Isolation and characterization of beneficial bacteria associated with citrus roots in Florida.
Identifieur interne : 000D03 ( Ncbi/Merge ); précédent : 000D02; suivant : 000D04Isolation and characterization of beneficial bacteria associated with citrus roots in Florida.
Auteurs : Pankaj Trivedi [États-Unis] ; Timothy Spann ; Nian WangSource :
- Microbial ecology [ 1432-184X ] ; 2011.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (metabolism), Azides (chemistry), Bacteria (genetics), Bacteria (growth & development), Bacteria (isolation & purification), Bacteria (metabolism), Bacterial Proteins (metabolism), Biological Control Agents, Chitinase (biosynthesis), Citrus sinensis (growth & development), Citrus sinensis (microbiology), Crops, Agricultural (growth & development), Crops, Agricultural (microbiology), Florida, Indoleacetic Acids (metabolism), Nitrogen Fixation, Plant Diseases (microbiology), Plant Roots (microbiology), Polymerase Chain Reaction, RNA, Ribosomal, 16S (genetics), Siderophores (biosynthesis), Soil Microbiology.
- MESH :
- chemical , biosynthesis : Chitinase, Siderophores.
- chemical , chemistry : Azides.
- chemical , genetics : RNA, Ribosomal, 16S.
- chemical , metabolism : Anti-Bacterial Agents, Bacterial Proteins, Indoleacetic Acids.
- geographic : Florida.
- genetics : Bacteria.
- growth & development : Bacteria, Citrus sinensis, Crops, Agricultural.
- isolation & purification : Bacteria.
- metabolism : Bacteria.
- microbiology : Citrus sinensis, Crops, Agricultural, Plant Diseases, Plant Roots.
- chemical : Biological Control Agents, Nitrogen Fixation, Polymerase Chain Reaction, Soil Microbiology.
Abstract
Cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. Citrus roots from Huanglongbing (HLB) diseased symptomatic and asymptomatic citrus were used in this study. A total of 227 and 125 morphologically distinct colonies were isolated and characterized from HLB asymptomatic and symptomatic trees, respectively. We observed that the frequency of bacterial isolates possessing various plant beneficial properties was significantly higher in the asymptomatic samples. A total of 39 bacterial isolates showing a minimum of five beneficial traits related to mineral nutrition [phosphate (P) solubilization, siderophore production, nitrogen (N) fixation], development [indole acetic acid (IAA) synthesis], health [production of antibiotic and lytic enzymes (chitinase)], induction of systemic resistance [salicylic acid (SA) production], stress relief [production of 1-amino-cyclopropane-1-carboxylate deaminase] and production of quorum sensing [N-acyl homoserine lactones] signals were characterized. A bioassay using ethidium monoazide (EMA)-qPCR was developed to select bacteria antagonistic to Candidatus Liberibacter asiaticus. Using the modified EMA-qPCR assay, we found six bacterial isolates showing maximum similarity to Paenibacillus validus, Lysinibacillus fusiformis, Bacillus licheniformis, Pseudomonas putida, Microbacterium oleivorans, and Serratia plymutica could significantly reduce the population of viable Ca. L. asiaticus in HLB symptomatic leaf samples. In conclusion, we have isolated and characterized multiple beneficial bacterial strains from citrus roots which have the potential to enhance plant growth and suppress diseases.
DOI: 10.1007/s00248-011-9822-y
PubMed: 21360139
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000717
- to stream PubMed, to step Curation: 000717
- to stream PubMed, to step Checkpoint: 000717
Links to Exploration step
pubmed:21360139Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and characterization of beneficial bacteria associated with citrus roots in Florida.</title><author><name sortKey="Trivedi, Pankaj" sort="Trivedi, Pankaj" uniqKey="Trivedi P" first="Pankaj" last="Trivedi">Pankaj Trivedi</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Spann, Timothy" sort="Spann, Timothy" uniqKey="Spann T" first="Timothy" last="Spann">Timothy Spann</name></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21360139</idno><idno type="pmid">21360139</idno><idno type="doi">10.1007/s00248-011-9822-y</idno><idno type="wicri:Area/PubMed/Corpus">000717</idno><idno type="wicri:Area/PubMed/Curation">000717</idno><idno type="wicri:Area/PubMed/Checkpoint">000717</idno><idno type="wicri:Area/Ncbi/Merge">000D03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and characterization of beneficial bacteria associated with citrus roots in Florida.</title><author><name sortKey="Trivedi, Pankaj" sort="Trivedi, Pankaj" uniqKey="Trivedi P" first="Pankaj" last="Trivedi">Pankaj Trivedi</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Spann, Timothy" sort="Spann, Timothy" uniqKey="Spann T" first="Timothy" last="Spann">Timothy Spann</name></author><author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name></author></analytic><series><title level="j">Microbial ecology</title><idno type="eISSN">1432-184X</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (metabolism)</term><term>Azides (chemistry)</term><term>Bacteria (genetics)</term><term>Bacteria (growth & development)</term><term>Bacteria (isolation & purification)</term><term>Bacteria (metabolism)</term><term>Bacterial Proteins (metabolism)</term><term>Biological Control Agents</term><term>Chitinase (biosynthesis)</term><term>Citrus sinensis (growth & development)</term><term>Citrus sinensis (microbiology)</term><term>Crops, Agricultural (growth & development)</term><term>Crops, Agricultural (microbiology)</term><term>Florida</term><term>Indoleacetic Acids (metabolism)</term><term>Nitrogen Fixation</term><term>Plant Diseases (microbiology)</term><term>Plant Roots (microbiology)</term><term>Polymerase Chain Reaction</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Siderophores (biosynthesis)</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Chitinase</term><term>Siderophores</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Azides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Bacterial Proteins</term><term>Indoleacetic Acids</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Florida</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Bacteria</term><term>Citrus sinensis</term><term>Crops, Agricultural</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Crops, Agricultural</term><term>Plant Diseases</term><term>Plant Roots</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biological Control Agents</term><term>Nitrogen Fixation</term><term>Polymerase Chain Reaction</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. Citrus roots from Huanglongbing (HLB) diseased symptomatic and asymptomatic citrus were used in