Development of chemiluminescence enzyme-linked immunosorbent assay for the screening of metolcarb and carbaryl in orange juice, cabbage and cucumber.
Identifieur interne : 000B16 ( Ncbi/Merge ); précédent : 000B15; suivant : 000B17Development of chemiluminescence enzyme-linked immunosorbent assay for the screening of metolcarb and carbaryl in orange juice, cabbage and cucumber.
Auteurs : J W Sun [République populaire de Chine] ; Y. Zhang ; S. WangSource :
- Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment [ 1944-0057 ] ; 2010.
English descriptors
- KwdEn :
- Beverages (analysis), Brassica (chemistry), Carbaryl (chemistry), Citrus sinensis, Cucumis sativus (chemistry), Enzyme-Linked Immunosorbent Assay (methods), Fruit, Insecticides (chemistry), Luminescent Measurements, Molecular Structure, Pesticide Residues, Phenylcarbamates (chemistry), Reproducibility of Results, Sensitivity and Specificity.
- MESH :
- chemical , chemistry : Carbaryl, Insecticides, Phenylcarbamates.
- analysis : Beverages.
- chemistry : Brassica, Cucumis sativus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Citrus sinensis, Fruit, Luminescent Measurements, Molecular Structure, Pesticide Residues, Reproducibility of Results, Sensitivity and Specificity.
Abstract
A direct competitive enzyme-linked immunosorbent assay (ELISA) with enhanced chemiluminescent detection for simultaneous detection of metolcarb and carbaryl in agricultural products is reported. The concentrations of coating antibodies and enzyme tracer, as well as the effects of pH and methanol concentrations, were investigated. Under optimum conditions, the IC(50) of metolcarb and carbaryl were 4 and 0.3 microg kg(-1), respectively. Detection of metolcarb and carbaryl in fruit juices and vegetables was accomplished using simple, rapid and efficient extraction methods. Recoveries (n = 3) of spiked samples ranged 75-108% for metolcarb and 74-98% for carbaryl. Validation of the enhanced chemiluminescent immunosorbent assay was conducted by comparison of results with HPLC. The correlation between the data obtained using ELISA with enhanced chemiluminescent detection and HPLC was good (n = 9). Therefore, the enhanced chemiluminescent ELISA was suitable for quantitative simultaneously determination of metolcarb and carbaryl residue in agricultural products.
DOI: 10.1080/19440040903403016
PubMed: 20155539
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pubmed:20155539Le document en format XML
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<author><name sortKey="Sun, J W" sort="Sun, J W" uniqKey="Sun J" first="J W" last="Sun">J W Sun</name>
<affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457</wicri:regionArea>
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<author><name sortKey="Zhang, Y" sort="Zhang, Y" uniqKey="Zhang Y" first="Y" last="Zhang">Y. Zhang</name>
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<author><name sortKey="Wang, S" sort="Wang, S" uniqKey="Wang S" first="S" last="Wang">S. Wang</name>
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<term>Brassica (chemistry)</term>
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<term>Citrus sinensis</term>
<term>Cucumis sativus (chemistry)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Fruit</term>
<term>Insecticides (chemistry)</term>
<term>Luminescent Measurements</term>
<term>Molecular Structure</term>
<term>Pesticide Residues</term>
<term>Phenylcarbamates (chemistry)</term>
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<term>Sensitivity and Specificity</term>
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<front><div type="abstract" xml:lang="en">A direct competitive enzyme-linked immunosorbent assay (ELISA) with enhanced chemiluminescent detection for simultaneous detection of metolcarb and carbaryl in agricultural products is reported. The concentrations of coating antibodies and enzyme tracer, as well as the effects of pH and methanol concentrations, were investigated. Under optimum conditions, the IC(50) of metolcarb and carbaryl were 4 and 0.3 microg kg(-1), respectively. Detection of metolcarb and carbaryl in fruit juices and vegetables was accomplished using simple, rapid and efficient extraction methods. Recoveries (n = 3) of spiked samples ranged 75-108% for metolcarb and 74-98% for carbaryl. Validation of the enhanced chemiluminescent immunosorbent assay was conducted by comparison of results with HPLC. The correlation between the data obtained using ELISA with enhanced chemiluminescent detection and HPLC was good (n = 9). Therefore, the enhanced chemiluminescent ELISA was suitable for quantitative simultaneously determination of metolcarb and carbaryl residue in agricultural products.</div>
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<ArticleTitle>Development of chemiluminescence enzyme-linked immunosorbent assay for the screening of metolcarb and carbaryl in orange juice, cabbage and cucumber.</ArticleTitle>
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<Abstract><AbstractText>A direct competitive enzyme-linked immunosorbent assay (ELISA) with enhanced chemiluminescent detection for simultaneous detection of metolcarb and carbaryl in agricultural products is reported. The concentrations of coating antibodies and enzyme tracer, as well as the effects of pH and methanol concentrations, were investigated. Under optimum conditions, the IC(50) of metolcarb and carbaryl were 4 and 0.3 microg kg(-1), respectively. Detection of metolcarb and carbaryl in fruit juices and vegetables was accomplished using simple, rapid and efficient extraction methods. Recoveries (n = 3) of spiked samples ranged 75-108% for metolcarb and 74-98% for carbaryl. Validation of the enhanced chemiluminescent immunosorbent assay was conducted by comparison of results with HPLC. The correlation between the data obtained using ELISA with enhanced chemiluminescent detection and HPLC was good (n = 9). Therefore, the enhanced chemiluminescent ELISA was suitable for quantitative simultaneously determination of metolcarb and carbaryl residue in agricultural products.</AbstractText>
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