Reliable molecular identification of nine tropical whitefly species
Identifieur interne : 001985 ( Ncbi/Checkpoint ); précédent : 001984; suivant : 001986Reliable molecular identification of nine tropical whitefly species
Auteurs : Tatiana M. Ovalle [Colombie] ; Soroush Parsa [Colombie, Pérou] ; Maria P. Hernández [Colombie] ; Luis A. Becerra Lopez-Lavalle [Colombie, Pérou]Source :
- Ecology and Evolution [ 2045-7758 ] ; 2014.
Abstract
The identification of whitefly species in adult stage is problematic. Morphological differentiation of pupae is one of the better methods for determining identity of species, but it may vary depending on the host plant on which they develop which can lead to misidentifications and erroneous naming of new species. Polymerase chain reaction (PCR) fragment amplified from the mitochondrial cytochrome oxidase I (
Url:
DOI: 10.1002/ece3.1204
PubMed: 25614792
PubMed Central: 4301044
Affiliations:
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PMC:4301044Le document en format XML
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<series><title level="j">Ecology and Evolution</title>
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<front><div type="abstract" xml:lang="en"><p>The identification of whitefly species in adult stage is problematic. Morphological differentiation of pupae is one of the better methods for determining identity of species, but it may vary depending on the host plant on which they develop which can lead to misidentifications and erroneous naming of new species. Polymerase chain reaction (PCR) fragment amplified from the mitochondrial cytochrome oxidase I (<italic>COI</italic>
) gene is often used for mitochondrial haplotype identification that can be associated with specific species. Our objective was to compare morphometric traits against DNA barcode sequences to develop and implement a diagnostic molecular kit based on a RFLP-PCR method using the <italic>COI</italic>
gene for the rapid identification of whiteflies. This study will allow for the rapid diagnosis of the diverse community of whiteflies attacking plants of economic interest in Colombia. It also provides access to the <italic>COI</italic>
sequence that can be used to develop predator conservation techniques by establishing which predators have a trophic linkage with the focal whitefly pest species.</p>
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<affiliations><list><country><li>Colombie</li>
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<tree><country name="Colombie"><noRegion><name sortKey="Ovalle, Tatiana M" sort="Ovalle, Tatiana M" uniqKey="Ovalle T" first="Tatiana M" last="Ovalle">Tatiana M. Ovalle</name>
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<name sortKey="Becerra Lopez Lavalle, Luis A" sort="Becerra Lopez Lavalle, Luis A" uniqKey="Becerra Lopez Lavalle L" first="Luis A" last="Becerra Lopez-Lavalle">Luis A. Becerra Lopez-Lavalle</name>
<name sortKey="Hernandez, Maria P" sort="Hernandez, Maria P" uniqKey="Hernandez M" first="Maria P" last="Hernández">Maria P. Hernández</name>
<name sortKey="Parsa, Soroush" sort="Parsa, Soroush" uniqKey="Parsa S" first="Soroush" last="Parsa">Soroush Parsa</name>
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<country name="Pérou"><noRegion><name sortKey="Parsa, Soroush" sort="Parsa, Soroush" uniqKey="Parsa S" first="Soroush" last="Parsa">Soroush Parsa</name>
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<name sortKey="Becerra Lopez Lavalle, Luis A" sort="Becerra Lopez Lavalle, Luis A" uniqKey="Becerra Lopez Lavalle L" first="Luis A" last="Becerra Lopez-Lavalle">Luis A. Becerra Lopez-Lavalle</name>
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