An engineered closterovirus RNA replicon and analysis of heterologous terminal sequences for replication
Identifieur interne : 000001 ( Ncbi/Merge ); précédent : 000000; suivant : 000002An engineered closterovirus RNA replicon and analysis of heterologous terminal sequences for replication
Auteurs : T. Satyanarayana [États-Unis] ; S. Gowda [États-Unis] ; V. P. Boyko [États-Unis] ; M. R. Albiach-Marti [États-Unis] ; M. Mawassi [États-Unis] ; J. Navas-Castillo [États-Unis] ; A. V. Karasev [États-Unis] ; V. Dolja [États-Unis] ; M. E. Hilf [États-Unis] ; D. J. Lewandowski [États-Unis] ; P. Moreno [Espagne] ; M. Bar-Joseph [Israël] ; S. M. Garnsey [États-Unis] ; W. O. Dawson [États-Unis]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1999.
Abstract
Citrus tristeza virus (CTV) populations in citrus trees are unusually complex mixtures of viral genotypes and defective RNAs developed during the long-term vegetative propagation of the virus and by additional mixing by aphid transmission. The viral replication process allows the maintenance of minor amounts of disparate genotypes and defective RNAs in these populations. CTV is a member of the
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PubMed: 10377432
PubMed Central: 22103
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Gowda</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Boyko, V P" sort="Boyko, V P" uniqKey="Boyko V" first="V. P." last="Boyko">V. P. 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Navas-Castillo</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Karasev, A V" sort="Karasev, A V" uniqKey="Karasev A" first="A. V." last="Karasev">A. V. Karasev</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Dolja, V" sort="Dolja, V" uniqKey="Dolja V" first="V." last="Dolja">V. Dolja</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Oregon</region></placeName><wicri:cityArea>Department of Botany and Plant Pathology, Oregon State University, Corvallis</wicri:cityArea></affiliation></author><author><name sortKey="Hilf, M E" sort="Hilf, M E" uniqKey="Hilf M" first="M. E." last="Hilf">M. E. Hilf</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando, FL 32803;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando</wicri:cityArea></affiliation></author><author><name sortKey="Lewandowski, D J" sort="Lewandowski, D J" uniqKey="Lewandowski D" first="D. J." last="Lewandowski">D. J. Lewandowski</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Moreno, P" sort="Moreno, P" uniqKey="Moreno P" first="P." last="Moreno">P. Moreno</name><affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="N0x8a59a20.0x9be8cc0">Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Bar Joseph, M" sort="Bar Joseph, M" uniqKey="Bar Joseph M" first="M." last="Bar-Joseph">M. Bar-Joseph</name><affiliation wicri:level="1"><nlm:aff id="N0x8a59a20.0x9be8cc0">The Volcani Institute, Bet-Dagan 50250, Israel</nlm:aff><country xml:lang="fr">Israël</country><wicri:regionArea>The Volcani Institute, Bet-Dagan 50250</wicri:regionArea><wicri:noRegion>Bet-Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Garnsey, S M" sort="Garnsey, S M" uniqKey="Garnsey S" first="S. M." last="Garnsey">S. M. Garnsey</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando, FL 32803;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando</wicri:cityArea></affiliation></author><author><name sortKey="Dawson, W O" sort="Dawson, W O" uniqKey="Dawson W" first="W. O." last="Dawson">W. O. Dawson</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">10377432</idno><idno type="pmc">22103</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC22103</idno><idno type="RBID">PMC:22103</idno><date when="1999">1999</date><idno type="wicri:Area/Pmc/Corpus">000543</idno><idno type="wicri:Area/Pmc/Curation">000542</idno><idno type="wicri:Area/Pmc/Checkpoint">001231</idno><idno type="wicri:Area/Ncbi/Merge">000001</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An engineered closterovirus RNA replicon and analysis of heterologous terminal sequences for replication</title><author><name sortKey="Satyanarayana, T" sort="Satyanarayana, T" uniqKey="Satyanarayana T" first="T." last="Satyanarayana">T. Satyanarayana</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Gowda, S" sort="Gowda, S" uniqKey="Gowda S" first="S." last="Gowda">S. Gowda</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Boyko, V P" sort="Boyko, V P" uniqKey="Boyko V" first="V. P." last="Boyko">V. P. Boyko</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Albiach Marti, M R" sort="Albiach Marti, M R" uniqKey="Albiach Marti M" first="M. R." last="Albiach-Marti">M. R. Albiach-Marti</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Mawassi, M" sort="Mawassi, M" uniqKey="Mawassi M" first="M." last="Mawassi">M. Mawassi</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Navas Castillo, J" sort="Navas Castillo, J" uniqKey="Navas Castillo J" first="J." last="Navas-Castillo">J. Navas-Castillo</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Karasev, A V" sort="Karasev, A V" uniqKey="Karasev A" first="A. V." last="Karasev">A. V. Karasev</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Dolja, V" sort="Dolja, V" uniqKey="Dolja