Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus
Identifieur interne : 001B00 ( Ncbi/Merge ); précédent : 001A99; suivant : 001B01Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus
Auteurs : José Cuenca ; Pablo Aleza ; José Juárez ; Andrés García-Lor ; Yann Froelicher ; Luis Navarro ; Patrick OllitraultSource :
- Scientific Reports [ 2045-2322 ] ; 2015.
Abstract
Polyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (FDR) or Second-Division Restitution (SDR), greatly impacts the gametic and population structures and, therefore, the breeding efficiency. Previous methods to identify the underlying mechanism required the analysis of a large set of markers over large progeny. This work develops a new maximum-likelihood method to identify the unreduced gamete formation mechanism both at the population and individual levels using independent centromeric markers. Knowledge of marker-centromere distances greatly improves the statistical power of the comparison between the SDR and FDR hypotheses. Simulating data demonstrated the importance of selecting markers very close to the centromere to obtain significant conclusions at individual level. This new method was used to identify the meiotic restitution mechanism in nineteen mandarin genotypes used as female parents in triploid citrus breeding. SDR was identified for 85.3% of 543 triploid hybrids and FDR for 0.6%. No significant conclusions were obtained for 14.1% of the hybrids. At population level SDR was the predominant mechanisms for the 19 parental mandarins.
Url:
DOI: 10.1038/srep09897
PubMed: 25894579
PubMed Central: 4403285
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus</title><author><name sortKey="Cuenca, Jose" sort="Cuenca, Jose" uniqKey="Cuenca J" first="José" last="Cuenca">José Cuenca</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Garcia Lor, Andres" sort="Garcia Lor, Andres" uniqKey="Garcia Lor A" first="Andrés" last="García-Lor">Andrés García-Lor</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Froelicher, Yann" sort="Froelicher, Yann" uniqKey="Froelicher Y" first="Yann" last="Froelicher">Yann Froelicher</name><affiliation><nlm:aff id="a2"><institution>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)</institution></nlm:aff></affiliation></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation><affiliation><nlm:aff id="a2"><institution>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)</institution></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25894579</idno><idno type="pmc">4403285</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403285</idno><idno type="RBID">PMC:4403285</idno><idno type="doi">10.1038/srep09897</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000446</idno><idno type="wicri:Area/Pmc/Curation">000445</idno><idno type="wicri:Area/Pmc/Checkpoint">000432</idno><idno type="wicri:Area/Ncbi/Merge">001B00</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus</title><author><name sortKey="Cuenca, Jose" sort="Cuenca, Jose" uniqKey="Cuenca J" first="José" last="Cuenca">José Cuenca</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Garcia Lor, Andres" sort="Garcia Lor, Andres" uniqKey="Garcia Lor A" first="Andrés" last="García-Lor">Andrés García-Lor</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Froelicher, Yann" sort="Froelicher, Yann" uniqKey="Froelicher Y" first="Yann" last="Froelicher">Yann Froelicher</name><affiliation><nlm:aff id="a2"><institution>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)</institution></nlm:aff></affiliation></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation><nlm:aff id="a1"><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></nlm:aff></affiliation><affiliation><nlm:aff id="a2"><institution>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)</institution></nlm:aff></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Polyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (FDR) or Second-Division Restitution (SDR), greatly impacts the gametic and population structures and, therefore, the breeding efficiency. Previous methods to identify the underlying mechanism required the analysis of a large set of markers over large progeny. This work develops a new maximum-likelihood method to identify the unreduced gamete formation mechanism both at the population and individual levels using independent centromeric markers. Knowledge of marker-centromere distances greatly improves the statistical power of the comparison between the SDR and FDR hypotheses. Simulating data demonstrated the importance of selecting markers very close to the centromere to obtain significant conclusions at individual level. This new method was used to identify the meiotic restitution mechanism in nineteen mandarin genotypes used as female parents in triploid citrus breeding. SDR was identified for 85.3% of 543 triploid hybrids and FDR for 0.6%. No significant conclusions were obtained for 14.1% of the hybrids. At population level SDR was the predominant mechanisms for the 19 parental mandarins.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Otto, S P" uniqKey="Otto S">S. P. Otto</name></author><author><name sortKey="Whitton, J" uniqKey="Whitton J">J. Whitton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Soltis, P S" uniqKey="Soltis P">P. S. Soltis</name></author><author><name sortKey="Soltis, D E" uniqKey="Soltis D">D. E. Soltis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harlan, J R" uniqKey="Harlan J">J. R. Harlan</name></author><author><name sortKey="Dewet, J" uniqKey="Dewet J">J. DeWet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Storme, N" uniqKey="De Storme N">N. