The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)
Identifieur interne : 000697 ( PascalFrancis/Checkpoint ); précédent : 000696; suivant : 000698The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)
Auteurs : R. L. Boscariol [Brésil] ; W. A. B. Almeida [Brésil] ; M. T. V. C. Derbyshire [Brésil] ; F. A. A. Mourao Filho [Brésil] ; B. M. J. Mendes [Brésil]Source :
- Plant cell reports : (Print) [ 0721-7714 ] ; 2003.
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- topic : Plante transgénique, Saccharose.
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Abstract
A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.
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Boscariol</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13</s1><s2>400-970 São Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Almeida, W A B" sort="Almeida, W A B" uniqKey="Almeida W" first="W. A. B." last="Almeida">W. A. B. 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Mendes</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13</s1><s2>400-970 São Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0049994</idno><date when="2003">2003</date><idno type="stanalyst">PASCAL 04-0049994 INIST</idno><idno type="RBID">Pascal:04-0049994</idno><idno type="wicri:Area/PascalFrancis/Corpus">000716</idno><idno type="wicri:Area/PascalFrancis/Curation">000288</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000697</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)</title><author><name sortKey="Boscariol, R L" sort="Boscariol, R L" uniqKey="Boscariol R" first="R. L." last="Boscariol">R. L. Boscariol</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13</s1><s2>400-970 São Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Almeida, W A B" sort="Almeida, W A B" uniqKey="Almeida W" first="W. A. B." last="Almeida">W. A. B. Almeida</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departamento de Produçâo Vegetal, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba 13,</s1><s2>418-900 Sâo Paulo</s2><s3>BRA</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Brésil</country><wicri:noRegion>418-900 Sâo Paulo</wicri:noRegion><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Derbyshire, M T V C" sort="Derbyshire, M T V C" uniqKey="Derbyshire M" first="M. T. V. C." last="Derbyshire">M. T. V. C. Derbyshire</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Laboratório de Melhoramento de Plantas, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13</s1><s2>400-970 São Paulo</s2><s3>BRA</s3><sZ>3 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F. A. A." last="Mourao Filho">F. A. A. Mourao Filho</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departamento de Produçâo Vegetal, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba 13,</s1><s2>418-900 Sâo Paulo</s2><s3>BRA</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Brésil</country><wicri:noRegion>418-900 Sâo Paulo</wicri:noRegion><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B. M. J." last="Mendes">B. M. J. Mendes</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13</s1><s2>400-970 São Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Plant cell reports : (Print)</title><title level="j" type="abbreviated">Plant cell rep. : (Print)</title><idno type="ISSN">0721-7714</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant cell reports : (Print)</title><title level="j" type="abbreviated">Plant cell rep. : (Print)</title><idno type="ISSN">0721-7714</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Chemical composition</term><term>Citrus sinensis</term><term>Cultivar</term><term>Culture medium</term><term>Intraspecific comparison</term><term>Mannose</term><term>Mannose-6-phosphate isomerase</term><term>Marker assisted selection</term><term>Micropropagation</term><term>Sucrose</term><term>Transgenic plant</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Mannose-6-phosphate isomerase</term><term>Sélection assistée marqueur</term><term>Plante transgénique</term><term>Multiplication végétative in vitro</term><term>Composition chimique</term><term>Milieu culture</term><term>Comparaison intraspécifique</term><term>Cultivar</term><term>Citrus sinensis</term><term>Agrobacterium tumefaciens</term><term>Mannose</term><term>Saccharose</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term><term>Saccharose</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0721-7714</s0></fA01><fA02 i1="01"><s0>PCRPD8</s0></fA02><fA03 i2="1"><s0>Plant cell rep. : (Print)</s0></fA03><fA05><s2>22</s2></fA05><fA06><s2>2</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck)</s1></fA08><fA11 i1="01" i2="1"><s1>BOSCARIOL (R. L.)</s1></fA11><fA11 i1="02" i2="1"><s1>ALMEIDA (W. A. B.)</s1></fA11><fA11 i1="03" i2="1"><s1>DERBYSHIRE (M. T. V. C.)</s1></fA11><fA11 i1="04" i2="1"><s1>MOURAO FILHO (F. A. A.)</s1></fA11><fA11 i1="05" i2="1"><s1>MENDES (B. M. J.)</s1></fA11><fA14 i1="01"><s1>Laboratório de Biotecnologia Vegetal, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13</s1><s2>400-970 São Paulo</s2><s3>BRA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></fA14><fA14 i1="02"><s1>Departamento de Produçâo Vegetal, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba 13,</s1><s2>418-900 Sâo Paulo</s2><s3>BRA</s3><sZ>2 aut.</sZ><sZ>4 aut.