N-Acetylcysteine in Agriculture, a Novel Use for an Old Molecule: Focus on Controlling the Plant–Pathogen Xylella fastidiosa
Identifieur interne : 001411 ( Ncbi/Merge ); précédent : 001410; suivant : 001412N-Acetylcysteine in Agriculture, a Novel Use for an Old Molecule: Focus on Controlling the Plant–Pathogen Xylella fastidiosa
Auteurs : Lígia S. Muranaka [Brésil] ; Thais E. Giorgiano [Brésil] ; Marco A. Takita [Brésil] ; Moacir R. Forim [Brésil] ; Luis F. C. Silva [Brésil] ; Helvécio D. Coletta-Filho [Brésil] ; Marcos A. Machado [Brésil] ; Alessandra A. De Souza [Brésil]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Url:
DOI: 10.1371/journal.pone.0072937
PubMed: 24009716
PubMed Central: 3751844
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PMC:3751844Le document en format XML
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<author><name sortKey="De Souza, Alessandra A" sort="De Souza, Alessandra A" uniqKey="De Souza A" first="Alessandra A." last="De Souza">Alessandra A. De Souza</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">N-Acetylcysteine in Agriculture, a Novel Use for an Old Molecule: Focus on Controlling the Plant–Pathogen <italic>Xylella fastidiosa</italic>
</title>
<author><name sortKey="Muranaka, Ligia S" sort="Muranaka, Ligia S" uniqKey="Muranaka L" first="Lígia S." last="Muranaka">Lígia S. Muranaka</name>
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<affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Departamento de Genética e Biologia Molecular, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil</addr-line>
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<author><name sortKey="Takita, Marco A" sort="Takita, Marco A" uniqKey="Takita M" first="Marco A." last="Takita">Marco A. Takita</name>
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<author><name sortKey="Forim, Moacir R" sort="Forim, Moacir R" uniqKey="Forim M" first="Moacir R." last="Forim">Moacir R. Forim</name>
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<author><name sortKey="Silva, Luis F C" sort="Silva, Luis F C" uniqKey="Silva L" first="Luis F. C." last="Silva">Luis F. C. Silva</name>
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<placeName><settlement type="city">São Paulo</settlement>
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<author><name sortKey="Coletta Filho, Helvecio D" sort="Coletta Filho, Helvecio D" uniqKey="Coletta Filho H" first="Helvécio D." last="Coletta-Filho">Helvécio D. Coletta-Filho</name>
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<country xml:lang="fr">Brésil</country>
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<author><name sortKey="Machado, Marcos A" sort="Machado, Marcos A" uniqKey="Machado M" first="Marcos A." last="Machado">Marcos A. Machado</name>
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<country xml:lang="fr">Brésil</country>
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<author><name sortKey="De Souza, Alessandra A" sort="De Souza, Alessandra A" uniqKey="De Souza A" first="Alessandra A." last="De Souza">Alessandra A. De Souza</name>
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</nlm:aff>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Centro de Citricultura Sylvio Moreira, Instituto Agronômico, Cordeirópolis, São Paulo</wicri:regionArea>
<placeName><settlement type="city">São Paulo</settlement>
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<series><title level="j">PLoS ONE</title>
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<front><div type="abstract" xml:lang="en"><p><italic><named-content content-type="genus-species">Xylella fastidiosa</named-content>
</italic>
is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC), a cysteine analogue used mainly to treat human diseases, on <italic><named-content content-type="genus-species">X. fastidiosa</named-content>
</italic>
in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS). The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to <italic><named-content content-type="genus-species">X. fastidiosa</named-content>
</italic>
-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer). HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL) in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria.</p>
</div>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24009716</article-id>
<article-id pub-id-type="pmc">3751844</article-id>
<article-id pub-id-type="publisher-id">PONE-D-13-22328</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0072937</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>N-Acetylcysteine in Agriculture, a Novel Use for an Old Molecule: Focus on Controlling the Plant–Pathogen <italic>Xylella fastidiosa</italic>
</article-title>
<alt-title alt-title-type="running-head">NAC Affects Bacterial Growth and Disease Progress</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Muranaka</surname>
<given-names>Lígia S.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Giorgiano</surname>
<given-names>Thais E.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Takita</surname>
<given-names>Marco A.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Forim</surname>
<given-names>Moacir R.</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Silva</surname>
<given-names>Luis F. C.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Coletta-Filho</surname>
<given-names>Helvécio D.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Machado</surname>
<given-names>Marcos A.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>de Souza</surname>
