OrangerV1, PascalFrancis, Corpus, bibRecord, 000628

An experimental inoculation system to study citrus-Xylella fastidiosa interactions

Identifieur interne : 000628 ( PascalFrancis/Corpus ); précédent : 000627; suivant : 000629

An experimental inoculation system to study citrus-Xylella fastidiosa interactions

Auteurs : S. A. Lopes ; D. C. Teixeira ; N. G. Fernandes ; A. J. Ayres ; S. C. Z. Torres ; J. C. Barbosa ; I. W. B. Li

Source :

Plant disease [ 0191-2917 ] ; 2005.

RBID : Pascal:05-0185753

Descripteurs français

Pascal (Inist)
- Citrus, Etude expérimentale, Microbiologie, Phytopathologie, Phytopathogène, Plante, Xylella fastidiosa.

English descriptors

KwdEn :
- Citrus, Experimental study, Microbiology, Plant, Plant pathogen, Plant pathology.

Abstract

Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0191-2917`
A02	`01`			`@0 PLDIDE`
A03		`1`		`@0 Plant dis.`
A05				`@2 89`
A06				`@2 3`
A08	`01`	`1`	`ENG`	`@1 An experimental inoculation system to study citrus-Xylella fastidiosa interactions`
A11	`01`	`1`		`@1 LOPES (S. A.)`
A11	`02`	`1`		`@1 TEIXEIRA (D. C.)`
A11	`03`	`1`		`@1 FERNANDES (N. G.)`
A11	`04`	`1`		`@1 AYRES (A. J.)`
A11	`05`	`1`		`@1 TORRES (S. C. Z.)`
A11	`06`	`1`		`@1 BARBOSA (J. C.)`
A11	`07`	`1`		`@1 LI (I. W. B.)`
A14	`01`			`@1 FUNDECITRUS @2 Araraquara, SP, 14801-970 @3 BRA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.`
A14	`02`			`@1 UNAERP, @2 Ribeirao Preto, SP, 14096-380 @3 BRA @Z 5 aut.`
A14	`03`			`@1 UNESP @2 Jaboticabal, SP, 14884-900 @3 BRA @Z 6 aut.`
A14	`04`			`@1 USDA-ARS @2 Beltsville, MD 20705-2350 @3 USA @Z 7 aut.`
A20				`@1 250-254`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 12673 @5 354000125080580060`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 16 ref.`
A47	`01`	`1`		`@0 05-0185753`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Plant disease`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.
C02	`01`	`X`		`@0 002A34`
C03	`01`	`X`	`FRE`	`@0 Citrus @2 NS @5 01`
C03	`01`	`X`	`ENG`	`@0 Citrus @2 NS @5 01`
C03	`01`	`X`	`SPA`	`@0 Citrus @2 NS @5 01`
C03	`02`	`X`	`FRE`	`@0 Etude expérimentale @5 05`
C03	`02`	`X`	`ENG`	`@0 Experimental study @5 05`
C03	`02`	`X`	`SPA`	`@0 Estudio experimental @5 05`