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Sci Rep</journal-id><journal-id journal-id-type="iso-abbrev">Sci Rep</journal-id><journal-title-group><journal-title>Scientific Reports</journal-title></journal-title-group><issn pub-type="epub">2045-2322</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25894579</article-id><article-id pub-id-type="pmc">4403285</article-id><article-id pub-id-type="pii">srep09897</article-id><article-id pub-id-type="doi">10.1038/srep09897</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Cuenca</surname><given-names>José</given-names></name><xref ref-type="aff" rid="a1">1</xref><xref ref-type="author-notes" rid="n1">*</xref></contrib><contrib contrib-type="author"><name><surname>Aleza</surname><given-names>Pablo</given-names></name><xref ref-type="aff" rid="a1">1</xref><xref ref-type="author-notes" rid="n1">*</xref></contrib><contrib contrib-type="author"><name><surname>Juárez</surname><given-names>José</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>García-Lor</surname><given-names>Andrés</given-names></name><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Froelicher</surname><given-names>Yann</given-names></name><xref ref-type="aff" rid="a2">2</xref></contrib><contrib contrib-type="author"><name><surname>Navarro</surname><given-names>Luis</given-names></name><xref ref-type="corresp" rid="c1">a</xref><xref ref-type="aff" rid="a1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ollitrault</surname><given-names>Patrick</given-names></name><xref ref-type="corresp" rid="c2">b</xref><xref ref-type="aff" rid="a1">1</xref><xref ref-type="aff" rid="a2">2</xref></contrib><aff id="a1"><label>1</label><institution>Crop Protection and Biotechnology Center. Instituto Valenciano de Investigaciones Agrarias (IVIA)</institution></aff><aff id="a2"><label>2</label><institution>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)</institution></aff></contrib-group><author-notes><corresp id="c1"><label>a</label><email>ollitrault@cirad.fr</email></corresp><corresp id="c2"><label>b</label><email>lnavarro@ivia.es</email></corresp><fn id="n1"><label>*</label><p>These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="epub"><day>20</day><month>04</month><year>2015</year></pub-date><pub-date pub-type="collection"><year>2015</year></pub-date><volume>5</volume><elocation-id>9897</elocation-id><history><date date-type="received"><day>30</day><month>11</month><year>2014</year></date><date date-type="accepted"><day>13</day><month>03</month><year>2015</year></date></history><permissions><copyright-statement>Copyright © 2015, Macmillan Publishers Limited. All rights reserved</copyright-statement><copyright-year>2015</copyright-year><copyright-holder>Macmillan Publishers Limited. All rights reserved</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/"><pmc-comment>author-paid</pmc-comment>
<license-p>This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><abstract><p>Polyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (FDR) or Second-Division Restitution (SDR), greatly impacts the gametic and population structures and, therefore, the breeding efficiency. Previous methods to identify the underlying mechanism required the analysis of a large set of markers over large progeny. This work develops a new maximum-likelihood method to identify the unreduced gamete formation mechanism both at the population and individual levels using independent centromeric markers. Knowledge of marker-centromere distances greatly improves the statistical power of the comparison between the SDR and FDR hypotheses. Simulating data demonstrated the importance of selecting markers very close to the centromere to obtain significant conclusions at individual level. This new method was used to identify the meiotic restitution mechanism in nineteen mandarin genotypes used as female parents in triploid citrus breeding. SDR was identified for 85.3% of 543 triploid hybrids and FDR for 0.6%. No significant conclusions were obtained for 14.1% of the hybrids. At population level SDR was the predominant mechanisms for the 19 parental mandarins.</p></abstract></article-meta></front><floats-group><fig id="f1"><label>Figure 1</label><caption><title>Half tetrads resulting from no crossover and single crossover events under FDR and SDR mechanisms of unreduced gamete formation.</title></caption><graphic xlink:href="srep09897-f1"></graphic></fig><fig id="f2"><label>Figure 2</label><caption><title>Rate (percentage) of heterozygosity restitution in the unreduced gametes under FDR and SDR mechanisms in function of the locus-centromere distance considering the total interference model, the no interference model and the Cx(Co)<xref ref-type="bibr" rid="b4">4</xref> partial interference model (adapted from Cuenca <italic>et</italic> <italic>al</italic>.<xref ref-type="bibr" rid="b37">37</xref>).</title></caption><graphic xlink:href="srep09897-f2"></graphic></fig><fig id="f3"><label>Figure 3</label><caption><title>Percentage of replicates with significant LOD value considering a LOD3 for (a) theoretical SDR and (b) FDR populations, and considering a LOD2 for (c) SDR and (d) FDR populations.</title></caption><graphic xlink:href="srep09897-f3"></graphic></fig><fig id="f4"><label>Figure 4</label><caption><title>Number of hybrids needed to obtain significant conclusions for (a) SDR and (b) FDR mechanisms.