An experimental inoculation system to study citrus-Xylella fastidiosa interactions
Identifieur interne : 000628 ( PascalFrancis/Corpus ); précédent : 000627; suivant : 000629An experimental inoculation system to study citrus-Xylella fastidiosa interactions
Auteurs : S. A. Lopes ; D. C. Teixeira ; N. G. Fernandes ; A. J. Ayres ; S. C. Z. Torres ; J. C. Barbosa ; I. W. B. LiSource :
- Plant disease [ 0191-2917 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.
Notice en format standard (ISO 2709)
Pour connaître la documentation sur le format Inist Standard.
pA |
|
---|
Format Inist (serveur)
NO : | PASCAL 05-0185753 INIST |
---|---|
ET : | An experimental inoculation system to study citrus-Xylella fastidiosa interactions |
AU : | LOPES (S. A.); TEIXEIRA (D. C.); FERNANDES (N. G.); AYRES (A. J.); TORRES (S. C. Z.); BARBOSA (J. C.); LI (I. W. B.) |
AF : | FUNDECITRUS/Araraquara, SP, 14801-970/Brésil (1 aut., 2 aut., 3 aut., 4 aut.); UNAERP,/Ribeirao Preto, SP, 14096-380/Brésil (5 aut.); UNESP/Jaboticabal, SP, 14884-900/Brésil (6 aut.); USDA-ARS/Beltsville, MD 20705-2350/Etats-Unis (7 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Plant disease; ISSN 0191-2917; Coden PLDIDE; Etats-Unis; Da. 2005; Vol. 89; No. 3; Pp. 250-254; Bibl. 16 ref. |
LA : | Anglais |
EA : | Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions. |
CC : | 002A34 |
FD : | Citrus; Etude expérimentale; Microbiologie; Phytopathologie; Phytopathogène; Plante; Xylella fastidiosa |
FG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume |
ED : | Citrus; Experimental study; Microbiology; Plant pathology; Plant pathogen; Plant |
EG : | Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit |
SD : | Citrus; Estudio experimental; Microbiología; Fitopatología; Fitopatógeno; Planta |
LO : | INIST-12673.354000125080580060 |
ID : | 05-0185753 |
Links to Exploration step
Pascal:05-0185753Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">An experimental inoculation system to study citrus-Xylella fastidiosa interactions</title>
<author><name sortKey="Lopes, S A" sort="Lopes, S A" uniqKey="Lopes S" first="S. A." last="Lopes">S. A. Lopes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Teixeira, D C" sort="Teixeira, D C" uniqKey="Teixeira D" first="D. C." last="Teixeira">D. C. Teixeira</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Fernandes, N G" sort="Fernandes, N G" uniqKey="Fernandes N" first="N. G." last="Fernandes">N. G. Fernandes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ayres, A J" sort="Ayres, A J" uniqKey="Ayres A" first="A. J." last="Ayres">A. J. Ayres</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Torres, S C Z" sort="Torres, S C Z" uniqKey="Torres S" first="S. C. Z." last="Torres">S. C. Z. Torres</name>
<affiliation><inist:fA14 i1="02"><s1>UNAERP,</s1>
<s2>Ribeirao Preto, SP, 14096-380</s2>
<s3>BRA</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Barbosa, J C" sort="Barbosa, J C" uniqKey="Barbosa J" first="J. C." last="Barbosa">J. C. Barbosa</name>
<affiliation><inist:fA14 i1="03"><s1>UNESP</s1>
<s2>Jaboticabal, SP, 14884-900</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Li, I W B" sort="Li, I W B" uniqKey="Li I" first="I. W. B." last="Li">I. W. B. Li</name>
<affiliation><inist:fA14 i1="04"><s1>USDA-ARS</s1>
<s2>Beltsville, MD 20705-2350</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">05-0185753</idno>
<date when="2005">2005</date>
<idno type="stanalyst">PASCAL 05-0185753 INIST</idno>
<idno type="RBID">Pascal:05-0185753</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000628</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">An experimental inoculation system to study citrus-Xylella fastidiosa interactions</title>
<author><name sortKey="Lopes, S A" sort="Lopes, S A" uniqKey="Lopes S" first="S. A." last="Lopes">S. A. Lopes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Teixeira, D C" sort="Teixeira, D C" uniqKey="Teixeira D" first="D. C." last="Teixeira">D. C. Teixeira</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Fernandes, N G" sort="Fernandes, N G" uniqKey="Fernandes N" first="N. G." last="Fernandes">N. G. Fernandes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ayres, A J" sort="Ayres, A J" uniqKey="Ayres A" first="A. J." last="Ayres">A. J. Ayres</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Torres, S C Z" sort="Torres, S C Z" uniqKey="Torres S" first="S. C. Z." last="Torres">S. C. Z. Torres</name>
<affiliation><inist:fA14 i1="02"><s1>UNAERP,</s1>
<s2>Ribeirao Preto, SP, 14096-380</s2>
<s3>BRA</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Barbosa, J C" sort="Barbosa, J C" uniqKey="Barbosa J" first="J. C." last="Barbosa">J. C. Barbosa</name>
<affiliation><inist:fA14 i1="03"><s1>UNESP</s1>
<s2>Jaboticabal, SP, 14884-900</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Li, I W B" sort="Li, I W B" uniqKey="Li I" first="I. W. B." last="Li">I. W. B. Li</name>
<affiliation><inist:fA14 i1="04"><s1>USDA-ARS</s1>
<s2>Beltsville, MD 20705-2350</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant disease</title>
<title level="j" type="abbreviated">Plant dis.</title>
<idno type="ISSN">0191-2917</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant disease</title>
