Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay.
Identifieur interne : 001456 ( Ncbi/Merge ); précédent : 001455; suivant : 001457Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay.
Auteurs : Miriam Aldeguer [Espagne] ; María L Pez-Andreo ; José A. Gabald N ; Antonio PuyetSource :
- Food chemistry [ 0308-8146 ] ; 2014.
Descripteurs français
- Wicri :
- geographic : Espagne.
English descriptors
- KwdEn :
- Beverages (analysis), Citrus (chemistry), Citrus (genetics), Citrus (metabolism), Citrus sinensis (chemistry), Crosses, Genetic, DNA Copy Number Variations, DNA Probes (analysis), DNA Probes (chemistry), DNA Probes (metabolism), DNA, Intergenic (analysis), DNA, Intergenic (chemistry), DNA, Intergenic (metabolism), DNA, Plant (analysis), DNA, Plant (chemistry), DNA, Plant (metabolism), Fluorescent Dyes (chemistry), Food Contamination, Food Handling, Food Inspection (methods), Food Labeling, Food Quality, Fruit (chemistry), Fruit (genetics), Fruit (metabolism), Limit of Detection, Polymorphism, Single Nucleotide, Real-Time Polymerase Chain Reaction, Rhodamines (chemistry), Spain, Species Specificity.
- MESH :
- chemical , analysis : DNA Probes, DNA, Intergenic, DNA, Plant.
- geographic : Spain.
- analysis : Beverages.
- chemistry : Citrus, Citrus sinensis, DNA Probes, DNA, Intergenic, DNA, Plant, Fluorescent Dyes, Fruit, Rhodamines.
- genetics : Citrus, Fruit.
- metabolism : Citrus, DNA Probes, DNA, Intergenic, DNA, Plant, Fruit.
- methods : Food Inspection.
- Crosses, Genetic, DNA Copy Number Variations, Food Contamination, Food Handling, Food Labeling, Food Quality, Limit of Detection, Polymorphism, Single Nucleotide, Real-Time Polymerase Chain Reaction, Species Specificity.
Abstract
A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin × tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C(T) bias allowed accurate quantitative measurements to be obtained.
DOI: 10.1016/j.foodchem.2013.09.002
PubMed: 24128588
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Gabald N</name></author><author><name sortKey="Puyet, Antonio" sort="Puyet, Antonio" uniqKey="Puyet A" first="Antonio" last="Puyet">Antonio Puyet</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24128588</idno><idno type="pmid">24128588</idno><idno type="doi">10.1016/j.foodchem.2013.09.002</idno><idno type="wicri:Area/PubMed/Corpus">000368</idno><idno type="wicri:Area/PubMed/Curation">000368</idno><idno type="wicri:Area/PubMed/Checkpoint">000368</idno><idno type="wicri:Area/Ncbi/Merge">001456</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay.</title><author><name sortKey="Aldeguer, Miriam" sort="Aldeguer, Miriam" uniqKey="Aldeguer M" first="Miriam" last="Aldeguer">Miriam Aldeguer</name><affiliation wicri:level="1"><nlm:affiliation>Centro Tecnológico Nacional de la Conserva, Molina de Segura, Murcia, Spain; Departamento de Ciencia y Tecnología de Alimentos, Universidad Católica San Antonio de Murcia (UCAM), Murcia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro Tecnológico Nacional de la Conserva, Molina de Segura, Murcia, Spain; Departamento de Ciencia y Tecnología de Alimentos, Universidad Católica San Antonio de Murcia (UCAM), Murcia</wicri:regionArea><wicri:noRegion>Murcia</wicri:noRegion></affiliation></author><author><name sortKey="L Pez Andreo, Maria" sort="L Pez Andreo, Maria" uniqKey="L Pez Andreo M" first="María" last="L Pez-Andreo">María L Pez-Andreo</name></author><author><name sortKey="Gabald N, Jose A" sort="Gabald N, Jose A" uniqKey="Gabald N J" first="José A" last="Gabald N">José A. Gabald N</name></author><author><name sortKey="Puyet, Antonio" sort="Puyet, Antonio" uniqKey="Puyet A" first="Antonio" last="Puyet">Antonio Puyet</name></author></analytic><series><title level="j">Food chemistry</title><idno type="ISSN">0308-8146</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Beverages (analysis)</term><term>Citrus (chemistry)</term><term>Citrus (genetics)</term><term>Citrus (metabolism)</term><term>Citrus sinensis (chemistry)</term><term>Crosses, Genetic</term><term>DNA Copy Number Variations</term><term>DNA Probes (analysis)</term><term>DNA Probes (chemistry)</term><term>DNA Probes (metabolism)</term><term>DNA, Intergenic (analysis)</term><term>DNA, Intergenic (chemistry)</term><term>DNA, Intergenic (metabolism)</term><term>DNA, Plant (analysis)</term><term>DNA, Plant (chemistry)</term><term>DNA, Plant (metabolism)</term><term>Fluorescent Dyes (chemistry)</term><term>Food Contamination</term><term>Food Handling</term><term>Food Inspection (methods)</term><term>Food Labeling</term><term>Food Quality</term><term>Fruit (chemistry)</term><term>Fruit (genetics)</term><term>Fruit (metabolism)</term><term>Limit of Detection</term><term>Polymorphism, Single Nucleotide</term><term>Real-Time Polymerase Chain Reaction</term><term>Rhodamines (chemistry)</term><term>Spain</term><term>Species Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA Probes</term><term>DNA, Intergenic</term><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Spain</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term><term>Citrus sinensis</term><term>DNA Probes</term><term>DNA, Intergenic</term><term>DNA, Plant</term><term>Fluorescent Dyes</term><term>Fruit</term><term>Rhodamines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term><term>Fruit</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus</term><term>DNA Probes</term><term>DNA, Intergenic</term><term>DNA, Plant</term><term>Fruit</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Food Inspection</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crosses, Genetic</term><term>DNA Copy Number Variations</term><term>Food Contamination</term><term>Food Handling</term><term>Food Labeling</term><term>Food Quality</term><term>Limit of Detection</term><term>Polymorphism, Single Nucleotide</term><term>Real-Time Polymerase Chain Reaction</term><term>Species Specificity</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Espagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin × tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C(T) bias allowed accurate quantitative measurements to be obtained.