</title></caption><graphic xlink:href="srep09897-f4"></graphic></fig><fig id="f5"><label>Figure 5</label><caption><title>Frequency histogram of LOD values obtained for each individual 2<italic>n</italic> gamete, indicating those arising from clementines, ‘Fortune’ mandarin and other mandarins analysed in this study.</title></caption><graphic xlink:href="srep09897-f5"></graphic></fig><table-wrap position="float" id="t1"><label>Table 1</label><caption><title>LOD scores for progeny of 19 female parents analysed at population level and individuals within each population originated by SDR, FDR, or with unidentified origin</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th rowspan="2" align="left" valign="top" charoff="50">Group</th><th rowspan="2" align="center" valign="top" charoff="50">Female parent</th><th rowspan="2" align="center" valign="top" charoff="50">Nh</th><th rowspan="2" align="center" valign="top" charoff="50">Nm</th><th rowspan="2" align="center" valign="top" charoff="50">Cd average (cM)</th><th colspan="2" align="center" valign="top" charoff="50">LODs> +3</th><th colspan="2" align="center" valign="top" charoff="50">LODs −3 − +3</th><th colspan="2" align="center" valign="top" charoff="50">LODs <−3</th><th rowspan="2" align="center" valign="top" charoff="50">Population LOD P<sub>SDR</sub>/P<sub>FDR</sub></th></tr><tr><th align="center" valign="top" charoff="50">number</th><th align="center" valign="top" charoff="50">(%)</th><th align="center" valign="top" charoff="50">number</th><th align="center" valign="top" charoff="50">(%)</th><th align="center" valign="top" charoff="50">number</th><th align="center" valign="top" charoff="50">(%)</th></tr></thead><tbody valign="top"><tr><td rowspan="6" align="left" valign="top" charoff="50">Clementine</td><td align="center" valign="top" charoff="50">‘Bruno’</td><td align="char" valign="top" char="." charoff="50">17</td><td align="center" valign="top" charoff="50">6</td><td align="char" valign="top" char="." charoff="50">7.8</td><td align="char" valign="top" char="." charoff="50">15</td><td align="char" valign="top" char="." charoff="50">(88.2)</td><td align="char" valign="top" char="." charoff="50">2</td><td align="char" valign="top" char="." charoff="50">(11.8)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">98.9</td></tr><tr><td align="center" valign="top" charoff="50">‘Clemenules’</td><td align="char" valign="top" char="." charoff="50">23</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">4.4</td><td align="char" valign="top" char="." charoff="50">22</td><td align="char" valign="top" char="." charoff="50">(95.6)</td><td align="char" valign="top" char="." charoff="50">1</td><td align="char" valign="top" char="." charoff="50">(4.4)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">143.3</td></tr><tr><td align="center" valign="top" charoff="50">‘Fina’</td><td align="char" valign="top" char="." charoff="50">87</td><td align="center" valign="top" charoff="50">6</td><td align="char" valign="top" char="." charoff="50">3.7</td><td align="char" valign="top" char="." charoff="50">83</td><td align="char" valign="top" char="." charoff="50">(95.4)</td><td align="char" valign="top" char="." charoff="50">4</td><td align="char" valign="top" char="." charoff="50">(4.6)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">699.3</td></tr><tr><td align="center" valign="top" charoff="50">‘Guillermina’</td><td align="char" valign="top" char="." charoff="50">14</td><td align="center" valign="top" charoff="50">6</td><td align="char" valign="top" char="." charoff="50">8.1</td><td align="char" valign="top" char="." charoff="50">14</td><td align="char" valign="top" char="." charoff="50">(100.0)</td><td align="char" valign="top" char="." charoff="50">0</td><td align="char" valign="top" char="." charoff="50">(0.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">91.1</td></tr><tr><td align="center" valign="top" charoff="50">‘Hernandina’</td><td align="char" valign="top" char="." charoff="50">22</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">4.4</td><td align="char" valign="top" char="." charoff="50">20</td><td align="char" valign="top" char="." charoff="50">(90.9)</td><td align="char" valign="top" char="." charoff="50">2</td><td align="char" valign="top" char="." charoff="50">(9.1)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">139.0</td></tr><tr><td align="center" valign="top" charoff="50">‘Loretina’</td><td align="char" valign="top" char="." charoff="50">2</td><td align="center" valign="top" charoff="50">7</td><td align="char" valign="top" char="." charoff="50">9.5</td><td align="char" valign="top" char="." charoff="50">2</td><td align="char" valign="top" char="." charoff="50">(100.0)</td><td align="char" valign="top" char="." charoff="50">0</td><td align="char" valign="top" char="." charoff="50">(0.