Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.).
Identifieur interne : 002060 ( Ncbi/Checkpoint ); précédent : 002059; suivant : 002061Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.).
Auteurs : Hao Wu [États-Unis] ; Yosvanis Acanda [États-Unis] ; Hongge Jia [États-Unis] ; Nian Wang [États-Unis] ; Janice Zale [États-Unis]Source :
- Plant cell reports [ 1432-203X ] ; 2016.
Abstract
The development of transgenic citrus plants by the biolistic method. A protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. Carrizo (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.) and plant regeneration is described. Immature epicotyl explants were bombarded with a vector containing the nptII selectable marker and the gfp reporter. The number of independent, stably transformed tissues/total number of explants, recorded by monitoring GFP fluorescence 4 weeks after bombardment was substantial at 18.4 %, and some fluorescing tissues regenerated into shoots. Fluorescing GFP, putative transgenic shoots were micro-grafted onto immature Carrizo rootstocks in vitro, confirmed by PCR amplification of nptII and gfp coding regions, followed by secondary grafting onto older rootstocks grown in soil. Southern blot analysis indicated that all the fluorescing shoots were transgenic. Multiple and single copies of nptII integrations were confirmed in five regenerated transgenic lines. There is potential to develop a higher throughput biolistics transformation system by optimizing the tissue culture medium to improve shoot regeneration and narrowing the window for plant sampling. This system will be appropriate for transformation with minimal cassettes.
DOI: 10.1007/s00299-016-2010-2
PubMed: 27277128
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000017
- to stream PubMed, to step Curation: 000017
- to stream PubMed, to step Checkpoint: 000017
- to stream Ncbi, to step Merge: 002060
- to stream Ncbi, to step Curation: 002060
Links to Exploration step
pubmed:27277128Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.).</title>
<author><name sortKey="Wu, Hao" sort="Wu, Hao" uniqKey="Wu H" first="Hao" last="Wu">Hao Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA. whao@ufl.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Acanda, Yosvanis" sort="Acanda, Yosvanis" uniqKey="Acanda Y" first="Yosvanis" last="Acanda">Yosvanis Acanda</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jia, Hongge" sort="Jia, Hongge" uniqKey="Jia H" first="Hongge" last="Jia">Hongge Jia</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zale, Janice" sort="Zale, Janice" uniqKey="Zale J" first="Janice" last="Zale">Janice Zale</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA. jzale@ufl.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:27277128</idno>
<idno type="pmid">27277128</idno>
<idno type="doi">10.1007/s00299-016-2010-2</idno>
<idno type="wicri:Area/PubMed/Corpus">000017</idno>
<idno type="wicri:Area/PubMed/Curation">000017</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000017</idno>
<idno type="wicri:Area/Ncbi/Merge">002060</idno>
<idno type="wicri:Area/Ncbi/Curation">002060</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002060</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.).</title>
<author><name sortKey="Wu, Hao" sort="Wu, Hao" uniqKey="Wu H" first="Hao" last="Wu">Hao Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA. whao@ufl.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Acanda, Yosvanis" sort="Acanda, Yosvanis" uniqKey="Acanda Y" first="Yosvanis" last="Acanda">Yosvanis Acanda</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jia, Hongge" sort="Jia, Hongge" uniqKey="Jia H" first="Hongge" last="Jia">Hongge Jia</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zale, Janice" sort="Zale, Janice" uniqKey="Zale J" first="Janice" last="Zale">Janice Zale</name>
<affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA. jzale@ufl.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850</wicri:regionArea>
<wicri:noRegion>33850</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Plant cell reports</title>
<idno type="eISSN">1432-203X</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The development of transgenic citrus plants by the biolistic method. A protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. Carrizo (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.) and plant regeneration is described. Immature epicotyl explants were bombarded with a vector containing the nptII selectable marker and the gfp reporter. The number of independent, stably transformed tissues/total number of explants, recorded by monitoring GFP fluorescence 4 weeks after bombardment was substantial at 18.4 %, and some fluorescing tissues regenerated into shoots. Fluorescing GFP, putative transgenic shoots were micro-grafted onto immature Carrizo rootstocks in vitro, confirmed by PCR amplification of nptII and gfp coding regions, followed by secondary grafting onto older rootstocks grown in soil. Southern blot analysis indicated that all the fluorescing shoots were transgenic. Multiple and single copies of nptII integrations were confirmed in five regenerated transgenic lines. There is potential to develop a higher throughput biolistics transformation system by optimizing the tissue culture medium to improve shoot regeneration and narrowing the window for plant sampling. This system will be appropriate for transformation with minimal cassettes.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Wu, Hao" sort="Wu, Hao" uniqKey="Wu H" first="Hao" last="Wu">Hao Wu</name>
</noRegion>
<name sortKey="Acanda, Yosvanis" sort="Acanda, Yosvanis" uniqKey="Acanda Y" first="Yosvanis" last="Acanda">Yosvanis Acanda</name>
<name sortKey="Jia, Hongge" sort="Jia, Hongge" uniqKey="Jia H" first="Hongge" last="Jia">Hongge Jia</name>
<name sortKey="Wang, Nian" sort="Wang, Nian" uniqKey="Wang N" first="Nian" last="Wang">Nian Wang</name>
<name sortKey="Zale, Janice" sort="Zale, Janice" uniqKey="Zale J" first="Janice" last="Zale">Janice Zale</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002060 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 002060 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:27277128 |texte= Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.). }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:27277128" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a OrangerV1
![]() | This area was generated with Dilib version V0.6.25. | ![]() |