<title level="j" type="abbreviated">Plant dis.</title>
<idno type="ISSN">0191-2917</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus</term>
<term>Experimental study</term>
<term>Microbiology</term>
<term>Plant</term>
<term>Plant pathogen</term>
<term>Plant pathology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Citrus</term>
<term>Etude expérimentale</term>
<term>Microbiologie</term>
<term>Phytopathologie</term>
<term>Phytopathogène</term>
<term>Plante</term>
<term>Xylella fastidiosa</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0191-2917</s0>
</fA01>
<fA02 i1="01"><s0>PLDIDE</s0>
</fA02>
<fA03 i2="1"><s0>Plant dis.</s0>
</fA03>
<fA05><s2>89</s2>
</fA05>
<fA06><s2>3</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>An experimental inoculation system to study citrus-Xylella fastidiosa interactions</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LOPES (S. A.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>TEIXEIRA (D. C.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>FERNANDES (N. G.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>AYRES (A. J.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>TORRES (S. C. Z.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BARBOSA (J. C.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>LI (I. W. B.)</s1>
</fA11>
<fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>UNAERP,</s1>
<s2>Ribeirao Preto, SP, 14096-380</s2>
<s3>BRA</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>UNESP</s1>
<s2>Jaboticabal, SP, 14884-900</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>USDA-ARS</s1>
<s2>Beltsville, MD 20705-2350</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>250-254</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>12673</s2>
<s5>354000125080580060</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>16 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0185753</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Plant disease</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A34</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Citrus</s0>
<s2>NS</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Citrus</s0>
<s2>NS</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Citrus</s0>
<s2>NS</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Etude expérimentale</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Experimental study</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Estudio experimental</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Phytopathologie</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Plant pathology</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Fitopatología</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Phytopathogène</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Plant pathogen</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Fitopatógeno</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Plante</s0>
<s5>45</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Plant</s0>
<s5>45</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Planta</s0>
<s5>45</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Xylella fastidiosa</s0>
<s4>INC</s4>
<s5>79</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Agrume</s0>
<s5>17</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>17</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Agrios</s0>
<s5>17</s5>
</fC07>
<fN21><s1>122</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 05-0185753 INIST</NO>
<ET>An experimental inoculation system to study citrus-Xylella fastidiosa interactions</ET>
<AU>LOPES (S. A.); TEIXEIRA (D. C.); FERNANDES (N. G.); AYRES (A. J.); TORRES (S. C. Z.); BARBOSA (J. C.); LI (I. W. B.)</AU>
<AF>FUNDECITRUS/Araraquara, SP, 14801-970/Brésil (1 aut., 2 aut., 3 aut., 4 aut.); UNAERP,/Ribeirao Preto, SP, 14096-380/Brésil (5 aut.); UNESP/Jaboticabal, SP, 14884-900/Brésil (6 aut.); USDA-ARS/Beltsville, MD 20705-2350/Etats-Unis (7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant disease; ISSN 0191-2917; Coden PLDIDE; Etats-Unis; Da. 2005; Vol. 89; No. 3; Pp. 250-254; Bibl. 16 ref.</SO>
<LA>Anglais</LA>
<EA>Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.</EA>
<CC>002A34</CC>
<FD>Citrus; Etude expérimentale; Microbiologie; Phytopathologie; Phytopathogène; Plante; Xylella fastidiosa</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume</FG>
<ED>Citrus; Experimental study; Microbiology; Plant pathology; Plant pathogen; Plant</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit</EG>
<SD>Citrus; Estudio experimental; Microbiología; Fitopatología; Fitopatógeno; Planta</SD>
<LO>INIST-12673.354000125080580060</LO>
<ID>05-0185753</ID>
</server>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PascalFrancis/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000628 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000628 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= PascalFrancis |étape= Corpus |type= RBID |clé= Pascal:05-0185753 |texte= An experimental inoculation system to study citrus-Xylella fastidiosa interactions }}
![]() | This area was generated with Dilib version V0.6.25. | ![]() |