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">10.3</td></tr><tr><td align="left" valign="top" charoff="50">Mandarin</td><td align="center" valign="top" charoff="50">‘Imperial’</td><td align="char" valign="top" char="." charoff="50">24</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">5.0</td><td align="char" valign="top" char="." charoff="50">23</td><td align="char" valign="top" char="." charoff="50">(95.8)</td><td align="char" valign="top" char="." charoff="50">1</td><td align="char" valign="top" char="." charoff="50">(4.2)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">138.5</td></tr><tr><td rowspan="18" align="left" valign="top" charoff="50">Hybrid mandarin</td><td align="center" valign="top" charoff="50">‘Ellendale’</td><td align="char" valign="top" char="." charoff="50">69</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">9.1</td><td align="char" valign="top" char="." charoff="50">50</td><td align="char" valign="top" char="." charoff="50">(72.5)</td><td align="char" valign="top" char="." charoff="50">18</td><td align="char" valign="top" char="." charoff="50">(26.1)</td><td align="center" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">(1.4)</td><td align="char" valign="top" char="." charoff="50">282.7</td></tr><tr><td align="center" valign="top" charoff="50">‘Encore’</td><td align="char" valign="top" char="." charoff="50">3</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">4.9</td><td align="char" valign="top" char="." charoff="50">3</td><td align="char" valign="top" char="." charoff="50">(100.0)</td><td align="char" valign="top" char="." charoff="50">0</td><td align="char" valign="top" char="." charoff="50">(0.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">17.9</td></tr><tr><td align="center" valign="top" charoff="50">‘Fallglo’</td><td align="char" valign="top" char="." charoff="50">3</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">3.7</td><td align="char" valign="top" char="." charoff="50">3</td><td align="char" valign="top" char="." charoff="50">(100.0)</td><td align="char" valign="top" char="." charoff="50">0</td><td align="char" valign="top" char="." charoff="50">(0.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">21.6</td></tr><tr><td align="center" valign="top" charoff="50">‘Fortune’</td><td align="char" valign="top" char="." charoff="50">197</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">6.8</td><td align="char" valign="top" char="." charoff="50">162</td><td align="char" valign="top" char="." charoff="50">(82.2)</td><td align="char" valign="top" char="." charoff="50">33</td><td align="char" valign="top" char="." charoff="50">(16.7)</td><td align="center" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">(1.1)</td><td align="char" valign="top" char="." charoff="50">933.0</td></tr><tr><td align="center" valign="top" charoff="50"><italic>‘Fortune’</italic><italic>×</italic><italic>‘Ellendale’</italic></td><td align="char" valign="top" char="." charoff="50"><italic>58</italic></td><td align="center" valign="top" charoff="50"><italic>5</italic></td><td align="char" valign="top" char="." charoff="50"><italic>6.8</italic></td><td align="char" valign="top" char="." charoff="50"><italic>54</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(93.1)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>4</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(6.9)</italic></td><td align="center" valign="top" charoff="50"><italic>0</italic></td><td align="center" valign="top" charoff="50"><italic>(0.0)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>310.5</italic></td></tr><tr><td align="center" valign="top" charoff="50"><italic>‘Fortune’</italic><italic>×</italic><italic>‘Minneola’</italic></td><td align="char" valign="top" char="." charoff="50"><italic>35</italic></td><td align="center" valign="top" charoff="50"><italic>4</italic></td><td align="char" valign="top" char="." charoff="50"><italic>5.2</italic></td><td align="char" valign="top" char="." charoff="50"><italic>28</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(80.0)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>6</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(17.1)</italic></td><td align="center" valign="top" charoff="50"><italic>1</italic></td><td align="center" valign="top" charoff="50"><italic>(2.9)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>145.7</italic></td></tr><tr><td align="center" valign="top" charoff="50"><italic>‘Fortune’</italic><italic>×</italic><italic>‘Murcott’</italic></td><td align="char" valign="top" char="." charoff="50"><italic>67</italic></td><td align="center" valign="top" charoff="50"><italic>5</italic></td><td align="char" valign="top" char="." charoff="50"><italic>6.8</italic></td><td align="char" valign="top" char="." charoff="50"><italic>53</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(79.1)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>14</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(20.9)</italic></td><td align="center" valign="top" charoff="50"><italic>0</italic></td><td align="center" valign="top" charoff="50"><italic>(0.0)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>326.2</italic></td></tr><tr><td align="center" valign="top" charoff="50"><italic>‘Fortune’</italic><italic>×</italic><italic>‘Willowleaf’</italic></td><td align="char" valign="top" char="." charoff="50"><italic>37</italic></td><td align="center" valign="top" charoff="50"><italic>4</italic></td><td align="char" valign="top" char="." charoff="50"><italic>6.6</italic></td><td align="char" valign="top" char="." charoff="50"><italic>28</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(75.7)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>8</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(21.6)</italic></td><td align="center" valign="top" charoff="50"><italic>1</italic></td><td align="center" valign="top" charoff="50"><italic>(2.7)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>150.6</italic></td></tr><tr><td align="center" valign="top" charoff="50">‘Honey’</td><td align="char" valign="top" char="." charoff="50">1</td><td align="center" valign="top" charoff="50">4</td><td align="char" valign="top" char="." charoff="50">6.1</td><td align="char" valign="top" char="." charoff="50">1</td><td align="char" valign="top" char="." charoff="50">(100.0)</td><td align="char" valign="top" char="." charoff="50">0</td><td align="char" valign="top" char="." charoff="50">(0.