C03	`03`	`X`	`FRE`	`@0 Microbiologie @5 06`
C03	`03`	`X`	`ENG`	`@0 Microbiology @5 06`
C03	`03`	`X`	`SPA`	`@0 Microbiología @5 06`
C03	`04`	`X`	`FRE`	`@0 Phytopathologie @5 07`
C03	`04`	`X`	`ENG`	`@0 Plant pathology @5 07`
C03	`04`	`X`	`SPA`	`@0 Fitopatología @5 07`
C03	`05`	`X`	`FRE`	`@0 Phytopathogène @5 08`
C03	`05`	`X`	`ENG`	`@0 Plant pathogen @5 08`
C03	`05`	`X`	`SPA`	`@0 Fitopatógeno @5 08`
C03	`06`	`X`	`FRE`	`@0 Plante @5 45`
C03	`06`	`X`	`ENG`	`@0 Plant @5 45`
C03	`06`	`X`	`SPA`	`@0 Planta @5 45`
C03	`07`	`X`	`FRE`	`@0 Xylella fastidiosa @4 INC @5 79`
C07	`01`	`X`	`FRE`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`ENG`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`SPA`	`@0 Rutaceae @2 NS`
C07	`02`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`03`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
C07	`05`	`X`	`FRE`	`@0 Agrume @5 17`
C07	`05`	`X`	`ENG`	`@0 Citrus fruit @5 17`
C07	`05`	`X`	`SPA`	`@0 Agrios @5 17`
N21				`@1 122`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 05-0185753 INIST
ET :	An experimental inoculation system to study citrus-Xylella fastidiosa interactions
AU :	LOPES (S. A.); TEIXEIRA (D. C.); FERNANDES (N. G.); AYRES (A. J.); TORRES (S. C. Z.); BARBOSA (J. C.); LI (I. W. B.)
AF :	FUNDECITRUS/Araraquara, SP, 14801-970/Brésil (1 aut., 2 aut., 3 aut., 4 aut.); UNAERP,/Ribeirao Preto, SP, 14096-380/Brésil (5 aut.); UNESP/Jaboticabal, SP, 14884-900/Brésil (6 aut.); USDA-ARS/Beltsville, MD 20705-2350/Etats-Unis (7 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant disease; ISSN 0191-2917; Coden PLDIDE; Etats-Unis; Da. 2005; Vol. 89; No. 3; Pp. 250-254; Bibl. 16 ref.
LA :	Anglais
EA :	Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.
CC :	002A34
FD :	Citrus; Etude expérimentale; Microbiologie; Phytopathologie; Phytopathogène; Plante; Xylella fastidiosa
FG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume
ED :	Citrus; Experimental study; Microbiology; Plant pathology; Plant pathogen; Plant
EG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit
SD :	Citrus; Estudio experimental; Microbiología; Fitopatología; Fitopatógeno; Planta
LO :	INIST-12673.354000125080580060
ID :	05-0185753

