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Adventive plants from ovules and nucelli in Citrus

Identifieur interne : 004F23 ( Main/Merge ); précédent : 004F22; suivant : 004F24

Adventive plants from ovules and nucelli in Citrus

Auteurs : J. Kochba [Israël] ; P. Spiegel-Roy [Israël] ; Hannah Safran [Israël]

Source :

Planta [ 0032-0935 ] ; 1972-09-01.

RBID : ISTEX:ACD9DED1EC260B775E405427DEA2F777448BD7A3

Abstract

Summary: 1- to 8-week-old ovules and nucelli from three Citrus cultivars—Shamouti and Valencia (Citrus sinensis) oranges and Marsh Seedless (C. paradisi) grapefruit—were cultured in vitro. No embryo differentiation was observed in the explants prior to culture. The Shamouti ovules had degenerated and were apparently unfertilized. Embryoids formed on Murashige and Tucker nutrient medium supplemented with 500 mg/l malt extract. Whole plants developed on the same basal medium supplemented with kinetin and indole-3-acetic acid (IAA), coconut milk or gibberellic acid (GA3). A higher kinetin/IAA ratio or the addition of coconut milk favoured stem elongation more than root formation while a lower kinetin/IAA ratio favoured root formation and inhibited stem elongation. The addition of GA3 to the basal medium stimulated rooting and stem elongation. These results can be of aid in mutation research, allowing irradiation at stages prior to embryonic development.

Url:

https://api.istex.fr/document/ACD9DED1EC260B775E405427DEA2F777448BD7A3/fulltext/pdf

DOI: 10.1007/BF00388101

Links to Exploration step

ISTEX:ACD9DED1EC260B775E405427DEA2F777448BD7A3

Le document en format XML

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<front><div type="abstract" xml:lang="en">Summary: 1- to 8-week-old ovules and nucelli from three Citrus cultivars—Shamouti and Valencia (Citrus sinensis) oranges and Marsh Seedless (C. paradisi) grapefruit—were cultured in vitro. No embryo differentiation was observed in the explants prior to culture. The Shamouti ovules had degenerated and were apparently unfertilized. Embryoids formed on Murashige and Tucker nutrient medium supplemented with 500 mg/l malt extract. Whole plants developed on the same basal medium supplemented with kinetin and indole-3-acetic acid (IAA), coconut milk or gibberellic acid (GA3). A higher kinetin/IAA ratio or the addition of coconut milk favoured stem elongation more than root formation while a lower kinetin/IAA ratio favoured root formation and inhibited stem elongation. The addition of GA3 to the basal medium stimulated rooting and stem elongation. These results can be of aid in mutation research, allowing irradiation at stages prior to embryonic development.</div>
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Adventive plants from ovules and nucelli in Citrus

Adventive plants from ovules and nucelli in Citrus

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