Detection of warfare agents in liquid foods using the brine shrimp lethality assay.
Identifieur interne : 000D22 ( Ncbi/Merge ); précédent : 000D21; suivant : 000D23Detection of warfare agents in liquid foods using the brine shrimp lethality assay.
Auteurs : Stephen E. Lumor [États-Unis] ; Francisco Diez-Gonzalez ; Theodore P. LabuzaSource :
- Journal of food science [ 1750-3841 ]
English descriptors
- KwdEn :
- Animals, Artemia (drug effects), Beverages (analysis), Biological Assay, Biological Warfare Agents, Chemical Warfare Agents (analysis), Chemical Warfare Agents (toxicity), Citrus sinensis (chemistry), Cyanides (analysis), Cyanides (toxicity), Food Contamination, Food Inspection (methods), Fruit (chemistry), Larva (drug effects), Lethal Dose 50, Limit of Detection, Milk (chemistry), Osmolar Concentration, Pesticides (analysis), Pesticides (toxicity), T-2 Toxin (analysis), T-2 Toxin (toxicity), Time Factors, Trimethylsilyl Compounds (analysis), Trimethylsilyl Compounds (toxicity).
- MESH :
- chemical , analysis : Chemical Warfare Agents, Cyanides, Pesticides, T-2 Toxin, Trimethylsilyl Compounds.
- chemical , toxicity : Chemical Warfare Agents, Cyanides, Pesticides, T-2 Toxin, Trimethylsilyl Compounds.
- chemical : Biological Warfare Agents.
- analysis : Beverages.
- chemistry : Citrus sinensis, Fruit, Milk.
- drug effects : Artemia, Larva.
- methods : Food Inspection.
- Animals, Biological Assay, Food Contamination, Lethal Dose 50, Limit of Detection, Osmolar Concentration, Time Factors.
Abstract
The brine shrimp lethality assay (BSLA) was used for rapid and non-specific detection of biological and chemical warfare agents at concentrations considerably below that which will cause harm to humans. Warfare agents detected include T-2 toxin, trimethylsilyl cyanide, and commercially available pesticides such as dichlorvos, diazinon, dursban, malathion, and parathion. The assay was performed by introducing 50 μL of milk or orange juice contaminated with each analyte into vials containing 10 freshly hatched brine shrimp nauplii in seawater. This was incubated at 28 °C for 24 h, after which mortality was determined. Mortality was converted to probits and the LC(50) was determined for each analyte by plotting probits of mortality against analyte concentration (log(10)). Our findings were the following: (1) the lethal effects of toxins dissolved in milk were observed, with T-2 toxin being the most lethal and malathion being the least, (2) except for parathion, the dosage (based on LC(50)) of analyte in a cup of milk (200 mL) consumed by a 6-y-old (20 kg) was less than the respective published rat LD(50) values, and (3) the BSLA was only suitable for detecting toxins dissolved in orange juice if incubation time was reduced to 6 h. Our results support the application of the BSLA for routine, rapid, and non-specific prescreening of liquid foods for possible sabotage by an employee or an intentional bioterrorist act. Practical Application: The findings of this study strongly indicate that the brine shrimp lethality assay can be adapted for nonspecific detection of warfare agents or toxins in food at any point during food production and distribution.
DOI: 10.1111/j.1750-3841.2010.01966.x
PubMed: 21535725
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Lumor</name><affiliation wicri:level="2"><nlm:affiliation>Dept. of Food Science and Nutrition, Univ. of Minnesota, 1334 Eckles Ave., Saint Paul, MN 55108, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Dept. of Food Science and Nutrition, Univ. of Minnesota, 1334 Eckles Ave., Saint Paul, MN 55108</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Diez Gonzalez, Francisco" sort="Diez Gonzalez, Francisco" uniqKey="Diez Gonzalez F" first="Francisco" last="Diez-Gonzalez">Francisco Diez-Gonzalez</name></author><author><name sortKey="Labuza, Theodore P" sort="Labuza, Theodore P" uniqKey="Labuza T" first="Theodore P" last="Labuza">Theodore P. 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Lumor</name><affiliation wicri:level="2"><nlm:affiliation>Dept. of Food Science and Nutrition, Univ. of Minnesota, 1334 Eckles Ave., Saint Paul, MN 55108, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Dept. of Food Science and Nutrition, Univ. of Minnesota, 1334 Eckles Ave., Saint Paul, MN 55108</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Diez Gonzalez, Francisco" sort="Diez Gonzalez, Francisco" uniqKey="Diez Gonzalez F" first="Francisco" last="Diez-Gonzalez">Francisco Diez-Gonzalez</name></author><author><name sortKey="Labuza, Theodore P" sort="Labuza, Theodore P" uniqKey="Labuza T" first="Theodore P" last="Labuza">Theodore P. Labuza</name></author></analytic><series><title level="j">Journal of food science</title><idno type="eISSN">1750-3841</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Artemia (drug effects)</term><term>Beverages (analysis)</term><term>Biological Assay</term><term>Biological Warfare Agents</term><term>Chemical Warfare Agents (analysis)</term><term>Chemical Warfare Agents (toxicity)</term><term>Citrus sinensis (chemistry)</term><term>Cyanides (analysis)</term><term>Cyanides (toxicity)</term><term>Food Contamination</term><term>Food Inspection (methods)</term><term>Fruit (chemistry)</term><term>Larva (drug effects)</term><term>Lethal Dose 50</term><term>Limit of Detection</term><term>Milk (chemistry)</term><term>Osmolar Concentration</term><term>Pesticides (analysis)</term><term>Pesticides (toxicity)</term><term>T-2 Toxin (analysis)</term><term>T-2 Toxin (toxicity)</term><term>Time Factors</term><term>Trimethylsilyl Compounds (analysis)</term><term>Trimethylsilyl Compounds (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Chemical Warfare Agents</term><term>Cyanides</term><term>Pesticides</term><term>T-2 