OrangerV1, PascalFrancis, Corpus, bibRecord, 000507

Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange

Identifieur interne : 000507 ( PascalFrancis/Corpus ); précédent : 000506; suivant : 000508

Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange

Auteurs : W. W. Guo ; R. C. Wu ; Y. J. Cheng ; X. X. Deng

Source :

Plant breeding [ 0179-9541 ] ; 2007.

RBID : Pascal:07-0131645

Descripteurs français

Pascal (Inist)
- Paramètre moléculaire, Hybridation intergénérique, Hybride somatique, Protoplaste, DNA microsatellite, Cytoplasme, Génome, Amélioration génétique, Citrus reticulata, Citroncirus webberi, Citrange.

English descriptors

KwdEn :
- Citrange, Citroncirus webberi, Citrus reticulata, Cytoplasm, Genetic improvement, Genome, Intergeneric hybridization, Microsatellite DNA, Molecular parameter, Protoplast, Somatic hybrid.

Abstract

Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis × P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slowly and were vulnerable to browning. Twelve plants were finally regenerated, rooted and transplanted into a greenhouse. Root-tip chromosome counting of five randomly-selected plants revealed most cells were tetraploid (2n = 4x = 36), but aneuploid cells also existed. Flow cytometry analysis further confirmed their tetraploid nature. Nuclear simple sequence repeat (SSR) analysis verified their hybridity. Further mitochondrial genome analysis by restriction fragment length polymorphism and cleaved amplified polymorphic sequence revealed their mtDNA banding pattern was identical to that of red tangerine, the embryogenic callus parent; while their chloroplast DNA inheritance was random as revealed by chloroplast SSR analysis, in addition to cpDNA co-existence detected in one plant. Cytological and molecular analysis indicated that somatic hybrid plants between red tangerine and citrange had been successfully obtained.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`03`	`1`		`@1 CHENG (Y. J.)`
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A14	`01`			`@1 National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University @2 Wuhan 430070 @3 CHN @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut.`
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C01	`01`		`ENG`	@0 Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis × P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slowly and were vulnerable to browning. Twelve plants were finally regenerated, rooted and transplanted into a greenhouse. Root-tip chromosome counting of five randomly-selected plants revealed most cells were tetraploid (2n = 4x = 36), but aneuploid cells also existed. Flow cytometry analysis further confirmed their tetraploid nature. Nuclear simple sequence repeat (SSR) analysis verified their hybridity. Further mitochondrial genome analysis by restriction fragment length polymorphism and cleaved amplified polymorphic sequence revealed their mtDNA banding pattern was identical to that of red tangerine, the embryogenic callus parent; while their chloroplast DNA inheritance was random as revealed by chloroplast SSR analysis, in addition to cpDNA co-existence detected in one plant. Cytological and molecular analysis indicated that somatic hybrid plants between red tangerine and citrange had been successfully obtained.
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Format Inist (serveur)

NO :	PASCAL 07-0131645 INIST
ET :	Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange
AU :	GUO (W. W.); WU (R. C.); CHENG (Y. J.); DENG (X. X.)
AF :	National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University/Wuhan 430070/Chine (1 aut., 2 aut., 3 aut., 4 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant breeding; ISSN 0179-9541; Coden PLABED; Allemagne; Da. 2007; Vol. 126; No. 1; Pp. 72-76; Bibl. 3/4 p.
LA :	Anglais
EA :	Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis × P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slowly and were vulnerable to browning. Twelve plants were finally regenerated, rooted and transplanted into a greenhouse. Root-tip chromosome counting of five randomly-selected plants revealed most cells were tetraploid (2n = 4x = 36), but aneuploid cells also existed. Flow cytometry analysis further confirmed their tetraploid nature. Nuclear simple sequence repeat (SSR) analysis verified their hybridity. Further mitochondrial genome analysis by restriction fragment length polymorphism and cleaved amplified polymorphic sequence revealed their mtDNA banding pattern was identical to that of red tangerine, the embryogenic callus parent; while their chloroplast DNA inheritance was random as revealed by chloroplast SSR analysis, in addition to cpDNA co-existence detected in one plant. Cytological and molecular analysis indicated that somatic hybrid plants between red tangerine and citrange had been successfully obtained.
CC :	002A32D02A; 002A32D02D
FD :	Paramètre moléculaire; Hybridation intergénérique; Hybride somatique; Protoplaste; DNA microsatellite; Cytoplasme; Génome; Amélioration génétique; Citrus reticulata; Citroncirus webberi; Citrange
FG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Porte greffe
ED :	Molecular parameter; Intergeneric hybridization; Somatic hybrid; Protoplast; Microsatellite DNA; Cytoplasm; Genome; Genetic improvement; Citrus reticulata; Citroncirus webberi; Citrange
EG :	Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Rootstock
SD :	Parámetro molecular; Hibridacion intergenérica; Híbrido somático; Protoplasto; DNA microsatélite; Citoplasma; Genoma; Enriquecimiento genético; Citrus reticulata; Citroncirus webberi; Citranjo
LO :	INIST-876.354000145501160150
ID :	07-0131645

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Pascal:07-0131645

Le document en format XML

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<front><div type="abstract" xml:lang="en">Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis × P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slowly and were vulnerable to browning. Twelve plants were finally regenerated, rooted and transplanted into a greenhouse. Root-tip chromosome counting of five randomly-selected plants revealed most cells were tetraploid (2n = 4x = 36), but aneuploid cells also existed. Flow cytometry analysis further confirmed their tetraploid nature. Nuclear simple sequence repeat (SSR) analysis verified their hybridity. Further mitochondrial genome analysis by restriction fragment length polymorphism and cleaved amplified polymorphic sequence revealed their mtDNA banding pattern was identical to that of red tangerine, the embryogenic callus parent; while their chloroplast DNA inheritance was random as revealed by chloroplast SSR analysis, in addition to cpDNA co-existence detected in one plant. Cytological and molecular analysis indicated that somatic hybrid plants between red tangerine and citrange had been successfully obtained.</div>
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<fC01 i1="01" l="ENG"><s0>Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis × P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slowly and were vulnerable to browning. Twelve plants were finally regenerated, rooted and transplanted into a greenhouse. Root-tip chromosome counting of five randomly-selected plants revealed most cells were tetraploid (2n = 4x = 36), but aneuploid cells also existed. Flow cytometry analysis further confirmed their tetraploid nature. Nuclear simple sequence repeat (SSR) analysis verified their hybridity. Further mitochondrial genome analysis by restriction fragment length polymorphism and cleaved amplified polymorphic sequence revealed their mtDNA banding pattern was identical to that of red tangerine, the embryogenic callus parent; while their chloroplast DNA inheritance was random as revealed by chloroplast SSR analysis, in addition to cpDNA co-existence detected in one plant. Cytological and molecular analysis indicated that somatic hybrid plants between red tangerine and citrange had been successfully obtained.</s0>
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<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>DNA microsatélite</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Cytoplasme</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Cytoplasm</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Citoplasma</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Génome</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Genome</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Genoma</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Amélioration génétique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Genetic improvement</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Enriquecimiento genético</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Citrus reticulata</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Citrus reticulata</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Citrus reticulata</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Citroncirus webberi</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Citroncirus webberi</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Citroncirus webberi</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Citrange</s0>
<s4>CD</s4>
<s5>97</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Citrange</s0>
<s4>CD</s4>
<s5>97</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Citranjo</s0>
<s4>CD</s4>
<s5>97</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Agrume</s0>
<s5>31</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>31</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Agrios</s0>
<s5>31</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Porte greffe</s0>
<s5>32</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Rootstock</s0>
<s5>32</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Portainjerto</s0>
<s5>32</s5>
</fC07>
<fN21><s1>085</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 07-0131645 INIST</NO>
<ET>Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange</ET>
<AU>GUO (W. W.); WU (R. C.); CHENG (Y. J.); DENG (X. X.)</AU>
<AF>National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University/Wuhan 430070/Chine (1 aut., 2 aut., 3 aut., 4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant breeding; ISSN 0179-9541; Coden PLABED; Allemagne; Da. 2007; Vol. 126; No. 1; Pp. 72-76; Bibl. 3/4 p.</SO>
<LA>Anglais</LA>
<EA>Somatic hybridization has been an effective and successful technique for plant improvement. In this paper, embryogenic callus protoplasts of red tangerine (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts from citrange (C. sinensis × P. trifoliata, a Chinese local strain) in an effort to produce complementary tetraploid citrus rootstocks. Regenerated embryoids grew slowly and were vulnerable to browning. Twelve plants were finally regenerated, rooted and transplanted into a greenhouse. Root-tip chromosome counting of five randomly-selected plants revealed most cells were tetraploid (2n = 4x = 36), but aneuploid cells also existed. Flow cytometry analysis further confirmed their tetraploid nature. Nuclear simple sequence repeat (SSR) analysis verified their hybridity. Further mitochondrial genome analysis by restriction fragment length polymorphism and cleaved amplified polymorphic sequence revealed their mtDNA banding pattern was identical to that of red tangerine, the embryogenic callus parent; while their chloroplast DNA inheritance was random as revealed by chloroplast SSR analysis, in addition to cpDNA co-existence detected in one plant. Cytological and molecular analysis indicated that somatic hybrid plants between red tangerine and citrange had been successfully obtained.</EA>
<CC>002A32D02A; 002A32D02D</CC>
<FD>Paramètre moléculaire; Hybridation intergénérique; Hybride somatique; Protoplaste; DNA microsatellite; Cytoplasme; Génome; Amélioration génétique; Citrus reticulata; Citroncirus webberi; Citrange</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Porte greffe</FG>
<ED>Molecular parameter; Intergeneric hybridization; Somatic hybrid; Protoplast; Microsatellite DNA; Cytoplasm; Genome; Genetic improvement; Citrus reticulata; Citroncirus webberi; Citrange</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Rootstock</EG>
<SD>Parámetro molecular; Hibridacion intergenérica; Híbrido somático; Protoplasto; DNA microsatélite; Citoplasma; Genoma; Enriquecimiento genético; Citrus reticulata; Citroncirus webberi; Citranjo</SD>
<LO>INIST-876.354000145501160150</LO>
<ID>07-0131645</ID>
</server>
</inist>
</record>

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Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange

Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange

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