Links to Exploration step

Pascal:05-0185753

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">An experimental inoculation system to study citrus-Xylella fastidiosa interactions</title>
<author><name sortKey="Lopes, S A" sort="Lopes, S A" uniqKey="Lopes S" first="S. A." last="Lopes">S. A. Lopes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Teixeira, D C" sort="Teixeira, D C" uniqKey="Teixeira D" first="D. C." last="Teixeira">D. C. Teixeira</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Fernandes, N G" sort="Fernandes, N G" uniqKey="Fernandes N" first="N. G." last="Fernandes">N. G. Fernandes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ayres, A J" sort="Ayres, A J" uniqKey="Ayres A" first="A. J." last="Ayres">A. J. Ayres</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Torres, S C Z" sort="Torres, S C Z" uniqKey="Torres S" first="S. C. Z." last="Torres">S. C. Z. Torres</name>
<affiliation><inist:fA14 i1="02"><s1>UNAERP,</s1>
<s2>Ribeirao Preto, SP, 14096-380</s2>
<s3>BRA</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Barbosa, J C" sort="Barbosa, J C" uniqKey="Barbosa J" first="J. C." last="Barbosa">J. C. Barbosa</name>
<affiliation><inist:fA14 i1="03"><s1>UNESP</s1>
<s2>Jaboticabal, SP, 14884-900</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Li, I W B" sort="Li, I W B" uniqKey="Li I" first="I. W. B." last="Li">I. W. B. Li</name>
<affiliation><inist:fA14 i1="04"><s1>USDA-ARS</s1>
<s2>Beltsville, MD 20705-2350</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">05-0185753</idno>
<date when="2005">2005</date>
<idno type="stanalyst">PASCAL 05-0185753 INIST</idno>
<idno type="RBID">Pascal:05-0185753</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000628</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">An experimental inoculation system to study citrus-Xylella fastidiosa interactions</title>
<author><name sortKey="Lopes, S A" sort="Lopes, S A" uniqKey="Lopes S" first="S. A." last="Lopes">S. A. Lopes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Teixeira, D C" sort="Teixeira, D C" uniqKey="Teixeira D" first="D. C." last="Teixeira">D. C. Teixeira</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Fernandes, N G" sort="Fernandes, N G" uniqKey="Fernandes N" first="N. G." last="Fernandes">N. G. Fernandes</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ayres, A J" sort="Ayres, A J" uniqKey="Ayres A" first="A. J." last="Ayres">A. J. Ayres</name>
<affiliation><inist:fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Torres, S C Z" sort="Torres, S C Z" uniqKey="Torres S" first="S. C. Z." last="Torres">S. C. Z. Torres</name>
<affiliation><inist:fA14 i1="02"><s1>UNAERP,</s1>
<s2>Ribeirao Preto, SP, 14096-380</s2>
<s3>BRA</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Barbosa, J C" sort="Barbosa, J C" uniqKey="Barbosa J" first="J. C." last="Barbosa">J. C. Barbosa</name>
<affiliation><inist:fA14 i1="03"><s1>UNESP</s1>
<s2>Jaboticabal, SP, 14884-900</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Li, I W B" sort="Li, I W B" uniqKey="Li I" first="I. W. B." last="Li">I. W. B. Li</name>
<affiliation><inist:fA14 i1="04"><s1>USDA-ARS</s1>
<s2>Beltsville, MD 20705-2350</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant disease</title>
<title level="j" type="abbreviated">Plant dis.</title>
<idno type="ISSN">0191-2917</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant disease</title>
<title level="j" type="abbreviated">Plant dis.</title>
<idno type="ISSN">0191-2917</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus</term>
<term>Experimental study</term>
<term>Microbiology</term>
<term>Plant</term>
<term>Plant pathogen</term>
<term>Plant pathology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Citrus</term>
<term>Etude expérimentale</term>
<term>Microbiologie</term>
<term>Phytopathologie</term>
<term>Phytopathogène</term>
<term>Plante</term>
<term>Xylella fastidiosa</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0191-2917</s0>
</fA01>
<fA02 i1="01"><s0>PLDIDE</s0>
</fA02>
<fA03 i2="1"><s0>Plant dis.</s0>
</fA03>
<fA05><s2>89</s2>
</fA05>
<fA06><s2>3</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>An experimental inoculation system to study citrus-Xylella fastidiosa interactions</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LOPES (S. A.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>TEIXEIRA (D. C.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>FERNANDES (N. G.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>AYRES (A. J.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>TORRES (S. C. Z.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BARBOSA (J. C.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>LI (I. W. B.)</s1>
</fA11>
<fA14 i1="01"><s1>FUNDECITRUS</s1>
<s2>Araraquara, SP, 14801-970</s2>
<s3>BRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>UNAERP,</s1>
<s2>Ribeirao Preto, SP, 14096-380</s2>
<s3>BRA</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>UNESP</s1>
<s2>Jaboticabal, SP, 14884-900</s2>
<s3>BRA</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>USDA-ARS</s1>
<s2>Beltsville, MD 20705-2350</s2>
<s3>USA</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA20><s1>250-254</s1>
</fA20>
<fA21><s1>2005</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>12673</s2>
<s5>354000125080580060</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2005 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>16 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>05-0185753</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Plant disease</s0>