Toxin</term><term>Trimethylsilyl Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Chemical Warfare Agents</term><term>Cyanides</term><term>Pesticides</term><term>T-2 Toxin</term><term>Trimethylsilyl Compounds</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biological Warfare Agents</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term><term>Milk</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Artemia</term><term>Larva</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Food Inspection</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biological Assay</term><term>Food Contamination</term><term>Lethal Dose 50</term><term>Limit of Detection</term><term>Osmolar Concentration</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The brine shrimp lethality assay (BSLA) was used for rapid and non-specific detection of biological and chemical warfare agents at concentrations considerably below that which will cause harm to humans. Warfare agents detected include T-2 toxin, trimethylsilyl cyanide, and commercially available pesticides such as dichlorvos, diazinon, dursban, malathion, and parathion. The assay was performed by introducing 50 μL of milk or orange juice contaminated with each analyte into vials containing 10 freshly hatched brine shrimp nauplii in seawater. This was incubated at 28 °C for 24 h, after which mortality was determined. Mortality was converted to probits and the LC(50) was determined for each analyte by plotting probits of mortality against analyte concentration (log(10)). Our findings were the following: (1) the lethal effects of toxins dissolved in milk were observed, with T-2 toxin being the most lethal and malathion being the least, (2) except for parathion, the dosage (based on LC(50)) of analyte in a cup of milk (200 mL) consumed by a 6-y-old (20 kg) was less than the respective published rat LD(50) values, and (3) the BSLA was only suitable for detecting toxins dissolved in orange juice if incubation time was reduced to 6 h. Our results support the application of the BSLA for routine, rapid, and non-specific prescreening of liquid foods for possible sabotage by an employee or an intentional bioterrorist act. Practical Application: The findings of this study strongly indicate that the brine shrimp lethality assay can be adapted for nonspecific detection of warfare agents or toxins in food at any point during food production and distribution.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21535725</PMID><DateCreated><Year>2011</Year><Month>5</Month><Day>3</Day></DateCreated><DateCompleted><Year>2011</Year><Month>09</Month><Day>29</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1750-3841</ISSN><JournalIssue CitedMedium="Internet"><Volume>76</Volume><Issue>1</Issue><PubDate><MedlineDate>2011 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Journal of food science</Title><ISOAbbreviation>J. Food Sci.</ISOAbbreviation></Journal><ArticleTitle>Detection of warfare agents in liquid foods using the brine shrimp lethality assay.</ArticleTitle><Pagination><MedlinePgn>T16-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1750-3841.2010.01966.x</ELocationID><Abstract><AbstractText>The brine shrimp lethality assay (BSLA) was used for rapid and non-specific detection of biological and chemical warfare agents at concentrations considerably below that which will cause harm to humans. Warfare agents detected include T-2 toxin, trimethylsilyl cyanide, and commercially available pesticides such as dichlorvos, diazinon, dursban, malathion, and parathion. The assay was performed by introducing 50 μL of milk or orange juice contaminated with each analyte into vials containing 10 freshly hatched brine shrimp nauplii in seawater. This was incubated at 28 °C for 24 h, after which mortality was determined. Mortality was converted to probits and the LC(50) was determined for each analyte by plotting probits of mortality against analyte concentration (log(10)). Our findings were the following: (1) the lethal effects of toxins dissolved in milk were observed, with T-2 toxin being the most lethal and malathion being the least, (2) except for parathion, the dosage (based on LC(50)) of analyte in a cup of milk (200 mL) consumed by a 6-y-old (20 kg) was less than the respective published rat LD(50) values, and (3) the BSLA was only suitable for detecting toxins dissolved in orange juice if incubation time was reduced to 6 h. Our results support the application of the BSLA for routine, rapid, and non-specific prescreening of liquid foods for possible sabotage by an employee or an intentional bioterrorist act. Practical Application: The findings of this study strongly indicate that the brine shrimp lethality assay can be adapted for nonspecific detection of warfare agents or toxins in food at any point during food production and distribution.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lumor</LastName><ForeName>Stephen E</ForeName><Initials>SE</Initials><AffiliationInfo><Affiliation>Dept. of Food Science and Nutrition, Univ. of Minnesota, 1334 Eckles Ave., Saint Paul, MN 55108, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Diez-Gonzalez</LastName><ForeName>Francisco</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Labuza</LastName><ForeName>Theodore P</ForeName><Initials>TP</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType 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Labuza</name></noCountry><country name="États-Unis"><region name="Minnesota"><name sortKey="Lumor, Stephen E" sort="Lumor, Stephen E" uniqKey="Lumor S" first="Stephen E" last="Lumor">Stephen E. Lumor</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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