UGT74D1 Catalyzes the Glucosylation of 2-Oxindole-3-Acetic Acid in the Auxin Metabolic Pathway in Arabidopsis
Identifieur interne : 001518 ( Ncbi/Merge ); précédent : 001517; suivant : 001519UGT74D1 Catalyzes the Glucosylation of 2-Oxindole-3-Acetic Acid in the Auxin Metabolic Pathway in Arabidopsis
Auteurs : Keita Tanaka [Japon] ; Ken-Ichiro Hayashi [Japon] ; Masahiro Natsume [Japon] ; Yuji Kamiya [Japon] ; Hitoshi Sakakibara [Japon] ; Hiroshi Kawaide [Japon] ; Hiroyuki Kasahara [Japon]Source :
- Plant and Cell Physiology [ 0032-0781 ] ; 2013.
Abstract
IAA is a naturally occurring auxin that plays a crucial role in the regulation of plant
growth and development. The endogenous concentration of IAA is spatiotemporally regulated
by biosynthesis, transport and its inactivation in plants. Previous studies have shown
that the metabolism of IAA to 2-oxindole-3-acetic acid (OxIAA) and OxIAA-glucoside
(OxIAA-Glc) may play an important role in IAA homeostasis, but the genes involved in this
metabolic pathway are still unknown. In this study, we show that UGT74D1 catalyzes the
glucosylation of OxIAA in
Url:
DOI: 10.1093/pcp/pct173
PubMed: 24285754
PubMed Central: 3894777
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PMC:3894777Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">UGT74D1 Catalyzes the Glucosylation of 2-Oxindole-3-Acetic Acid in the Auxin
Metabolic Pathway in <italic>Arabidopsis</italic>
</title>
<author><name sortKey="Tanaka, Keita" sort="Tanaka, Keita" uniqKey="Tanaka K" first="Keita" last="Tanaka">Keita Tanaka</name>
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<author><name sortKey="Hayashi, Ken Ichiro" sort="Hayashi, Ken Ichiro" uniqKey="Hayashi K" first="Ken-Ichiro" last="Hayashi">Ken-Ichiro Hayashi</name>
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<author><name sortKey="Natsume, Masahiro" sort="Natsume, Masahiro" uniqKey="Natsume M" first="Masahiro" last="Natsume">Masahiro Natsume</name>
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<author><name sortKey="Kamiya, Yuji" sort="Kamiya, Yuji" uniqKey="Kamiya Y" first="Yuji" last="Kamiya">Yuji Kamiya</name>
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<author><name sortKey="Sakakibara, Hitoshi" sort="Sakakibara, Hitoshi" uniqKey="Sakakibara H" first="Hitoshi" last="Sakakibara">Hitoshi Sakakibara</name>
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<author><name sortKey="Kawaide, Hiroshi" sort="Kawaide, Hiroshi" uniqKey="Kawaide H" first="Hiroshi" last="Kawaide">Hiroshi Kawaide</name>
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<author><name sortKey="Kasahara, Hiroyuki" sort="Kasahara, Hiroyuki" uniqKey="Kasahara H" first="Hiroyuki" last="Kasahara">Hiroyuki Kasahara</name>
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<series><title level="j">Plant and Cell Physiology</title>
<idno type="ISSN">0032-0781</idno>
<idno type="eISSN">1471-9053</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><p>IAA is a naturally occurring auxin that plays a crucial role in the regulation of plant
growth and development. The endogenous concentration of IAA is spatiotemporally regulated
by biosynthesis, transport and its inactivation in plants. Previous studies have shown
that the metabolism of IAA to 2-oxindole-3-acetic acid (OxIAA) and OxIAA-glucoside
(OxIAA-Glc) may play an important role in IAA homeostasis, but the genes involved in this
metabolic pathway are still unknown. In this study, we show that UGT74D1 catalyzes the
glucosylation of OxIAA in <italic>Arabidopsis</italic>
. By screening yeasts transformed
with <italic>Arabidopsis UDP-glycosyltransferase</italic>
(<italic>UGT</italic>
) genes, we
found that OxIAA-Glc accumulates in the culture media of yeasts expressing
<italic>UGT74D1</italic>
in the presence of OxIAA. Further, we showed that UGT74D1
