OrangerV1, PascalFrancis, Corpus, bibRecord, 000482

Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

Identifieur interne : 000482 ( PascalFrancis/Corpus ); précédent : 000481; suivant : 000483

Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

Auteurs : Alida Ballester ; Magdalena Cervera ; Leandro Pena

Source :

Plant cell reports : (Print) [ 0721-7714 ] ; 2007.

RBID : Pascal:07-0285340

Descripteurs français

Pascal (Inist)
- Production, Plante transgénique, Transferases, Sélection, Gène, Citrus, Site spécifique, Recombinaison, Transformation génétique, Végétal, Citrus sinensis Poncirus trifoliata, Isopentenyl transferase.

English descriptors

KwdEn :
- Citrus, Gene, Genetic transformation, Production, Recombination, Selection, Site specificity, Transferases, Transgenic plant, Vegetals.

Abstract

The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptll, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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C01	`01`		`ENG`	@0 The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptll, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.
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Format Inist (serveur)

NO :	PASCAL 07-0285340 INIST
ET :	Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination
AU :	BALLESTER (Alida); CERVERA (Magdalena); PENA (Leandro)
AF :	Department Plant Protection and Biotechnology, Institute Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial/46113-Moncada, Valencia/Espagne (1 aut., 2 aut., 3 aut.)
DT :	Publication en série; Niveau analytique
SO :	Plant cell reports : (Print); ISSN 0721-7714; Coden PCRPD8; Allemagne; Da. 2007; Vol. 26; No. 1; Pp. 39-45; Bibl. 19 ref.
LA :	Anglais
EA :	The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptll, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.
CC :	002A31C02A5B; 215
FD :	Production; Plante transgénique; Transferases; Sélection; Gène; Citrus; Site spécifique; Recombinaison; Transformation génétique; Végétal; Citrus sinensis Poncirus trifoliata; Isopentenyl transferase
FG :	Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
ED :	Production; Transgenic plant; Transferases; Selection; Gene; Citrus; Site specificity; Recombination; Genetic transformation; Vegetals
EG :	Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
SD :	Producción; Planta transgénica; Transferases; Selección; Gen; Citrus; Sitio específico; Recombinación; Transformación genética; Vegetal
LO :	INIST-18737.354000159654870050
ID :	07-0285340

Links to Exploration step

Pascal:07-0285340

Le document en format XML

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<front><div type="abstract" xml:lang="en">The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptll, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.</div>
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<s5>91</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Isopentenyl transferase</s0>
<s4>INC</s4>
<s5>92</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
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<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="04" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fN21><s1>190</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 07-0285340 INIST</NO>
<ET>Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination</ET>
<AU>BALLESTER (Alida); CERVERA (Magdalena); PENA (Leandro)</AU>
<AF>Department Plant Protection and Biotechnology, Institute Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial/46113-Moncada, Valencia/Espagne (1 aut., 2 aut., 3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Plant cell reports : (Print); ISSN 0721-7714; Coden PCRPD8; Allemagne; Da. 2007; Vol. 26; No. 1; Pp. 39-45; Bibl. 19 ref.</SO>
<LA>Anglais</LA>
<EA>The presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptll, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.</EA>
<CC>002A31C02A5B; 215</CC>
<FD>Production; Plante transgénique; Transferases; Sélection; Gène; Citrus; Site spécifique; Recombinaison; Transformation génétique; Végétal; Citrus sinensis  Poncirus trifoliata; Isopentenyl transferase</FD>
<FG>Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Production; Transgenic plant; Transferases; Selection; Gene; Citrus; Site specificity; Recombination; Genetic transformation; Vegetals</ED>
<EG>Enzyme; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Producción; Planta transgénica; Transferases; Selección; Gen; Citrus; Sitio específico; Recombinación; Transformación genética; Vegetal</SD>
<LO>INIST-18737.354000159654870050</LO>
<ID>07-0285340</ID>
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Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination

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