The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding.
Identifieur interne : 002261 ( Ncbi/Merge ); précédent : 002260; suivant : 002262The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding.
Auteurs : M L Roose [Royaume-Uni] ; T. Schwarzacher ; J S Heslop-HarrisonSource :
- The Journal of heredity [ 0022-1503 ]
English descriptors
- KwdEn :
- MESH :
- chemical : DNA, Ribosomal, Fluorescent Dyes, Indoles, RNA, Ribosomal, RNA, Ribosomal, 18S, RNA, Ribosomal, 5S.
- genetics : Citrus.
- methods : Chromosome Banding, In Situ Hybridization.
- Chromosome Mapping, Chromosomes.
Abstract
In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x = 18), Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus x Poncirus hybrids (2n = 2x = 18). Counterstaining with the fluorochromes chromomycin A3 and DAPI uniquely identified many but not all chromosomes. C. sinensis had five 18S-25S rDNA sites, P. trifoliata had seven, and three different Citrus x Poncirus hybrids had five or six sites. Four 5S rDNA sites were detected, mostly linked to 18S-25S rDNA sites. Overall we observed high levels of chromosomal heterozygosity in all accessions examined.
PubMed: 9487679
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pubmed:9487679Le document en format XML
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Schwarzacher</name></author><author><name sortKey="Heslop Harrison, J S" sort="Heslop Harrison, J S" uniqKey="Heslop Harrison J" first="J S" last="Heslop-Harrison">J S Heslop-Harrison</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>1998 Jan-Feb</MedlineDate></PubDate></date><idno type="RBID">pubmed:9487679</idno><idno type="pmid">9487679</idno><idno type="wicri:Area/PubMed/Corpus">000F27</idno><idno type="wicri:Area/PubMed/Curation">000F27</idno><idno type="wicri:Area/PubMed/Checkpoint">000F27</idno><idno type="wicri:Area/Ncbi/Merge">002261</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding.</title><author><name sortKey="Roose, M L" sort="Roose, M L" uniqKey="Roose M" first="M L" last="Roose">M L Roose</name><affiliation wicri:level="2"><nlm:affiliation>Department of Cell Biology, John Innes Centre, Norwich, England.</nlm:affiliation><country>Royaume-Uni</country><placeName><region type="country">Angleterre</region></placeName><wicri:cityArea>Department of Cell Biology, John Innes Centre, Norwich</wicri:cityArea></affiliation></author><author><name sortKey="Schwarzacher, T" sort="Schwarzacher, T" uniqKey="Schwarzacher T" first="T" last="Schwarzacher">T. Schwarzacher</name></author><author><name sortKey="Heslop Harrison, J S" sort="Heslop Harrison, J S" uniqKey="Heslop Harrison J" first="J S" last="Heslop-Harrison">J S Heslop-Harrison</name></author></analytic><series><title level="j">The Journal of heredity</title><idno type="ISSN">0022-1503</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosome Banding (methods)</term><term>Chromosome Mapping</term><term>Chromosomes</term><term>Citrus (genetics)</term><term>DNA, Ribosomal</term><term>Fluorescent Dyes</term><term>In Situ Hybridization (methods)</term><term>Indoles</term><term>RNA, Ribosomal</term><term>RNA, Ribosomal, 18S</term><term>RNA, Ribosomal, 5S</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Ribosomal</term><term>Fluorescent Dyes</term><term>Indoles</term><term>RNA, Ribosomal</term><term>RNA, Ribosomal, 18S</term><term>RNA, Ribosomal, 5S</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromosome Banding</term><term>In Situ Hybridization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term><term>Chromosomes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x = 18), Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus x Poncirus hybrids (2n = 2x = 18). Counterstaining with the fluorochromes chromomycin A3 and DAPI uniquely identified many but not all chromosomes. C. sinensis had five 18S-25S rDNA sites, P. trifoliata had seven, and three different Citrus x Poncirus hybrids had five or six sites. Four 5S rDNA sites were detected, mostly linked to 18S-25S rDNA sites. Overall we observed high levels of chromosomal heterozygosity in all accessions examined.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9487679</PMID><DateCreated><Year>1998</Year><Month>3</Month><Day>24</Day></DateCreated><DateCompleted><Year>1998</Year><Month>03</Month><Day>24</Day></DateCompleted><DateRevised><Year>2000</Year><Month>12</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1503</ISSN><JournalIssue CitedMedium="Print"><Volume>89</Volume><Issue>1</Issue><PubDate><MedlineDate>1998 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>The Journal of heredity</Title><ISOAbbreviation>J. Hered.</ISOAbbreviation></Journal><ArticleTitle>The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding.</ArticleTitle><Pagination><MedlinePgn>83-6</MedlinePgn></Pagination><Abstract><AbstractText>In situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin was used to locate rDNA sites on root-tip metaphase chromosomes of Citrus sinensis L. (2n = 2x = 18), Poncirus trifoliata L. Raf. (2n = 2x = 18), and Citrus x Poncirus hybrids (2n = 2x = 18). Counterstaining with the fluorochromes chromomycin A3 and DAPI uniquely identified many but not all chromosomes. C. sinensis had five 18S-25S rDNA sites, P. trifoliata had seven, and three different Citrus x Poncirus hybrids had five or six sites. Four 5S rDNA sites were detected, mostly linked to 18S-25S rDNA sites. 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Schwarzacher</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Roose, M L" sort="Roose, M L" uniqKey="Roose M" first="M L" last="Roose">M L Roose</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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