expressed in <italic>Escherichia coli</italic>
converts OxIAA to OxIAA-Glc. The endogenous
concentration of OxIAA-Glc decreased by 85% while that of OxIAA increased 2.5-fold
in <italic>ugt74d1</italic>
-deficient mutants, indicating the major role of UGT74D1 in
OxIAA metabolism. Moreover, the induction of <italic>UGT74D1</italic>
markedly increased
the level of OxIAA-Glc and loss of root gravitropism. These results indicate that UGT74D1
catalyzes a committed step in the OxIAA-dependent IAA metabolic pathway in
<italic>Arabidopsis</italic>
.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell Physiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Plant Cell Physiol</journal-id>
<journal-id journal-id-type="publisher-id">pcp</journal-id>
<journal-id journal-id-type="hwp">pcellphys</journal-id>
<journal-title-group><journal-title>Plant and Cell Physiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0032-0781</issn>
<issn pub-type="epub">1471-9053</issn>
<publisher><publisher-name>Oxford University Press</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24285754</article-id>
<article-id pub-id-type="pmc">3894777</article-id>
<article-id pub-id-type="doi">10.1093/pcp/pct173</article-id>
<article-id pub-id-type="publisher-id">pct173</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Regular Papers</subject>
</subj-group>
</article-categories>
<title-group><article-title>UGT74D1 Catalyzes the Glucosylation of 2-Oxindole-3-Acetic Acid in the Auxin
Metabolic Pathway in <italic>Arabidopsis</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Tanaka</surname>
<given-names>Keita</given-names>
</name>
<xref ref-type="aff" rid="pct173-AFF1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="pct173-AFF2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hayashi</surname>
<given-names>Ken-ichiro</given-names>
</name>
<xref ref-type="aff" rid="pct173-AFF3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Natsume</surname>
<given-names>Masahiro</given-names>
</name>
<xref ref-type="aff" rid="pct173-AFF1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kamiya</surname>
<given-names>Yuji</given-names>
</name>
<xref ref-type="aff" rid="pct173-AFF2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sakakibara</surname>
<given-names>Hitoshi</given-names>
</name>
<xref ref-type="aff" rid="pct173-AFF2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kawaide</surname>
<given-names>Hiroshi</given-names>
</name>
<xref ref-type="aff" rid="pct173-AFF1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kasahara</surname>
<given-names>Hiroyuki</given-names>
</name>
<xref ref-type="aff" rid="pct173-AFF2"><sup>2</sup>
</xref>
<xref ref-type="aff" rid="pct173-AFF4"><sup>4</sup>
</xref>
<xref ref-type="corresp" rid="pct173-COR1">*</xref>
</contrib>
<aff id="pct173-AFF1"><sup>1</sup>
United Graduate School of Agricultural Science, Tokyo University of Agriculture & Technology, Tokyo, 183-8509 Japan</aff>
<aff id="pct173-AFF2"><sup>2</sup>
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045 Japan</aff>
<aff id="pct173-AFF3"><sup>3</sup>
Department of Biochemistry, Okayama University of Science, Okayama, 700-0005 Japan</aff>
<aff id="pct173-AFF4"><sup>4</sup>
Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO), Saitama, 332-0012 Japan</aff>
</contrib-group>
<author-notes><corresp id="pct173-COR1">*Corresponding author: E-mail,
<email>kasahara@riken.jp</email>
; Fax <fax>+81-45-503-9665</fax>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>1</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="epub"><day>26</day>
<month>11</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>26</day>
<month>11</month>
<year>2013</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>55</volume>
<issue>1</issue>
<fpage>218</fpage>
<lpage>228</lpage>
<history><date date-type="received"><day>17</day>
