Characterization of gibberellin 20-oxidases in the citrus hybrid Carrizo citrange.
Identifieur interne : 000944 ( Ncbi/Checkpoint ); précédent : 000943; suivant : 000945Characterization of gibberellin 20-oxidases in the citrus hybrid Carrizo citrange.
Auteurs : Laura Huerta [Espagne] ; Andres Garcia-Lor ; Jose L. Garcia-MartinezSource :
- Tree physiology [ 0829-318X ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence, Chromatography, High Pressure Liquid, Citrus (enzymology), Citrus (genetics), Cloning, Molecular, Hybridization, Genetic, Mixed Function Oxygenases (chemistry), Mixed Function Oxygenases (genetics), Mixed Function Oxygenases (metabolism), Molecular Sequence Data, Phylogeny, Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), RNA, Messenger (metabolism), Sequence Alignment, Sequence Analysis, Protein.
- MESH :
- chemical , chemistry : Mixed Function Oxygenases, Plant Proteins.
- enzymology : Citrus.
- genetics : Citrus, Mixed Function Oxygenases, Plant Proteins.
- chemical , metabolism : Mixed Function Oxygenases, Plant Proteins, RNA, Messenger.
- Amino Acid Sequence, Chromatography, High Pressure Liquid, Cloning, Molecular, Hybridization, Genetic, Molecular Sequence Data, Phylogeny, Sequence Alignment, Sequence Analysis, Protein.
Abstract
CcGA20ox1 is a gene encoding a GA 20-oxidase, a gibberellin (GA) biosynthetic enzyme, previously isolated from the citrus hybrid Carrizo citrange (Citrus sinensis (L.) Osbeck x Poncirus trifoliata (L.) Raf.). Southern blot analysis of genomic DNA of Carrizo citrange with CcGA20ox1 suggested the presence in the hybrid of another gene encoding another GA 20-oxidase. A cDNA clone from this new gene (CcGA20ox2) was isolated using RNA from the other parent C. sinensis. CcGA20ox2 encoded a protein of 372 amino acids that showed 67.1% identity with CcGA20ox1, and its expression product catalyzed the in vitro conversion of GA12 to GA9, confirming that it corresponds to another active GA20ox. Amplification of genomic DNA and isolation of genomic clones of CcGA20ox1 and CcGA20ox2 revealed that the parental sources of these genes in the hybrid were P. trifoliata and C. sinensis, respectively. The sequences of CcGA20ox1 and CcGA20ox2 showed that both genes contained two introns, which are also conserved in GA20ox genes of other species like Arabidopsis thaliana L., Pisum sativum L. and Solanum lycopersicum L. Determination of transcript levels in the Carrizo citrange hybrid by quantitative real-time polymerase chain reaction showed that CcGA20ox1 was expressed mainly in internodes, leaves and seeds, and CcGA20ox2 in flower buds and flowers at anthesis, with the genes having similar transcript levels in young developing fruits.
DOI: 10.1093/treephys/tpn049
PubMed: 19203975
Affiliations:
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Garcia-Martinez</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19203975</idno><idno type="pmid">19203975</idno><idno type="doi">10.1093/treephys/tpn049</idno><idno type="wicri:Area/PubMed/Corpus">000970</idno><idno type="wicri:Area/PubMed/Curation">000970</idno><idno type="wicri:Area/PubMed/Checkpoint">000970</idno><idno type="wicri:Area/Ncbi/Merge">000944</idno><idno type="wicri:Area/Ncbi/Curation">000944</idno><idno type="wicri:Area/Ncbi/Checkpoint">000944</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization of gibberellin 20-oxidases in the citrus hybrid Carrizo citrange.</title><author><name sortKey="Huerta, Laura" sort="Huerta, Laura" uniqKey="Huerta L" first="Laura" last="Huerta">Laura Huerta</name><affiliation wicri:level="2"><nlm:affiliation>Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avda de los Naranjos s/n, 46022 Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avda de los Naranjos s/n, 46022 Valencia</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Garcia Lor, Andres" sort="Garcia Lor, Andres" uniqKey="Garcia Lor A" first="Andres" last="Garcia-Lor">Andres Garcia-Lor</name></author><author><name sortKey="Garcia Martinez, Jose L" sort="Garcia Martinez, Jose L" uniqKey="Garcia Martinez J" first="Jose L" last="Garcia-Martinez">Jose L. Garcia-Martinez</name></author></analytic><series><title level="j">Tree physiology</title><idno type="ISSN">0829-318X</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Chromatography, High Pressure Liquid</term><term>Citrus (enzymology)</term><term>Citrus (genetics)</term><term>Cloning, Molecular</term><term>Hybridization, Genetic</term><term>Mixed Function Oxygenases (chemistry)</term><term>Mixed Function Oxygenases (genetics)</term><term>Mixed Function Oxygenases (metabolism)</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>RNA, Messenger (metabolism)</term><term>Sequence Alignment</term><term>Sequence Analysis, Protein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Mixed Function Oxygenases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term><term>Mixed Function Oxygenases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mixed Function Oxygenases</term><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Chromatography, High Pressure Liquid</term><term>Cloning, Molecular</term><term>Hybridization, Genetic</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Alignment</term><term>Sequence Analysis, Protein</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">CcGA20ox1 is a gene encoding a GA 20-oxidase, a gibberellin (GA) biosynthetic enzyme, previously isolated from the citrus hybrid Carrizo citrange (Citrus sinensis (L.) Osbeck x Poncirus trifoliata (L.) Raf.). Southern blot analysis of genomic DNA of Carrizo citrange with CcGA20ox1 suggested the presence in the hybrid of another gene encoding another GA 20-oxidase. A cDNA clone from this new gene (CcGA20ox2) was isolated using RNA from the other parent C. sinensis. CcGA20ox2 encoded a protein of 372 amino acids that showed 67.1% identity with CcGA20ox1, and its expression product catalyzed the in vitro conversion of GA12 to GA9, confirming that it corresponds to another active GA20ox. Amplification of genomic DNA and isolation of genomic clones of CcGA20ox1 and CcGA20ox2 revealed that the parental sources of these genes in the hybrid were P. trifoliata and C. sinensis, respectively. The sequences of CcGA20ox1 and CcGA20ox2 showed that both genes contained two introns, which are also conserved in GA20ox genes of other species like Arabidopsis thaliana L., Pisum sativum L. and Solanum lycopersicum L. Determination of transcript levels in the Carrizo citrange hybrid by quantitative real-time polymerase chain reaction showed that CcGA20ox1 was expressed mainly in internodes, leaves and seeds, and CcGA20ox2 in flower buds and flowers at anthesis, with the genes having similar transcript levels in young developing fruits.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Communauté valencienne</li></region></list><tree><noCountry><name sortKey="Garcia Lor, Andres" sort="Garcia Lor, Andres" uniqKey="Garcia Lor A" first="Andres" last="Garcia-Lor">Andres Garcia-Lor</name><name sortKey="Garcia Martinez, Jose L" sort="Garcia Martinez, Jose L" uniqKey="Garcia Martinez J" first="Jose L" last="Garcia-Martinez">Jose L. Garcia-Martinez</name></noCountry><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Huerta, Laura" sort="Huerta, Laura" uniqKey="Huerta L" first="Laura" last="Huerta">Laura Huerta</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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