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24128588</PMID><DateCreated><Year>2013</Year><Month>10</Month><Day>16</Day></DateCreated><DateCompleted><Year>2014</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2013</Year><Month>10</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0308-8146</ISSN><JournalIssue CitedMedium="Internet"><Volume>145</Volume><PubDate><Year>2014</Year><Month>Feb</Month><Day>15</Day></PubDate></JournalIssue><Title>Food chemistry</Title><ISOAbbreviation>Food Chem</ISOAbbreviation></Journal><ArticleTitle>Detection of mandarin in orange juice by single-nucleotide polymorphism qPCR assay.</ArticleTitle><Pagination><MedlinePgn>1086-91</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.foodchem.2013.09.002</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0308-8146(13)01242-9</ELocationID><Abstract><AbstractText>A dual-probe real time PCR (qPCR) DNA-based analysis was devised for the identification of mandarin in orange juice. A single nucleotide polymorphism at the trnL-trnF intergenic region of the chloroplast chromosome was confirmed in nine orange (Citrus sinensis) and thirteen commercial varieties of mandarin, including Citrus reticulata and Citrus unshiu species and a mandarin × tangelo hybrid. Two short minor-groove binding fluorescent probes targeting the polymorphic sequence were used in the dual-probe qPCR, which allowed the detection of both species in single-tube reactions. The similarity of PCR efficiencies allowed a simple estimation of the ratio mandarin/orange in the juice samples, which correlated to the measured difference of threshold cycle values for both probes. The limit of detection of the assay was 5% of mandarin in orange juice, both when the juice was freshly prepared (not from concentrate) or reconstituted from concentrate, which would allow the detection of fraudulently added mandarin juice. The possible use of the dual-probe system for quantitative measurements was also tested on fruit juice mixtures. qPCR data obtained from samples containing equal amounts of mandarin and orange juice revealed that the mandarin target copy number was approximately 2.6-fold higher than in orange juice. The use of a matrix-adapted control as calibrator to compensate the resulting C(T) bias allowed accurate quantitative measurements to be obtained.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier Ltd. 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Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012235">Rhodamines</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001628" MajorTopicYN="N">Beverages</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002957" MajorTopicYN="N">Citrus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003433" MajorTopicYN="N">Crosses, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName 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MajorTopicYN="N">Citrus</Keyword><Keyword MajorTopicYN="N">FC</Keyword><Keyword MajorTopicYN="N">Food quality</Keyword><Keyword MajorTopicYN="N">MGB</Keyword><Keyword MajorTopicYN="N">MGB probe</Keyword><Keyword MajorTopicYN="N">NFC</Keyword><Keyword MajorTopicYN="N">Real-time PCR</Keyword><Keyword MajorTopicYN="N">Species identification</Keyword><Keyword MajorTopicYN="N">Tm</Keyword><Keyword MajorTopicYN="N">juice from concentrate</Keyword><Keyword MajorTopicYN="N">juice not from concentrate</Keyword><Keyword MajorTopicYN="N">melting temperature</Keyword><Keyword MajorTopicYN="N">minor groove binding</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>6</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>2</Month><Day>6</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>9</Month><Day>2</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>10</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>6</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24128588</ArticleId><ArticleId IdType="pii">S0308-8146(13)01242-9</ArticleId><ArticleId IdType="doi">10.1016/j.foodchem.2013.09.002</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Gabald N, Jose A" sort="Gabald N, Jose A" uniqKey="Gabald N J" first="José A" last="Gabald N">José A. Gabald N</name><name sortKey="L Pez Andreo, Maria" sort="L Pez Andreo, Maria" uniqKey="L Pez Andreo M" first="María" last="L Pez-Andreo">María L Pez-Andreo</name><name sortKey="Puyet, Antonio" sort="Puyet, Antonio" uniqKey="Puyet A" first="Antonio" last="Puyet">Antonio Puyet</name></noCountry><country name="Espagne"><noRegion><name sortKey="Aldeguer, Miriam" sort="Aldeguer, Miriam" uniqKey="Aldeguer M" first="Miriam" last="Aldeguer">Miriam Aldeguer</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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