OrangerV1, PascalFrancis, Corpus, bibRecord, 000705

Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit

Identifieur interne : 000705 ( PascalFrancis/Corpus ); précédent : 000704; suivant : 000706

Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit

Auteurs : Masaya Kato ; Yoshinori Ikoma ; Hikaru Matsumoto ; Minoru Sugiura ; Hiroshi Hyodo ; Masamichi Yano

Source :

Plant physiology : (Bethesda) [ 0032-0889 ] ; 2004.

RBID : Pascal:04-0150864

Descripteurs français

Pascal (Inist)
- Accumulation biologique, Expression génique, Voie métabolique, Biosynthèse, Maturation, Fruit, Régulation, RNA messager, Carotene 7,8-desaturase, Citrus unshiu, Citrus limon, Citrus sinensis, Carotène, Xanthophylle, Phytoene synthase, Phytoene desaturase, Carotenoide isomerase, Lycopene β cylase, Phytoène, β-Ring hydroxylase, Zeaxanthine epoxidase, Lycopene ε cyclase.

English descriptors

KwdEn :
- Biological accumulation, Biosynthesis, Carotene, Carotene 7,8-desaturase, Citrus limon, Citrus sinensis, Citrus unshiu, Fruit, Gene expression, Messenger RNA, Metabolic pathway, Regulation(control), Ripening, Xanthophyll.

Abstract

The relationship between carotenoid accumulation and the expression of carotenoid biosynthetic genes during fruit maturation was investigated in three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). We cloned the cDNAs for phytoene synthase (CitPSY), phytoene desaturase (CitPDS), ζ-carotene (car) desaturase (CitZDS), carotenoid isomerase (CitCRTISO), lycopene β-cyclase (CitLCYb), β-ring hydroxylase (CitHYb), zeaxanthin (zea) epoxidase (CitZEP), and lycopene ∈-cyclase (CitLCYe) from Satsuma mandarin, which shared high identities in nucleotide sequences with Valencia orange, Lisbon lemon, and other plant species. With the transition of peel color from green to orange, the change from β,∈-carotenoid (a-car and lutein) accumulation to β,β-carotenoid (β-car, β-cryptoxanthin, zea, and violaxanthin) accumulation was observed in the flavedos of Satsuma mandarin and Valencia orange, accompanying the disappearance of CitLCYe transcripts and the increase in CitLCYb transcripts. Even in green fruit, high levels of β,∈-carotenoids and CitLCYe transcripts were not observed in the juice sacs. As fruit maturation progressed in Satsuma mandarin and Valencia orange, a simultaneous increase in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb, CitHYb, and CitZEP) led to massive β,β-xanthophyll (β-cryptoxanthin, zea, and violaxanthin) accumulation in both the flavedo and juice sacs. The gene expression of CitCRTISO was kept low or decreased in the flavedo during massive β,β-xanthophyll accumulation. In the flavedo of Lisbon lemon and Satsuma mandarin, massive accumulation of phytoene was observed with a decrease in the transcript level for CitPDS. Thus, the carotenoid accumulation during citrus fruit maturation was highly regulated by the coordination of the expression among carotenoid biosynthetic genes. In this paper, the mechanism leading to diversity in β,β-xanthophyll compositions between Satsuma mandarin and Valencia orange was also discussed on the basis of the substrate specificity of β-ring hydroxylase and the balance of expression between upstream synthesis genes (CitPSY, CitPDS, CitZDS, and CitLCYb) and downstream synthesis genes (CitHYb and CitZEP).

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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Format Inist (serveur)