this study. A total of 227 and 125 morphologically distinct colonies were isolated and characterized from HLB asymptomatic and symptomatic trees, respectively. We observed that the frequency of bacterial isolates possessing various plant beneficial properties was significantly higher in the asymptomatic samples. A total of 39 bacterial isolates showing a minimum of five beneficial traits related to mineral nutrition [phosphate (P) solubilization, siderophore production, nitrogen (N) fixation], development [indole acetic acid (IAA) synthesis], health [production of antibiotic and lytic enzymes (chitinase)], induction of systemic resistance [salicylic acid (SA) production], stress relief [production of 1-amino-cyclopropane-1-carboxylate deaminase] and production of quorum sensing [N-acyl homoserine lactones] signals were characterized. A bioassay using ethidium monoazide (EMA)-qPCR was developed to select bacteria antagonistic to Candidatus Liberibacter asiaticus. Using the modified EMA-qPCR assay, we found six bacterial isolates showing maximum similarity to Paenibacillus validus, Lysinibacillus fusiformis, Bacillus licheniformis, Pseudomonas putida, Microbacterium oleivorans, and Serratia plymutica could significantly reduce the population of viable Ca. L. asiaticus in HLB symptomatic leaf samples. In conclusion, we have isolated and characterized multiple beneficial bacterial strains from citrus roots which have the potential to enhance plant growth and suppress diseases.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21360139</PMID><DateCreated><Year>2011</Year><Month>8</Month><Day>12</Day></DateCreated><DateCompleted><Year>2011</Year><Month>12</Month><Day>15</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-184X</ISSN><JournalIssue CitedMedium="Internet"><Volume>62</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Microbial ecology</Title><ISOAbbreviation>Microb. Ecol.</ISOAbbreviation></Journal><ArticleTitle>Isolation and characterization of beneficial bacteria associated with citrus roots in Florida.</ArticleTitle><Pagination><MedlinePgn>324-36</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00248-011-9822-y</ELocationID><Abstract><AbstractText>Cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. Citrus roots from Huanglongbing (HLB) diseased symptomatic and asymptomatic citrus were used in this study. A total of 227 and 125 morphologically distinct colonies were isolated and characterized from HLB asymptomatic and symptomatic trees, respectively. We observed that the frequency of bacterial isolates possessing various plant beneficial properties was significantly higher in the asymptomatic samples. A total of 39 bacterial isolates showing a minimum of five beneficial traits related to mineral nutrition [phosphate (P) solubilization, siderophore production, nitrogen (N) fixation], development [indole acetic acid (IAA) synthesis], health [production of antibiotic and lytic enzymes (chitinase)], induction of systemic resistance [salicylic acid (SA) production], stress relief [production of 1-amino-cyclopropane-1-carboxylate deaminase] and production of quorum sensing [N-acyl homoserine lactones] signals were characterized. A bioassay using ethidium monoazide (EMA)-qPCR was developed to select bacteria antagonistic to Candidatus Liberibacter asiaticus. Using the modified EMA-qPCR assay, we found six bacterial isolates showing maximum similarity to Paenibacillus validus, Lysinibacillus fusiformis, Bacillus licheniformis, Pseudomonas putida, Microbacterium oleivorans, and Serratia plymutica could significantly reduce the population of viable Ca. L. asiaticus in HLB symptomatic leaf samples. In conclusion, we have isolated and characterized multiple beneficial bacterial strains from citrus roots which have the potential to enhance plant growth and suppress diseases.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Trivedi</LastName><ForeName>Pankaj</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Spann</LastName><ForeName>Timothy</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Nian</ForeName><Initials>N</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>Mar</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Microb Ecol</MedlineTA><NlmUniqueID>7500663</NlmUniqueID><ISSNLinking>0095-3628</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001386">Azides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D061046">Biological Control Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007210">Indoleacetic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017262">Siderophores</NameOfSubstance></Chemical><Chemical><RegistryNumber>69498-50-6</RegistryNumber><NameOfSubstance UI="C014535">8-azidoethidium</NameOfSubstance></Chemical><Chemical><RegistryNumber>6U1S09C61L</RegistryNumber><NameOfSubstance UI="C030737">indoleacetic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001386" MajorTopicYN="N">Azides</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D061046" MajorTopicYN="N">Biological Control Agents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018556" MajorTopicYN="N">Crops, Agricultural</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005431" MajorTopicYN="N" Type="Geographic">Florida</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007210" MajorTopicYN="N">Indoleacetic Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009586" MajorTopicYN="N">Nitrogen Fixation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017262" MajorTopicYN="N">Siderophores</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>12</Month><Day>5</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>2</Month><Day>7</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>3</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21360139</ArticleId><ArticleId IdType="doi">10.1007/s00248-011-9822-y</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><noCountry><name sortKey="Spann, Timothy" sort="Spann, Timothy" uniqKey="Spann T" first="Timothy" last="Spann">Timothy Spann</name><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name></noCountry><country name="États-Unis"><region name="Floride"><name sortKey="Trivedi, Pankaj" sort="Trivedi, Pankaj" uniqKey="Trivedi P" first="Pankaj" last="Trivedi">Pankaj Trivedi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D03 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000D03 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:21360139
|texte= Isolation and characterization of beneficial bacteria associated with citrus roots in Florida.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:21360139" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |