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High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.

Identifieur interne : 000923 ( Ncbi/Merge ); précédent : 000922; suivant : 000924

High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.

Auteurs : Maria Luiza Peixoto De Oliveira [Brésil] ; Vicente J. Febres ; Marcio Gilberto Cardoso Costa ; Gloria A. Moore ; Wagner Campos Otoni

Source :

Plant cell reports [ 1432-203X ] ; 2009.

RBID : pubmed:19048258

English descriptors

KwdEn :
- Citrus (genetics), Gene Transfer Techniques, Plants, Genetically Modified (genetics), Rhizobium (genetics), Sonication, Transformation, Genetic, Vacuum.
MESH :
- genetics : Citrus, Plants, Genetically Modified, Rhizobium.
- Gene Transfer Techniques, Sonication, Transformation, Genetic, Vacuum.

Abstract

An improved method for the Agrobacterium infiltration of epicotyl segments of 'Pineapple' sweet orange [Citrus sinensis (L.) Osbeck] and 'Swingle' citrumelo [Citrus paradisi Macf. X Poncirus trifoliata (L.) Raf.] was developed in order to increase transformation frequency. Sonication-assisted Agrobacterium-mediated transformation (SAAT), vacuum infiltration, and a combination of the two procedures were compared with conventional Agrobacterium-mediated inoculation method ('dipping' method). It was observed that the morphogenic potential of the epicotyl segments decreased as the duration of SAAT and vacuum treatments increased. Transient GUS expression was not affected by the different SAAT treatments, but it was significantly enhanced by the vacuum infiltration treatments. The highest transformation efficiencies were obtained when the explants were subjected to a combination of SAAT for 2 s followed by 10 min of vacuum infiltration. PCR and Southern blot analysis of the uidA gene were used to confirm the integration of the transgenes. The transformation frequencies achieved in this study (8.4% for 'Pineapple' sweet orange and 11.2% for 'Swingle' citrumelo) are the highest ones reported for both cultivars.