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">5.1</td></tr><tr><td align="center" valign="top" charoff="50">‘Kiyomi’</td><td align="char" valign="top" char="." charoff="50">21</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">6.3</td><td align="char" valign="top" char="." charoff="50">20</td><td align="char" valign="top" char="." charoff="50">(95.2)</td><td align="char" valign="top" char="." charoff="50">1</td><td align="char" valign="top" char="." charoff="50">(4.8)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">162.9</td></tr><tr><td align="center" valign="top" charoff="50">‘Moncada’</td><td align="char" valign="top" char="." charoff="50">8</td><td align="center" valign="top" charoff="50">4</td><td align="char" valign="top" char="." charoff="50">10.3</td><td align="char" valign="top" char="." charoff="50">4</td><td align="char" valign="top" char="." charoff="50">(50.0)</td><td align="char" valign="top" char="." charoff="50">4</td><td align="char" valign="top" char="." charoff="50">(50.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">22.1</td></tr><tr><td align="center" valign="top" charoff="50">‘Nadorcott’</td><td align="char" valign="top" char="." charoff="50">11</td><td align="center" valign="top" charoff="50">4</td><td align="char" valign="top" char="." charoff="50">8.9</td><td align="char" valign="top" char="." charoff="50">6</td><td align="char" valign="top" char="." charoff="50">(54.5)</td><td align="char" valign="top" char="." charoff="50">5</td><td align="char" valign="top" char="." charoff="50">(45.5)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">23.9</td></tr><tr><td align="center" valign="top" charoff="50">‘Orri’</td><td align="char" valign="top" char="." charoff="50">29</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">10.2</td><td align="char" valign="top" char="." charoff="50">17</td><td align="char" valign="top" char="." charoff="50">(58.6)</td><td align="char" valign="top" char="." charoff="50">12</td><td align="char" valign="top" char="." charoff="50">(41.4)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">84.5</td></tr><tr><td align="center" valign="top" charoff="50"><italic>‘Orri’</italic><italic>×</italic><italic>‘Fortune’</italic></td><td align="char" valign="top" char="." charoff="50"><italic>17</italic></td><td align="center" valign="top" charoff="50"><italic>5</italic></td><td align="char" valign="top" char="." charoff="50"><italic>10.2</italic></td><td align="char" valign="top" char="." charoff="50"><italic>14</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(82.3)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>3</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(17.7)</italic></td><td align="center" valign="top" charoff="50"><italic>0</italic></td><td align="center" valign="top" charoff="50"><italic>(0.0)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>67.5</italic></td></tr><tr><td align="center" valign="top" charoff="50"><italic>‘Orri’</italic><italic>×</italic><italic>‘Oronules’</italic></td><td align="char" valign="top" char="." charoff="50"><italic>12</italic></td><td align="center" valign="top" charoff="50"><italic>5</italic></td><td align="char" valign="top" char="." charoff="50"><italic>10.2</italic></td><td align="char" valign="top" char="." charoff="50"><italic>3</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(25.0)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>9</italic></td><td align="char" valign="top" char="." charoff="50"><italic>(75.0)</italic></td><td align="center" valign="top" charoff="50"><italic>0</italic></td><td align="center" valign="top" charoff="50"><italic>(0.0)</italic></td><td align="char" valign="top" char="." charoff="50"><italic>17.0</italic></td></tr><tr><td align="center" valign="top" charoff="50">‘Ortanique’</td><td align="char" valign="top" char="." charoff="50">6</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">6.6</td><td align="char" valign="top" char="." charoff="50">6</td><td align="char" valign="top" char="." charoff="50">(100.0)</td><td align="char" valign="top" char="." charoff="50">0</td><td align="char" valign="top" char="." charoff="50">(0.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">28.1</td></tr><tr><td align="center" valign="top" charoff="50">‘Umatilla’</td><td align="char" valign="top" char="." charoff="50">5</td><td align="center" valign="top" charoff="50">4</td><td align="char" valign="top" char="." charoff="50">11.3</td><td align="char" valign="top" char="." charoff="50">1</td><td align="char" valign="top" char="." charoff="50">(20.0)</td><td align="char" valign="top" char="." charoff="50">4</td><td align="char" valign="top" char="." charoff="50">(80.