NO :	PASCAL 04-0150864 INIST
ET :	Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit
AU :	KATO (Masaya); IKOMA (Yoshinori); MATSUMOTO (Hikaru); SUGIURA (Minoru); HYODO (Hiroshi); YANO (Masamichi)
AF :	Department of Citrus Research, National Institute of Fruit Tree Science/Shimizu-okitsunakacho, Shizuoka 424-0292/Japon (1 aut., 2 aut., 3 aut., 4 aut., 6 aut.); Department of Biological Sciences, Faculty of Agriculture, Shizuoka University/836 Ohya, Shizuoka 422-8529/Japon (5 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant physiology : (Bethesda); ISSN 0032-0889; Coden PPHYA5; Etats-Unis; Da. 2004; Vol. 134; No. 2; Pp. 824-837; Bibl. 34 ref.
LA :	Anglais
EA :	The relationship between carotenoid accumulation and the expression of carotenoid biosynthetic genes during fruit maturation was investigated in three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). We cloned the cDNAs for phytoene synthase (CitPSY), phytoene desaturase (CitPDS), ζ-carotene (car) desaturase (CitZDS), carotenoid isomerase (CitCRTISO), lycopene β-cyclase (CitLCYb), β-ring hydroxylase (CitHYb), zeaxanthin (zea) epoxidase (CitZEP), and lycopene ∈-cyclase (CitLCYe) from Satsuma mandarin, which shared high identities in nucleotide sequences with Valencia orange, Lisbon lemon, and other plant species. With the transition of peel color from green to orange, the change from β,∈-carotenoid (a-car and lutein) accumulation to β,β-carotenoid (β-car, β-cryptoxanthin, zea, and violaxanthin) accumulation was observed in the flavedos of Satsuma mandarin and Valencia orange, accompanying the disappearance of CitLCYe transcripts and the increase in CitLCYb transcripts. Even in green fruit, high levels of β,∈-carotenoids and CitLCYe transcripts were not observed in the juice sacs. As fruit maturation progressed in Satsuma mandarin and Valencia orange, a simultaneous increase in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb, CitHYb, and CitZEP) led to massive β,β-xanthophyll (β-cryptoxanthin, zea, and violaxanthin) accumulation in both the flavedo and juice sacs. The gene expression of CitCRTISO was kept low or decreased in the flavedo during massive β,β-xanthophyll accumulation. In the flavedo of Lisbon lemon and Satsuma mandarin, massive accumulation of phytoene was observed with a decrease in the transcript level for CitPDS. Thus, the carotenoid accumulation during citrus fruit maturation was highly regulated by the coordination of the expression among carotenoid biosynthetic genes. In this paper, the mechanism leading to diversity in β,β-xanthophyll compositions between Satsuma mandarin and Valencia orange was also discussed on the basis of the substrate specificity of β-ring hydroxylase and the balance of expression between upstream synthesis genes (CitPSY, CitPDS, CitZDS, and CitLCYb) and downstream synthesis genes (CitHYb and CitZEP).
CC :	002A32E06E; 002A10F05
FD :	Accumulation biologique; Expression génique; Voie métabolique; Biosynthèse; Maturation; Fruit; Régulation; RNA messager; Carotene 7,8-desaturase; Citrus unshiu; Citrus limon; Citrus sinensis; Carotène; Xanthophylle; Phytoene synthase; Phytoene desaturase; Carotenoide isomerase; Lycopene β cylase; Phytoène; β-Ring hydroxylase; Zeaxanthine epoxidase; Lycopene ε cyclase
FG :	Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Caroténoïde; Pigment organique
ED :	Biological accumulation; Gene expression; Metabolic pathway; Biosynthesis; Ripening; Fruit; Regulation(control); Messenger RNA; Carotene 7,8-desaturase; Citrus unshiu; Citrus limon; Citrus sinensis; Carotene; Xanthophyll
EG :	Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Carotenoid; Organic pigment
SD :	Acumulación biológica; Expresión genética; Vía métabolica; Biosíntesis; Maduración; Fruto; Regulación; RNA mensajero; Carotene 7,8-desaturase; Citrus unshiu; Citrus limon; Citrus sinensis; Caroteno; Xantófilo
LO :	INIST-3000.354000119223910270
ID :	04-0150864