<given-names>Alessandra A.</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Centro de Citricultura Sylvio Moreira, Instituto Agronômico, Cordeirópolis, São Paulo, Brazil</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>Departamento de Genética e Biologia Molecular, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil</addr-line>
</aff>
<aff id="aff3"><label>3</label>
<addr-line>Departamento de Química, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Arnold</surname>
<given-names>Dawn</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1"><addr-line>University of the West of England, United Kingdom</addr-line>
</aff>
<author-notes><corresp id="cor1">* E-mail: <email>alessandra@centrodecitricultura.br</email>
</corresp>
<fn fn-type="conflict"><p><bold>Competing Interests: </bold>
The authors have declared that no competing interests exist.</p>
</fn>
<fn fn-type="con"><p>Conceived and designed the experiments: AADS HDCF MAM. Performed the experiments: LSM TEG MRF LFC. Analyzed the data: AADS HDCF LSM TEG MRF MAT. Contributed reagents/materials/analysis tools: AADS MAM. Wrote the manuscript: AADS LSM MAT. </p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>23</day>
<month>8</month>
<year>2013</year>
</pub-date>
<volume>8</volume>
<issue>8</issue>
<elocation-id>e72937</elocation-id>
<history><date date-type="received"><day>28</day>
<month>5</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>15</day>
<month>7</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-year>2013</copyright-year>
<copyright-holder>Muranaka et al</copyright-holder>
<license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p>
</license>
</permissions>
<abstract><p><italic><named-content content-type="genus-species">Xylella fastidiosa</named-content>
</italic>
is a plant pathogen bacterium that causes diseases in many different crops. In citrus, it causes Citrus Variegated Chlorosis (CVC). The mechanism of pathogenicity of this bacterium is associated with its capacity to colonize and form a biofilm in the xylem vessels of host plants, and there is not yet any method to directly reduce populations of this pathogen in the field. In this study, we investigated the inhibitory effect of N-Acetylcysteine (NAC), a cysteine analogue used mainly to treat human diseases, on <italic><named-content content-type="genus-species">X. fastidiosa</named-content>
</italic>
in different experimental conditions. Concentrations of NAC over 1 mg/mL reduced bacterial adhesion to glass surfaces, biofilm formation and the amount of exopolysaccharides (EPS). The minimal inhibitory concentration of NAC was 6 mg/mL. NAC was supplied to <italic><named-content content-type="genus-species">X. fastidiosa</named-content>
</italic>
-infected plants in hydroponics, fertigation, and adsorbed to organic fertilizer (NAC-Fertilizer). HPLC analysis indicated that plants absorbed NAC at concentrations of 0.48 and 2.4 mg/mL but not at 6 mg/mL. Sweet orange plants with CVC symptoms treated with NAC (0.48 and 2.4 mg/mL) in hydroponics showed clear symptom remission and reduction in bacterial population, as analyzed by quantitative PCR and bacterial isolation. Experiments using fertigation and NAC-Fertilizer were done to simulate a condition closer to that normally is used in the field. For both, significant symptom remission and a reduced bacterial growth rate were observed. Using NAC-Fertilizer the lag for resurgence of symptoms on leaves after interruption of the treatment increased to around eight months. This is the first report of the anti-bacterial effect of NAC against a phytopathogenic bacterium. The results obtained in this work together with the characteristics of this molecule indicate that the use of NAC in agriculture might be a new and sustainable strategy for controlling plant pathogenic bacteria.</p>
</abstract>
<funding-group><funding-statement>This work was supported by research grants from Fundação de Amparo à Pesquisa do Estado de São Paulo and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grants 2008/51274-5, 471448/2011-7 and INCT-Citros 08/57909-2, 573848/08-4). MAT, HDCF, MAM and AADS are recipients of research fellowships from CNPq. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement>
</funding-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Brésil</li>
</country>
<region><li>État de São Paulo</li>
</region>
<settlement><li>São Paulo</li>
</settlement>
</list>
<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Muranaka, Ligia S" sort="Muranaka, Ligia S" uniqKey="Muranaka L" first="Lígia S." last="Muranaka">Lígia S. Muranaka</name>
</region>
<name sortKey="Coletta Filho, Helvecio D" sort="Coletta Filho, Helvecio D" uniqKey="Coletta Filho H" first="Helvécio D." last="Coletta-Filho">Helvécio D. Coletta-Filho</name>
<name sortKey="De Souza, Alessandra A" sort="De Souza, Alessandra A" uniqKey="De Souza A" first="Alessandra A." last="De Souza">Alessandra A. De Souza</name>
<name sortKey="Forim, Moacir R" sort="Forim, Moacir R" uniqKey="Forim M" first="Moacir R." last="Forim">Moacir R. Forim</name>
<name sortKey="Giorgiano, Thais E" sort="Giorgiano, Thais E" uniqKey="Giorgiano T" first="Thais E." last="Giorgiano">Thais E. Giorgiano</name>
<name sortKey="Machado, Marcos A" sort="Machado, Marcos A" uniqKey="Machado M" first="Marcos A." last="Machado">Marcos A. Machado</name>
<name sortKey="Muranaka, Ligia S" sort="Muranaka, Ligia S" uniqKey="Muranaka L" first="Lígia S." last="Muranaka">Lígia S. Muranaka</name>
<name sortKey="Silva, Luis F C" sort="Silva, Luis F C" uniqKey="Silva L" first="Luis F. C." last="Silva">Luis F. C. Silva</name>
<name sortKey="Takita, Marco A" sort="Takita, Marco A" uniqKey="Takita M" first="Marco A." last="Takita">Marco A. Takita</name>
</country>
</tree>
</affiliations>
</record>
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