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">9.6</td></tr><tr><td align="center" valign="top" charoff="50">‘Wilking’</td><td align="char" valign="top" char="." charoff="50">1</td><td align="center" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">8.3</td><td align="char" valign="top" char="." charoff="50">1</td><td align="char" valign="top" char="." charoff="50">(100.0)</td><td align="char" valign="top" char="." charoff="50">0</td><td align="char" valign="top" char="." charoff="50">(0.0)</td><td align="center" valign="top" charoff="50">0</td><td align="center" valign="top" charoff="50">(0.0)</td><td align="char" valign="top" char="." charoff="50">15.6</td></tr></tbody></table><table-wrap-foot><fn id="t1-fn1"><p>Nh: number of hybrids within each population (pop). Nm: number of markers analyzed over each population. Cd: Centromere distance. Italic format indicates different populations derived from ‘Fortune’ and ‘Orri’ female parents</p></fn></table-wrap-foot></table-wrap><table-wrap position="float" id="t2"><label>Table 2</label><caption><title>Number of hybrids within each population analysed in this study</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50"># population</th><th align="center" valign="top" charoff="50">Population</th><th align="center" valign="top" charoff="50">Number of hybrids</th><th align="left" valign="top" charoff="50"> </th><th align="center" valign="top" charoff="50"># population</th><th align="center" valign="top" charoff="50">Population</th><th align="center" valign="top" charoff="50">Number of hybrids</th></tr></thead><tbody valign="top"><tr><td align="left" valign="top" charoff="50">1</td><td align="center" valign="top" charoff="50">Bruno × Chandler</td><td align="char" valign="top" char="." charoff="50">17</td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">10</td><td align="center" valign="top" charoff="50">Honey × N’15</td><td align="char" valign="top" char="." charoff="50">1</td></tr><tr><td align="left" valign="top" charoff="50">2</td><td align="center" valign="top" charoff="50">Clemenules × Nadorcott</td><td align="char" valign="top" char="." charoff="50">23</td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">11</td><td align="center" valign="top" charoff="50">Imperial × Moncada</td><td align="char" valign="top" char="." charoff="50">24</td></tr><tr><td align="left" valign="top" charoff="50">3</td><td align="center" valign="top" charoff="50">Ellendale × Fortune</td><td align="char" valign="top" char="." charoff="50">69</td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">12</td><td align="center" valign="top" charoff="50">Kiyomi × Nadorcott</td><td align="char" valign="top" char="." charoff="50">21</td></tr><tr><td align="left" valign="top" charoff="50">4</td><td align="center" valign="top" charoff="50">Encore × Ellendale</td><td align="char" valign="top" char="." charoff="50">3</td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">13</td><td align="center" valign="top" charoff="50">Loretina × Chandler</td><td align="char" valign="top" char="." charoff="50">2</td></tr><tr><td align="left" valign="top" charoff="50">5</td><td align="center" valign="top" charoff="50">Fallglo × N’15</td><td align="char" valign="top" char="." charoff="50">3</td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">14</td><td align="center" valign="top" charoff="50">Moncada × Ellendale</td><td align="char" valign="top" char="." charoff="50">8</td></tr><tr><td align="left" valign="top" charoff="50">6</td><td align="center" valign="top" charoff="50">Fina × Nadorcott</td><td align="char" valign="top" char="." charoff="50">87</td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">15</td><td align="center" valign="top" charoff="50">Nadorcott × Ellendale</td><td align="char" valign="top" char="." charoff="50">11</td></tr><tr><td align="left" valign="top" charoff="50">7</td><td align="center" valign="top" charoff="50">Fortune × 4 male parents</td><td align="char" valign="top" char="." charoff="50">197</td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">16</td><td align="center" valign="top" charoff="50">Orri × 2 male parents</td><td align="char" valign="top" char="." charoff="50">29</td></tr><tr><td align="left" valign="top" charoff="50"><italic>7a</italic></td><td align="center" valign="top" charoff="50"><italic>Fortune</italic> × <italic>Ellendale</italic></td><td align="char" valign="top" char="." charoff="50"><italic>58</italic></td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50"><italic>16a</italic></td><td align="center" valign="top" charoff="50"><italic>Orri</italic> × <italic>Fortune</italic></td><td align="char" valign="top" char="." charoff="50"><italic>17</italic></td></tr><tr><td align="left" valign="top" charoff="50"><italic>7b</italic></td><td align="center" valign="top" charoff="50"><italic>Fortune</italic> × <italic>Minneola</italic></td><td align="char" valign="top" char="." charoff="50"><italic>35</italic></td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50"><italic>16b</italic></td><td align="center" valign="top" charoff="50"><italic>Orri</italic> × <italic>Oronules</italic></td><td align="char" valign="top" char="." charoff="50"><italic>12</italic></td></tr><tr><td align="left" valign="top" charoff="50"><italic>7c</italic></td><td align="center" valign="top" charoff="50"><italic>Fortune</italic> × <italic>Murcott</italic></td><td align="char" valign="top" char="." charoff="50"><italic>67</italic></td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">17</td><td align="center" valign="top" charoff="50">Ortanique × Wilking</td><td align="char" valign="top" char="." charoff="50">6</td></tr><tr><td align="left" valign="top" charoff="50"><italic>7d</italic></td><td align="center" valign="top" charoff="50"><italic>Fortune</italic> × <italic>Willowleaf</italic></td><td align="char" valign="top" char="." charoff="50"><italic>37</italic></td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">18</td><td