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Le document en format XML

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<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hyodo, Hiroshi" sort="Hyodo, Hiroshi" uniqKey="Hyodo H" first="Hiroshi" last="Hyodo">Hiroshi Hyodo</name>
<affiliation><inist:fA14 i1="02"><s1>Department of Biological Sciences, Faculty of Agriculture, Shizuoka University</s1>
<s2>836 Ohya, Shizuoka 422-8529</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Yano, Masamichi" sort="Yano, Masamichi" uniqKey="Yano M" first="Masamichi" last="Yano">Masamichi Yano</name>
<affiliation><inist:fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science</s1>
<s2>Shimizu-okitsunakacho, Shizuoka 424-0292</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant physiology : (Bethesda)</title>
<title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title>
<idno type="ISSN">0032-0889</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant physiology : (Bethesda)</title>
<title level="j" type="abbreviated">Plant physiol. : (Bethesda)</title>
<idno type="ISSN">0032-0889</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological accumulation</term>
<term>Biosynthesis</term>
<term>Carotene</term>
<term>Carotene 7,8-desaturase</term>
<term>Citrus limon</term>
<term>Citrus sinensis</term>
<term>Citrus unshiu</term>
<term>Fruit</term>
<term>Gene expression</term>
<term>Messenger RNA</term>
<term>Metabolic pathway</term>
<term>Regulation(control)</term>
<term>Ripening</term>
<term>Xanthophyll</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Accumulation biologique</term>
<term>Expression génique</term>
<term>Voie métabolique</term>
<term>Biosynthèse</term>
<term>Maturation</term>
<term>Fruit</term>
<term>Régulation</term>
<term>RNA messager</term>
<term>Carotene 7,8-desaturase</term>
<term>Citrus unshiu</term>
<term>Citrus limon</term>
<term>Citrus sinensis</term>
<term>Carotène</term>
<term>Xanthophylle</term>
<term>Phytoene synthase</term>
<term>Phytoene desaturase</term>
<term>Carotenoide isomerase</term>
<term>Lycopene β cylase</term>
<term>Phytoène</term>
<term>β-Ring hydroxylase</term>
<term>Zeaxanthine epoxidase</term>
<term>Lycopene ε cyclase</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The relationship between carotenoid accumulation and the expression of carotenoid biosynthetic genes during fruit maturation was investigated in three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). We cloned the cDNAs for phytoene synthase (CitPSY), phytoene desaturase (CitPDS), ζ-carotene (car) desaturase (CitZDS), carotenoid isomerase (CitCRTISO), lycopene β-cyclase (CitLCYb), β-ring hydroxylase (CitHYb), zeaxanthin (zea) epoxidase (CitZEP), and lycopene ∈-cyclase (CitLCYe) from Satsuma mandarin, which shared high identities in nucleotide sequences with Valencia orange, Lisbon lemon, and other plant species. With the transition of peel color from green to orange, the change from β,∈-carotenoid (a-car and lutein) accumulation to β,β-carotenoid (β-car, β-cryptoxanthin, zea, and violaxanthin) accumulation was observed in the flavedos of Satsuma mandarin and Valencia orange, accompanying the disappearance of CitLCYe transcripts and the increase in CitLCYb transcripts. Even in green fruit, high levels of β,∈-carotenoids and CitLCYe transcripts were not observed in the juice sacs. As fruit maturation progressed in Satsuma mandarin and Valencia orange, a simultaneous increase in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb, CitHYb, and CitZEP) led to massive β,β-xanthophyll (β-cryptoxanthin, zea, and violaxanthin) accumulation in both the flavedo and juice sacs. The gene expression of CitCRTISO was kept low or decreased in the flavedo during massive β,β-xanthophyll accumulation. In the flavedo of Lisbon lemon and Satsuma mandarin, massive accumulation of phytoene was observed with a decrease in the transcript level for CitPDS. Thus, the carotenoid accumulation during citrus fruit maturation was highly regulated by the coordination of the expression among carotenoid biosynthetic genes. In this paper, the mechanism leading to diversity in β,β-xanthophyll compositions between Satsuma mandarin and Valencia orange was also discussed on the basis of the substrate specificity of β-ring hydroxylase and the balance of expression between upstream synthesis genes (CitPSY, CitPDS, CitZDS, and CitLCYb) and downstream synthesis genes (CitHYb and CitZEP).</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0032-0889</s0>
</fA01>
<fA02 i1="01"><s0>PPHYA5</s0>
</fA02>
<fA03 i2="1"><s0>Plant physiol. : (Bethesda)</s0>
</fA03>
<fA05><s2>134</s2>
</fA05>
<fA06><s2>2</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>KATO (Masaya)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>IKOMA (Yoshinori)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>MATSUMOTO (Hikaru)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>SUGIURA (Minoru)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>HYODO (Hiroshi)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>YANO (Masamichi)</s1>
</fA11>
<fA14 i1="01"><s1>Department of Citrus Research, National Institute of Fruit Tree Science</s1>
<s2>Shimizu-okitsunakacho, Shizuoka 424-0292</s2>
<s3>JPN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Department of Biological Sciences, Faculty of Agriculture, Shizuoka University</s1>
<s2>836 Ohya, Shizuoka 422-8529</s2>
<s3>JPN</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA20><s1>824-837</s1>
</fA20>
<fA21><s1>2004</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>3000</s2>
