="06" i2="1"><s1>HASEGAWA (Shin)</s1>
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<fA11 i1="07" i2="1"><s1>OMURA (Mitsuo)</s1>
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<fA14 i1="01"><s1>Department of Citriculture, National Institute of Fruit Tree Science</s1>
<s2>Shimizuokitsu, Shizuoka 424-0292</s2>
<s3>JPN</s3>
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<sZ>4 aut.</sZ>
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<fA14 i1="02"><s1>Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto</s1>
<s2>Tsukuba, Ibaraki 305-8605</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>United Graduate School, Tokyo University of Agricultural and Technology</s1>
<s2>Fuchu, Tokyo 183-0054</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Agricultural Research Service, Western Regional Research Center, USDA, 800 Buchanan Street</s1>
<s2>Albany, CA94710</s2>
<s3>USA</s3>
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<fC01 i1="01" l="ENG"><s0>To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.</s0>
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<s5>01</s5>
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<s5>01</s5>
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<s5>01</s5>
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<s5>03</s5>
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<s5>03</s5>
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<fC03 i1="04" i2="X" l="FRE"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
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<fC03 i1="04" i2="X" l="ENG"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
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<fC03 i1="05" i2="X" l="ENG"><s0>Citrus sinensis</s0>
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<s2>NS</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
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<s2>NS</s2>
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<fC07 i1="05" i2="X" l="FRE"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
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<fC07 i1="06" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
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<fC07 i1="07" i2="X" l="FRE"><s0>Enzyme</s0>
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<fC07 i1="07" i2="X" l="ENG"><s0>Enzyme</s0>
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<s5>40</s5>
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<server><NO>PASCAL 03-0425093 INIST</NO>
<ET>Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis</ET>
<AU>KITA (Masayuki); ENDO (Tomoko); SHIMADA (Takehiko); MORIGUCHI (Takaya); HIRATA (Yutaka); HASEGAWA (Shin); OMURA (Mitsuo)</AU>
<AF>Department of Citriculture, National Institute of Fruit Tree Science/Shimizuokitsu, Shizuoka 424-0292/Japon (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto/Tsukuba, Ibaraki 305-8605/Japon (1 aut., 4 aut.); United Graduate School, Tokyo University of Agricultural and Technology/Fuchu, Tokyo 183-0054/Japon (5 aut.); Agricultural Research Service, Western Regional Research Center, USDA, 800 Buchanan Street/Albany, CA94710/Etats-Unis (6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Euphytica; ISSN 0014-2336; Coden EUPHAA; Pays-Bas; Da. 2003; Vol. 132; No. 1; Pp. 87-94; Bibl. 17 ref.</SO>
<LA>Anglais</LA>
<EA>To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.</EA>
<CC>002A32D03D</CC>
<FD>Saveur amère; Allèle; Marqueur génétique; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Agrume; Arbre fruitier</FG>
<ED>Bitter taste; Allele; Genetic marker; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Citrus fruit; Fruit tree</EG>
<SD>Sabor amargo; Alelo; Marcador genético; Citrus unshiu; Citrus sinensis; Glucósido; Hexosyltransferases</SD>
<LO>INIST-8245.354000112530780100</LO>
<ID>03-0425093</ID>
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Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis

Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis

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