align="center" valign="top" charoff="50">Umatilla × Simeto</td><td align="char" valign="top" char="." charoff="50">5</td></tr><tr><td align="left" valign="top" charoff="50">8</td><td align="center" valign="top" charoff="50">Guillermina × Chandler</td><td align="char" valign="top" char="." charoff="50">14</td><td align="left" valign="top" charoff="50"> </td><td align="char" valign="top" char="." charoff="50">19</td><td align="center" valign="top" charoff="50">Wilking × Fina</td><td align="char" valign="top" char="." charoff="50">1</td></tr><tr><td align="left" valign="top" charoff="50">9</td><td align="center" valign="top" charoff="50">Hernandina × Nadorcott</td><td align="char" valign="top" char="." charoff="50">22</td><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td></tr></tbody></table></table-wrap><table-wrap position="float" id="t3"><label>Table 3</label><caption><title>Centromeric markers used for genotyping each triploid population</title></caption><table frame="hsides" rules="groups" border="1"><colgroup><col align="left"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col><col align="center"></col></colgroup><thead valign="bottom"><tr><th align="left" valign="top" charoff="50">LG</th><th align="center" valign="top" charoff="50">Centromere Position (cM)</th><th align="center" valign="top" charoff="50">Marker id</th><th align="center" valign="top" charoff="50">Marker type</th><th align="center" valign="top" charoff="50">Reference</th><th align="center" valign="top" charoff="50">Marker Position (cM)</th><th align="center" valign="top" charoff="50">Centomere-distance (cM)</th><th align="center" valign="top" charoff="50">Populations analyzed (#)</th></tr></thead><tbody valign="top"><tr><td align="left" valign="top" charoff="50">1</td><td align="char" valign="top" char="." charoff="50">60.66</td><td align="center" valign="top" charoff="50">mCrCIR06B05</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">73</td><td align="char" valign="top" char="." charoff="50">50.27</td><td align="char" valign="top" char="." charoff="50">10.39</td><td align="center" valign="top" charoff="50">7</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CID0806</td><td align="center" valign="top" charoff="50">InDel</td><td align="center" valign="top" charoff="50">74</td><td align="char" valign="top" char="." charoff="50">55.17</td><td align="char" valign="top" char="." charoff="50">5.49</td><td align="center" valign="top" charoff="50">8</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CIBE5720</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">75</td><td align="char" valign="top" char="." charoff="50">58.45</td><td align="char" valign="top" char="." charoff="50">2.21</td><td align="center" valign="top" charoff="50">4,5,11,12,16,17</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">MEST539</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">In preparation</td><td align="char" valign="top" char="." charoff="50">61.82</td><td align="char" valign="top" char="." charoff="50">1.16</td><td align="center" valign="top" charoff="50">6</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">MEST001</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">38</td><td align="char" valign="top" char="." charoff="50">70.60</td><td align="char" valign="top" char="." charoff="50">9.94</td><td align="center" valign="top" charoff="50">10,18,19</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">mCrCIR07D05</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">37</td><td align="char" valign="top" char="." charoff="50">75.60</td><td align="char" valign="top" char="." charoff="50">14.94</td><td align="center" valign="top" charoff="50">1,13,14,17,18</td></tr><tr><td align="left" valign="top" charoff="50">2</td><td align="char" valign="top" char="." charoff="50">56.87</td><td align="center" valign="top" charoff="50">CX2004</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">38</td><td align="char" valign="top" char="." charoff="50">46.67</td><td align="char" valign="top" char="." charoff="50">10.20</td><td align="center" valign="top" charoff="50">18</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CX6F23</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">38</td><td align="char" valign="top" char="." charoff="50">49.53</td><td align="char" valign="top" char="." charoff="50">7.34</td><td align="center" valign="top" charoff="50">1,2,4,5,6,7,9,10,11,12,13,15,16,17,19</td></tr><tr><td align="left" valign="top" charoff="50">3</td><td align="char" valign="top" char="." charoff="50">90.59</td><td align="center" valign="top" charoff="50">CIBE4225</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">75</td><td align="char" valign="top" char="." charoff="50">86.33</td><td align="char" valign="top" char="." charoff="50">4.26</td><td align="center" valign="top" charoff="50">4,12</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CID5376</td><td align="center" valign="top" charoff="50">InDel</td><td align="center" valign="top" charoff="50">74</td><td align="char" valign="top" char="." charoff="50">88.24</td><td align="char" valign="top" char="." charoff="50">2.35</td><td align="center" valign="top" charoff="50">17</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">MEST470</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">In preparation</td><td align="char" valign="top" char="." charoff="50">88.76</td><td align="char" valign="top" char="." charoff="50">1.83</td><td