V" first="V." last="Dolja">V. Dolja</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Oregon</region></placeName><wicri:cityArea>Department of Botany and Plant Pathology, Oregon State University, Corvallis</wicri:cityArea></affiliation></author><author><name sortKey="Hilf, M E" sort="Hilf, M E" uniqKey="Hilf M" first="M. E." last="Hilf">M. E. Hilf</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando, FL 32803;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando</wicri:cityArea></affiliation></author><author><name sortKey="Lewandowski, D J" sort="Lewandowski, D J" uniqKey="Lewandowski D" first="D. J." last="Lewandowski">D. J. Lewandowski</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author><author><name sortKey="Moreno, P" sort="Moreno, P" uniqKey="Moreno P" first="P." last="Moreno">P. Moreno</name><affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="N0x8a59a20.0x9be8cc0">Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Bar Joseph, M" sort="Bar Joseph, M" uniqKey="Bar Joseph M" first="M." last="Bar-Joseph">M. Bar-Joseph</name><affiliation wicri:level="1"><nlm:aff id="N0x8a59a20.0x9be8cc0">The Volcani Institute, Bet-Dagan 50250, Israel</nlm:aff><country xml:lang="fr">Israël</country><wicri:regionArea>The Volcani Institute, Bet-Dagan 50250</wicri:regionArea><wicri:noRegion>Bet-Dagan 50250</wicri:noRegion></affiliation></author><author><name sortKey="Garnsey, S M" sort="Garnsey, S M" uniqKey="Garnsey S" first="S. M." last="Garnsey">S. M. Garnsey</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando, FL 32803;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando</wicri:cityArea></affiliation></author><author><name sortKey="Dawson, W O" sort="Dawson, W O" uniqKey="Dawson W" first="W. O." last="Dawson">W. O. Dawson</name><affiliation wicri:level="2"><nlm:aff id="N0x8a59a20.0x9be8cc0">Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred</wicri:cityArea></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="1999">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Citrus tristeza virus (CTV) populations in citrus trees are unusually complex mixtures of viral genotypes and defective RNAs developed during the long-term vegetative propagation of the virus and by additional mixing by aphid transmission. The viral replication process allows the maintenance of minor amounts of disparate genotypes and defective RNAs in these populations. CTV is a member of the <italic>Closteroviridae</italic> possessing a positive-stranded RNA genome of ≈20 kilobases that expresses the replicase-associated genes as an ≈400-kDa polyprotein and the remaining 10 3′ genes through subgenomic mRNAs. A full-length cDNA clone of CTV was generated from which RNA transcripts capable of replication in protoplasts were derived. The large size of cDNA hampered its use as a genetic system. Deletion of 10 3′ genes resulted in an efficient RNA replicon that was easy to manipulate. To investigate the origin and maintenance of the genotypes in CTV populations, we tested the CTV replicase for its acceptance of divergent sequences by creating chimeric replicons with heterologous termini and examining their ability to replicate. Exchange of the similar 3′ termini resulted in efficient replication whereas substitution of the divergent (up to 58% difference in sequence) 5′ termini resulted in reduced but significant replication, generally in proportion to the extent of sequence divergence.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-id journal-id-type="publisher-id">PNAS</journal-id><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn><publisher><publisher-name>The National Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">10377432</article-id><article-id pub-id-type="pmc">22103</article-id><article-id pub-id-type="publisher-id">1571</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject><subj-group><subject>Genetics</subject></subj-group></subj-group></article-categories><title-group><article-title>An engineered closterovirus RNA replicon and analysis of heterologous terminal sequences for replication</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Satyanarayana</surname><given-names>T.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Gowda</surname><given-names>S.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Boyko</surname><given-names>V. P.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Albiach-Marti</surname><given-names>M. R.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Mawassi</surname><given-names>M.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Navas-Castillo</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Karasev</surname><given-names>A. V.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Dolja</surname><given-names>V.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">†</xref></contrib><contrib contrib-type="author"><name><surname>Hilf</surname><given-names>M. E.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">‡</xref></contrib><contrib contrib-type="author"><name><surname>Lewandowski</surname><given-names>D. J.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref></contrib><contrib contrib-type="author"><name><surname>Moreno</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">§</xref></contrib><contrib contrib-type="author"><name><surname>Bar-Joseph</surname><given-names>M.