A simple and efficient cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck.] by encapsulation-vitrification
Identifieur interne : 000826 ( PascalFrancis/Checkpoint ); précédent : 000825; suivant : 000827A simple and efficient cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck.] by encapsulation-vitrification
Auteurs : QIAOCHUN WANG [Israël] ; Özgur Batuman [Israël] ; PING LI [Israël] ; Moshe Bar-Joseph [Israël] ; Ron Gafny [Israël]Source :
- Euphytica [ 0014-2336 ] ; 2002.
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Abstract
A simple and efficient method was developed for cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange by encapsulation-vitrification. Excised shoot tips were precultured with increasing sucrose concentrations of 0.3, 0.5, 0.75 and 1 M for 4 days. Precultured shoot tips were encapsulated and simultaneously osmoprotected with a loading solution of 2 M glycerol and 1 M sucrose. Osmoprotected and encapsulated shoot tips were dehydrated with a highly concentrated vitrification solution prior to direct immersion in LN for 1 h. Optimal survival of cryopreserved shoot tips was obtained when precultured shoot tips were osmoprotected for 60 min during encapsulation. Dehydration by exposure to modified PVS2 solution for 90 min at 24 °C and for 180 to 210 min at 0 °C was found optimal for survival of cryopreserved shoot tips. Preculture duration largely influenced survival of cryopreserved shoot tips, with the best result obtained after 2 to 5 days of preculture with stable 1 M sucrose. With the optimized parameters, 100% survival of cryopreserved shoot tips was achieved. Morphologies of plants regenerated from cryopreserved shoot tips were similar to those of the seedlings.
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Osbeck.] by encapsulation-vitrification</title><author><name sortKey="Qiaochun Wang" sort="Qiaochun Wang" uniqKey="Qiaochun Wang" last="Qiaochun Wang">QIAOCHUN WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Batuman, Ozgur" sort="Batuman, Ozgur" uniqKey="Batuman O" first="Özgur" last="Batuman">Özgur Batuman</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Ping Li" sort="Ping Li" uniqKey="Ping Li" last="Ping Li">PING LI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Bar Joseph, Moshe" sort="Bar Joseph, Moshe" uniqKey="Bar Joseph M" first="Moshe" last="Bar-Joseph">Moshe Bar-Joseph</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Gafny, Ron" sort="Gafny, Ron" uniqKey="Gafny R" first="Ron" last="Gafny">Ron Gafny</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">03-0155736</idno><date when="2002">2002</date><idno type="stanalyst">PASCAL 03-0155736 INIST</idno><idno type="RBID">Pascal:03-0155736</idno><idno type="wicri:Area/PascalFrancis/Corpus">000779</idno><idno type="wicri:Area/PascalFrancis/Curation">000225</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000826</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">A simple and efficient cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [Poncirus trifoliata (L.) 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Osbeck.] by encapsulation-vitrification</title><author><name sortKey="Qiaochun Wang" sort="Qiaochun Wang" uniqKey="Qiaochun Wang" last="Qiaochun Wang">QIAOCHUN WANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Batuman, Ozgur" sort="Batuman, Ozgur" uniqKey="Batuman O" first="Özgur" last="Batuman">Özgur Batuman</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Ping Li" sort="Ping Li" uniqKey="Ping Li" last="Ping Li">PING LI</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Bar Joseph, Moshe" sort="Bar Joseph, Moshe" uniqKey="Bar Joseph M" first="Moshe" last="Bar-Joseph">Moshe Bar-Joseph</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Gafny, Ron" sort="Gafny, Ron" uniqKey="Gafny R" first="Ron" last="Gafny">Ron Gafny</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, Agricultural Research Organization, the Volcani Center, P.O.B. 6</s1><s2>Bet Dagan 50250</s2><s3>ISR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Israël</country><wicri:noRegion>Bet Dagan 50250</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Euphytica</title><title level="j" type="abbreviated">Euphytica</title><idno type="ISSN">0014-2336</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Euphytica</title><title level="j" type="abbreviated">Euphytica</title><idno type="ISSN">0014-2336</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apex</term><term>Cryopreservation</term><term>Encapsulation</term><term>Gene bank</term><term>Microplant</term><term>Micropropagation</term><term>Osmoregulation</term><term>Vitrification</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cryoconservation</term><term>Apex</term><term>Encapsulation</term><term>Vitrification</term><term>Banque gène</term><term>Multiplication végétative in vitro</term><term>Osmorégulation</term><term>Vitroplant</term><term>Citrus sinensis Poncirus trifoliata</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A simple and efficient method was developed for cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange by encapsulation-vitrification. 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Morphologies of plants regenerated from cryopreserved shoot tips were similar to those of the seedlings.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0014-2336</s0></fA01><fA02 i1="01"><s0>EUPHAA</s0></fA02><fA03 i2="1"><s0>Euphytica</s0></fA03><fA05><s2>128</s2></fA05><fA06><s2>1</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>A simple and efficient cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) 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Dehydration by exposure to modified PVS2 solution for 90 min at 24 °C and for 180 to 210 min at 0 °C was found optimal for survival of cryopreserved shoot tips. Preculture duration largely influenced survival of cryopreserved shoot tips, with the best result obtained after 2 to 5 days of preculture with stable 1 M sucrose. With the optimized parameters, 100% survival of cryopreserved shoot tips was achieved. 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