Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis
Identifieur interne : 000740 ( PascalFrancis/Corpus ); précédent : 000739; suivant : 000741Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis
Auteurs : Masayuki Kita ; Tomoko Endo ; Takehiko Shimada ; Takaya Moriguchi ; Yutaka Hirata ; Shin Hasegawa ; Mitsuo OmuraSource :
- Euphytica [ 0014-2336 ] ; 2003.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.
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NO : | PASCAL 03-0425093 INIST |
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ET : | Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis |
AU : | KITA (Masayuki); ENDO (Tomoko); SHIMADA (Takehiko); MORIGUCHI (Takaya); HIRATA (Yutaka); HASEGAWA (Shin); OMURA (Mitsuo) |
AF : | Department of Citriculture, National Institute of Fruit Tree Science/Shimizuokitsu, Shizuoka 424-0292/Japon (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto/Tsukuba, Ibaraki 305-8605/Japon (1 aut., 4 aut.); United Graduate School, Tokyo University of Agricultural and Technology/Fuchu, Tokyo 183-0054/Japon (5 aut.); Agricultural Research Service, Western Regional Research Center, USDA, 800 Buchanan Street/Albany, CA94710/Etats-Unis (6 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Euphytica; ISSN 0014-2336; Coden EUPHAA; Pays-Bas; Da. 2003; Vol. 132; No. 1; Pp. 87-94; Bibl. 17 ref. |
LA : | Anglais |
EA : | To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits. |
CC : | 002A32D03D |
FD : | Saveur amère; Allèle; Marqueur génétique; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases |
FG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Agrume; Arbre fruitier |
ED : | Bitter taste; Allele; Genetic marker; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases |
EG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Citrus fruit; Fruit tree |
SD : | Sabor amargo; Alelo; Marcador genético; Citrus unshiu; Citrus sinensis; Glucósido; Hexosyltransferases |
LO : | INIST-8245.354000112530780100 |
ID : | 03-0425093 |
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Pascal:03-0425093Le document en format XML
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<front><div type="abstract" xml:lang="en">To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.</div>
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<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Marcador genético</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Glucoside</s0>
<s5>15</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Glucoside</s0>
<s5>15</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Glucósido</s0>
<s5>15</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Hexosyltransferases</s0>
<s2>FE</s2>
<s5>16</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Hexosyltransferases</s0>
<s2>FE</s2>
<s5>16</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Hexosyltransferases</s0>
<s2>FE</s2>
<s5>16</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Glycosyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Enzyme</s0>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Enzyme</s0>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Enzima</s0>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Arbre fruitier</s0>
<s5>40</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Fruit tree</s0>
<s5>40</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Arbol frutal</s0>
<s5>40</s5>
</fC07>
<fN21><s1>293</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 03-0425093 INIST</NO>
<ET>Allelic structures of UDP-glucose:limonoid glucosyltransferase affect limonoid bitterness in Citrus unshiu and C. sinensis</ET>
<AU>KITA (Masayuki); ENDO (Tomoko); SHIMADA (Takehiko); MORIGUCHI (Takaya); HIRATA (Yutaka); HASEGAWA (Shin); OMURA (Mitsuo)</AU>
<AF>Department of Citriculture, National Institute of Fruit Tree Science/Shimizuokitsu, Shizuoka 424-0292/Japon (1 aut., 2 aut., 3 aut., 4 aut., 7 aut.); Department of Plant, Cell and Environment, National Institute of Fruit Tree Science, Fujimoto/Tsukuba, Ibaraki 305-8605/Japon (1 aut., 4 aut.); United Graduate School, Tokyo University of Agricultural and Technology/Fuchu, Tokyo 183-0054/Japon (5 aut.); Agricultural Research Service, Western Regional Research Center, USDA, 800 Buchanan Street/Albany, CA94710/Etats-Unis (6 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Euphytica; ISSN 0014-2336; Coden EUPHAA; Pays-Bas; Da. 2003; Vol. 132; No. 1; Pp. 87-94; Bibl. 17 ref.</SO>
<LA>Anglais</LA>
<EA>To develop a molecular indicator for determining the level of limonoid bitterness in Satsuma mandarin (Citrus unshiu) and navel orange (C. sinensis), the genetic background of the transcription of the limonoid glucosyltransferase gene was characterized in relation to the accumulation of non-bitter limonoid glucosides. Two types of cDNA clones (CitLGT-1 and CitLGT-2) encoding limonoid glucosyltransferase were isolated and characterized. The relationships between the gene expression patterns of CitLGTs and the non-bitter limonoid glucoside accumulation as well as the allelic structures of CitLGTs were determined. Southern blot analysis and segregation analysis of the progenies between Satsuma mandarin and tangor (C. unshiu x C. sinensis) indicated that CitLGT-1 and CitLGT-2 are transcribed from the alleles in a single locus. The genotype of the CitLGT locus in navel orange is homozygous for CitLGT-1 (CitLGT-1/CitLGT-1), whereas that in Satsuma mandarin is heterozygous for CitLGT-1 and CitLGT-2 (CitLGT-1/CitLGT-2). The levels of non-bitter limonoid glucosides in navel orange fruit were quite low at the early-to mid-developmental stage due to a defective CitLGT-2, whereas both non-bitter limonoid glucoside content and CitLGT-2 expression in Satsuma mandarin fruit were high throughout fruit development. These results indicate that the expression of CitLGT-2 is a prerequisite for the accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stage of fruit. The allelism of the two CitLGTs, that is, whether the CitLGT-2 is present or not, is a useful molecular indicator for predetermining the levels of accumulation of non-bitter limonoid glucosides at the early- to mid-developmental stages of Satsuma mandarin and navel orange fruits.</EA>
<CC>002A32D03D</CC>
<FD>Saveur amère; Allèle; Marqueur génétique; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Agrume; Arbre fruitier</FG>
<ED>Bitter taste; Allele; Genetic marker; Citrus unshiu; Citrus sinensis; Glucoside; Hexosyltransferases</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Glycosyltransferases; Transferases; Enzyme; Citrus fruit; Fruit tree</EG>
<SD>Sabor amargo; Alelo; Marcador genético; Citrus unshiu; Citrus sinensis; Glucósido; Hexosyltransferases</SD>
<LO>INIST-8245.354000112530780100</LO>
<ID>03-0425093</ID>
</server>
</inist>
</record>
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