DOI: 10.1007/s00299-008-0646-2
PubMed: 19048258

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Le document en format XML

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<author><name sortKey="De Oliveira, Maria Luiza Peixoto" sort="De Oliveira, Maria Luiza Peixoto" uniqKey="De Oliveira M" first="Maria Luiza Peixoto" last="De Oliveira">Maria Luiza Peixoto De Oliveira</name>
<affiliation wicri:level="2"><nlm:affiliation>Plant Biology Department, Plant Tissue Culture Laboratory/BIOAGRO, Federal University of Viçosa, Viçosa, MG, Brazil.</nlm:affiliation>
<country xml:lang="fr">Brésil</country>
<wicri:regionArea>Plant Biology Department, Plant Tissue Culture Laboratory/BIOAGRO, Federal University of Viçosa, Viçosa, MG</wicri:regionArea>
<placeName><region type="state">Minas Gerais</region>
</placeName>
</affiliation>
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<author><name sortKey="Febres, Vicente J" sort="Febres, Vicente J" uniqKey="Febres V" first="Vicente J" last="Febres">Vicente J. Febres</name>
</author>
<author><name sortKey="Costa, Marcio Gilberto Cardoso" sort="Costa, Marcio Gilberto Cardoso" uniqKey="Costa M" first="Marcio Gilberto Cardoso" last="Costa">Marcio Gilberto Cardoso Costa</name>
</author>
<author><name sortKey="Moore, Gloria A" sort="Moore, Gloria A" uniqKey="Moore G" first="Gloria A" last="Moore">Gloria A. Moore</name>
</author>
<author><name sortKey="Otoni, Wagner Campos" sort="Otoni, Wagner Campos" uniqKey="Otoni W" first="Wagner Campos" last="Otoni">Wagner Campos Otoni</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.</title>
<author><name sortKey="De Oliveira, Maria Luiza Peixoto" sort="De Oliveira, Maria Luiza Peixoto" uniqKey="De Oliveira M" first="Maria Luiza Peixoto" last="De Oliveira">Maria Luiza Peixoto De Oliveira</name>
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<author><name sortKey="Febres, Vicente J" sort="Febres, Vicente J" uniqKey="Febres V" first="Vicente J" last="Febres">Vicente J. Febres</name>
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<author><name sortKey="Costa, Marcio Gilberto Cardoso" sort="Costa, Marcio Gilberto Cardoso" uniqKey="Costa M" first="Marcio Gilberto Cardoso" last="Costa">Marcio Gilberto Cardoso Costa</name>
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<author><name sortKey="Moore, Gloria A" sort="Moore, Gloria A" uniqKey="Moore G" first="Gloria A" last="Moore">Gloria A. Moore</name>
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<author><name sortKey="Otoni, Wagner Campos" sort="Otoni, Wagner Campos" uniqKey="Otoni W" first="Wagner Campos" last="Otoni">Wagner Campos Otoni</name>
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<series><title level="j">Plant cell reports</title>
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<term>Gene Transfer Techniques</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Rhizobium (genetics)</term>
<term>Sonication</term>
<term>Transformation, Genetic</term>
<term>Vacuum</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term>
<term>Plants, Genetically Modified</term>
<term>Rhizobium</term>
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<keywords scheme="MESH" xml:lang="en"><term>Gene Transfer Techniques</term>
<term>Sonication</term>
<term>Transformation, Genetic</term>
<term>Vacuum</term>
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<front><div type="abstract" xml:lang="en">An improved method for the Agrobacterium infiltration of epicotyl segments of 'Pineapple' sweet orange [Citrus sinensis (L.) Osbeck] and 'Swingle' citrumelo [Citrus paradisi Macf. X Poncirus trifoliata (L.) Raf.] was developed in order to increase transformation frequency. Sonication-assisted Agrobacterium-mediated transformation (SAAT), vacuum infiltration, and a combination of the two procedures were compared with conventional Agrobacterium-mediated inoculation method ('dipping' method). It was observed that the morphogenic potential of the epicotyl segments decreased as the duration of SAAT and vacuum treatments increased. Transient GUS expression was not affected by the different SAAT treatments, but it was significantly enhanced by the vacuum infiltration treatments. The highest transformation efficiencies were obtained when the explants were subjected to a combination of SAAT for 2 s followed by 10 min of vacuum infiltration. PCR and Southern blot analysis of the uidA gene were used to confirm the integration of the transgenes. The transformation frequencies achieved in this study (8.4% for 'Pineapple' sweet orange and 11.2% for 'Swingle' citrumelo) are the highest ones reported for both cultivars.</div>
</front>
</TEI>
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<DateCreated><Year>2009</Year>
<Month>2</Month>
<Day>26</Day>
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<DateCompleted><Year>2009</Year>
<Month>06</Month>
<Day>11</Day>
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<DateRevised><Year>2009</Year>
<Month>2</Month>
<Day>26</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>28</Volume>
<Issue>3</Issue>
<PubDate><Year>2009</Year>
<Month>Mar</Month>
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<Title>Plant cell reports</Title>
<ISOAbbreviation>Plant Cell Rep.</ISOAbbreviation>
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<ArticleTitle>High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.</ArticleTitle>
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<ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-008-0646-2</ELocationID>
<Abstract><AbstractText>An improved method for the Agrobacterium infiltration of epicotyl segments of 'Pineapple' sweet orange [Citrus sinensis (L.) Osbeck] and 'Swingle' citrumelo [Citrus paradisi Macf. X Poncirus trifoliata (L.) Raf.] was developed in order to increase transformation frequency. Sonication-assisted Agrobacterium-mediated transformation (SAAT), vacuum infiltration, and a combination of the two procedures were compared with conventional Agrobacterium-mediated inoculation method ('dipping' method). It was observed that the morphogenic potential of the epicotyl segments decreased as the duration of SAAT and vacuum treatments increased. Transient GUS expression was not affected by the different SAAT treatments, but it was significantly enhanced by the vacuum infiltration treatments. The highest transformation efficiencies were obtained when the explants were subjected to a combination of SAAT for 2 s followed by 10 min of vacuum infiltration. PCR and Southern blot analysis of the uidA gene were used to confirm the integration of the transgenes. The transformation frequencies achieved in this study (8.4% for 'Pineapple' sweet orange and 11.2% for 'Swingle' citrumelo) are the highest ones reported for both cultivars.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>de Oliveira</LastName>
<ForeName>Maria Luiza Peixoto</ForeName>
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<Author ValidYN="Y"><LastName>Febres</LastName>
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<Author ValidYN="Y"><LastName>Costa</LastName>
<ForeName>Marcio Gilberto Cardoso</ForeName>
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<Author ValidYN="Y"><LastName>Moore</LastName>
<ForeName>Gloria A</ForeName>
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<Author ValidYN="Y"><LastName>Otoni</LastName>
<ForeName>Wagner Campos</ForeName>
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<ArticleDate DateType="Electronic"><Year>2008</Year>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D002957" MajorTopicYN="N">Citrus</DescriptorName>
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<name sortKey="Febres, Vicente J" sort="Febres, Vicente J" uniqKey="Febres V" first="Vicente J" last="Febres">Vicente J. Febres</name>
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High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.

High-efficiency Agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration.

Source :

English descriptors

Abstract

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Links to Exploration step

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