<month>10</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>19</day>
<month>11</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>© The Author 2013. Published by Oxford University Press on behalf
of Japanese Society of Plant Physiologists.</copyright-statement>
<copyright-year>2013</copyright-year>
<license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>
This is an Open Access article distributed under the terms of the Creative Commons
Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>
),
which permits non-commercial re-use, distribution, and reproduction in any medium,
provided the original work is properly cited. For commercial re-use, please contact
journals.permissions@oup.com</license-p>
</license>
</permissions>
<abstract><p>IAA is a naturally occurring auxin that plays a crucial role in the regulation of plant
growth and development. The endogenous concentration of IAA is spatiotemporally regulated
by biosynthesis, transport and its inactivation in plants. Previous studies have shown
that the metabolism of IAA to 2-oxindole-3-acetic acid (OxIAA) and OxIAA-glucoside
(OxIAA-Glc) may play an important role in IAA homeostasis, but the genes involved in this
metabolic pathway are still unknown. In this study, we show that UGT74D1 catalyzes the
glucosylation of OxIAA in <italic>Arabidopsis</italic>
. By screening yeasts transformed
with <italic>Arabidopsis UDP-glycosyltransferase</italic>
(<italic>UGT</italic>
) genes, we
found that OxIAA-Glc accumulates in the culture media of yeasts expressing
<italic>UGT74D1</italic>
in the presence of OxIAA. Further, we showed that UGT74D1
expressed in <italic>Escherichia coli</italic>
converts OxIAA to OxIAA-Glc. The endogenous
concentration of OxIAA-Glc decreased by 85% while that of OxIAA increased 2.5-fold
in <italic>ugt74d1</italic>
-deficient mutants, indicating the major role of UGT74D1 in
OxIAA metabolism. Moreover, the induction of <italic>UGT74D1</italic>
markedly increased
the level of OxIAA-Glc and loss of root gravitropism. These results indicate that UGT74D1
catalyzes a committed step in the OxIAA-dependent IAA metabolic pathway in
<italic>Arabidopsis</italic>
.</p>
</abstract>
<kwd-group><kwd><italic>Arabidopsis</italic>
</kwd>
<kwd>Auxin</kwd>
<kwd>Glucosyltransferase</kwd>
<kwd>Metabolism</kwd>
<kwd>Plant hormone</kwd>
</kwd-group>
<counts><page-count count="11"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Japon</li>
</country>
<settlement><li>Tokyo</li>
</settlement>
</list>
<tree><country name="Japon"><noRegion><name sortKey="Tanaka, Keita" sort="Tanaka, Keita" uniqKey="Tanaka K" first="Keita" last="Tanaka">Keita Tanaka</name>
</noRegion>
<name sortKey="Hayashi, Ken Ichiro" sort="Hayashi, Ken Ichiro" uniqKey="Hayashi K" first="Ken-Ichiro" last="Hayashi">Ken-Ichiro Hayashi</name>
<name sortKey="Kamiya, Yuji" sort="Kamiya, Yuji" uniqKey="Kamiya Y" first="Yuji" last="Kamiya">Yuji Kamiya</name>
<name sortKey="Kasahara, Hiroyuki" sort="Kasahara, Hiroyuki" uniqKey="Kasahara H" first="Hiroyuki" last="Kasahara">Hiroyuki Kasahara</name>
<name sortKey="Kasahara, Hiroyuki" sort="Kasahara, Hiroyuki" uniqKey="Kasahara H" first="Hiroyuki" last="Kasahara">Hiroyuki Kasahara</name>
<name sortKey="Kawaide, Hiroshi" sort="Kawaide, Hiroshi" uniqKey="Kawaide H" first="Hiroshi" last="Kawaide">Hiroshi Kawaide</name>
<name sortKey="Natsume, Masahiro" sort="Natsume, Masahiro" uniqKey="Natsume M" first="Masahiro" last="Natsume">Masahiro Natsume</name>
<name sortKey="Sakakibara, Hitoshi" sort="Sakakibara, Hitoshi" uniqKey="Sakakibara H" first="Hitoshi" last="Sakakibara">Hitoshi Sakakibara</name>
<name sortKey="Tanaka, Keita" sort="Tanaka, Keita" uniqKey="Tanaka K" first="Keita" last="Tanaka">Keita Tanaka</name>
</country>
</tree>
</affiliations>
</record>
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