align="center" valign="top" charoff="50">6</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CX0124</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">In preparation</td><td align="char" valign="top" char="." charoff="50">110.28</td><td align="char" valign="top" char="." charoff="50">19.69</td><td align="center" valign="top" charoff="50">13,14,16</td></tr><tr><td align="left" valign="top" charoff="50">4</td><td align="char" valign="top" char="." charoff="50">16.14</td><td align="center" valign="top" charoff="50">mCrCIR07D06</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">37</td><td align="char" valign="top" char="." charoff="50">16.33</td><td align="char" valign="top" char="." charoff="50">0.19</td><td align="center" valign="top" charoff="50">1,7,8,13</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CF-ACA01</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">In preparation</td><td align="char" valign="top" char="." charoff="50">24.41</td><td align="char" valign="top" char="." charoff="50">8.27</td><td align="center" valign="top" charoff="50">2,4,6,9,11</td></tr><tr><td align="left" valign="top" charoff="50">5</td><td align="char" valign="top" char="." charoff="50">23.12</td><td align="center" valign="top" charoff="50">CID0245</td><td align="center" valign="top" charoff="50">InDel</td><td align="center" valign="top" charoff="50">In preparation</td><td align="char" valign="top" char="." charoff="50">20.94</td><td align="char" valign="top" char="." charoff="50">2.18</td><td align="center" valign="top" charoff="50">2,5,6,9</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">MEST104</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">76</td><td align="char" valign="top" char="." charoff="50">40.46</td><td align="char" valign="top" char="." charoff="50">17.34</td><td align="center" valign="top" charoff="50">1,3,8,12,13,14,15,16,18,19</td></tr><tr><td align="left" valign="top" charoff="50">6</td><td align="char" valign="top" char="." charoff="50">6.4</td><td align="center" valign="top" charoff="50">MEST191</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">In preparation</td><td align="char" valign="top" char="." charoff="50">10.86</td><td align="char" valign="top" char="." charoff="50">4.46</td><td align="center" valign="top" charoff="50">1,5,8,10,11,12,13,15,16,19</td></tr><tr><td align="left" valign="top" charoff="50">7</td><td align="char" valign="top" char="." charoff="50">96.43</td><td align="center" valign="top" charoff="50">mCrCIR03B07</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">37</td><td align="char" valign="top" char="." charoff="50">83.39</td><td align="char" valign="top" char="." charoff="50">13.04</td><td align="center" valign="top" charoff="50">7</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CX0114</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">In preparation</td><td align="char" valign="top" char="." charoff="50">94.97</td><td align="char" valign="top" char="." charoff="50">1.46</td><td align="center" valign="top" charoff="50">3</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CI07C07</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">73</td><td align="char" valign="top" char="." charoff="50">98.02</td><td align="char" valign="top" char="." charoff="50">1.59</td><td align="center" valign="top" charoff="50">2,3,6,9,10</td></tr><tr><td align="left" valign="top" charoff="50">8</td><td align="char" valign="top" char="." charoff="50">54.21</td><td align="center" valign="top" charoff="50">mCrCIR07B05</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">73</td><td align="char" valign="top" char="." charoff="50">31.70</td><td align="char" valign="top" char="." charoff="50">22.51</td><td align="center" valign="top" charoff="50">3</td></tr><tr><td align="left" valign="top" charoff="50">9</td><td align="char" valign="top" char="." charoff="50">52.16</td><td align="center" valign="top" charoff="50">mCrCIR07F11</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">77</td><td align="char" valign="top" char="." charoff="50">49.57</td><td align="char" valign="top" char="." charoff="50">2.59</td><td align="center" valign="top" charoff="50">1,2,3,4,5,8,9,10,11,13,14,15,17,18,19</td></tr><tr><td align="left" valign="top" charoff="50"> </td><td align="left" valign="top" charoff="50"> </td><td align="center" valign="top" charoff="50">CI08C05</td><td align="center" valign="top" charoff="50">SSR</td><td align="center" valign="top" charoff="50">73</td><td align="char" valign="top" char="." charoff="50">55.14</td><td align="char" valign="top" char="." charoff="50">2.98</td><td align="center" valign="top" charoff="50">7</td></tr></tbody></table></table-wrap></floats-group></pmc><affiliations><list></list><tree><noCountry><name sortKey="Aleza, Pablo" sort="Aleza, Pablo" uniqKey="Aleza P" first="Pablo" last="Aleza">Pablo Aleza</name><name sortKey="Cuenca, Jose" sort="Cuenca, Jose" uniqKey="Cuenca J" first="José" last="Cuenca">José Cuenca</name><name sortKey="Froelicher, Yann" sort="Froelicher, Yann" uniqKey="Froelicher Y" first="Yann" last="Froelicher">Yann Froelicher</name><name sortKey="Garcia Lor, Andres" sort="Garcia Lor, Andres" uniqKey="Garcia Lor A" first="Andrés" last="García-Lor">Andrés García-Lor</name><name sortKey="Juarez, Jose" sort="Juarez, Jose" uniqKey="Juarez J" first="José" last="Juárez">José Juárez</name><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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