A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway
Identifieur interne : 001389 ( Ncbi/Merge ); précédent : 001388; suivant : 001390A R2R3-MYB Transcription Factor from Epimedium sagittatum Regulates the Flavonoid Biosynthetic Pathway
Auteurs : Wenjun Huang [République populaire de Chine] ; Wei Sun [République populaire de Chine] ; Haiyan Lv [République populaire de Chine] ; Ming Luo [République populaire de Chine] ; Shaohua Zeng [République populaire de Chine] ; Sitakanta Pattanaik [États-Unis] ; Ling Yuan [États-Unis] ; Ying Wang [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Herba epimedii (
Url:
DOI: 10.1371/journal.pone.0070778
PubMed: 23936468
PubMed Central: 3731294
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A R2R3-MYB Transcription Factor from <italic>Epimedium sagittatum</italic> Regulates the Flavonoid Biosynthetic Pathway</title><author><name sortKey="Huang, Wenjun" sort="Huang, Wenjun" uniqKey="Huang W" first="Wenjun" last="Huang">Wenjun Huang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Wei" sort="Sun, Wei" uniqKey="Sun W" first="Wei" last="Sun">Wei Sun</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Lv, Haiyan" sort="Lv, Haiyan" uniqKey="Lv H" first="Haiyan" last="Lv">Haiyan Lv</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Luo, Ming" sort="Luo, Ming" uniqKey="Luo M" first="Ming" last="Luo">Ming Luo</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Zeng, Shaohua" sort="Zeng, Shaohua" uniqKey="Zeng S" first="Shaohua" last="Zeng">Shaohua Zeng</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Pattanaik, Sitakanta" sort="Pattanaik, Sitakanta" uniqKey="Pattanaik S" first="Sitakanta" last="Pattanaik">Sitakanta Pattanaik</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky</wicri:regionArea><placeName><region type="state">Kentucky</region></placeName></affiliation></author><author><name sortKey="Yuan, Ling" sort="Yuan, Ling" uniqKey="Yuan L" first="Ling" last="Yuan">Ling Yuan</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky</wicri:regionArea><placeName><region type="state">Kentucky</region></placeName></affiliation></author><author><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23936468</idno><idno type="pmc">3731294</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731294</idno><idno type="RBID">PMC:3731294</idno><idno type="doi">10.1371/journal.pone.0070778</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">001155</idno><idno type="wicri:Area/Pmc/Curation">001154</idno><idno type="wicri:Area/Pmc/Checkpoint">000B75</idno><idno type="wicri:Area/Ncbi/Merge">001389</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A R2R3-MYB Transcription Factor from <italic>Epimedium sagittatum</italic> Regulates the Flavonoid Biosynthetic Pathway</title><author><name sortKey="Huang, Wenjun" sort="Huang, Wenjun" uniqKey="Huang W" first="Wenjun" last="Huang">Wenjun Huang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Wei" sort="Sun, Wei" uniqKey="Sun W" first="Wei" last="Sun">Wei Sun</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Lv, Haiyan" sort="Lv, Haiyan" uniqKey="Lv H" first="Haiyan" last="Lv">Haiyan Lv</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author><author><name sortKey="Luo, Ming" sort="Luo, Ming" uniqKey="Luo M" first="Ming" last="Luo">Ming Luo</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Zeng, Shaohua" sort="Zeng, Shaohua" uniqKey="Zeng S" first="Shaohua" last="Zeng">Shaohua Zeng</name><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Pattanaik, Sitakanta" sort="Pattanaik, Sitakanta" uniqKey="Pattanaik S" first="Sitakanta" last="Pattanaik">Sitakanta Pattanaik</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky</wicri:regionArea><placeName><region type="state">Kentucky</region></placeName></affiliation></author><author><name sortKey="Yuan, Ling" sort="Yuan, Ling" uniqKey="Yuan L" first="Ling" last="Yuan">Ling Yuan</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky</wicri:regionArea><placeName><region type="state">Kentucky</region></placeName></affiliation></author><author><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei</wicri:regionArea><wicri:noRegion>Hubei</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Herba epimedii (<italic>Epimedium</italic>), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. <italic>Epimedium</italic> species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in <italic>Epimedium</italic>. Here, we reported the isolation and functional characterization of the first <italic>R2R3-MYB</italic> TF (<italic>EsMYBA1</italic>) from <italic>Epimedium sagittatum</italic> (Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes <italic>Arabidopsis</italic> AtPAP1, apple MdMYB10 and legume MtLAP1. <italic>EsMYBA1</italic> was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of <italic>EsMYBA1</italic> resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (<italic>DFR</italic>) and anthocyanidin synthase (<italic>ANS</italic>). In both transgenic tobacco and <italic>Arabidopsis</italic>, overexpression of <italic>EsMYBA1</italic> induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes. Furthermore, transient expression of <italic>EsMYBA1</italic> in <italic>E. sagittatum</italic> leaves by <italic>Agrobacterium</italic> infiltration also induced anthocyanin accumulation in the wounded area. This first functional characterization of R2R3-MYB TFs in <italic>Epimedium</italic> species will promote further studies of the flavonoid biosynthesis and regulation in medicinal plants.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Winkel Shirley, B" uniqKey="Winkel Shirley B">B Winkel-Shirley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harborne, Jb" uniqKey="Harborne J">JB Harborne</name></author><author><name sortKey="Williams, Ca" uniqKey="Williams C">CA Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yao, Lh" uniqKey="Yao L">LH Yao</name></author><author><name sortKey="Jiang, Ym" uniqKey="Jiang Y">YM Jiang</name></author><author><name sortKey="Shi, J" uniqKey="Shi J">J Shi</name></author><author><name sortKey="Tomas Barberan, Fa" uniqKey="Tomas Barberan F">FA TomÁS-BarberÁN</name></author><author><name sortKey="Datta, N" uniqKey="Datta 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23936468</article-id><article-id pub-id-type="pmc">3731294</article-id><article-id pub-id-type="publisher-id">PONE-D-13-13580</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0070778</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Metabolism</subject><subj-group><subject>Biosynthesis</subject></subj-group></subj-group><subj-group><subject>Plant Biochemistry</subject><subj-group><subject>Secondary Metabolism</subject></subj-group></subj-group><subj-group><subject>Proteins</subject><subj-group><subject>Protein Interactions</subject></subj-group></subj-group></subj-group><subj-group><subject>Evolutionary Biology</subject><subj-group><subject>Evolutionary Systematics</subject><subj-group><subject>Phylogenetics</subject></subj-group></subj-group></subj-group><subj-group><subject>Plant Science</subject><subj-group><subject>Plant Biochemistry</subject><subj-group><subject>Secondary Metabolism</subject></subj-group></subj-group><subj-group><subject>Plant Physiology</subject></subj-group></subj-group><subj-group><subject>Proteomics</subject><subj-group><subject>Protein Interactions</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Medicine</subject><subj-group><subject>Complementary and Alternative Medicine</subject></subj-group></subj-group></article-categories><title-group><article-title>A R2R3-MYB Transcription Factor from <italic>Epimedium sagittatum</italic> Regulates the Flavonoid Biosynthetic Pathway</article-title><alt-title alt-title-type="running-head">Epimedium MYBA1 Regulates the Flavonoid Pathway</alt-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Huang</surname><given-names>Wenjun</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Sun</surname><given-names>Wei</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Lv</surname><given-names>Haiyan</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Luo</surname><given-names>Ming</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zeng</surname><given-names>Shaohua</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pattanaik</surname><given-names>Sitakanta</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Yuan</surname><given-names>Ling</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Ying</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky, United States of America</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Schiefelbein</surname><given-names>John</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>University of Michigan, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>yingwang@wbgcas.cn</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: YW. Performed the experiments: WH WS ML SP. Analyzed the data: WH WS LY. Contributed reagents/materials/analysis tools: HL SZ. Wrote the paper: WH YW.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>1</day><month>8</month><year>2013</year></pub-date><volume>8</volume><issue>8</issue><elocation-id>e70778</elocation-id><history><date date-type="received"><day>26</day><month>3</month><year>2013</year></date><date date-type="accepted"><day>21</day><month>6</month><year>2013</year></date></history><permissions><copyright-year>2013</copyright-year><copyright-holder>Huang et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><p>Herba epimedii (<italic>Epimedium</italic>), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. <italic>Epimedium</italic> species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in <italic>Epimedium</italic>. Here, we reported the isolation and functional characterization of the first <italic>R2R3-MYB</italic> TF (<italic>EsMYBA1</italic>) from <italic>Epimedium sagittatum</italic> (Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes <italic>Arabidopsis</italic> AtPAP1, apple MdMYB10 and legume MtLAP1. <italic>EsMYBA1</italic> was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of <italic>EsMYBA1</italic> resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (<italic>DFR</italic>) and anthocyanidin synthase (<italic>ANS</italic>). In both transgenic tobacco and <italic>Arabidopsis</italic>, overexpression of <italic>EsMYBA1</italic> induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes. Furthermore, transient expression of <italic>EsMYBA1</italic> in <italic>E. sagittatum</italic> leaves by <italic>Agrobacterium</italic> infiltration also induced anthocyanin accumulation in the wounded area. This first functional characterization of R2R3-MYB TFs in <italic>Epimedium</italic> species will promote further studies of the flavonoid biosynthesis and regulation in medicinal plants.</p></abstract><funding-group><funding-statement>This study was supported by grants from the National Natural Science Foundation of China (No. 31270340, 31200225), and CAS/SAFEA International Partnership Program for Creative Research Teams Project and Knowledge Innovation Project of The Chinese Academy of Sciences (KSCX2-EW-J-20). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="16"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Kentucky</li></region></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Wenjun" sort="Huang, Wenjun" uniqKey="Huang W" first="Wenjun" last="Huang">Wenjun Huang</name></noRegion><name sortKey="Luo, Ming" sort="Luo, Ming" uniqKey="Luo M" first="Ming" last="Luo">Ming Luo</name><name sortKey="Lv, Haiyan" sort="Lv, Haiyan" uniqKey="Lv H" first="Haiyan" last="Lv">Haiyan Lv</name><name sortKey="Sun, Wei" sort="Sun, Wei" uniqKey="Sun W" first="Wei" last="Sun">Wei Sun</name><name sortKey="Wang, Ying" sort="Wang, Ying" uniqKey="Wang Y" first="Ying" last="Wang">Ying Wang</name><name sortKey="Zeng, Shaohua" sort="Zeng, Shaohua" uniqKey="Zeng S" first="Shaohua" last="Zeng">Shaohua Zeng</name></country><country name="États-Unis"><region name="Kentucky"><name sortKey="Pattanaik, Sitakanta" sort="Pattanaik, Sitakanta" uniqKey="Pattanaik S" first="Sitakanta" last="Pattanaik">Sitakanta Pattanaik</name></region><name sortKey="Yuan, Ling" sort="Yuan, Ling" uniqKey="Yuan L" first="Ling" last="Yuan">Ling Yuan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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