</sZ></fA14><fA14 i1="03"><s1>Laboratório de Melhoramento de Plantas, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba 13</s1><s2>400-970 São Paulo</s2><s3>BRA</s3><sZ>3 aut.</sZ></fA14><fA20><s1>122-128</s1></fA20><fA21><s1>2003</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>18737</s2><s5>354000113259340060</s5></fA43><fA44><s0>0000</s0><s1>© 2004 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>43 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>04-0049994</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Plant cell reports : (Print)</s0></fA64><fA66 i1="01"><s0>DEU</s0></fA66><fC01 i1="01" l="ENG"><s0>A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides.</s0></fC01><fC02 i1="01" i2="X"><s0>002A32D02B</s0></fC02><fC02 i1="02" i2="X"><s0>002A31C02A5B</s0></fC02><fC02 i1="03" i2="X"><s0>215</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Mannose-6-phosphate isomerase</s0><s2>FE</s2><s5>01</s5><s6>Mannose«-6-»phosphate isomerase</s6></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Mannose-6-phosphate isomerase</s0><s2>FE</s2><s5>01</s5><s6>Mannose«-6-»phosphate isomerase</s6></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Mannose-6-phosphate isomerase</s0><s2>FE</s2><s5>01</s5><s6>Mannose«-6-»phosphate isomerase</s6></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Sélection assistée marqueur</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Marker assisted selection</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Selección asistida marcador</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Plante 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l="SPA"><s0>Manosa</s0><s2>NK</s2><s5>15</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Saccharose</s0><s2>NK</s2><s2>FR</s2><s5>16</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Sucrose</s0><s2>NK</s2><s2>FR</s2><s5>16</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Sacarosa</s0><s2>NK</s2><s2>FR</s2><s5>16</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Intramolecular oxidoreductases</s0><s2>FE</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Intramolecular oxidoreductases</s0><s2>FE</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Intramolecular oxidoreductases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Isomerases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Isomerases</s0><s2>FE</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Isomerases</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Enzyme</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Enzyme</s0><s2>FE</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Enzima</s0><s2>FE</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Rutaceae</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Rutaceae</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Rutaceae</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="FRE"><s0>Dicotyledones</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="ENG"><s0>Dicotyledones</s0><s2>NS</s2></fC07><fC07 i1="05" i2="X" l="SPA"><s0>Dicotyledones</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="FRE"><s0>Angiospermae</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="ENG"><s0>Angiospermae</s0><s2>NS</s2></fC07><fC07 i1="06" i2="X" l="SPA"><s0>Angiospermae</s0><s2>NS</s2></fC07><fC07 i1="07" i2="X" l="FRE"><s0>Spermatophyta</s0><s2>NS</s2></fC07><fC07 i1="07" i2="X" l="ENG"><s0>Spermatophyta</s0><s2>NS</s2></fC07><fC07 i1="07" i2="X" l="SPA"><s0>Spermatophyta</s0><s2>NS</s2></fC07><fC07 i1="08" i2="X" l="FRE"><s0>Rhizobiaceae</s0><s2>NS</s2></fC07><fC07 i1="08" i2="X" l="ENG"><s0>Rhizobiaceae</s0><s2>NS</s2></fC07><fC07 i1="08" i2="X" l="SPA"><s0>Rhizobiaceae</s0><s2>NS</s2></fC07><fC07 i1="09" i2="X" l="FRE"><s0>Bactérie</s0></fC07><fC07 i1="09" i2="X" l="ENG"><s0>Bacteria</s0></fC07><fC07 i1="09" i2="X" l="SPA"><s0>Bacteria</s0></fC07><fC07 i1="10" i2="X" l="FRE"><s0>Marqueur génétique</s0><s5>33</s5></fC07><fC07 i1="10" i2="X" l="ENG"><s0>Genetic marker</s0><s5>33</s5></fC07><fC07 i1="10" i2="X" l="SPA"><s0>Marcador genético</s0><s5>33</s5></fC07><fC07 i1="11" i2="X" l="FRE"><s0>Agrume</s0><s5>39</s5></fC07><fC07 i1="11" i2="X" l="ENG"><s0>Citrus fruit</s0><s5>39</s5></fC07><fC07 i1="11" i2="X" l="SPA"><s0>Agrios</s0><s5>39</s5></fC07><fC07 i1="12" i2="X" l="FRE"><s0>Phytopathogène</s0><s5>41</s5></fC07><fC07 i1="12" i2="X" l="ENG"><s0>Plant pathogen</s0><s5>41</s5></fC07><fC07 i1="12" i2="X" l="SPA"><s0>Fitopatógeno</s0><s5>41</s5></fC07><fN21><s1>033</s1></fN21><fN82><s1>PSI</s1></fN82></pA></standard></inist><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Boscariol, R L" sort="Boscariol, R L" uniqKey="Boscariol R" first="R. L." last="Boscariol">R. L. Boscariol</name></region><name sortKey="Almeida, W A B" sort="Almeida, W A B" uniqKey="Almeida W" first="W. A. B." last="Almeida">W. A. B. Almeida</name><name sortKey="Derbyshire, M T V C" sort="Derbyshire, M T V C" uniqKey="Derbyshire M" first="M. T. V. C." last="Derbyshire">M. T. V. C. Derbyshire</name><name sortKey="Mendes, B M J" sort="Mendes, B M J" uniqKey="Mendes B" first="B. M. J." last="Mendes">B. M. J. Mendes</name><name sortKey="Mourao Filho, F A A" sort="Mourao Filho, F A A" uniqKey="Mourao Filho F" first="F. A. A." last="Mourao Filho">F. A. A. Mourao Filho</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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