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">¶</xref></contrib><contrib contrib-type="author"><name><surname>Garnsey</surname><given-names>S. M.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">‡</xref></contrib><contrib contrib-type="author"><name><surname>Dawson</surname><given-names>W. O.</given-names></name><xref ref-type="aff" rid="N0x8a59a20.0x9be8cc0">*</xref><xref ref-type="author-notes" rid="FN155">‖</xref></contrib></contrib-group><aff id="N0x8a59a20.0x9be8cc0"><label>*</label>Department of Plant Pathology, University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850;<label>†</label>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331;<label>‡</label>U.S. Horticultural Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Orlando, FL 32803;<label>§</label>Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain; and<label>¶</label>The Volcani Institute, Bet-Dagan 50250, Israel</aff><author-notes><fn id="FN155"><label>‖</label><p>To whom reprint requests should be addressed at: CREC, 700 Experiment Station Road, Lake Alfred, FL 33850. e-mail: <email>wodtmv@icon.lal.ufl.edu</email>.</p></fn><fn><p>Communicated by George Bruening, University of California, Davis, CA</p></fn></author-notes><pub-date pub-type="ppub"><day>22</day><month>6</month><year>1999</year></pub-date><volume>96</volume><issue>13</issue><fpage>7433</fpage><lpage>7438</lpage><history><date date-type="received"><day>9</day><month>3</month><year>1999</year></date><date date-type="accepted"><day>20</day><month>4</month><year>1999</year></date></history><copyright-statement>Copyright © 1999, The National Academy of Sciences</copyright-statement><copyright-year>1999</copyright-year><abstract><p>Citrus tristeza virus (CTV) populations in citrus trees are unusually complex mixtures of viral genotypes and defective RNAs developed during the long-term vegetative propagation of the virus and by additional mixing by aphid transmission. The viral replication process allows the maintenance of minor amounts of disparate genotypes and defective RNAs in these populations. CTV is a member of the <italic>Closteroviridae</italic> possessing a positive-stranded RNA genome of ≈20 kilobases that expresses the replicase-associated genes as an ≈400-kDa polyprotein and the remaining 10 3′ genes through subgenomic mRNAs. A full-length cDNA clone of CTV was generated from which RNA transcripts capable of replication in protoplasts were derived. The large size of cDNA hampered its use as a genetic system. Deletion of 10 3′ genes resulted in an efficient RNA replicon that was easy to manipulate. To investigate the origin and maintenance of the genotypes in CTV populations, we tested the CTV replicase for its acceptance of divergent sequences by creating chimeric replicons with heterologous termini and examining their ability to replicate. Exchange of the similar 3′ termini resulted in efficient replication whereas substitution of the divergent (up to 58% difference in sequence) 5′ termini resulted in reduced but significant replication, generally in proportion to the extent of sequence divergence.</p></abstract></article-meta></front></pmc><affiliations><list><country><li>Espagne</li><li>Israël</li><li>États-Unis</li></country><region><li>Floride</li><li>Oregon</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Satyanarayana, T" sort="Satyanarayana, T" uniqKey="Satyanarayana T" first="T." last="Satyanarayana">T. Satyanarayana</name></region><name sortKey="Albiach Marti, M R" sort="Albiach Marti, M R" uniqKey="Albiach Marti M" first="M. R." last="Albiach-Marti">M. R. Albiach-Marti</name><name sortKey="Boyko, V P" sort="Boyko, V P" uniqKey="Boyko V" first="V. P." last="Boyko">V. P. Boyko</name><name sortKey="Dawson, W O" sort="Dawson, W O" uniqKey="Dawson W" first="W. O." last="Dawson">W. O. Dawson</name><name sortKey="Dolja, V" sort="Dolja, V" uniqKey="Dolja V" first="V." last="Dolja">V. Dolja</name><name sortKey="Garnsey, S M" sort="Garnsey, S M" uniqKey="Garnsey S" first="S. M." last="Garnsey">S. M. Garnsey</name><name sortKey="Gowda, S" sort="Gowda, S" uniqKey="Gowda S" first="S." last="Gowda">S. Gowda</name><name sortKey="Hilf, M E" sort="Hilf, M E" uniqKey="Hilf M" first="M. E." last="Hilf">M. E. Hilf</name><name sortKey="Karasev, A V" sort="Karasev, A V" uniqKey="Karasev A" first="A. V." last="Karasev">A. V. Karasev</name><name sortKey="Lewandowski, D J" sort="Lewandowski, D J" uniqKey="Lewandowski D" first="D. J." last="Lewandowski">D. J. Lewandowski</name><name sortKey="Mawassi, M" sort="Mawassi, M" uniqKey="Mawassi M" first="M." last="Mawassi">M. Mawassi</name><name sortKey="Navas Castillo, J" sort="Navas Castillo, J" uniqKey="Navas Castillo J" first="J." last="Navas-Castillo">J. Navas-Castillo</name></country><country name="Espagne"><noRegion><name sortKey="Moreno, P" sort="Moreno, P" uniqKey="Moreno P" first="P." last="Moreno">P. Moreno</name></noRegion></country><country name="Israël"><noRegion><name sortKey="Bar Joseph, M" sort="Bar Joseph, M" uniqKey="Bar Joseph M" first="M." last="Bar-Joseph">M. Bar-Joseph</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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