<s5>354000119223910270</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2004 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>34 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>04-0150864</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Plant physiology : (Bethesda)</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The relationship between carotenoid accumulation and the expression of carotenoid biosynthetic genes during fruit maturation was investigated in three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). We cloned the cDNAs for phytoene synthase (CitPSY), phytoene desaturase (CitPDS), ζ-carotene (car) desaturase (CitZDS), carotenoid isomerase (CitCRTISO), lycopene β-cyclase (CitLCYb), β-ring hydroxylase (CitHYb), zeaxanthin (zea) epoxidase (CitZEP), and lycopene ∈-cyclase (CitLCYe) from Satsuma mandarin, which shared high identities in nucleotide sequences with Valencia orange, Lisbon lemon, and other plant species. With the transition of peel color from green to orange, the change from β,∈-carotenoid (a-car and lutein) accumulation to β,β-carotenoid (β-car, β-cryptoxanthin, zea, and violaxanthin) accumulation was observed in the flavedos of Satsuma mandarin and Valencia orange, accompanying the disappearance of CitLCYe transcripts and the increase in CitLCYb transcripts. Even in green fruit, high levels of β,∈-carotenoids and CitLCYe transcripts were not observed in the juice sacs. As fruit maturation progressed in Satsuma mandarin and Valencia orange, a simultaneous increase in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb, CitHYb, and CitZEP) led to massive β,β-xanthophyll (β-cryptoxanthin, zea, and violaxanthin) accumulation in both the flavedo and juice sacs. The gene expression of CitCRTISO was kept low or decreased in the flavedo during massive β,β-xanthophyll accumulation. In the flavedo of Lisbon lemon and Satsuma mandarin, massive accumulation of phytoene was observed with a decrease in the transcript level for CitPDS. Thus, the carotenoid accumulation during citrus fruit maturation was highly regulated by the coordination of the expression among carotenoid biosynthetic genes. In this paper, the mechanism leading to diversity in β,β-xanthophyll compositions between Satsuma mandarin and Valencia orange was also discussed on the basis of the substrate specificity of β-ring hydroxylase and the balance of expression between upstream synthesis genes (CitPSY, CitPDS, CitZDS, and CitLCYb) and downstream synthesis genes (CitHYb and CitZEP).</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A32E06E</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A10F05</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Accumulation biologique</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Biological accumulation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Acumulación biológica</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Expression génique</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Gene expression</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Expresión genética</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Voie métabolique</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Metabolic pathway</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Vía métabolica</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Biosynthèse</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Biosynthesis</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Biosíntesis</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Maturation</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Ripening</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Maduración</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Fruit</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Fruit</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Fruto</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Régulation</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Regulation(control)</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Regulación</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>RNA messager</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Messenger RNA</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>RNA mensajero</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Carotene 7,8-desaturase</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Carotene 7,8-desaturase</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Carotene 7,8-desaturase</s0>
<s2>FE</s2>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Citrus unshiu</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Citrus limon</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Citrus limon</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Citrus limon</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Carotène</s0>
<s5>15</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Carotene</s0>
<s5>15</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Caroteno</s0>
<s5>15</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Xanthophylle</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Xanthophyll</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Xantófilo</s0>
<s2>NK</s2>
