Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.
Identifieur interne : 001475 ( Ncbi/Checkpoint ); précédent : 001474; suivant : 001476Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.
Auteurs : L. Pe A [Espagne] ; M. Cervera ; J. Juárez ; A. Navarro ; J A Pina ; N. Durán-Vila ; L. NavarroSource :
- Plant cell reports [ 0721-7714 ] ; 1995.
Abstract
Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and ß-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.
DOI: 10.1007/BF00232724
PubMed: 24194307
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000F40
- to stream PubMed, to step Curation: 000F40
- to stream PubMed, to step Checkpoint: 000F40
- to stream Ncbi, to step Merge: 001475
- to stream Ncbi, to step Curation: 001475
Links to Exploration step
pubmed:24194307Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.</title><author><name sortKey="Pe A, L" sort="Pe A, L" uniqKey="Pe A L" first="L" last="Pe A">L. Pe A</name><affiliation wicri:level="1"><nlm:affiliation>Dpto. de protección vegetal y biotecnología, Institute Valenciano de Investigaciones Agrarias, Apartado oficial, E-46113, Moncada, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Dpto. de protección vegetal y biotecnología, Institute Valenciano de Investigaciones Agrarias, Apartado oficial, E-46113, Moncada</wicri:regionArea><wicri:noRegion>Moncada</wicri:noRegion></affiliation></author><author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M" last="Cervera">M. Cervera</name></author><author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J" last="Juárez">J. Juárez</name></author><author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A" last="Navarro">A. Navarro</name></author><author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J A" last="Pina">J A Pina</name></author><author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N" last="Durán-Vila">N. Durán-Vila</name></author><author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L" last="Navarro">L. Navarro</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:24194307</idno><idno type="pmid">24194307</idno><idno type="doi">10.1007/BF00232724</idno><idno type="wicri:Area/PubMed/Corpus">000F40</idno><idno type="wicri:Area/PubMed/Curation">000F40</idno><idno type="wicri:Area/PubMed/Checkpoint">000F40</idno><idno type="wicri:Area/Ncbi/Merge">001475</idno><idno type="wicri:Area/Ncbi/Curation">001475</idno><idno type="wicri:Area/Ncbi/Checkpoint">001475</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.</title><author><name sortKey="Pe A, L" sort="Pe A, L" uniqKey="Pe A L" first="L" last="Pe A">L. Pe A</name><affiliation wicri:level="1"><nlm:affiliation>Dpto. de protección vegetal y biotecnología, Institute Valenciano de Investigaciones Agrarias, Apartado oficial, E-46113, Moncada, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Dpto. de protección vegetal y biotecnología, Institute Valenciano de Investigaciones Agrarias, Apartado oficial, E-46113, Moncada</wicri:regionArea><wicri:noRegion>Moncada</wicri:noRegion></affiliation></author><author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M" last="Cervera">M. Cervera</name></author><author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J" last="Juárez">J. Juárez</name></author><author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A" last="Navarro">A. Navarro</name></author><author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J A" last="Pina">J A Pina</name></author><author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N" last="Durán-Vila">N. Durán-Vila</name></author><author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L" last="Navarro">L. Navarro</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and ß-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M" last="Cervera">M. Cervera</name><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N" last="Durán-Vila">N. Durán-Vila</name><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J" last="Juárez">J. Juárez</name><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A" last="Navarro">A. Navarro</name><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L" last="Navarro">L. Navarro</name><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J A" last="Pina">J A Pina</name></noCountry><country name="Espagne"><noRegion><name sortKey="Pe A, L" sort="Pe A, L" uniqKey="Pe A L" first="L" last="Pe A">L. Pe A</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001475 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 001475 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:24194307
|texte= Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:24194307" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |