Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality
Identifieur interne : 001308 ( Ncbi/Merge ); précédent : 001307; suivant : 001309Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, Diaphorina citri Disrupts Adult Wing Development and Increases Nymph Mortality
Auteurs : Ibrahim El-Shesheny [États-Unis, Égypte] ; Subhas Hajeri [États-Unis] ; Ibrahim El-Hawary [Égypte] ; Siddarame Gowda [États-Unis] ; Nabil Killiny [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium,
Url:
DOI: 10.1371/journal.pone.0065392
PubMed: 23734251
PubMed Central: 3667074
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, <italic>Diaphorina citri</italic> Disrupts Adult Wing Development and Increases Nymph Mortality</title><author><name sortKey="El Shesheny, Ibrahim" sort="El Shesheny, Ibrahim" uniqKey="El Shesheny I" first="Ibrahim" last="El-Shesheny">Ibrahim El-Shesheny</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta, Egypt</addr-line></nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta</wicri:regionArea><wicri:noRegion>Tanta</wicri:noRegion></affiliation></author><author><name sortKey="Hajeri, Subhas" sort="Hajeri, Subhas" uniqKey="Hajeri S" first="Subhas" last="Hajeri">Subhas Hajeri</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="El Hawary, Ibrahim" sort="El Hawary, Ibrahim" uniqKey="El Hawary I" first="Ibrahim" last="El-Hawary">Ibrahim El-Hawary</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta, Egypt</addr-line></nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta</wicri:regionArea><wicri:noRegion>Tanta</wicri:noRegion></affiliation></author><author><name sortKey="Gowda, Siddarame" sort="Gowda, Siddarame" uniqKey="Gowda S" first="Siddarame" last="Gowda">Siddarame Gowda</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Killiny, Nabil" sort="Killiny, Nabil" uniqKey="Killiny N" first="Nabil" last="Killiny">Nabil Killiny</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23734251</idno><idno type="pmc">3667074</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667074</idno><idno type="RBID">PMC:3667074</idno><idno type="doi">10.1371/journal.pone.0065392</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">001143</idno><idno type="wicri:Area/Pmc/Curation">001142</idno><idno type="wicri:Area/Pmc/Checkpoint">000987</idno><idno type="wicri:Area/Ncbi/Merge">001308</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, <italic>Diaphorina citri</italic> Disrupts Adult Wing Development and Increases Nymph Mortality</title><author><name sortKey="El Shesheny, Ibrahim" sort="El Shesheny, Ibrahim" uniqKey="El Shesheny I" first="Ibrahim" last="El-Shesheny">Ibrahim El-Shesheny</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta, Egypt</addr-line></nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta</wicri:regionArea><wicri:noRegion>Tanta</wicri:noRegion></affiliation></author><author><name sortKey="Hajeri, Subhas" sort="Hajeri, Subhas" uniqKey="Hajeri S" first="Subhas" last="Hajeri">Subhas Hajeri</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="El Hawary, Ibrahim" sort="El Hawary, Ibrahim" uniqKey="El Hawary I" first="Ibrahim" last="El-Hawary">Ibrahim El-Hawary</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta, Egypt</addr-line></nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta</wicri:regionArea><wicri:noRegion>Tanta</wicri:noRegion></affiliation></author><author><name sortKey="Gowda, Siddarame" sort="Gowda, Siddarame" uniqKey="Gowda S" first="Siddarame" last="Gowda">Siddarame Gowda</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Killiny, Nabil" sort="Killiny, Nabil" uniqKey="Killiny N" first="Nabil" last="Killiny">Nabil Killiny</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium, <italic>Candidatus</italic> Liberibacter asiaticus (<italic>C</italic>Las), the causal agent of HLB. Silencing genes by RNA interference (RNAi) is a promising tool to explore gene functions as well as control pests. In the current study, abnormal wing disc (<italic>awd</italic>) gene associated with wing development in insects is used to interfere with the flight of psyllids. Our study showed that transcription of <italic>awd</italic> is development-dependent and the highest level was found in the last instar (5<sup>th</sup>) of the nymphal stage. Micro-application (topical application) of dsRNA to 5<sup>th</sup> instar of nymphs caused significant nymphal mortality and adult wing-malformation. These adverse effects in ACP were positively correlated with the amounts of dsRNA used. A qRT-PCR analysis confirmed the dsRNA-mediated transcriptional down-regulation of the <italic>awd</italic> gene. Significant down-regulation was required to induce a wing-malformed phenotype. No effect was found when dsRNA-<italic>gfp</italic> was used, indicating the specific effect of dsRNA<italic>-awd</italic>. Our findings suggest a role for <italic>awd</italic> in ACP wing development and metamorphosis. <italic>awd</italic> could serve as a potential target for insect management either via direct application of dsRNA or by producing transgenic plants expressing dsRNA<italic>-awd</italic>. 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article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23734251</article-id><article-id pub-id-type="pmc">3667074</article-id><article-id pub-id-type="publisher-id">PONE-D-13-09416</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0065392</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Agriculture</subject><subj-group><subject>Agrochemicals</subject><subj-group><subject>Crop Diseases</subject></subj-group></subj-group><subj-group><subject>Crops</subject><subj-group><subject>Crop Diseases</subject></subj-group></subj-group><subj-group><subject>Pest Control</subject></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Biochemistry</subject><subj-group><subject>Nucleic Acids</subject><subj-group><subject>RNA</subject><subj-group><subject>RNA interference</subject></subj-group></subj-group></subj-group></subj-group><subj-group><subject>Genetics</subject><subj-group><subject>Gene Expression</subject><subj-group><subject>RNA interference</subject></subj-group></subj-group><subj-group><subject>Heredity</subject><subj-group><subject>Phenotypes</subject></subj-group></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Silencing Abnormal Wing Disc Gene of the Asian Citrus Psyllid, <italic>Diaphorina citri</italic> Disrupts Adult Wing Development and Increases Nymph Mortality</article-title><alt-title alt-title-type="running-head">Silencing <italic>awd</italic> of <italic>Diaphorina citri</italic></alt-title></title-group><contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>El-Shesheny</surname><given-names>Ibrahim</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author" equal-contrib="yes"><name><surname>Hajeri</surname><given-names>Subhas</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>El-Hawary</surname><given-names>Ibrahim</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Gowda</surname><given-names>Siddarame</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Killiny</surname><given-names>Nabil</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, Florida, United States of America</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta, Egypt</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Niedz</surname><given-names>Randall P.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>United States Department of Agriculture, United States of America</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>nabilkilliny@ufl.edu</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: NK SG. Performed the experiments: IEs SH. Analyzed the data: NK IEs IEh. Contributed reagents/materials/analysis tools: NK IEs SH. Wrote the paper: NK IEs SG IEh.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>29</day><month>5</month><year>2013</year></pub-date><volume>8</volume><issue>5</issue><elocation-id>e65392</elocation-id><history><date date-type="received"><day>5</day><month>3</month><year>2013</year></date><date date-type="accepted"><day>29</day><month>4</month><year>2013</year></date></history><permissions><copyright-year>2013</copyright-year><license><license-p>This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.</license-p></license></permissions><abstract><p>Huanglongbing (HLB) causes considerable economic losses to citrus industries worldwide. Its management depends on controlling of the Asian citrus Psyllid (ACP), the vector of the bacterium, <italic>Candidatus</italic> Liberibacter asiaticus (<italic>C</italic>Las), the causal agent of HLB. Silencing genes by RNA interference (RNAi) is a promising tool to explore gene functions as well as control pests. In the current study, abnormal wing disc (<italic>awd</italic>) gene associated with wing development in insects is used to interfere with the flight of psyllids. Our study showed that transcription of <italic>awd</italic> is development-dependent and the highest level was found in the last instar (5<sup>th</sup>) of the nymphal stage. Micro-application (topical application) of dsRNA to 5<sup>th</sup> instar of nymphs caused significant nymphal mortality and adult wing-malformation. These adverse effects in ACP were positively correlated with the amounts of dsRNA used. A qRT-PCR analysis confirmed the dsRNA-mediated transcriptional down-regulation of the <italic>awd</italic> gene. Significant down-regulation was required to induce a wing-malformed phenotype. No effect was found when dsRNA-<italic>gfp</italic> was used, indicating the specific effect of dsRNA<italic>-awd</italic>. Our findings suggest a role for <italic>awd</italic> in ACP wing development and metamorphosis. <italic>awd</italic> could serve as a potential target for insect management either via direct application of dsRNA or by producing transgenic plants expressing dsRNA<italic>-awd</italic>. These strategies will help to mitigate HLB by controlling ACP.</p></abstract><funding-group><funding-statement>Ibrahim El-Shesheny was supported with a scholarship from the Egyptian government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="8"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>Égypte</li><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="El Shesheny, Ibrahim" sort="El Shesheny, Ibrahim" uniqKey="El Shesheny I" first="Ibrahim" last="El-Shesheny">Ibrahim El-Shesheny</name></region><name sortKey="Gowda, Siddarame" sort="Gowda, Siddarame" uniqKey="Gowda S" first="Siddarame" last="Gowda">Siddarame Gowda</name><name sortKey="Hajeri, Subhas" sort="Hajeri, Subhas" uniqKey="Hajeri S" first="Subhas" last="Hajeri">Subhas Hajeri</name><name sortKey="Killiny, Nabil" sort="Killiny, Nabil" uniqKey="Killiny N" first="Nabil" last="Killiny">Nabil Killiny</name></country><country name="Égypte"><noRegion><name sortKey="El Shesheny, Ibrahim" sort="El Shesheny, Ibrahim" uniqKey="El Shesheny I" first="Ibrahim" last="El-Shesheny">Ibrahim El-Shesheny</name></noRegion><name sortKey="El Hawary, Ibrahim" sort="El Hawary, Ibrahim" uniqKey="El Hawary I" first="Ibrahim" last="El-Hawary">Ibrahim El-Hawary</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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