<s5>16</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Phytoene synthase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>68</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Phytoene desaturase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>69</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Carotenoide isomerase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>70</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Lycopene β cylase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>71</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>Phytoène</s0>
<s2>NK</s2>
<s4>INC</s4>
<s5>81</s5>
</fC03>
<fC03 i1="20" i2="X" l="FRE"><s0>β-Ring hydroxylase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>90</s5>
</fC03>
<fC03 i1="21" i2="X" l="FRE"><s0>Zeaxanthine epoxidase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>91</s5>
</fC03>
<fC03 i1="22" i2="X" l="FRE"><s0>Lycopene ε cyclase</s0>
<s2>FE</s2>
<s4>INC</s4>
<s5>92</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Oxidoreductases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Caroténoïde</s0>
<s5>50</s5>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Carotenoid</s0>
<s5>50</s5>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Carotenoide</s0>
<s5>50</s5>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Pigment organique</s0>
<s5>51</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Organic pigment</s0>
<s5>51</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Pigmento orgánico</s0>
<s5>51</s5>
</fC07>
<fN21><s1>096</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 04-0150864 INIST</NO>
<ET>Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit</ET>
<AU>KATO (Masaya); IKOMA (Yoshinori); MATSUMOTO (Hikaru); SUGIURA (Minoru); HYODO (Hiroshi); YANO (Masamichi)</AU>
<AF>Department of Citrus Research, National Institute of Fruit Tree Science/Shimizu-okitsunakacho, Shizuoka 424-0292/Japon (1 aut., 2 aut., 3 aut., 4 aut., 6 aut.); Department of Biological Sciences, Faculty of Agriculture, Shizuoka University/836 Ohya, Shizuoka 422-8529/Japon (5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant physiology : (Bethesda); ISSN 0032-0889; Coden PPHYA5; Etats-Unis; Da. 2004; Vol. 134; No. 2; Pp. 824-837; Bibl. 34 ref.</SO>
<LA>Anglais</LA>
<EA>The relationship between carotenoid accumulation and the expression of carotenoid biosynthetic genes during fruit maturation was investigated in three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). We cloned the cDNAs for phytoene synthase (CitPSY), phytoene desaturase (CitPDS), ζ-carotene (car) desaturase (CitZDS), carotenoid isomerase (CitCRTISO), lycopene β-cyclase (CitLCYb), β-ring hydroxylase (CitHYb), zeaxanthin (zea) epoxidase (CitZEP), and lycopene ∈-cyclase (CitLCYe) from Satsuma mandarin, which shared high identities in nucleotide sequences with Valencia orange, Lisbon lemon, and other plant species. With the transition of peel color from green to orange, the change from β,∈-carotenoid (a-car and lutein) accumulation to β,β-carotenoid (β-car, β-cryptoxanthin, zea, and violaxanthin) accumulation was observed in the flavedos of Satsuma mandarin and Valencia orange, accompanying the disappearance of CitLCYe transcripts and the increase in CitLCYb transcripts. Even in green fruit, high levels of β,∈-carotenoids and CitLCYe transcripts were not observed in the juice sacs. As fruit maturation progressed in Satsuma mandarin and Valencia orange, a simultaneous increase in the expression of genes (CitPSY, CitPDS, CitZDS, CitLCYb, CitHYb, and CitZEP) led to massive β,β-xanthophyll (β-cryptoxanthin, zea, and violaxanthin) accumulation in both the flavedo and juice sacs. The gene expression of CitCRTISO was kept low or decreased in the flavedo during massive β,β-xanthophyll accumulation. In the flavedo of Lisbon lemon and Satsuma mandarin, massive accumulation of phytoene was observed with a decrease in the transcript level for CitPDS. Thus, the carotenoid accumulation during citrus fruit maturation was highly regulated by the coordination of the expression among carotenoid biosynthetic genes. In this paper, the mechanism leading to diversity in β,β-xanthophyll compositions between Satsuma mandarin and Valencia orange was also discussed on the basis of the substrate specificity of β-ring hydroxylase and the balance of expression between upstream synthesis genes (CitPSY, CitPDS, CitZDS, and CitLCYb) and downstream synthesis genes (CitHYb and CitZEP).</EA>
<CC>002A32E06E; 002A10F05</CC>
<FD>Accumulation biologique; Expression génique; Voie métabolique; Biosynthèse; Maturation; Fruit; Régulation; RNA messager; Carotene 7,8-desaturase; Citrus unshiu; Citrus limon; Citrus sinensis; Carotène; Xanthophylle; Phytoene synthase; Phytoene desaturase; Carotenoide isomerase; Lycopene β cylase; Phytoène; β-Ring hydroxylase; Zeaxanthine epoxidase; Lycopene ε cyclase</FD>
<FG>Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Caroténoïde; Pigment organique</FG>
<ED>Biological accumulation; Gene expression; Metabolic pathway; Biosynthesis; Ripening; Fruit; Regulation(control); Messenger RNA; Carotene 7,8-desaturase; Citrus unshiu; Citrus limon; Citrus sinensis; Carotene; Xanthophyll</ED>
<EG>Oxidoreductases; Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Carotenoid; Organic pigment</EG>
<SD>Acumulación biológica; Expresión genética; Vía métabolica; Biosíntesis; Maduración; Fruto; Regulación; RNA mensajero; Carotene 7,8-desaturase; Citrus unshiu; Citrus limon; Citrus sinensis; Caroteno; Xantófilo</SD>
<LO>INIST-3000.354000119223910270</LO>
<ID>04-0150864</ID>
</server>
</inist>
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Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit

Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit

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