</fA64>
<fA66 i1="01"><s0>USA</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A34</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Citrus</s0>
<s2>NS</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Citrus</s0>
<s2>NS</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Citrus</s0>
<s2>NS</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Etude expérimentale</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Experimental study</s0>
<s5>05</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Estudio experimental</s0>
<s5>05</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Microbiologie</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Microbiology</s0>
<s5>06</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Microbiología</s0>
<s5>06</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Phytopathologie</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Plant pathology</s0>
<s5>07</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Fitopatología</s0>
<s5>07</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Phytopathogène</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Plant pathogen</s0>
<s5>08</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Fitopatógeno</s0>
<s5>08</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Plante</s0>
<s5>45</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Plant</s0>
<s5>45</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Planta</s0>
<s5>45</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Xylella fastidiosa</s0>
<s4>INC</s4>
<s5>79</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Agrume</s0>
<s5>17</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>17</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Agrios</s0>
<s5>17</s5>
</fC07>
<fN21><s1>122</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 05-0185753 INIST</NO>
<ET>An experimental inoculation system to study citrus-Xylella fastidiosa interactions</ET>
<AU>LOPES (S. A.); TEIXEIRA (D. C.); FERNANDES (N. G.); AYRES (A. J.); TORRES (S. C. Z.); BARBOSA (J. C.); LI (I. W. B.)</AU>
<AF>FUNDECITRUS/Araraquara, SP, 14801-970/Brésil (1 aut., 2 aut., 3 aut., 4 aut.); UNAERP,/Ribeirao Preto, SP, 14096-380/Brésil (5 aut.); UNESP/Jaboticabal, SP, 14884-900/Brésil (6 aut.); USDA-ARS/Beltsville, MD 20705-2350/Etats-Unis (7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant disease; ISSN 0191-2917; Coden PLDIDE; Etats-Unis; Da. 2005; Vol. 89; No. 3; Pp. 250-254; Bibl. 16 ref.</SO>
<LA>Anglais</LA>
<EA>Difficulties in reproducing the citrus variegated chlorosis (CVC) disease symptoms in experimental plants have delayed implementation of studies to better understand the essential aspects of this important disease. In an extensive study, cultivars of sweet orange (Citrus sinensis) were inoculated with Xylella fastidiosa using procedures that included root immersion, and stem absorption, pricking, or infiltration of the inoculum into plants of different ages. Inoculum consisted of 5-day-old cultures or cell suspensions of CVC strain 9a5c diluted in phosphate-buffered saline. Inoculated plants and controls were grown, or transferred just after inoculation, to 5-liter pots or 72-cell foam trays. Approximately 4, 5, 9, and 12 months after inoculation, leaves were collected and processed for polymerase chain reaction analysis or X. fastidiosa isolation on BCYE agar medium. Root immersion and stem inoculation of 4- and 6-month-old plants resulted in low percentages of symptomatic (0 to 7%) and plants positive by isolation (0 to 9%). Pinpricked or injected stems of 1-month-old seedlings resulted in high percentages of plants symptomatic (29 and 90% in Pera Rio, 75, 59, and 83% in Valencia, and 77% in Natal) or positive by isolation (26 and 93% in Pera Rio, 98, 96, and 83% in Valencia, and 77% in Natal). In foam trays, the seedlings grew less, the incubation period was shorter, and disease severity was higher than in pots. This system allows testing of higher numbers of plants in a reduced space with a more precise reproduction of the experimental conditions.</EA>
<CC>002A34</CC>
<FD>Citrus; Etude expérimentale; Microbiologie; Phytopathologie; Phytopathogène; Plante; Xylella fastidiosa</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume</FG>
<ED>Citrus; Experimental study; Microbiology; Plant pathology; Plant pathogen; Plant</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit</EG>
<SD>Citrus; Estudio experimental; Microbiología; Fitopatología; Fitopatógeno; Planta</SD>
<LO>INIST-12673.354000125080580060</LO>
<ID>05-0185753</ID>
</server>
</inist>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PascalFrancis/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000628 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Corpus/biblio.hfd -nk 000628 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    PascalFrancis
   |étape=   Corpus
   |type=    RBID
   |clé=     Pascal:05-0185753
   |texte=   An experimental inoculation system to study citrus-Xylella fastidiosa interactions
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

An experimental inoculation system to study citrus-Xylella fastidiosa interactions

An experimental inoculation system to study citrus-Xylella fastidiosa interactions

Source :

Descripteurs français

English descriptors

Abstract

Notice en format standard (ISO 2709)

Format Inist (serveur)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri